DE2204765A1 - Water-soluble disinfectant for destroying coccidial - oocysts and ascaride egss - comprising separately packed dithiocarbam - Google Patents

Abstract

A dry mixt. is prepd. from (a) a dry mixt. contg. a water-soluble dithiocarbamic acid or its salt and an emulsifier, and (b) a separately packaged solid, water-soluble, opt. org. acid. Component (a) or (b) can also contain a phenol and/or phenolate, and/or an additional solid, water-soluble bactericidal and fungicidal material. When mixed with H2O the combined components liberate CS2 in aqs. soln. thus avoiding the use of inflammable CS2. The mixt. is esp. useful for the control coccidiosis in poultry.

Description

Use of a water-soluble disinfectant mixture for complete Killing of eoccidial oocysts The well-known disinfectants, such as phenols or Formaldehyde, are specific against oocysts, the infectious forms of excretion Bimeric species, which causes coccidosis, an animal disease particularly in poultry, not effective enough on its own. The resistant protective coverings of the oocysts are hardly attacked by the known disinfectants, so that the destruction takes a very long time and is usually not complete. Am suitable means for Control of the oocysts was found in carbon disulfide. The carbon disulfide combined with phenols has a very good disinfecting effect (literature: R. Jungmann, Fh. Vet. Med. 20, 629 (1965)).

The disadvantages of using the carbon disulfide are also well-known, namely the sulphurous substance is because of its good lipoid solubility very poisonus. In addition, it has a low boiling point and forms with air explosive gas mixture.

Because of these properties, carbon disulfide is difficult to handle and in the application there is great danger.

The flammability can be achieved through niches with non-flammable solvents reduce, but not completely suppress.

The invention is based on the object of eliminating these disadvantages. The object is achieved by using a water-soluble disinfectant mixture for the complete destruction of coccidial oocysts and ascarid eggs, characterized in that, that one dry mixes of two separately packaged components a) a dry mix, the one soluble in water dithiocarbamic acid or its salt and an emulsifier and b) a solid, readily soluble in water, optionally containing organic acid.

There are known compounds that deal with carbon disulfide too implement stable compounds (Houben Weyl: Methods of Organic Chemistry, Volume 9, page 824).

These compounds include the salts of dithiocarbamic acid of the general formula 1 in which: R1, R2: lower alkyl radicals having 1 to 2 carbon atoms, where R1 and R2 can be identical or different; R: Na, h, ammonium or where R1 and R2 have the same meaning as above.

The dithiocarbaminate salts of the general formula I are available as dry, crystalline salts stable, while they are already weakly basic and weak easily decompose in an acidic solution.

The alkaline decomposition leads to thioureas with evolution of hydrogen sulfide. The compounds created in this way in the case of an alkaline solution are responsible for the disinfection effect unsatisfactory.

When acid is added, the compounds of the general formula I decompose according to the following reaction equation, releasing effective carbon disulfide: Reaction equation: The compounds of the general formula I dissolve very well in water. The carbon disulfide released during weak acidification remains dissolved in the aqueous solution and can be kept in the aqueous solution by means of suitable emulsifiers. This completely eliminates the high flammability and risk of explosion of the effective carbon disulfide. It is known, among other things (see above. Jungmann literature) that complete killing of the oocysts by simultaneous action of carbon disulfide with a phenol or

Phenol is achieved.

According to a preferred embodiment of the invention is therefore as a stable and oocyst-effective dry mixture of compounds of the general Formula I, a phenol salt and an emulsifier are used.

The disinfecting effect of this mixture is triggered by that you dissolve the stable and harmless, storable dry mixture in water and with a given acid, such as a solid, well water-soluble organic Acid, weakly acidic.

The effectiveness of the carbon disulfide released consists in its high lipoid solubility, which makes the oocyst's very resistant outer shell is attacked and thus a penetration of the strongly disinfecting phenol enables. It follows that complete effectiveness only through both components - is achieved.

Very suitable compounds for the above-described inventive compounds Mixture are the diethyl and dimethylaminodithicarbamic acid salts.

In addition to these dry mixes, other solid, readily water-soluble bactericidal and fungicidal agents added be making a broad, germicidal spectrum of activity is achieved.

The disinfectant mixture is used according to the invention in such a way that 1 kg of the separately packaged components are put together in 20 1 water dissolves and the solution on the surfaces or objects to be disinfected applies in a manner known per se.

