DE3889918D1 - Nachweis einzelner moleküle. - Google Patents

Nachweis einzelner moleküle.

Info

Publication number
DE3889918D1
DE3889918D1 DE3889918T DE3889918T DE3889918D1 DE 3889918 D1 DE3889918 D1 DE 3889918D1 DE 3889918 T DE3889918 T DE 3889918T DE 3889918 T DE3889918 T DE 3889918T DE 3889918 D1 DE3889918 D1 DE 3889918D1
Authority
DE
Germany
Prior art keywords
single molecules
detecting single
detecting
molecules
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE3889918T
Other languages
English (en)
Other versions
DE3889918T2 (de
Inventor
Edgar Shera
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Department of Energy
Original Assignee
US Department of Energy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by US Department of Energy filed Critical US Department of Energy
Publication of DE3889918D1 publication Critical patent/DE3889918D1/de
Application granted granted Critical
Publication of DE3889918T2 publication Critical patent/DE3889918T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6402—Atomic fluorescence; Laser induced fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N2015/0038—Investigating nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N15/0205—Investigating particle size or size distribution by optical means, e.g. by light scattering, diffraction, holography or imaging
    • G01N15/0211—Investigating a scatter or diffraction pattern
    • G01N2015/0222—Investigating a scatter or diffraction pattern from dynamic light scattering, e.g. photon correlation spectroscopy
    • G01N2015/1027—
DE3889918T 1987-10-07 1988-09-16 Nachweis einzelner moleküle. Expired - Fee Related DE3889918T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/105,376 US4793705A (en) 1987-10-07 1987-10-07 Single molecule tracking
PCT/US1988/003193 WO1989003525A1 (en) 1987-10-07 1988-09-16 Single molecule tracking

Publications (2)

Publication Number Publication Date
DE3889918D1 true DE3889918D1 (de) 1994-07-07
DE3889918T2 DE3889918T2 (de) 1995-01-19

Family

ID=22305471

Family Applications (1)

Application Number Title Priority Date Filing Date
DE3889918T Expired - Fee Related DE3889918T2 (de) 1987-10-07 1988-09-16 Nachweis einzelner moleküle.

Country Status (6)

Country Link
US (1) US4793705A (de)
EP (1) EP0381694B1 (de)
JP (1) JPH03500449A (de)
CA (1) CA1326556C (de)
DE (1) DE3889918T2 (de)
WO (1) WO1989003525A1 (de)

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US6403311B1 (en) 1997-02-12 2002-06-11 Us Genomics Methods of analyzing polymers using ordered label strategies
DE69825601T2 (de) 1997-02-12 2005-04-28 Chan, Eugene Y, Brookline Verfahren zur analyse von polymeren
US6049380A (en) * 1997-11-12 2000-04-11 Regents Of The University Of California Single molecule identification using selected fluorescence characteristics
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SE9800360D0 (sv) * 1998-02-06 1998-02-06 Goeteborg University Science I Method, apparatus and flow cell for high sensitivity detection of fluorescent molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
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US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
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US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
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US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
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WO2004104178A2 (en) 2003-05-15 2004-12-02 Xy, Inc. Efficient haploid cell sorting for flow cytometer systems
US20090088982A1 (en) * 2003-07-31 2009-04-02 Fukushima Noelle H Co-detection of single polypeptide and polynucleotide molecules
US20080021674A1 (en) * 2003-09-30 2008-01-24 Robert Puskas Methods for Enhancing the Analysis of Particle Detection
US7169560B2 (en) * 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
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US7572640B2 (en) * 2004-09-28 2009-08-11 Singulex, Inc. Method for highly sensitive detection of single protein molecules labeled with fluorescent moieties
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US9040305B2 (en) * 2004-09-28 2015-05-26 Singulex, Inc. Method of analysis for determining a specific protein in blood samples using fluorescence spectrometry
US8685711B2 (en) * 2004-09-28 2014-04-01 Singulex, Inc. Methods and compositions for highly sensitive detection of molecules
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
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US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
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US8821799B2 (en) 2007-01-26 2014-09-02 Palo Alto Research Center Incorporated Method and system implementing spatially modulated excitation or emission for particle characterization with enhanced sensitivity
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JP6396911B2 (ja) 2012-10-15 2018-09-26 ナノセレクト バイオメディカル， インコーポレイテッド 粒子を選別するためのシステム、装置、および、方法
CN103091294B (zh) * 2013-01-21 2015-08-05 中南民族大学 应用于骨髓干细胞运动轨迹追踪的细胞标记检测方法
CN107561053B (zh) * 2016-07-01 2020-04-28 清华大学 一种单分子检测方法
CN108195801B (zh) * 2017-11-17 2020-11-24 北京林业大学 单分子水平观测气孔保卫细胞膜蛋白分布和动态的方法

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JPS61186854A (ja) * 1985-02-14 1986-08-20 Fuji Photo Film Co Ltd 超純水中のバクテリア数測定装置

Also Published As

Publication number Publication date
EP0381694A1 (de) 1990-08-16
EP0381694B1 (de) 1994-06-01
JPH03500449A (ja) 1991-01-31
WO1989003525A1 (en) 1989-04-20
US4793705A (en) 1988-12-27
CA1326556C (en) 1994-01-25
EP0381694A4 (en) 1991-08-28
DE3889918T2 (de) 1995-01-19

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Legal Events

Date Code Title Description
8380 Miscellaneous part iii

Free format text: DER INHABER IST ZU AENDERN IN: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, OAKLAND, CALIF., US

8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee