DE60015368D1 - Verfahren zur befestigung von oligonukleotiden auf ein substrat - Google Patents

Verfahren zur befestigung von oligonukleotiden auf ein substrat

Info

Publication number
DE60015368D1
DE60015368D1 DE60015368T DE60015368T DE60015368D1 DE 60015368 D1 DE60015368 D1 DE 60015368D1 DE 60015368 T DE60015368 T DE 60015368T DE 60015368 T DE60015368 T DE 60015368T DE 60015368 D1 DE60015368 D1 DE 60015368D1
Authority
DE
Germany
Prior art keywords
substrate
oligonucleotide primer
containing multiple
glass
glass substrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60015368T
Other languages
English (en)
Other versions
DE60015368T2 (de
Inventor
Xiaodong Zhao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Global Life Sciences Solutions USA LLC
Original Assignee
Amersham Biosciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Biosciences Corp filed Critical Amersham Biosciences Corp
Publication of DE60015368D1 publication Critical patent/DE60015368D1/de
Application granted granted Critical
Publication of DE60015368T2 publication Critical patent/DE60015368T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
DE60015368T 1999-09-02 2000-08-24 Verfahren zur befestigung von oligonukleotiden auf ein substrat Expired - Fee Related DE60015368T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/388,702 US6528319B1 (en) 1999-09-02 1999-09-02 Method for anchoring oligonucleotides to a substrate
US388702 1999-09-02
PCT/US2000/023255 WO2001016152A2 (en) 1999-09-02 2000-08-24 Method for anchoring oligonucleotides to a substrate

Publications (2)

Publication Number Publication Date
DE60015368D1 true DE60015368D1 (de) 2004-12-02
DE60015368T2 DE60015368T2 (de) 2006-02-02

Family

ID=23535172

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60015368T Expired - Fee Related DE60015368T2 (de) 1999-09-02 2000-08-24 Verfahren zur befestigung von oligonukleotiden auf ein substrat

Country Status (7)

Country Link
US (1) US6528319B1 (de)
EP (1) EP1210356B1 (de)
AT (1) ATE280773T1 (de)
AU (1) AU6800400A (de)
CA (1) CA2381761A1 (de)
DE (1) DE60015368T2 (de)
WO (1) WO2001016152A2 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020076832A1 (en) * 2000-06-02 2002-06-20 Pirrung Michael C. Method of attaching a biopolymer to a solid support
WO2003029494A1 (en) * 2001-10-03 2003-04-10 Micrologix Biotech Inc. Attachment of thiophosphate tethered oligonucleotides to a solid surface
AU2002363537A1 (en) * 2001-11-07 2003-05-19 Integrigen, Inc. Method for generation of modular polynucleotides using solid supports
DE10159904A1 (de) * 2001-12-06 2003-07-03 Adnagen Ag Oligonukleotidanordnung, Verfahren zum Nukleotidnachweis sowie Vorrichtung hierfür
US20050074781A1 (en) * 2003-10-02 2005-04-07 Herbert von Schroeder Nucleic acid braided J-probes
GB0326073D0 (en) * 2003-11-07 2003-12-10 Solexa Ltd Improvements in or relating to polynucleotide arrays
US7781187B2 (en) 2005-12-30 2010-08-24 Corning Incorporated Fluorescent dyes
JP5560039B2 (ja) 2006-10-05 2014-07-23 マサチューセッツ インスティテュート オブ テクノロジー ハイスループット分析のための多機能のコード化された粒子
US9476101B2 (en) 2010-06-07 2016-10-25 Firefly Bioworks, Inc. Scanning multifunctional particles

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5118605A (en) * 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US5700637A (en) 1988-05-03 1997-12-23 Isis Innovation Limited Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays
US5237016A (en) * 1989-01-05 1993-08-17 Siska Diagnostics, Inc. End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
US5888819A (en) 1991-03-05 1999-03-30 Molecular Tool, Inc. Method for determining nucleotide identity through primer extension
SE501439C2 (sv) 1993-06-22 1995-02-13 Pharmacia Lkb Biotech Sätt och anordning för analys av polynukleotidsekvenser
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5770365A (en) 1995-08-25 1998-06-23 Tm Technologies, Inc. Nucleic acid capture moieties
US5770151A (en) 1996-06-05 1998-06-23 Molecular Dynamics, Inc. High-speed liquid deposition device for biological molecule array formation
US5837860A (en) 1997-03-05 1998-11-17 Molecular Tool, Inc. Covalent attachment of nucleic acid molecules onto solid-phases via disulfide bonds

Also Published As

Publication number Publication date
US6528319B1 (en) 2003-03-04
WO2001016152A3 (en) 2001-07-26
DE60015368T2 (de) 2006-02-02
CA2381761A1 (en) 2001-03-08
ATE280773T1 (de) 2004-11-15
AU6800400A (en) 2001-03-26
EP1210356A2 (de) 2002-06-05
WO2001016152A2 (en) 2001-03-08
EP1210356B1 (de) 2004-10-27

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: GE HEALTHCARE BIO-SCIENCES CORP., PISCATAWAY, N.J.

8339 Ceased/non-payment of the annual fee