DE68914664D1 - Gereinigte ubiquitin-hydrolase, diese kodierende dns-sequenzen und ihre verwendung zur gewinnung von polypeptiden. - Google Patents

Gereinigte ubiquitin-hydrolase, diese kodierende dns-sequenzen und ihre verwendung zur gewinnung von polypeptiden.

Info

Publication number
DE68914664D1
DE68914664D1 DE68914664T DE68914664T DE68914664D1 DE 68914664 D1 DE68914664 D1 DE 68914664D1 DE 68914664 T DE68914664 T DE 68914664T DE 68914664 T DE68914664 T DE 68914664T DE 68914664 D1 DE68914664 D1 DE 68914664D1
Authority
DE
Germany
Prior art keywords
dna sequences
encoding dna
ubiquitin hydrolase
obtain polypeptides
purified ubiquitin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE68914664T
Other languages
English (en)
Other versions
DE68914664T2 (de
Inventor
Chung-Cheng Liu
Harvey Miller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/210,909 external-priority patent/US5156968A/en
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of DE68914664D1 publication Critical patent/DE68914664D1/de
Application granted granted Critical
Publication of DE68914664T2 publication Critical patent/DE68914664T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/64—Relaxins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)
DE68914664T 1988-06-24 1989-06-16 Gereinigte ubiquitin-hydrolase, diese kodierende dns-sequenzen und ihre verwendung zur gewinnung von polypeptiden. Expired - Lifetime DE68914664T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US07/210,909 US5156968A (en) 1988-06-24 1988-06-24 Purified yeast ubiquitin hydrolase
US07/284,281 US5108919A (en) 1988-06-24 1988-12-14 Dna sequences encoding yeast ubiquitin hydrolase
PCT/US1989/002651 WO1989012678A1 (en) 1988-06-24 1989-06-16 Purified ubiquitin hydrolase, dna sequences encoding same, and its use in recovering polypeptides

Publications (2)

Publication Number Publication Date
DE68914664D1 true DE68914664D1 (de) 1994-05-19
DE68914664T2 DE68914664T2 (de) 1994-10-20

Family

ID=26905634

Family Applications (1)

Application Number Title Priority Date Filing Date
DE68914664T Expired - Lifetime DE68914664T2 (de) 1988-06-24 1989-06-16 Gereinigte ubiquitin-hydrolase, diese kodierende dns-sequenzen und ihre verwendung zur gewinnung von polypeptiden.

Country Status (9)

