CN102701460A - Method for processing starch waste water and realizing resourcezation of waste water by one photosynthetic bacterium strain - Google Patents
Method for processing starch waste water and realizing resourcezation of waste water by one photosynthetic bacterium strain Download PDFInfo
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- CN102701460A CN102701460A CN2012101656080A CN201210165608A CN102701460A CN 102701460 A CN102701460 A CN 102701460A CN 2012101656080 A CN2012101656080 A CN 2012101656080A CN 201210165608 A CN201210165608 A CN 201210165608A CN 102701460 A CN102701460 A CN 102701460A
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Abstract
The invention relates to a method for processing starch waste water and realizing resourcezation of waste water by one photosynthetic bacterium strain, relating to the field of development of waste water treatment and resourcezation. The starch waste is used as the object, 200 mg/L-600mg/L malic acid is added in the starch waste water in order to adjust the pH value of the starch waste water into 7.0-9.0, and then 160 mg/L-500mg/L rhodobacter sphaeroides at the exponential phase is added. The starch waste water is processed under the illumination anaerobic condition, a filament lamp with illumination strength of 2000lux-3000lux is used as the light source, and the processing temperature is 25 DEG C to 30 DEG C. With the adoption of the method, the photosynthetic bacterium waste water treatment process is simplified, and meanwhile, the large amount of generated bacteria can be directly and comprehensively utilized, so that the purposes of disposing pollution and generating bacterium resources can be synchronously fulfilled.
Description
Technical field
The present invention relates to sewage treatment area, relate in particular to a kind of method of handling starch wastewater and realizing resource utilization.
Technical background
Starch wastewater is one type of maximum waste water of quantity discharged in the food-processing industry.It is nontoxic, is rich in macromole nutritive substances such as a large amount of starch, protein and Mierocrystalline cellulose.Known starch wastewater treatment process has methods such as coagulating sedimentation, air supporting, materialization-acidication-contact oxidation method, UASB.But the use of these methods can produce a large amount of excess sludges, and its treatment and disposal expense is high, and the resource utilization degree is low, and causes secondary pollution easily.
And utilize photosynthetic bacterium (Photosynthetic bacteria) to handle starch wastewater, not only can remove pollutent, environment purification; Simultaneously, the thalline of recovery can directly be used for industries such as feed processing without pre-treatment; This just can realize sewage recycling effectively, turns waste into wealth.But according to the metabolic characteristic of this bacterium described in the uncle Jie Shi bacterium handbook, it can not utilize the materials such as composition-macromolecular polysaccharide and starch in the starch wastewater.Therefore, need before the photosynthetic bacterium processing section, add pre-treatment, earlier macromolecular substance is degraded to small-molecule substance, handle by photosynthetic bacterium again.But the pre-treatment meeting consumes a large number of nutrients in the sewage, can supply the required starting material of thalli growth thereby reduced; Pretreatment section is easy to generate the assorted bacterium that infects photosynthetic bacterium simultaneously, and then reduces the dominant population status of photosynthetic bacterium in WWT; In addition, pretreatment section can produce a large amount of excess sludges.Therefore, the pre-treatment meeting reduces the resource utilization degree of photosynthetic bacterium sewage treating efficiency and entire treatment technology, and can not avoid secondary pollution problems.
Summary of the invention
1. goal of the invention
The present invention is directed to the photosynthetic bacterium macromolecular substance of can not degrading; Thereby need pretreatment technology; And then problem such as the technical process that causes is complicated, sewage treating efficiency is low, the resource utilization degree is low; Simultaneously for solving traditional serious problem of starch wastewater treatment process secondary pollution, a kind of method that can handle starch wastewater effectively and realize the starch wastewater resource utilization of proposition.
2. technical scheme
Of the present inventionly utilize a strain photosynthetic bacterium to handle starch wastewater and realize that the method for sewage recycling carries out according to following steps: at first in starch wastewater, add oxysuccinic acid 200～600mg/L, the pH value of re-adjustment starch wastewater is 7.0～9.0.In starch wastewater, add the Spherical red antibacterial 160～500mg/L that is in logarithmic phase then.The WWT condition is the illumination anaerobism, adopts incandescent light as light source, and intensity of illumination is 2000～3000lux, and treatment temp is 25～30 ℃.
The present invention provides the Spherical red antibacterial of strain separation and purification from soil, and the RhodobacterSphaeroides that belongs in the photosynthetic bacterium belongs to.The processing of starch wastewater is carried out according to being total to the metabolism principle.Metabolism is meant that mikrobe is to the degraded and the conversion of non-growth matrix under the situation that growth matrix exists altogether.Among the present invention, growth matrix is called oxysuccinic acid, and the initial carbon source and the energy can be provided for the growth of photosynthetic bacterium; Non-growth matrix is a macromolecular substance such as polysaccharide in the starch wastewater, and it can not directly be utilized by photosynthetic bacterium.The interpolation of oxysuccinic acid has strengthened the activity of photosynthetic bacteria cell, has stimulated diastatic generation in the photosynthetic bacteria cell.Under diastatic effect, starch is degraded by photosynthetic bacterium.
