DE60239386D1 - Zellbibliothek - Google Patents
ZellbibliothekInfo
- Publication number
- DE60239386D1 DE60239386D1 DE60239386T DE60239386T DE60239386D1 DE 60239386 D1 DE60239386 D1 DE 60239386D1 DE 60239386 T DE60239386 T DE 60239386T DE 60239386 T DE60239386 T DE 60239386T DE 60239386 D1 DE60239386 D1 DE 60239386D1
- Authority
- DE
- Germany
- Prior art keywords
- novel
- libraries
- host cells
- cell library
- discovery
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DKPA200100128 | 2001-01-25 | ||
DKPA200100679 | 2001-05-01 | ||
US30086301P | 2001-06-27 | 2001-06-27 | |
PCT/DK2002/000056 WO2002059297A2 (en) | 2001-01-25 | 2002-01-25 | A library of a collection of cells |
Publications (1)
Publication Number | Publication Date |
---|---|
DE60239386D1 true DE60239386D1 (de) | 2011-04-21 |
Family
ID=43646216
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60239386T Expired - Lifetime DE60239386D1 (de) | 2001-01-25 | 2002-01-25 | Zellbibliothek |
Country Status (9)
Country | Link |
---|---|
US (2) | US7838287B2 (de) |
EP (1) | EP1356037B1 (de) |
JP (2) | JP2004525624A (de) |
AT (1) | ATE501251T1 (de) |
AU (1) | AU2002227883A1 (de) |
CA (1) | CA2474172C (de) |
DE (1) | DE60239386D1 (de) |
DK (1) | DK1356037T3 (de) |
WO (1) | WO2002059297A2 (de) |
Families Citing this family (43)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8008459B2 (en) | 2001-01-25 | 2011-08-30 | Evolva Sa | Concatemers of differentially expressed multiple genes |
ATE501251T1 (de) | 2001-01-25 | 2011-03-15 | Evolva Ltd | Zellbibliothek |
AU2003237662B2 (en) * | 2002-01-25 | 2008-04-24 | Evolva Ltd | Methods for multiple parameter screening and evolution of cells to produce small molecules with multiple functionalities |
USRE47770E1 (en) | 2002-07-18 | 2019-12-17 | Merus N.V. | Recombinant production of mixtures of antibodies |
ES2368733T3 (es) | 2002-07-18 | 2011-11-21 | Merus B.V. | Producción recombinante de mezclas de anticuerpos. |
DK1529110T3 (da) * | 2002-08-01 | 2012-01-30 | Evolva Ltd | Fremgangsmåde til blanding af store antal af heterologe gener |
EP1639009B1 (de) | 2003-05-30 | 2013-02-27 | Merus B.V. | Fab-bibliothek zur herstellung von einer mischung von antikörpern |
US20100069614A1 (en) | 2008-06-27 | 2010-03-18 | Merus B.V. | Antibody producing non-human mammals |
JP5912211B2 (ja) * | 2004-01-20 | 2016-04-27 | メルス ビー.ヴィー. | 結合タンパク質の混合物 |
AU2006227165B2 (en) | 2005-03-18 | 2011-11-10 | Microbia, Inc. | Production of carotenoids in oleaginous yeast and fungi |
US8691555B2 (en) | 2006-09-28 | 2014-04-08 | Dsm Ip Assests B.V. | Production of carotenoids in oleaginous yeast and fungi |
EP2370451B1 (de) | 2008-12-02 | 2016-11-16 | Wave Life Sciences Japan, Inc. | Verfahren zur synthese von am phosphoratom modifizierten nukleinsäuren |
KR101153866B1 (ko) | 2009-05-21 | 2012-06-27 | 대한민국 | 형질전환용 운반체, 이의 제조방법, 이를 이용한 형질전환방법 및 형질전환체 |
SG177564A1 (en) | 2009-07-06 | 2012-02-28 | Ontorii Inc | Novel nucleic acid prodrugs and methods of use thereof |
US9476082B2 (en) | 2010-05-20 | 2016-10-25 | Evolva Sa | Method of producing isoprenoid compounds in yeast |
BR122021005287B1 (pt) | 2010-06-02 | 2022-02-22 | Evolva, Inc | Hospedeiro recombinante que compreende um gene recombinante para produção de esteviol ou glicosídeo de esteviol |
JP5868324B2 (ja) | 2010-09-24 | 2016-02-24 | 株式会社Wave Life Sciences Japan | 不斉補助基 |
RU2014105311A (ru) | 2011-07-19 | 2015-08-27 | Уэйв Лайф Сайенсес Пте. Лтд. | Способы синтеза функционализованных нуклеиновых кислот |
SG10201606567TA (en) | 2011-08-08 | 2016-10-28 | Evolva Sa | Recombinant production of steviol glycosides |
EA202090591A3 (ru) | 2012-04-20 | 2020-12-30 | Мерюс Н.В. | СПОСОБЫ И СРЕДСТВА ДЛЯ ПОЛУЧЕНИЯ Ig-ПОДОБНЫХ МОЛЕКУЛ |
