DE60127162D1 - Massives Parallelverfahren zur Dekodierung von DNA und RNA - Google Patents

Massives Parallelverfahren zur Dekodierung von DNA und RNA

Info

Publication number
DE60127162D1
DE60127162D1 DE60127162T DE60127162T DE60127162D1 DE 60127162 D1 DE60127162 D1 DE 60127162D1 DE 60127162 T DE60127162 T DE 60127162T DE 60127162 T DE60127162 T DE 60127162T DE 60127162 D1 DE60127162 D1 DE 60127162D1
Authority
DE
Germany
Prior art keywords
dna
rna
decoding
nucleotide analogue
parallel method
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60127162T
Other languages
English (en)
Other versions
DE60127162T2 (de
Inventor
Yasuhiro Itagaki
Jingyue Ju
Zengmin Li
John Robert Edwards
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Columbia University of New York
Original Assignee
Columbia University of New York
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26972030&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE60127162(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Columbia University of New York filed Critical Columbia University of New York
Publication of DE60127162D1 publication Critical patent/DE60127162D1/de
Application granted granted Critical
Publication of DE60127162T2 publication Critical patent/DE60127162T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/14—Pyrrolo-pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6872—Methods for sequencing involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/117—Modifications characterised by incorporating modified base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/101—Sanger sequencing method, i.e. oligonucleotide sequencing using primer elongation and dideoxynucleotides as chain terminators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/501—Detection characterised by immobilisation to a surface being an array of oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
DE60127162T 2000-10-06 2001-10-05 Massives Parallelverfahren zur Dekodierung von DNA und RNA Expired - Lifetime DE60127162T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US68467000A 2000-10-06 2000-10-06
US684670 2000-10-06
US30089401P 2001-06-26 2001-06-26
US300894P 2001-06-26
PCT/US2001/031243 WO2002029003A2 (en) 2000-10-06 2001-10-05 Massive parallel method for decoding dna and rna

Publications (2)

Publication Number Publication Date
DE60127162D1 true DE60127162D1 (de) 2007-04-19
DE60127162T2 DE60127162T2 (de) 2008-04-24

Family

ID=26972030

Family Applications (2)

Application Number Title Priority Date Filing Date
DE60127162T Expired - Lifetime DE60127162T2 (de) 2000-10-06 2001-10-05 Massives Parallelverfahren zur Dekodierung von DNA und RNA
DE20122767U Expired - Lifetime DE20122767U1 (de) 2000-10-06 2001-10-05 Massives Parallelverfahren zur Dekodierung von DNA und RNA

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE20122767U Expired - Lifetime DE20122767U1 (de) 2000-10-06 2001-10-05 Massives Parallelverfahren zur Dekodierung von DNA und RNA

Country Status (9)

Country Link
US (25) US6664079B2 (de)
EP (3) EP1337541B1 (de)
JP (1) JP2004510433A (de)
AT (1) ATE356222T1 (de)
AU (1) AU2001296645A1 (de)
CA (1) CA2425112C (de)
DE (2) DE60127162T2 (de)
HK (1) HK1227441A1 (de)
WO (1) WO2002029003A2 (de)

