DE60222427D1 - Puffer-arrays zum nachweis von biomolekülen anhand ihres isoelektrischen punktes - Google Patents
Puffer-arrays zum nachweis von biomolekülen anhand ihres isoelektrischen punktesInfo
- Publication number
- DE60222427D1 DE60222427D1 DE60222427T DE60222427T DE60222427D1 DE 60222427 D1 DE60222427 D1 DE 60222427D1 DE 60222427 T DE60222427 T DE 60222427T DE 60222427 T DE60222427 T DE 60222427T DE 60222427 D1 DE60222427 D1 DE 60222427D1
- Authority
- DE
- Germany
- Prior art keywords
- isoelectric point
- detection
- arrays
- buffer arrays
- biomolecules based
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44795—Isoelectric focusing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
Applications Claiming Priority (9)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US30580201P | 2001-07-16 | 2001-07-16 | |
US305802P | 2001-07-16 | ||
US31031601P | 2001-08-06 | 2001-08-06 | |
US310316P | 2001-08-06 | ||
US34069801P | 2001-10-29 | 2001-10-29 | |
US340698P | 2001-10-29 | ||
US37704402P | 2002-04-30 | 2002-04-30 | |
US377044P | 2002-04-30 | ||
PCT/US2002/022714 WO2003008977A2 (en) | 2001-07-16 | 2002-07-16 | Arrays of buffers for analysing biomolecules by their isoelectric point |
Publications (2)
Publication Number | Publication Date |
---|---|
DE60222427D1 true DE60222427D1 (de) | 2007-10-25 |
DE60222427T2 DE60222427T2 (de) | 2008-06-12 |
Family
ID=27501888
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60222427T Expired - Lifetime DE60222427T2 (de) | 2001-07-16 | 2002-07-16 | Puffer-arrays zum nachweis von biomolekülen anhand ihres isoelektrischen punktes |
Country Status (12)
Country | Link |
---|---|
US (2) | US7166202B2 (de) |
EP (1) | EP1410029B1 (de) |
JP (1) | JP4108037B2 (de) |
CN (1) | CN1549924A (de) |
AT (1) | ATE373236T1 (de) |
AU (1) | AU2002322513B2 (de) |
BR (1) | BR0211213A (de) |
CA (1) | CA2454045A1 (de) |
DE (1) | DE60222427T2 (de) |
DK (1) | DK1410029T3 (de) |
IL (1) | IL159810A0 (de) |
WO (1) | WO2003008977A2 (de) |
Families Citing this family (38)
Publication number | Priority date | Publication date | Assignee | Title |
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IL159810A0 (en) * | 2001-07-16 | 2004-06-20 | Protein Forest Inc | Matrixes, arrays, systems and methods |
US7622028B2 (en) | 2003-05-09 | 2009-11-24 | Life Technologies Corporation | Solution phase electrophoresis device, components, and methods |
WO2004104548A2 (en) | 2003-05-09 | 2004-12-02 | Invitrogen Corporation | Solution phase electrophoresis device, components, and methods |
WO2005021841A2 (en) | 2003-09-03 | 2005-03-10 | Bioforms | Methods and apparatus for rapid crystallization of biomolecules |
WO2005029060A1 (en) * | 2003-09-24 | 2005-03-31 | Agilent Technologies, Inc. | Electrophoretic separation of amphoteric molecules |
WO2005047881A2 (en) * | 2003-11-05 | 2005-05-26 | Exact Sciences Corporation | Repetitive reversed-field affinity electrophoresis and uses therefor |
WO2005089910A1 (en) * | 2004-03-17 | 2005-09-29 | Ciphergen Biosystems, Inc. | Multi-compartment filter and method of filtering using same |
JP4644610B2 (ja) * | 2006-02-17 | 2011-03-02 | 京都市 | 等電点電気泳動装置 |
US20070209939A1 (en) * | 2006-03-10 | 2007-09-13 | Protein Forest, Inc. | Two-dimensional transfer device |
EP2049260B1 (de) | 2006-08-03 | 2018-09-26 | Agilent Technologies, Inc. | Kanalfreies transportmedium für fluidprobe |
CN103499628A (zh) * | 2006-11-21 | 2014-01-08 | 麦迪美特控股有限公司 | 用于液体的离子传感器及其制造方法 |
EA201201363A1 (ru) * | 2006-11-21 | 2013-08-30 | Медимейт Холдинг Б.В. | Датчик ионов для текучей среды и способ его изготовления |
EP2105735B1 (de) * | 2006-12-26 | 2014-11-26 | Sekisui Chemical Co., Ltd. | VERFAHREN ZUR MESSUNG VON STABILEM HÄMOGLOBIN A1c UND ELEKTROPHORESEGERÄT |
US20080272002A1 (en) * | 2007-05-03 | 2008-11-06 | Protein Forest, Inc. | System and Method for Proteomics |
GB2452239B (en) * | 2007-06-01 | 2012-08-29 | Kratos Analytical Ltd | Method and apparatus useful for imaging |
JP2009042004A (ja) * | 2007-08-07 | 2009-02-26 | Norio Okuyama | 電気泳動用支持体 |
EP2193100B1 (de) | 2007-08-27 | 2014-05-07 | Technion Research and Development Foundation, Limited | Durch Elektrolyse gesteuerte pH-Gradienten und ihre Verwendung bei der isoelektrischen Fokussierung |
US20090110833A1 (en) * | 2007-10-31 | 2009-04-30 | Gala Industries, Inc. | Method for abrasion-resistant non-stick surface treatments for pelletization and drying process equipment components |
US9032441B2 (en) * | 2007-12-21 | 2015-05-12 | BrightTALK Limited | System and method for self management of a live web event |
WO2009102978A2 (en) * | 2008-02-14 | 2009-08-20 | University Of Notre Dame Du Lac | Ac field induced biomolecule crystallization and hydration cage disruption |
EP2146200A1 (de) * | 2008-07-16 | 2010-01-20 | Agilent Technologies, Inc. | Vorrichtung und Verfahren zur isoelektrischen Fokussierung |
WO2010023589A1 (en) * | 2008-08-28 | 2010-03-04 | Koninklijke Philips Electronics N.V. | Method for preparing a ph gradient in an isoelectric focusing biochip |
CN101556260B (zh) * | 2009-05-25 | 2012-01-11 | 北京理工大学 | 固定化pH梯度毛细管等电聚焦测定微生物等电点的方法 |
US9182411B2 (en) * | 2009-07-14 | 2015-11-10 | Colen Innovations, L.L.C. | Methods and apparatus for the detection and differentiation of non-sialated proteins from sialated proteins in a fluid sample |
CN102483390B (zh) | 2009-08-18 | 2014-11-19 | 工业研究与发展基金会有限公司 | 质子浓度形貌、用于产生其的方法和装置 |
CA2770518A1 (en) | 2009-08-18 | 2011-02-24 | Technion Research & Development Foundation Ltd. | Methods and devices of separating molecular analytes |
KR101431769B1 (ko) * | 2009-12-10 | 2014-08-20 | 삼성전자주식회사 | 당화 혈색소 측정용 원심력 기반의 미세유동 구조물, 당화 혈색소 측정용 원심력 기반 미세유동 장치 및 당화 혈색소의 측정방법 |
US20120190129A1 (en) * | 2011-01-20 | 2012-07-26 | Konica Minolta Holdings, Inc. | Test device, reaction apparatus and reactive test method |
EP2747879B1 (de) * | 2011-08-23 | 2019-12-18 | Board Of Regents, The University Of Texas System | Drei-elektroden-puffergenerator und verfahren dafür |
US8917096B2 (en) | 2012-11-15 | 2014-12-23 | International Business Machines Corporation | Determination of isoelectric points of biomolecules using capacitive sensors |
US9250206B2 (en) | 2013-04-04 | 2016-02-02 | International Business Machines Corporation | Controlled translocation of macromolecules employing a funnel nanopore structure and a gel |
CN103412029B (zh) * | 2013-06-26 | 2018-10-16 | 华东理工大学 | 基于芯片级别的平板电层析氨基酸分离装置及其使用方法 |
EP3145625B1 (de) * | 2014-05-21 | 2023-07-05 | Unchained Labs | Systeme und verfahren für den austausch von pufferlösungen |
AU2016365121B2 (en) | 2015-11-30 | 2022-03-03 | Intabio, Inc. | Devices and methods for sample characterization |
