DE3751042T2 - T7-DNS-Polymerase. - Google Patents

T7-DNS-Polymerase.

Info

Publication number
DE3751042T2
DE3751042T2 DE3751042T DE3751042T DE3751042T2 DE 3751042 T2 DE3751042 T2 DE 3751042T2 DE 3751042 T DE3751042 T DE 3751042T DE 3751042 T DE3751042 T DE 3751042T DE 3751042 T2 DE3751042 T2 DE 3751042T2
Authority
DE
Germany
Prior art keywords
dna
incubating
molecule
nucleotide base
dna molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE3751042T
Other languages
English (en)
Other versions
DE3751042D1 (de
Inventor
Stanley Tabor
Charles C Richardson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard College
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26671499&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE3751042(T2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US07/003,227 external-priority patent/US4795699A/en
Application filed by Harvard College filed Critical Harvard College
Publication of DE3751042D1 publication Critical patent/DE3751042D1/de
Application granted granted Critical
Publication of DE3751042T2 publication Critical patent/DE3751042T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli
DE3751042T 1987-01-14 1987-12-24 T7-DNS-Polymerase. Expired - Lifetime DE3751042T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/003,227 US4795699A (en) 1987-01-14 1987-01-14 T7 DNA polymerase
US07/132,569 US4942130A (en) 1987-01-14 1987-12-14 T7 DNA polymerase

Publications (2)

Publication Number Publication Date
DE3751042D1 DE3751042D1 (de) 1995-03-16
DE3751042T2 true DE3751042T2 (de) 1995-05-24

Family

ID=26671499

Family Applications (9)

Application Number Title Priority Date Filing Date
DE3750288T Expired - Lifetime DE3750288T2 (de) 1987-01-14 1987-12-24 T7-DNS-Polymerase.
DE199292202037T Pending DE516245T1 (de) 1987-01-14 1987-12-24 Dns-sequenzierung.
DE9090201138T Expired - Fee Related DE3781809T2 (de) 1987-01-14 1987-12-24 T7-dns-polymerase.
DE198787311435T Pending DE265293T1 (de) 1987-01-14 1987-12-24 T7-dns-polymerase.
DE199090201139T Pending DE386858T1 (de) 1987-01-14 1987-12-24 T7-dns-polymerase.
DE3751042T Expired - Lifetime DE3751042T2 (de) 1987-01-14 1987-12-24 T7-DNS-Polymerase.
DE199090201140T Pending DE386859T1 (de) 1987-01-14 1987-12-24 T7-dns-polymerase.
DE3789623T Expired - Lifetime DE3789623T4 (de) 1987-01-14 1987-12-24 T7-DNS-Polymerase.
DE3789623A Expired - Fee Related DE3789623D1 (de) 1987-01-14 1987-12-24 T7-DNS-Polymerase.

Family Applications Before (5)

Application Number Title Priority Date Filing Date
DE3750288T Expired - Lifetime DE3750288T2 (de) 1987-01-14 1987-12-24 T7-DNS-Polymerase.
DE199292202037T Pending DE516245T1 (de) 1987-01-14 1987-12-24 Dns-sequenzierung.
DE9090201138T Expired - Fee Related DE3781809T2 (de) 1987-01-14 1987-12-24 T7-dns-polymerase.
DE198787311435T Pending DE265293T1 (de) 1987-01-14 1987-12-24 T7-dns-polymerase.
DE199090201139T Pending DE386858T1 (de) 1987-01-14 1987-12-24 T7-dns-polymerase.

Family Applications After (3)

Application Number Title Priority Date Filing Date
DE199090201140T Pending DE386859T1 (de) 1987-01-14 1987-12-24 T7-dns-polymerase.
DE3789623T Expired - Lifetime DE3789623T4 (de) 1987-01-14 1987-12-24 T7-DNS-Polymerase.
DE3789623A Expired - Fee Related DE3789623D1 (de) 1987-01-14 1987-12-24 T7-DNS-Polymerase.

