DE60234464D1 - Zusammensetzungen und Verfahren zur selektiven Nukleinsäureisolierung - Google Patents

Zusammensetzungen und Verfahren zur selektiven Nukleinsäureisolierung

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Publication number
DE60234464D1
DE60234464D1 DE60234464T DE60234464T DE60234464D1 DE 60234464 D1 DE60234464 D1 DE 60234464D1 DE 60234464 T DE60234464 T DE 60234464T DE 60234464 T DE60234464 T DE 60234464T DE 60234464 D1 DE60234464 D1 DE 60234464D1
Authority
DE
Germany
Prior art keywords
methods
compositions
nucleic acid
acid isolation
selective nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60234464T
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English (en)
Inventor
Douglas A Bost
Lawrence I Greenfield
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Applied Biosystems LLC
Original Assignee
Applied Biosystems LLC
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Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23305266&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE60234464(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Applied Biosystems LLC filed Critical Applied Biosystems LLC
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Publication of DE60234464D1 publication Critical patent/DE60234464D1/de
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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
DE60234464T 2001-11-28 2002-11-27 Zusammensetzungen und Verfahren zur selektiven Nukleinsäureisolierung Expired - Lifetime DE60234464D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33402901P 2001-11-28 2001-11-28
PCT/US2002/038123 WO2003046146A2 (en) 2001-11-28 2002-11-27 Compositions and methods of selective nucleic acid isolation

Publications (1)

Publication Number Publication Date
DE60234464D1 true DE60234464D1 (de) 2009-12-31

Family

ID=23305266

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60234464T Expired - Lifetime DE60234464D1 (de) 2001-11-28 2002-11-27 Zusammensetzungen und Verfahren zur selektiven Nukleinsäureisolierung

Country Status (7)

Country Link
US (5) US7208271B2 (de)
EP (1) EP1448799B2 (de)
JP (3) JP4106026B2 (de)
AT (1) ATE449186T1 (de)
AU (1) AU2002359522A1 (de)
DE (1) DE60234464D1 (de)
WO (1) WO2003046146A2 (de)

Families Citing this family (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7078224B1 (en) * 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
DE60234464D1 (de) * 2001-11-28 2009-12-31 Applied Biosystems Llc Zusammensetzungen und Verfahren zur selektiven Nukleinsäureisolierung
JP3644641B2 (ja) * 2003-03-07 2005-05-11 株式会社日立ハイテクノロジーズ 核酸回収方法
JP4241183B2 (ja) * 2003-05-19 2009-03-18 株式会社日立ハイテクノロジーズ 核酸回収方法及び核酸回収キット
US20050009036A1 (en) * 2003-07-11 2005-01-13 Applera Corporation Methods and kits for obtaining nucleic acid from biological samples
CN1882688B (zh) * 2003-07-25 2014-07-16 安比恩股份有限公司 用于分离小rna分子的方法和组合物
US20050059024A1 (en) 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
WO2005012487A2 (en) * 2003-08-01 2005-02-10 Invitrogen Corporation Compositions and methods for preparing short rna molecules and other nucleic acids
JP2005270049A (ja) * 2004-03-26 2005-10-06 Jfe Steel Kk 核酸分子の切断方法
JP2005295869A (ja) * 2004-04-09 2005-10-27 Jfe Steel Kk 核酸分子の切断方法およびその装置
KR100714988B1 (ko) * 2005-08-09 2007-05-09 삼성전자주식회사 산화막이 표면에 형성된 실리콘 구조물 표면을 이용한dna 정제 방법 및 정제장치
EP1963526A4 (de) * 2005-12-09 2009-11-18 Promega Corp Nukleinsäurereinigung mittels bindungsmatrix
WO2007133495A1 (en) * 2006-05-10 2007-11-22 Human Genome Sciences, Inc. Mixture for decreasing protein interference
US20100216657A1 (en) * 2006-05-16 2010-08-26 Arcxis Biotechnologies, Inc. Pcr-free sample preparation and detection systems for high speed biologic analysis and identification
EP3260556B1 (de) 2006-05-31 2019-07-31 Sequenom, Inc. Verfahren zur nukleinsäureextraktion aus einer probe
DE102006031764B4 (de) * 2006-07-06 2009-10-01 Aj Innuscreen Gmbh Verfahren zur parallelen Isolierung doppel- und einzelsträngiger Nukleinsäuren sowie zur selektiven Entfernung doppelsträngiger Nukleinsäuren aus einem Gemisch von doppel- und einzelsträngigen Nukleinsäuren
JP4297148B2 (ja) * 2006-09-22 2009-07-15 ソニー株式会社 核酸回収装置及び核酸回収方法
GB0719022D0 (en) * 2007-09-28 2007-11-07 Mole Genetics As Isolation method
US7871764B1 (en) * 2008-03-18 2011-01-18 The United States Of America As Represented By The United States Department Of Energy Universal nucleic acids sample preparation method for cells, spores and their mixture
WO2010115016A2 (en) 2009-04-03 2010-10-07 Sequenom, Inc. Nucleic acid preparation compositions and methods
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US8222397B2 (en) * 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
EP2588609B1 (de) * 2010-06-29 2018-01-17 Exscale Biospecimen Solutions AB Verfahren und kit zur sequenziellen isolation einer nukleotidspezies aus einer probe
US9024008B2 (en) 2010-07-07 2015-05-05 Diagon Ltd. Procedure for the specific isolation of total DNA content of bacterial germs and a kit for this purpose
DK2742152T3 (en) * 2011-08-12 2017-07-31 Qiagen Gmbh PROCEDURE FOR ISOLATING NUCLEIC ACIDS
FR2994184B1 (fr) 2012-08-02 2017-12-08 Biomerieux Sa Procedes de fonctionalisation et reactifs utilises dans de tels procedes utilisant un anhydride aza-isatoique ou un de ses derives, molecules biologiques ainsi traitees et kits
US20140322706A1 (en) 2012-10-24 2014-10-30 Jon Faiz Kayyem Integrated multipelx target analysis
EP2912432B1 (de) 2012-10-24 2018-07-04 Genmark Diagnostics Inc. Integrierte multiplex-zielanalyse
EP3520895A1 (de) 2013-03-15 2019-08-07 Genmark Diagnostics Inc. Flüssigkeitsbehälter mit freitragender lanze
US9498778B2 (en) 2014-11-11 2016-11-22 Genmark Diagnostics, Inc. Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system
