DE68921515D1 - DNS-Sequenzierung. - Google Patents

DNS-Sequenzierung.

Info

Publication number
DE68921515D1
DE68921515D1 DE68921515T DE68921515T DE68921515D1 DE 68921515 D1 DE68921515 D1 DE 68921515D1 DE 68921515 T DE68921515 T DE 68921515T DE 68921515 T DE68921515 T DE 68921515T DE 68921515 D1 DE68921515 D1 DE 68921515D1
Authority
DE
Germany
Prior art keywords
dna
strand
primer
elongated
length
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE68921515T
Other languages
English (en)
Other versions
DE68921515T2 (de
Inventor
Stanley Tabor
Charles C Richardson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard College
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22813763&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE68921515(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Harvard College filed Critical Harvard College
Application granted granted Critical
Publication of DE68921515D1 publication Critical patent/DE68921515D1/de
Publication of DE68921515T2 publication Critical patent/DE68921515T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/803Physical recovery methods, e.g. chromatography, grinding
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/81Packaged device or kit
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00Chemistry: analytical and immunological testing
    • Y10S436/807Apparatus included in process claim, e.g. physical support structures
    • Y10S436/808Automated or kit
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/14Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333Saccharide [e.g., DNA, etc.]
DE68921515T 1988-07-12 1989-07-06 DNS-Sequenzierung. Expired - Lifetime DE68921515T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/218,103 US4962020A (en) 1988-07-12 1988-07-12 DNA sequencing

Publications (2)

Publication Number Publication Date
DE68921515D1 true DE68921515D1 (de) 1995-04-13
DE68921515T2 DE68921515T2 (de) 1995-07-13

Family

ID=22813763

Family Applications (2)

Application Number Title Priority Date Filing Date
DE198989306877T Pending DE351138T1 (de) 1988-07-12 1989-07-06 Dns-sequenzierung.
DE68921515T Expired - Lifetime DE68921515T2 (de) 1988-07-12 1989-07-06 DNS-Sequenzierung.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE198989306877T Pending DE351138T1 (de) 1988-07-12 1989-07-06 Dns-sequenzierung.

Country Status (17)

Country Link
US (4) US4962020A (de)
EP (1) EP0351138B1 (de)
JP (1) JP2870696B2 (de)
KR (1) KR900001860A (de)
CN (1) CN1039845A (de)
AT (1) ATE119579T1 (de)
AU (1) AU614245B2 (de)
CA (1) CA1340851C (de)
DD (1) DD284052A5 (de)
DE (2) DE351138T1 (de)
DK (1) DK342289A (de)
ES (1) ES2015853A4 (de)
GR (1) GR900300159T1 (de)
HU (1) HUT52543A (de)
IL (1) IL90850A0 (de)
LT (1) LTIP1515A (de)
NO (1) NO892825L (de)

Families Citing this family (163)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4962020A (en) * 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
US5075216A (en) * 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
AU7167191A (en) * 1989-11-28 1991-06-26 United States Biochemical Corporation High resolution scanner
US6013431A (en) 1990-02-16 2000-01-11 Molecular Tool, Inc. Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators
US5888819A (en) * 1991-03-05 1999-03-30 Molecular Tool, Inc. Method for determining nucleotide identity through primer extension
US6004744A (en) * 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
AU660484B2 (en) * 1991-07-24 1995-06-29 University Partnerships Pty Ltd Single step amplification and sequencing of nucleic acids
WO1993002212A1 (en) * 1991-07-24 1993-02-04 University Partnerships Pty. Ltd. Single step amplification and sequencing of nucleic acids
EP0535587A1 (de) * 1991-09-30 1993-04-07 Beckman Instruments, Inc. Verfahren für die Verbesserung von Nucleotid-Sequenz-Feststellung
US5273638A (en) * 1991-09-30 1993-12-28 Beckman Instruments, Inc. Nucleotide sequence determination employing matched dideoxynucleotide terminator concentrations
JP2821050B2 (ja) * 1991-10-31 1998-11-05 浜松ホトニクス株式会社 核酸塩基識別法
USH1985H1 (en) 1992-01-09 2001-08-07 The United States Of America As Represented By The Secretary Of The Navy Method for detecting biological toxins
US5213669A (en) * 1992-01-31 1993-05-25 Beckman Instruments, Inc. Capillary column containing a dynamically cross-linked composition and method of use
GB9210176D0 (en) 1992-05-12 1992-06-24 Cemu Bioteknik Ab Chemical method
US5516664A (en) * 1992-12-23 1996-05-14 Hyman; Edward D. Enzymatic synthesis of repeat regions of oligonucleotides
US5500339A (en) * 1993-03-30 1996-03-19 Amersham Life Science, Inc. DNA denaturation method
US5547859A (en) * 1993-08-02 1996-08-20 Goodman; Myron F. Chain-terminating nucleotides for DNA sequencing methods
JP3419850B2 (ja) * 1993-10-26 2003-06-23 株式会社日立製作所 核酸分別方法
US5545527A (en) * 1994-07-08 1996-08-13 Visible Genetics Inc. Method for testing for mutations in DNA from a patient sample
US5834189A (en) * 1994-07-08 1998-11-10 Visible Genetics Inc. Method for evaluation of polymorphic genetic sequences, and the use thereof in identification of HLA types
US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
EP0655506B1 (de) * 1994-10-17 1996-09-18 President And Fellows Of Harvard College DNS Polymerase mit veränderter Nukleotid-Bindungstelle
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
US5861296A (en) * 1994-10-25 1999-01-19 New England Biolabs, Inc. Purified thermostable inorganic pyprophosphatase obtainable from thermococcus litoralis
US5627022A (en) * 1994-11-01 1997-05-06 Visible Genetics Inc. Microgels for use in medical diagnosis and holders useful in fabricating same
US5507934A (en) * 1994-11-01 1996-04-16 Visible Genetics Inc. Apparatus for preparing gels for use in electrophoretic separations and similar applications
US5710628A (en) * 1994-12-12 1998-01-20 Visible Genetics Inc. Automated electrophoresis and fluorescence detection apparatus and method
US6014213A (en) * 1994-12-12 2000-01-11 Visible Genetics Inc. High dynamic range apparatus for separation and detection of polynucleotide fragments
US5543018A (en) * 1995-02-13 1996-08-06 Visible Genetics Inc. Method and apparatus for automated electrophoresis using light polarization detector
WO1996036737A1 (en) * 1995-05-19 1996-11-21 Ely Michael Rabani Parallel multiplex polynucleotide sequencing
US6077664A (en) * 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US6001645A (en) * 1995-06-07 1999-12-14 Promega Corporation Thermophilic DNA polymerases from thermotoga neapolitana
US5948614A (en) * 1995-09-08 1999-09-07 Life Technologies, Inc. Cloned DNA polymerases from thermotoga maritima and mutants thereof
US5599434A (en) * 1995-12-12 1997-02-04 Visible Genetics Inc. Electrophoresis gels and gel holders having adhesive affixed fiber spacers and method of making same
US5618398A (en) * 1995-12-12 1997-04-08 Visible Genetics Inc. Electrophoresis gels and gel holders having fiber spacers and method of making same
US6007983A (en) * 1995-12-22 1999-12-28 Visible Genetics Inc. Method and kit for evaluation of HIV mutations
US5888736A (en) * 1995-12-22 1999-03-30 Visible Genetics, Inc. Method, compositions and kit for detection and identification of microorganisms
US6265152B1 (en) 1995-12-22 2001-07-24 Visible Genetics Inc. Method and kit for evaluation of HIV mutations
US6613508B1 (en) * 1996-01-23 2003-09-02 Qiagen Genomics, Inc. Methods and compositions for analyzing nucleic acid molecules utilizing sizing techniques
US6312893B1 (en) * 1996-01-23 2001-11-06 Qiagen Genomics, Inc. Methods and compositions for determining the sequence of nucleic acid molecules
US6027890A (en) * 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
AU2320597A (en) 1996-03-19 1997-10-10 Molecular Tool, Inc. Method for determining the nucleotide sequence of a polynucleotide
US6043036A (en) * 1996-04-23 2000-03-28 Aclara Biosciences Method of sequencing nucleic acids by shift registering
US6214555B1 (en) * 1996-05-01 2001-04-10 Visible Genetics Inc. Method compositions and kit for detection
US6413718B1 (en) 1996-05-01 2002-07-02 Visible Genetics Inc. Method for sequencing of nucleic acid polymers
US5871929A (en) * 1996-07-23 1999-02-16 Barnes; Wayne M. Suppression of pyrophosphorolysis in DNA sequencing and in other applications involving DNA replication
EP0942917B1 (de) 1996-08-14 2015-02-25 Life Technologies Corporation Verwendung von stabile zubereitungen in nukleinsäureamplifikation und sequenzierung verfahren
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
WO1998015655A1 (en) * 1996-10-07 1998-04-16 The Perkin-Elmer Corporation Primer extension reaction utilizing a cosubstrate-enzyme pair for consuming pyrophosphate
US6291164B1 (en) * 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
US6828094B2 (en) * 1996-12-20 2004-12-07 Roche Diagnostics Gmbh Method for the uncoupled, direct, exponential amplification and sequencing of DNA molecules with the addition of a second thermostable DNA polymerase and its application
US6399304B1 (en) 1996-12-20 2002-06-04 Roche Diagnostics Gmbh Sequential activation of one or more enzymatic activities within a thermocycling reaction for synthesizing DNA molecules
US20030215857A1 (en) * 1996-12-20 2003-11-20 Roche Diagnostics Gmbh Method for the direct, exponential amplification and sequencing of DNA molecules and its application
US6605428B2 (en) 1996-12-20 2003-08-12 Roche Diagnostics Gmbh Method for the direct, exponential amplification and sequencing of DNA molecules and its application
DE19653439A1 (de) * 1996-12-20 1998-07-02 Svante Dr Paeaebo Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung
DE19653494A1 (de) * 1996-12-20 1998-06-25 Svante Dr Paeaebo Verfahren zur entkoppelten, direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen unter Zugabe einer zweiten thermostabilen DNA Polymerase und dessen Anwendung
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US6306588B1 (en) * 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
CA2281205A1 (en) 1997-02-12 1998-08-13 Eugene Y. Chan Methods and products for analyzing polymers
US6117634A (en) * 1997-03-05 2000-09-12 The Reagents Of The University Of Michigan Nucleic acid sequencing and mapping
US6830887B2 (en) 1997-03-18 2004-12-14 Bayer Healthcare Llc Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample
CA2302393A1 (en) 1997-08-29 1999-03-04 Life Technologies, Inc. High fidelity polymerases and uses thereof
US7632651B2 (en) 1997-09-15 2009-12-15 Mds Analytical Technologies (Us) Inc. Molecular modification assays
US7745142B2 (en) 1997-09-15 2010-06-29 Molecular Devices Corporation Molecular modification assays
US7070921B2 (en) 2000-04-28 2006-07-04 Molecular Devices Corporation Molecular modification assays
US6982431B2 (en) 1998-08-31 2006-01-03 Molecular Devices Corporation Sample analysis systems
US6297018B1 (en) 1998-04-17 2001-10-02 Ljl Biosystems, Inc. Methods and apparatus for detecting nucleic acid polymorphisms
US6787305B1 (en) 1998-03-13 2004-09-07 Invitrogen Corporation Compositions and methods for enhanced synthesis of nucleic acid molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
AU5008900A (en) * 1999-05-12 2000-11-21 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
KR100430310B1 (ko) * 1999-11-26 2004-05-10 (주)바이오니아 다양한 핵산 중합효소를 사용하는 핵산 염기 서열분석방법 및 이에 사용되는 키트
US7435392B2 (en) * 2000-02-03 2008-10-14 Acclavis, Llc Scalable continuous production system
US7413714B1 (en) 2000-07-16 2008-08-19 Ymc Co. Ltd. Sequential reaction system
DE10036602A1 (de) * 2000-07-27 2002-02-14 Cpc Cellular Process Chemistry Mikroreaktor für Reaktionen zwischen Gasen und Flüssigkeiten
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6548251B1 (en) 2000-09-05 2003-04-15 Fidelity Systems, Inc. Inhibition of molecular and biological processes using modified oligonucleotides
US6835537B1 (en) 2000-09-29 2004-12-28 Myriad Genetics, Inc. Method for equalizing band intensities on sequencing gels
DE10054974A1 (de) * 2000-11-06 2002-06-06 Epigenomics Ag Diagnose von mit Cdk4 assoziierten Krankheiten
