CN1505685A - 快速检测和鉴定生物物体的方法 - Google Patents
快速检测和鉴定生物物体的方法 Download PDFInfo
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Images
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Abstract
Description
质量标志 | 双链序列 | 单链序列 | 此链的总质量 | 此链的碱基信息 | 另一条链的碱基信息 | 上部链的总碱基组成 | 底部链的总碱基组或 |
T*Δ质量(T*-T)=x | T*ACGT*ACGT*AT*GCAT*GCA | T*ACGT*ACGT* | 3x | 3T | 3A | 3T2A2C2G | 3A2T2G2C |
AT*GCAT*GCA | 2x | 2T | 2A | ||||
C*Δ质量(C*-C)=y | TAC*GTAC*GTATGC*ATGC*A | TAC*GTAC*GT | 2x | 2C | 2G | ||
ATGC*ATGC*A | 2x | 2C | 2G |
引物区域 | 16S_971 | 16S_1100 | 16S_1337 | 16S_1294 | 16S_1228 | 23S_1021 | 23S_855 | 23S_193 | 23S_115 |
病菌名字 | |||||||||
醋酸钙不动杆菌 | 55619.1 | 55004 | 28446.7 | 35854.9 | 51295.4 | 30299 | 42654 | 39557.5 | 54999 |
炭疽芽胞杆菌 | 55005 | 54388 | 28448 | 35238 | 51296 | 30295 | 42651 | 39560 | 56850 |
蜡样芽胞杆菌 | 55622.1 | 54387.9 | 28447.6 | 35854.9 | 51296.4 | 30295 | 42651 | 39560.5 | 55850.3 |
支气管败血性博代杆菌 | 56857.3 | 51300.4 | 28446.7 | 35857.9 | 51307.4 | 30299 | 42653 | 39559.5 | 51920.5 |
疏螺旋体burgdorferi | 56231.2 | 55621.1 | 28440.7 | 35852.9 | 51295.4 | 30297 | 42029.9 | 38941.4 | 52524.6 |
流产布鲁菌 | 58098 | 55011 | 28448 | 35854 | 50683 | ||||
弯曲菌jejuni | 58068.5 | 54386.9 | 29061.8 | 35856.9 | 50674.3 | 30294 | 420322.9 | 39558.5 | 45732.5 |
肺炎衣原体 | 55000 | 55007 | 29063 | 35855 | 50676 | 30295 | 42036 | 38941 | 56230 |
肉毒梭菌 | 55006 | 53767 | 28445 | 35855 | 51291 | 30800 | 42656 | 39562 | 54999 |
艰难梭菌 | 56855.3 | 54386.9 | 28447.7 | 35853.9 | 51296.4 | 30294 | 41417.8 | 39556.5 | 55612.2 |
粪肠球菌 | 55620.1 | 54387.9 | 28447.6 | 35858.9 | 51296.4 | 30297 | 42652 | 39559.5 | 56849.3 |
大肠杆菌 | 55622 | 55009 | 28445 | 35857 | 51301 | 30301 | 42656 | 39562 | 54999 |
土接热弗朗西丝菌 | 53769 | 54385 | 28445 | 35856 | 51298 | ||||
流感嗜血杆菌 | 55620.1 | 55006 | 28444.7 | 35855.9 | 51298.4 | 30298 | 42656 | 39560.5 | 55613.1 |
肺炎克氏杆菌 | 556221 | 55008 | 28442.7 | 35856.9 | 51297.4 | 30300 | 42656 | 39562 5 | 55000 |
嗜肺军团菌 | 55618 | 55626 | 28446 | 35857 | 51303 | ||||
禽分枝杆菌 | 54390.9 | 55631.1 | 29064.8 | 35858.9 | 51915.5 | 30298 | 42656 | 389424 | 56241.2 |
麻风分枝杆菌 | 54389.9 | 55629.1 | 29064.8 | 35860.9 | 51917.5 | 30298 | 42656 | 39559.5 | 56240.2 |
结核分枝杆菌 | 54390.9 | 55629.1 | 29064.8 | 35860.9 | 51301.4 | 30299 | 42656 | 39560.5 | 56243.2 |
生殖道支原体 | 53143.7 | 45115.4 | 29061.8 | 35854.9 | 50671.3 | 30294 | 43264.1 | 39558.5 | 56842.4 |
肺炎支原体 | 53143.7 | 45118.4 | 29061.8 | 35854.9 | 50673.3 | 30294 | 43264.1 | 39559.5 | 56843.4 |
奈瑟淋球菌 | 55627.1 | 54389.9 | 28445.7 | 35855.9 | 51302.4 | 30300 | 42649 | 39561.5 | 55000 |
绿脓假单胞菌 | 55623 | 55010 | 28443 | 35858 | 51301 | 30298 | 43272 | 39558 | 55619 |
