CN102608254A - Paper-based thin layer chromatography plate convenient to curl and cut - Google Patents
Paper-based thin layer chromatography plate convenient to curl and cut Download PDFInfo
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- CN102608254A CN102608254A CN2012100409283A CN201210040928A CN102608254A CN 102608254 A CN102608254 A CN 102608254A CN 2012100409283 A CN2012100409283 A CN 2012100409283A CN 201210040928 A CN201210040928 A CN 201210040928A CN 102608254 A CN102608254 A CN 102608254A
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- layer chromatography
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- substrate plate
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Abstract
The invention discloses a paper-based thin layer chromatography plate convenient to curl and cut, which comprises a paper-based plate and a coating layer which is coated on the paper-based plate, wherein the paper-based plate is a piece of paper of which at least one surface is coated with a polyethylene film, and the coating layer is coated on the polyethylene film of the paper-based plate. According to the paper-based thin layer chromatography plate convenient to curl and cut, the paper coated with the polyethylene film is taken as a base body, so that good flexibility and good mechanical strength can be realized, and a paper cutter or a pair of scissors can be used for cutting randomly. The thin layer chromatography plate disclosed by the invention can realize large-batch coating production, is light and convenient to carry, low in cost, relatively little in waste after being used, and easy to treat, can be curled and folded randomly, is suitable for such specific occasions as curved surfaces and the like, and can realize microminiaturization of thin layer chromatography devices.
Description
Technical field
The present invention relates to the thin-layer chromatography field of materials, be specifically related to a kind of paper substrate plate thin layer chromatography board.
Background technology
Chromatographic technique is to the effective method of complex mixture compartment analysis.Thin-layer chromatography (Thin Layer Chromatography) TLC commonly used representes, is called thin-layer chromatography again, is to belong to the solid-liquid adsorption chromatography.Its point sample spot is little, clear spot indiffusion, disengaging time weak point, good separating effect, highly sensitive, is a kind of efficient, the quick and simple chromatography that development in recent years is got up.It has had both the advantage of column chromatography and paper chromatography, can be applicable to the separation of small amount of sample (several to several micrograms, even 0.01 microgram); When strengthening the adsorbed layer thickening, can separate nearly 500 milligrams sample.Therefore, thin-layer chromatography is widely used in fields such as medicine, chemical industry, environmental protection, food, public security, and especially very big effect is being brought into play in the separation of effective constituent and analysis in natural product chemistry.In addition, when carrying out chemical reaction, thin-layered chromatography can be used to follow the tracks of organic reaction, and the degree that reaction is carried out is judged in the progressively intensification that often utilizes thin-layer chromatography to observe progressively disappearing of raw material spot or product spot.
The substrate that is used for thin-layer chromatography requires that various expansion solvents, chemochromic reagent and temperature are all had stability, again certain mechanical strength should be arranged simultaneously.Substrate commonly used has glass plate, plastic plate and aluminium foil etc.Glass plate volume and weight are bigger, and inconvenience is deposited in transportation, makes use cost always than higher.Plastic base prepares the thin layer chromatography board complicated operation, is mostly the sale of finished product thin layer chromatography board, and its advantage is easy to use, and shortcoming is that corrosion resistivity is poor under the high temperature.The thin layer chromatography board that a lot of technologies fall behind is because thickness is too big, and cutting is wasted time and energy, and uses inconvenience, and the incident that the edge that is cut by glass during use scratches also often takes place.
But Granted publication number discloses a kind of cutting type tlc silica gel thin layer chromatography board for the Chinese patent of CN2355331Y; By substrate be coated on its surperficial silica-gel coating and form; But what substrate adopted is the aluminium sheet of cutting, and this thin layer chromatography board can be cut apart arbitrarily, and easy to carry; But fail to reach the effect of curling arbitrarily, and cost is higher.
Summary of the invention
The invention provides a kind of paper substrate plate thin layer chromatography board, can curl arbitrarily and cutting, easy to carry, cost is low.