Country Link
US (1) US5108919A (de)
EP (1) EP0420936B1 (de)
JP (1) JPH03505527A (de)
AU (1) AU619319B2 (de)
DE (1) DE68914664T2 (de)
DK (1) DK303790A (de)
ES (1) ES2018901A6 (de)
FI (1) FI906214A0 (de)
WO (1) WO1989012678A1 (de)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5196321A (en) * 1986-10-02 1993-03-23 Massachusetts Institute Of Technology Methods for in vitro cleavage of ubiquitin fusion proteins
US5093242A (en) * 1986-10-02 1992-03-03 Massachusetts Institute Of Technology Methods of generating desired amino-terminal residues in proteins
ATE106449T1 (de) * 1989-06-30 1994-06-15 Massachusetts Inst Technology Hemmung des reaktionswegs für die n-ende-regel in lebenden zellen.
US5620923A (en) * 1989-10-12 1997-04-15 The University Of Utah Synthesis of peptides as cloned ubiquitin extensions
CA2036965A1 (en) * 1990-02-26 1991-08-27 Robert J. Duronio Method for protein n-myristoylation
US5212058A (en) * 1990-05-09 1993-05-18 Massachusetts Institute Of Technology Nucleic acid encoding ubiquitin-specific proteases
EP0531404B1 (de) * 1990-05-09 1995-04-19 Massachusetts Institute Of Technology Ubiquitinspezifische protease
US5716816A (en) * 1992-05-22 1998-02-10 The United States Of America As Represented By The Department Of Health And Human Services Clones encoding mammalian ADP-ribosylarginine hydrolases
CA2146973C (en) * 1992-10-29 2008-09-02 Patricia R. Segarini Uses of tgf-.beta. receptor fragment as a therapeutic agent
US6653450B1 (en) 1993-01-28 2003-11-25 Cohesion Technologies, Inc. Mutated recombinant collagens
US5459051A (en) * 1993-03-26 1995-10-17 Celtrix Pharmaceuticals, Inc. Methods and vectors for over-expression of ubiquitin fusion proteins in host cells
US5563046A (en) * 1993-08-02 1996-10-08 Celtrix Pharmaceuticals, Inc. Fusion polypeptides and proteins
US5914254A (en) * 1993-08-02 1999-06-22 Celtrix Pharmaceuticals, Inc. Expression of fusion polypeptides transported out of the cytoplasm without leader sequences
US5744343A (en) * 1994-01-04 1998-04-28 Mitotix, Inc. Ubiquitin conjugating enzymes
US5981699A (en) * 1994-01-04 1999-11-09 Mitotix, Inc. Human ubiquitin conjugating enzyme
WO1995018974A2 (en) * 1994-01-04 1995-07-13 Mitotix, Inc. Ubiquitin conjugating enzymes
US5770720A (en) * 1995-08-30 1998-06-23 Barnes-Jewish Hospital Ubiquitin conjugating enzymes having transcriptional repressor activity
US6001619A (en) * 1995-10-04 1999-12-14 Cold Spring Harbor Laboratory Ubiquitin ligases, and uses related thereto
US6747128B2 (en) 1997-08-20 2004-06-08 Gpc Biotech, Inc. Components of ubiquitin ligase complexes, and uses related thereto
CN1297053A (zh) * 1999-11-22 2001-05-30 上海博容基因开发有限公司 一种新的多肽——人遍在蛋白c端水解酶28和编码这种多肽的多核苷酸
EP1284992A2 (de) * 2000-05-30 2003-02-26 Viridis Biotech Inc. Polyubiquitin hydrogel und dessen verwendung
US20050032683A1 (en) * 2000-10-04 2005-02-10 Amento Edward P. Methods of modulating apoptosis by administration of relaxin agonists or antagonists
WO2003004635A1 (en) 2001-07-06 2003-01-16 Lg Life Sciences Ltd. Novel aminopeptidase derived from bacillus licheniformis, gene encoding the aminopeptidase, expression vector containing the gene, transformant and method for preparation thereof
US7771970B2 (en) * 2001-07-26 2010-08-10 Advanced Protein Technologies Corporation Process for preparation of polypeptides of interest from fusion polypeptides
AU2002348205A1 (en) * 2001-12-12 2003-06-23 Eli Lilly And Company Expression system
MX2007007591A (es) * 2004-12-22 2007-07-25 Ambrx Inc Metodos para expresion y purificacion de hormona de crecimiento humano recombinante.
EP2275431A3 (de) * 2008-01-18 2011-05-25 Bio-Rad Laboratories, Inc. Erweiterte reinigung phosphorylierter und nicht phosphorylierter biomoleküle durch apatit-chromatographie

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4757013A (en) * 1983-07-25 1988-07-12 The Research Foundation Of State University Of New York Cloning vehicles for polypeptide expression in microbial hosts
US4703004A (en) * 1984-01-24 1987-10-27 Immunex Corporation Synthesis of protein with an identification peptide
US4771002A (en) * 1984-02-24 1988-09-13 Lubrizol Genetics, Inc. Transcription in plants and bacteria
EP0324789B1 (de) * 1986-10-02 2003-08-20 Massachusetts Institute Of Technology Verfahren zur regulierung der metabolischen stabilität von proteinen
EP0305500B1 (de) * 1987-03-20 1994-11-09 Creative Biomolecules, Inc. Verfahren zur reinigung von rekombinanten polypeptiden

Also Published As

Publication number Publication date
ES2018901A6 (es) 1991-05-16
US5108919A (en) 1992-04-28
EP0420936A1 (de) 1991-04-10
JPH03505527A (ja) 1991-12-05
AU3865989A (en) 1990-01-12
AU619319B2 (en) 1992-01-23
EP0420936B1 (de) 1994-04-13
FI906214A0 (fi) 1990-12-17
DK303790D0 (da) 1990-12-21
DE68914664T2 (de) 1994-10-20
DK303790A (da) 1991-02-22
WO1989012678A1 (en) 1989-12-28

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