DE60023936D1 - Methoden zur verwendung von randomisierten zinkfingerprotein-bibliotheken zur identifizierung von genfunktionen - Google Patents

Methoden zur verwendung von randomisierten zinkfingerprotein-bibliotheken zur identifizierung von genfunktionen

Info

Publication number
DE60023936D1
DE60023936D1 DE60023936T DE60023936T DE60023936D1 DE 60023936 D1 DE60023936 D1 DE 60023936D1 DE 60023936 T DE60023936 T DE 60023936T DE 60023936 T DE60023936 T DE 60023936T DE 60023936 D1 DE60023936 D1 DE 60023936D1
Authority
DE
Germany
Prior art keywords
methods
randomized
general functions
protein libraries
identifying general
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60023936T
Other languages
English (en)
Other versions
DE60023936T2 (de
Inventor
Christopher Case
Qiang Liu
J Rebar
Alan P Wolffe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sangamo Therapeutics Inc
Original Assignee
Sangamo Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sangamo Biosciences Inc filed Critical Sangamo Biosciences Inc
Publication of DE60023936D1 publication Critical patent/DE60023936D1/de
Application granted granted Critical
Publication of DE60023936T2 publication Critical patent/DE60023936T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
DE60023936T 1999-12-06 2000-12-06 Methoden zur verwendung von randomisierten zinkfingerprotein-bibliotheken zur identifizierung von genfunktionen Expired - Lifetime DE60023936T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US45610099A 1999-12-06 1999-12-06
US456100 1999-12-06
PCT/US2000/033086 WO2001040798A2 (en) 1999-12-06 2000-12-06 Methods of using randomized libraries of zinc finger proteins for the identification of gene function

Publications (2)

Publication Number Publication Date
DE60023936D1 true DE60023936D1 (de) 2005-12-15
DE60023936T2 DE60023936T2 (de) 2006-05-24

Family

ID=23811426

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60023936T Expired - Lifetime DE60023936T2 (de) 1999-12-06 2000-12-06 Methoden zur verwendung von randomisierten zinkfingerprotein-bibliotheken zur identifizierung von genfunktionen

Country Status (9)

Country Link
US (4) US6503717B2 (de)
EP (1) EP1236045B1 (de)
AT (1) ATE309536T1 (de)
AU (1) AU776576B2 (de)
CA (1) CA2394850C (de)
DE (1) DE60023936T2 (de)
HK (1) HK1049515B (de)
IL (3) IL150069A0 (de)
WO (1) WO2001040798A2 (de)