3. beneficial effect of the present invention
Of the present inventionly utilizing a strain photosynthetic bacterium to handle starch wastewater and realize the method for sewage recycling, is a kind of sewage water treatment method of easy and simple to handle, economical and efficient.The use of this method can greatly improve sewage treating efficiency, realizes sewage recycling, solves the secondary pollution problem that excess sludge brought.It is that the growth of photosynthetic bacterium provides the initial carbon source and the energy that this method adopts small molecules carbon source oxysuccinic acid, makes bacterial strain directly, degraded starch waste water becomes possibility efficiently.Therefore, this has just omitted the pretreatment section in the photosynthetic bacterium sewage treatment process, has simplified technical process, has overcome the serious problem of existing excess sludge secondary pollution in traditional starch wastewater treatment technology simultaneously; Simultaneously, the thalline rate of increase of this system is 600%, and output is higher.The thalline that produces can directly be used for farming, herd and the starting material of fishery, has purposes widely, and this method can greatly improve the sewage recycling degree.In addition, the by product that oxysuccinic acid is produced as industrial processes is applied in the WWT, and this has just realized making full use of of resource.
Description of drawings
The degradation rate of COD and total reducing sugar and photosynthetic bacterium living weight curve over time in the starch wastewater of Fig. 1 case 1
The degradation rate of COD and total reducing sugar and photosynthetic bacterium living weight curve over time in the starch wastewater of Fig. 2 case 2
Embodiment
Embodiment one: this embodiment is narrated utilizes a strain photosynthetic bacterium to handle starch wastewater and realizes the method for sewage recycling; It is to carry out: the oxysuccinic acid that at first in concentration is the starch wastewater of 8000～30000mg/L, adds 200～600mg/L according to following steps; The pH value of starch wastewater is adjusted to about 7.0～9.0, in starch wastewater, adds the Spherical red antibacterial 160～500mg/L that is in logarithmic phase then.Under the illumination anaerobic condition, handled 120～144 hours, treatment temp is 25～30 ℃.Every in sewage of 24 hours mensuration COD, total reducing sugar and living weight.
Embodiment two: what this embodiment and embodiment one were different is: the starch wastewater concentration of selection is 12000mg/L.Other is identical with embodiment one.
Embodiment three: what this embodiment and embodiment one to two were different is: in starch wastewater, add the oxysuccinic acid of 400mg/L, regulate then about starch wastewater pH value to 7.0.Other is identical with embodiment one.
Oxysuccinic acid is that the growth of photosynthetic bacterium provides the initial carbon source and the energy, and the activity that it has stimulated photosynthetic bacterium has caused the generation of photosynthetic bacterium body endo-amylase, thereby with starch degradation.
Embodiment four: what this embodiment and embodiment one to three were different is: in starch wastewater, add the Spherical red antibacterial 280mg/L that is in logarithmic phase.Other is identical with embodiment one.
Embodiment five: what this embodiment and embodiment one to four were different is: the wastewater treatment condition is the illumination anaerobism, and treatment temp is 25～30 ℃, and the treatment time is 144 hours.The realization of illumination anaerobic condition is carried out through following steps: the incandescent light that adopts 60W is regulated the distance between waste water treatment reactor and the incandescent light as light source, and the maintenance light intensity is 2000～3000lux.The reactor drum mouth is covered with envelope bottle film to guarantee the anaerobic environment of inside reactor.Other is identical with embodiment one.
Embodiment six: what this embodiment and embodiment one to five were different is: whenever measure COD in sewage, total reducing sugar and living weight at a distance from 24h.Other is identical with embodiment one.
Because the staple of starch wastewater is a macromolecular polysaccharide, and the variation tendency of total reducing sugar can characterize the variation tendency of its content, therefore the concentration content through measuring total reducing sugar over time trend can learn the situation that starch wastewater is degraded.
Embodiment:
Case 1
In starch wastewater, add the 400mg/L oxysuccinic acid, regulating starch wastewater pH value is about 7.0, in waste water, adds the Spherical red antibacterial that 280mg/L is in logarithmic phase again, and treatment condition are the illumination anaerobism, and treatment temp is 25～30 ℃, and the treatment time is 144h.COD in sewage of every separated 24h mensuration, total reducing sugar and living weight.
Fig. 1 is the living weight curve over time of degradation rate and the photosynthetic bacterium of COD and total reducing sugar in the starch wastewater of case 1.Wherein X-coordinate is the time, and unit is hour; Left side ordinate zou is a clearance, and unit is a per-cent, and right ordinate zou is a living weight, and unit is mg/L.
Fig. 1 shows: the COD in the starch wastewater and the clearance of total reducing sugar present the trend that rises gradually in time.After 144 hours processing, photosynthetic bacterium reaches 93.2% and 98.9% respectively to the clearance of COD in the starch wastewater and total reducing sugar.This explanation is under metabolism altogether, and photosynthetic bacterium is starch and macromolecular substance in the degradation of sewage effectively.The living weight of photosynthetic bacterium is along with the treatment time presents ascendant trend, and after 144 hours processing, the living weight of photosynthetic bacterium finally reaches 1900mg/L.Be total under the metabolism, small molecules carbon source oxysuccinic acid is that the growth of photosynthetic bacterium provides the carbon source and the energy, and it is active to have improved thalline, has stimulated diastatic generation, thereby has caused photosynthetic bacterium degraded starch waste water.Simultaneously, photosynthetic bacterium utilizes the nutritive substance in the starch wastewater, has realized self growth, and the living weight rate of increase is higher, helps the realization of resource utilization.