A kind of paper substrate plate thin layer chromatography board of curling and cutting be convenient to comprises the paper substrate plate and is coated on the coating layer on the paper substrate plate; Described paper substrate plate is at least simultaneously being coated with the paper of polyethylene film; Described coating layer is coated on the polyethylene film of paper substrate plate.
Described coating layer adopts coating fluid to process, and the mass percent of this each component of coating fluid consists of bonding agent 0.75%～7%, adsorbent 12%～33.25%, water 65%～85%.
Described bonding agent is one or more in polyvinyl alcohol (PVA), sodium carboxymethyl cellulose, the starch, and adsorbent is coated on the paper substrate plate securely, improves the physical strength of chromatoplate.
The particle diameter of described adsorbent is 150 orders～200 orders, to improve separating effect.
Described adsorbent is silica gel, zeyssatite, aluminium oxide, magnesium silicate, calcium silicate, zinc carbonate or glass dust.
The thickness of described paper substrate plate is 0.10-0.30mm.
The thickness of described coating layer is 0.20-1.00mm.
The preparation method of described thin layer chromatography board is: bonding agent is mixed with WS form, after insolubles is removed in filtration, adds adsorbent; Stir; Become pasty state, adopt mode manual or the machine coating to be coated on the described paper substrate plate, cut into the shape that needs after the oven dry again.
In the preparation process of paper substrate plate thin layer chromatography board of the present invention, can also in coating fluid, add some adjuvants according to the needs of different industries, for example forge system gypsum, fluorescence indicator etc.
The present invention compared with prior art has following advantage:
Thin layer chromatography board curling and that cut is convenient in the present invention, uses the paper that is coated with polyethylene film to be matrix, has pliability and excellent mechanical intensity preferably, can cut randomly with paper knife or scissors; Thin layer chromatography board of the present invention can realize large batch of coating production, and carries light; Thin layer chromatography board of the present invention, cost is low, and the discarded object after the use is few relatively, and handles easily; Thin layer chromatography board of the present invention, it is folding to curl arbitrarily, is applicable to specific occasions such as curved surface, can realize the microminiaturization of thin-layer chromatography device.
Description of drawings
Fig. 1 is that the activity of the embodiment of the invention 1 thin layer chromatography board is demarcated synoptic diagram, and wherein a is indigo, and b is a tonyred, and c is lemon yellow, and d is indigo+tonyred+lemon yellow.
Embodiment
Embodiment 1
Take by weighing the 1.0g CMC, be dissolved in the 100ml hot water, under magnetic agitation, make its whole dissolvings, refilter remove insolubles after, adding particle diameter is 200 purpose silica gel 20g, continues to stir, and obtains coating fluid, for use.There is the paper substrate plate of polyethylene film to lie on the platform in the front, more a part of coating fluid is poured on the coboundary of paper substrate plate, coating fluid is coated on the paper substrate plate uniformly with glass bar or scraper.The thickness of paper substrate plate is 0.20mm, and the thickness of dry back coating layer is 0.30mm.With thin layer chromatography board activation 30 minutes in 110 ℃ of environment, place the drying box of drying agent subsequent use.
The thin layer chromatography board that present embodiment is made is cut into the thin layer chromatography board of 4cm * 2cm; Thin layer chromatography board after cutting is carried out performance test to be shown; Thin layer chromatography board after cutting can be recovered original extended configuration after spending bendings with 180, explains that thin layer chromatography board of the present invention has pliability preferably; 5 times the tensile test test result is about 170N/15mm, shows that thin layer chromatography board of the present invention has physical strength preferably.
The thin layer chromatography board of present embodiment preparation is carried out activity demarcate, synoptic diagram is as shown in Figure 1 as a result, and thin layer chromatography board can effectively be separated lemon yellow, indigo, tonyred, and its activity is qualified.
Embodiment 2
Take by weighing the 1.0g polyvinyl alcohol (PVA), be dissolved in the 100ml hot water, under magnetic agitation, make its whole dissolvings, refilter remove insolubles after, adding particle diameter is 200 purpose silica gel 20g, continues to stir, and obtains coating fluid, for use.There is the paper substrate plate of polyethylene film to lie on the platform in the front, a part of coating fluid is poured on the coboundary of paper substrate plate, coating fluid is coated on the paper substrate plate uniformly with glass bar or scraper.The thickness of paper substrate plate is 0.20mm, and the thickness of dry back coating layer is 0.25mm.With thin layer chromatography board activation 30 minutes in 110 ℃ of environment, place the drying box of drying agent subsequent use.