Families Citing this family (267)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7013219B2 (en) * 1999-01-12 2006-03-14 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US20030104526A1 (en) 1999-03-24 2003-06-05 Qiang Liu Position dependent recognition of GNN nucleotide triplets by zinc fingers
US7030215B2 (en) * 1999-03-24 2006-04-18 Sangamo Biosciences, Inc. Position dependent recognition of GNN nucleotide triplets by zinc fingers
US20020164575A1 (en) * 1999-09-14 2002-11-07 Sangamo Biosciences, Inc., A Delaware Corporation Gene identification
DE60023936T2 (de) * 1999-12-06 2006-05-24 Sangamo Biosciences Inc., Richmond Methoden zur verwendung von randomisierten zinkfingerprotein-bibliotheken zur identifizierung von genfunktionen
US20020061512A1 (en) * 2000-02-18 2002-05-23 Kim Jin-Soo Zinc finger domains and methods of identifying same
US20020055112A1 (en) * 2000-08-26 2002-05-09 Nila Patil Methods for reducing complexity of nucleic acid samples
US20020164634A1 (en) * 2000-08-26 2002-11-07 Perlegen Sciences, Inc. Methods for reducing complexity of nucleic acid samples
US20040110177A1 (en) * 2001-02-02 2004-06-10 Axel Ullrich Method for identifying functional nucleic acids
WO2003016571A1 (en) * 2001-08-17 2003-02-27 Toolgen, Inc. Zinc finger domain libraries
EP1451297A4 (de) * 2001-12-07 2006-06-28 Toolgen Inc Phänotyp-screening chimärer proteine
US20040259258A1 (en) * 2001-12-07 2004-12-23 Kim Jin-Soo Regulation of prokaryotic gene expression with zinc finger proteins
US8106255B2 (en) 2002-01-23 2012-01-31 Dana Carroll Targeted chromosomal mutagenasis using zinc finger nucleases
EP1481087A4 (de) * 2002-02-07 2005-12-21 Scripps Research Inst Zinkfingerbibliotheken
US20030180777A1 (en) * 2002-03-12 2003-09-25 Victor Bartsevich Rapid identification of transcriptional regulatory domains
CA2497913C (en) 2002-09-05 2014-06-03 California Institute Of Technology Use of chimeric nucleases to stimulate gene targeting
WO2004022575A2 (en) * 2002-09-05 2004-03-18 Toolgen, Inc. Bioinformatics analysis of cellular effects of artificial transcription factors
US20050069896A1 (en) * 2002-09-12 2005-03-31 Horvitz H. Robert Rb pathway and chromatin remodeling genes that antagonize let-60 Ras signaling
US20070178454A1 (en) * 2002-10-21 2007-08-02 Joung J K Context sensitive paralell optimization of zinc finger dna binding domains
WO2004099367A2 (en) * 2002-10-23 2004-11-18 The General Hospital Corporation Methods for producing zinc finger proteins that bind to extended dna target sequences
EP1579005A4 (de) * 2002-11-15 2007-07-25 Sangamo Biosciences Inc Verfahren und zusammensetzungen zur analyse regulatorischer sequenzen
JP2006508675A (ja) * 2002-12-09 2006-03-16 トゥールゲン・インコーポレイテッド 調節性ジンクフィンガータンパク質
US20090124514A1 (en) * 2003-02-26 2009-05-14 Perlegen Sciences, Inc. Selection probe amplification
US20060183132A1 (en) * 2005-02-14 2006-08-17 Perlegen Sciences, Inc. Selection probe amplification
US20050100911A1 (en) * 2003-08-06 2005-05-12 Perlegen Sciences, Inc. Methods for enriching populations of nucleic acid samples
US11311574B2 (en) 2003-08-08 2022-04-26 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
US8409861B2 (en) 2003-08-08 2013-04-02 Sangamo Biosciences, Inc. Targeted deletion of cellular DNA sequences
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US20120196370A1 (en) 2010-12-03 2012-08-02 Fyodor Urnov Methods and compositions for targeted genomic deletion
US7972854B2 (en) 2004-02-05 2011-07-05 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US20080131962A1 (en) * 2006-05-25 2008-06-05 Sangamo Biosciences, Inc. Engineered cleavage half-domains
AU2005287278B2 (en) 2004-09-16 2011-08-04 Sangamo Biosciences, Inc. Compositions and methods for protein production
CA2613517A1 (en) 2005-06-27 2007-01-04 Exelixis, Inc. Pyrazole based lxr modulators
US7718131B2 (en) * 2005-07-06 2010-05-18 Genetix Limited Methods and apparatus for imaging and processing of samples in biological sample containers
US20070148674A1 (en) * 2005-12-23 2007-06-28 Wisconsin Alumni Research Foundation (Warf) Optical fingerprinting of nucleic acid sequences
WO2007106603A2 (en) * 2006-01-06 2007-09-20 The Scripps Research Institute Specific labeling of proteins with zinc finger tags and use of zinc-finger-tagged proteins for analysis
KR100756055B1 (ko) * 2006-01-27 2007-09-07 연세대학교 산학협력단 신생혈관 생성을 조절하는 재조합 아데노바이러스
WO2007106488A2 (en) * 2006-03-13 2007-09-20 Wyeth Modulators of gluconeogenesis
JP2009537140A (ja) * 2006-05-19 2009-10-29 サンガモ バイオサイエンシーズ， インコーポレイテッド ジヒドロ葉酸還元酵素の不活性化のための方法及び組成物
WO2007139982A2 (en) 2006-05-25 2007-12-06 Sangamo Biosciences, Inc. Methods and compositions for gene inactivation
ES2532002T3 (es) 2006-08-11 2015-03-23 Dow Agrosciences Llc Recombinación homóloga mediada por nucleasas con dedos de zinc
AU2007319881B2 (en) 2006-11-13 2012-05-17 Sangamo Therapeutics, Inc. Zinc finger nuclease for targeting the human glucocorticoid receptor locus
CA2692453C (en) * 2007-07-12 2018-01-09 Trevor Collingwood Methods and compositions for inactivating alpha 1,6 fucosyltransferase (fut8) gene expression
US8563314B2 (en) 2007-09-27 2013-10-22 Sangamo Biosciences, Inc. Methods and compositions for modulating PD1
DK2205749T3 (en) * 2007-09-27 2016-08-22 Dow Agrosciences Llc MODIFIED PROTEINS zinc finger, which target the 5-enolpyruvylshikimate-3-phosphate synthase genes
JP2010539931A (ja) * 2007-09-27 2010-12-24 サンガモ バイオサイエンシーズ， インコーポレイテッド ジンクフィンガーヌクレアーゼを使用したゼブラフィッシュにおけるゲノム編集
WO2009054985A1 (en) 2007-10-25 2009-04-30 Sangamo Biosciences, Inc. Methods and compositions for targeted integration
WO2009146179A1 (en) * 2008-04-15 2009-12-03 University Of Iowa Research Foundation Zinc finger nuclease for the cftr gene and methods of use thereof
KR101802393B1 (ko) 2008-06-10 2017-11-28 상가모 테라퓨틱스, 인코포레이티드 Ｂａｘ- 및 ｂａｋ-결함 세포주들의 제조 방법 및 조성물
CA2734235C (en) 2008-08-22 2019-03-26 Sangamo Biosciences, Inc. Methods and compositions for targeted single-stranded cleavage and targeted integration
WO2010053518A2 (en) 2008-10-29 2010-05-14 Sangamo Biosciences, Inc. Methods and compositions for inactivating glutamine synthetase gene expression
AU2009322964B2 (en) 2008-12-04 2014-10-09 Sangamo Therapeutics, Inc. Genome editing in rats using zinc-finger nucleases
AU2010211057B2 (en) * 2009-02-04 2014-12-18 Sangamo Therapeutics, Inc. Methods and compositions for treating neuropathies
JP5932632B2 (ja) 2009-03-20 2016-06-15 サンガモ バイオサイエンシーズ， インコーポレイテッド 改変された亜鉛フィンガータンパク質を使用したｃｘｃｒ４の修飾
CA2756833C (en) * 2009-04-09 2019-11-19 Sangamo Biosciences, Inc. Targeted integration into stem cells
EP2461819A4 (de) 2009-07-28 2013-07-31 Sangamo Biosciences Inc Verfahren und zusammensetzungen zur behandlung erblicher trinukleotiderkrankungen
US8586526B2 (en) 2010-05-17 2013-11-19 Sangamo Biosciences, Inc. DNA-binding proteins and uses thereof
US8956828B2 (en) * 2009-11-10 2015-02-17 Sangamo Biosciences, Inc. Targeted disruption of T cell receptor genes using engineered zinc finger protein nucleases
CN102812034B (zh) * 2010-01-22 2016-08-03 陶氏益农公司 靶向基因组改造
ES2751916T3 (es) 2010-02-08 2020-04-02 Sangamo Therapeutics Inc Semidominios de escisión genomanipulados
JP2013518602A (ja) 2010-02-09 2013-05-23 サンガモ バイオサイエンシーズ， インコーポレイテッド 部分的に一本鎖のドナー分子による標的化ゲノム改変
JP5952263B2 (ja) 2010-04-26 2016-07-13 サンガモ バイオサイエンシーズ， インコーポレイテッド ジンクフィンガーヌクレアーゼを使ったｒｏｓａ遺伝子座のゲノム編集
CA2805442C (en) 2010-07-21 2020-05-12 Sangamo Biosciences, Inc. Methods and compositions for modification of an hla locus
EP2622090B1 (de) 2010-09-27 2019-06-19 Sangamo Therapeutics, Inc. Zusammensetzungen zur hemmung des eindringens von viren in zellen
US9175280B2 (en) 2010-10-12 2015-11-03 Sangamo Biosciences, Inc. Methods and compositions for treating hemophilia B
US9267123B2 (en) 2011-01-05 2016-02-23 Sangamo Biosciences, Inc. Methods and compositions for gene correction
WO2013012674A1 (en) 2011-07-15 2013-01-24 The General Hospital Corporation Methods of transcription activator like effector assembly
US10323236B2 (en) 2011-07-22 2019-06-18 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
US9161995B2 (en) 2011-07-25 2015-10-20 Sangamo Biosciences, Inc. Methods and compositions for alteration of a cystic fibrosis transmembrane conductance regulator (CFTR) gene
CA2848417C (en) 2011-09-21 2023-05-02 Sangamo Biosciences, Inc. Methods and compositions for regulation of transgene expression
US8895264B2 (en) 2011-10-27 2014-11-25 Sangamo Biosciences, Inc. Methods and compositions for modification of the HPRT locus
US9458205B2 (en) 2011-11-16 2016-10-04 Sangamo Biosciences, Inc. Modified DNA-binding proteins and uses thereof
WO2013130824A1 (en) 2012-02-29 2013-09-06 Sangamo Biosciences, Inc. Methods and compositions for treating huntington's disease
AU2013251558B2 (en) * 2012-04-25 2019-01-03 Regeneron Pharmaceuticals, Inc. Nuclease-mediated targeting with large targeting vectors
KR102091298B1 (ko) * 2012-05-02 2020-03-19 다우 아그로사이언시즈 엘엘씨 말산 탈수소효소의 표적화된 변형
EP2847338B1 (de) 2012-05-07 2018-09-19 Sangamo Therapeutics, Inc. Verfahren und zusammensetzungen zur nukleasevermittelten gezielten integration von transgenen
US9890364B2 (en) 2012-05-29 2018-02-13 The General Hospital Corporation TAL-Tet1 fusion proteins and methods of use thereof
JP6329537B2 (ja) 2012-07-11 2018-05-23 サンガモ セラピューティクス， インコーポレイテッド 生物学的薬剤の送達のための方法および組成物
US10648001B2 (en) 2012-07-11 2020-05-12 Sangamo Therapeutics, Inc. Method of treating mucopolysaccharidosis type I or II
ES2613691T3 (es) 2012-07-11 2017-05-25 Sangamo Biosciences, Inc. Métodos y composiciones para el tratamiento de enfermedades por almacenamiento lisosomal
EA039384B1 (ru) 2012-08-29 2022-01-20 Сангамо Байосаенсез, Инк. Белок "цинковые пальцы" для модулирования экспрессии гена bcl11a и способ модулирования экспрессии глобинового гена
EP3750999B1 (de) 2012-09-04 2022-06-29 The Scripps Research Institute Chimäre polypeptide mit gerichteter bindungsspezifität
UA118090C2 (uk) 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив
UA119135C2 (uk) 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб отримання трансгенної рослини
RU2665811C2 (ru) 2012-09-07 2018-09-04 ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи Локусы fad3 для выполнения операций и соответствующие связывающиеся со специфическими сайтами-мишенями белки, способные к вызову направленных разрывов
HUE050797T2 (hu) 2012-10-10 2021-01-28 Sangamo Therapeutics Inc T-sejt módosító vegyületek és alkalmazásaik
US20150267176A1 (en) 2012-10-12 2015-09-24 The General Hospital Corporation Transcription activator-like effector (tale) - lysine-specific demethylase 1 (lsd1) fusion proteins
US9255250B2 (en) 2012-12-05 2016-02-09 Sangamo Bioscience, Inc. Isolated mouse or human cell having an exogenous transgene in an endogenous albumin gene
WO2014089541A2 (en) 2012-12-07 2014-06-12 Haplomics, Inc. Factor viii mutation repair and tolerance induction
EP3345991B1 (de) 2013-02-07 2019-09-04 The General Hospital Corporation Tale-transkriptionsaktivatoren
AU2014218621B2 (en) 2013-02-25 2019-11-07 Sangamo Therapeutics, Inc. Methods and compositions for enhancing nuclease-mediated gene disruption
CN112301024A (zh) 2013-03-15 2021-02-02 通用医疗公司 使用RNA引导的FokI核酸酶(RFN)提高RNA引导的基因组编辑的特异性
US11332719B2 (en) * 2013-03-15 2022-05-17 The Broad Institute, Inc. Recombinant virus and preparations thereof
US10760064B2 (en) 2013-03-15 2020-09-01 The General Hospital Corporation RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci
CN105208866B (zh) 2013-03-21 2018-11-23 桑格摩生物治疗股份有限公司 使用工程化锌指蛋白核酸酶靶向断裂t细胞受体基因
LT3456831T (lt) 2013-04-16 2021-09-10 Regeneron Pharmaceuticals, Inc. Tikslinis žiurkės genomo modifikavimas
US10604771B2 (en) 2013-05-10 2020-03-31 Sangamo Therapeutics, Inc. Delivery methods and compositions for nuclease-mediated genome engineering
US9873894B2 (en) 2013-05-15 2018-01-23 Sangamo Therapeutics, Inc. Methods and compositions for treatment of a genetic condition
US10011850B2 (en) 2013-06-21 2018-07-03 The General Hospital Corporation Using RNA-guided FokI Nucleases (RFNs) to increase specificity for RNA-Guided Genome Editing
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
JP6588438B2 (ja) 2013-08-28 2019-10-09 サンガモ セラピューティクス， インコーポレイテッド Ｄｎａ結合ドメインと切断ドメインとを連結するための組成物
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
CN116836957A (zh) 2013-10-17 2023-10-03 桑格摩生物科学股份有限公司 用于核酸酶介导的基因组工程改造的递送方法和组合物
US10117899B2 (en) 2013-10-17 2018-11-06 Sangamo Therapeutics, Inc. Delivery methods and compositions for nuclease-mediated genome engineering in hematopoietic stem cells
JP6633532B2 (ja) 2013-11-04 2020-01-22 ダウ アグロサイエンシィズ エルエルシー 最適なトウモロコシ遺伝子座
NZ719494A (en) 2013-11-04 2017-09-29 Dow Agrosciences Llc Optimal maize loci
NZ746567A (en) 2013-11-04 2019-09-27 Dow Agrosciences Llc Optimal soybean loci
