CN100584857C - 高纯度白蛋白生产方法 - Google Patents
高纯度白蛋白生产方法 Download PDFInfo
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- CN100584857C CN100584857C CN03127743A CN03127743A CN100584857C CN 100584857 C CN100584857 C CN 100584857C CN 03127743 A CN03127743 A CN 03127743A CN 03127743 A CN03127743 A CN 03127743A CN 100584857 C CN100584857 C CN 100584857C
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- 229940033355 lauric acid Drugs 0.000 description 1
- 150000004668 long chain fatty acids Chemical class 0.000 description 1
- 108010013207 macro aggregates of albumin Proteins 0.000 description 1
- 229910001425 magnesium ion Inorganic materials 0.000 description 1
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 1
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 239000012982 microporous membrane Substances 0.000 description 1
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- 210000004165 myocardium Anatomy 0.000 description 1
- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 description 1
- 210000004898 n-terminal fragment Anatomy 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen(.) Chemical compound [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 238000006384 oligomerization reaction Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- LCCNCVORNKJIRZ-UHFFFAOYSA-N parathion Chemical compound CCOP(=S)(OCC)OC1=CC=C([N+]([O-])=O)C=C1 LCCNCVORNKJIRZ-UHFFFAOYSA-N 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- TZMFJUDUGYTVRY-UHFFFAOYSA-N pentane-2,3-dione Chemical compound CCC(=O)C(C)=O TZMFJUDUGYTVRY-UHFFFAOYSA-N 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 108010045897 polyalbumin Proteins 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 235000007715 potassium iodide Nutrition 0.000 description 1
- 229960004839 potassium iodide Drugs 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- QQONPFPTGQHPMA-UHFFFAOYSA-N propylene Natural products CC=C QQONPFPTGQHPMA-UHFFFAOYSA-N 0.000 description 1
- 125000004805 propylene group Chemical group [H]C([H])([H])C([H])([*:1])C([H])([H])[*:2] 0.000 description 1
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- MFBOGIVSZKQAPD-UHFFFAOYSA-M sodium butyrate Chemical compound [Na+].CCCC([O-])=O MFBOGIVSZKQAPD-UHFFFAOYSA-M 0.000 description 1
- 229910001415 sodium ion Inorganic materials 0.000 description 1
- 239000002195 soluble material Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
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- 238000003860 storage Methods 0.000 description 1
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- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- CRKADHVTAQCXRA-UHFFFAOYSA-K trisodium;phosphate;dihydrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]P([O-])([O-])=O CRKADHVTAQCXRA-UHFFFAOYSA-K 0.000 description 1
- 238000000825 ultraviolet detection Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000011735 vitamin B7 Substances 0.000 description 1
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- 238000005303 weighing Methods 0.000 description 1