Families Citing this family (245)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7153943B2 (en) * 1997-07-14 2006-12-26 Bolder Biotechnology, Inc. Derivatives of growth hormone and related proteins, and methods of use thereof
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7501245B2 (en) * 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US20060057565A1 (en) * 2000-09-11 2006-03-16 Jingyue Ju Combinatorial fluorescence energy transfer tags and uses thereof
US6627748B1 (en) 2000-09-11 2003-09-30 The Trustees Of Columbia University In The City Of New York Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
ATE356222T1 (de) 2000-10-06 2007-03-15 Univ Columbia Massives parallelverfahren zur dekodierung von dna und rna
JP2004523243A (ja) * 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
GB0129012D0 (en) * 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US7166478B2 (en) * 2002-03-12 2007-01-23 Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
AU2003224253A1 (en) 2002-04-04 2003-10-27 Electrophoretics Limited Method for characterising analytes
US7074597B2 (en) * 2002-07-12 2006-07-11 The Trustees Of Columbia University In The City Of New York Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry
HUE032483T2 (en) 2002-08-23 2017-09-28 Illumina Cambridge Ltd Marked nucleotides
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
SI3587433T1 (sl) 2002-08-23 2020-08-31 Illumina Cambridge Limited Modificirani nukleotidi
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
WO2004055160A2 (en) * 2002-12-13 2004-07-01 The Trustees Of Columbia University In The City Of New York Biomolecular coupling methods using 1,3-dipolar cycloaddition chemistry
US7414117B2 (en) * 2002-12-26 2008-08-19 Ngk Insulators, Ltd. Nucleotide derivative and DNA microarray
US7323555B2 (en) * 2002-12-26 2008-01-29 Isao Saito Nucleotide derivative and DNA microarray
EP1725572B1 (de) 2003-11-05 2017-05-31 AGCT GmbH Makromolekulare nukleotidverbindungen und methoden zu deren anwendung
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005080605A2 (en) * 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
WO2005084367A2 (en) * 2004-03-03 2005-09-15 The Trustees Of Columbia University In The City Of New York Photocleavable fluorescent nucleotides for dna sequencing on chip constructed by site-specific coupling chemistry
US7157699B2 (en) * 2004-03-29 2007-01-02 Purdue Research Foundation Multiplexed mass spectrometer
WO2006073436A2 (en) * 2004-04-29 2006-07-13 The Trustees Of Columbia University In The City Of New York Mass tag pcr for multiplex diagnostics
US20070054345A1 (en) 2004-05-19 2007-03-08 Hunter Christie L Expression quantification using mass spectrometry
ATE407939T1 (de) * 2004-06-02 2008-09-15 Asm Scient Inc 2ß-NITROBENZYL-MODIFIZIERTE RIBONUKLEOTIDE
JP2005353340A (ja) * 2004-06-09 2005-12-22 Hitachi Ltd 質量分析装置
US20070048752A1 (en) 2004-07-12 2007-03-01 Applera Corporation Mass tags for quantitative analyses
US20060172319A1 (en) * 2004-07-12 2006-08-03 Applera Corporation Mass tags for quantitative analyses
GB0421294D0 (en) * 2004-09-24 2004-10-27 Angiogene Pharm Ltd Bioreductively-activated prodrugs
EP1816130A4 (de) * 2004-11-08 2012-04-25 Riken Neues nukleosid- oder nukleotidderivat und verwendung davon
EP2272983A1 (de) 2005-02-01 2011-01-12 AB Advanced Genetic Analysis Corporation Reagentien, Verfahren und Bibliotheken zur Sequenzierung mit Kügelchen
EP2241637A1 (de) 2005-02-01 2010-10-20 AB Advanced Genetic Analysis Corporation Nukleinsäuresequenzierung durch schrittweise Duplexverlängerung
US20060183238A1 (en) 2005-02-09 2006-08-17 Applera Corporation Amine-containing compound analysis methods
JP2006241133A (ja) * 2005-03-04 2006-09-14 Univ Of Tokyo 光分解性保護基
US8212020B2 (en) * 2005-03-11 2012-07-03 Steven Albert Benner Reagents for reversibly terminating primer extension
US7544794B1 (en) * 2005-03-11 2009-06-09 Steven Albert Benner Method for sequencing DNA and RNA by synthesis
GB0507835D0 (en) * 2005-04-18 2005-05-25 Solexa Ltd Method and device for nucleic acid sequencing using a planar wave guide
US9169510B2 (en) 2005-06-21 2015-10-27 The Trustees Of Columbia University In The City Of New York Pyrosequencing methods and related compositions
US20070099212A1 (en) * 2005-07-28 2007-05-03 Timothy Harris Consecutive base single molecule sequencing
WO2007021757A2 (en) 2005-08-15 2007-02-22 Massachusetts Institute Of Technology Fluorescent sensor and methods
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7405281B2 (en) * 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
EP1937850B1 (de) 2005-10-27 2019-05-29 The President and Fellows of Harvard College Verfahren und zusammensetzungen zur markierung von nukleinsäuren
WO2007053719A2 (en) * 2005-10-31 2007-05-10 The Trustees Of Columbia University In The City Of New York Chemically cleavable 3'-o-allyl-dntp-allyl-fluorophore fluorescent nucleotide analogues and related methods
WO2007053702A2 (en) * 2005-10-31 2007-05-10 The Trustees Of Columbia University In The City Of New York Synthesis of four color 3'-o-allyl modified photocleavable fluorescent nucleotides and related methods
CA2630544A1 (en) * 2005-11-21 2007-05-31 The Trustees Of Columbia University In The City Of New York Multiplex digital immuno-sensing using a library of photocleavable mass tags
US20070154921A1 (en) * 2005-12-16 2007-07-05 Applera Corporation Method and System for Phase-Locked Sequencing
US8114636B2 (en) 2006-02-10 2012-02-14 Life Technologies Corporation Labeling and detection of nucleic acids
US8716033B2 (en) 2006-02-10 2014-05-06 Life Technologies Corporation Oligosaccharide modification and labeling of proteins
JP2009538123A (ja) * 2006-04-19 2009-11-05 アプライド バイオシステムズ， エルエルシー ゲル非含有ビーズベースの配列決定のための試薬、方法およびライブラリー
WO2007135368A2 (en) 2006-05-18 2007-11-29 Solexa Limited Dye compounds and the use of their labelled conjugates
WO2007146158A1 (en) 2006-06-07 2007-12-21 The Trustees Of Columbia University In The City Of New York Dna sequencing by nanopore using modified nucleotides
WO2008037568A2 (en) * 2006-09-04 2008-04-03 Quiatech Ab Reversible terminators for efficient sequencing by synthesis
US8399188B2 (en) 2006-09-28 2013-03-19 Illumina, Inc. Compositions and methods for nucleotide sequencing
DE112007002932B4 (de) 2006-12-01 2015-08-06 The Trustees Of Columbia University In The City Of New York Vierfarben DNA-Sequenzierung mittels Synthese unter Verwendung von abspaltbaren, reversiblen, fluoreszierenden Nucleotidterminatoren
US7893227B2 (en) * 2006-12-05 2011-02-22 Lasergen, Inc. 3′-OH unblocked nucleotides and nucleosides base modified with non-cleavable, terminating groups and methods for their use in DNA sequencing
BRPI0719923B1 (pt) * 2006-12-05 2021-08-17 Agilent Technologies Inc Métodos para sequenciamento de um ácido nucléico alvo, para conversão de um componente de ocorrência não natural em uma molécula de ácido nucléico em um componente de ocorrência natural, para terminar uma síntese de ácido nucléico, e para realizar o sequenciamento de sanger ou tipo sanger, e compostos
