CN1182538A

CN1182538A - Method for extracting disfection biocide from hawthorn nut

Info

Publication number: CN1182538A
Application number: CN 96120686
Authority: CN
Inventors: 韩林
Original assignee: Individual
Current assignee: Individual
Priority date: 1996-11-20
Filing date: 1996-11-20
Publication date: 1998-05-27
Anticipated expiration: 2016-11-20
Also published as: CN1059308C

Abstract

The characteristic lies in that wash the hawthorn seeds and drip their surfacial water, then the dry distillation is carried out to obtain liquid, the semiproduct is separated out through precipitation separation, then the final product liquid is obtained through multiple rectification. Then it is decolored by active carbon to become into slight yellow or colorless transparent water solution product. Said method is simple. The product possesses of quite strong antibiotic activity and is suitable for disinfecting the external skin.

Description

From Chinese hawthorn seed, extract the method for disinfectant

The present invention relates to a kind of method of from Chinese hawthorn seed, extracting disinfectant.

Hawthorn comprises hawthorn, is the little section of rose plant, its pulp has abundant nutrition and medicinal component, and the Chinese hawthorn seed that separates abandons as a kind of waste material, and actual Chinese hawthorn seed is used for the treatment of diseases such as dyspepsia hernia difficult labour, still is not reported and be used to extract disinfection solution.

Purpose of the present invention provides a kind of method of extracting disinfectant for the medical value that develops Chinese hawthorn seed just, thereby not only turns waste into wealth, and also is a kind of natural plants disinfectant of human development.

The objective of the invention is to realize by following technical proposal:

Extract the method for disinfectant from Chinese hawthorn seed, it is characterized in that it is made up of the following step:

A) Chinese hawthorn seed of purchase is packed into after cleaning draining in the destructive distillation container, be heated to 90-110 ℃ and carry out destructive distillation, liquid is collected in the steam condensation;

B) improve heating-up temperature to 200-300 ℃ when no liquid flows out the back, be incubated 3-4 hour, continue destructive distillation, liquid is collected in the steam condensation, flows out until no liquid, stops to heat, and the tail of slagging tap of lowering the temperature is abandoned;

C) with two liquid mixed precipitations of (a) and (b) 40-60 hour, separate the watery thing of upper strata purplish red, be semi-finished product, separate with the glossiness oily thickness of bottom black body;

D) will add thermal distillation in (C) semi-finished product adding alembic again, the rectifying more than at least six sections, rectification temperature is 90-98 ℃, steam is condensed into the liquid tail and abandons, and collects yellow no profit solution and is finished fluid;

E) finished fluid adds active carbon and decolours, and its proportioning is that finished fluid is with the active carbon ratio: 30: 1-2;

F) examination and test of products, the packing warehouse-in.

The method of inspection: in product liquid, add isopyknic ethanol, mix with isopyknic dilute sodium hydroxide again, splash into liquor ferri trichloridi, be the ember precipitation immediately, be blue look after the placement.

The method of inspection: in finished fluid, splash into bromine water, produce white precipitate.

Use above-mentioned extracting method, the disinfectant that makes is measured by analysis and is mainly contained phenol, ursolic acid, oleanolic acid, fumarase, succinic acid etc. have the component of sterilizing function, calculates through chemical normalization method, and wherein each component content is:

Phenol 20-25%, ursolic acid 1-3%, oleanolic acid 2-4%, fumarase 2-3%, succinic acid 2-3%, hydroquinones 10-15%, 35-dimethyl-1H-pyrazoles 25-30%, 2 furan carboxyaldehyde 20-25%, water 0.01-0.02%, other 1-3%, pH value 2-3, proportion 0.988-1.100g/ml.

The product that this method is extracted carries out the antibacterial activity in vitro test through Beijing's Pharmaceutical Analysis research institute, and its result is as follows:

This product is named and is disinfectant, by different matched proportion densities divide five samples respectively dilute five times with the contrast of blank solution, test strain is: 1. staphylococcus aureus, 2. pseudomonas aeruginosa, 3. Escherichia coli are all available from Nat'l Pharmaceutical ﹠ Biological Products Control Institute.

Medium: 1. plain agar slant medium, 2. broth medium, 3. II PH6.5 medium is three kinds.

Test method:

Above-mentioned 3 kinds of bacterial strains are got a platinum loop respectively on the lawn on the plain agar slant medium, be inoculated in the 4ml broth medium, put in 37 ℃ of incubators and cultivated 18-24 hour, take out standby.

It is an amount of to get above-mentioned 3 kinds of bacterium liquid, is heated to respectively to make in the II PH6.5 medium under suitable temperature to contain bacteria concentration and be 1% the medium that carries disease germs, and shakes up, standby.