普氏立克次体 | 58093 | 55621 | 28448 | 35853 | 50677 | 30293 | 42650 | 39559 | 53139 |
立氏立克次体 | 58094 | 55623 | 28448 | 35853 | 50679 | 30293 | 42648 | 39559 | 53755 |
鼠伤寒沙门杆菌 | 55622 | 55005 | 28445 | 35857 | 51301 | 30301 | 42658 | ||
志贺痢疾菌 | 55623 | 55009 | 28444 | 35857 | 51301 | ||||
金黄色葡萄球菌 | 56854.3 | 54386.9 | 28443.7 | 358529 | 51294.4 | 30298 | 42655 | 39559.5 | 57466.4 |
链霉菌 | 54389.9 | 59341.6 | 29063.8 | 35858.9 | 51300.4 | 39563.5 | 56864.3 | ||
梅毒密螺旋体 | 56245.2 | 55631.1 | 28445.7 | 35851.9 | 51297.4 | 30299 | 42034.9 | 38939.4 | 57473.4 |
霍乱弧菌 | 55625 | 55626 | 28443 | 35857 | 52536 | 29063 | 30303 | 35241 | 50675 |
副溶血性弧菌 | 54384.9 | 55626.1 | 28444.7 | 34620.7 | 50064.2 | ||||
耶尔森鼠疫杆菌 | 55620 | 55626 | 28443 | 35857 | 51299 |
计算的质量 | 误差 | 碱基组成 |
14208.2935 | 0.079520 | A1 G17 C10 T18 |
14208.3160 | 0.056980 | A1 G20 C15 T10 |
14208.3386 | 0.034440 | A1 G23 C20 T2 |
14208.3074 | 0.065560 | A6 G11 C3 T26 |
14208.3300 | 0.043020 | A6 G14 C8 T18 |
14208.3525 | 0.020480 | A6 G17 C13 T10 |
14208.3751 | 0.002060 | A6 G20 C18 T2 |
14208.3439 | 0.029060 | A11 G8 C1 T26 |
14208.3665 | 0.006520 | A11 G11 C6 T18 |
14208.3890 | 0.016020 | A11 G14 C11 T10 |
14208.4116 | 0.038560 | A11 G17 C16 T2 |
14208.4030 | 0.029980 | A16 G8 C4 T18 |
14208.4255 | 0.052520 | A16 G11 C9 T10 |
14208.4481 | 0.075060 | A16 G14 C14 T2 |
14208.4395 | 0.066480 | A21 G5 C2 T18 |
14208.4620 | 0.089020 | A21 G8 C7 T10 |
14079.2624 | 0.080600 | A0 G14 C13 T19 |
14079.2849 | 0.058060 | A0 G17 C18 T11 |
14079.3075 | 0.035520 | A0 G20 C23 T3 |
14079.2538 | 0.089180 | A5 G5 C1 T35 |
14079.2764 | 0.066640 | A5 G8 C6 T27 |
14079.2989 | 0.044100 | A5 G11 C11 T19 |
14079.3214 | 0.021560 | A5 G14 C16 T11 |
14079.3440 | 0.000980 | A5 G17 C21 T3 |
14079.3129 | 0.030140 | A10 G5 C4 T27 |
14079.3354 | 0.007600 | A10 G8 C9 T19 |
14079.3579 | 0.014940 | A10 G11 C14 T11 |
14079.3805 | 0.037480 | A10 G14 C19 T3 |
14079.3494 | 0.006360 | A15 G2 C2 T27 |
14079.3719 | 0.028900 | A15 G5 C7 T19 |
14079.3944 | 0.051440 | A15 G8 C12 T11 |
14079.4170 | 0.073980 | A15 G11 C17 T3 |
14079.4084 | 0.065400 | A20 G2 C5 T19 |
14079.4309 | 0.087940 | A20 G5 C10 T13 |
生物体名字 | 23S_855 | 16S_1337 | 23S_1021 |
炭疽杆菌 | 42650.98 | 28447.65 | 30294.98 |
金黄色葡萄球菌 | 42654.97 | 28443.67 | 30297.96 |
生物体名字 | 16S_971 | 16S_1294 | 16S_1228 |
霍乱弧菌 | 55625.09 | 35856.87 | 52535.59 |
副溶血性弧菌 | 54384.91 | 34620.67 | 50064.19 |
区域 | 生物体名字 | 长度 | 分子量 | 碱基组成 |
16S_1100-1188 | 分枝杆菌avium | 82 | 25624.1728 | A16G32C18T16 |
16S_1100-1188 | 链霉菌sp | 96 | 29904.871 | A17G38C27T14 |
生物体名字 | 碱基组成 | 生物体名字 | 碱基组成 |
禽分枝杆菌 | A16G32C18T16 | 霍乱弧菌 | A23G30C21T16 |
链霉菌sp | A17G38C27T14 | 嗜水气单胞菌 | A23G31C21T15 |