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
US11035823B2 (en) 2009-03-17 2021-06-15 Qiagen Sciences, Llc Methods and devices for sequencing nucleic acids in smaller batches
US8481259B2 (en) 2007-02-05 2013-07-09 Intelligent Bio-Systems, Inc. Methods and devices for sequencing nucleic acids in smaller batches
US11940413B2 (en) 2007-02-05 2024-03-26 IsoPlexis Corporation Methods and devices for sequencing nucleic acids in smaller batches
JP5750209B2 (ja) * 2007-03-16 2015-07-15 アプタ バイオサイエンス リミテッド 機能性分子、機能性分子合成用アミダイド、及び標的物質解析方法
US9163053B2 (en) * 2007-05-18 2015-10-20 Fluidigm Corporation Nucleotide analogs
US20090099027A1 (en) * 2007-08-23 2009-04-16 Applied Biosystems Inc. Methods of Modifying Support Surfaces for the Immobilization of Particles and the Use of the Immobilized Particles for Analyzing Nucleic Acids
US20090062132A1 (en) * 2007-08-29 2009-03-05 Borner Scott R Alternative nucleic acid sequencing methods
EP2212434A1 (de) * 2007-10-01 2010-08-04 Applied Biosystems Inc. Chase-ligation-sequenzierung
DK2725107T3 (da) * 2007-10-19 2019-01-02 Univ Columbia DNA-sekventering med ikke-fluorescerende nukleotidreversible terminatorer og ddNTP'er modificeret med spaltbart mærke og nukleinsyre omfattende inosin med reversible terminatorer
EP2207900B1 (de) 2007-10-19 2015-04-29 The Trustees of Columbia University in the City of New York Konstruktion und synthese spaltbarer fluoreszenznukleotide als reversible terminatoren für die dna-sequenzierung mittels synthese
US20090171078A1 (en) * 2007-11-20 2009-07-02 Applied Biosystems Inc. Method of sequencing nucleic acids using elaborated nucleotide phosphorotiolate compounds
US8017338B2 (en) * 2007-11-20 2011-09-13 Life Technologies Corporation Reversible di-nucleotide terminator sequencing
US8133515B2 (en) 2007-11-21 2012-03-13 University Of Georgia Research Foundation, Inc. Alkynes and methods of reacting alkynes with 1,3-dipole-functional compounds
CA2718404C (en) 2008-03-13 2018-04-10 Pacific Biosciences Of California, Inc. Labeled reactants and their uses
US8623598B2 (en) 2008-03-19 2014-01-07 Intelligent Bio Systems, Inc. Methods and compositions for inhibiting undesired cleaving of labels
US9017973B2 (en) 2008-03-19 2015-04-28 Intelligent Biosystems, Inc. Methods and compositions for incorporating nucleotides
US10745740B2 (en) 2008-03-19 2020-08-18 Qiagen Sciences, Llc Sample preparation
US8058414B2 (en) * 2008-04-29 2011-11-15 Life Technologies Corporation Unnatural polymerase substrates that can sustain enzymatic synthesis of double stranded nucleic acids from a nucleic acid template and methods of use
CN104263816B (zh) 2008-05-16 2018-10-19 生命技术公司 用于测量细胞增殖的双标记法
CA2725239C (en) * 2008-05-27 2019-02-26 Trilink Biotechnologies Chemically modified nucleoside 5'-triphosphates for thermally initiated amplification of nucleic acid
WO2009152353A2 (en) 2008-06-11 2009-12-17 Lasergen, Inc. Nucleotides and nucleosides and methods for their use in dna sequencing
WO2010002939A2 (en) * 2008-06-30 2010-01-07 Life Technologies Corporation Methods for real time single molecule sequencing
US20100120034A1 (en) * 2008-07-03 2010-05-13 Life Technologies Corporation Methylation analysis of mate pairs
US8173198B2 (en) * 2008-07-23 2012-05-08 Life Technologies Corporation Deposition of metal oxides onto surfaces as an immobilization vehicle for carboxylated or phophated particles or polymers
US20100179075A1 (en) * 2008-07-30 2010-07-15 Life Technologies Corporation Particles for use in supported nucleic acid ligation and detection sequencing
EP2340314B8 (de) 2008-10-22 2015-02-18 Illumina, Inc. Erhaltung von informationen in verbindung mit genomischer dna-methylierung
EP2206780B1 (de) 2008-12-23 2012-02-29 Qiagen GmbH Verfahren und Vorrichtung zur Durchführung einer Nukleinsäurepräparation und/oder Amplifikation
KR20100089688A (ko) * 2009-02-04 2010-08-12 삼성전자주식회사 표적핵산의 서열을 분석하는 방법
WO2010111674A2 (en) 2009-03-27 2010-09-30 Life Technologies Corporation Methods and apparatus for single molecule sequencing using energy transfer detection
US20100330569A1 (en) 2009-04-23 2010-12-30 Intelligent Bio-Systems, Inc. Hydroxymethyl Linkers For Labeling Nucleotides
CA2760155A1 (en) * 2009-04-27 2010-11-11 Pacific Biosciences Of California, Inc. Real-time sequencing methods and systems
GB0907372D0 (en) 2009-04-29 2009-06-10 Invitrogen Dynal As Particles
US10174368B2 (en) 2009-09-10 2019-01-08 Centrillion Technology Holdings Corporation Methods and systems for sequencing long nucleic acids
CN102858995B (zh) 2009-09-10 2016-10-26 森特瑞隆技术控股公司 靶向测序方法
US9315860B2 (en) 2009-10-26 2016-04-19 Genovoxx Gmbh Conjugates of nucleotides and method for the application thereof
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
US20110192723A1 (en) * 2010-02-08 2011-08-11 Genia Technologies, Inc. Systems and methods for manipulating a molecule in a nanopore
US9678055B2 (en) 2010-02-08 2017-06-13 Genia Technologies, Inc. Methods for forming a nanopore in a lipid bilayer
US9605307B2 (en) 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US8603741B2 (en) 2010-02-18 2013-12-10 Pacific Biosciences Of California, Inc. Single molecule sequencing with two distinct chemistry steps
US8759037B2 (en) 2010-02-23 2014-06-24 Illumina Cambridge Limited Amplification methods to minimise sequence specific bias
WO2011123246A2 (en) 2010-04-01 2011-10-06 Illumina, Inc. Solid-phase clonal amplification and related methods
US9029103B2 (en) 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
US20120083417A1 (en) * 2010-09-23 2012-04-05 Centrillion Technology Holding Corporation Native-extension parallel sequencing
GB201016484D0 (en) 2010-09-30 2010-11-17 Geneseque As Method
EP2625526B1 (de) 2010-10-04 2017-03-15 Genapsys Inc. Systeme und verfahren für automatisierte und wiederverwendbare parallele biologische reaktionen
WO2013082164A1 (en) 2011-11-28 2013-06-06 Life Technologies Corporation Enhanced ligation reactions
CN107083421A (zh) 2010-12-17 2017-08-22 纽约哥伦比亚大学理事会 使用经修饰的核苷酸和纳米孔检测的dna边合成边测序
WO2012088341A2 (en) 2010-12-22 2012-06-28 Genia Technologies, Inc. Nanopore-based single dna molecule characterization, identification and isolation using speed bumps
US9217132B2 (en) 2011-01-20 2015-12-22 Ibis Biosciences, Inc. Microfluidic transducer
US8962242B2 (en) 2011-01-24 2015-02-24 Genia Technologies, Inc. System for detecting electrical properties of a molecular complex
US9110478B2 (en) 2011-01-27 2015-08-18 Genia Technologies, Inc. Temperature regulation of measurement arrays
US20120252682A1 (en) 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
EP2702171A1 (de) 2011-04-27 2014-03-05 Cherkasov, Dmitry Methoden und komponenten zur detektion von nukleinsäureketten
US20150086981A1 (en) 2011-05-04 2015-03-26 Genovoxx Gmbh Nucleoside-triphosphate conjugate and methods for the use thereof