EP3363903B1 (de) 2013-11-07 2024-01-03 Editas Medicine, Inc. Crispr-assoziierte verfahren und zusammensetzungen mit regelnden grnas
AU2014346424B2 (en) 2013-11-11 2020-09-17 Sangamo Therapeutics, Inc. Methods and compositions for treating Huntington's Disease
EP3068881B1 (de) 2013-11-13 2019-02-27 Children's Medical Center Corporation Nukleasevermittelte regulierung der genexpression
ES2813367T3 (es) 2013-12-09 2021-03-23 Sangamo Therapeutics Inc Métodos y composiciones para ingeniería genómica
AU2014360811B2 (en) 2013-12-11 2017-05-18 Regeneron Pharmaceuticals, Inc. Methods and compositions for the targeted modification of a genome
US9068179B1 (en) 2013-12-12 2015-06-30 President And Fellows Of Harvard College Methods for correcting presenilin point mutations
RS60514B1 (sr) 2014-02-03 2020-08-31 Sangamo Therapeutics Inc Postupci i sastavi za tretman beta talasemije
JP6606088B2 (ja) 2014-02-24 2019-11-13 サンガモ セラピューティクス， インコーポレイテッド ヌクレアーゼ媒介性標的化組み込みのための方法および組成物
WO2015143046A2 (en) 2014-03-18 2015-09-24 Sangamo Biosciences, Inc. Methods and compositions for regulation of zinc finger protein expression
WO2015164748A1 (en) 2014-04-24 2015-10-29 Sangamo Biosciences, Inc. Engineered transcription activator like effector (tale) proteins
BR112016025849A2 (pt) 2014-05-08 2017-10-17 Chdi Foundation Inc métodos e composições para o tratamento da doença de huntington
CA2947622A1 (en) 2014-05-13 2015-11-19 Sangamo Biosciences, Inc. Genome editing methods and compositions for prevention or treatment of a disease
EP3152319A4 (de) 2014-06-05 2017-12-27 Sangamo BioSciences, Inc. Verfahren und zusammensetzungen zur nukleasekonstruktion
RS60359B1 (sr) 2014-06-06 2020-07-31 Regeneron Pharma Postupci i kompozicije za modifikovanje ciljanog lokusa
EP3158066B1 (de) 2014-06-23 2021-05-12 The General Hospital Corporation Genomweite unbeeinflusste, durch sequenzierung (guide-seq) beurteilte identifizierung von dsbs
RU2711740C2 (ru) 2014-06-26 2020-01-21 Регенерон Фармасьютикалз, Инк. Способы и композиции для нацеленных генетических модификаций и способы их применения
DK3169778T3 (da) 2014-07-14 2023-12-18 Univ Washington State Nanos-knockout, der eliminerer kimbaneceller
CN107075483A (zh) 2014-07-15 2017-08-18 朱诺治疗学股份有限公司 用于过继细胞治疗的工程改造的细胞
WO2016014837A1 (en) 2014-07-25 2016-01-28 Sangamo Biosciences, Inc. Gene editing for hiv gene therapy
US9816074B2 (en) 2014-07-25 2017-11-14 Sangamo Therapeutics, Inc. Methods and compositions for modulating nuclease-mediated genome engineering in hematopoietic stem cells
AU2015298571B2 (en) 2014-07-30 2020-09-03 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
US9616090B2 (en) 2014-07-30 2017-04-11 Sangamo Biosciences, Inc. Gene correction of SCID-related genes in hematopoietic stem and progenitor cells
PT3194570T (pt) 2014-09-16 2021-08-06 Sangamo Therapeutics Inc Métodos e composições para manipulação e correção de genoma mediada por nuclease em células estaminais hematopoiéticas
EP3845655A1 (de) 2014-10-01 2021-07-07 The General Hospital Corporation Verfahren zur erhöhung der effizienz von nucleaseinduzierter homologiegerichteter reparatur
RU2020134412A (ru) 2014-11-21 2020-11-11 Регенерон Фармасьютикалз, Инк. Способы и композиции для нацеленной генетической модификации с использованием парных гидовых рнк
US10889834B2 (en) 2014-12-15 2021-01-12 Sangamo Therapeutics, Inc. Methods and compositions for enhancing targeted transgene integration
CN107208113A (zh) 2014-12-19 2017-09-26 瑞泽恩制药公司 用于通过单步多重靶向进行靶向遗传修饰的方法和组合物
US20180002379A1 (en) 2015-01-21 2018-01-04 Sangamo Therapeutics, Inc. Methods and compositions for identification of highly specific nucleases
EP3277823B1 (de) 2015-04-03 2023-09-13 Dana-Farber Cancer Institute, Inc. Zusammensetzung und verfahren zur genomänderung von b-zellen
US10179918B2 (en) 2015-05-07 2019-01-15 Sangamo Therapeutics, Inc. Methods and compositions for increasing transgene activity
AU2016261927B2 (en) 2015-05-12 2022-04-07 Sangamo Therapeutics, Inc. Nuclease-mediated regulation of gene expression
EP3302525A2 (de) 2015-06-05 2018-04-11 Novartis AG Verfahren und zusammensetzungen zur diagnose, behandlung und überwachung der behandlung von shank3-mangelbedingten erkrankungen
US9957501B2 (en) 2015-06-18 2018-05-01 Sangamo Therapeutics, Inc. Nuclease-mediated regulation of gene expression
AU2016291778B2 (en) 2015-07-13 2021-05-06 Sangamo Therapeutics, Inc. Delivery methods and compositions for nuclease-mediated genome engineering
MA42895A (fr) 2015-07-15 2018-05-23 Juno Therapeutics Inc Cellules modifiées pour thérapie cellulaire adoptive
RU2018107842A (ru) 2015-08-06 2019-09-09 Дзе Кьюрейторз Оф Дзе Юниверсити Оф Миссури Устойчивые к патогенам животные, имеющие модифицированные гены cd163
US9926546B2 (en) 2015-08-28 2018-03-27 The General Hospital Corporation Engineered CRISPR-Cas9 nucleases
US9512446B1 (en) 2015-08-28 2016-12-06 The General Hospital Corporation Engineered CRISPR-Cas9 nucleases
CA3000816A1 (en) 2015-09-11 2017-03-16 The General Hospital Corporation Full interrogation of nuclease dsbs and sequencing (find-seq)
BR112018005779A2 (pt) 2015-09-23 2018-10-09 Sangamo Therapeutics, Inc. repressores de htt e usos dos mesmos
EP3356526B1 (de) 2015-09-30 2021-08-25 The General Hospital Corporation Umfassende in-vitro-meldung von spaltungsereignissen durch sequenzierung (circle-seq)
IL258821B (en) 2015-10-23 2022-07-01 Harvard College Nucleobase editors and their uses
JP6909212B2 (ja) 2015-10-28 2021-07-28 サンガモ セラピューティクス， インコーポレイテッド 肝臓特異的コンストラクト、第ｖｉｉｉ因子発現カセット、およびその使用の方法
AU2016361350B2 (en) 2015-11-23 2023-04-06 Sangamo Therapeutics, Inc. Methods and compositions for engineering immunity
KR20180088911A (ko) 2015-12-18 2018-08-07 상가모 테라퓨틱스, 인코포레이티드 Mhc 세포 수용체의 표적화된 붕괴
JP7128741B2 (ja) 2015-12-18 2022-08-31 サンガモ セラピューティクス， インコーポレイテッド Ｔ細胞受容体の標的化破壊
ES2895430T3 (es) 2016-01-15 2022-02-21 Univ Minnesota Métodos y composiciones para el tratamiento de enfermedad neurológica
US10724020B2 (en) 2016-02-02 2020-07-28 Sangamo Therapeutics, Inc. Compositions for linking DNA-binding domains and cleavage domains
ES2901215T3 (es) 2016-05-27 2022-03-21 Aadigen Llc Péptidos y nanopartículas para la liberación intracelular de moléculas de edición del genoma
ES2938210T3 (es) 2016-07-13 2023-04-05 Vertex Pharma Métodos, composiciones y kits para aumentar la eficiencia de edición del genoma
KR102547316B1 (ko) 2016-08-03 2023-06-23 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
CN109804066A (zh) 2016-08-09 2019-05-24 哈佛大学的校长及成员们 可编程cas9-重组酶融合蛋白及其用途
BR112019003100A2 (pt) 2016-08-24 2019-07-09 Sangamo Therapeutics Inc regulação da expressão gênica usando nucleases manipuladas
BR112019003327A2 (pt) 2016-08-24 2019-07-02 Sangamo Therapeutics Inc nucleases manipuladas de alvo específico
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
CA3035534A1 (en) 2016-09-07 2018-03-15 Sangamo Therapeutics, Inc. Modulation of liver genes
EP3523422A1 (de) 2016-10-05 2019-08-14 FUJIFILM Cellular Dynamics, Inc. Erzeugung reifer linien aus induzierten pluripotenten stammzellen mit mecp2-unterbrechung
CN110381963A (zh) 2016-10-13 2019-10-25 朱诺治疗学股份有限公司 涉及色氨酸代谢途径调节剂的免疫治疗方法和组合物
KR102622411B1 (ko) 2016-10-14 2024-01-10 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
EP3528852A4 (de) 2016-10-20 2020-06-03 Sangamo Therapeutics, Inc. Verfahren und zusammensetzungen zur behandlung von morbus fabry
AU2017347928A1 (en) 2016-10-31 2019-05-09 Sangamo Therapeutics, Inc. Gene correction of scid-related genes in hematopoietic stem and progenitor cells
AU2017367722B2 (en) 2016-12-01 2024-02-01 Sangamo Therapeutics, Inc. Tau modulators and methods and compositions for delivery thereof
CA3045323A1 (en) 2016-12-02 2018-06-07 Juno Therapeutics, Inc. Engineered b cells and related compositions and methods
EP4276187A3 (de) 2016-12-08 2024-01-17 Case Western Reserve University Verfahren und zusammensetzungen zur erhöhung der herstellung von funktionalem myelin
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
TW201839136A (zh) 2017-02-06 2018-11-01 瑞士商諾華公司 治療血色素異常症之組合物及方法
WO2018165504A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression of pain by gene editing
US11542496B2 (en) 2017-03-10 2023-01-03 President And Fellows Of Harvard College Cytosine to guanine base editor
GB2575930A (en) 2017-03-23 2020-01-29 Harvard College Nucleobase editors comprising nucleic acid programmable DNA binding proteins
CA3058189A1 (en) 2017-04-03 2018-10-11 Encoded Therapeutics, Inc. Tissue selective transgene expression
WO2018200943A1 (en) 2017-04-28 2018-11-01 Acuitas Therapeutics, Inc. Novel carbonyl lipids and lipid nanoparticle formulations for delivery of nucleic acids
JP2020518245A (ja) 2017-05-03 2020-06-25 サンガモ セラピューティクス， インコーポレイテッド 嚢胞性線維症膜貫通コンダクタンス制御因子（ｃｆｔｒ）遺伝子の改変のための方法および組成物
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
CN110959040A (zh) 2017-05-25 2020-04-03 通用医疗公司 具有改进的精度和特异性的碱基编辑器
US11512287B2 (en) 2017-06-16 2022-11-29 Sangamo Therapeutics, Inc. Targeted disruption of T cell and/or HLA receptors
WO2019018383A1 (en) 2017-07-18 2019-01-24 Calimmune, Inc. COMPOSITIONS AND METHODS FOR TREATING BETA-HEMOGLOBINOPATHIES
JP2020534795A (ja) 2017-07-28 2020-12-03 プレジデント アンド フェローズ オブ ハーバード カレッジ ファージによって支援される連続的進化（ｐａｃｅ）を用いて塩基編集因子を進化させるための方法および組成物
EP3676376A2 (de) 2017-08-30 2020-07-08 President and Fellows of Harvard College Hocheffiziente baseneditoren mit gam
WO2019079347A1 (en) 2017-10-16 2019-04-25 The Broad Institute, Inc. USES OF BASIC EDITORS ADENOSINE
WO2019094725A2 (en) 2017-11-09 2019-05-16 Sangamo Therapeutics, Inc. Genetic modification of cytokine inducible sh2-containing protein (cish) gene
WO2019143677A1 (en) 2018-01-17 2019-07-25 Vertex Pharmaceuticals Incorporated Quinoxalinone compounds, compositions, methods, and kits for increasing genome editing efficiency
MA51616A (fr) 2018-01-17 2020-11-25 Vertex Pharma Inhibiteurs d'adn-pk
CN111757876B (zh) 2018-01-17 2024-03-22 沃泰克斯药物股份有限公司 Dna-pk抑制剂
US11401512B2 (en) 2018-02-08 2022-08-02 Sangamo Therapeutics, Inc. Engineered target specific nucleases
MX2020010461A (es) 2018-04-05 2021-01-15 Juno Therapeutics Inc Linfocitos t que expresan un receptor recombinante, polinucleotidos y metodos relacionados.
KR20210029707A (ko) 2018-04-05 2021-03-16 주노 쎄러퓨티크스 인코퍼레이티드 재조합 수용체를 발현하는 세포의 생산 방법 및 관련 조성물
CN112313246A (zh) 2018-04-18 2021-02-02 桑格摩生物治疗股份有限公司 用于调节亨廷顿蛋白(htt)的锌指蛋白组合物
US11690921B2 (en) 2018-05-18 2023-07-04 Sangamo Therapeutics, Inc. Delivery of target specific nucleases
CN112867792A (zh) 2018-08-23 2021-05-28 桑格摩生物治疗股份有限公司 工程化靶特异性碱基编辑器
WO2020051283A1 (en) 2018-09-05 2020-03-12 The Regents Of The University Of California Generation of heritably gene-edited plants without tissue culture
AU2019344927A1 (en) 2018-09-18 2021-04-01 Sangamo Therapeutics, Inc. Programmed cell death 1 (PD1) specific nucleases
US20220389395A1 (en) 2018-10-29 2022-12-08 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
WO2020102659A1 (en) 2018-11-15 2020-05-22 The Broad Institute, Inc. G-to-t base editors and uses thereof
CN113166727A (zh) 2018-11-28 2021-07-23 四十七公司 对消融方案耐受的基因修饰的hspc
US11453639B2 (en) 2019-01-11 2022-09-27 Acuitas Therapeutics, Inc. Lipids for lipid nanoparticle delivery of active agents
EP3921417A4 (de) 2019-02-04 2022-11-09 The General Hospital Corporation Varianten von adenin-dna-basen mit reduzierter off-target-rna-editierung
WO2020163017A1 (en) 2019-02-06 2020-08-13 Klogenix Llc Dna binding proteins and uses thereof
WO2020162978A1 (en) 2019-02-06 2020-08-13 Sangamo Therapeutics, Inc. Method for the treatment of mucopolysaccharidosis type i
WO2020181180A1 (en) 2019-03-06 2020-09-10 The Broad Institute, Inc. A:t to c:g base editors and uses thereof
WO2020181178A1 (en) 2019-03-06 2020-09-10 The Broad Institute, Inc. T:a to a:t base editing through thymine alkylation
WO2020181202A1 (en) 2019-03-06 2020-09-10 The Broad Institute, Inc. A:t to t:a base editing through adenine deamination and oxidation
WO2020181195A1 (en) 2019-03-06 2020-09-10 The Broad Institute, Inc. T:a to a:t base editing through adenine excision
WO2020181193A1 (en) 2019-03-06 2020-09-10 The Broad Institute, Inc. T:a to a:t base editing through adenosine methylation
WO2020191249A1 (en) 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
MA55531A (fr) 2019-04-02 2022-02-09 Sangamo Therapeutics Inc Procédés pour le traitement de béta-thalassémie
EP3953485A4 (de) 2019-04-10 2023-05-17 University of Utah Research Foundation Htra1-modulation zur behandlung von amd
EP3956349A1 (de) 2019-04-17 2022-02-23 The Broad Institute, Inc. Adeninbaseneditoren mit reduzierten off-target-effekten
MX2021013219A (es) 2019-05-01 2022-02-17 Juno Therapeutics Inc Celulas que expresan un receptor recombinante de un locus de tgfbr2 modificado, polinucleotidos relacionados y metodos.
AU2020265749A1 (en) 2019-05-01 2022-01-06 Juno Therapeutics, Inc. Cells expressing a chimeric receptor from a modified CD247 locus, related polynucleotides and methods
EP4007596A1 (de) 2019-08-01 2022-06-08 Sana Biotechnology, Inc. Dux4+-exprimierende zellen und ihre verwendungen
WO2021030666A1 (en) 2019-08-15 2021-02-18 The Broad Institute, Inc. Base editing by transglycosylation
JP2022546317A (ja) 2019-08-23 2022-11-04 サナ バイオテクノロジー，インコーポレイテッド Ｃｄ２４発現細胞およびそれらの用途
DK3812472T3 (da) 2019-10-21 2023-02-20 Univ Freiburg Albert Ludwigs Virkelig uvildig in vitro-undersøgelse til profilering af off-target-aktivitet af en eller flere målspecifikke programmerbare nukleaser i celler (abnoba-seq)
TW202132565A (zh) 2019-11-01 2021-09-01 美商聖加莫治療股份有限公司 Ｇｉｎ重組酶變異體
US20230048224A1 (en) 2019-11-01 2023-02-16 Sangamo Therapeutics, Inc. Zinc finger nuclease variants for treating or preventing lysosomal storage diseases
WO2021108717A2 (en) 2019-11-26 2021-06-03 The Broad Institute, Inc Systems and methods for evaluating cas9-independent off-target editing of nucleic acids
WO2021158921A2 (en) 2020-02-05 2021-08-12 The Broad Institute, Inc. Adenine base editors and uses thereof
KR20220158046A (ko) 2020-03-25 2022-11-29 사나 바이오테크놀로지, 인크. 신경계 장애 및 병태의 치료를 위한 면역저하성 신경 세포
US11091524B1 (en) 2020-04-22 2021-08-17 Houston Gene Therapeutics Llc Compositions for treatment of vascular disease
WO2021222318A1 (en) 2020-04-28 2021-11-04 The Broad Institute, Inc. Targeted base editing of the ush2a gene
CA3177621A1 (en) 2020-05-06 2021-11-11 Alexandre Juillerat Methods to genetically modify cells for delivery of therapeutic proteins
EP4146812A1 (de) 2020-05-06 2023-03-15 Cellectis S.A. Verfahren zur gezielten einführung exogener sequenzen in zelluläre genome
CN111549061A (zh) * 2020-05-07 2020-08-18 西南大学 一种高通量筛选真核生物细胞响应环境极端pH的靶点基因的方法
MX2022014008A (es) 2020-05-08 2023-02-09 Broad Inst Inc Métodos y composiciones para la edición simultánea de ambas cadenas de una secuencia de nucleótidos de doble cadena objetivo.
WO2021231661A2 (en) 2020-05-13 2021-11-18 Juno Therapeutics, Inc. Process for producing donor-batched cells expressing a recombinant receptor
WO2021236852A1 (en) 2020-05-20 2021-11-25 Sana Biotechnology, Inc. Methods and compositions for treatment of viral infections
CN116234558A (zh) 2020-06-26 2023-06-06 朱诺治疗学有限公司 条件性地表达重组受体的工程化t细胞、相关多核苷酸和方法
MX2023001831A (es) 2020-08-13 2023-06-29 Sana Biotechnology Inc Métodos de tratamiento de pacientes sensibilizados con células hipoinmunogénicas y métodos y composiciones asociados.
US20220064596A1 (en) 2020-08-25 2022-03-03 Kite Pharma, Inc. T cells with improved functionality
US20230398148A1 (en) 2020-11-04 2023-12-14 Juno Therapeutics, Inc. Cells expressing a chimeric receptor from a modified invariant cd3 immunoglobulin superfamily chain locus and related polynucleotides and methods
CA3200855A1 (en) 2020-11-16 2022-05-19 Pig Improvement Company Uk Limited Influenza a-resistant animals having edited anp32 genes
AU2021412988A1 (en) 2020-12-31 2023-06-15 Sana Biotechnology, Inc. Methods and compositions for modulating car-t activity
JP2024507533A (ja) 2021-02-19 2024-02-20 ビーム・セラピューティクス・インコーポレイテッド 遺伝子治療のための組み換え狂犬病ウイルス
WO2022204155A1 (en) 2021-03-23 2022-09-29 Iovance Biotherapeutics, Inc. Cish gene editing of tumor infiltrating lymphocytes and uses of same in immunotherapy
IL308097A (en) 2021-05-27 2023-12-01 Sana Biotechnology Inc Hypoimmunogenic cells that include transgenic HLA-E or HLA-G
CA3225283A1 (en) 2021-07-14 2023-01-19 Sonja SCHREPFER Altered expression of y chromosome-linked antigens in hypoimmunogenic cells
AU2022325955A1 (en) 2021-08-11 2024-02-08 Sana Biotechnology, Inc. Genetically modified cells for allogeneic cell therapy to reduce instant blood mediated inflammatory reactions
AU2022325231A1 (en) 2021-08-11 2024-02-08 Sana Biotechnology, Inc. Genetically modified cells for allogeneic cell therapy to reduce complement-mediated inflammatory reactions
IL310702A (en) 2021-08-11 2024-04-01 Sana Biotechnology Inc Inducible systems for altering gene expression in hypoimmunogenic cells
WO2023019226A1 (en) 2021-08-11 2023-02-16 Sana Biotechnology, Inc. Genetically modified cells for allogeneic cell therapy
IL310691A (en) 2021-08-11 2024-04-01 Sana Biotechnology Inc Genetically modified primary cells for allogeneic cell therapy
AU2022343725A1 (en) 2021-09-08 2024-03-21 Beam Therapeutics Inc. Viral guide rna delivery
WO2023069790A1 (en) 2021-10-22 2023-04-27 Sana Biotechnology, Inc. Methods of engineering allogeneic t cells with a transgene in a tcr locus and associated compositions and methods
WO2023081756A1 (en) 2021-11-03 2023-05-11 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Precise genome editing using retrons
WO2023081900A1 (en) 2021-11-08 2023-05-11 Juno Therapeutics, Inc. Engineered t cells expressing a recombinant t cell receptor (tcr) and related systems and methods
WO2023105244A1 (en) 2021-12-10 2023-06-15 Pig Improvement Company Uk Limited Editing tmprss2/4 for disease resistance in livestock
WO2023122337A1 (en) 2021-12-23 2023-06-29 Sana Biotechnology, Inc. Chimeric antigen receptor (car) t cells for treating autoimmune disease and associated methods
WO2023141602A2 (en) 2022-01-21 2023-07-27 Renagade Therapeutics Management Inc. Engineered retrons and methods of use
WO2023154578A1 (en) 2022-02-14 2023-08-17 Sana Biotechnology, Inc. Methods of treating patients exhibiting a prior failed therapy with hypoimmunogenic cells
WO2023158836A1 (en) 2022-02-17 2023-08-24 Sana Biotechnology, Inc. Engineered cd47 proteins and uses thereof
WO2023173123A1 (en) 2022-03-11 2023-09-14 Sana Biotechnology, Inc. Genetically modified cells and compositions and uses thereof
WO2023220040A1 (en) 2022-05-09 2023-11-16 Synteny Therapeutics, Inc. Erythroparvovirus with a modified capsid for gene therapy
WO2023220035A1 (en) 2022-05-09 2023-11-16 Synteny Therapeutics, Inc. Erythroparvovirus compositions and methods for gene therapy
WO2023220043A1 (en) 2022-05-09 2023-11-16 Synteny Therapeutics, Inc. Erythroparvovirus with a modified genome for gene therapy
WO2024013514A2 (en) 2022-07-15 2024-01-18 Pig Improvement Company Uk Limited Gene edited livestock animals having coronavirus resistance
WO2024040083A1 (en) 2022-08-16 2024-02-22 The Broad Institute, Inc. Evolved cytosine deaminases and methods of editing dna using same
WO2024044723A1 (en) 2022-08-25 2024-02-29 Renagade Therapeutics Management Inc. Engineered retrons and methods of use