- RZLVQBNCHSJZPX-UHFFFAOYSA-L zinc sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Zn+2].[O-]S([O-])(=O)=O RZLVQBNCHSJZPX-UHFFFAOYSA-L 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70571—Receptors; Cell surface antigens; Cell surface determinants for neuromediators, e.g. serotonin receptor, dopamine receptor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/08—Plasma substitutes; Perfusion solutions; Dialytics or haemodialytics; Drugs for electrolytic or acid-base disorders, e.g. hypovolemic shock
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
- C07K14/765—Serum albumin, e.g. HSA
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
Abstract
Description
# | 保留时间(min) | 面积(uV.sec) | 高(uV) |
1 | 0.800 | 3459686 | 219122 |
2 | 1.667 | 418606 | 33569 |
3 | 2.150 | 77883335 | 1963630 |
4 | 3.000 | 6293258 | 122295 |
5 | 20.433 | 297608 | 14424 |
6 | 22.900 | 205822 | 14601 |
7 | 27.567 | 150851 | 10835 |
8 | 31.117 | 2213883 | 170938 |
9 | 37.983 | 164710 | 15088 |
10 | 39.267 | 347946 | 29879 |
11 | 41.750 | 107515 | 8402 |
12 | 42.783 | 2303024 | 192911 |
13 | 43.217 | 139744 | 14141 |
14 | 43.457 | 254521 | 23979 |
15 | 50.467 | 152805 | 13226 |
16 | 50.950 | 162364 | 12577 |
17 | 56.533 | 5753796 | 83674 |
# | 保留时间(min) | 面积(uV.sec) | 高(uV) |
1 | 0.350 | 244385 | 23957 |
2 | 0.633 | 607880 | 45310 |
3 | 0.783 | 3239730 | 243477 |
4 | 0.983 | 1072033 | 158146 |
5 | 2.233 | 76773569 | 2038028 |
6 | 2.933 | 6634089 | 182363 |
7 | 3.733 | 2812688 | 95459 |
8 | 12.483 | 818540 | 20185 |
9 | 12.650 | 218748 | 22750 |
10 | 14.150 | 5423715 | 98336 |
11 | 16.333 | 423403 | 17460 |
12 | 16.633 | 688525 | 24538 |
13 | 17.550 | 2301309 | 84781 |
14 | 18.033 | 1145045 | 47806 |
15 | 19.750 | 672721 | 21562 |
16 | 20.233 | 87799 | 9760 |
17 | 20.700 | 272171 | 13003 |
18 | 21.100 | 862146 | 55792 |
19 | 21.967 | 166471 | 8928 |
20 | 22.883 | 1381445 | 97660 |
21 | 23.583 | 1112632 | 89851 |
22 | 24.000 | 4740347 | 419780 |
23 | 24.417 | 352486 | 26374 |
24 | 24.917 | 171279 | 14625 |
25 | 25.133 | 99734 | 11473 |
26 | 25.267 | 133911 | 10515 |
27 | 25.667 | 223556 | 11854 |
# | 保留时间(min) | 面积(uV.sec) | 高(uV) |
29 | 26.600 | 93906 | 7957 |
30 | 26.817 | 223113 | 18326 |
31 | 27.250 | 303831 | 29461 |
32 | 27.533 | 124218 | 12710 |
33 | 27.783 | 5747091 | 561629 |
34 | 28.550 | 1383761 | 119772 |
35 | 29.033 | 390986 | 33455 |
36 | 29.417 | 182131 | 12713 |
37 | 29.833 | 181333 | 12584 |
38 | 30.183 | 478320 | 30155 |
39 | 30.583 | 1048945 | 58465 |
40 | 31.067 | 3454425 | 214489 |
41 | 31.983 | 168275 | 8663 |
42 | 32.717 | 651406 | 43161 |
43 | 33.150 | 1142221 | 102588 |
44 | 34.017 | 420756 | 23883 |
45 | 35.100 | 115704 | 10008 |
46 | 37.033 | 166588 | 9468 |
47 | 38.267 | 145731 | 8078 |
48 | 38.983 | 781209 | 54029 |
49 | 41.800 | 86967 | 8868 |
50 | 48.883 | 95416 | 8522 |
51 | 50.267 | 174159 | 16737 |
52 | 50.483 | 176115 | 15573 |
53 | 51.267 | 158727 | 13701 |
54 | 52.183 | 297278 | 25795 |
55 | 56.533 | 5846645 | 85710 |
样品 | 序列 |
脱-Leu rHA | LVAASQAALGAWAVARDLGEENFK |
HSA标准品 | AWAVARDLGEENFK+about5%LVAASQAALG |
HSA 30℃下12周 | AWAVARDLGEENFK |