EP3746211A4 (de) | 2018-01-29 | 2021-10-27 | Intabio, Inc. | Vorrichtungen, verfahren und kits zur probencharakterisierung |
SG11202011729UA (en) | 2018-05-31 | 2020-12-30 | Intabio Inc | Software for microfluidic systems interfacing with mass spectrometry |
CN112354572B (zh) * | 2019-07-11 | 2022-02-11 | 北京理工大学 | 一种多维微流控电泳芯片及检测装置,检测方法 |
US11285484B2 (en) | 2019-08-12 | 2022-03-29 | Intabio, Llc | Multichannel isoelectric focusing devices and high voltage power supplies |
Family Cites Families (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2861803D1 (en) | 1977-06-15 | 1982-07-01 | Nat Res Dev | Electrophoresis membranes, their use in a separation method and separation apparatus |
US4473452A (en) | 1982-11-18 | 1984-09-25 | The Trustees Of Columbia University In The City Of New York | Electrophoresis using alternating transverse electric fields |
EP0287513B1 (de) | 1987-04-11 | 1992-12-02 | Ciba-Geigy Ag | Isoelektrisches Fokussierverfahren sowie Einrichtung zur Durchführung dieses Verfahrens |
US5114555A (en) | 1988-01-05 | 1992-05-19 | Monsanto Company | Continuous isoelectric separation |
US4900414A (en) * | 1988-08-19 | 1990-02-13 | Drug Delivery Systems Inc. | Commercial separation system and method using electrokinetic techniques |
US5160594A (en) * | 1989-03-08 | 1992-11-03 | Board Of Regents Of The University Of Texas System | Apparatus and methods for isoelectric focusing of amphoteric substances incorporating ion selective membranes in electrode chambers |
GB9007922D0 (en) * | 1990-04-07 | 1990-06-06 | Oxford Virology Plc | A diagnostic test method |
US5336387A (en) | 1990-09-11 | 1994-08-09 | Bioseparations, Inc. | Electrical separator apparatus and method of counterflow gradient focusing |
US6017696A (en) * | 1993-11-01 | 2000-01-25 | Nanogen, Inc. | Methods for electronic stringency control for molecular biological analysis and diagnostics |
JP2578285B2 (ja) * | 1992-02-28 | 1997-02-05 | 矢崎総業株式会社 | 防水コネクタ |
US5376249A (en) | 1992-11-25 | 1994-12-27 | Perseptive Biosystems, Inc. | Analysis utilizing isoelectric focusing |
US5571410A (en) | 1994-10-19 | 1996-11-05 | Hewlett Packard Company | Fully integrated miniaturized planar liquid sample handling and analysis device |
US5630924A (en) | 1995-04-20 | 1997-05-20 | Perseptive Biosystems, Inc. | Compositions, methods and apparatus for ultrafast electroseparation analysis |
GB9509410D0 (en) | 1995-05-10 | 1995-07-05 | Imperial College | Molecular imaging |
IT1282607B1 (it) | 1996-02-13 | 1998-03-31 | Pier Giorgio Righetti | Uso di gradienti multipli per l'analisi di mutazioni in dna genomico umano |
WO1998013689A1 (de) * | 1996-09-28 | 1998-04-02 | Fuhr Guenter | Verfahren und vorrichtung zur isoelektrischen teilchentrennung |
US5885430A (en) | 1996-10-04 | 1999-03-23 | Spectrumedix Corporation | Capillary tube holder for an electrophoretic apparatus |
US5773645A (en) | 1997-05-05 | 1998-06-30 | Bio-Rad Laboratories, Inc. | Two-dimensional electrophoresis device |
US5993627A (en) | 1997-06-24 | 1999-11-30 | Large Scale Biology Corporation | Automated system for two-dimensional electrophoresis |
US5968829A (en) * | 1997-09-05 | 1999-10-19 | Cytotherapeutics, Inc. | Human CNS neural stem cells |
