CN104931314A - Stool sample handling method and application thereof - Google Patents
Stool sample handling method and application thereof Download PDFInfo
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- CN104931314A CN104931314A CN201510297661.XA CN201510297661A CN104931314A CN 104931314 A CN104931314 A CN 104931314A CN 201510297661 A CN201510297661 A CN 201510297661A CN 104931314 A CN104931314 A CN 104931314A
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- fecal specimens
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- drying agent
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Abstract
The invention provides a stool sample handling method and application thereof. The stool sample handling method comprises the following steps: placing a stool sample to be detected in a sealable container; adding a drying agent into the container; sealing the container to enable the drying agent to absorb moisture in both the stool sample and air. The stool sample handling method can effectively maintain the stability of detected ingredients in the stool sample, enables self-help sample extraction by patients at home to be possible, and does not hinder downstream detection (immunologic detection); as a stool and drying agent mixture can directly enter the detection process, the flexibility of follow-up stool sample handling process is greatly facilitated, and the method is conductive to detection and treatment of digestive system diseases.
Description
Technical field
The invention belongs to biochemical field, relate to a kind of disposal route and application thereof of fecal specimens.
Background technology
At present, the fecal specimens instability for disease detection is difficult to preserve, therefore the fecal specimens chemical examination of hospital or MEC, must censorship immediately.This causes certain inconvenience to patient, also adds work load to medical personnel, meanwhile, also adds running to testing laboratory and provides the difficulty of result in time.Thus cause patient to coordinate the ratio of sampling to decline, the validity of follow-up relevant medical procedure is also affected.
Although the protection liquid that existing research and development are relevant, technical sophistication, expensive, and transport inconvenience, may leak of liquid be caused.Detection method main at present still detects at hospital's on-site sampling, and Chinese population radix is excessive, and only detecting with hospital is cannot meet our people far away day by day to improve the demand of health examination.Therefore, exploitation is at a low price a kind of, safety, effective fecal specimens antihunt means, is the problem being medically badly in need of at present solving.
Summary of the invention
The present invention is directed to above shortcoming, provide a kind of fecal specimens disposal route, reach cheap safety, effectively can keep the stability of detected composition in fecal specimens, nontoxic or corrosion composition, does not hinder detected downstream, the object of easy-to-operate simultaneously.
For achieving the above object, the present invention takes following technical proposals to realize:
A disposal route for fecal specimens, comprises the steps:
Fecal specimens to be measured is placed in resealable container;
Drying agent is added in container;
By seal of vessel, drying agent absorbs the moisture in fecal specimens and in air.
Further, the water cut in dry rear fecal specimens is less than 10%.
Further, described drying agent is silicon dioxide or activated alumina.
Preferably, drying agent is activated alumina.
Further, the particle diameter of activated alumina is less than 10mm.
Preferably, the particle diameter of activated alumina is 1-2mm.
Further, the consumption of activated alumina is 1.5-3 times of fecal specimens weight.
Further, the quality of the fecal specimens of inserting with can the volume ratio of closed container be 0.05-0.15g/mL.
The present invention also provides the disposal route of above-mentioned fecal specimens detecting the application in disease of digestive system.Fecal specimens disposal route of the present invention can be used for detecting protein, the nucleic acid equal samples in fecal specimens, thus may be used for detecting all kinds of disease of digestive system, comprises hemorrhage of gastrointestinal tract, all kinds of malignant tumour as cancer of the stomach, colorectal cancer etc.
Further, the disposal route that the invention provides above-mentioned fecal specimens is detecting the application in colorectal cancer.
In a word, beneficial effect of the present invention is:
The present invention makes fecal specimens dewater by the method for adding drying agent, as silicon dioxide, and activated alumina etc.The drying agent added is in together in a closed space with fecal specimens, and drying agent directly can absorb the moisture in sample, the moisture also in Absorbable rod air, finally makes sample be in the state of drying and dehydrating thus reach stable effect.
The amount reproduction of bacterium can produce a large amount of metabolic wastes, and decomposites various enzyme, the main cause of the above instability of fecal specimens just, and under the dry condition, the growth and breeding of bacterium is in holddown; Various enzyme, as proteinase, the detected material that DNA enzymatic can directly be degraded in sample, but all enzymes only have and just have activity in a liquid state, and during drying condition, the activity of enzyme almost completely loses; Tested composition in ight soil is with protein, and DNA, RNA are main, and these compositions are unstable in liquid condition, but have good stability in the solid state, and the method for drying can make above material remain on solid state.
