CN101779733B - Feed additive for improving collagen in livestock and poultry muscle and production method thereof and application thereof - Google Patents
Feed additive for improving collagen in livestock and poultry muscle and production method thereof and application thereof Download PDFInfo
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- CN101779733B CN101779733B CN 201010134182 CN201010134182A CN101779733B CN 101779733 B CN101779733 B CN 101779733B CN 201010134182 CN201010134182 CN 201010134182 CN 201010134182 A CN201010134182 A CN 201010134182A CN 101779733 B CN101779733 B CN 101779733B
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Abstract
The invention relates to the technical field of biological product, in particular to a feed additive for improving collagen in livestock and poultry muscle and a production method thereof and application thereof. The feed additive for improving the collagen in livestock and poultry muscle comprises the active ingredients in percentage by weight: 0.3 to 5 percent of glycine, 0.5 to 7 percent of hydroxylysine, 0.6 to 7 percent of praline, 0.2 to 3 percent of hydroxyproline and 85 to 92 percent of starch (all the contents of the ingredients are calculated at the dry product). The production process is simple, the operation is easy, the product conversion rate is high, the fermentation period is short, no chemical component is added during the production process, and the product is free from toxic and side effect and is environment-friendly, safe and reliable. The feed additive can effectively improve the content of the collagen in the livestock and poultry muscles.
Description
Technical field:
The present invention relates to biological product technical field, particularly a kind of feed addictive and production method and application that improves collagen in the livestock and poultry muscle.
Background technology:
Scientific research shows, is made up of collagen by 70% in the human body skin composition, and collagen makes cell become plentiful, full, keeps skin elasticity with moist, makes skin keep young.Collagen can also reduce triglycerides and the cholesterol in the human body, improves the puzzlement of female diseases, alleviates the various discomforts of climacteric women.In case the shortage collagen can have a strong impact on internal organs health, even cause various diseases, therefore fully replenishing collagen can be assisted the internal organs nutritional supplementation, effectively prevent disease.
Collagen mainly is present in pork skin, beef tendon, chicken wings, cock skin, pig's feet, fish-skin and the cartilage; Content seldom in the livestock and poultry meat; But livestock and poultry meat and correlated product are requisite food on the mass consumption dining table; Therefore, livestock and poultry meat and the correlated product of developing high collagen content have become extremely urgent problem.
Summary of the invention:
The present invention is directed to the existing in prior technology problem, and a kind of feed addictive of collagen in the livestock and poultry muscle and technical scheme of production technology and application thereof of improving is provided, production technology is simple; Easy to operate, conversion rate of products is high, and fermentation period is short; Do not add any chemical composition in the production process; Product has no side effect, and environmental protection is safe and reliable.And the said feed addictive of this programme can effectively improve the content of collagen in the livestock and poultry muscle.
The present invention realizes through following technical measures:
A kind of feed addictive that improves collagen in the livestock and poultry muscle; It is characterized in that the active ingredient and the percentage by weight thereof that comprise are: glycine 0.3-5%, oxylysine 0.5-7%, proline 0.6-7%; Hydroxyproline 0.2-3%, starch 85-92% (above content calculates with dry product).
The production technology of the feed addictive of collagen in the raising livestock and poultry muscle of the present invention may further comprise the steps:
(1), fermentation tank, seeding tank, slack tank sterilization and pipeline sterilization:
First water flushing pipe and fermentation tank before the production; Rinse well, open each turnover valve of each pipeline and fermentation tank, seeding tank except that waterpipe then, feed the steam of 0.13-0.14 MPa then; Let steam and each pipeline valve UNICOM; Discharged steam 20-25 minute, and closed each drain tap then, let the steam pressure of system the inside remain on the 0.13-0.14 MPa; Keep just can reaching in 50-55 minute the effect of thorough sterilization, it is subsequent use by cooling to 28 ℃-30 ℃ inside the system naturally to close the steam main valve then.
