DE69105959D1 - Verfahren zur unterscheidung von nukleinsäuren auf basis von nukleotidverschiedenheiten. - Google Patents

Verfahren zur unterscheidung von nukleinsäuren auf basis von nukleotidverschiedenheiten.

Info

Publication number
DE69105959D1
DE69105959D1 DE69105959T DE69105959T DE69105959D1 DE 69105959 D1 DE69105959 D1 DE 69105959D1 DE 69105959 T DE69105959 T DE 69105959T DE 69105959 T DE69105959 T DE 69105959T DE 69105959 D1 DE69105959 D1 DE 69105959D1
Authority
DE
Germany
Prior art keywords
extension product
nucleic acids
nucleotide differences
extension
acids based
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69105959T
Other languages
English (en)
Other versions
DE69105959T2 (de
Inventor
Kenneth James Livak
Jan Antoni Rafalski
Scott Valray Tingey
John Gregory Kelly Williams
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23963743&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69105959(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Application granted granted Critical
Publication of DE69105959D1 publication Critical patent/DE69105959D1/de
Publication of DE69105959T2 publication Critical patent/DE69105959T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
DE69105959T 1990-03-14 1991-02-12 Verfahren zur unterscheidung von nukleinsäuren auf basis von nukleotidverschiedenheiten. Expired - Fee Related DE69105959T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/494,258 US5126239A (en) 1990-03-14 1990-03-14 Process for detecting polymorphisms on the basis of nucleotide differences
PCT/US1991/000841 WO1991014001A2 (en) 1990-03-14 1991-02-12 A process for distinguishing nucleic acids on the basis of nucleotide differences

Publications (2)

Publication Number Publication Date
DE69105959D1 true DE69105959D1 (de) 1995-01-26
DE69105959T2 DE69105959T2 (de) 1995-05-18

Family

ID=23963743

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69105959T Expired - Fee Related DE69105959T2 (de) 1990-03-14 1991-02-12 Verfahren zur unterscheidung von nukleinsäuren auf basis von nukleotidverschiedenheiten.

Country Status (17)

Country Link
US (1) US5126239A (de)
EP (1) EP0520039B1 (de)
JP (1) JP2818486B2 (de)
AT (1) ATE115634T1 (de)
AU (1) AU639565B2 (de)
BR (1) BR9106151A (de)
CA (1) CA2078132C (de)
DE (1) DE69105959T2 (de)
DK (1) DK0520039T3 (de)
ES (1) ES2065684T3 (de)
FI (1) FI924049A0 (de)
IL (1) IL97503A (de)
NO (1) NO304655B1 (de)
NZ (1) NZ237396A (de)
PL (1) PL166895B1 (de)
WO (1) WO1991014001A2 (de)
ZA (1) ZA911891B (de)