Fecal specimens disposal route of the present invention is simple to operate, and effectively can keep the stability of detected composition in fecal specimens, make patient at home self-service sample extraction become possibility, this ight soil stabilizing solution can not hinder detected downstream (immunology detection), the potpourri of ight soil and drying agent directly can enter testing process, be very easy to the follow-up processing flow dirigibility of fecal specimens, contribute to the test-and-treat of disease of digestive system.
Drying agent used in the present invention is conventional material in life at ordinary times, is different from other biological goods, to the mankind without any potential harm, has environmental protection, the advantage of use safety.
High-tech product utilization rate in the elderly and the not high crowd of educational level is not high, and mainly due to its instructions reconditeness, operation is too complicated.Though Related product of the present invention is high-tech product, illustrates and use very simple, being easy to as the elderly and the relative not high crowd of educational level are accepted.The malignant tumours such as colorectal cancer are very high in the incidence of elderly population, some hotspot, and as Haining reaches 6 more than ﹪, the present invention can well improve the extremely long-pending property that the elderly detects intestinal cancer.
In a word, the cheap safety of disposal route of the present invention, effectively can keep the stability of detected composition in fecal specimens, nontoxic or corrosion composition, does not hinder detected downstream, easy-to-operate simultaneously; Can be used for the detection of all kinds of digestive disease, particularly the detection of the malignant tumour such as cancer of the stomach, colorectal cancer.
Further, drying agent composition used in the present invention is cheap, and for activated alumina, per kilogram only 8 yuan, calculate with every part of amount of samples 12g, cost is 0.1 yuan of per person.A lot of economy backwardness relatively at present, most of people in medical condition wretched insufficiency area be both without access to the service of hospital, also cannot bear expensive biological technology products.The present invention reduces production cost greatly, effectively cheap, and the ordinary consumer in these places can be made to be able to enjoy disease detection examination service at a low price.
Further, the activated alumina particle diameter that the present invention uses is less, and its dispersiveness is better, and rate of drying is also faster, is conducive to the decomposition reducing target to be measured in fecal specimens.
Embodiment
Describe the present invention below in conjunction with specific embodiment.
Embodiment 1:
Two groups of experiments are set, pipe 1 and pipe 2 are can closed container, wherein add 5g fecal specimens respectively in pipe 1 and pipe 2, (described fecal specimens is detected as feminine gender through immunogold assay Test paper (FOB)), then adds 100ug/ml human hemoglobin respectively in two pipes.
12g activated alumina desiccant (wherein the diameter of activated alumina is 1mm-2mm) is added again, then closed tube in pipe 1; Pipe 2 in contrast, does not add drying agent closed tube.Above sample is stored in 25 degrees Celsius, is FOB and detects, record the minimum hemoglobin concentration that can detect respectively in the the the the 0th, 1,2,3,6,10 day.
Detection method: get sample in pipe and carry out gradient dilution, record maximum dilution multiple, removes original hemoglobin concentration 100ug/ml by this number, draws after preserving, can detect hemoglobin concentration minimum in ight soil.In experiment, the testing result of the 0th day is 0.2ug/ml, and this is the Monitoring lower-cut of FOB test paper.Ight soil, after preserving, compares the change that can detect minimum haemoglobin in fecal specimens, can know whether haemoglobin is stablized in ight soil.
Testing result is as shown in table 1.
The minimum hemoglobin concentration that the FOB detection method that lower sample preserved by 25 degrees Celsius, table 1 can detect
| Time | 0 day | 1 day | 2 days | 3 days | 6 days | 10 days |
| Pipe 1 | 0.2ug/ml | 0.4ug/ml | 0.4ug/ml | 0.4ug/ml | 0.4ug/ml | 1ug/ml |
| Pipe 2 | 0.2ug/ml | 0.5ug/ml | 2ug/ml | 20ug/ml | Cannot detect |
As shown in table 1, adopt disposal route of the present invention, add the pipe 1 of activated alumina desiccant, in 6 days, the minimum hemoglobin concentration that can detect is 0.4ug/ml, is 1ug/ml, therefore at least 10 days to the 10th day, the equal stable existence of haemoglobin in fecal specimens in pipe 1, can detect.And pipe 2 in contrast, namely degraded at the 1st day, by the 2nd day, minimum hemoglobin concentration just became initial 10 times, within the 6th day, namely cannot detect completely.
Embodiment 2
Arrange two groups of experiments, pipe 1 and pipe 2 are can closed container, and wherein add 5g fecal specimens respectively in pipe 1 and pipe 2, (described fecal specimens is detected as feminine gender through FOB), then adds 100ug/ml human hemoglobin respectively in two pipes.