WO2012162429A2 (en) 2011-05-23 2012-11-29 The Trustees Of Columbia University In The City Of New York Dna sequencing by synthesis using raman and infrared spectroscopy detection
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
MX2014002027A (es) 2011-08-19 2014-11-10 Synthetic Genomics Inc Metodo integrado para identificacion de alto rendimiento de composiciones pesticidas novedosas y uso para el mismo.
WO2013033705A2 (en) * 2011-09-01 2013-03-07 Case Western Reserve University Non-natural nucleosides as theranostic agents
US8889860B2 (en) 2011-09-13 2014-11-18 Lasergen, Inc. 3′-OH unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
US10378051B2 (en) 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
EP2785868B1 (de) 2011-12-01 2017-04-12 Genapsys Inc. Systeme und verfahren für hocheffiziente elektronische sequenzierung und nachweis
US20130244340A1 (en) 2012-01-20 2013-09-19 Genia Technologies, Inc. Nanopore Based Molecular Detection and Sequencing
US8986629B2 (en) 2012-02-27 2015-03-24 Genia Technologies, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
ES2906186T3 (es) 2012-04-09 2022-04-13 Univ Columbia Método para la preparación de nanoporo y usos del mismo
CA2876095A1 (en) 2012-06-15 2013-12-19 Genia Technologies, Inc. Chip set-up and high-accuracy nucleic acid sequencing
EP3674412A1 (de) 2012-06-20 2020-07-01 The Trustees of Columbia University in the City of New York Nukleinsäuresequenzierung durch nanoporendetektion von markierungsmolekülen
US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
US9759711B2 (en) 2013-02-05 2017-09-12 Genia Technologies, Inc. Nanopore arrays
CN103232489B (zh) * 2013-02-18 2016-09-28 常州津坛生物科技有限公司 一种荧光探针化合物及其制备方法和用途
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
US9809852B2 (en) 2013-03-15 2017-11-07 Genapsys, Inc. Systems and methods for biological analysis
US9593373B2 (en) 2013-03-15 2017-03-14 Illumina Cambridge Limited Modified nucleosides or nucleotides
WO2014144898A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Method for detecting multiple predetermined compounds in a sample
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
US11384377B2 (en) 2013-04-02 2022-07-12 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US9279149B2 (en) 2013-04-02 2016-03-08 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
US8808989B1 (en) 2013-04-02 2014-08-19 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
US9771613B2 (en) 2013-04-02 2017-09-26 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acid
US10683536B2 (en) 2013-04-02 2020-06-16 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US11331643B2 (en) 2013-04-02 2022-05-17 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US10337049B2 (en) 2013-06-17 2019-07-02 The Trustees Of Columbia University In The City Of New York Universal methylation profiling methods
US9551697B2 (en) 2013-10-17 2017-01-24 Genia Technologies, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
CN103601779B (zh) * 2013-10-17 2016-05-04 上海交通大学 7-去氮-2’-脱氧-7-卤素取代鸟嘌呤核苷的合成方法
CN103601778A (zh) * 2013-10-17 2014-02-26 上海交通大学 7-去氮-7-取代鸟嘌呤核苷的合成方法
US9322062B2 (en) 2013-10-23 2016-04-26 Genia Technologies, Inc. Process for biosensor well formation
CA2926138A1 (en) 2013-10-23 2015-04-30 Genia Technologies, Inc. High speed molecular sensing with nanopores
CN103588838A (zh) * 2013-10-30 2014-02-19 上海交通大学 碱基修饰核苷酸的合成方法及其用途
CN103819523B (zh) * 2014-01-22 2016-02-10 上海交通大学 7-去氮-7-卤素鸟嘌呤核苷的合成方法
MA39774A (fr) 2014-03-24 2021-05-12 Roche Sequencing Solutions Inc Procédés chimiques pour produire des nucléotides étiquetés
FR3020071B1 (fr) 2014-04-17 2017-12-22 Dna Script Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede
EP3556864B1 (de) 2014-04-18 2020-12-09 Genapsys, Inc. Verfahren und systeme zur nukleinsäureamplifikation
WO2015176070A1 (en) * 2014-05-16 2015-11-19 The Regents Of The University Of California Compositions and methods for single-molecule construction of dna
WO2016022526A1 (en) 2014-08-04 2016-02-11 The Trustees Of The University Of Pennsylvania Ruthenium-based photolinkers and methods of use
GB201413929D0 (en) 2014-08-06 2014-09-17 Geneseque As Method
FR3025201B1 (fr) 2014-09-02 2018-10-12 Dna Script Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques
US10544455B2 (en) 2014-10-03 2020-01-28 Life Technologies Corporation Sequencing methods, compositions and systems using terminator nucleotides
US10487357B2 (en) 2014-10-03 2019-11-26 Life Technologies Corporation Methods of nucleic acid analysis using terminator nucleotides
EP3699283A1 (de) 2014-10-20 2020-08-26 Molecular Assemblies Inc. Modifizierte vorlagenunabhängige enzyme zur polydeoxynukleotidsynthese
WO2016144973A1 (en) 2015-03-09 2016-09-15 The Trustees Of Columbia University In The City Of New York Pore-forming protein conjugate compositions and methods
US9856285B2 (en) * 2015-03-17 2018-01-02 Kaohsiung Medical University Reagents for universal site-specific labeling and modifications of nucleic acids
WO2016201142A1 (en) 2015-06-09 2016-12-15 Life Technologies Corporation Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging
US10077470B2 (en) 2015-07-21 2018-09-18 Omniome, Inc. Nucleic acid sequencing methods and systems
BR112017023418A2 (pt) 2015-07-30 2018-07-24 Illumina, Inc. desbloqueio ortogonal de nucleotídeos
CN114989235A (zh) 2015-09-28 2022-09-02 哥伦比亚大学董事会 用作dna合成测序的可逆终止物的基于新的二硫键接头的核苷酸的设计与合成
WO2017075293A1 (en) 2015-10-28 2017-05-04 Silicon Valley Scientific, Inc. Method and apparatus for encoding cellular spatial position information
US11421264B2 (en) 2015-11-06 2022-08-23 IsoPlexis Corporation Thiol-containing cleave reagents and oxidative wash
KR102454782B1 (ko) 2015-11-06 2022-10-13 퀴아젠 사이언시스, 엘엘씨 뉴클레오티드 유사체
WO2017104398A1 (ja) * 2015-12-17 2017-06-22 株式会社日立ハイテクノロジーズ 生体分子測定装置
US10036011B2 (en) 2016-02-11 2018-07-31 Qiagen Waltham, Inc. Scavenger compounds for improved sequencing-by-synthesis
WO2017139418A1 (en) 2016-02-11 2017-08-17 Qiagen Waltham, Inc. Additive to improve sequencing by synthesis performance
WO2017139419A1 (en) 2016-02-11 2017-08-17 Qiagen Waltham, Inc. Polyphenolic additives in sequencing-by-synthesis
WO2017190018A1 (en) 2016-04-29 2017-11-02 Omniome, Inc. Sequencing method employing ternary complex destabilization to identify cognate nucleotides
EP3463381A4 (de) 2016-05-23 2020-01-01 The Trustees of Columbia University in the City of New York Nukleotidderivate und verfahren zur verwendung davon
US11299769B2 (en) 2016-06-06 2022-04-12 Redvault Biosciences, Lp Target reporter constructs and uses thereof
US10544456B2 (en) 2016-07-20 2020-01-28 Genapsys, Inc. Systems and methods for nucleic acid sequencing
EP4039824A1 (de) 2016-08-15 2022-08-10 Pacific Biosciences of California, Inc. Verfahren und systeme zur sequenzierung von nukleinsäuren
WO2018034780A1 (en) 2016-08-15 2018-02-22 Omniome, Inc. Sequencing method for rapid identification and processing of cognate nucleotide pairs
US11162129B2 (en) 2016-11-09 2021-11-02 IsoPlexis Corporation Photoprotective mixtures as imaging reagents in sequencing-by-synthesis