Get 12 pairs in aseptic pair of dish, 4 pairs of every kind of test organismss inject the above-mentioned medium 20ml that carries disease germs respectively and shake up under suitable temperature and half aseptic condition, treat culture medium solidifying after, put the steel pipe point sample, put in 37 ℃ of incubators and cultivated 16-18 hour, take out, measure antibacterial circle diameter.

Result of the test sees Table 1

Table 1

Number antibacterial 12345 antibacterial activities comparison diameter (mm) strain golden look staphylococcus 22.38 26.46 22.29 19.02 14.46 2＞1-3＞4＞5 green amine bacillus 17.22 11.76 15.92 15.30 10.03 1＞3＞4＞2＞5 Escherichia coli 21.46 16.93 21.97 21.38 16.01 1=3=4＞2＞5 results and show numbering 1,2,3,4,5 the test agent solution that supplies all has bigger inhibition zone to occur, stronger antibacterial activity is promptly arranged, the blank solution of numbering 6 does not have inhibition zone and occurs, promptly there is not antibiotic activity, from the solution of five kinds of different matched proportion densities to staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli all have stronger antibacterial activity.

The disinfectant that method of the present invention is extracted is applicable to the sterilization of human body skin and thing table and air, sterilization.

The technology of the present invention has following advantage and effect:

A) extracting method is simple, and product has sterilizing function, belongs to the pure natural plant extract, no side effects;

B) the product consumption is few, and effect is remarkable.

C) raw material sources are wide, and twice laid, turn waste into wealth.

Embodiment 1:

80kg Chinese hawthorn seed with purchase, water cleans the back draining, pack into and be heated to 90 ℃ ± 5 in the destructive distillation jar of 100 liters, carry out destructive distillation, the steam that produces is collected liquid after condensation, improve temperature 300 ℃ ± 5 again, is incubated after 3 hours, continue destructive distillation and obtain liquid, twice liquid merges back quiescent setting 60 hours, tells the watery thing semi-finished product of upper strata purplish red 5kg, the glossiness oily thickness of the black at the bottom of knockout drum body, with semi-finished product rectifying, it is finished product 3.5kg that 90-92 ℃ of its temperature, its steam are carried out eight sections yellow no profit solution bodies of rectifying condensations collection, adds active carbon 120g, the decolouring back becomes product, check bottling packing.

Embodiment 2:

90kg Chinese hawthorn seed with purchase, water cleans the back draining, pack into and be heated to 100 ℃ ± 5 in the destructive distillation jar of 100 liters, carry out destructive distillation, the steam that produces is collected liquid after condensation, improve temperature 250 ℃ ± 5 again, is incubated after 3.5 hours, continue destructive distillation and obtain liquid, twice liquid merges back quiescent setting 50 hours, tells the watery thing semi-finished product of upper strata purplish red 5.6kg, the glossiness oily thickness of the black at the bottom of knockout drum body, with semi-finished product rectifying, it is finished product 3.92kg that 92-94 ℃ of its temperature, its steam are carried out seven sections yellow no profit solution bodies of rectifying condensations collection, adds active carbon 260g, the decolouring back becomes product, check bottling packing.

Embodiment 3:

80kg Chinese hawthorn seed with purchase, water cleans the back draining, pack into and be heated to 110 ℃ ± 5 in the destructive distillation jar of 100 liters, carry out destructive distillation, the steam that produces is collected liquid after condensation, improve temperature 200 ℃ ± 5 again, is incubated after 4 hours, continue destructive distillation and obtain liquid, twice liquid merges back quiescent setting 40 hours, tells the watery thing semi-finished product of upper strata purplish red 5kg, the glossiness oily thickness of the black at the bottom of knockout drum body, with semi-finished product rectifying, it is finished product 3.6kg that 96-98 ℃ of its temperature, its steam are carried out six sections yellow no profit solution bodies of rectifying condensations collection, adds active carbon 125g, the decolouring back becomes product, check bottling packing.

Claims

1, extract the method for disinfectant from Chinese hawthorn seed, it is characterized in that it is made up of the following step:

D) will add thermal distillation in (C) semi-finished product adding alembic again, the rectifying more than at least six sections,, rectification temperature is 90-98 ℃, steam is condensed into the liquid tail and abandons, and collects yellow no profit solution and is finished fluid;

F) examination and test of products, the packing warehouse-in.

2, preparation method according to claim 1 is characterized in that the method for inspection: add isopyknic ethanol in product liquid, mix with isopyknic dilute sodium hydroxide, splash into liquor ferri trichloridi, be the ember precipitation immediately, be blue look after the placement.

3, preparation method according to claim 1 is characterized in that the method for inspection: splash into bromine water in finished fluid, produce white precipitate.