DE3586858D1 - Transformation von aspergillus niger und dabei zu verwendende plasmide. - Google Patents

Transformation von aspergillus niger und dabei zu verwendende plasmide.

Info

Publication number
DE3586858D1
DE3586858D1 DE8585308827T DE3586858T DE3586858D1 DE 3586858 D1 DE3586858 D1 DE 3586858D1 DE 8585308827 T DE8585308827 T DE 8585308827T DE 3586858 T DE3586858 T DE 3586858T DE 3586858 D1 DE3586858 D1 DE 3586858D1
Authority
DE
Germany
Prior art keywords
niger
transformation
dna
aspergillus niger
plasmids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE8585308827T
Other languages
English (en)
Other versions
DE3586858T2 (de
Inventor
Francis Paul Buxton
David Ivor Gwynne
Roger Wayne Davies
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Koninklijke DSM NV
Original Assignee
Gist Brocades NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24723387&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE3586858(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Gist Brocades NV filed Critical Gist Brocades NV
Application granted granted Critical
Publication of DE3586858D1 publication Critical patent/DE3586858D1/de
Publication of DE3586858T2 publication Critical patent/DE3586858T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/8215Microorganisms
    • Y10S435/911Microorganisms using fungi
    • Y10S435/913Aspergillus
    • Y10S435/917Aspergillus niger
DE8585308827T 1984-12-05 1985-12-04 Transformation von aspergillus niger und dabei zu verwendende plasmide. Expired - Fee Related DE3586858T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/678,578 US4885249A (en) 1984-12-05 1984-12-05 Aspergillus niger transformation system

Publications (2)

Publication Number Publication Date
DE3586858D1 true DE3586858D1 (de) 1993-01-07
DE3586858T2 DE3586858T2 (de) 1993-05-19

Family

ID=24723387

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8585308827T Expired - Fee Related DE3586858T2 (de) 1984-12-05 1985-12-04 Transformation von aspergillus niger und dabei zu verwendende plasmide.

Country Status (8)

Country Link
US (1) US4885249A (de)
EP (1) EP0184438B1 (de)
JP (1) JPS61162168A (de)
AT (1) ATE82772T1 (de)
AU (1) AU590733B2 (de)
CA (1) CA1341342C (de)
DE (1) DE3586858T2 (de)
DK (1) DK175213B1 (de)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5723292A (en) * 1984-05-25 1998-03-03 Zymogenetics, Inc. Stable DNA constructs
US5364770A (en) * 1985-08-29 1994-11-15 Genencor International Inc. Heterologous polypeptides expressed in aspergillus
US6004785A (en) 1985-08-29 1999-12-21 Genencor International Inc. Heterologous polypeptides expressed in filamentous fungi, processes for making same, and vectors for making same
US5536661A (en) * 1987-03-10 1996-07-16 Novo Nordisk A/S Process for the production of protein products in aspergillus
US5766912A (en) * 1986-03-17 1998-06-16 Novo Nordisk A/S Humicola lipase produced in aspergillus
FI872102A (fi) * 1986-06-06 1987-12-07 Panlabs Inc Expressionssystem hos filamentala fungi.
GB8702475D0 (en) 1987-02-04 1987-03-11 Ciba Geigy Ag Expression system
US5447862A (en) * 1987-02-04 1995-09-05 Ciba-Geigy Corporation Pectin lyase genes of aspergillus niger
DE3854249T2 (de) * 1987-08-28 1996-02-29 Novo Nordisk As Rekombinante Humicola-Lipase und Verfahren zur Herstellung von rekombinanten Humicola-Lipasen.
JP2748347B2 (ja) * 1989-03-09 1998-05-06 キッコーマン 株式会社 アスペルギルス・ソーヤ形質転換体
ZA909987B (en) * 1990-01-29 1991-09-25 Ciba Geigy Novel fungal expression system
CA2093422C (en) * 1990-10-05 2001-04-03 DETERGENT COMPOSITIONS CONTAINING LOW CBH I CONTENT CELLULASE COMPOSITIONS
US5290474A (en) * 1990-10-05 1994-03-01 Genencor International, Inc. Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp
EP0570096B1 (de) * 1992-03-09 2001-11-14 Oji Paper Co., Ltd. Ornithin-Carbamoyl-Transferase Gen und seine Verwendung als Gen-Marker in einem Expression-System für die Herstellung von Proteinen bei Basidiomyceten
DK36592D0 (da) * 1992-03-20 1992-03-20 Novo Nordisk As Fremgangsmaade til fremstilling af proteiner
US5846802A (en) * 1992-04-15 1998-12-08 Buxton; Frank Fungal Protease
DK47493D0 (da) * 1993-04-27 1993-04-27 Novo Nordisk As Fremgangsmaade til fremstilling af ost
US5674728A (en) * 1993-11-03 1997-10-07 Novartis Corporation Aspergillus niger vacuolar aspartyl protease
US5643745A (en) * 1994-02-03 1997-07-01 University Of Hawaii Heterologous dimeric proteins produced in heterokaryons
US5683899A (en) * 1994-02-03 1997-11-04 University Of Hawaii Methods and compositions for combinatorial-based discovery of new multimeric molecules
US6268140B1 (en) 1994-02-03 2001-07-31 Neugenesis Combinatorial metabolic libraries
EP0770139B1 (de) * 1994-06-17 2003-12-10 Novozymes A/S EIN PILZ, IN DEM DAS areA-GEN VERÄNDERT IST, UND EIN areA-GEN AUS ASPERGILLUS ORYZAE
DE69532122T2 (de) * 1994-06-29 2004-07-08 Dsm Ip Assets B.V. Erhöhte produktion von beta-galaktosidase in aspergillus oryzae
US5776730A (en) * 1995-12-15 1998-07-07 University Of Hawaii Neurospora hosts for the production of recombinant proteins, and methods for producing same
CN1160465C (zh) * 1995-12-15 2004-08-04 诺沃奇梅兹有限公司 其中areA、pepC和/或pepE基因已被灭活的真菌
US7883872B2 (en) * 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US5811381A (en) * 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
GB9907461D0 (en) 1999-03-31 1999-05-26 King S College London Neurite regeneration
KR100618495B1 (ko) * 1998-10-06 2006-08-31 마크 아론 에말파브 섬유상의 진균성 숙주 영역에서의 형질전환 시스템:크리소스포륨속에서
US20050079573A1 (en) 1998-12-23 2005-04-14 Danisco A/S Proteins
US7157254B1 (en) * 1999-08-13 2007-01-02 Suntory Limited Microorganism secreting out lipid and process for producing the lipid and lipid balls having the lipid encapsulating therein by using the mircroorganism
GB0020498D0 (en) 2000-08-18 2000-10-11 Sterix Ltd Compound
AU3456102A (en) 2000-12-07 2002-06-18 Dsm Nv Protein hydrolysates enriched in peptides having a carboxy terminal proline residue
US8119171B2 (en) 2000-12-07 2012-02-21 Dsm Ip Assets B.V. Method for the prevention or reduction of haze in beverages
CZ20032454A3 (en) 2001-03-22 2004-03-17 Novo Nordisk Health Care Ag Coagulation factor vii derivative
GB0116453D0 (en) 2001-07-05 2001-08-29 Imp College Innovations Ltd Method
JP4088097B2 (ja) * 2002-04-26 2008-05-21 サントリー株式会社 高度不飽和脂肪酸含有脂質の製造方法
ES2525873T3 (es) 2002-06-07 2014-12-30 Dsm Ip Assets B.V. Método mejorado para la prevención o reducción del enturbiamiento en bebidas
AU2003258395A1 (en) * 2002-08-30 2004-03-19 Puratos Naamloze Vennootschap Myrothecium sp. transformation and expression system
JP2007508802A (ja) 2003-06-25 2007-04-12 ノボザイムス アクティーゼルスカブ 澱粉の加水分解法