WO1999018438A1 (en) * | 1997-10-02 | 1999-04-15 | Aclara Biosciences, Inc. | Capillary assays involving separation of free and bound species |
US6277259B1 (en) | 1998-04-24 | 2001-08-21 | Enterprise Partners Ii | High performance multidimensional proteome analyzer |
US6013165A (en) | 1998-05-22 | 2000-01-11 | Lynx Therapeutics, Inc. | Electrophoresis apparatus and method |
US6406921B1 (en) | 1998-07-14 | 2002-06-18 | Zyomyx, Incorporated | Protein arrays for high-throughput screening |
US6576478B1 (en) | 1998-07-14 | 2003-06-10 | Zyomyx, Inc. | Microdevices for high-throughput screening of biomolecules |
DE69800630T2 (de) | 1998-07-29 | 2001-08-23 | Agilent Technologies Inc | Chip zur elektroforetischen Trennung von Molekülen und Verfahren zur Verwendung desselben |
SE9803224D0 (sv) * | 1998-09-23 | 1998-09-23 | Amersham Pharm Biotech Ab | Method for separation of macromolecules |
US6240790B1 (en) | 1998-11-09 | 2001-06-05 | Agilent Technologies, Inc. | Device for high throughout sample processing, analysis and collection, and methods of use thereof |
US6156182A (en) | 1998-11-19 | 2000-12-05 | Bio-Rad Laboratories, Inc. | Encapsulated IPG Strips |
EP1044716A1 (de) | 1999-03-13 | 2000-10-18 | Michael Dr. Cahill | Mikropräparative isoelektrischen Fokussierung |
US6638408B1 (en) * | 2000-04-03 | 2003-10-28 | The Wistar Institute | Method and device for separation of charged molecules by solution isoelectric focusing |
US6404905B1 (en) * | 2000-08-31 | 2002-06-11 | Large Scale Proteomics Corp. | Method and apparatus for impressing a master pattern to a gel image |
IL159810A0 (en) * | 2001-07-16 | 2004-06-20 | Protein Forest Inc | Matrixes, arrays, systems and methods |
-
2002
- 2002-07-16 IL IL15981002A patent/IL159810A0/xx unknown
- 2002-07-16 US US10/198,071 patent/US7166202B2/en not_active Expired - Fee Related
- 2002-07-16 AT AT02756507T patent/ATE373236T1/de not_active IP Right Cessation
- 2002-07-16 JP JP2003514267A patent/JP4108037B2/ja not_active Expired - Fee Related
- 2002-07-16 BR BRPI0211213-2A patent/BR0211213A/pt not_active IP Right Cessation
- 2002-07-16 CA CA002454045A patent/CA2454045A1/en not_active Abandoned
- 2002-07-16 CN CNA028169719A patent/CN1549924A/zh active Pending
- 2002-07-16 WO PCT/US2002/022714 patent/WO2003008977A2/en active IP Right Grant
- 2002-07-16 EP EP02756507A patent/EP1410029B1/de not_active Expired - Lifetime
- 2002-07-16 DE DE60222427T patent/DE60222427T2/de not_active Expired - Lifetime
- 2002-07-16 DK DK02756507T patent/DK1410029T3/da active
- 2002-07-16 AU AU2002322513A patent/AU2002322513B2/en not_active Ceased
-
2007
- 2007-01-04 US US11/619,796 patent/US7914656B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
EP1410029A2 (de) | 2004-04-21 |
WO2003008977A3 (en) | 2003-06-19 |
US7914656B2 (en) | 2011-03-29 |
WO2003008977A2 (en) | 2003-01-30 |
CN1549924A (zh) | 2004-11-24 |
EP1410029B1 (de) | 2007-09-12 |
US20030102215A1 (en) | 2003-06-05 |
US7166202B2 (en) | 2007-01-23 |
CA2454045A1 (en) | 2003-01-30 |
US20070138015A1 (en) | 2007-06-21 |
JP4108037B2 (ja) | 2008-06-25 |
DK1410029T3 (da) | 2008-01-07 |
DE60222427T2 (de) | 2008-06-12 |
IL159810A0 (en) | 2004-06-20 |
AU2002322513B2 (en) | 2008-02-28 |
JP2005526949A (ja) | 2005-09-08 |
BR0211213A (pt) | 2006-05-23 |
ATE373236T1 (de) | 2007-09-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |