CN100451612C - Kit for rapid diagnosis of sperm concentration, preparation, and method of application - Google Patents
Kit for rapid diagnosis of sperm concentration, preparation, and method of application Download PDFInfo
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- CN100451612C CN100451612C CNB2007100629850A CN200710062985A CN100451612C CN 100451612 C CN100451612 C CN 100451612C CN B2007100629850 A CNB2007100629850 A CN B2007100629850A CN 200710062985 A CN200710062985 A CN 200710062985A CN 100451612 C CN100451612 C CN 100451612C
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- test hole
- seminal fluid
- thieving paper
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Abstract
A kit used for quickly diagnosing concentration of semen consists of semen collection box, cell-stain liquid and test plate. It is featured as forming said semen collection box by plastic collection box and semen liquefier; forming said test plate by shell and base plate including water absorption paper, filtering film and standard color code; arranging two holes of test hole and comparison hole at center of shell.
Description
Technical field
The present invention relates to a kind of in-vitro diagnosis class medicine equipment, specifically is a kind of quick detection kit that is applicable to mankind spermatozoon concentration.
Background technology
The fertility expert report, (no contraceptives) can not be conceived in 1 year for about in the world 15% Mr. and Mrs, and 40% in these cases are because male factor causes.Also have Andriatrics Dept. expert report, the incidence of disease of male sterility is approximately 6-12%.Above-mentioned report, the crowd that male sterility is described is a sizable crowd.
The main method of current diagnosis male sterility is the microscopically diagnosis of seminal fluid, and promptly sperm morphological detects, and this also is the goldstandard of male sterility diagnosis.But there are many shortcomings in this method, such as: because Chinese traditional concept is shyly gone the disease of hospital therapy male sterility aspect, thereby has been incured loss through delay treatment.Even gone to hospital, owing to the condition of getting sperm is limited, be subjected to the restriction of environment, method, time, be difficult for obtaining sample.It is more loaded down with trivial details to go to a hospital to see a doctor, must be through a series of programs such as registering, prescribe.
Summary of the invention
The objective of the invention is in order to overcome the shortcoming of prior art, provide a kind of and can operate at home, quick, easy to use, low-cost kit for rapid diagnosis of sperm concentration.
Second purpose of the present invention provides a kind of preparation and using method of kit for rapid diagnosis of sperm concentration.
For achieving the above object, the present invention includes seminal fluid collecting box, cell dyeing liquid and breadboard, described seminal fluid collecting box collects box by plastics and the seminal fluid liquefier is formed, described breadboard comprises shell, substrate, shell and substrate are that rectangular PVC plastics are made, and there are test hole and two apertures of control wells in shell central authorities, and thieving paper, filtering membrane and standard colour code are arranged on the described substrate, thieving paper and filtering membrane are positioned at the substrate top, the test hole below of shell; Thieving paper is positioned at the filtering membrane below; The standard colors mark is in the substrate top, and below the control wells, standard colors target color is the color that 2,000 ten thousand/ml concn sample of sperm adds the cell dyeing liquid test findings.Described filtering membrane is a glass fibre membrane, and pore diameter range 1-30 micron, size dimension are 10 millimeters * 10 millimeters; Described thieving paper size dimension is consistent with filtering membrane.Described seminal fluid liquefier can be enzyme preparation such as bromelain, pronase or chymotrypsin, and non-enzyme preparation such as dithiothreitol (DTT), working concentration is 1-5mg/ml.Described cell dyeing liquid can be acid, alkalescence and neutral dye cell dyeing liquid, and serge blue for example, peacock green, Yihong etc., working concentration is 0.01-1.0%.Described physiological saline also can be pure water and the destainer that contains acidic materials such as low-concentration acetic acid.