(2), the rhodotorula mucilaginosa bacterial classification is cultivated:
Each composition percentage by weight of culture medium:
Cerelose 10%, dipotassium hydrogen phosphate 0.25%, yeast extract 0.25%, raw soya bean powder 0.5%, water 89%;
More than mix and dissolve, pH value is 6-6.5;
Concrete operations:
Get 500 milliliters of triangular flasks, 150 milliliters of every bottled above nutrient solutions, the sealing of triangular flask bottleneck is put into medical disinfecting and is sterilized; Energized, when sterilizer inner vapor pressure reached 0.05 MPa, the air bleeding valve of opening on the sterilizer was discharged the cold air in the sterilizer, when Pressure gauge points to zero; Close air bleeding valve, continue heating, when sterilizer inner vapor pressure reaches the 0.14-0.15 MPa; Keep to reach in 35-40 minute the effect of thorough sterilization, stop heating, let it lower the temperature naturally; When the Pressure gauge of sterilizer points to zero, open air bleeding valve, open the triangular flask that sterilizer takes out the bacterium of having gone out again; Be put into and cool to 28 ℃-30 ℃ on the superclean bench naturally, under the germ-free condition, each triangular flask inserts the Rhodotorula mucilaginose 0.1-0.2 gram of buying; Seal bottleneck then, be put in the biochemical incubator of electric heating, got final product in 36-40 hour cultivating under the condition of 28 ℃-30 ℃ of temperature (more than be called the triangular flask liquid strain);
(3) the seeding tank bacterial classification is cultivated:
Each composition percentage by weight of culture medium:
Cerelose 3%, dipotassium hydrogen phosphate 0.5%, yeast extract 2%, raw soya bean powder 3%, water 91.5%;
Concrete operations:
Earlier the water in the culture medium prescription is thrown in the seeding tank of the bacterium of having gone out, started mixer, add cerelose, dipotassium hydrogen phosphate, yeast extract, raw soya bean powder more successively; Add the back stirring and fully dissolved in 30-35 minute, mixer Revolution Per Minute 150 changes, and closes charging aperture then; The feeding steam is heated, and when steam pressure in the seeding tank reaches the 0.14-0.15 MPa, keeps just can reaching in 55-60 minute the effect of thorough sterilization; Feed cooling water circulation then, when the culture medium in the seeding tank drops to 28 ℃-30 ℃, under aseptic condition; Open the inoculation cap, in seeding tank, insert the triangular flask liquid strain, each hundred kilograms of seed tank culture liquid inserts triangular flask liquid strain 1500-2000 milliliter; Build the inoculation cap then, the beginning seed culture;
Condition of culture: the pH value nature, 28 ℃-30 ℃ of temperature, mixer Revolution Per Minute 120-140 changes, incubation time 30-35 hour;
(4) fermentation tank culture:
Each composition percentage by weight of culture medium:
Cerelose 3%, dipotassium hydrogen phosphate 0.2%, yeast extract 1%, raw soya bean powder 5%, corn flour 4%, wheat bran skin 8%, water 78.8%;
Concrete operations:
Earlier the water in the culture medium prescription is thrown in the fermentation tank of slack tank sterilization, started mixer, Revolution Per Minute 150-180 changes; Add raw soya bean powder, corn flour, yeast extract, wheat bran skin, glucose, dipotassium hydrogen phosphate more successively, add the continued stirring and made it in 30 minutes evenly, the steam that feeds the 0.14-0.15 MPa then begins to heat; When steam pressure in the fermentation tank reaches the 0.14-0.15 MPa, keep just can reaching in 55-60 minute the effect of thorough sterilization, close steam then; Feed the running water cooling by the jar interlayer, when temperature in the jar drops to 28 ℃-30 ℃, begin to connect the cultured Rhodotorula mucilaginose of seeding tank, at first improve seeding tank pressure when 0.1 MPa; Fermentation tank tank pressure 0.05 MPa; Open the inoculation pipeline valve, the seeding tank seed is transported in the fermentation tank goes, the seed consumption is between the 3%-5%;
Fermentation condition: pH value nature; 28 ℃-30 ℃ of temperature; Mixer Revolution Per Minute 200-220 changes, and cultivates to begin to measure glycine content in 30 hours, whenever measures once at a distance from two hours later on; When glycine content reaches 2.8%-3% in the zymotic fluid, (calculate) and just reach fermentation termination, can reach fermentation and finishing in general 40-45 hour with dry product;
(5) finished product extracts:
Zymotic fluid is warmed to 50 ℃-55 ℃, centrifugal with the yeast centrifuge, discard clear liquid and collect wet yeast, between 50 ℃-60 ℃, use the air blast drying machine drying, when moisture between 10%-12%, ultramicro grinding is finished product.