USD881409S1 (en) 2013-10-24 2020-04-14 Genmark Diagnostics, Inc. Biochip cartridge
CN107112105A (zh) 2014-10-23 2017-08-29 康宁股份有限公司 聚合物包封的磁性纳米颗粒
US9598722B2 (en) 2014-11-11 2017-03-21 Genmark Diagnostics, Inc. Cartridge for performing assays in a closed sample preparation and reaction system
US10005080B2 (en) 2014-11-11 2018-06-26 Genmark Diagnostics, Inc. Instrument and cartridge for performing assays in a closed sample preparation and reaction system employing electrowetting fluid manipulation
CN107257857B (zh) 2014-11-14 2021-12-14 康宁股份有限公司 用于ivt后rna纯化的方法和试剂盒
WO2017040992A1 (en) 2015-09-04 2017-03-09 Mo Bio Laboratories, Inc. Methods for co-isolation of nucelic acids and proteins
CN110325650A (zh) 2016-12-22 2019-10-11 夸登特健康公司 用于分析核酸分子的方法和系统
AU2018252018A1 (en) 2017-04-14 2019-10-10 Guardant Health, Inc. Methods of attaching adapters to sample nucleic acids
EP3685386A1 (de) 2017-09-20 2020-07-29 Guardant Health, Inc. Verfahren und systeme zur differenzierung von somatischen und keimbahnvarianten
WO2019090156A1 (en) 2017-11-03 2019-05-09 Guardant Health, Inc. Normalizing tumor mutation burden
WO2019090147A1 (en) 2017-11-03 2019-05-09 Guardant Health, Inc. Correcting for deamination-induced sequence errors
GB201718803D0 (en) * 2017-11-14 2017-12-27 Phoenix Molecular Pte Ltd Method for isolation of nucleic acids
CN108456675A (zh) * 2018-03-27 2018-08-28 爱默基因(厦门)生物科技有限公司 一种用于凝胶DNA回收的溶胶buffer、回收凝胶DNA的试剂盒及其使用方法
EP3802878A1 (de) 2018-06-04 2021-04-14 Guardant Health, Inc. Verfahren und systeme zur bestimmung des zellulären ursprungs zellfreier nukleinsäuren
WO2020023420A2 (en) 2018-07-23 2020-01-30 Guardant Health, Inc. Methods and systems for adjusting tumor mutational burden by tumor fraction and coverage
CA3109646A1 (en) 2018-08-30 2020-03-05 Guardant Health, Inc. Methods and systems for detecting contamination between samples
JP2021536612A (ja) 2018-08-31 2021-12-27 ガーダント ヘルス, インコーポレイテッド マージされたリードおよびマージされないリードに基づいた遺伝的変異体の検出
CN112930569A (zh) 2018-08-31 2021-06-08 夸登特健康公司 无细胞dna中的微卫星不稳定性检测
WO2020096691A2 (en) 2018-09-04 2020-05-14 Guardant Health, Inc. Methods and systems for detecting allelic imbalance in cell-free nucleic acid samples
US20200131566A1 (en) 2018-10-31 2020-04-30 Guardant Health, Inc. Methods, compositions and systems for calibrating epigenetic partitioning assays
JP2022514010A (ja) 2018-12-20 2022-02-09 ガーダント ヘルス, インコーポレイテッド 核酸分子の回収率を改善するための方法、組成物、およびシステム
JP2022519045A (ja) 2019-01-31 2022-03-18 ガーダント ヘルス, インコーポレイテッド 無細胞dnaを単離するための組成物および方法
AU2020228058A1 (en) 2019-02-27 2021-09-23 Guardant Health, Inc. Computational modeling of loss of function based on allelic frequency
WO2020176659A1 (en) 2019-02-27 2020-09-03 Guardant Health, Inc. Methods and systems for determining the cellular origin of cell-free dna
US11939636B2 (en) 2019-05-31 2024-03-26 Guardant Health, Inc. Methods and systems for improving patient monitoring after surgery
CA3151538A1 (en) 2019-09-30 2021-04-08 Stefanie Ann Ward Mortimer Compositions and methods for analyzing cell-free dna in methylation partitioning assays
US11447819B2 (en) 2019-10-25 2022-09-20 Guardant Health, Inc. Methods for 3′ overhang repair
CN114746560A (zh) 2019-11-26 2022-07-12 夸登特健康公司 改进甲基化多核苷酸结合的方法、组合物和系统
EP4097724A1 (de) 2020-01-31 2022-12-07 Guardant Health, Inc. Signifikanzmodellierung der abwesenheit von zielvarianten auf klonaler ebene
JP2023517029A (ja) 2020-03-11 2023-04-21 ガーダント ヘルス, インコーポレイテッド 無細胞核酸において検出された遺伝的突然変異を、腫瘍起源または非腫瘍起源として分類するための方法