A kind of preparation method of kit for rapid diagnosis of sperm concentration comprises the steps:
Preparation cell dyeing agent solution: stain for cell is added phosphate buffer fully dissolve, filter rearmounted room temperature preservation;
The preparation standard colour code: the sperm sample with the 2000 ten thousand/ml concn of confirming through microscopy is a master sample, add the coloring agent solution that has prepared, according to the color of experimental result, use modern printing technology and prepare the standard colour code, place the control wells below;
Filtering membrane and thieving paper are placed the substrate top successively, the test hole below.
Advantage of the present invention: the concentration that can detect sperm rapidly and accurately, thereby diagnose out the male sex whether can procreate, save a lot of times than traditional in the past diagnostic method, use simply, use and get final product, do not spend hospital admission in pharmacy's purchase, family, make the user not have shy sense, make the man and wife can know the potential fertility Issue of oneself in advance, use this kit to detect, also can be used for sperm bank sperm quality is screened effect after man's sterilization operation.
Description of drawings
The present invention will be further described in detail below in conjunction with accompanying drawing.
Fig. 1 is the structural representation of breadboard of the present invention.
1. substrates, 2. shells, 3. test holes, 4. control wells, 5. filtering membranes, 6. thieving papers, 7. standard colour codes among Fig. 1
Embodiment
As seen from the figure, breadboard of the present invention comprises substrate 1 and shell 2, and there are test hole 3 and 4 two apertures of control wells in shell 2 central authorities, and filtering membrane 5, thieving paper 6 and standard colour code 7 are arranged on the substrate 1, and filtering membrane 5 and thieving paper 6 are positioned at substrate 1 top, test hole 3 belows; Thieving paper 6 is positioned at filtering membrane 5 belows; Standard colour code 7 is positioned at substrate 1 top, control wells 4 belows.
The color of standard colour code 7 is the color that 2,000 ten thousand/ml concn sample of sperm adds stain for cell solution trial result.
A kind of method for preparing the sperm concentration quick detection kit is an example with the serge blue stain for cell:
Preparation concentration is 0.1% methylene blue solution: precision takes by weighing 105 ℃ of biological stain serge blue 100mg that are dried to constant weight, puts in the 1000ml measuring bottle, adds phosphate buffer to scale, shakes up, and filters, and filters rearmounted room temperature preservation;
The preparation standard colour code: the sperm sample with the 2000 ten thousand/ml concn of confirming through microscopy is a master sample, add the methylene blue solution that has prepared, color according to experimental result, prepare the standard colour code, use modern printing technology and prepare the standard colour code, place the control wells below;
Filtering membrane and thieving paper are placed the substrate top successively, the test hole below.
The using method of sperm concentration quick detection kit is as follows:
1, collects semen sample in the seminal fluid collecting box;
2, seminal fluid liquefaction allows seminal fluid liquefy in the seminal fluid collecting box, and the general time is 15 to 30 minutes, is determined at behind the seminal fluid collecting to finish in 12 hours;
3, application of sample is collected 1 seminal fluid with little plastic suction pipe and is added in the test hole, allow test hole inner filtration film fully soak 1 minute and allows thieving paper blot moisture content on the film;
4, add 2 physiological saline to test hole, allow test hole inner filtration film fully soak 1 minute and allow thieving paper blot moisture content on the film;
5, add 2 methylene blue solutions to test hole, allow test hole inner filtration film fully soak 1 minute and allow thieving paper blot moisture content on the film;
6, add 2 physiological saline to test hole, allow test hole inner filtration film fully soak 1 minute, begin to read the result after waiting thieving paper to blot moisture content on the film.To in 4 minutes, read the result, inaccurate above reading the possibility of result after 4 minutes;
7, the change color in viewing test hole:
Positive: the test hole blueness is darker than the color of control wells internal standard colour code, shows sperm concentration 〉=2,000 ten thousand/milliliter;
Negative: the test hole blueness is more of light color than control wells internal standard colour code, shows sperm concentration＜2,000 ten thousand/milliliter;
8, test for the second time:
Per twice test is at interval more than the week.
Twice all negative testee of test findings please arrive the further diagnoses and treatment of hospital.