Family Cites Families (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3410650A1 (de) 1984-03-23 1985-10-03 Kernforschungsanlage Jülich GmbH, 5170 Jülich Mit mikroorganismen bewachsene poroese anorganische traeger, verfahren zur immobilisierung von mikroorganismen und dafuer geeignete traegerkoerper
US5455170A (en) 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
US5317090A (en) 1987-12-16 1994-05-31 Institut Pasteur Steroid/thyroid hormone receptor-related gene, which is inappropriately expressed in human hepatocellular carcinoma, and which is a retinoic acid receptor
US5324819A (en) 1988-04-08 1994-06-28 Stryker Corporation Osteogenic proteins
US5340739A (en) 1988-07-13 1994-08-23 Brigham & Women's Hospital Hematopoietic cell specific transcriptional regulatory elements of serglycin and uses thereof
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
JPH04504654A (ja) 1988-12-23 1992-08-20 ザ　ソーク　インスティテュート　フォア　バイオロジカル　スタディーズ レセプターの転写抑制活性組成物および方法
US5198346A (en) 1989-01-06 1993-03-30 Protein Engineering Corp. Generation and selection of novel DNA-binding proteins and polypeptides
US5096815A (en) 1989-01-06 1992-03-17 Protein Engineering Corporation Generation and selection of novel dna-binding proteins and polypeptides
US4990607A (en) 1989-03-14 1991-02-05 The Rockefeller University Alteration of gene expression in plants
CA2047752C (en) 1989-03-17 2001-07-10 Ronald M. Evans Hormone response element compositions and assay
EP0453560B1 (de) 1989-11-13 1999-05-26 Massachusetts Institute Of Technology Lokalisation und charakterisierung des wilms-tumor-gens
US5578482A (en) 1990-05-25 1996-11-26 Georgetown University Ligand growth factors that bind to the erbB-2 receptor protein and induce cellular responses
JPH06503642A (ja) 1990-09-21 1994-04-21 ザ　ソーク　インスティテュート　フォア　バイオロジカル　スタディーズ プロトオンコタンパク質ｃ−ＪＵＮとホルモン受容体との間の機能的拮抗作用
US5173414A (en) 1990-10-30 1992-12-22 Applied Immune Sciences, Inc. Production of recombinant adeno-associated virus vectors
US5348864A (en) 1991-01-25 1994-09-20 E. R. Squibb & Sons, Inc. Mouse vav proto-oncogene DNA and protein sequences
US5871907A (en) 1991-05-15 1999-02-16 Medical Research Council Methods for producing members of specific binding pairs
US5324818A (en) 1991-08-21 1994-06-28 The Regents Of The University Of Michigan Proteins useful in the regulation of κB-containing genes
US5243041A (en) 1991-08-22 1993-09-07 Fernandez Pol Jose A DNA vector with isolated CDNA gene encoding metallopanstimulin
US5302519A (en) 1991-09-09 1994-04-12 Fred Hutchinson Cancer Research Center Method of producing a Mad polypeptide
US5270170A (en) 1991-10-16 1993-12-14 Affymax Technologies N.V. Peptide library and screening method
US5792640A (en) 1992-04-03 1998-08-11 The Johns Hopkins University General method to clone hybrid restriction endonucleases using lig gene
US5356802A (en) 1992-04-03 1994-10-18 The Johns Hopkins University Functional domains in flavobacterium okeanokoites (FokI) restriction endonuclease
US5916794A (en) 1992-04-03 1999-06-29 Johns Hopkins University Methods for inactivating target DNA and for detecting conformational change in a nucleic acid
US5487994A (en) 1992-04-03 1996-01-30 The Johns Hopkins University Insertion and deletion mutants of FokI restriction endonuclease
US5436150A (en) 1992-04-03 1995-07-25 The Johns Hopkins University Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
US5324638A (en) 1992-05-13 1994-06-28 Sloan-Kettering Institute For Cancer Research Brain transcription factor, nucleic acids encoding same and uses thereof
AU4851493A (en) 1992-09-07 1994-03-29 Baylor College Of Medicine Methods and reagents to detect and characterize norwalk and related viruses
US6242568B1 (en) * 1994-01-18 2001-06-05 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
EP0770129B1 (de) 1994-01-18 2005-11-23 The Scripps Research Institute Derivate von zinkfingerproteinen und methoden
US6140466A (en) 1994-01-18 2000-10-31 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
WO1996006110A1 (en) 1994-08-18 1996-02-29 Gilman, Michael, Z. Composite dna-binding proteins and materials and methods relating thereto
GB9824544D0 (en) 1998-11-09 1999-01-06 Medical Res Council Screening system
USRE45795E1 (en) 1994-08-20 2015-11-10 Gendaq, Ltd. Binding proteins for recognition of DNA
DE4435919C1 (de) 1994-10-07 1995-12-07 Deutsches Krebsforsch Zinkfinger-DNA, -Protein und ihre Verwendung
US5871902A (en) 1994-12-09 1999-02-16 The Gene Pool, Inc. Sequence-specific detection of nucleic acid hybrids using a DNA-binding molecule or assembly capable of discriminating perfect hybrids from non-perfect hybrids
US5702914A (en) 1994-12-21 1997-12-30 The Salk Institute For Biological Studies Use of reporter genes for retinoid receptor screening assays having novel retinoid-associated response elements
EP0805819B1 (de) 1994-12-29 2012-02-08 Massachusetts Institute Of Technology Chimäre dna-bindeproteine
US5789538A (en) 1995-02-03 1998-08-04 Massachusetts Institute Of Technology Zinc finger proteins with high affinity new DNA binding specificities
WO1996032475A2 (en) 1995-04-12 1996-10-17 University Of Washington Methods for preparing dna-binding proteins
US5670399A (en) * 1995-12-06 1997-09-23 Micron Technology, Inc. Method of making thin film transistor with offset drain
JP4629165B2 (ja) 1996-01-11 2011-02-09 メトグラス・インコーポレーテッド 分布ギャップ電気チョーク
DE69722176T2 (de) 1996-01-23 2004-03-18 The Board Of Trustees Of The Leland Stanford Junior University, Palo Alto Verfahren zur suche nach transdominanten effektorpeptiden und rna-molekülen
FR2752734B1 (fr) 1996-09-02 1998-11-06 Cird Galderma Utilisation de retinoides pour la preparation d'un medicament destine a traiter les affections liees a une surexpression de vegf
US5939538A (en) 1996-10-25 1999-08-17 Immusol Incorporated Methods and compositions for inhibiting HIV infection of cells by cleaving HIV co-receptor RNA
GB9710807D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
GB9710809D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
WO1999035494A1 (en) * 1998-01-09 1999-07-15 Cubist Pharmaceuticals, Inc. Method for identifying validated target and assay combinations
JP2002508971A (ja) 1998-01-15 2002-03-26 アリアド・ジーン・セラピューティクス・インコーポレーテッド 多量体キメラ蛋白質を使用する生物学的イベントの調節
US5972615A (en) 1998-01-21 1999-10-26 Urocor, Inc. Biomarkers and targets for diagnosis, prognosis and management of prostate disease
US6410248B1 (en) 1998-01-30 2002-06-25 Massachusetts Institute Of Technology General strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites
EP1053317B1 (de) 1998-02-13 2006-11-02 Köster, Hubert Verwendung von ribozymen zur bestimmung der funktion von genen
IL137952A0 (en) 1998-02-20 2001-10-31 Genome Dynamics Inc Method for designing dna-binding proteins of the zinc-finger class
EP1060261B1 (de) * 1998-03-02 2010-05-05 Massachusetts Institute of Technology Poly-zinkfinger-proteine mit verbesserten linkern
AU751487B2 (en) 1998-03-17 2002-08-15 Gendaq Limited Nucleic acid binding proteins
US6140081A (en) 1998-10-16 2000-10-31 The Scripps Research Institute Zinc finger binding domains for GNN
US6453242B1 (en) * 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
US7013219B2 (en) * 1999-01-12 2006-03-14 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US6599692B1 (en) * 1999-09-14 2003-07-29 Sangamo Bioscience, Inc. Functional genomics using zinc finger proteins
US6534261B1 (en) * 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US7070934B2 (en) * 1999-01-12 2006-07-04 Sangamo Biosciences, Inc. Ligand-controlled regulation of endogenous gene expression
US6780590B2 (en) * 1999-09-14 2004-08-24 Sangamo Biosciences, Inc. Gene identification
DE60023936T2 (de) * 1999-12-06 2006-05-24 Sangamo Biosciences Inc., Richmond Methoden zur verwendung von randomisierten zinkfingerprotein-bibliotheken zur identifizierung von genfunktionen

