DE59509938D1 - Verfahren zur Quantifizierung von Nukleinsaüren - Google Patents

Verfahren zur Quantifizierung von Nukleinsaüren

Info

Publication number
DE59509938D1
DE59509938D1 DE59509938T DE59509938T DE59509938D1 DE 59509938 D1 DE59509938 D1 DE 59509938D1 DE 59509938 T DE59509938 T DE 59509938T DE 59509938 T DE59509938 T DE 59509938T DE 59509938 D1 DE59509938 D1 DE 59509938D1
Authority
DE
Germany
Prior art keywords
target
quantifying nucleic
contg
methods
nucleic acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE59509938T
Other languages
English (en)
Inventor
Dr Falkner
Dr Haemmerle
Dr Himmelspach
Dr Kohl
Prof Dr Dorner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baxter Vaccine AG
Original Assignee
Baxter Vaccine AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AT183194A external-priority patent/AT401062B/de
Priority claimed from AT224594A external-priority patent/ATA224594A/de
Application filed by Baxter Vaccine AG filed Critical Baxter Vaccine AG
Application granted granted Critical
Publication of DE59509938D1 publication Critical patent/DE59509938D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701Specific hybridization probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
DE59509938T 1994-09-26 1995-09-26 Verfahren zur Quantifizierung von Nukleinsaüren Expired - Fee Related DE59509938D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT183194A AT401062B (de) 1994-09-26 1994-09-26 Verfahren zur quantifizierung von nukleinsäuren
AT224594A ATA224594A (de) 1994-12-02 1994-12-02 Verfahren zur quantifizierung von nukleinsäuren

Publications (1)

Publication Number Publication Date
DE59509938D1 true DE59509938D1 (de) 2002-01-24

Family

ID=25596921

Family Applications (1)

Application Number Title Priority Date Filing Date
DE59509938T Expired - Fee Related DE59509938D1 (de) 1994-09-26 1995-09-26 Verfahren zur Quantifizierung von Nukleinsaüren

Country Status (8)

Country Link
US (1) US5789153A (de)
EP (1) EP0714988B1 (de)
JP (1) JPH08107798A (de)
AT (1) ATE210735T1 (de)
CA (1) CA2159044A1 (de)
DE (1) DE59509938D1 (de)
DK (1) DK0714988T3 (de)
ES (1) ES2169753T3 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5814442A (en) * 1994-06-10 1998-09-29 Georgetown University Internally controlled virion nucleic acid amplification reaction for quantitation of virion and virion nucleic acid
AT406019B (de) * 1995-05-08 2000-01-25 Immuno Ag Verfahren zur herstellung eines arzneimittels enthaltend ein oder mehrere plasmaderivate
GB9519638D0 (en) * 1995-09-26 1995-11-29 Dynal As Method
US6265152B1 (en) * 1995-12-22 2001-07-24 Visible Genetics Inc. Method and kit for evaluation of HIV mutations
AT406778B (de) * 1996-04-09 2000-09-25 Immuno Ag Verfahren zur desintegration von nucleinsäuren und herstellung von qualitätsgesicherten biologischen produkten
AT406373B (de) * 1997-02-27 2000-04-25 Immuno Ag Verfahren zur reinigung von faktor viii/vwf-komplex mittels kationenaustauscherchromatographie
AT405403B (de) 1997-02-27 1999-08-25 Immuno Ag Reinigung von von willebrand-faktor durch kationenaustauscherchromatographie
DE19752898A1 (de) * 1997-11-28 1999-08-05 Centeon Pharma Gmbh Verfahren zum Nachweis hoher Vierenkonzentrationen im Blutplasma und/oder Blutserum mittels der Polymerasekettenreaktion
AT407160B (de) * 1998-06-04 2001-01-25 Immuno Ag Verfahren zur bestimmung von antigenen
US6455692B1 (en) 1998-08-04 2002-09-24 Transgenomic, Inc. Method of concentrating polynucleotides using MIPC
GB9902422D0 (en) * 1999-02-03 1999-03-24 Lgc Teddington Limited Reference material for nucleic acid amplification
AT410218B (de) 1999-08-20 2003-03-25 Baxter Ag Verfahren zur herstellung eines qualitätsgesicherten pools biologischer proben
US6379889B1 (en) * 1999-11-04 2002-04-30 Agilent Technologies, Inc. Multiplexing methods for identifying nucleic acids using denaturing liquid chromatography
AT409382B (de) 1999-12-22 2002-07-25 Baxter Ag Verfahren zur detektion, unterscheidung und quantifizierung von freien und eingekapselten nukleinsäuren in einer probe
US7790368B1 (en) 2000-09-22 2010-09-07 Hitachi, Ltd. Method for analyzing nucleic acid
US6312929B1 (en) * 2000-12-22 2001-11-06 Cepheid Compositions and methods enabling a totally internally controlled amplification reaction
US6982029B2 (en) * 2001-05-07 2006-01-03 Spectramedix Llc Electrophoretic method and system having internal lane standards for color calibration
US20030207310A1 (en) * 2002-05-01 2003-11-06 Zhaowei Liu System and method for determining the sizes and quantity of polynucleotides with capillary array electrophoresis
ATE496141T1 (de) * 2003-12-06 2011-02-15 Abbott Lab Verfahren und system zum analysieren von reaktionen unter verwendung eines informationssystems
CA2573532C (en) 2004-07-13 2014-01-07 Gen-Probe Incorporated Compositions and methods for detection of hepatitis a virus nucleic acid
US7794659B2 (en) 2005-03-10 2010-09-14 Gen-Probe Incorporated Signal measuring system having a movable signal measuring device
JP5564795B2 (ja) * 2009-01-06 2014-08-06 株式会社島津製作所 Dna定量方法、及び遺伝子解析方法
EP2598655B1 (de) * 2010-07-29 2016-10-05 F.Hoffmann-La Roche Ag Qualitativer und quantitativer nachweis mikrobieller nukleinsäuren
US8877464B2 (en) 2010-07-29 2014-11-04 Roche Molecular Systems, Inc. Qualitative and quantitative detection of microbial nucleic acids
JP6121591B2 (ja) * 2010-07-29 2017-04-26 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 微生物核酸の定性的および定量的検出
AU2011315926B2 (en) 2010-10-14 2015-12-10 Rheonix, Inc. Quantitative multiplexed identification of nucleic acid targets
WO2012095748A2 (en) * 2011-01-14 2012-07-19 Smiths Detection - Watford Limited Single tube quantitative polymerase chain reaction (pcr)
US9932628B2 (en) 2012-07-27 2018-04-03 Gen-Probe Incorporated Dual reference calibration method and system for quantifying polynucleotides
DE102018213027A1 (de) * 2018-08-03 2020-02-06 Robert Bosch Gmbh Reaktionsgemisch, Verfahren und Kit zur Durchführung einer quantitativen Echtzeit-PCR

