DE60026294D1 - "shot gun" sequenzierung und amplifizierung ohne klonierung - Google Patents

"shot gun" sequenzierung und amplifizierung ohne klonierung

Info

Publication number
DE60026294D1
DE60026294D1 DE60026294T DE60026294T DE60026294D1 DE 60026294 D1 DE60026294 D1 DE 60026294D1 DE 60026294 T DE60026294 T DE 60026294T DE 60026294 T DE60026294 T DE 60026294T DE 60026294 D1 DE60026294 D1 DE 60026294D1
Authority
DE
Germany
Prior art keywords
nucleic acid
sequence
acid template
primer
template
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60026294T
Other languages
English (en)
Other versions
DE60026294T2 (de
Inventor
Periannan Senapathy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genome Technologies LLC
Original Assignee
Genome Technologies LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genome Technologies LLC filed Critical Genome Technologies LLC
Application granted granted Critical
Publication of DE60026294D1 publication Critical patent/DE60026294D1/de
Publication of DE60026294T2 publication Critical patent/DE60026294T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
DE60026294T 1999-04-21 2000-04-20 "shot gun" sequenzierung und amplifizierung ohne klonierung Expired - Lifetime DE60026294T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US434761 1982-10-15
US13035899P 1999-04-21 1999-04-21
US130358P 1999-04-21
US09/434,761 US6521428B1 (en) 1999-04-21 1999-11-04 Shot-gun sequencing and amplification without cloning
PCT/US2000/010745 WO2000063439A2 (en) 1999-04-21 2000-04-20 Shot-gun sequencing and amplification without cloning

Publications (2)

Publication Number Publication Date
DE60026294D1 true DE60026294D1 (de) 2006-04-27
DE60026294T2 DE60026294T2 (de) 2006-12-28

Family

ID=26828421

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60026294T Expired - Lifetime DE60026294T2 (de) 1999-04-21 2000-04-20 "shot gun" sequenzierung und amplifizierung ohne klonierung

Country Status (6)

Country Link
US (3) US6521428B1 (de)
EP (1) EP1183388B1 (de)
AT (1) ATE318931T1 (de)
AU (1) AU4652300A (de)
DE (1) DE60026294T2 (de)
WO (1) WO2000063439A2 (de)

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Also Published As

Publication number Publication date
EP1183388B1 (de) 2006-03-01
US7056706B2 (en) 2006-06-06
EP1183388A2 (de) 2002-03-06
US6521428B1 (en) 2003-02-18
US20010046681A1 (en) 2001-11-29
DE60026294T2 (de) 2006-12-28
AU4652300A (en) 2000-11-02
US6528288B2 (en) 2003-03-04
WO2000063439A2 (en) 2000-10-26
ATE318931T1 (de) 2006-03-15
US20030100006A1 (en) 2003-05-29
WO2000063439A3 (en) 2001-03-29

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