12g activated alumina desiccant (wherein the diameter of activated alumina is 1mm-2mm) is added again, then closed tube in pipe 1; Pipe 2 in contrast, does not add drying agent closed tube.Above sample is stored in 37 degrees Celsius, is FOB and detects, record the minimum hemoglobin concentration that can detect respectively in the the the the 0th, 1,2,3,6,10 day.
Detection method: get sample in pipe and carry out gradient dilution, record maximum dilution multiple, removes original hemoglobin concentration 100ug/ml by this number, draws after preserving, can detect hemoglobin concentration minimum in ight soil.In experiment, the testing result of the 0th day is 0.2ug/ml, and this is the Monitoring lower-cut of FOB test paper.Ight soil, after preserving, compares the change that can detect minimum haemoglobin in fecal specimens, can know whether haemoglobin is stablized in ight soil.
Testing result is as shown in table 2.
The minimum hemoglobin concentration that the FOB detection method that lower sample preserved by 37 degrees Celsius, table 2 can detect
| Time | 0 day | 1 day | 2 days | 3 days | 6 days | 10 days |
| Pipe 1 | 0.2ug/ml | 0.5ug/ml | 0.5ug/ml | 0.5ug/ml | 1ug/ml | 2ug/ml |
| Pipe 2 | 0.2ug/ml | 1ug/ml | 10ug/ml | Cannot detect |
As shown in table 2, under hot conditions, the decomposition rate of sample is faster.And adopt disposal route of the present invention, add the pipe 1 of activated alumina desiccant, in 3 days, the minimum hemoglobin concentration that can detect is 0.5ug/ml; By the 6th day, be 1ug/ml.Therefore, at least 6 days, the equal stable existence of the haemoglobin in the fecal specimens in pipe 1, can detect.And pipe 2 in contrast, namely degraded at the 1st day, by the 2nd day, minimum hemoglobin concentration just became initial 50 times, within the 3rd day, namely cannot detect completely.
And in practice, the blood content of the fecal occult blood positive just has clinical medicine meaning than GPS survey decades of times, as long as so the reduction of detection sensitivity is no more than 10 times, just there is practical value.According to the express delivery speed of China, the sample of national most area can arrive in 6 days, the data in above-described embodiment 1 and embodiment 2, and it is effective that the disposal route of fecal specimens of the present invention preserves sample.In addition, when seasoning preserves sample, the instability of sample mainly occurs in 24 hours after sampling, and sample is now also fully dry, and part checking matter is in now degrading.And the stability in sample later stage is relatively high, this error produced for the different haulage time of minimizing is very favorable.
Application example:
Coordinate the intestinal cancer people at highest risk of Hangzhou hospital to more than 50 years old, rural area, Zhejiang to carry out mirror to look into, apply method of the present invention, preserve the fecal specimens of examinee, and carry out FOB detection.The result of FOB, as one of evaluating, analyzes the risk that examinee suffers from intestinal cancer.Concrete grammar is as follows:
50ml can close in stopple coupon and add about 12g active oxygen assumed name aluminium drying agent, and with a sample spoon, examinee gets scoop sample product (about 5g) to stopple coupon, then returns, carries out FOB detection.In actual flow process, carry out FOB be approximately 4-7 days detection time from being sampled to return, period sample be in normal temperature.After tester receives sample, add water to 30ml, fill part mixing sample dissolution, carry out colloidal gold method FOB detection, and read signal power, contrast standard product, calculate the content of hemoglobin in tested ight soil.
Key parameter disposal route during concrete calculating is as follows: after (1) adds water to 30ml, because raw manure is 5g, diluted 6 times of calculating by sample; (2) after sampling, sample needs just can detect for 4-7 days, according to existing experimental studies results, sample (sampling latter 24 hours) when early stage, drying was abundant has Partial digestion, and sensitivity can reduce by 2 times, so when calculating rectification, baseline results is multiplied by 2.