EP1648996B1 (de) 2003-06-25 2012-03-14 Novozymes A/S Enzyme zur stärkeverarbeitung
ATE547519T1 (de) 2003-09-09 2012-03-15 Novo Nordisk Healthcare Ag Gerinnungsfaktor-vii-polypeptide
BR0318563B1 (pt) * 2003-10-31 2014-03-04 Pirelli Pneumatici S.P.A. Câmara expansível, método para fabricar a mesma, aparelho de vulcanização para pneus compreendendo a câmara expansível, e, processo para fabricar pneus de rodas de veículo
US20050181509A1 (en) * 2004-02-12 2005-08-18 The Penn State Research Foundation Dual selection based, targeted gene disruption method for fungi and fungus-like organisms
GB0403864D0 (en) 2004-02-20 2004-03-24 Ucl Ventures Modulator
ZA200702695B (en) 2004-10-15 2009-12-30 Neugenesis Corp Methods and compositions for combinatorial-based production of multivalent recombinant antigens
ES2552635T3 (es) 2004-12-22 2015-12-01 Novozymes A/S Polipéptidos con actividad de glucoamilasa y codificación de polinucleótidos
CN1298837C (zh) * 2005-07-28 2007-02-07 秘鸣 一种黑曲霉真菌及其在普洱茶生产中的应用
WO2007031559A2 (en) 2005-09-14 2007-03-22 Novo Nordisk Health Care Ag Human coagulation factor vii polypeptides
US7855279B2 (en) 2005-09-27 2010-12-21 Amunix Operating, Inc. Unstructured recombinant polymers and uses thereof
US7858360B2 (en) 2005-10-17 2010-12-28 Novozymes A/S Use of fungal mutants for expression of antibodies
EP3563688A1 (de) 2005-11-28 2019-11-06 DSM IP Assets B.V. Enzympräparate zur abgabe eines reinen geschmacks
GB0600406D0 (en) 2006-01-10 2006-02-15 Univ Bath Crystal
US9862956B2 (en) 2006-12-10 2018-01-09 Danisco Us Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
EP2102366A4 (de) 2006-12-10 2010-01-27 Dyadic International Inc Expression und hochdurchsatz-screening komplexer exprimierter dna-bibliotheken bei fadenpilzen
BRPI0807132A2 (pt) 2007-01-30 2018-12-04 Syngenta Participations Ag enzimas para o tratamento de lignocelulósicos, ácidos nucléicos que codificam as mesmas, e metodos e uso das mesmas
ES2492469T3 (es) 2007-02-20 2014-09-09 Dsm Ip Assets B.V. Nueva sialidasa
JP2010521188A (ja) 2007-03-22 2010-06-24 ディーエスエム アイピー アセッツ ビー.ブイ. 新規リシルオキシダーゼ
US8551751B2 (en) * 2007-09-07 2013-10-08 Dyadic International, Inc. BX11 enzymes having xylosidase activity
EP2201036A1 (de) 2007-08-08 2010-06-30 Novozymes Biopharma DK A/S Transferrinvarianten und konjugate
CN102348715B (zh) 2009-02-03 2017-12-08 阿穆尼克斯运营公司 延伸重组多肽和包含该延伸重组多肽的组合物
CN102369213A (zh) 2009-02-06 2012-03-07 诺维信生物制药丹麦公司 纯化方法
JP5936112B2 (ja) 2009-02-11 2016-06-15 アルブミディクス アクティーゼルスカブ アルブミン変異体及び複合体
AU2010272483B2 (en) 2009-07-17 2016-07-21 Omeros Corporation MASP isoforms as inhibitors of complement activation
AU2010290077C1 (en) 2009-08-24 2015-12-03 Bioverativ Therapeutics Inc. Coagulation factor IX compositions and methods of making and using same
JP5931738B2 (ja) 2009-12-01 2016-06-08 ノヴォ ノルディスク アー/エス 新規のペプチジルアルファ−ヒドロキシグリシンアルファ−アミド化リアーゼ
WO2011101339A2 (en) 2010-02-18 2011-08-25 Dsm Ip Assets B.V. Fermentation broth and filtration filter cake and uses thereof
CA2791841C (en) 2010-03-05 2023-01-03 Rigshospitalet Chimeric inhibitor molecules of complement activation
WO2012007446A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Use of a mutarotase in the production of dried powders
WO2012007445A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Mutarotase in crystallization
CA2811789A1 (en) 2010-10-06 2012-04-12 Bp Corporation North America Inc. Variant cbh i polypeptides with reduced product inhibition
CN103945861B (zh) 2011-09-12 2018-06-05 阿穆尼克斯运营公司 胰高血糖素样肽-2组合物及其制备和使用方法
PL2812024T3 (pl) 2012-02-09 2018-09-28 Var2 Pharmaceuticals Aps Celowanie glikanów siarczanu chondroityny
CA2864126A1 (en) 2012-02-15 2013-08-22 Biogen Idec Ma Inc. Recombinant factor viii proteins
WO2013122617A1 (en) 2012-02-15 2013-08-22 Amunix Operating Inc. Factor viii compositions and methods of making and using same
US20150307865A1 (en) 2012-10-15 2015-10-29 Novo Nordisk Health Care Ag Coagulation factor vii polypeptides
US10548953B2 (en) 2013-08-14 2020-02-04 Bioverativ Therapeutics Inc. Factor VIII-XTEN fusions and uses thereof
CN108472337B (zh) 2015-08-03 2022-11-25 比奥贝拉蒂治疗公司 因子ix融合蛋白以及其制备和使用方法
US20210278406A1 (en) 2018-07-13 2021-09-09 Varct Diagnostic Aps Isolation of circulating cells of fetal origin using recombinant malaria protein var2csa
EP4023757A1 (de) 2020-12-29 2022-07-06 The Protein Brewery B.V. Verfahren zur erzeugung von selektionsmarkerfreien transformanten von heterokaryotischen organismen
CN114958899A (zh) * 2022-05-30 2022-08-30 福州大学 一种能在黑曲霉细胞内自主复制的复制子