rHA4℃下6个月 | AWAVARDLGEENFK |
30℃下6个月 | AWAVARDLGEENFK |
合成的全长和截断C末端肽的混合物 | 1013-LVAASQAALGL900-LVAASQAALG |
脱-Len rHA | 673-AWAVAR900-LVAASQAALG951-DLGEENFK1028-?1140-? |
HSA标准品 | 673-AWAVAR900-LVAASQAALG951-DLGEENFK1028-?1140-? |
rHA于30℃下6个月 | 673-AWAVAR900-LVAASQAALG1028-?1140-?951-无信号 |
金属离子 | 波长 | 原子化温度℃ |
Zn | 213.9 | 1800 |
Cu | 327.4 | 2300 |
Fe | 248.8 | 2400 |
A l | 309.8 | 2500 |
Mn | 279.8 | 2200 |
样品 | A<sub>350</sub> | A<sub>403</sub> | A<sub>500</sub> |
1 | 9.95 | 4.10 | 0.8 |
2 | 9.25 | 5.36 | 1.1 |
3 | 7.40 | 3.26 | 0.6 |
4 | 7.20 | 3.60 | 0.6 |
5 | 8.68 | 4.08 | 0.8 |
6 | 11.45 | 6.26 | 1.2 |
7 | 7.20 | 3.70 | 0.8 |
8 | 6.82 | 4.78 | 1.8 |
Claims (4)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US378,859 | 1995-05-25 | ||
US08/378,859 US5728553A (en) | 1992-09-23 | 1995-05-25 | High purity albumin and method of producing |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB961958243A Division CN1198844C (zh) | 1995-05-25 | 1996-02-29 | 高纯度白蛋白生产方法 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB2006101006176A Division CN100443499C (zh) | 1995-05-25 | 1996-02-29 | 高纯度白蛋白生产方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1550504A CN1550504A (zh) | 2004-12-01 |
CN100584857C true CN100584857C (zh) | 2010-01-27 |
Family
ID=23494845
Family Applications (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB2004100575838A Expired - Lifetime CN100455597C (zh) | 1995-05-25 | 1996-02-29 | 高纯度白蛋白生产方法 |
CN03127743A Expired - Lifetime CN100584857C (zh) | 1995-05-25 | 1996-02-29 | 高纯度白蛋白生产方法 |
CNB961958243A Expired - Lifetime CN1198844C (zh) | 1995-05-25 | 1996-02-29 | 高纯度白蛋白生产方法 |
CNB2006101006176A Expired - Lifetime CN100443499C (zh) | 1995-05-25 | 1996-02-29 | 高纯度白蛋白生产方法 |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB2004100575838A Expired - Lifetime CN100455597C (zh) | 1995-05-25 | 1996-02-29 | 高纯度白蛋白生产方法 |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB961958243A Expired - Lifetime CN1198844C (zh) | 1995-05-25 | 1996-02-29 | 高纯度白蛋白生产方法 |
CNB2006101006176A Expired - Lifetime CN100443499C (zh) | 1995-05-25 | 1996-02-29 | 高纯度白蛋白生产方法 |
Country Status (16)
Country | Link |
---|---|
US (6) | US5728553A (zh) |
EP (2) | EP0828759B1 (zh) |
JP (4) | JP4040088B2 (zh) |
KR (2) | KR100566606B1 (zh) |
CN (4) | CN100455597C (zh) |
AT (2) | ATE265469T1 (zh) |
AU (1) | AU698409B2 (zh) |
CA (1) | CA2220923C (zh) |
DE (2) | DE69632353T2 (zh) |
DK (2) | DK0828759T3 (zh) |
ES (2) | ES2156991T3 (zh) |
GR (1) | GR3035651T3 (zh) |
HK (2) | HK1009456A1 (zh) |
MX (1) | MX9709108A (zh) |
PT (2) | PT828759E (zh) |
WO (1) | WO1996037515A1 (zh) |
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Non-Patent Citations (2)
Title |
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The secretion of human seruma lbumin from yeast S.cerevisiae using five different leader sequences. D SLEEP et al.BIOTECHNOLOGY,Vol.8 No.1. 1990 |
The secretion of human seruma lbumin from yeast S.cerevisiae using five different leader sequences. D SLEEP et al.BIOTECHNOLOGY,Vol.8 No.1. 1990 * |
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