WO2018125759A1 (en) 2016-12-30 2018-07-05 Omniome, Inc. Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides
WO2018129214A1 (en) 2017-01-04 2018-07-12 Complete Genomics, Inc. Stepwise sequencing by non-labeled reversible terminators or natural nucleotides
CA3050695C (en) 2017-01-20 2024-02-20 Omniome, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
GB201700983D0 (en) 2017-01-20 2017-03-08 Life Tech As Polymeric particles
WO2018165207A1 (en) 2017-03-06 2018-09-13 Singular Genomic Systems, Inc. Nucleic acid sequencing-by-synthesis (sbs) methods that combine sbs cycle steps
US20210285041A1 (en) * 2017-03-28 2021-09-16 The Trustees Of Columbia University In The City Of New York 3'-o-modified nucleotide analogues with different cleavable linkers for attaching fluorescent labels to the base for dna sequencing by synthesis
US9951385B1 (en) 2017-04-25 2018-04-24 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US11542540B2 (en) 2017-06-16 2023-01-03 Life Technologies Corporation Control nucleic acids, and compositions, kits, and uses thereof
US11110114B2 (en) 2017-07-17 2021-09-07 Oxford University Innovation Limited Dinucleotides
US11104700B2 (en) 2017-07-17 2021-08-31 Oxford University Innovation Limited Oligonucleotides
DK3663407T3 (da) 2017-08-01 2023-04-03 Mgi Tech Co Ltd Fremgangsmåde til nukleinsyresekvensering
EP3684955A1 (de) 2017-09-20 2020-07-29 Regeneron Pharmaceuticals, Inc. Immuntherapieverfahren für patienten, deren tumore eine hohe passagiergenmutationsbelastung tragen
SG11202002516WA (en) 2017-09-21 2020-04-29 Genapsys Inc Systems and methods for nucleic acid sequencing
CA3079411C (en) 2017-10-19 2023-12-05 Omniome, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
WO2019079802A1 (en) * 2017-10-20 2019-04-25 President And Fellows Of Harvard College METHODS OF HIGH-RATE ENCODING AND DECODING OF INFORMATION STORED IN DNA
JP7074857B2 (ja) 2017-12-21 2022-05-24 エフ．ホフマン－ラ ロシュ アーゲー 一方向性の核酸配列決定のための組成物及び方法
US10844430B2 (en) 2018-01-24 2020-11-24 Qiagen Sciences, Llc DNA sequencing reaction additive
US20210189460A1 (en) 2018-04-25 2021-06-24 Qiagen Sciences Llc Sequential paired-end sequencing
FI3794012T3 (fi) 2018-05-15 2024-01-08 Illumina Inc Koostumuksia ja menetelmiä pintaan sitoutuneiden oligonukleotidien kemiallista pilkkomista ja suojauksen poistoa varten
US11697736B2 (en) 2018-05-18 2023-07-11 Singular Genomics Systems, Inc. Silicon containing detectable compounds
CN112534063A (zh) 2018-05-22 2021-03-19 安序源有限公司 用于核酸测序的方法、系统和组合物
WO2019232255A1 (en) 2018-05-30 2019-12-05 Dtx Pharma, Inc. Lipid-modified nucleic acid compounds and methods
CN110818757A (zh) * 2018-08-07 2020-02-21 深圳华大生命科学研究院 核苷酸类似物以及筛选dna聚合酶的方法
WO2020056044A1 (en) 2018-09-11 2020-03-19 Singular Genomics Systems, Inc. Modified archaeal family b polymerases
US10738072B1 (en) 2018-10-25 2020-08-11 Singular Genomics Systems, Inc. Nucleotide analogues
EP3878968A4 (de) 2018-11-07 2022-08-17 EGI Tech (Shen Zhen) Co., Limited Verfahren zur sequenzierung von polynukleotiden
US10768173B1 (en) * 2019-09-06 2020-09-08 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
US10704094B1 (en) 2018-11-14 2020-07-07 Element Biosciences, Inc. Multipart reagents having increased avidity for polymerase binding
US10876148B2 (en) 2018-11-14 2020-12-29 Element Biosciences, Inc. De novo surface preparation and uses thereof
SG11202105441WA (en) 2018-12-13 2021-06-29 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
US11293061B2 (en) * 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
JP2022516684A (ja) 2019-01-08 2022-03-01 シンギュラー・ゲノミクス・システムズ・インコーポレイテッド ヌクレオチドの開裂可能なリンカーおよびその使用
EP3924500A1 (de) 2019-02-12 2021-12-22 DNA Script Effiziente produktspaltung in der template-freien enzymatischen synthese von polynukleotiden
CA3130693A1 (en) 2019-02-19 2020-08-27 Ultima Genomics, Inc. Linkers and methods for optical detection and sequencing
EP3988670A4 (de) 2019-05-15 2023-01-25 EGI Tech (Shen Zhen) Co., Limited Einkanaliges sequenzierungsverfahren auf der grundlage von selbstlumineszenz
CN114286867B (zh) 2019-08-20 2024-04-16 青岛华大智造科技有限责任公司 一种基于发光标记物光信号动力学及二次发光信号对多核苷酸进行测序的方法
US11512295B2 (en) 2019-09-12 2022-11-29 Singular Genomics Systems, Inc. Modified thermoccocus polymerases
EP4034669A1 (de) 2019-09-23 2022-08-03 DNA Script Erhöhung der ausbeute von langen sequenzen bei der schablonenfreien enzymatischen synthese von polynukleotiden
US11287422B2 (en) 2019-09-23 2022-03-29 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
EP4055023A4 (de) 2019-11-07 2024-01-03 Singular Genomics Systems Inc Silizium enthaltende nachweisbare verbindungen und verwendungen davon
US11225688B2 (en) 2019-12-23 2022-01-18 Singular Genomics Systems, Inc. Methods for long read sequencing
AU2020416718A1 (en) 2019-12-31 2022-07-07 Singular Genomics Systems, Inc. Polynucleotide barcodes for long read sequencing
US11807851B1 (en) 2020-02-18 2023-11-07 Ultima Genomics, Inc. Modified polynucleotides and uses thereof
US11034942B1 (en) 2020-02-27 2021-06-15 Singular Genomics Systems, Inc. Modified pyrococcus polymerases and uses thereof
WO2021178893A2 (en) 2020-03-06 2021-09-10 Singular Genomics Systems, Inc. Linked paired strand sequencing
WO2021216998A1 (en) 2020-04-24 2021-10-28 Singular Genomics Systems, Inc. Modified nucleotides and uses thereof
WO2021226327A1 (en) * 2020-05-08 2021-11-11 Singular Genomics Systems, Inc. Nucleotide cleavable linkers with rigid spacers and uses thereof
MX2022016492A (es) * 2020-06-22 2023-03-06 Illumina Cambridge Ltd Nucleosidos y nucleotidos con grupo de bloqueo de acetal 3'.
CN115997033A (zh) 2020-06-30 2023-04-21 伊鲁米纳公司 通过合成产生无痕dna的催化控制测序
EP4153606A2 (de) 2020-07-13 2023-03-29 Singular Genomics Systems, Inc. Verfahren zur sequenzierung komplementärer polynukleotide
GB202216718D0 (en) * 2020-10-29 2022-12-21 Ambergen Inc Novel photocleavable mass-tags for multiplexed mass spectrometric imaging of tissues using biomolecular probes
WO2022170212A1 (en) 2021-02-08 2022-08-11 Singular Genomics Systems, Inc. Methods and compositions for sequencing complementary polynucleotides
WO2022232308A1 (en) 2021-04-27 2022-11-03 Singular Genomics Systems, Inc. High density sequencing and multiplexed priming
EP4089098A1 (de) * 2021-05-10 2022-11-16 Miltenyi Biotec B.V. & Co. KG Fret-farbstoffmarkierte reversible nukleotidterminatoren und ihre verwendung bei der sequenzierung von dna
US20230047225A1 (en) 2021-08-14 2023-02-16 Illumina, Inc. Polymerases, compositions, and methods of use
WO2023035110A1 (zh) 2021-09-07 2023-03-16 深圳华大智造科技股份有限公司 一种用于分析靶多核苷酸的序列的方法
WO2023035108A1 (zh) 2021-09-07 2023-03-16 深圳华大智造科技股份有限公司 一种用于分析靶多核苷酸的序列的方法
WO2023107673A2 (en) 2021-12-10 2023-06-15 Singular Genomics Systems, Inc. Cleavable disulfide linkers and uses thereof
CA3222797A1 (en) 2022-03-31 2023-10-05 Ramesh NEELAKANDAN Nucleosides and nucleotides with 3' vinyl blocking group
WO2023232829A1 (en) 2022-05-31 2023-12-07 Illumina, Inc Compositions and methods for nucleic acid sequencing