脲原体 | A18G30C17T17 | 杀鲑气单胞菌 | A23G31C21T15 |
链霉菌sp | A19G36C24T18 | 生殖道支原体 | A24G19C12T18 |
麻风分枝杆菌 | A20G32C22T16 | 肉毒梭菌 | A24G25C18T20 |
结核分枝杆菌 | A20G33C21T16 | 支气管炎博代杆菌 | A24G26C19T14 |
星状诺卡菌 | A20G33C21T16 | 土拉热弗朗西丝菌 | A24G26C19T19 |
杯死梭杆菌 | A21G26C22T18 | 炭疽杆菌 | A24G26C20T18 |
产单核细胞李斯特菌 | A21G27C19T19 | 弯曲菌jejuni | A24G26C20T18 |
肉毒梭菌 | A21G27C19T21 | 金黄色葡萄球菌 | A24G26C20T18 |
奈瑟淋球菌 | A21G28C21T18 | 幽门螺旋菌 | A24G26C20T19 |
quintana巴尔通体 | A21G30C22T16 | 幽门螺旋菌 | A24G26C21T18 |
粪肠球菌 | A22G27C20T19 | 卡他募拉菌 | A24G26C23T16 |
巨大芽胞杆菌 | A22G28C20T18 | 流感嗜血杆菌 | A24G28C20T17 |
枯草芽胞杆菌 | A22G28C21T17 | 沙眼衣原体 | A24G28C21T16 |
绿脓假单胞菌 | A22G29C23T15 | 肺炎衣原体 | A24G28C21T16 |
嗜肺军团菌 | A22G32C20T16 | 肺炎衣原体AR39 | A24G28C21T16 |
肺炎支原体 | A23G20C14T16 | 恶臭假单胞菌 | A24G29C21T16 |
肉毒梭菌 | A23G26C20T19 | 普通变形杆菌 | A24G30C21T15 |
粪肠球菌 | A23G26C21T18 | 耶尔森鼠疫杆菌 | A24G30C21T15 |
醋酸钙不动杆菌 | A23G26C21T19 | 耶尔森假结核菌 | A24G30C21T15 |
钩端螺旋体borgpeterseni | A23G26C24T15 | 肉毒梭菌 | A25G24C18T21 |
问号钩端螺旋体interrogans | A23G26C24T15 | 破伤风梭菌 | A25G25C18T20 |
产气荚膜梭菌 | A23G27C19T19 | 土拉热弗朗西丝菌 | A25G25C19T19 |
炭疽芽胞杆菌 | A23G27C20T18 | 醋酸钙不动杆菌 | A25G26C20T19 |
蜡样芽胞杆菌 | A23G27C20T18 | 碎弱类杆菌 | A25G27C16T22 |
苏云金芽胞杆菌 | A23G27C20T18 | Chlamydophila psittaci | A25G27C21T16 |
嗜水气单胞菌 | A23G29C21T16 | 疏螺旋体burgdorferi | A25G29C17T19 |
大肠杆菌 | A23G29C21T16 | 念珠形链杆菌 | A26G26C20T16 |
大肠假单胞菌 | A23G29C21T17 | 立克次体prowasekii | A26G28C18T18 |
恶臭杆菌 | A23G29C22T15 | 立氏立克次体 | A26G28C20T16 |
志贺痢疾菌 | A23G29C22T15 | 支原体mycoides | A26G23C16T20 |
生物体 | 16S_971-1062 | 16S_1128-1310 | 16S_1100-1188 |
嗜水气单胞菌 | A21G29C22T20 | A22G27C21T13 | A23G31C21T15 |
杀鲑气单胞菌 | A21G29C22T20 | A22G27C21T13 | A23G31C21T15 |
炭疽杆菌 | A21G27C22T22 | A24G22C19T18 | A23G27C20T18 |
蜡样芽胞杆菌 | A22G27C21T22 | A24G22C19T18 | A23G27C20T18 |
苏去金芽胞菌 | A22G27C21T22 | A24G22C19T18 | A23G27C20T18 |
沙眼衣原体 | A22G26C20T23 | A24G23C19T16 | A24G28C21T16 |
肺炎衣原体AR39 | A26G23C20T22 | A26G22C16T18 | A24G28C21T16 |
钩端螺旋体borgpeterseni | A22G26C20T21 | A22G25C21T15 | A23G26C24T15 |
问号钩端螺旋体 | A22G26C20T21 | A22G25C21T15 | A23G26C24T15 |
生殖道支原体 | A28G23C15T22 | A30G18C15T19 | A24G19C12T18 |
肺炎支原体 | A28G23C15T22 | A27G19C16T20 | A23G20C14T16 |
大肠杆菌 | A22G28C20T22 | A24G25C21T13 | A23G29C22T15 |
志贺痢疾菌 | A22G28C21T21 | A24G25C21T13 | A23G29C22T15 |
普通变形杆菌 | A23G26C22T21 | A26G24C19T14 | A24G30C21T15 |
耶尔森鼠疫杆菌 | A24G25C21T22 | A25G24C20T14 | A24G30C21T15 |
耶尔森假结核菌 | A24G25C21T22 | A25G24C20T14 | A24G30C21T15 |
土接热弗朗西丝菌 | A20G25C21T23 | A23G26C17T17 | A24G26C19T19 |
普氏立克次体 | A21G26C24T25 | A24G23C16T19 | A26G28C18T18 |
立氏立克次体 | A21G26C25T24 | A24G24C17T17 | A26G28C20T16 |
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