Consumption and the effect of this feed addictive in animal and fowl fodder is:
In livestock feed, add 0.5%-0.8%; In bird feed, add 0.6%-1%, can reach the effect that improves collagen in the livestock and poultry muscle.
Object lesson is following:
1, in pig feed, adds additive 0.5%-0.8% according to the invention by weight percentage; According to normal breed; Can improve collagen content in the pork to 3%-4.8%; Contain in the common pork between the about 0.5%-0.6% of collagen, compare with common pork and improved 6-8 doubly, do not influence growth (test is the Shanghai White pig with pig variety) in the breeding process.
2, in the chicken feed by weight percentage add additive 0.6%-1% according to the invention; According to normal breed; Can improve collagen content in the chicken to 0.75%-1.2%; Contain in the common chicken between the about 0.15%-0.2% of collagen, compare with common chicken and improved 5-6 doubly, do not influence growth (test is hypo sieve fryer with chicken breed) in the breeding process.
This feed addictive is a solid, powdery, directly adds to by consumption and mixes thoroughly in the feed and can feed.
Beneficial effect of the present invention can learn that through foregoing description this programme production technology is simple, and is easy to operate; Conversion rate of products is high, and fermentation period is short, does not add any chemical composition in the production process; Employed bacterial classification is the food-grade barms that uses in the world, and properties of product are stablized, had no side effect, environmental protection; Safe and reliable, can effectively improve the content of collagen in the livestock and poultry muscle.There are not waste water, toxic emission in the production.
The specific embodiment:
For further specifying the present invention, below specifically set forth through several embodiment.
Embodiment 1
Main production equipments:
Bacterial classification culture device: the biochemical incubator of electric heating, superclean bench, medical sterilization pot.
Zymolysis Equipment: stirred-tank fermenter, seeding tank.
Preliminary treatment and finished product equipment: yeast centrifuge, blast drier, micronizer.
Production technology:
(1), fermentation tank, seeding tank, slack tank sterilization and pipeline sterilization:
First water flushing pipe and fermentation tank are rinsed well before the production, open each turnover valve of each pipeline and fermentation tank, seeding tank except that waterpipe then; Feed the steam of 0.13 MPa then, let steam and each pipeline valve UNICOM, discharged steam 20 minutes; Close each drain tap then; Let the steam pressure of system the inside remain on 0.13 MPa, keep just can reaching in 50 minutes the effect of thorough sterilization, it is subsequent use by cooling to 28 ℃ inside the system naturally to close the steam main valve then.
(2), the rhodotorula mucilaginosa bacterial classification is cultivated:
Each composition percentage by weight of culture medium:
Cerelose 10%, dipotassium hydrogen phosphate 0.25%, yeast extract 0.25%, raw soya bean powder 0.5%, water 89%;
More than mix and dissolve, pH value is 6-6.5;
Concrete operations:
Get 500 milliliters of triangular flasks, 150 milliliters of every bottled above nutrient solutions, the sealing of triangular flask bottleneck is put into medical disinfecting and is sterilized; Energized, when sterilizer inner vapor pressure reached 0.05 MPa, the air bleeding valve of opening on the sterilizer was discharged the cold air in the sterilizer, when Pressure gauge points to zero; Close air bleeding valve, continue heating, when sterilizer inner vapor pressure reaches 0.14 MPa; Keep to reach in 35 minutes the effect of thorough sterilization, stop heating, let it lower the temperature naturally; When the Pressure gauge of sterilizer points to zero, open air bleeding valve, open the triangular flask that sterilizer takes out the bacterium of having gone out again; Be put into and cool to 28 ℃ on the superclean bench naturally, under the germ-free condition, each triangular flask inserts Rhodotorula mucilaginose 0.1 gram of buying; Seal bottleneck then, be put in the biochemical incubator of electric heating, got final product in 36 hours cultivating under the condition of 28 ℃ of temperature (more than be called the triangular flask liquid strain);
(3) the seeding tank bacterial classification is cultivated:
Each composition percentage by weight of culture medium:
Cerelose 3%, dipotassium hydrogen phosphate 0.5%, yeast extract 2%, raw soya bean powder 3%, water 91.5%;
Concrete operations:
Earlier the water in the culture medium prescription is thrown in the seeding tank of the bacterium of having gone out, started mixer, add cerelose, dipotassium hydrogen phosphate, yeast extract, raw soya bean powder more successively; Add the back stirring and fully dissolved in 30 minutes, mixer Revolution Per Minute 150 changes, and closes charging aperture then; The feeding steam is heated, and when steam pressure in the seeding tank reaches 0.14 MPa, keeps just can reaching in 55 minutes the effect of thorough sterilization; Feed cooling water circulation then, when the culture medium in the seeding tank drops to 28 ℃, under aseptic condition; Open the inoculation cap, in seeding tank, insert the triangular flask liquid strain, each hundred kilograms of seed tank culture liquid inserts 1500 milliliters of triangular flask liquid strains; Build the inoculation cap then, the beginning seed culture;
Condition of culture: the pH value nature, 28 ℃ of temperature, mixer Revolution Per Minute 120 changes incubation time 30 hours;
(4) fermentation tank culture:
Each composition percentage by weight of culture medium:
Cerelose 3%, dipotassium hydrogen phosphate 0.2%, yeast extract 1%, raw soya bean powder 5%, corn flour 4%, wheat bran skin 8%, water 78.8%;
Concrete operations:
Earlier the water in the culture medium prescription is thrown in the fermentation tank of slack tank sterilization, started mixer, Revolution Per Minute 150 changes; Add raw soya bean powder, corn flour, yeast extract, wheat bran skin, glucose, dipotassium hydrogen phosphate more successively, add the continued stirring and made it in 30 minutes evenly, the steam that feeds 0.14 MPa then begins to heat; When steam pressure in the fermentation tank reaches 0.14 MPa, keep just can reaching in 55 minutes the effect of thorough sterilization, close steam then; Feed the running water cooling by the jar interlayer, when temperature in the jar drops to 28 ℃, begin to connect the cultured Rhodotorula mucilaginose of seeding tank, at first improve seeding tank pressure when 0.1 MPa; Fermentation tank tank pressure 0.05 MPa; Open the inoculation pipeline valve, the seeding tank seed is transported in the fermentation tank goes, the seed consumption is 3%;
Fermentation condition: pH value nature; 28 ℃ of temperature; Mixer Revolution Per Minute 200 changes, and cultivates to begin to measure glycine content in 30 hours, whenever measures once at a distance from two hours later on; When glycine content reaches 2.8%-3% in the zymotic fluid, (calculate) and just reach fermentation termination, can reach fermentation and finish in 40 hours with dry product;
(5) finished product extracts:
Zymotic fluid is warmed to 50 ℃, centrifugal with the yeast centrifuge, discard clear liquid and collect wet yeast, use the air blast drying machine drying at 50 ℃, when moisture 10% the time, ultramicro grinding is finished product.
Consumption and the effect of this feed addictive in animal and fowl fodder is:
In pig feed, add additive 0.5%-0.8% according to the invention by weight percentage; According to normal breed; Can improve collagen content in the pork to 3%-4.8%; Contain in the common pork between the about 0.5%-0.6% of collagen, compare with common pork and improved 6-8 doubly, do not influence growth (test is the Shanghai White pig with pig variety) in the breeding process.
This feed addictive is a solid, powdery, directly adds to by consumption and mixes thoroughly in the feed and can feed.
Embodiment 2
Main production equipments:
Bacterial classification culture device: the biochemical incubator of electric heating, superclean bench, medical sterilization pot.
Zymolysis Equipment: stirred-tank fermenter, seeding tank.
Preliminary treatment and finished product equipment: yeast centrifuge, blast drier, micronizer.
Production technology:
(1), fermentation tank, seeding tank, slack tank sterilization and pipeline sterilization:
First water flushing pipe and fermentation tank are rinsed well before the production, open each turnover valve of each pipeline and fermentation tank, seeding tank except that waterpipe then; Feed the steam of 0.14 MPa then, let steam and each pipeline valve UNICOM, discharged steam 25 minutes; Close each drain tap then; Let the steam pressure of system the inside remain on 0.14 MPa, keep just can reaching in 55 minutes the effect of thorough sterilization, it is subsequent use by cooling to 30 ℃ inside the system naturally to close the steam main valve then.
(2), the rhodotorula mucilaginosa bacterial classification is cultivated:
Each composition percentage by weight of culture medium:
Cerelose 10%, dipotassium hydrogen phosphate 0.25%, yeast extract 0.25%, raw soya bean powder 0.5%, water 89%;
More than mix and dissolve, pH value is 6-6.5;
Concrete operations:
Get 500 milliliters of triangular flasks, 150 milliliters of every bottled above nutrient solutions, the sealing of triangular flask bottleneck is put into medical disinfecting and is sterilized; Energized, when sterilizer inner vapor pressure reached 0.05 MPa, the air bleeding valve of opening on the sterilizer was discharged the cold air in the sterilizer, when Pressure gauge points to zero; Close air bleeding valve, continue heating, when sterilizer inner vapor pressure reaches 0.15 MPa; Keep to reach in 40 minutes the effect of thorough sterilization, stop heating, let it lower the temperature naturally; When the Pressure gauge of sterilizer points to zero, open air bleeding valve, open the triangular flask that sterilizer takes out the bacterium of having gone out again; Be put into and cool to 30 ℃ on the superclean bench naturally, under the germ-free condition, each triangular flask inserts Rhodotorula mucilaginose 0.2 gram of buying; Seal bottleneck then, be put in the biochemical incubator of electric heating, got final product in 40 hours cultivating under the condition of 30 ℃ of temperature (more than be called the triangular flask liquid strain);
(3) the seeding tank bacterial classification is cultivated:
Each composition percentage by weight of culture medium:
Cerelose 3%, dipotassium hydrogen phosphate 0.5%, yeast extract 2%, raw soya bean powder 3%, water 91.5%;
Concrete operations:
Earlier the water in the culture medium prescription is thrown in the seeding tank of the bacterium of having gone out, started mixer, add cerelose, dipotassium hydrogen phosphate, yeast extract, raw soya bean powder more successively; Add the back stirring and fully dissolved in 35 minutes, mixer Revolution Per Minute 150 changes, and closes charging aperture then; The feeding steam is heated, and when steam pressure in the seeding tank reaches 0.15 MPa, keeps just can reaching in 60 minutes the effect of thorough sterilization; Feed cooling water circulation then, when the culture medium in the seeding tank drops to 30 ℃, under aseptic condition; Open the inoculation cap, in seeding tank, insert the triangular flask liquid strain, each hundred kilograms of seed tank culture liquid inserts 2000 milliliters of triangular flask liquid strains; Build the inoculation cap then, the beginning seed culture;
Condition of culture: the pH value nature, 30 ℃ of temperature, mixer Revolution Per Minute 140 changes incubation time 35 hours;
(4) fermentation tank culture:
Each composition percentage by weight of culture medium:
Cerelose 3%, dipotassium hydrogen phosphate 0.2%, yeast extract 1%, raw soya bean powder 5%, corn flour 4%, wheat bran skin 8%, water 78.8%;
Concrete operations:
Earlier the water in the culture medium prescription is thrown in the fermentation tank of slack tank sterilization, started mixer, Revolution Per Minute 180 changes; Add raw soya bean powder, corn flour, yeast extract, wheat bran skin, glucose, dipotassium hydrogen phosphate more successively, add the continued stirring and made it in 30 minutes evenly, the steam that feeds 0.15 MPa then begins to heat; When steam pressure in the fermentation tank reaches 0.15 MPa, keep just can reaching in 60 minutes the effect of thorough sterilization, close steam then; Feed the running water cooling by the jar interlayer, when temperature in the jar drops to 30 ℃, begin to connect the cultured Rhodotorula mucilaginose of seeding tank, at first improve seeding tank pressure when 0.1 MPa; Fermentation tank tank pressure 0.05 MPa; Open the inoculation pipeline valve, the seeding tank seed is transported in the fermentation tank goes, the seed consumption is 5%;
Fermentation condition: pH value nature; 30 ℃ of temperature; Mixer Revolution Per Minute 220 changes, and cultivates to begin to measure glycine content in 30 hours, whenever measures once at a distance from two hours later on; When glycine content reaches 2.8%-3% in the zymotic fluid, (calculate) and just reach fermentation termination, can reach fermentation and finish in 45 hours with dry product;
(5) finished product extracts:
Zymotic fluid is heated 55 ℃, centrifugal with the yeast centrifuge, discard clear liquid and collect wet yeast, in the time of 50 ℃, use the air blast drying machine drying, when moisture 12% the time, ultramicro grinding is finished product.