Family Cites Families (220)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US5175269A (en) 1984-01-30 1992-12-29 Enzo Diagnostics, Inc. Compound and detectable molecules having an oligo- or polynucleotide with modifiable reactive group
US5118605A (en) * 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US4824775A (en) 1985-01-03 1989-04-25 Molecular Diagnostics, Inc. Cells labeled with multiple Fluorophores bound to a nucleic acid carrier
US4772691A (en) 1985-06-05 1988-09-20 The Medical College Of Wisconsin, Inc. Chemically cleavable nucleotides
US4863849A (en) 1985-07-18 1989-09-05 New York Medical College Automatable process for sequencing nucleotide
DE3529478A1 (de) 1985-08-16 1987-02-19 Boehringer Mannheim Gmbh 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung
US4888274A (en) 1985-09-18 1989-12-19 Yale University RecA nucleoprotein filament and methods
CA1340806C (en) 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US5242796A (en) 1986-07-02 1993-09-07 E. I. Du Pont De Nemours And Company Method, system and reagents for DNA sequencing
US5151507A (en) 1986-07-02 1992-09-29 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US5047519A (en) 1986-07-02 1991-09-10 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
GB8810400D0 (en) 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
SE8801070D0 (sv) 1988-03-23 1988-03-23 Pharmacia Ab Method for immobilizing a dna sequence on a solid support
WO1989011548A1 (en) 1988-05-20 1989-11-30 Cetus Corporation Immobilized sequence-specific probes
US5174962A (en) 1988-06-20 1992-12-29 Genomyx, Inc. Apparatus for determining DNA sequences by mass spectrometry
US5043272A (en) 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
GB8910880D0 (en) 1989-05-11 1989-06-28 Amersham Int Plc Sequencing method
US5424186A (en) 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
EP0450060A1 (de) * 1989-10-26 1991-10-09 Sri International Dns-sequenzierung
US5437975A (en) 1991-02-25 1995-08-01 California Institute Of Biological Research Consensus sequence primed polymerase chain reaction method for fingerprinting genomes
JPH04337446A (ja) 1991-05-15 1992-11-25 Hitachi Ltd 微粒子計測方法、定量方法および微粒子計測装置
US5556748A (en) 1991-07-30 1996-09-17 Xenopore Corporation Methods of sandwich hybridization for the quantitative analysis of oligonucleotides
AU2674092A (en) 1991-09-09 1993-04-05 Baylor College Of Medicine Method and device for rapid dna or rna sequencing determination by a base addition sequencing scheme
DE4141178A1 (de) 1991-12-13 1993-06-17 Europ Lab Molekularbiolog Verfahren zur sequenzierung von nukleinsaeuren
DE4141698A1 (de) 1991-12-18 1993-07-01 Bosch Gmbh Robert Verfahren zur schliesszeitregelung
US6555349B1 (en) 1993-01-22 2003-04-29 Cornell Research Foundation, Inc. Methods for amplifying and sequencing nucleic acid molecules using a three component polymerase
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
US5637469A (en) 1992-05-01 1997-06-10 Trustees Of The University Of Pennsylvania Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems
GB9210176D0 (en) 1992-05-12 1992-06-24 Cemu Bioteknik Ab Chemical method
US5383858B1 (en) 1992-08-17 1996-10-29 Medrad Inc Front-loading medical injector and syringe for use therewith
US5436143A (en) 1992-12-23 1995-07-25 Hyman; Edward D. Method for enzymatic synthesis of oligonucleotides
US5516664A (en) 1992-12-23 1996-05-14 Hyman; Edward D. Enzymatic synthesis of repeat regions of oligonucleotides
US6074823A (en) * 1993-03-19 2000-06-13 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
FR2703052B1 (fr) 1993-03-26 1995-06-02 Pasteur Institut Nouvelle méthode de séquençage d'acides nucléiques.
US5959089A (en) 1993-07-19 1999-09-28 Hannessian; Stephen Amino-cyclodextrin syntheses
GB9315847D0 (en) * 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
US5547859A (en) 1993-08-02 1996-08-20 Goodman; Myron F. Chain-terminating nucleotides for DNA sequencing methods
US5789167A (en) 1993-09-10 1998-08-04 Genevue, Inc. Optical detection of position of oligonucleotides on large DNA molecules
US5849542A (en) 1993-11-17 1998-12-15 Amersham Pharmacia Biotech Uk Limited Primer extension mass spectroscopy nucleic acid sequencing method
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5869255A (en) 1994-02-01 1999-02-09 The Regents Of The University Of California Probes labeled with energy transfer couples dyes exemplified with DNA fragment analysis
US6028190A (en) 1994-02-01 2000-02-22 The Regents Of The University Of California Probes labeled with energy transfer coupled dyes
FR2716263B1 (fr) 1994-02-11 1997-01-17 Pasteur Institut Procédé d'alignement de macromolécules par passage d'un ménisque et applications dans un procédé de mise en évidence, séparation et/ou dosage d'une macromolécule dans un échantillon.
US5552278A (en) * 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5714330A (en) 1994-04-04 1998-02-03 Lynx Therapeutics, Inc. DNA sequencing by stepwise ligation and cleavage
US6589736B1 (en) 1994-11-22 2003-07-08 The Trustees Of Boston University Photocleavable agents and conjugates for the detection and isolation of biomolecules
US20020168642A1 (en) 1994-06-06 2002-11-14 Andrzej Drukier Sequencing duplex DNA by mass spectroscopy
US5709999A (en) 1994-08-12 1998-01-20 Myriad Genetics Inc. Linked breast and ovarian cancer susceptibility gene
US5763594A (en) 1994-09-02 1998-06-09 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US5808045A (en) 1994-09-02 1998-09-15 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
US6232465B1 (en) 1994-09-02 2001-05-15 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
US6214987B1 (en) 1994-09-02 2001-04-10 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent formation of phosphodiester bonds using protected nucleotides
US5872244A (en) 1994-09-02 1999-02-16 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US6013445A (en) 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US5614365A (en) 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
DE4438918A1 (de) 1994-11-04 1996-05-09 Hoechst Ag Modifizierte Oligonukleotide, deren Herstellung sowie deren Verwendung
SE9500342D0 (sv) 1995-01-31 1995-01-31 Marek Kwiatkowski Novel chain terminators, the use thereof for nucleic acid sequencing and synthesis and a method of their preparation
WO1996027025A1 (en) 1995-02-27 1996-09-06 Ely Michael Rabani Device, compounds, algorithms, and methods of molecular characterization and manipulation with molecular parallelism
JP3966555B2 (ja) 1995-05-31 2007-08-29 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 熱耐性ｄｎａポリメラーゼ
EP0745686A1 (de) 1995-06-01 1996-12-04 Roche Diagnostics GmbH Die Verwendung der 3'-wesentlichen Bearbeitungs-Wirksamkeit von DNS-Polymerase
US5770365A (en) 1995-08-25 1998-06-23 Tm Technologies, Inc. Nucleic acid capture moieties
US5728528A (en) 1995-09-20 1998-03-17 The Regents Of The University Of California Universal spacer/energy transfer dyes
US5962228A (en) 1995-11-17 1999-10-05 Lynx Therapeutics, Inc. DNA extension and analysis with rolling primers
AU710425B2 (en) 1995-12-18 1999-09-23 Washington University Method for nucleic acid analysis using fluorescence resonance energy transfer
US5658736A (en) 1996-01-16 1997-08-19 Genetics Institute, Inc. Oligonucleotide population preparation
US6312893B1 (en) 1996-01-23 2001-11-06 Qiagen Genomics, Inc. Methods and compositions for determining the sequence of nucleic acid molecules
US6613508B1 (en) 1996-01-23 2003-09-02 Qiagen Genomics, Inc. Methods and compositions for analyzing nucleic acid molecules utilizing sizing techniques
EP0992511B1 (de) 1996-01-23 2009-03-11 Operon Biotechnologies, Inc. Verfahren und Zusammensetzungen zur Bestimmung von Sequenzen von Nukleinsäure-Molekülen
JP2002515738A (ja) 1996-01-23 2002-05-28 アフィメトリックス，インコーポレイティド 核酸分析法
WO1997035033A1 (en) 1996-03-19 1997-09-25 Molecular Tool, Inc. Method for determining the nucleotide sequence of a polynucleotide
IL127560A0 (en) 1996-06-14 1999-10-28 Sarnoff Corp Method for polynucleotide sequencing
US5821356A (en) 1996-08-12 1998-10-13 The Perkin Elmer Corporation Propargylethoxyamino nucleotides
US6361940B1 (en) 1996-09-24 2002-03-26 Qiagen Genomics, Inc. Compositions and methods for enhancing hybridization and priming specificity
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
