DE10011555T1 - Automatisches Diagnoseanalysegerät und Verfahren - Google Patents

Automatisches Diagnoseanalysegerät und Verfahren Download PDF

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Publication number
DE10011555T1
DE10011555T1 DE10011555T DE10011555T DE10011555T1 DE 10011555 T1 DE10011555 T1 DE 10011555T1 DE 10011555 T DE10011555 T DE 10011555T DE 10011555 T DE10011555 T DE 10011555T DE 10011555 T1 DE10011555 T1 DE 10011555T1
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magnet
container
reaction vessel
constructed
reaction
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DE10011555T
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English (en)
Inventor
wird später genannt werden Erfinder
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Gen Probe Inc
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Gen Probe Inc
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Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Publication of DE10011555T1 publication Critical patent/DE10011555T1/de
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    • Y10T436/2575—Volumetric liquid transfer

Abstract

Vorrichtung zum Durchführen eines magnetischen Trennungs-Reinigungs-Verfahrens an einer Lösung, die magnetisch reagierende Partikel enthält und in einem Reaktionsbehälter enthalten ist, wobei die Vorrichtung umfasst:
– eine Behälterträgereinheit, die konstruiert und angeordnet ist, um den Reaktionsbehälter aufzunehmen und lösbar zu halten;
– eine Magnetbewegungsstruktur, die wenigstens einen Magneten umfasst, wobei die Magnetbewegungsstruktur konstruiert und angeordnet ist, um den Magneten zwischen einer ersten Position und einer zweiten Position bezüglich des Reaktionsbehälters zu bewegen, wobei der Magnet die magnetisch reagierenden Partikel zu einer inneren Oberfläche des Reaktionsbehälters, die dem Magneten benachbart ist, zieht, wenn der Magnet in der zweiten Position ist, und wobei die Anziehung der magnetisch reagierenden Partikel zu dem Magneten größer ist, wenn der Magnet in der zweiten Position ist, als wenn der Magnet in der ersten Position ist;
– einen Flüssigkeitstransfermechanismus, der konstruiert und angeordnet ist, um Flüssigkeit in den Reaktionsbehälter abzugeben und Flüssigkeit aus dem...

Claims (15)