EP4143338A1 (de) 2020-04-30 2023-03-08 Guardant Health, Inc. Verfahren zur sequenzbestimmung unter verwendung partitionierter nukleinsäuren
US20220025468A1 (en) 2020-05-14 2022-01-27 Guardant Health, Inc. Homologous recombination repair deficiency detection
WO2023282916A1 (en) 2021-07-09 2023-01-12 Guardant Health, Inc. Methods of detecting genomic rearrangements using cell free nucleic acids
EP4189111A1 (de) 2020-07-30 2023-06-07 Guardant Health, Inc. Verfahren zur isolierung von zellfreier dna
EP4205126A1 (de) 2020-08-25 2023-07-05 Guardant Health, Inc. Verfahren und systeme zur vorhersage des ursprungs einer variante
EP4205122A2 (de) 2020-08-27 2023-07-05 Guardant Health, Inc. Rechnerische detektion von kopienzahlvariationen an einem locus in abwesenheit von direkter messung des locus
WO2022073011A1 (en) 2020-09-30 2022-04-07 Guardant Health, Inc. Methods and systems to improve the signal to noise ratio of dna methylation partitioning assays
US20220154286A1 (en) 2020-10-23 2022-05-19 Guardant Health, Inc. Compositions and methods for analyzing dna using partitioning and base conversion
JP2023551292A (ja) 2020-11-30 2023-12-07 ガーダント ヘルス, インコーポレイテッド メチル化されたポリヌクレオチドを富化するための組成物および方法
WO2022140629A1 (en) 2020-12-23 2022-06-30 Guardant Health, Inc. Methods and systems for analyzing methylated polynucleotides
WO2022174109A1 (en) 2021-02-12 2022-08-18 Guardant Health, Inc. Methods and compositions for detecting nucleic acid variants
CA3210101A1 (en) 2021-03-05 2022-09-09 Katie Julia QUINN Methods and related aspects for analyzing molecular response
JP2024512372A (ja) 2021-03-09 2024-03-19 ガーダント ヘルス, インコーポレイテッド オフターゲットポリヌクレオチド配列決定データに基づく腫瘍の存在の検出
WO2022204730A1 (en) 2021-03-25 2022-09-29 Guardant Health, Inc. Methods and compositions for quantifying immune cell dna
EP4348249A1 (de) 2021-05-28 2024-04-10 Guardant Health, Inc. Zusammensetzungen und verfahren zur untersuchung von zirkulierenden molekülen
WO2022271730A1 (en) 2021-06-21 2022-12-29 Guardant Health, Inc. Methods and compositions for copy-number informed tissue-of-origin analysis
WO2023023402A2 (en) 2021-08-20 2023-02-23 Guardant Health, Inc. Methods for simultaneous molecular and sample barcoding
WO2023056065A1 (en) 2021-09-30 2023-04-06 Guardant Health, Inc. Compositions and methods for synthesis and use of probes targeting nucleic acid rearrangements
WO2023081722A2 (en) 2021-11-02 2023-05-11 Guardant Health, Inc. Quality control method
WO2023122623A1 (en) 2021-12-21 2023-06-29 Guardant Health, Inc. Methods and systems for combinatorial chromatin-ip sequencing
WO2023122740A1 (en) 2021-12-23 2023-06-29 Guardant Health, Inc. Compositions and methods for detection of metastasis
WO2023150633A2 (en) 2022-02-02 2023-08-10 Guardant Health, Inc. Multifunctional primers for paired sequencing reads
WO2023197004A1 (en) 2022-04-07 2023-10-12 Guardant Health, Inc. Detecting the presence of a tumor based on methylation status of cell-free nucleic acid molecules
US20230360725A1 (en) 2022-05-09 2023-11-09 Guardant Health, Inc. Detecting degradation based on strand bias
WO2024006908A1 (en) 2022-06-30 2024-01-04 Guardant Health, Inc. Enrichment of aberrantly methylated dna
WO2024020573A1 (en) 2022-07-21 2024-01-25 Guardant Health, Inc. Methods for detection and reduction of sample preparation-induced methylation artifacts
WO2024059840A1 (en) 2022-09-16 2024-03-21 Guardant Health, Inc. Compositions and methods for analyzing soluble proteins
WO2024073508A2 (en) 2022-09-27 2024-04-04 Guardant Health, Inc. Methods and compositions for quantifying immune cell dna