Also Published As

Publication number Publication date
EP1236045A2 (de) 2002-09-04
HK1049515B (zh) 2006-01-13
CA2394850C (en) 2012-02-07
IL150069A (en) 2008-06-05
US7491531B2 (en) 2009-02-17
WO2001040798A3 (en) 2002-06-13
ATE309536T1 (de) 2005-11-15
US20060292621A1 (en) 2006-12-28
US20030134318A1 (en) 2003-07-17
IL187848A0 (en) 2008-03-20
AU776576B2 (en) 2004-09-16
AU2427801A (en) 2001-06-12
US7943553B2 (en) 2011-05-17
US20020146691A1 (en) 2002-10-10
US6503717B2 (en) 2003-01-07
IL150069A0 (en) 2002-12-01
US20060166263A1 (en) 2006-07-27
EP1236045B1 (de) 2005-11-09
DE60023936T2 (de) 2006-05-24
CA2394850A1 (en) 2001-06-07
WO2001040798A2 (en) 2001-06-07
HK1049515A1 (en) 2003-05-16

Similar Documents

Publication Publication Date Title
DE60023936D1 (de) Methoden zur verwendung von randomisierten zinkfingerprotein-bibliotheken zur identifizierung von genfunktionen
ATE313625T1 (de) ßFUNCTIONAL GENOMICSß UNTER VERWENDUNG VON ZINK FINGER PROTEINEN
DE69932813D1 (de) Nukleinsäurebindungsproteine
EP1266025A4 (de) Proteingerüste für antikörper-mimetika und andere bindungsproteine
EA200501772A1 (ru) Способы измерения экспрессии генов из фиксированного и залитого в парафин образца ткани
DE69534629D1 (de) Derivate von zinkfingerproteinen und methoden
DE69809263D1 (de) Methoden ud system zur wahl von datensets
EP1135532A4 (de) Fluoreszierende proteine aus nicht-biolumineszenten arten der klasse anthozoa, gene die diese proteine kodieren und deren verwendungen
ATE295429T1 (de) Two-hybrid-screeningverfahren unter verwendung des bakteriellen transcriptionsaktivators sigma- 54
DE60233147D1 (de) Verwendung von hmgb1 zur aktivierung dendritischer zellen
DE60041452D1 (de) CpG REZEPTOR (CpG-R) UND DARAUF BEZOGENE METHODEN
EP1090294A4 (de) Verfahren um die anwesenheit von krebs in einer probe zu bestimmen durch nachweis der expression eines ssx-gens und peptiden, die von ssx oder ny-eso-1 abgeleitet sind
DE60023860D1 (de) Verfahren zur herstellung von rekombinanten peptiden
TW200700089A (en) Recombinant protein comprising starch binding domain and use thereof
ATE513040T1 (de) Zinkfingerdomänenbibliotheken
DK1082423T3 (da) Cyclin E2-gener og -proteiner
BR9710919A (pt) Proteina purificada sr-p70
ATE288589T1 (de) Verfahren zur analyse von proteinen
ATE230414T1 (de) Mimetika von beta-faltblatt und verfahren zur verwendung davon
ATE285478T1 (de) Zellulosefilme zur durchfuehrung von screening- verfahren
DE60011785D1 (de) Verfahren zur herstellung von proteinen in wirtszellen unter verwendung von chaperoninen
ATE213275T1 (de) Proteinkinase-bestimmung als verfahren zur identifizierung von fungiziden
ATE367581T1 (de) C-TERMINALE REGION DES ßAGOUTI-RELATED- TRANSCRIPTß-PROTEINS (ART)
IL151564A0 (en) Potassium channel proteins
DE602006005932D1 (de) Verfahren zur identifizierung von proteinfaltungsinhibitoren

Legal Events

Date Code Title Description
8364 No opposition during term of opposition