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4486533A (en) * 1982-09-02 1984-12-04 St. Louis University Filamentous fungi functional replicating extrachromosomal element
EP0191221A1 (de) * 1984-10-24 1986-08-20 The Regents Of The University Of California Vektoren zur Transformation von Ascomyceten
US4735901A (en) * 1985-01-14 1988-04-05 E. R. Squibb & Sons, Inc. Transformation of Candida albicans
EP0215594B2 (de) * 1985-08-29 2003-10-15 Genencor International, Inc. Expression von heterologen Polypeptiden in filamentösen Pilzen, Verfahren zu deren Herstellung und Vektoren zu deren Herstellung
FI864473A (fi) * 1985-11-20 1987-05-21 Panlabs Inc Sammansatta yttryckskassetter foer fungaltransformering.
US4935349A (en) * 1986-01-17 1990-06-19 Zymogenetics, Inc. Expression of higher eucaryotic genes in aspergillus

Also Published As

Publication number Publication date
DE3586858T2 (de) 1993-05-19
EP0184438B1 (de) 1992-11-25
ATE82772T1 (de) 1992-12-15
DK562685D0 (da) 1985-12-04
CA1341342C (en) 2002-02-26
DK175213B1 (da) 2004-07-12
JPS61162168A (ja) 1986-07-22
US4885249A (en) 1989-12-05
EP0184438A3 (en) 1988-01-13
AU5078085A (en) 1986-06-12
EP0184438A2 (de) 1986-06-11
DK562685A (da) 1986-06-06
AU590733B2 (en) 1989-11-16

Similar Documents

Publication Publication Date Title
DE3586858D1 (de) Transformation von aspergillus niger und dabei zu verwendende plasmide.
Bragg et al. Purification of a factor for both aerobic-driven and ATP-driven energy-dependent transhydrogenases of Escherichia coli
EP0249676A3 (de) Verfahren zur Genexpression in Pflanzen
ATE54167T1 (de) Promotoren fuer die expression von fremden genen in hefe, plasmide, die diese promotoren enthalten, sowie deren verwendung zur herstellung von polypeptiden.
IL66217A (en) Preparation and use of plasmids with genes for the biosynthesis of l-proline
ZA914714B (en) A cell having a certain function in a process,containing recombinant dna encoding at least one enzyme,recombinant dna,enzyme for which the recombinant dna encodes and uses thereof
MY102102A (en) Novel peptides
AU682140B2 (en) Multidrug resistance gene
BG40319A3 (en) Method for preparing recombinant desoxynucleinic acid containing genes of high organism and its cloning in microorganisms
DK453782A (da) Dna-sekvenser, rekombinant plasmid samt anvendelser deraf
ES2006383A6 (es) Procedimiento para la preparacion de vectores hibridos de levadura comprendiendo promotores de levadura represibles con los lugares de actuacion aguas arriba del gen ph05 de levadura.
IE840258L (en) Expression of gene products.
DE3381566D1 (de) Hepatitis-b-virus-gen enthaltendes rekombinantes plasmid, mit diesem rekombinanten plasmid transformierte hefe und herstellung von hepatitis-b-virus-oberflaechenantigen.
GB1530651A (en) Glass
DE2963374D1 (en) Recombinant dna, method for preparing it and production of foreign proteins by unicellular hosts containing it
JPS5658488A (en) Refined "sake" with low alcohol content
EP0183654A3 (de) Drehelektrode aus Kohle oder Graphit für Elektroöfen mit offenem oder verdecktem Lichtbogen
GB9003010D0 (en) Gene expression in yeast cells
WO1992011370A3 (de) Von proteinen des hepatitis c-virus abgeleitete polypeptide und deren verwendung
GR3033685T3 (en) Mutant interleukin 6 with improved biological activity over wild interleukin 6.
ES8606402A1 (es) Un procedimiento para preparar una molecula de adn recom- biante
EP0174585A3 (de) Promotoren und deren Anwendung zur Expression von nichtfusierten prokaryontischen oder enkaryontischen Proteinen in Hefe
ATE64153T1 (de) Plasmide zur erhoehten produktion von penicillin g-amidase.
EP0165614A3 (de) Hybridplasmid und dasselbe enthaltender Mikroorganismus
EP0155594A3 (de) Klonierte Plasmide von Corynebacterium

Legal Events

Date Code Title Description
8363 Opposition against the patent
8327 Change in the person/name/address of the patent owner

Owner name: DSM N.V., HEERLEN, NL

8339 Ceased/non-payment of the annual fee