It is below the partial data of testing result
The FOB data that table 3 intestinal cancer people at highest risk fecal specimens detects
| Numbering | Raw manure hemoglobin concentration ug/ml |
| 9600 | 0.288 |
| 9609 | 0.294 |
| 2934 | 0.51 |
| 9746 | 0.576 |
| 9730 | 0.78 |
| 9586 | 1.164 |
| 9578 | 2.76 |
| 9603 | 2.85 |
| 2929 | 3.408 |
| 9745 | 4.098 |
| 2704 | 4.368 |
| 9561 | 4.506 |
| 9556 | 4.866 |
| 9623 | 7.35 |
| 9744 | 7.356 |
| 9748 | 7.812 |
| 9702 | 7.878 |
| 2941 | 7.908 |
| 9528 | 8.166 |
| 9708 | 8.652 |
| 9694 | 8.748 |
| 9553 | 8.916 |
| 9640 | 8.982 |
| 9636 | 12.33 |
| 9705 | 16.146 |
| 2844 | 16.32 |
| 9554 | Negative |
| 9594 | Negative |
| 9632 | Negative |
| 9719 | Negative |
| 9610 | Negative |
| 9624 | Negative |
| 9631 | Negative |
| 9606 | Negative |
| 9680 | Negative |
| 9574 | Negative |
| 9634 | Negative |
| 9658 | Negative |
| 9692 | Negative |
| 2620 | Negative |
| 9633 | Negative |
| 2943 | Negative |
| 2721 | Negative |
| 9601 | Negative |
| 9657 | Negative |
| 9643 | Negative |
| 9555 | Negative |
| 9630 | Negative |
| 9654 | Negative |
| 9566 | Negative |
| 9604 | Negative |
| 2932 | Negative |
| 9665 | Negative |
| 9686 | Negative |
| 9596 | Negative |
Have detected 55 increment product in this example altogether, wherein 26 parts of FOB test positive, 29 parts is negative, positive rate close to half, higher than the conventional FOB detection sensitivity of hospital.Because this experiment is for intestinal cancer people at highest risk, FOB Positive rate height is normal phenomenon, and positive rate illustrates that sample treatment respectively of the present invention is effective simultaneously.
Although the present invention with preferred embodiment openly as above; but it is not for limiting the present invention; any those skilled in the art without departing from the spirit and scope of the present invention; the Method and Technology content of above-mentioned announcement can be utilized to make possible variation and amendment to technical solution of the present invention; therefore; every content not departing from technical solution of the present invention; the any simple modification made above embodiment according to technical spirit of the present invention, equivalent variations and modification, all belong to the protection domain of technical solution of the present invention.
Claims (9)
1. a disposal route for fecal specimens, is characterized in that, described method comprises the steps:
Fecal specimens to be measured is placed in resealable container;
Drying agent is added in container;
By seal of vessel, drying agent absorbs the moisture in fecal specimens and in air.
2. disposal route according to claim 1, is characterized in that, the water cut after dry in fecal specimens is less than 10%.
3. disposal route according to claim 1, is characterized in that, described drying agent is silicon dioxide or activated alumina.
4. disposal route according to claim 3, is characterized in that, drying agent is activated alumina.
5. disposal route according to claim 3, is characterized in that, the particle diameter of activated alumina is less than 10mm.
6. disposal route according to claim 4, is characterized in that, the consumption of activated alumina is 1.5-3 times of fecal specimens weight.
7. disposal route according to claim 1, is characterized in that, the quality of the fecal specimens of inserting with can the volume ratio of closed container be 0.05-0.15g/mL.
8. the disposal route of fecal specimens as claimed in claim 1 is detecting the application in disease of digestive system.
9. the disposal route of fecal specimens as claimed in claim 1 is detecting the application in colorectal cancer.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106501045A (en) * | 2016-12-02 | 2017-03-15 | 刘鹏飞 | A kind of fecal specimens processing method and its application |
| CN106769161A (en) * | 2017-01-18 | 2017-05-31 | 杭州诺辉健康科技有限公司 | Quantitative fecal is sampled and pretreatment unit |
| CN111886331A (en) * | 2018-03-15 | 2020-11-03 | 株式会社迈代新 | Method for preserving excrement |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6299842B1 (en) * | 1999-03-05 | 2001-10-09 | Meridian Bioscience, Inc. | Biological sampling and storage container utilizing a desiccant |
| JP2008256389A (en) * | 2007-04-02 | 2008-10-23 | Olympus Corp | Fecal matter sample subjected to drying treatment, preparation method of fecal matter sample, and method for extracting and detecting nucleic acid or protein using fecal matter sample |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6299842B1 (en) * | 1999-03-05 | 2001-10-09 | Meridian Bioscience, Inc. | Biological sampling and storage container utilizing a desiccant |
| JP2008256389A (en) * | 2007-04-02 | 2008-10-23 | Olympus Corp | Fecal matter sample subjected to drying treatment, preparation method of fecal matter sample, and method for extracting and detecting nucleic acid or protein using fecal matter sample |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106501045A (en) * | 2016-12-02 | 2017-03-15 | 刘鹏飞 | A kind of fecal specimens processing method and its application |
| CN106769161A (en) * | 2017-01-18 | 2017-05-31 | 杭州诺辉健康科技有限公司 | Quantitative fecal is sampled and pretreatment unit |
| CN111886331A (en) * | 2018-03-15 | 2020-11-03 | 株式会社迈代新 | Method for preserving excrement |
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