  1. Vorrichtung zum Durchführen eines magnetischen Trennungs-Reinigungs-Verfahrens an einer Lösung, die magnetisch reagierende Partikel enthält und in einem Reaktionsbehälter enthalten ist, wobei die Vorrichtung umfasst: – eine Behälterträgereinheit, die konstruiert und angeordnet ist, um den Reaktionsbehälter aufzunehmen und lösbar zu halten; – eine Magnetbewegungsstruktur, die wenigstens einen Magneten umfasst, wobei die Magnetbewegungsstruktur konstruiert und angeordnet ist, um den Magneten zwischen einer ersten Position und einer zweiten Position bezüglich des Reaktionsbehälters zu bewegen, wobei der Magnet die magnetisch reagierenden Partikel zu einer inneren Oberfläche des Reaktionsbehälters, die dem Magneten benachbart ist, zieht, wenn der Magnet in der zweiten Position ist, und wobei die Anziehung der magnetisch reagierenden Partikel zu dem Magneten größer ist, wenn der Magnet in der zweiten Position ist, als wenn der Magnet in der ersten Position ist; – einen Flüssigkeitstransfermechanismus, der konstruiert und angeordnet ist, um Flüssigkeit in den Reaktionsbehälter abzugeben und Flüssigkeit aus dem Reaktionsbehälter zu entnehmen, und – einen Trägerbewegungsmechanismus, der funktionsfähig mit der Behälterträgereinheit verbunden ist, und so konstruiert und angeordnet ist, dass eine zyklische Bewegung auf die Behälterträgereinheit übertragen wird, um die Lösung, die in dem Reaktionsbehälter enthalten ist, zu bewegen und zu mischen.
  2. Vorrichtung nach Anspruch 1, außerdem umfassend ein Gehäuse, das eine innere Kammer definiert, wobei die Behälterträgereinheit, die Magnetbewegungsstruktur, wenigstens ein Teil des Flüssigkeitstransfermechanismus und der Trägerbewegungsmechanismus innerhalb der inneren Kammer angeordnet sind.
  3. Vorrichtung nach Anspruch 1 oder 2, wobei die Magnetbewegungsstruktur konstruiert und angeordnet ist, um sich um einen Drehpunkt und zwischen der ersten und der zweiten Position zu drehen.
  4. Vorrichtung nach einem der Ansprüche 1 bis 3, wobei: die Magnetbewegungsstruktur darin einen Schlitz ausgebildet hat, der Magnet an der Magnetbewegungsstruktur an einer Seite des Schlitzes montiert ist und die Magnetbewegungsstruktur so konstruiert und angeordnet ist, dass der Reaktionsbehälter, der durch die Behälterträgereinheit gehalten wird, innerhalb des Schlitzes angeordnet ist, wenn die Magnetbewegungsstruktur in der zweiten Position ist, und dass der Reaktionsbehälter nicht in dem Schlitz angeordnet ist, wenn die Magnetbewegungsstruktur in der ersten Position ist, was erlaubt, dass der Reaktionsbehälter in die oder aus der Behälterträgereinheit ohne Störung durch die Magnetbewegungsstruktur bewegt wird, wenn die Magnetbewegungsstruktur in der ersten Position ist.
  5. Vorrichtung nach einem der Ansprüche 1 bis 4, wobei der Flüssigkeitstransfermechanismus eine oder mehrere Flüssigkeitsabgabedüsen, die konstruiert und angeordnet sind, um Flüssigkeit in einen oder mehrere Behälter abzugeben, wobei Gefäße eines Reaktionsbehälters durch die Behälterträgereinheit gehalten werden, und ein oder mehrere Absaugerröhrchen, die konstruiert und angeordnet sind, um Flüssigkeit aus einem oder mehreren Behältergefäßen eines Reaktionsbehälters, gehalten von der Behälterträgereinheit, abzusaugen, umfasst.
  6. Vorrichtung nach Anspruch 5, außerdem umfassend eine vertikal verschiebbare Röhrchenhaltevorrichtung, die konstruiert und angeordnet ist, um die Absaugerröhrchen zu tragen, um so eine Abwärtsbewegung der Absaugerröhrchen in den durch die Behälterträgereinheit gehaltenen Reaktionsbehälter zu erlauben, um die Flüssigkeitsinhalte aus dem Reaktionsbehälter abzusaugen, und um eine Aufwärtsbewegung der Absaugerröhrchen aus dem Reaktionsbehälter zu erlauben.
  7. Vorrichtung nach Anspruch 6, wobei: die Behälterträgereinheit konstruiert und angeordnet ist, um einen Reaktionsbehälter selektiv zu positionieren, der dadurch in einer Position bezüglich der Absaugerröhrchen und der Röhrchenhaltevorrichtung gehalten wird, um so zu erlauben, dass die Röhrchenhaltevorrichtung die Absaugerröhrchen in funktionellen Eingriff mit Kontaminationsbegrenzungselementen, die an dem Reaktionsbehälter angeordnet sind, absenkt, und wobei jedes der Absaugerröhrchen konstruiert und angeordnet ist, um ein Kontaminationsbegrenzungselement an einem Ende davon lösbar zu halten, um einen Kontakt zwischen den Flüssigkeitsinhalten, die durch das Absaugerröhrchen abgesaugt werden und einem äußeren Teil des Absaugerröhrchens zu beschränken.
  8. Vorrichtung nach einem der Ansprüche 1 bis 7, wobei die Behälterträgereinheit einen darin ausgebildeten Schlitz, der angepasst ist, um einen Reaktionsbehälter aufzunehmen, und Rückhalteelemente, die bezüglich des Schlitzes so angeordnet sind, dass sie Teile des Reaktionsbehälters greifen und den Reaktionsbehälter innerhalb des Schlitzes während eines magnetischen Trennungs-Reinigungs-Verfahrens zurückhalten, aufweist.
  9. Vorrichtung nach einem der Ansprüche 1 bis 8, wobei der Trägerbewegungsmechanismus konstruiert und angeordnet ist, um die Behälterträgereinheit in einer cyclischen Bewegung, die eine im allgemeinen horizontale Umlaufbahn umfasst, zu bewegen.
  10. Verfahren zum Durchführen eines magnetischen Trennungs-Reinigungs-Verfahren an einer Lösung, die magnetisch reagierende Partikel enthält und in einem Reaktionsbehälter enthalten ist, wobei das Verfahren umfasst: Bewegen wenigstens eines Magnets aus einer ersten Position zu einer zweiten Position bezüglich des Reaktionsbehälters, wobei der Magnet die magnetisch reagierenden Partikel zu einer inneren Oberfläche des Reaktionsbehälters anzieht, wenn der Magnet in der zweiten Position ist, und wobei die Anziehung der magnetisch reagierenden Partikel zu dem Magneten größer ist, wenn der Magnet in der zweiten Position ist, als wenn der Magnet in der ersten Position ist; Absaugen der Probenlösung aus dem Reaktionsbehälter, während der Magnet in der zweiten Position ist; Abgeben einer Waschlösung in den Reaktionsbehälter und Bewegen der Waschlösung durch Übertragen einer zyklischen Bewegung auf den Reaktionsbehälter, während der Magnet in der ersten Position ist, wobei der Reaktionsbehälter in einer im Wesentlichen fixierten Stellung durch das magnetische Trennungs-Reinigungs-Verfahren, das die Schritte der Bewegung, des Absaugens, des Abgebens und des Bewegens umfasst, gehalten wird.
  11. Verfahren nach Anspruch 10, wobei der Schritt des Haltens lösbares Halten eines Reaktionsbehälters in einer Behälterträgereinheit, die im Wesentlichen im Raum fixiert ist, umfasst.
  12. Verfahren nach Anspruch 10 oder 11, wobei der Schritt des Absaugens mit einem Flüssigkeitstransfermechanismus durchgeführt wird, der ein oder mehrere Absaugerröhrchen umfasst, die konstruiert und angeordnet sind, um selektiv in den Reaktionsbehälter bewegt zu werden, um die Probenlösung daraus abzusaugen.
  13. Verfahren nach Anspruch 12, wobei der Schritt des Absaugens außerdem Platzieren eines Kontaminationsbeschränkungselements auf ein distales Ende jedes der Absaugerröhrchen vor Bewegen der Absaugerröhrchen in den Reaktionsbehälter umfasst.
  14. Verfahren nach einem der Ansprüche 10 bis 13, wobei ein Bewegen wenigstens eines Magneten Montieren des Magneten an einer Magnetbewegungsstruktur und Bewegen des Magneten zwischen der ersten und zweiten Position durch Drehen der Magnetbewegungsstruktur um einen Drehpunkt umfasst.
  15. Verfahren nach einem der Ansprüche 10 bis 14, wobei der Schritt des Bewegens Übertragen einer zyklischen Bewegung mit im allgemeinen horizontaler Umlaufbahn auf den Reaktionsbehälter umfasst.
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