US10829772B2 (en) | 2012-06-22 | 2020-11-10 | Monsanto Technology Llc | Unique modular vector design |
SG11201500239VA (en) | 2012-07-13 | 2015-03-30 | Wave Life Sciences Japan | Asymmetric auxiliary group |
SG11201500243WA (en) | 2012-07-13 | 2015-04-29 | Shin Nippon Biomedical Lab Ltd | Chiral nucleic acid adjuvant |
US9982257B2 (en) | 2012-07-13 | 2018-05-29 | Wave Life Sciences Ltd. | Chiral control |
US20150293076A1 (en) * | 2012-10-22 | 2015-10-15 | Bolt Threads, Inc. | Cellular Reprogramming for Product Optimization |
KR20150115002A (ko) | 2013-02-06 | 2015-10-13 | 에볼바 에스아 | 레바우디오시드 d 및 레바우디오시드 m의 개선된 생산 방법 |
BR112015019160A2 (pt) | 2013-02-11 | 2017-08-22 | Dalgaard Mikkelsen Michael | Produção de glicosídeos de esteviol em hospedeiros recombinantes |
JP6556122B2 (ja) | 2013-09-17 | 2019-08-07 | ボルト スレッズ インコーポレイテッド | 改良シルク繊維を合成するための方法および組成物 |
US9580758B2 (en) | 2013-11-12 | 2017-02-28 | Luc Montagnier | System and method for the detection and treatment of infection by a microbial agent associated with HIV infection |
JPWO2015108046A1 (ja) | 2014-01-15 | 2017-03-23 | 株式会社新日本科学 | 抗アレルギー作用を有するキラル核酸アジュバンド及び抗アレルギー剤 |
JPWO2015108047A1 (ja) | 2014-01-15 | 2017-03-23 | 株式会社新日本科学 | 免疫誘導活性を有するキラル核酸アジュバンド及び免疫誘導活性剤 |
US10149905B2 (en) | 2014-01-15 | 2018-12-11 | Shin Nippon Biomedical Laboratories, Ltd. | Chiral nucleic acid adjuvant having antitumor effect and antitumor agent |
DK3094728T3 (da) | 2014-01-16 | 2022-05-16 | Wave Life Sciences Ltd | Kiralt design |
SG11201700651RA (en) | 2014-08-11 | 2017-02-27 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
WO2016038095A2 (en) | 2014-09-09 | 2016-03-17 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
WO2016120486A1 (en) | 2015-01-30 | 2016-08-04 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
CN107567492B (zh) | 2015-03-16 | 2022-10-14 | 帝斯曼知识产权资产管理有限公司 | Udp-糖基转移酶 |
AU2016307066A1 (en) | 2015-08-07 | 2018-02-08 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
JP2019513392A (ja) | 2016-04-13 | 2019-05-30 | エヴォルヴァ エスアー.Evolva Sa. | 組み換え宿主におけるステビオールグリコシドの産生 |
US10815514B2 (en) | 2016-05-16 | 2020-10-27 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
WO2018053204A1 (en) | 2016-09-14 | 2018-03-22 | Bolt Threads, Inc. | Long uniform recombinant protein fibers |
US11396669B2 (en) | 2016-11-07 | 2022-07-26 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
JP7212982B1 (ja) * | 2022-10-05 | 2023-01-26 | 株式会社Logomix | 細胞ライブラリおよびその製造方法 |
Family Cites Families (53)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1341116C (en) | 1983-02-22 | 2000-10-17 | Rae Lyn Burke | Yeast expression systems with vectors having gapdh or pyk promoters and synthesis or foreign protein |
ATE116442T1 (de) | 1988-04-22 | 1995-01-15 | Massachusetts Inst Technology | Verfahren zum formen und zum gebrauch von mikrotröpchen. |
ATE160176T1 (de) | 1989-07-07 | 1997-11-15 | Unilever Nv | Verfahren zur herstellung eines proteins mittels eines durch mehrfachkopieintegrierung eines expressionsvektors transformierten pilzes |
US5316922A (en) * | 1992-04-15 | 1994-05-31 | The United States Of America As Represented By Department Of Health And Human Services | Method for indentifying and expressing proteins that recognize and adhere to specific probes |
US5482845A (en) | 1993-09-24 | 1996-01-09 | The Trustees Of Columbia University In The City Of New York | Method for construction of normalized cDNA libraries |
EP0730659A4 (de) | 1993-10-27 | 1999-03-31 | Univ Columbia | Methode zur Bildung einersubtrahierten cDNA Bank und ihre Verwendung |
US6268140B1 (en) * | 1994-02-03 | 2001-07-31 | Neugenesis | Combinatorial metabolic libraries |