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
CA2440754A1 (en) 2001-03-12 2002-09-19 Stephen Quake Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US6733485B1 (en) 2001-05-25 2004-05-11 Advanced Bionics Corporation Microstimulator-based electrochemotherapy methods and systems
EP1421175B1 (de) * 2001-06-28 2008-11-19 Mountain View Pharmaceuticals, Inc. Polymerstabilisierte proteinasen
CA2460546A1 (en) * 2001-09-14 2003-03-27 Invitrogen Corporation Dna polymerases and mutants thereof
US7222059B2 (en) * 2001-11-15 2007-05-22 Siemens Medical Solutions Diagnostics Electrophoretic trace simulator
EP2345738A1 (de) 2002-08-05 2011-07-20 Quanta Biosciences, Inc. Verbesserte Zusammensetzung zur In-vitro-Amplifikation von Nukleinsäuren
US20040126765A1 (en) * 2002-12-27 2004-07-01 Adams Craig W. Method and compositions for sequencing nucleic acid molecules
EP1618372A2 (de) * 2003-04-14 2006-01-25 Cellular Process Chemistry, Inc. System und verfahren zur bestimmung der optimalen reaktionsparameter unter verwendung eines kontinuierlich laufenden prozesses
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
EP1697534B1 (de) 2003-12-01 2010-06-02 Life Technologies Corporation Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
US20060141487A1 (en) * 2004-01-26 2006-06-29 Applera Corporation Methods, compositions, and kits for amplifying and sequencing polynucleotides
EP2248911A1 (de) 2004-02-19 2010-11-10 Helicos Biosciences Corporation Verfahren und Zusammensetzungen zur Analyse von Polynukleotidsequenzen
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US20050239085A1 (en) * 2004-04-23 2005-10-27 Buzby Philip R Methods for nucleic acid sequence determination
EP1751314B1 (de) * 2004-05-13 2019-09-18 Nanobiosym, Inc. Nano-pcr: verfahren und vorrichtung zur amplifikation und detektion von nukleinsäuren
US20050260609A1 (en) * 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
WO2006007207A2 (en) 2004-05-25 2006-01-19 Helicos Biosciences Corporation Methods and devices for nucleic acid sequence determination
US7476734B2 (en) * 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
WO2006086673A2 (en) * 2005-02-09 2006-08-17 Pacific Biosciences Of California, Inc. Nucleotide compositions and uses thereof
US20060292578A1 (en) * 2005-06-28 2006-12-28 Weidong Zheng DNA polymerase blends and mutant DNA polymerases
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7998717B2 (en) * 2005-12-02 2011-08-16 Pacific Biosciences Of California, Inc. Mitigation of photodamage in analytical reactions
US20090305248A1 (en) * 2005-12-15 2009-12-10 Lander Eric G Methods for increasing accuracy of nucleic acid sequencing
US7397546B2 (en) * 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US20080309926A1 (en) * 2006-03-08 2008-12-18 Aaron Weber Systems and methods for reducing detected intensity non uniformity in a laser beam
CN101479605A (zh) 2006-04-21 2009-07-08 纳诺拜希姆公司 用于药物发现的单分子平台:用于包括抗癌和抗病毒剂的发现的药物发现的方法和装置
EP1914303A1 (de) * 2006-10-09 2008-04-23 Qiagen GmbH Thermus eggertssonii DNA Polymerasen
WO2009045344A2 (en) * 2007-09-28 2009-04-09 Pacific Biosciences Of California, Inc. Error-free amplification of dna for clonal sequencing
US7960116B2 (en) 2007-09-28 2011-06-14 Pacific Biosciences Of California, Inc. Nucleic acid sequencing methods and systems
US20090203086A1 (en) * 2008-02-06 2009-08-13 454 Life Sciences Corporation System and method for improved signal detection in nucleic acid sequencing
US8652781B2 (en) 2008-02-12 2014-02-18 Pacific Biosciences Of California, Inc. Cognate sampling kinetics
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
WO2009120374A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
WO2009120372A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
US20090247426A1 (en) * 2008-03-31 2009-10-01 Pacific Biosciences Of California, Inc. Focused library generation
EP2274446B1 (de) 2008-03-31 2015-09-09 Pacific Biosciences of California, Inc. Systeme und verfahren für polymeraseenzymreaktionen in zwei langsamen schritten
US8420366B2 (en) * 2008-03-31 2013-04-16 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
ES2614078T3 (es) 2008-03-31 2017-05-29 Pacific Biosciences Of California, Inc. Generación de polimerasas modificadas para precisión mejorada en secuenciación de una única molécula
EP3629022A1 (de) 2008-07-25 2020-04-01 Richard W. Wagner Proteinscreeningverfahren
WO2010027497A2 (en) * 2008-09-05 2010-03-11 Pacific Biosciences Of California, Inc Preparations, compositions, and methods for nucleic acid sequencing
AU2009288696A1 (en) * 2008-09-05 2010-03-11 Pacific Biosciences Of California, Inc. Sequencing by cognate sampling
WO2010036287A1 (en) * 2008-09-24 2010-04-01 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US8383369B2 (en) * 2008-09-24 2013-02-26 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
WO2012009206A2 (en) 2010-07-12 2012-01-19 Pacific Biosciences Of California, Inc. Sequencing reactions with alkali metal cations for pulse width control
WO2012018638A2 (en) 2010-07-26 2012-02-09 Biomatrica, Inc. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
US9845489B2 (en) 2010-07-26 2017-12-19 Biomatrica, Inc. Compositions for stabilizing DNA, RNA and proteins in saliva and other biological samples during shipping and storage at ambient temperatures
US8834847B2 (en) 2010-08-12 2014-09-16 Pacific Biosciences Of California, Inc. Photodamage mitigation compounds and systems
EP2689028B1 (de) 2011-03-23 2017-08-30 Pacific Biosciences Of California, Inc. Isolierung von polymerase-nukleinsäurekomplexen und deren ladung auf substrate
US9803239B2 (en) 2012-03-29 2017-10-31 Complete Genomics, Inc. Flow cells for high density array chips
WO2013185137A1 (en) 2012-06-08 2013-12-12 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
US9399766B2 (en) 2012-10-01 2016-07-26 Pacific Biosciences Of California, Inc. Recombinant polymerases for incorporation of protein shield nucleotide analogs
US9725703B2 (en) 2012-12-20 2017-08-08 Biomatrica, Inc. Formulations and methods for stabilizing PCR reagents
US10933417B2 (en) 2013-03-15 2021-03-02 Nanobiosym, Inc. Systems and methods for mobile device analysis of nucleic acids and proteins
EP3351645B1 (de) 2013-05-24 2020-07-15 Illumina Cambridge Limited Pyrophosphorolytische sequenzierung mit nanoporen
WO2015191632A1 (en) 2014-06-10 2015-12-17 Biomatrica, Inc. Stabilization of thrombocytes at ambient temperatures
US10302972B2 (en) 2015-01-23 2019-05-28 Pacific Biosciences Of California, Inc. Waveguide transmission
EP3350350B1 (de) 2015-11-19 2021-04-14 Pacific Biosciences of California, Inc. Verbindungen und systeme zur verbesserung der signaldetektion
WO2017100212A1 (en) 2015-12-08 2017-06-15 Biomatrica, Inc. Reduction of erythrocyte sedimentation rate
MA45491A (fr) 2016-06-27 2019-05-01 Juno Therapeutics Inc Épitopes à restriction cmh-e, molécules de liaison et procédés et utilisations associés
WO2018005559A1 (en) 2016-06-27 2018-01-04 Juno Therapeutics, Inc. Method of identifying peptide epitopes, molecules that bind such epitopes and related uses
WO2018213787A1 (en) 2017-05-18 2018-11-22 Ultima Genomics, Inc. Sequencing using non-natural nucleotides
SG11201912282YA (en) 2017-06-21 2020-01-30 Base4 Innovation Ltd Microdroplet manipulation device
SG10202111850XA (en) * 2017-06-21 2021-12-30 Lightcast Discovery Ltd Method for Investigating Molecules Such as Nucleic Acids
EP3641935B1 (de) 2017-06-21 2021-09-08 Lightcast Discovery Ltd Gerät zur untersuchung von nukleinsäuren
US11851679B2 (en) 2017-11-01 2023-12-26 Juno Therapeutics, Inc. Method of assessing activity of recombinant antigen receptors
US11499962B2 (en) 2017-11-17 2022-11-15 Ultima Genomics, Inc. Methods and systems for analyte detection and analysis
US10344328B2 (en) 2017-11-17 2019-07-09 Ultima Genomics, Inc. Methods for biological sample processing and analysis
EP3775259A4 (de) 2018-03-26 2022-01-05 Ultima Genomics, Inc. Verfahren zur sequenzierung von nukleinsäuremolekülen
US11649493B1 (en) 2018-07-31 2023-05-16 Ultima Genomics, Inc. Methods and systems for nucleic acid sequencing
US10512911B1 (en) 2018-12-07 2019-12-24 Ultima Genomics, Inc. Implementing barriers for controlled environments during sample processing and detection
CA3224572A1 (en) 2019-02-19 2020-08-27 Ultima Genomics, Inc. Linkers and methods for optical detection and sequencing
EP3939047A4 (de) 2019-03-10 2022-11-30 Ultima Genomics, Inc. Verfahren und systeme für sequenz-calling
US11118223B2 (en) 2019-03-14 2021-09-14 Ultima Genomics, Inc. Methods, devices, and systems for analyte detection and analysis
CA3129726A1 (en) 2019-03-14 2020-09-17 Nathan Beckett Methods, devices, and systems for analyte detection and analysis
US10900078B2 (en) 2019-03-14 2021-01-26 Ultima Genomics, Inc. Methods, devices, and systems for analyte detection and analysis
US10852518B1 (en) 2019-03-14 2020-12-01 Ultima Genomics, Inc. Methods, devices, and systems for analyte detection and analysis
US10830703B1 (en) 2019-03-14 2020-11-10 Ultima Genomics, Inc. Methods, devices, and systems for analyte detection and analysis
CA3136034A1 (en) 2019-05-03 2020-11-12 Florian OBERSTRASS Methods of sequencing nucleic acid molecules
US11807851B1 (en) 2020-02-18 2023-11-07 Ultima Genomics, Inc. Modified polynucleotides and uses thereof

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3661893A (en) * 1968-11-25 1972-05-09 Univ Illinois Synthesis in vitro of nucleic acids and products used therein and products produced therefrom
US4072574A (en) * 1976-07-30 1978-02-07 The Institute For Cancer Research System for in vitro assay for mutagenicity and/or carcinogenicity of chemicals or other exogenous agents
EP0086053B1 (de) * 1982-01-26 1986-01-02 Imahori Kazutomo Verfahren zur Herstellung von Peptiden oder Peptide-Derivaten
US4729947A (en) * 1984-03-29 1988-03-08 The Board Of Regents Of The University Of Nebraska DNA sequencing
US4870004A (en) * 1984-06-08 1989-09-26 Autoseq, Inc. Apparatus and method of analyzing nucleic acid molecules
US4795700A (en) * 1985-01-25 1989-01-03 California Institute Of Technology Nucleic acid probes and methods of using same
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4865968A (en) * 1985-04-01 1989-09-12 The Salk Institute For Biological Studies DNA sequencing
US4863849A (en) * 1985-07-18 1989-09-05 New York Medical College Automatable process for sequencing nucleotide
US4707235A (en) * 1985-10-16 1987-11-17 Pharmacia, Inc. Electrophoresis method and apparatus having continuous detection means
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US4811218A (en) * 1986-06-02 1989-03-07 Applied Biosystems, Inc. Real time scanning electrophoresis apparatus for DNA sequencing
CA1338457C (en) * 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
JPS63115062A (ja) * 1986-11-04 1988-05-19 Hitachi Ltd Dnaの塩基配列決定装置
JPS63149563A (ja) * 1986-12-12 1988-06-22 Hitachi Ltd 核酸塩基配列決定装置
US4795699A (en) * 1987-01-14 1989-01-03 President And Fellows Of Harvard College T7 DNA polymerase
US4942130A (en) * 1987-01-14 1990-07-17 President & Fellows Of Harvard College T7 DNA polymerase
US4971671A (en) * 1987-03-02 1990-11-20 Xerox Corporation Processes for separation of DNA fragments
US4971903A (en) * 1988-03-25 1990-11-20 Edward Hyman Pyrophosphate-based method and apparatus for sequencing nucleic acids
US4962020A (en) * 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
DE3841565C2 (de) * 1988-12-09 1998-07-09 Europ Lab Molekularbiolog Verfahren zur Sequenzierung von Nukleinsäuren

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US5409811A (en) 1995-04-25
EP0351138A2 (de) 1990-01-17
DE351138T1 (de) 1990-11-29
ATE119579T1 (de) 1995-03-15
US5674716A (en) 1997-10-07
ES2015853A4 (es) 1990-09-16
NO892825D0 (no) 1989-07-07
IL90850A0 (en) 1990-02-09
CN1039845A (zh) 1990-02-21
US5122345A (en) 1992-06-16
HUT52543A (en) 1990-07-28
DD284052A5 (de) 1990-10-31
AU3798689A (en) 1990-01-18
DK342289D0 (da) 1989-07-11
CA1340851C (en) 1999-12-14
KR900001860A (ko) 1990-02-27
DE68921515T2 (de) 1995-07-13
DK342289A (da) 1990-01-15
EP0351138B1 (de) 1995-03-08
JP2870696B2 (ja) 1999-03-17
US4962020A (en) 1990-10-09
LTIP1515A (en) 1995-06-26
NO892825L (no) 1990-01-15
EP0351138A3 (de) 1991-12-18
AU614245B2 (en) 1991-08-22
JPH02154699A (ja) 1990-06-14
GR900300159T1 (en) 1991-09-27

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