Family Cites Families (68)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4340674A (en) * 1980-05-05 1982-07-20 The Upjohn Company Cointegrate plasmids and their construction from plasmids of Escherichia and Streptomyces
US4420517A (en) 1982-05-06 1983-12-13 Becton Dickinson And Company Methods for improving uniformity of silica films on substrates
US4483920A (en) 1982-05-17 1984-11-20 Hahnemann University Immobilization of message RNA directly from cells onto filter material
DE3308932A1 (de) 1983-03-12 1984-09-13 Hoechst Ag, 6230 Frankfurt Verfahren zur abtrennung von ribonukleinsaeuren aus einer loesung, die desoxyribonukleinsaeuren enthaelt
US5137816A (en) * 1983-06-22 1992-08-11 The Lubrizol Corporation Rhizobial diagnostic probes and rhizobium trifolii nifH promoters
US4648975A (en) 1983-08-17 1987-03-10 Pedro B. Macedo Process of using improved silica-based chromatographic supports containing additives
GB8519457D0 (en) * 1985-08-02 1985-09-11 Faulk Ward Page Tumour imaging agents
CA1301606C (en) 1986-05-02 1992-05-26 David H. Gillespie Chaotropic method for evaluating nucleic acids in a biological sample
US4900677A (en) * 1986-09-26 1990-02-13 E. I. Du Pont De Nemours And Company Process for rapid isolation of high molecular weight DNA
US4843155A (en) 1987-11-19 1989-06-27 Piotr Chomczynski Product and process for isolating RNA
US4923978A (en) 1987-12-28 1990-05-08 E. I. Du Pont De Nemours & Company Process for purifying nucleic acids
US5075430A (en) * 1988-12-12 1991-12-24 Bio-Rad Laboratories, Inc. Process for the purification of DNA on diatomaceous earth
NL8900725A (nl) 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
CA2011929A1 (en) 1989-04-03 1990-10-03 Rex M. Bitner Metal oxide supports for nucleic acids
US5106966A (en) 1989-07-10 1992-04-21 University Of Kansas Use of gel polymer for dna extraction with organic solvents
US5175271A (en) 1989-07-10 1992-12-29 University Of Kansas Use of silica gel for DNA extraction with organic solvents
US5533888A (en) * 1990-08-21 1996-07-09 Belarde; John F. Apparatus for forming concrete barriers
WO1992018514A1 (en) 1991-04-12 1992-10-29 Minnesota Mining And Manufacturing Company Purification of nucleic acids using metal oxide supports
CA2067711C (en) 1991-05-03 2000-08-08 Daniel Lee Woodard Solid phase extraction purification of dna
US5155018A (en) * 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
US5329000A (en) 1991-10-31 1994-07-12 Becton, Dickinson And Company Purification of DNA with silicon tetrahydrazide
DE4143639C2 (de) 1991-12-02 2002-10-24 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren
US5346994A (en) 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
DE69324716T2 (de) 1992-02-13 1999-09-09 Becton Dickinson Co Celithydrat und Reinigung von DNS
CA2102264C (en) 1992-11-13 2000-08-01 Daniel Lee Woodard Boron silicates, aluminum silicates, phosphosilicates and purification of dna
DE4321904B4 (de) 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
WO1995004140A1 (en) 1993-07-28 1995-02-09 Akzo Nobel N.V. Process for isolating nucleic acid from gram positive microorganisms
WO1995006652A1 (en) 1993-08-30 1995-03-09 Promega Corporation Nucleic acid purification compositions and methods
US5637687A (en) 1993-08-31 1997-06-10 Wiggins; James C. Methods and compositions for isolating nucleic acids
US5503816A (en) 1993-09-27 1996-04-02 Becton Dickinson And Company Silicate compounds for DNA purification
US5438129A (en) 1993-09-27 1995-08-01 Becton Dickinson And Company DNA purification by solid phase extraction using partially fluorinated aluminum hydroxide adsorbant
US5438127A (en) 1993-09-27 1995-08-01 Becton Dickinson And Company DNA purification by solid phase extraction using a PCl3 modified glass fiber membrane
ATE302208T1 (de) 1994-02-11 2005-09-15 Qiagen Gmbh Verfahren zur trennung von doppelstrang/einzelstrangnukleinsäurestrukturen
US5705628A (en) * 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5641665A (en) * 1994-11-28 1997-06-24 Vical Incorporated Plasmids suitable for IL-2 expression