Country Status (19)

Country Link
US (1) US4942130A (de)
EP (5) EP0386857B1 (de)
JP (7) JP2584644B2 (de)
KR (1) KR930005660B1 (de)
CN (1) CN1031251A (de)
AT (2) ATE118039T1 (de)
AU (1) AU604824B2 (de)
CA (2) CA1340628C (de)
DD (1) DD301731A9 (de)
DE (9) DE3750288T2 (de)
DK (1) DK13288A (de)
ES (5) ES2063243T3 (de)
FI (1) FI880131A (de)
GR (2) GR880300103T1 (de)
HU (1) HUT46069A (de)
IL (1) IL85073A (de)
NO (1) NO880126L (de)
NZ (1) NZ223039A (de)
RO (1) RO107769B1 (de)

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US4962020A (en) * 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
GB8827167D0 (en) * 1988-11-21 1988-12-29 Apothekernes Lab In vitro mutagenesis
US5001050A (en) * 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
US5047342A (en) * 1989-08-10 1991-09-10 Life Technologies, Inc. Cloning and expression of T5 DNA polymerase
US5541099A (en) * 1989-08-10 1996-07-30 Life Technologies, Inc. Cloning and expression of T5 DNA polymerase reduced in 3'-to-5' exonuclease activity
US5270179A (en) * 1989-08-10 1993-12-14 Life Technologies, Inc. Cloning and expression of T5 DNA polymerase reduced in 3'- to-5' exonuclease activity
GB8926269D0 (en) * 1989-11-21 1990-01-10 Dynal As Plasmid
CA2036965A1 (en) * 1990-02-26 1991-08-27 Robert J. Duronio Method for protein n-myristoylation
US5322785A (en) 1990-04-26 1994-06-21 New England Biolabs, Inc. Purified thermostable DNA polymerase obtainable from thermococcus litoralis
WO1993005174A1 (en) * 1991-08-30 1993-03-18 Syngenix Limited Nucleic acid polymerase amplification
US7465539B1 (en) 1993-02-19 2008-12-16 Takara Bio, Inc. DNA polymerases with enhanced length of primer extension
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US6410277B1 (en) 1993-02-19 2002-06-25 Takara Shuzo Co., Ltd. DNA polymersases with enhanced length of primer extension
US5547859A (en) * 1993-08-02 1996-08-20 Goodman; Myron F. Chain-terminating nucleotides for DNA sequencing methods
US7312052B1 (en) 1993-12-08 2007-12-25 Stratagene California Polymerase compositions and uses thereof
US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
EP0655506B1 (de) * 1994-10-17 1996-09-18 President And Fellows Of Harvard College DNS Polymerase mit veränderter Nukleotid-Bindungstelle
US5948614A (en) * 1995-09-08 1999-09-07 Life Technologies, Inc. Cloned DNA polymerases from thermotoga maritima and mutants thereof
US5789168A (en) * 1996-05-01 1998-08-04 Visible Genetics Inc. Method for amplification and sequencing of nucleic acid polymers
US5830657A (en) * 1996-05-01 1998-11-03 Visible Genetics Inc. Method for single-tube sequencing of nucleic acid polymers
US5888736A (en) * 1995-12-22 1999-03-30 Visible Genetics, Inc. Method, compositions and kit for detection and identification of microorganisms
US6413718B1 (en) 1996-05-01 2002-07-02 Visible Genetics Inc. Method for sequencing of nucleic acid polymers
US6214555B1 (en) 1996-05-01 2001-04-10 Visible Genetics Inc. Method compositions and kit for detection
US6083699A (en) * 1996-05-01 2000-07-04 Visible Genetics Inc. Method for bi-directional sequencing of nucleic acid polymers
US5827716A (en) * 1996-07-30 1998-10-27 Amersham Life Science, Inc. Modified Pol-II type DNA polymerases
US6291164B1 (en) 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
US5876934A (en) * 1996-12-18 1999-03-02 Pharmacia Biotech Inc. DNA sequencing method
US6306588B1 (en) 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
JP4245084B2 (ja) 1997-03-21 2009-03-25 ストラタジーン ポリメラーゼ増強因子（ｐｅｆ）抽出物、ｐｅｆタンパク質複合体、単離されたｐｅｆタンパク質、並びに精製及び単離方法
EP1149175A2 (de) * 1999-01-11 2001-10-31 President And Fellows Of Harvard College Isothermische dns-amplifizierung
CN1313394A (zh) * 2000-03-10 2001-09-19 上海博德基因开发有限公司 一种新的多肽——dna聚合酶10和编码这种多肽的多核苷酸
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7222059B2 (en) 2001-11-15 2007-05-22 Siemens Medical Solutions Diagnostics Electrophoretic trace simulator
EP1697534B1 (de) 2003-12-01 2010-06-02 Life Technologies Corporation Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
EP2297343A1 (de) * 2008-05-16 2011-03-23 New England Biolabs, Inc. Enzymreagentien zur amplifikation von polynukleotiden in gegenwart von inhibitoren
KR101417989B1 (ko) * 2013-02-08 2014-07-09 주식회사 엔지노믹스 이중가닥 dna 말단의 단일가닥 길이 조절 방법
US10793841B2 (en) 2017-06-30 2020-10-06 Codexis, Inc. T7 RNA polymerase variants
JP2020530267A (ja) 2017-06-30 2020-10-22 コデクシス， インコーポレイテッド Ｔ７ ｒｎａポリメラーゼバリアント
CN114774453B (zh) * 2022-03-14 2023-04-21 湖北大学 一种运动发酵单胞菌基因表达严谨调控系统的构建方法和应用