Examples of the composition of the mixtures according to the invention in percent by weight: Example 1 a) 50% dimethylamino-dithiocarbamic acid sodium salt, 20% emulsifier (Atlas "BU 720") 10% p-chloro-m-cresol sodium salt b) 20% citric acid (Packed separately from a) Example 2 a) 50% diethylaminodithiocarbamic acid sodium salt 20% emulsifier (Warulat U, Bayer) 10% trichlorophenol sodium salt b) 20% oxalic acid (packed separately from a)) Example 3 a) 40% diethylaminoditihocarbamic acid diethylamine salt 20% emulsifier (Lutensit SC 9197, BASF) 15% p-chlorophenol Iiatric salt 5% cetylpyridinium chloride b) 20% tartaric acid (packed separately from a) Example 4 a) 50% diethylaminodithiocarbamic acid sodium salt 20% emulsifier (Lutensit SC 9197, BASF) b) 10% 2,4-dichlorophenol 20% citric acid (from a) separately packaged) Use of the disinfectant mixtures described in the examples and the results obtained: 800 g of the a) mixture described in the examples and 200 g of the b) acid described in the examples were stirred in 20 l of water. The disinfectant produced in this way was used against transported coccidial oocysts checked in the following way.

Broiler chicks of commercial production were used as experimental animals used in a large hatchery. These animals are caged for three weeks raised and received it a feed without coccidiostats. There is no possibility of infection with coccidial oocysts during this time. At the start of the experiment (day of infection) the broiler chicks are weight-wise divided into uniform groups of ten.

An infection material from sick chickens was isolated Eimeria tenella strain was used, which was passaged prior to each experiment.

On the transported oocysts of this strain, those in the examples Allowed the disinfectant to act as described above. In the control animals (infected control) 50,000 oocysts / individual animal were placed in the animal's crop Installed. The same number of oocysts was found in the test animals after exposure of the disinfectant is also installed in the animal's crop.

During the nine-day trial period, the general condition became of the animals observed. At the end of the experiment, all animals are killed, the changes in the mucous membrane observed in the appendix and determined the number of coccidial oocysts.

The test results are summarized in the following table.

Tabel Disinfectants Number of animals Exposure Pathological findings Oocyst detection after time of the appendix Example 1 10 30 min. OB 1 x + 9 x # Example 2 10 30 min. OB 10 x # Example 3 10 30 min. OB 10 x # IK 10 - very bloody 10 x ++++ swollen LK 10 - oB 10 x # IK = infected control, ie animals which were infected with untreated oocysts. LK = blank control, uninfected animals oB = no findings # = no oocysts detectable + = isolated oocysts detectable ++++ = massive amounts of oocysts detectable

Claims (3)

P a t e n t a n s p r ü c h e: 1. Application of a water-soluble Disinfectant mixture to completely kill coccidial oocysts and Ascarid eggs, characterized in that dry mixes are separated from two packaged components a) a dry mix, the one in water. soluble dithiocarbamic acid or their salt and an emulsifier and b) a solid, readily soluble in water, optionally contains organic acid. 2. Application according to claim 1, characterized in that the component a) or component b) contains a phenol and / or a phunolate. 3. Application according to claim 1 and 2, characterized in that the Component a) and / or component b) another, readily soluble in water, contains solid, bacteriUid and fungicidally active substance. Application and results of the disinfectant mixtures described in the examples 800 g of the a) mixture described in the examples and 200 g of that in the examples described b) acid are in 20 ltr. Stirring water. The disinfectant produced in this way For example, it is tested against coccidial oocysts in the following way. Broiler chicks of commercial production were used as experimental animals used in a large hatchery. These animals are caged for three weeks raised and receive a feed without coccidiostat. A possibility of infection with coccidial oocysts does not exist during this time. At the start of the experiment (day the infection) the broiler chicks are divided into groups of ten of uniform weight divided up. As an infectious material, one isolated from sick chickens is used Eimeria tenella strain used, which was passaged prior to each experiment. On the transported oocysts of this strain, those in the examples Allowed the disinfectant to act as described above. In the control animals (infected control) 50,000 oocysts / individual animal were in the goiter of the animal Installed. The test animals had the same number of oocysts after exposure of the disinfectant is also installed in the animal's crop. During the nine-day trial period, the general condition was of the animals observed. At the end of the experiment, all animals were killed, the changes in the mucous membrane observed in the appendix and determined the number of coccidial oocysts. The test results are summarized in a table.