US5885775A (en) 1996-10-04 1999-03-23 Perseptive Biosystems, Inc. Methods for determining sequences information in polynucleotides using mass spectrometry
US5853992A (en) 1996-10-04 1998-12-29 The Regents Of The University Of California Cyanine dyes with high-absorbance cross section as donor chromophores in energy transfer labels
US5856104A (en) 1996-10-28 1999-01-05 Affymetrix, Inc. Polymorphisms in the glucose-6 phosphate dehydrogenase locus
US5858671A (en) 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
EP0963443B1 (de) 1996-12-10 2006-03-08 Sequenom, Inc. Abspaltbare, nicht-flüchtige moleküle zur massenmarkierung
CA2277545A1 (en) 1997-01-08 1998-07-16 Proligo Llc Bioconjugation of oligonucleotides
US5804386A (en) 1997-01-15 1998-09-08 Incyte Pharmaceuticals, Inc. Sets of labeled energy transfer fluorescent primers and their use in multi component analysis
US6046005A (en) 1997-01-15 2000-04-04 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators comprising a cleavable linking group
US5876936A (en) 1997-01-15 1999-03-02 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators
FR2758822B1 (fr) 1997-01-30 1999-07-02 Centre Nat Rech Scient Utilisation d'un polypeptide a titre de recepteur cellulaire des adenovirus
WO1998033939A1 (fr) 1997-01-31 1998-08-06 Hitachi, Ltd. Procede pour determiner une sequence de base d'acide nucleique et appareil correspondant
US6197557B1 (en) 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
EP0866071B1 (de) 1997-03-20 2004-10-20 F. Hoffmann-La Roche Ag Modifizierte Primer
ATE364718T1 (de) 1997-04-01 2007-07-15 Solexa Ltd Verfahren zur vervielfältigung von nukleinsäure
JP4294740B2 (ja) 1997-05-23 2009-07-15 ソレクサ・インコーポレイテッド 分析物の系列的プロセシングのためのシステムおよび装置
US6309829B1 (en) * 1997-05-27 2001-10-30 Pe Corporation (Ny) Length determination of nucleic acid repeat sequences by discontinuous primer extension
DE69808661T3 (de) 1997-07-28 2012-05-03 Gen-Probe Inc. Sequenzanalyse von nukleinsäuren
US5834203A (en) 1997-08-25 1998-11-10 Applied Spectral Imaging Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardwire therefore
US6008379A (en) 1997-10-01 1999-12-28 The Perkin-Elmer Corporation Aromatic-substituted xanthene dyes
JP2001519538A (ja) 1997-10-10 2001-10-23 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ 核酸アレイのレプリカ増幅
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6227607B1 (en) 1997-10-15 2001-05-08 James E. Dewald, Jr. Latching mechanism for latching a slide out room to main living area
US6627436B2 (en) 1997-10-31 2003-09-30 Stratagene Vector for gene expression in prokaryotic and eukaryotic systems
US5876036A (en) 1997-11-10 1999-03-02 Mathis; Darryl One-on-one basketball game apparatus
US5936087A (en) 1997-11-25 1999-08-10 The Perkin-Elmer Corporation Dibenzorhodamine dyes
GB9815163D0 (en) 1998-07-13 1998-09-09 Brax Genomics Ltd Compounds
WO1999049082A2 (en) 1998-03-23 1999-09-30 Invitrogen Corporation Modified nucleotides and methods useful for nucleic acid sequencing
EP1082458A1 (de) 1998-05-01 2001-03-14 Arizona Board Of Regents Verfahren zur bestimmung der nukleotidsequenz von oligonukleotiden und dns molekülen
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6175107B1 (en) 1998-05-27 2001-01-16 Owens-Brockway Glass Container Inc. Inspection of containers employing a single area array sensor and alternately strobed light sources
US6096875A (en) 1998-05-29 2000-08-01 The Perlein-Elmer Corporation Nucleotide compounds including a rigid linker
US5948648A (en) 1998-05-29 1999-09-07 Khan; Shaheer H. Nucleotide compounds including a rigid linker
US6218530B1 (en) 1998-06-02 2001-04-17 Ambergen Inc. Compounds and methods for detecting biomolecules
US6287821B1 (en) 1998-06-11 2001-09-11 Orchid Biosciences, Inc. Nucleotide analogues with 3'-pro-fluorescent fluorophores in nucleic acid sequence analysis
US6335155B1 (en) 1998-06-26 2002-01-01 Sunesis Pharmaceuticals, Inc. Methods for rapidly identifying small organic molecule ligands for binding to biological target molecules
US6218118B1 (en) 1998-07-09 2001-04-17 Agilent Technologies, Inc. Method and mixture reagents for analyzing the nucleotide sequence of nucleic acids by mass spectrometry
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
CA2339121A1 (en) * 1998-07-30 2000-02-10 Shankar Balasubramanian Arrayed biomolecules and their use in sequencing
GB0002310D0 (en) 2000-02-01 2000-03-22 Solexa Ltd Polynucleotide sequencing
WO2000009753A1 (en) 1998-08-11 2000-02-24 Caliper Technologies Corp. Methods and systems for sequencing dna by distinguishing the decay times of fluorescent probes
US6245507B1 (en) 1998-08-18 2001-06-12 Orchid Biosciences, Inc. In-line complete hyperspectral fluorescent imaging of nucleic acid molecules
US20020012913A1 (en) 1998-09-15 2002-01-31 Kevin L. Gunderson Nucleic acid analysis using complete n-mer arrays
DE19844931C1 (de) 1998-09-30 2000-06-15 Stefan Seeger Verfahren zur DNS- oder RNS-Sequenzierung
US6500650B1 (en) 1998-10-01 2002-12-31 Variagenics, Inc. Method for identifying polymorphisms
US6221592B1 (en) 1998-10-20 2001-04-24 Wisconsin Alumi Research Foundation Computer-based methods and systems for sequencing of individual nucleic acid molecules
AU2180200A (en) 1998-12-14 2000-07-03 Li-Cor Inc. A heterogeneous assay for pyrophosphate detection
US6207831B1 (en) * 1998-12-21 2001-03-27 Novartis Ag Fluorescent dyes (AIDA) for solid phase and solution phase screening
US6380378B1 (en) 1998-12-24 2002-04-30 Toagosei Company, Ltd. Nucleotide compound, nucleotide block oligonucleotide, and method for producing them
AU2496900A (en) 1999-01-06 2000-07-24 Hyseq, Inc. Enhanced sequencing by hybridization using pools of probes
US20030054360A1 (en) 1999-01-19 2003-03-20 Larry Gold Method and apparatus for the automated generation of nucleic acid ligands
EP1163052B1 (de) 1999-02-23 2010-06-02 Caliper Life Sciences, Inc. Manipulation von mikroteilchen in mikrofluiden systemen
DK1159453T3 (da) 1999-03-10 2008-10-06 Asm Scient Inc Fremgangsmåde til direkte nukleinsyresekventering
US7037654B2 (en) 1999-04-30 2006-05-02 Aclara Biosciences, Inc. Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6277607B1 (en) 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
US6248884B1 (en) 1999-06-03 2001-06-19 The Perkin-Elmer Corporation Extended rhodamine compounds useful as fluorescent labels
DE60019512T2 (de) 1999-06-07 2006-02-23 Fuji Photo Film Co., Ltd., Minami-Ashigara DNA Chip, PNA Chip, sowie Herstellungsverfahren
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6242193B1 (en) 1999-07-30 2001-06-05 Hitachi, Ltd. Apparatus for determining base sequence of nucleic acid
US6316230B1 (en) 1999-08-13 2001-11-13 Applera Corporation Polymerase extension at 3′ terminus of PNA-DNA chimera
CA2385475A1 (en) 1999-08-16 2001-02-22 Human Genome Sciences, Inc. 18 human secreted proteins
US6982146B1 (en) 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
WO2001016375A2 (en) 1999-08-30 2001-03-08 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
US6664399B1 (en) 1999-09-02 2003-12-16 E. I. Du Pont De Nemours & Company Triazole linked carbohydrates
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
WO2001023610A2 (en) 1999-09-29 2001-04-05 Solexa Ltd. Polynucleotide sequencing
US6309836B1 (en) 1999-10-05 2001-10-30 Marek Kwiatkowski Compounds for protecting hydroxyls and methods for their use
CA2387380C (en) 1999-10-08 2011-08-02 Robert C. Leif Conjugated polymer tag complexes
AU1047901A (en) 1999-10-22 2001-04-30 Genset Methods of genetic cluster analysis and use thereof
WO2001032930A1 (en) 1999-11-04 2001-05-10 California Institute Of Technology Methods and apparatuses for analyzing polynucleotide sequences
WO2001048184A2 (de) 1999-12-23 2001-07-05 Axaron Bioscience Ag Verfahren zur parallelen sequenzierung eines nukleinsäuregemisches an einer oberfläche
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
US7037684B2 (en) 2000-03-14 2006-05-02 Itoham Foods Inc. Process for producing polypeptide having disulfide bond
US6495680B1 (en) 2000-03-24 2002-12-17 The University Of Toledo Helices and nanotubes on folding compositions and method of making same
EP1182267B1 (de) 2000-03-30 2012-01-18 Toyota Jidosha Kabushiki Kaisha Verfahren zur bestimmung der basensequenz eines einzelnen nukleinsäuremoleküls
GB0013276D0 (en) 2000-06-01 2000-07-26 Amersham Pharm Biotech Uk Ltd Nucleotide analogues
GB0016473D0 (en) 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method