Take by weighing the 1.5g CMC, be dissolved in the 100ml hot water, under magnetic agitation, make its whole dissolvings, refilter remove insolubles after, adding particle diameter is 150 purpose zeyssatite 25g, continues to stir, and obtains coating fluid, for use.There is the paper substrate plate of polyethylene film to lie on the platform in the front, a part of coating fluid is poured on the coboundary of paper substrate plate, coating fluid is coated on the paper substrate plate uniformly with glass bar or scraper.The thickness of paper substrate plate is 0.20mm, and the thickness of dry back coating layer is 0.25mm.With thin layer chromatography board activation 30 minutes in 110 ℃ of environment, place the drying box of drying agent subsequent use.
Embodiment 4
Take by weighing 2.0g starch, be dissolved in the 100ml hot water, under magnetic agitation, make its whole dissolvings, refilter remove insolubles after, adding particle diameter is 200 purpose silica gel 25g, continues to stir, and obtains coating fluid, for use.There is the paper substrate plate of polyethylene film to lie on the platform in the front, a part of coating fluid is poured on the coboundary of paper substrate plate, coating fluid is coated on the paper substrate plate uniformly with glass bar or scraper.The thickness of paper substrate plate is 0.20mm, and the thickness of dry back coating layer is 0.25mm.With thin layer chromatography board activation 30 minutes in 110 ℃ of environment, place the drying box of drying agent subsequent use.
Embodiment 5
Take by weighing 0.5g CMC, 0.5g starch, be dissolved in the 100ml hot water, under magnetic agitation, make its whole dissolvings, refilter remove insolubles after, adding particle diameter is 150 purpose magnesium silicate 25g, continues to stir, and obtains coating fluid, for use.There is the paper substrate plate of polyethylene film to lie on the platform in the front, a part of coating fluid is poured on the coboundary of paper substrate plate, coating fluid is coated on the paper substrate plate uniformly with glass bar or scraper.The thickness of paper substrate plate is 0.20mm, and the thickness of dry back coating layer is 0.25mm.With thin layer chromatography board activation 30 minutes in 110 ℃ of environment, place the drying box of drying agent subsequent use.
Claims (7)
1. be convenient to paper substrate plate thin layer chromatography board curling and that cut for one kind, it is characterized in that, comprise described paper substrate plate and be coated on the coating layer on the paper substrate plate, described paper substrate plate is at least simultaneously being coated with the paper of polyethylene film; Described coating layer is coated on the polyethylene film of paper substrate plate.
2. the paper substrate plate thin layer chromatography board of curling and cutting be convenient to as claimed in claim 1; It is characterized in that described coating layer is processed by coating fluid, the mass percent of this each component of coating fluid consists of bonding agent 0.75%～7%; Adsorbent 12%～33.25%, water 65%～85%.
3. the paper substrate plate thin layer chromatography board of curling and cutting be convenient to as claimed in claim 2 is characterized in that described bonding agent is one or more in polyvinyl alcohol (PVA), sodium carboxymethyl cellulose, the starch.
4. the paper substrate plate thin layer chromatography board of curling and cutting be convenient to as claimed in claim 2 is characterized in that the particle diameter of described adsorbent is 150 orders～200 orders.
5. like claim 2 or the 4 described paper substrate plate thin layer chromatography board of curling and cutting be convenient to, it is characterized in that described adsorbent is silica gel, zeyssatite, aluminium oxide, magnesium silicate, calcium silicate, zinc carbonate or glass dust.
6. the paper substrate plate thin layer chromatography board of curling and cutting be convenient to as claimed in claim 1 is characterized in that the thickness of described paper substrate plate is 0.10-0.30mm.