Claims (7)
1, a kind of kit for rapid diagnosis of sperm concentration, it is characterized in that: comprise seminal fluid collecting box, cell dyeing liquid and breadboard, described seminal fluid collecting box collects box by plastics and the seminal fluid liquefier is formed, described breadboard comprises shell, substrate, shell and substrate are that rectangular PVC plastics are made, and there are test hole and two apertures of control wells in shell central authorities, and thieving paper, filtering membrane and standard colour code are arranged on the described substrate, thieving paper and filtering membrane are positioned at the substrate top, the test hole below of shell; Thieving paper is positioned at the filtering membrane below: the standard colors mark is in the substrate top, and below the control wells, standard colors target color is the color that 2,000 ten thousand/ml concn sample of sperm adds the cell dyeing liquid test findings.
2, a kind of kit for rapid diagnosis of sperm concentration according to claim 1 is characterized in that: described filtering membrane is a glass fibre membrane, and pore diameter range 1-30 micron, size dimension are 10 millimeters * 10 millimeters; Described thieving paper size dimension is consistent with filtering membrane.
3, a kind of kit for rapid diagnosis of sperm concentration according to claim 1, it is characterized in that: described seminal fluid liquefier can be enzyme preparation such as bromelain, pronase or chymotrypsin, non-enzyme preparation such as dithiothreitol (DTT), working concentration is 1-5mg/ml.
4, a kind of kit for rapid diagnosis of sperm concentration according to claim 1 is characterized in that: described cell dyeing liquid can be acid, alkalescence and neutral dye cell dyeing liquid, serge blue for example, peacock green, Yihong etc., working concentration is 0.01-1.0%.
5, a kind of method for preparing each described kit for rapid diagnosis of sperm concentration of claim 1-4, comprising following steps:
Preparation cell dyeing agent solution: stain for cell is added phosphate buffer fully dissolve, filter rearmounted room temperature preservation;
The preparation standard colour code: the sperm sample with the 2000 ten thousand/ml concn of confirming through microscopic counting is a master sample, add the coloring agent solution that has prepared, according to the color of experimental result, use modern printing technology and prepare the standard colour code, place the control wells below;
Filtering membrane and thieving paper are placed the substrate top successively, the test hole below.
6, a kind of method of using each described kit for rapid diagnosis of sperm concentration of claim 1-4: comprising following steps:
Collect semen sample in the seminal fluid collecting box;
Seminal fluid liquefaction allows seminal fluid liquefy in the seminal fluid collecting box, and the general time is 15 to 30 minutes, is determined at behind the seminal fluid collecting to finish in 12 hours;
Application of sample is collected 1 seminal fluid with little plastic suction pipe and is added in the test hole, allow test hole inner filtration film fully soak 1 minute and ends thieving paper and blot moisture content on the film;
Add 2 physiological saline to test hole, allow test hole inner filtration film fully soak 1 minute and allow thieving paper blot moisture content on the film;
Add the cell coloring agent, add 2 stain for cell, allow test hole inner filtration film fully soak 1 minute and count thieving paper and blot moisture content on the film to test hole;
Add 2 physiological saline to test hole, allow test hole inner filtration film fully soak 1 minute, begin to read the result after waiting thieving paper to blot moisture content on the film, in 4 minutes, read the result, surpass that to read the possibility of result after 4 minutes inaccurate;
The change color in viewing test hole:
Positive: the test hole color is darker than the color of control wells internal standard colour code, shows sperm concentration 〉=2,000 ten thousand/milliliter;
Negative: the test hole color is more of light color than control wells internal standard colour code, shows sperm concentration＜2,000 ten thousand/milliliter;
Test for the second time:
Per twice test is at interval more than the week.
Twice all negative testee of test findings please arrive the further diagnoses and treatment of hospital.