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Also Published As

Publication number Publication date
ES2056366T3 (es) 1994-10-01
FI880131A0 (fi) 1988-01-13
DE516245T1 (de) 1993-04-29
NO880126L (no) 1988-07-15
EP0265293A2 (de) 1988-04-27
ES2063243T3 (es) 1995-01-01
JPS63237798A (ja) 1988-10-04
EP0265293B1 (de) 1994-07-27
ATE118039T1 (de) 1995-02-15
ES2068323T3 (es) 1995-04-16
DE3789623T2 (de) 1994-07-21
KR880009126A (ko) 1988-09-14
HUT46069A (en) 1988-09-28
JPH0670772A (ja) 1994-03-15
RO107769B1 (ro) 1993-12-30
DE3781809D1 (en) 1992-10-22
EP0386859B1 (de) 1995-02-01
GR3006442T3 (de) 1993-06-21
DE3750288T2 (de) 1994-11-10
JPH0746990A (ja) 1995-02-21
ATE109207T1 (de) 1994-08-15
NZ223039A (en) 1990-07-26
EP0386857B1 (de) 1992-09-16
FI880131A (fi) 1988-07-15
EP0516245A1 (de) 1992-12-02
DK13288A (da) 1988-07-15
DE3750288D1 (de) 1994-09-01
DE3781809T2 (de) 1993-03-18
CA1341512C (en) 2006-09-12
JPH06141898A (ja) 1994-05-24
CN1031251A (zh) 1989-02-22
DE3751042D1 (de) 1995-03-16
EP0386858A2 (de) 1990-09-12
AU1022488A (en) 1988-07-21
JPH0670773A (ja) 1994-03-15
JPH05308998A (ja) 1993-11-22
GR880300103T1 (en) 1988-10-21
DE3789623T4 (de) 1994-12-01
KR930005660B1 (ko) 1993-06-24
IL85073A0 (en) 1988-06-30
EP0265293A3 (en) 1989-12-13
EP0386859A3 (de) 1991-07-03
DE3789623D1 (de) 1994-05-19
JP2584644B2 (ja) 1997-02-26
ES2060569T1 (es) 1994-12-01
EP0386858B1 (de) 1994-04-13
EP0386859A2 (de) 1990-09-12
US4942130A (en) 1990-07-17
JPH06237765A (ja) 1994-08-30
JP2606777B2 (ja) 1997-05-07
NO880126D0 (no) 1988-01-13
DE265293T1 (de) 1988-08-11
CA1340628C (en) 1999-07-06
ES2001837T3 (es) 1994-10-16
EP0386857A3 (de) 1991-07-10
DK13288D0 (da) 1988-01-13
IL85073A (en) 1994-10-07
AU604824B2 (en) 1991-01-03
EP0386857A2 (de) 1990-09-12
EP0386858A3 (de) 1991-07-03
DE386859T1 (de) 1991-07-04
DD301731A9 (de) 1993-08-26
DE386858T1 (de) 1991-07-04
ES2001837A4 (es) 1988-07-01

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