US6309883B1 (en) * | 1994-02-17 | 2001-10-30 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
US5837458A (en) * | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
US6117679A (en) * | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US6165793A (en) * | 1996-03-25 | 2000-12-26 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
AU723619B2 (en) * | 1995-04-24 | 2000-08-31 | Chromaxome Corporation | Methods for generating and screening novel metabolic pathways |
US6030779A (en) * | 1995-07-18 | 2000-02-29 | Diversa Corporation | Screening for novel bioactivities |
US6057103A (en) * | 1995-07-18 | 2000-05-02 | Diversa Corporation | Screening for novel bioactivities |
US5958672A (en) * | 1995-07-18 | 1999-09-28 | Diversa Corporation | Protein activity screening of clones having DNA from uncultivated microorganisms |
US6004788A (en) * | 1995-07-18 | 1999-12-21 | Diversa Corporation | Enzyme kits and libraries |
US20030215798A1 (en) | 1997-06-16 | 2003-11-20 | Diversa Corporation | High throughput fluorescence-based screening for novel enzymes |
US6537776B1 (en) | 1999-06-14 | 2003-03-25 | Diversa Corporation | Synthetic ligation reassembly in directed evolution |
US5939250A (en) * | 1995-12-07 | 1999-08-17 | Diversa Corporation | Production of enzymes having desired activities by mutagenesis |
US20010041333A1 (en) * | 1997-06-16 | 2001-11-15 | Short Jay M. | High throughput screening for a bioactivity or biomolecule |
GB9603803D0 (en) * | 1996-02-22 | 1996-04-24 | Zeneca Ltd | Production of proteins, plasmids coding therefor and organisms containing such plasmids |
US6025155A (en) * | 1996-04-10 | 2000-02-15 | Chromos Molecular Systems, Inc. | Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes |
US5783431A (en) * | 1996-04-24 | 1998-07-21 | Chromaxome Corporation | Methods for generating and screening novel metabolic pathways |
US6242211B1 (en) | 1996-04-24 | 2001-06-05 | Terragen Discovery, Inc. | Methods for generating and screening novel metabolic pathways |
WO1997044470A1 (en) | 1996-05-21 | 1997-11-27 | Novo Nordisk A/S | Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast |
US5763239A (en) * | 1996-06-18 | 1998-06-09 | Diversa Corporation | Production and use of normalized DNA libraries |
US6294325B1 (en) * | 1996-07-05 | 2001-09-25 | The Mount Sinai School Of Medicine Of The City University Of New York | Cloning and expression of thermostable multi genes and proteins and uses thereof |
AU6487196A (en) | 1996-07-09 | 1998-02-02 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Transformation-associated recombination cloning |
EP1007732B1 (de) | 1997-01-17 | 2006-07-26 | Maxygen, Inc. | EVOLUTION Prokaryotischer GANZER ZELLEN DURCH REKURSIVE SEQUENZREKOMBINATION |
US6326204B1 (en) | 1997-01-17 | 2001-12-04 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
JP3683591B2 (ja) | 1997-02-27 | 2005-08-17 | セロミックス インコーポレイテッド | 細胞に基づくスクリーニングシステム |
WO1998041869A1 (en) | 1997-03-18 | 1998-09-24 | Chromaxome Corporation | Methods for screening compounds using encapsulated cells |
US6391585B1 (en) | 1997-05-27 | 2002-05-21 | Hanil Synthetic Fiber Co., Ltd. | Process for preparing recombinant proteins using highly efficient expression vector from saccharomyces cerevisiae |
GB9727512D0 (en) | 1997-12-31 | 1998-02-25 | Adprotech Plc | Fuzzy genes and their application in molecular adjuvants |
AU744699B2 (en) | 1998-03-04 | 2002-02-28 | Verenium Corporation | Production and use of normalized DNA libraries |
WO2000006715A1 (en) | 1998-07-27 | 2000-02-10 | Genotypes Inc. | Automatic eukaryotic artificial chromosome vector |
JP2002531056A (ja) | 1998-08-07 | 2002-09-24 | セレイ, エルエルシー | 遺伝子分析におけるゲルマイクロドロップ |
