DE60332873D1 - Rolling-circle rna-amplifikation - Google Patents
Rolling-circle rna-amplifikationInfo
- Publication number
- DE60332873D1 DE60332873D1 DE60332873T DE60332873T DE60332873D1 DE 60332873 D1 DE60332873 D1 DE 60332873D1 DE 60332873 T DE60332873 T DE 60332873T DE 60332873 T DE60332873 T DE 60332873T DE 60332873 D1 DE60332873 D1 DE 60332873D1
- Authority
- DE
- Germany
- Prior art keywords
- molecules
- rna molecules
- sequence
- strand cdna
- cdna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000003321 amplification Effects 0.000 title abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 2
- 239000002299 complementary DNA Substances 0.000 abstract 7
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 6
- 238000000034 method Methods 0.000 abstract 4
- 239000000523 sample Substances 0.000 abstract 3
- 230000010076 replication Effects 0.000 abstract 2
- 238000005096 rolling process Methods 0.000 abstract 2
- 238000010804 cDNA synthesis Methods 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 108020004999 messenger RNA Proteins 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 230000003362 replicative effect Effects 0.000 abstract 1
- 230000002194 synthesizing effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/335,573 US6977153B2 (en) | 2002-12-31 | 2002-12-31 | Rolling circle amplification of RNA |
| PCT/US2003/039430 WO2004061119A2 (en) | 2002-12-31 | 2003-12-11 | Rolling circle amplification of rna |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE60332873D1 true DE60332873D1 (de) | 2010-07-15 |
Family
ID=32655391
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60332873T Expired - Lifetime DE60332873D1 (de) | 2002-12-31 | 2003-12-11 | Rolling-circle rna-amplifikation |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US6977153B2 (de) |
| EP (1) | EP1585833B1 (de) |
| JP (1) | JP2006512081A (de) |
| AT (1) | ATE469983T1 (de) |
| AU (1) | AU2003297891A1 (de) |
| CA (1) | CA2512196A1 (de) |
| DE (1) | DE60332873D1 (de) |
| WO (1) | WO2004061119A2 (de) |
Families Citing this family (72)
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| US9487823B2 (en) | 2002-12-20 | 2016-11-08 | Qiagen Gmbh | Nucleic acid amplification |
| US7955795B2 (en) | 2003-06-06 | 2011-06-07 | Qiagen Gmbh | Method of whole genome amplification with reduced artifact production |
| US7309571B2 (en) * | 2003-02-03 | 2007-12-18 | Ge Healthcare Bio-Sciences Corp. | Amplification of self-ligated, circularized cDNA for expression profiling |
| US8043834B2 (en) | 2003-03-31 | 2011-10-25 | Qiagen Gmbh | Universal reagents for rolling circle amplification and methods of use |
| US20040248103A1 (en) * | 2003-06-04 | 2004-12-09 | Feaver William John | Proximity-mediated rolling circle amplification |
| WO2005010159A2 (en) * | 2003-07-17 | 2005-02-03 | Children's Hospital Medical Center | Rolling circle amplification of micro-rna samples |
| US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
| EP1716254B1 (de) | 2004-02-19 | 2010-04-07 | Helicos Biosciences Corporation | Verfahren zur analyse von polynukleotidsequenzen |
| JP2008506165A (ja) * | 2004-06-18 | 2008-02-28 | リール・トゥー・リミテッド | データ集合の目録作成および探索のための方法およびシステム |
| US20080096255A1 (en) * | 2004-07-02 | 2008-04-24 | Matthias Harbers | Method for Preparing Sequence Tags |
| US7170050B2 (en) | 2004-09-17 | 2007-01-30 | Pacific Biosciences Of California, Inc. | Apparatus and methods for optical analysis of molecules |
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| DK1907583T4 (da) | 2005-06-15 | 2020-01-27 | Complete Genomics Inc | Enkeltmolekyle-arrays til genetisk og kemisk analyse |
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| US8617811B2 (en) * | 2008-01-28 | 2013-12-31 | Complete Genomics, Inc. | Methods and compositions for efficient base calling in sequencing reactions |
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| US11525163B2 (en) | 2012-10-29 | 2022-12-13 | The Johns Hopkins University | Papanicolaou test for ovarian and endometrial cancers |
| JP6112652B2 (ja) * | 2012-11-29 | 2017-04-12 | 国立大学法人広島大学 | 標的ｄｎａの特異的増幅方法 |
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| CN111378568A (zh) | 2013-09-06 | 2020-07-07 | 赛拉诺斯知识产权有限责任公司 | 用于检测感染性疾病的系统和方法 |
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| US11230708B2 (en) | 2015-12-18 | 2022-01-25 | Massachusetts Institute Of Technology | Concatemeric RNA molecules, compositions, and methods and uses thereof |
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-
2002
- 2002-12-31 US US10/335,573 patent/US6977153B2/en not_active Expired - Lifetime
-
2003
- 2003-12-11 CA CA002512196A patent/CA2512196A1/en not_active Abandoned
- 2003-12-11 JP JP2004565385A patent/JP2006512081A/ja active Pending
- 2003-12-11 AU AU2003297891A patent/AU2003297891A1/en not_active Abandoned
- 2003-12-11 DE DE60332873T patent/DE60332873D1/de not_active Expired - Lifetime
- 2003-12-11 AT AT03796961T patent/ATE469983T1/de not_active IP Right Cessation
- 2003-12-11 WO PCT/US2003/039430 patent/WO2004061119A2/en active Application Filing
- 2003-12-11 EP EP03796961A patent/EP1585833B1/de not_active Expired - Lifetime
-
2005
- 2005-08-10 US US11/201,339 patent/US20060188893A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| ATE469983T1 (de) | 2010-06-15 |
| CA2512196A1 (en) | 2004-07-22 |
| EP1585833A2 (de) | 2005-10-19 |
| JP2006512081A (ja) | 2006-04-13 |
| WO2004061119A2 (en) | 2004-07-22 |
| US6977153B2 (en) | 2005-12-20 |
| AU2003297891A1 (en) | 2004-07-29 |
| US20060188893A1 (en) | 2006-08-24 |
| US20040126770A1 (en) | 2004-07-01 |
| EP1585833B1 (de) | 2010-06-02 |
| WO2004061119A3 (en) | 2004-11-11 |
| EP1585833A4 (de) | 2007-02-14 |
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