Case 2
Present case is the simultaneous test of case 1.Do not add oxysuccinic acid in the starch wastewater, all the other operation stepss and operational condition are with case 1.
Fig. 2 is degradation rate and the photosynthetic bacterium living weight curve over time of COD and total reducing sugar in the starch wastewater of case 2.Wherein X-coordinate is the time, and unit is hour; Left side ordinate zou is a clearance, and unit is a per-cent, and right ordinate zou is a living weight, and unit is mg/L.
Fig. 2 shows: under the situation that does not have the effect of small molecules carbon source, photosynthetic bacterium is degraded starch waste water directly.After 144 hours processing, COD in the starch wastewater and total reducing sugar clearance are respectively 2.7% and 0.2%.Living weight is along with the treatment time presents downward trend, and final thalline output is 126mg/L, be lower than the bacterium amount of initial 280mg/L.This explanation photosynthetic bacterium can not utilize starch wastewater to realize self growth as growth matrix under the situation that altogether metabolism exists, and purifies waste water and the effect of high thalline output thereby reach.
Claims (5)
1. utilize a strain photosynthetic bacterium to handle starch wastewater and realize the method for sewage recycling; The Spherical red antibacterial (Rhodobacter Sphaeroides) that it is characterized in that utilizing a strain to be in logarithmic phase is as the biota of handling starch wastewater; In starch wastewater, add a certain amount of small molecules carbon source oxysuccinic acid; Through after the processing of 144h, the clearance of COD and total reducing sugar can reach more than 90% in the sewage, and the thalline rate of increase is 600%.
2. the method for utilizing strain photosynthetic bacterium processing starch wastewater and realizing sewage recycling according to claim 1; It is characterized in that adopting a strain Spherical red antibacterial to handle starch wastewater; The Spherical red antibacterial that adds belongs to photosynthetic bacterium; It is in logarithmic phase when adding, and dosage is 160mg/L～500mg/L.
3. the method for utilizing strain photosynthetic bacterium processing starch wastewater and realizing sewage recycling according to claim 1; It is characterized in that starch wastewater is main with macromolecular polysaccharide, and contain materials such as protein, resin, starch and microfibre that its carbon source content is abundant; Nontoxic; Be the waste water that can be used as resource utilization matrix, starch wastewater COD content is 8000～30000mg/L, and the pH value is 3.7～4.3.
4. the method for utilizing strain photosynthetic bacterium processing starch wastewater and realizing sewage recycling according to claim 1 is characterized in that in starch wastewater, adding small molecules carbon source oxysuccinic acid, and the concentration that adds is 200～600mg/L.
5. the method for utilizing strain photosynthetic bacterium processing starch wastewater and realizing sewage recycling according to claim 1; It is characterized in that this method can be when disposing of sewage nontoxic, the nutritious in a large number thalline of output, this thalline can directly be reclaimed and as farming, herd, the starting material of industry such as fishing.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107840527A (en) * | 2017-04-21 | 2018-03-27 | 湖南大学 | It is a kind of to utilize dilute floating pulse duckweed and the technique and method of colloid Rhodopseudomonas Combined Treatment organic wastewater |
| CN110904089A (en) * | 2019-12-23 | 2020-03-24 | 清水华明(武汉)生态科技有限公司 | Diversified microorganism system for improving eutrophic water body and application thereof |
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Patent Citations (4)
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| JP2001062494A (en) * | 1999-08-26 | 2001-03-13 | Takaaki Arai | Treatment of waste paper sludge |
| US7442306B2 (en) * | 2003-09-30 | 2008-10-28 | Laurent Olivier | Autotrofic sulfur denitration chamber and calcium reactor |
| CN102432107A (en) * | 2011-08-19 | 2012-05-02 | 上海水平衡环境科技发展有限公司 | Biological integrated purification process for governing black and odorous river channels |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107840527A (en) * | 2017-04-21 | 2018-03-27 | 湖南大学 | It is a kind of to utilize dilute floating pulse duckweed and the technique and method of colloid Rhodopseudomonas Combined Treatment organic wastewater |
| CN107840527B (en) * | 2017-04-21 | 2020-12-15 | 湖南大学 | Method for treating organic wastewater by using lemna paucicostata and pseudomonas glii in combined manner |
| CN110904089A (en) * | 2019-12-23 | 2020-03-24 | 清水华明(武汉)生态科技有限公司 | Diversified microorganism system for improving eutrophic water body and application thereof |
| CN110904089B (en) * | 2019-12-23 | 2020-09-01 | 清水华明(武汉)生态科技有限公司 | Diversified microorganism system for improving eutrophic water body and application thereof |
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Application publication date: 20121003 |