AU5696899A (en) | 1998-08-31 | 2000-03-21 | Maxygen, Inc. | Transformation, selection, and screening of sequence-shuffled polynucleotides for development and optimization of plant phenotypes |
AU6048599A (en) | 1998-09-18 | 2000-04-10 | Cellomics, Inc. | A system for cell-based screening |
IL145165A0 (en) | 1999-03-09 | 2002-06-30 | Diversa Corp | End selection in directed evolution |
CA2329122A1 (en) | 1999-03-26 | 2000-10-05 | Diversa Corporation | Exonuclease-mediated nucleic acid reassembly in directed evolution |
GB9908814D0 (en) * | 1999-04-16 | 1999-06-09 | Celltech Therapeutics Ltd | Process |
AU7646000A (en) | 1999-11-05 | 2001-05-14 | Novozymes A/S | Methods to screen microorganisms or gene libraries for products secreted from a cell |
AU2001287273A1 (en) | 2000-03-24 | 2001-10-08 | Maxygen, Inc. | Methods for modulating cellular and organismal phenotypes |
CA2474146C (en) | 2001-01-25 | 2012-09-18 | Evolva Ltd. | A method for evolving a cell having desired phenotype and evolved cells |
ATE501251T1 (de) | 2001-01-25 | 2011-03-15 | Evolva Ltd | Zellbibliothek |
EP1360308B1 (de) | 2001-01-25 | 2007-10-10 | Evolva Ltd. | Concatemere unterschiedlich exprimierter multipler gene |
US8008459B2 (en) * | 2001-01-25 | 2011-08-30 | Evolva Sa | Concatemers of differentially expressed multiple genes |
AU2002226307A1 (en) | 2001-01-25 | 2002-08-06 | Evolva Biotech A/S | Artificial chromosomes comprising concatemers of expressible nucleotide sequences |
AU2003237662B2 (en) * | 2002-01-25 | 2008-04-24 | Evolva Ltd | Methods for multiple parameter screening and evolution of cells to produce small molecules with multiple functionalities |
EP1558741B1 (de) | 2002-06-14 | 2008-02-20 | Dyax Corporation | Rekombination von nukleinsäurebibliotheksmitgliedern |
US20030235823A1 (en) * | 2002-06-24 | 2003-12-25 | The University Of Alabama | Nucleotide sequences that code for torsin genes, torsin proteins, and methods of using the same to treat protein-aggregation |
DK1529110T3 (da) | 2002-08-01 | 2012-01-30 | Evolva Ltd | Fremgangsmåde til blanding af store antal af heterologe gener |
-
2002
- 2002-01-25 AT AT02709979T patent/ATE501251T1/de not_active IP Right Cessation
- 2002-01-25 WO PCT/DK2002/000056 patent/WO2002059297A2/en active Application Filing
- 2002-01-25 EP EP02709979A patent/EP1356037B1/de not_active Expired - Lifetime
- 2002-01-25 US US10/466,960 patent/US7838287B2/en not_active Expired - Fee Related
- 2002-01-25 DK DK02709979.5T patent/DK1356037T3/da active
- 2002-01-25 CA CA2474172A patent/CA2474172C/en not_active Expired - Fee Related
- 2002-01-25 JP JP2002559584A patent/JP2004525624A/ja not_active Withdrawn
- 2002-01-25 DE DE60239386T patent/DE60239386D1/de not_active Expired - Lifetime
- 2002-01-25 AU AU2002227883A patent/AU2002227883A1/en not_active Abandoned
-
2009
- 2009-10-07 JP JP2009233636A patent/JP4965625B2/ja not_active Expired - Fee Related
-
2010
- 2010-10-12 US US12/902,587 patent/US20120094870A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP1356037B1 (de) | 2011-03-09 |
WO2002059297A3 (en) | 2003-04-10 |
WO2002059297A2 (en) | 2002-08-01 |
WO2002059297A8 (en) | 2004-04-15 |
US7838287B2 (en) | 2010-11-23 |
JP2004525624A (ja) | 2004-08-26 |
CA2474172A1 (en) | 2002-08-01 |
ATE501251T1 (de) | 2011-03-15 |
US20120094870A1 (en) | 2012-04-19 |
AU2002227883A1 (en) | 2002-08-06 |
EP1356037A2 (de) | 2003-10-29 |
DK1356037T3 (da) | 2011-06-27 |
US20040241672A1 (en) | 2004-12-02 |
JP4965625B2 (ja) | 2012-07-04 |
JP2010043108A (ja) | 2010-02-25 |
CA2474172C (en) | 2012-07-10 |
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Legal Events
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