GB9425138D0 (en) * 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US5576196A (en) 1995-01-13 1996-11-19 Vical Incorporated Process for reducing RNA concentration in a mixture of biological material using diatomaceous earth
US5777099A (en) 1995-02-24 1998-07-07 Biotecx Laboratories, Inc. RNA separation
JP2965131B2 (ja) 1995-07-07 1999-10-18 東洋紡績株式会社 核酸結合用磁性担体およびそれを用いる核酸単離方法
US5945515A (en) 1995-07-31 1999-08-31 Chomczynski; Piotr Product and process for isolating DNA, RNA and proteins
US5804684A (en) 1995-08-24 1998-09-08 The Theobald Smith Research Institute, Inc. Method for isolating nucleic acids
US5783686A (en) 1995-09-15 1998-07-21 Beckman Instruments, Inc. Method for purifying nucleic acids from heterogenous mixtures
DE29601618U1 (de) 1996-01-31 1996-07-04 Invitek Gmbh Vorrichtung zur gleichzeitigen multiplen Isolierung
GB9602825D0 (en) 1996-02-12 1996-04-10 Therexsys Ltd Method of plasmid dna production and purification
US5973137A (en) 1996-02-13 1999-10-26 Gentra Systems, Inc. Low pH RNA isolation reagents, method, and kit
KR100483498B1 (ko) 1996-02-14 2005-07-18 악조 노벨 엔.브이. 핵산물질의분리및증폭방법
ZA973642B (en) * 1996-04-26 1997-11-25 Merck & Co Inc DNA vaccine formulations.
EP0814156B1 (de) 1996-06-18 2003-03-05 The Institute Of Physical & Chemical Research Verfahren zur Reinigung von DNS
US5990302A (en) 1996-07-12 1999-11-23 Toyo Boseki Kabushiki Kaisha Method for isolating ribonucleic acid
US5981235A (en) 1996-07-29 1999-11-09 Promega Corporation Methods for isolating nucleic acids using alkaline protease
CA2214495C (en) 1996-09-25 2002-02-05 Daniel L. Woodard Hydrated zirconium silicate composition for purification of nucleic acids
JP2001506005A (ja) 1996-12-11 2001-05-08 ナイコメッド・アマーシャム・ピーエルシー 細胞の選択的溶解
JP2001524820A (ja) 1997-04-10 2001-12-04 ライフ テクノロジーズ,インコーポレイテッド Rna単離試薬および方法
DE69836587T2 (de) * 1997-04-16 2007-10-11 Applera Corp., Foster City Nukleinsäuresammlung
US6218531B1 (en) 1997-06-25 2001-04-17 Promega Corporation Method of isolating RNA
US5972613A (en) 1997-12-09 1999-10-26 The Perkin-Elmer Corporation Methods of nucleic acid isolation
DE19801730A1 (de) 1998-01-19 1999-07-22 Fraunhofer Ges Forschung Verfahren zur kombinierten Analyse von Zellinhalten, insbesondere den in biologischen Zellen enthaltenen Nukleinsäuren sowie Vorrichtung zur Durchführung des Verfahrens
EP1053357B1 (de) 1998-02-02 2008-05-21 QIAGEN North American Holdings, Inc. Verfahren zur isolierung, amplifizierung und charakterisierung von dns
EP1071691B1 (de) 1998-02-04 2005-09-28 MERCK PATENT GmbH Verfahren zur isolierung und aufreinigung von nucleinsäuren
CN1299413A (zh) 1998-04-02 2001-06-13 李璟日 玻璃微纤维柱及用其制备和纯化质粒dna的方法
EP0969090A1 (de) * 1998-05-27 2000-01-05 QIAGEN GmbH Schnelles und einfaches Verfahren zur Isolierung von zirkulären Nucleinsäuren
WO2000063362A1 (en) 1999-04-21 2000-10-26 Annovis, Inc. Magnetic dna extraction kit for plants
US6270970B1 (en) 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
ATE280225T1 (de) 1999-08-20 2004-11-15 Promega Corp Simultane isolierung und quantifizierung von dna
US7001724B1 (en) 2000-11-28 2006-02-21 Applera Corporation Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases
AU2002211719B2 (en) 2001-10-12 2007-06-14 Qiagen North American Holdings, Inc. Compositions and methods for using a solid support to purify RNA
DE60234464D1 (de) 2001-11-28 2009-12-31 Applied Biosystems Llc Zusammensetzungen und Verfahren zur selektiven Nukleinsäureisolierung

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EP1448799B2 (de) 2018-05-16
JP4106026B2 (ja) 2008-06-25
EP1448799A2 (de) 2004-08-25
US20150086988A1 (en) 2015-03-26
JP2008000146A (ja) 2008-01-10
WO2003046146A3 (en) 2004-02-26
AU2002359522A8 (en) 2003-06-10
JP2009039137A (ja) 2009-02-26
JP2005510233A (ja) 2005-04-21
WO2003046146A2 (en) 2003-06-05
US20100021905A1 (en) 2010-01-28
AU2002359522A1 (en) 2003-06-10
ATE449186T1 (de) 2009-12-15
US20030138828A1 (en) 2003-07-24
US7208271B2 (en) 2007-04-24
EP1448799B1 (de) 2009-11-18
US8507198B2 (en) 2013-08-13
EP1448799A4 (de) 2006-03-08
US20070224630A1 (en) 2007-09-27
US7537898B2 (en) 2009-05-26
US20140065626A1 (en) 2014-03-06

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