Consumption and the effect of this feed addictive in animal and fowl fodder is:
Percentage adds additive 0.6%-1% according to the invention by weight in the chicken feed; According to normal breed; Can improve collagen content in the chicken to 0.75%-1.2%; Contain in the common chicken between the about 0.15%-0.2% of collagen, compare with common chicken and improved 5-6 doubly, do not influence growth (test is hypo sieve fryer with chicken breed) in the breeding process.
This feed addictive is a solid, powdery, directly adds to by consumption and mixes thoroughly in the feed and can feed.
Claims (1)
1. the production method of a dusty yeast, said dusty yeast are used for producing the feed addictive that improves the livestock and poultry muscle collagen, and it is characterized in that: the preparation method of said dusty yeast may further comprise the steps:
(1), fermentation tank, seeding tank, slack tank sterilization and pipeline sterilization:
First water flushing pipe and fermentation tank before the production; Rinse well, open each turnover valve of each pipeline and fermentation tank, seeding tank except that waterpipe then, feed the steam of 0.13-0.14 MPa then; Let steam and each pipeline valve UNICOM; Discharged steam 20-25 minute, and closed each drain tap then, let the steam pressure of system the inside remain on the 0.13-0.14 MPa; Keep just can reaching in 50-55 minute the effect of thorough sterilization, it is subsequent use by cooling to 28 ℃-30 ℃ inside the system naturally to close the steam main valve then;
(2), the rhodotorula mucilaginosa bacterial classification is cultivated:
Each composition percentage by weight of culture medium:
Cerelose 10%, dipotassium hydrogen phosphate 0.25%, yeast extract 0.25%, raw soya bean powder 0.5%, water 89%;
More than mix and dissolve, the pH value is 6-6.5;
Concrete operations:
Get 500 milliliters of triangular flasks, 150 milliliters of every bottled above nutrient solutions, the sealing of triangular flask bottleneck is put into medical disinfecting and is sterilized; Energized, when sterilizer inner vapor pressure reached 0.05 MPa, the air bleeding valve of opening on the sterilizer was discharged the cold air in the sterilizer, when Pressure gauge points to zero; Close air bleeding valve, continue heating, when sterilizer inner vapor pressure reaches the 0.14-0.15 MPa, keep to reach in 35-40 minute the effect of thorough sterilization; Stop heating, let it lower the temperature naturally, when the Pressure gauge of sterilizer points to zero; Open air bleeding valve, open the triangular flask that sterilizer takes out the bacterium of having gone out again, be put into and cool to 28 ℃-30 ℃ on the superclean bench naturally; Under the germ-free condition, each triangular flask inserts the Rhodotorula mucilaginose 0.1-0.2 gram of buying, and seals bottleneck then; Be put in the biochemical incubator of electric heating, cultivation got final product in 36-40 hour under the condition of 28 ℃-30 ℃ of temperature, more than was called the triangular flask liquid strain;
(3) the seeding tank bacterial classification is cultivated:
Each composition percentage by weight of culture medium:
Cerelose 3%, dipotassium hydrogen phosphate 0.5%, yeast extract 2%, raw soya bean powder 3%, water 91.5%;
Concrete operations:
Earlier the water in the culture medium prescription is thrown in the seeding tank of the bacterium of having gone out, started mixer, add cerelose, dipotassium hydrogen phosphate, yeast extract, raw soya bean powder more successively; Add the back stirring and fully dissolved in 30-35 minute, mixer Revolution Per Minute 150 changes, and closes charging aperture then; The feeding steam is heated, and when steam pressure in the seeding tank reaches the 0.14-0.15 MPa, keeps just can reaching in 55-60 minute the effect of thorough sterilization; Feed cooling water circulation then, when the culture medium in the seeding tank drops to 28 ℃-30 ℃, under aseptic condition; Open the inoculation cap, in seeding tank, insert the triangular flask liquid strain, each hundred kilograms of seed tank culture liquid inserts triangular flask liquid strain 1500-2000 milliliter; Build the inoculation cap then, the beginning seed culture;