7. the paper substrate plate thin layer chromatography board of curling and cutting be convenient to as claimed in claim 1 is characterized in that the thickness of described coating layer is 0.20-1.00mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100409283A CN102608254A (en) | 2012-02-22 | 2012-02-22 | Paper-based thin layer chromatography plate convenient to curl and cut |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100409283A CN102608254A (en) | 2012-02-22 | 2012-02-22 | Paper-based thin layer chromatography plate convenient to curl and cut |
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| Publication Number | Publication Date |
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| CN102608254A true CN102608254A (en) | 2012-07-25 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2012100409283A Pending CN102608254A (en) | 2012-02-22 | 2012-02-22 | Paper-based thin layer chromatography plate convenient to curl and cut |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105277553A (en) * | 2015-11-23 | 2016-01-27 | 浙江农林大学 | Ninhydrin/nanometer titanium dioxide compound and preparing method and application thereof |
| CN112251326A (en) * | 2020-11-06 | 2021-01-22 | 贵州师范学院 | Device and cutter for labeling nucleic acid probe by gel cutting and purifying isotope |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3409136A (en) * | 1966-07-07 | 1968-11-05 | Ceskoslovenska Akademie Ved | Separation plate for thin-layer chromatography |
| US3418152A (en) * | 1966-08-01 | 1968-12-24 | Eastman Kodak Co | Method of making a chromatographic element containing a resinous binder and product produced thereby |
| US3418158A (en) * | 1964-06-17 | 1968-12-24 | Eastman Kodak Co | Chromatographic sheets |
| US3492155A (en) * | 1967-09-11 | 1970-01-27 | Eastman Kodak Co | Reinforced flexible chromatographic elements |
| CN2355331Y (en) * | 1998-05-28 | 1999-12-22 | 郭景强 | Cutable thin silica gel chromatographic board |
-
2012
- 2012-02-22 CN CN2012100409283A patent/CN102608254A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3418158A (en) * | 1964-06-17 | 1968-12-24 | Eastman Kodak Co | Chromatographic sheets |
| US3409136A (en) * | 1966-07-07 | 1968-11-05 | Ceskoslovenska Akademie Ved | Separation plate for thin-layer chromatography |
| US3418152A (en) * | 1966-08-01 | 1968-12-24 | Eastman Kodak Co | Method of making a chromatographic element containing a resinous binder and product produced thereby |
| US3492155A (en) * | 1967-09-11 | 1970-01-27 | Eastman Kodak Co | Reinforced flexible chromatographic elements |
| CN2355331Y (en) * | 1998-05-28 | 1999-12-22 | 郭景强 | Cutable thin silica gel chromatographic board |
Non-Patent Citations (4)
| Title |
|---|
| 《色谱》 19870829 何丽一 薄层色谱讲座--第一讲 薄层色谱法的基本技术 352-354 1-7 第5卷, 第6期 * |
| E.P.PRZYBYLOWICZ ET AL.: "Precoated sheets for thin-layer chromatography", 《JOURNAL OF CHROMATOGRAPHY A》, vol. 20, no. 3, 31 December 1965 (1965-12-31), pages 506 - 513, XP026749583, DOI: doi:10.1016/S0021-9673(01)97452-7 * |
| 何丽一: "薄层色谱讲座——第一讲 薄层色谱法的基本技术", 《色谱》, vol. 5, no. 6, 29 August 1987 (1987-08-29), pages 352 - 354 * |
| 廖蓓玲: "薄层层析中薄层板的制备方法", 《河池师专学报( 理科)》, vol. 17, no. 2, 30 June 1997 (1997-06-30), pages 104 - 107 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105277553A (en) * | 2015-11-23 | 2016-01-27 | 浙江农林大学 | Ninhydrin/nanometer titanium dioxide compound and preparing method and application thereof |
| CN105277553B (en) * | 2015-11-23 | 2017-11-24 | 浙江农林大学 | Ninhydrin/nanometer titanium dioxide compound and its production and use |
| CN112251326A (en) * | 2020-11-06 | 2021-01-22 | 贵州师范学院 | Device and cutter for labeling nucleic acid probe by gel cutting and purifying isotope |
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Application publication date: 20120725 |