ATE384731T1 (de) 2000-08-03 2008-02-15 Hoffmann La Roche Nukleinsäurebindende verbindungen mit pyrazolo 3, 4-d pyrimidinanalogen von purin-2,6-diamin und ihre verwendung
US20060057565A1 (en) 2000-09-11 2006-03-16 Jingyue Ju Combinatorial fluorescence energy transfer tags and uses thereof
WO2002022883A1 (en) 2000-09-11 2002-03-21 The Trustees Of Columbia University In The City Of New York Combinatorial fluorescence energy transfer tags and uses thereof
US6627748B1 (en) 2000-09-11 2003-09-30 The Trustees Of Columbia University In The City Of New York Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
ATE356222T1 (de) 2000-10-06 2007-03-15 Univ Columbia Massives parallelverfahren zur dekodierung von dna und rna
JP2004523243A (ja) 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
US20030027140A1 (en) 2001-03-30 2003-02-06 Jingyue Ju High-fidelity DNA sequencing using solid phase capturable dideoxynucleotides and mass spectrometry
DE10120797B4 (de) 2001-04-27 2005-12-22 Genovoxx Gmbh Verfahren zur Analyse von Nukleinsäureketten
DE10120798B4 (de) 2001-04-27 2005-12-29 Genovoxx Gmbh Verfahren zur Bestimmung der Genexpression
US6573677B2 (en) * 2001-06-18 2003-06-03 Motorola, Inc. Method of compensating for abrupt load changes in an anti-pinch window control system
US6613523B2 (en) 2001-06-29 2003-09-02 Agilent Technologies, Inc. Method of DNA sequencing using cleavable tags
US7057031B2 (en) 2001-07-13 2006-06-06 Ambergen, Inc. Nucleotide compositions comprising photocleavable markers and methods of preparation thereof
US6902904B2 (en) 2001-08-27 2005-06-07 Pharmanetics Incorporated Coagulation assay reagents containing lanthanides
AU2002337030A1 (en) 2001-08-29 2003-03-18 Genovoxx Gmbh Method for analyzing nucleic acid sequences and gene expression
GB0128526D0 (en) 2001-11-29 2002-01-23 Amersham Pharm Biotech Uk Ltd Nucleotide analogues
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US20030166282A1 (en) 2002-02-01 2003-09-04 David Brown High potency siRNAS for reducing the expression of target genes
US20030180769A1 (en) 2002-02-05 2003-09-25 Metzker Michael L. Substituted 4,4-difluoro-4-bora-3A,4A-diaza-s-indacene compounds for 8-color DNA sequencing
US6858393B1 (en) 2002-03-13 2005-02-22 Stratagene California Chain terminators for DNA synthesis
US20050244827A1 (en) 2002-04-04 2005-11-03 Charlotta Olsson Method
EP1497304B1 (de) 2002-04-12 2014-06-25 Catalyst Assets LLC Doppelt markierte nukleotide
US7074597B2 (en) 2002-07-12 2006-07-11 The Trustees Of Columbia University In The City Of New York Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry
ATE322500T1 (de) 2002-08-12 2006-04-15 Hoffmann La Roche Verfahren zur herstellung von einem ribofuranose
HUE032483T2 (en) 2002-08-23 2017-09-28 Illumina Cambridge Ltd Marked nucleotides
SI3587433T1 (sl) 2002-08-23 2020-08-31 Illumina Cambridge Limited Modificirani nukleotidi
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
JP2004085934A (ja) * 2002-08-27 2004-03-18 Pentax Corp ズームレンズ鏡筒の繰出カム機構及び繰出カム機構
WO2004055160A2 (en) 2002-12-13 2004-07-01 The Trustees Of Columbia University In The City Of New York Biomolecular coupling methods using 1,3-dipolar cycloaddition chemistry
WO2004096747A1 (en) 2003-04-28 2004-11-11 Basf Aktiengesellschaft Process for the separation of palladium catalyst from crude reaction mixtures of aryl acetic acids obtained by carbonylation
DE10324808B4 (de) 2003-06-02 2005-10-20 Bosch Gmbh Robert Optimierte Steuergerät-Konfiguration für eine Kfz-Feststellbremse
US20050170367A1 (en) 2003-06-10 2005-08-04 Quake Stephen R. Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
US7622026B2 (en) * 2004-03-02 2009-11-24 Panasonic Corporation Biosensor
WO2005084367A2 (en) 2004-03-03 2005-09-15 The Trustees Of Columbia University In The City Of New York Photocleavable fluorescent nucleotides for dna sequencing on chip constructed by site-specific coupling chemistry
US20050239134A1 (en) 2004-04-21 2005-10-27 Board Of Regents, The University Of Texas System Combinatorial selection of phosphorothioate single-stranded DNA aptamers for TGF-beta-1 protein
WO2006073436A2 (en) 2004-04-29 2006-07-13 The Trustees Of Columbia University In The City Of New York Mass tag pcr for multiplex diagnostics
US20060105461A1 (en) 2004-10-22 2006-05-18 May Tom-Moy Nanopore analysis system
US7393533B1 (en) 2004-11-08 2008-07-01 La Jolla Institute For Allergy And Immunology H3L envelope protein immunization methods and H3L envelope passive protection methods
US9169510B2 (en) 2005-06-21 2015-10-27 The Trustees Of Columbia University In The City Of New York Pyrosequencing methods and related compositions
WO2007053702A2 (en) 2005-10-31 2007-05-10 The Trustees Of Columbia University In The City Of New York Synthesis of four color 3'-o-allyl modified photocleavable fluorescent nucleotides and related methods
WO2007053719A2 (en) 2005-10-31 2007-05-10 The Trustees Of Columbia University In The City Of New York Chemically cleavable 3'-o-allyl-dntp-allyl-fluorophore fluorescent nucleotide analogues and related methods
CA2630544A1 (en) 2005-11-21 2007-05-31 The Trustees Of Columbia University In The City Of New York Multiplex digital immuno-sensing using a library of photocleavable mass tags
WO2007146158A1 (en) 2006-06-07 2007-12-21 The Trustees Of Columbia University In The City Of New York Dna sequencing by nanopore using modified nucleotides
US8399188B2 (en) 2006-09-28 2013-03-19 Illumina, Inc. Compositions and methods for nucleotide sequencing
DE112007002932B4 (de) 2006-12-01 2015-08-06 The Trustees Of Columbia University In The City Of New York Vierfarben DNA-Sequenzierung mittels Synthese unter Verwendung von abspaltbaren, reversiblen, fluoreszierenden Nucleotidterminatoren
US8481259B2 (en) 2007-02-05 2013-07-09 Intelligent Bio-Systems, Inc. Methods and devices for sequencing nucleic acids in smaller batches
EP2207900B1 (de) 2007-10-19 2015-04-29 The Trustees of Columbia University in the City of New York Konstruktion und synthese spaltbarer fluoreszenznukleotide als reversible terminatoren für die dna-sequenzierung mittels synthese
DK2725107T3 (da) 2007-10-19 2019-01-02 Univ Columbia DNA-sekventering med ikke-fluorescerende nukleotidreversible terminatorer og ddNTP'er modificeret med spaltbart mærke og nukleinsyre omfattende inosin med reversible terminatorer
JP5018402B2 (ja) 2007-10-31 2012-09-05 ダイキン工業株式会社 調湿装置
US8623598B2 (en) 2008-03-19 2014-01-07 Intelligent Bio Systems, Inc. Methods and compositions for inhibiting undesired cleaving of labels
DE102009027275A1 (de) 2009-06-29 2010-12-30 Robert Bosch Gmbh Bildverarbeitungsverfahren für ein Fahrerassistenzsystem eines Kraftfahrzeugs zur Detektion und Klassifikation wenigstens eines Teils wenigstens eines vorgegebenen Bildelements
US9309569B2 (en) 2010-08-26 2016-04-12 Intelligent Bio-Systems, Inc. Methods and compositions for sequencing nucleic acid using charge
CN107083421A (zh) 2010-12-17 2017-08-22 纽约哥伦比亚大学理事会 使用经修饰的核苷酸和纳米孔检测的dna边合成边测序
WO2012162429A2 (en) 2011-05-23 2012-11-29 The Trustees Of Columbia University In The City Of New York Dna sequencing by synthesis using raman and infrared spectroscopy detection
ES2906186T3 (es) 2012-04-09 2022-04-13 Univ Columbia Método para la preparación de nanoporo y usos del mismo
EP3674412A1 (de) 2012-06-20 2020-07-01 The Trustees of Columbia University in the City of New York Nukleinsäuresequenzierung durch nanoporendetektion von markierungsmolekülen
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
WO2014144898A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Method for detecting multiple predetermined compounds in a sample
US9159610B2 (en) 2013-10-23 2015-10-13 Globalfoundires, Inc. Hybrid manganese and manganese nitride barriers for back-end-of-line metallization and methods for fabricating the same
EP3105354B1 (de) 2014-02-12 2020-05-06 The Trustees of Columbia University in the City of New York Elektronische einzelmolekül-multiplex-snp-assays und -pcr-analysen
MA39774A (fr) 2014-03-24 2021-05-12 Roche Sequencing Solutions Inc Procédés chimiques pour produire des nucléotides étiquetés
WO2015179284A1 (en) 2014-05-19 2015-11-26 The Trustees Of Columbia University In The City Of New York Ion sensor dna and rna sequencing by synthesis using nucleotide reversible terminators
US10113084B2 (en) 2014-05-22 2018-10-30 Illinois Tool Works, Inc. Mold release agent

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