7, method according to claim 6 is characterized in that: described physiological saline also can be pure water and the destainer that contains acidic materials such as low-concentration acetic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100629850A CN100451612C (en) | 2007-01-24 | 2007-01-24 | Kit for rapid diagnosis of sperm concentration, preparation, and method of application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100629850A CN100451612C (en) | 2007-01-24 | 2007-01-24 | Kit for rapid diagnosis of sperm concentration, preparation, and method of application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101004377A CN101004377A (en) | 2007-07-25 |
| CN100451612C true CN100451612C (en) | 2009-01-14 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2007100629850A Ceased CN100451612C (en) | 2007-01-24 | 2007-01-24 | Kit for rapid diagnosis of sperm concentration, preparation, and method of application |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20170099737A (en) | 2016-02-23 | 2017-09-01 | 노을 주식회사 | Contact-type staining patch and staining method using the same |
| US10371610B2 (en) | 2016-02-23 | 2019-08-06 | Noul Co., Ltd. | Contact-type patch, staining method using the same, and manufacturing method thereof |
| CN107036932A (en) * | 2017-03-22 | 2017-08-11 | 李乐 | Enzymatic liquefaction combines the method and device of centrifugation auxiliary half-quantitative detection sperm concentration |
| CN114057604A (en) * | 2020-08-03 | 2022-02-18 | 深圳先进技术研究院 | Cell nucleus fluorescent dye and dyeing method thereof |
| WO2022027174A1 (en) * | 2020-08-03 | 2022-02-10 | 深圳先进技术研究院 | Cell nucleus fluorescent dye and dyeing method therefor |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2069263U (en) * | 1990-05-23 | 1991-01-16 | 吴小庆 | Spermatozoa measuring plate |
| US5055411A (en) * | 1988-09-09 | 1991-10-08 | Androscore Corporation | Methods used in testing human males for fertility |
| CN2446514Y (en) * | 2000-09-28 | 2001-09-05 | 珠海新爱康生物医学工程有限公司 | Sperm quality detecting analyzer |
| JP2002504666A (en) * | 1998-02-19 | 2002-02-12 | イントロテック | Sperm analysis system |
| CN1399723A (en) * | 1999-08-06 | 2003-02-26 | Imi国际医学创新股份有限公司 | Spectrophotometric measurement in color-based biochemical and immunologigal assays |
| CN1584545A (en) * | 2004-06-16 | 2005-02-23 | 深圳华康生物医学工程有限公司 | Sperm morphology rapid dyeing reagent and method for rapid dyeing sperm |
| CN200996936Y (en) * | 2007-01-24 | 2007-12-26 | 北京望升伟业科技发展有限公司 | Fast sperm-concentration diagnostic reagent kit |
-
2007
- 2007-01-24 CN CNB2007100629850A patent/CN100451612C/en not_active Ceased
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5055411A (en) * | 1988-09-09 | 1991-10-08 | Androscore Corporation | Methods used in testing human males for fertility |
| CN2069263U (en) * | 1990-05-23 | 1991-01-16 | 吴小庆 | Spermatozoa measuring plate |
| JP2002504666A (en) * | 1998-02-19 | 2002-02-12 | イントロテック | Sperm analysis system |
| CN1399723A (en) * | 1999-08-06 | 2003-02-26 | Imi国际医学创新股份有限公司 | Spectrophotometric measurement in color-based biochemical and immunologigal assays |
| CN2446514Y (en) * | 2000-09-28 | 2001-09-05 | 珠海新爱康生物医学工程有限公司 | Sperm quality detecting analyzer |
| CN1584545A (en) * | 2004-06-16 | 2005-02-23 | 深圳华康生物医学工程有限公司 | Sperm morphology rapid dyeing reagent and method for rapid dyeing sperm |
| CN200996936Y (en) * | 2007-01-24 | 2007-12-26 | 北京望升伟业科技发展有限公司 | Fast sperm-concentration diagnostic reagent kit |
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| CN101004377A (en) | 2007-07-25 |
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Decision date of declaring invalidation: 20111230 Decision number of declaring invalidation: 17852 Granted publication date: 20090114 |