Condition of culture: pH value nature, 28 ℃-30 ℃ of temperature, mixer Revolution Per Minute 120-140 changes, incubation time 30-35 hour;
(4) fermentation tank culture:
Each composition percentage by weight of culture medium:
Cerelose 3%, dipotassium hydrogen phosphate 0.2%, yeast extract 1%, raw soya bean powder 5%, corn flour 4%, wheat bran skin 8%, water 78.8%;
Concrete operations:
Earlier the water in the culture medium prescription is thrown in the fermentation tank of slack tank sterilization, started mixer, Revolution Per Minute 150-180 changes; Add raw soya bean powder, corn flour, yeast extract, wheat bran skin, glucose, dipotassium hydrogen phosphate more successively, add the continued stirring and made it in 30 minutes evenly, the steam that feeds the 0.14-0.15 MPa then begins to heat; When steam pressure in the fermentation tank reaches the 0.14-0.15 MPa, keep just can reaching in 55-60 minute the effect of thorough sterilization, close steam then; Feed the running water cooling by the jar interlayer, when temperature in the jar drops to 28 ℃-30 ℃, begin to connect the cultured Rhodotorula mucilaginose of seeding tank, at first improve seeding tank pressure when 0.1 MPa; Fermentation tank tank pressure 0.05 MPa; Open the inoculation pipeline valve, the seeding tank seed is transported in the fermentation tank goes, the seed consumption is between the 3%-5%;
Fermentation condition: pH value nature; 28 ℃-30 ℃ of temperature, mixer Revolution Per Minute 200-220 changes, and cultivates to begin to measure glycine content in 30 hours; Whenever measured once at a distance from two hours later on, when glycine content in the zymotic fluid reaches 2.8%-3% with dry product calculating, just reach fermentation termination;
(5) finished product extracts:
Zymotic fluid is warmed to 50 ℃-55 ℃, centrifugal with the yeast centrifuge, discard clear liquid and collect wet yeast, between 50 ℃-60 ℃, use the air blast drying machine drying, when moisture between 10%-12%, ultramicro grinding promptly gets.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201010134182 CN101779733B (en) | 2010-03-29 | 2010-03-29 | Feed additive for improving collagen in livestock and poultry muscle and production method thereof and application thereof |
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| CN 201010134182 CN101779733B (en) | 2010-03-29 | 2010-03-29 | Feed additive for improving collagen in livestock and poultry muscle and production method thereof and application thereof |
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| CN101779733B true CN101779733B (en) | 2012-12-26 |
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| CN102919598A (en) * | 2012-11-08 | 2013-02-13 | 黑龙江省轻工科学研究院 | Preparation method for feed additives enabling laying ducks to produce red-yolk duck eggs |
| CN107048057A (en) * | 2017-01-26 | 2017-08-18 | 张伟 | A kind of ginseng and lucid ganoderma nutrient health feed addictive and preparation method and application |
| CN107223799A (en) * | 2017-07-28 | 2017-10-03 | 烟台市昊星晟信息技术有限公司 | A kind of preparation method of health care chicken feed |
| CN114874940B (en) * | 2022-05-10 | 2023-07-14 | 吉林省农业科学院 | Composite microbial agent, fermented product thereof and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101404998A (en) * | 2006-03-23 | 2009-04-08 | 协和发酵工业株式会社 | Oral agent for promoting synthesis of tissue collagen |
| US7671041B2 (en) * | 2006-04-21 | 2010-03-02 | Diana Naturals | Hydrolysate of avian cartilage, process of preparation and uses thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP3802721B2 (en) * | 1999-11-05 | 2006-07-26 | 株式会社ファンケル | Biological collagen synthesis promoter |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101404998A (en) * | 2006-03-23 | 2009-04-08 | 协和发酵工业株式会社 | Oral agent for promoting synthesis of tissue collagen |
| US7671041B2 (en) * | 2006-04-21 | 2010-03-02 | Diana Naturals | Hydrolysate of avian cartilage, process of preparation and uses thereof |
Non-Patent Citations (2)
| Title |
|---|
| JP特开2001-131084A 2001.05.15 |
| 王焕章.维生素C的生理作用及其在饲料中的应用.《湖南饲料》.2003,(第1期),第23-24页. * |
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