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
GB2272443B (en) * 1991-06-10 1995-10-25 Lucky Ltd Nucleotide and amino acid sequences of Korean hepatitis C virus
JP2739004B2 (ja) * 1992-01-16 1998-04-08 三菱電機株式会社 半導体装置
AU4248193A (en) * 1992-05-15 1993-12-13 New England Deaconess Hospital Quantitation of viral rna by competitive polymerase chain reaction
WO1994010342A1 (en) * 1992-10-30 1994-05-11 The Rogosin Institute Method for quantifying nucleic acids
WO1994020640A1 (en) * 1993-03-05 1994-09-15 Genelabs Technologies, Inc. Method for hiv quantitation
ES2131699T3 (es) * 1993-07-09 1999-08-01 Akzo Nobel Nv Procedimiento mejorado de cuantificacion del acido nucleico.
US5523204A (en) * 1993-12-10 1996-06-04 Becton Dickinson And Company Detection of nucleic acids in cells by strand displacement amplification
US5491063A (en) * 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes

Also Published As

Publication number Publication date
ATE210735T1 (de) 2001-12-15
EP0714988A3 (de) 1997-04-16
US5789153A (en) 1998-08-04
CA2159044A1 (en) 1996-03-27
EP0714988B1 (de) 2001-12-12
ES2169753T3 (es) 2002-07-16
JPH08107798A (ja) 1996-04-30
DK0714988T3 (da) 2002-04-08
EP0714988A2 (de) 1996-06-05

Similar Documents

Publication Publication Date Title
DE59509938D1 (de) Verfahren zur Quantifizierung von Nukleinsaüren
DK0497784T3 (da) Kvantificering af nukleinsyrer under anvendelse af polymerasekædereaktionen
FI104329B1 (fi) Menetelmä kohdenukleiinihapon määrittämiseksi ja menetelmässä käytettävä koepakkaus
ATE230801T1 (de) Verfahren zur detektion von zielnukleinsäure, verfahren zu ihrer quantifizierung und pyrylium- verbindungen zur chemilumineszenz-analyse
DE59509937D1 (de) Verfahren zur Quantifizierung von genomischer DNA
ATE557103T1 (de) Verfahren zur erkennung von nukleinsäuresequenzen in urin
DE69316372D1 (de) Eliminierung von falschen negativen in nuklein-säuren
ATE253645T1 (de) Verfahren zur bestimmung von säugetier nukleinsäuren aus stuhlproben und dafür benötigte reagenzien
DE69533660D1 (de) Homogenes Verfahren zum Nachweis von Doppelstrang-Nukleinsäuren mittels fluoreszierender Farbstoffe und dafür nützliche Kits
ATE206764T1 (de) Verfahren zum sensitiven nachweis von nukleinsäuren
ATE243759T1 (de) Verfahren zur dämpfung der fluoreszene in lösung von fluoreszenzmarkierten oligonucleotidsonden
FI900602A0 (fi) Bestaemning.
ATE141651T1 (de) Verfahren zum nachweis und zur identifizierung von nukleinsäure enthaltenden teilen
FI831688L (fi) Test foer esterasaktivitet i ett flytande specimen
GB2355073A (en) Quantitative analysis of gene expression using pcr
EP0128018A3 (en) Molecular genetic probe, and method of forming same, assay technique and kit using said molecular genetic probe
FI902934A0 (fi) Foerfarande foer bestaemning av nukleinsyra.
ATE290607T1 (de) Floureszenzintensitäts-test für eine protein- oder peptidbindung an nukleinsäuren
SE8801011D0 (sv) Time-resolved electroluminescence in solution
Holzapfel-Pschorn et al. Sensitive methods for the determination of microbial activities in water samples using fluorigenic substrates
DE69941690D1 (de) Methoden, testsätze und kompositionen zur detektion und quantifizierung von zielsequenzen
ATE427991T1 (de) Verfahren zur in vivo quantifizierung von nukleinsauren
ATE333519T1 (de) Electrochemisches verfahren zum nachweis von nukleinsäuren
EP0912765B1 (de) Bestimmung von analyten unter verwendung zweier markierungen
ATE269417T1 (de) Verfahren zur erkennung und messung von gespleisten nukleinsäuren

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee