CN1846131A - 制备窄的均匀试剂条的方法和试剂 - Google Patents
制备窄的均匀试剂条的方法和试剂 Download PDFInfo
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- CN1846131A CN1846131A CNA2004800239248A CN200480023924A CN1846131A CN 1846131 A CN1846131 A CN 1846131A CN A2004800239248 A CNA2004800239248 A CN A2004800239248A CN 200480023924 A CN200480023924 A CN 200480023924A CN 1846131 A CN1846131 A CN 1846131A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T156/00—Adhesive bonding and miscellaneous chemical manufacture
- Y10T156/10—Methods of surface bonding and/or assembly therefor
- Y10T156/1052—Methods of surface bonding and/or assembly therefor with cutting, punching, tearing or severing
- Y10T156/1059—Splitting sheet lamina in plane intermediate of faces
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T156/00—Adhesive bonding and miscellaneous chemical manufacture
- Y10T156/10—Methods of surface bonding and/or assembly therefor
- Y10T156/1052—Methods of surface bonding and/or assembly therefor with cutting, punching, tearing or severing
- Y10T156/1084—Methods of surface bonding and/or assembly therefor with cutting, punching, tearing or severing of continuous or running length bonded web
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- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
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- Sampling And Sample Adjustment (AREA)
Abstract
Description
分析物 | 酶 | 介体(氧化形式) | 辅助介体 |
葡萄糖 | 葡萄糖脱氢酶和心肌黄酶 | 铁氰化物 | |
葡萄糖 | 葡萄糖脱氢酶(喹啉蛋白质(quinoprotein)) | 铁氰化物 | |
胆固醇 | 胆固醇酯酶和胆固醇氧化酶 | 铁氰化物 | 2,6-二甲基-1,4-苯醌2,5-二氯-1,4-苯醌或者吩嗪乙基硫酸盐(ethosulfate) |
HDL胆固醇 | 胆固醇酯酶和胆固醇氧化酶 | 铁氰化物 | 2,6-二甲基-1,4-苯醌2,5-二氯-1,4-苯醌或者吩嗪乙基硫酸盐(ethosulfate) |
甘油三酸酯 | 脂蛋白脂肪酶、甘油激酶和甘油-3-磷酸酯氧化酶 | 铁氰化物或吩嗪乙基硫酸盐(ethosulfate) | 吩嗪甲基硫酸盐(methosulfate) |
乳酸酯 | 乳酸酯氧化酶 | 铁氰化物 | 2,6-二甲基-1,4-苯醌 |
乳酸酯 | 乳酸酯脱氢酶和心肌黄酶 | 铁氰化物吩嗪乙基硫酸盐(ethosulfate)、或者吩嗪甲基硫酸盐(methosulfate) | |
乳酸酯脱氢酶 | 心肌黄酶 | 铁氰化物 | 吩嗪乙基硫酸盐(ethosulfate)、或者吩嗪甲基硫酸盐(methosulfate) |
丙酮酸酯 | 丙酮酸酯氧化酶 | 铁氰化物 | |
乙醇 | 乙醇氧化酶 | 亚苯基二胺 | |
胆红素 | 胆红素氧化酶 | 1-甲氧基-吩嗪甲基硫酸盐(methosulfate) | |
尿酸 | 尿素酶 | 铁氰化物 |
物质 | 来源 | %w/w |
KeltrolF(黄原胶) | Kelco | 0.2136% |
羧甲基纤维素(CMC) | Hercules-Aqualon | 0.5613% |
聚乙烯吡咯烷酮(PVP)K25 | BASF | 1.8952% |
Propiofan(聚氯乙烯)(50%水) | BASF | 2.8566% |
葡萄糖-染料-氧化还原酶(GlucDOR)(E.C.1.1.99.17) | Roche Diagnostics | 0.3310% |
吡咯并喹啉奎宁(PQQ) | Roche Diagnostics | 0.0092% |
Sipernat320 DS(合成的无定形的沉淀二氧化硅) | Degussa Hüls | 2.0039% |
琥珀酸钠六水合物 | Mallinckrodt Chmeicals | 0.4803% |
海藻糖 | Sigma-Aldrich | 0.4808% |
KH2PO4 | J.T.Baker | 0.4814% |
K2HPO4 | J.T.Baker | 1.1166% |
N,N-二-(羟乙基)-3-甲氧基对亚硝基苯胺 | Roche Diagnostics | 0.6924% |
Mega 8(正辛酰基-N-甲基葡糖酰胺) | Dojindo | 0.2806% |
GeroponT 77(N-甲基N-油基牛磺酸钠) | Rhodia Chimie | 0.0298% |
KOH | Merck | 0.1428% |
水,二重蒸馏 | 89.9558% |
物质 | 来源 | 在试剂中的最终浓度 |
甘氨酸 | Sigma | 23g/l |
聚乙二醇 | Sigma | 23g/l |
蔗糖 | Sigma | 55g/l |
牛血清白蛋白 | Sigma | 6.9g/l |
Mega 8(正辛酰基-N-甲基葡糖酰胺) | Dojindo | 1g/l |
刃天青 | Sigma-Aldrich Chemie GmbH | 1.4g/l5.6mmol/l |
Polybrene(hexadimethrine bromide) | Sigma | 0.015g/l |
Moviol4/86(聚乙烯醇) | Clariant GmbH | 20g/l |
KeltrolF(黄原胶) | Kelco | 2.89g/l |
Electrozym TH(还原的Chromozym TH;还原的甲苯磺酰基-甘氨酰基-脯氨酰基-精氨酸-4-苯基重氮酸乙酸盐 | Roche Diagnostics | 1.226g/l1.9mmol/l |
大豆磷脂 | ||
重组组织因子的溶液 | Dade-Behring | 109μg/l |
物质 | 来源 | %w/w |
KeltrolF(黄原胶) | Kelco | 0.20% |
GantrezS97(甲基乙烯基醚/马来酐共聚物) | ISP | 2.48 |
聚乙烯吡咯烷酮(PVP)K25 | BASF | 1.93% |
Propiofan(聚氯乙烯)(50%水) | BASF | 2.94% |
葡萄糖-染料-氧化还原酶(GlucDOR)(E.C.1.1.99.17?) | Roche Diagnostics | 0.33% |
吡咯并喹啉奎宁(PQQ) | Roche Diagnostics | 0.0093% |
二氧化硅FK 300 DS | Degussa Hüls | 1.77% |
KH2PO4 | J.T.Baker | 0.48% |
K2HPO4 | J.T.Baker | 1.47% |
N,N-二-(羟乙基)-3-甲氧基对亚硝基苯胺 | Roche Diagnostics | 0.69% |
Mega 8(正辛酰基-N-甲基葡糖酰胺) | Dojindo | 0.29% |
GeroponT 77(N-甲基N-油基牛磺酸钠) | Rhodia Chimie | 0.030% |
KOH | Merck | 1.14% |
水,二重蒸馏 | 86.227% |
物质 | 来源 | %w/w |
KeltrolF(黄原胶) | Kelco | 0.20% |
GantrezS97(甲基乙烯基醚/马来酐共聚物) | ISP | 0.50% |
羧甲基纤维素(CMC) | Hercules-Aqualon | 0.50% |
聚乙烯吡咯烷酮(PVP)K25 | BASF | 1.90% |
Propiofan(聚氯乙烯)(50%水) | BASF | 2.89% |
葡萄糖-染料-氧化还原酶(GlucDOR)(E.C.1.1.99.17?) | Roche Diagnostics | 0.34% |
吡咯并喹啉奎宁(PQQ) | Roche Diagnostics | 0.0093% |
KH2PO4 | J.T.Baker | 0.48% |
K2HPO4 | J.T.Baker | 1.46% |
N,N-二-(羟乙基)-3-甲氧基对亚硝基苯胺 | Roche Diagnostics | 0.71% |
Mega 8(正辛酰基-N-甲基葡糖酰胺) | Dojindo | 0.28% |
GeroponT 77(N-甲基N-油基牛磺酸钠) | Rhodia Chimie | 0.030% |
KOH | Merck | 0.31% |
水,二重蒸馏 | 90.384% |
CMC | Keltrol | PVP | Propiofan | 二氧化硅 | 粘度 | 触变性 |
0.56 | 0.21 | 1.9 | 2.86 | 2 | 117 | 是 |
0.476 | 0.28 | 1.52 | 2.29 | 2 | 99 | 是 |
0 | 0.77 | 1.52 | 2.29 | 2 | 69.5 | 是 |
0.77 | 0 | 2.28 | 3.43 | 2 | 149 | 是 |
0.504 | 0.4 | 1.9 | 2.86 | 2 | 123 | 弱 |
Claims (20)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US48039703P | 2003-06-20 | 2003-06-20 | |
US60/480,397 | 2003-06-20 | ||
PCT/US2004/019591 WO2004113917A2 (en) | 2003-06-20 | 2004-06-18 | Method and reagent for producing narrow, homogenous reagent strips |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1846131A true CN1846131A (zh) | 2006-10-11 |
CN1846131B CN1846131B (zh) | 2012-01-18 |
Family
ID=33539293
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2004800239248A Active CN1846131B (zh) | 2003-06-20 | 2004-06-18 | 制备窄的均匀试剂条的方法和试剂 |
CNB2004800238052A Active CN100504369C (zh) | 2003-06-20 | 2004-06-18 | 具有扩口式试样接收室的测试条 |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB2004800238052A Active CN100504369C (zh) | 2003-06-20 | 2004-06-18 | 具有扩口式试样接收室的测试条 |
Country Status (14)
Country | Link |
---|---|
US (8) | US7749437B2 (zh) |
EP (4) | EP1642117B1 (zh) |
JP (7) | JP4800209B2 (zh) |
KR (1) | KR100785670B1 (zh) |
CN (2) | CN1846131B (zh) |
CA (4) | CA2529657C (zh) |
ES (4) | ES2681398T3 (zh) |
HK (1) | HK1094599A1 (zh) |
HU (1) | HUE039852T2 (zh) |
MX (1) | MXPA05013635A (zh) |
PL (4) | PL1639354T3 (zh) |
PT (1) | PT1639352T (zh) |
TR (1) | TR201810169T4 (zh) |
WO (4) | WO2004113902A1 (zh) |
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