Families Citing this family (101)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5595870A (en) * 1989-02-17 1997-01-21 The Trustees Of Columbia University In The City Of New York Identifying nucleic acids by restriction digestion and hybridization with random or pseudorandom oligonucleotides
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
JPH06500471A (ja) * 1990-08-24 1994-01-20 ザ・ユニバーシティ・オブ・テネシー・リサーチ・コーポレーション Ｄｎａ増幅フィンガープリント法
US6074818A (en) * 1990-08-24 2000-06-13 The University Of Tennessee Research Corporation Fingerprinting of nucleic acids, products and methods
WO1992004458A1 (en) * 1990-09-11 1992-03-19 The Brookdale Hospital Medical Center Drug resistance screening method
WO1992007095A1 (en) * 1990-10-15 1992-04-30 Stratagene Arbitrarily primed polymerase chain reaction method for fingerprinting genomes
US5635354A (en) * 1991-01-09 1997-06-03 Institut National De La Sante Et De La Recherche Medicale (Inserm) Method for describing the repertoires of antibodies (Ab) and of T-cell receptors (TcR) of an individual's immune system
US5401630A (en) * 1991-05-03 1995-03-28 The United States Of America As Represented By The Secretary Of Agriculture Species-specific DNA-DNA hybridization probe prepared using chromosome size DNA
CA2077135A1 (en) * 1991-08-30 1993-03-01 Joh-E Ikeda A method of dna amplification
US20100267023A1 (en) 1992-09-24 2010-10-21 Keygene N.V. Selective restriction fragment amplification: fingerprinting
KR100321510B1 (ko) * 1991-09-24 2005-01-10 키진 엔.브이. 선택적인 제한단편의 증폭:디엔에이(dna) 핑거프린트법
US7026115B1 (en) 1991-09-24 2006-04-11 Keygene N.V. Selective restriction fragment amplification: fingerprinting
US5665544A (en) * 1992-05-27 1997-09-09 Amersham International Plc RNA fingerprinting to determine RNA population differences
US5340728A (en) * 1992-12-09 1994-08-23 E. I. Du Pont De Nemours And Company Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction
US5962221A (en) * 1993-01-19 1999-10-05 Univ Tennessee Res Corp Oligonucleotide constructs and methods for the generation of sequence signatures from nucleic acids
EP0695366A4 (de) * 1993-04-16 1999-07-28 F B Investments Pty Ltd Methode bezüglich zufällig amplifizierter polymorpher dna (rapd)
AU1682595A (en) * 1994-01-21 1995-08-08 North Carolina State University Methods for within family selection in woody perennials using genetic markers
WO1995021269A1 (en) * 1994-02-04 1995-08-10 Perlin Mark K Method and apparatus for analyzing genetic material
US6037127A (en) * 1994-03-31 2000-03-14 E. I. Du Pont De Nemours And Company Method for detection of non-denatured nucleic acid fragments
US5552278A (en) * 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5714330A (en) * 1994-04-04 1998-02-03 Lynx Therapeutics, Inc. DNA sequencing by stepwise ligation and cleavage
US5831065A (en) * 1994-04-04 1998-11-03 Lynx Therapeutics, Inc. Kits for DNA sequencing by stepwise ligation and cleavage
US5547861A (en) * 1994-04-18 1996-08-20 Becton, Dickinson And Company Detection of nucleic acid amplification
US5585238A (en) * 1994-04-25 1996-12-17 Ciba-Geigy Corporation Detection of fungal pathogens using the polymerase chain reaction
US5660981A (en) * 1994-06-06 1997-08-26 E. I. Du Pont De Nemours And Company Selection of diagnostic genetic markers in microorganisms and use of a specific marker for detection of salmonella
US6013445A (en) * 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
USRE43097E1 (en) 1994-10-13 2012-01-10 Illumina, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
WO1996017082A2 (en) * 1994-11-28 1996-06-06 E.I. Du Pont De Nemours And Company Compound microsatellite primers for the detection of genetic polymorphisms
ES2097701B1 (es) * 1995-02-09 1997-11-16 Consejo Superior Investigacion Procedimiento para obtener sondas especificas de estirpe o de especie.
JPH09149799A (ja) * 1995-11-30 1997-06-10 Hitachi Ltd 核酸の分析方法又は検査方法、及び核酸の分析装置又は検査装置
US5670325A (en) * 1996-08-14 1997-09-23 Exact Laboratories, Inc. Method for the detection of clonal populations of transformed cells in a genomically heterogeneous cellular sample
US5741650A (en) * 1996-01-30 1998-04-21 Exact Laboratories, Inc. Methods for detecting colon cancer from stool samples
US5747257A (en) * 1996-02-29 1998-05-05 E. I. Du Pont De Nemours And Company Genetic markers and methods for the detection of escherichia coli serotype-0157:H7
US6251691B1 (en) 1996-04-25 2001-06-26 Bioarray Solutions, Llc Light-controlled electrokinetic assembly of particles near surfaces
US5811239A (en) * 1996-05-13 1998-09-22 Frayne Consultants Method for single base-pair DNA sequence variation detection
US6146828A (en) * 1996-08-14 2000-11-14 Exact Laboratories, Inc. Methods for detecting differences in RNA expression levels and uses therefor
US6020137A (en) * 1996-08-14 2000-02-01 Exact Laboratories, Inc. Methods for the detection of loss of heterozygosity
US5952178A (en) 1996-08-14 1999-09-14 Exact Laboratories Methods for disease diagnosis from stool samples
US6100029A (en) * 1996-08-14 2000-08-08 Exact Laboratories, Inc. Methods for the detection of chromosomal aberrations
US5928870A (en) * 1997-06-16 1999-07-27 Exact Laboratories, Inc. Methods for the detection of loss of heterozygosity
US6300077B1 (en) 1996-08-14 2001-10-09 Exact Sciences Corporation Methods for the detection of nucleic acids
US6203993B1 (en) 1996-08-14 2001-03-20 Exact Science Corp. Methods for the detection of nucleic acids
US5814453A (en) * 1996-10-15 1998-09-29 Novartis Finance Corporation Detection of fungal pathogens using the polymerase chain reaction
US5800997A (en) * 1996-11-01 1998-09-01 Novartis Finance Corporation Detection of maize fungal pathogens using the polymerase chain reaction
US5922538A (en) * 1996-11-08 1999-07-13 E.I. Du Pont De Nemours And Company Genetic markers and methods for the detection of Listeria monocytogenes and Listeria spp
US6268136B1 (en) 1997-06-16 2001-07-31 Exact Science Corporation Methods for stool sample preparation
US6406857B1 (en) 1997-06-16 2002-06-18 Exact Sciences Corporation Methods for stool sample preparation
US5827695A (en) * 1997-08-04 1998-10-27 Novartis Finance Corporation Detection of wheat fungal pathogens using the polymerase chain reaction
US6173525B1 (en) 1997-09-19 2001-01-16 Cantharellus Ab Chanterelle mycelium
US20020064792A1 (en) * 1997-11-13 2002-05-30 Lincoln Stephen E. Database for storage and analysis of full-length sequences
EP1051523A2 (de) * 1998-02-02 2000-11-15 Amersham Pharmacia Biotech AB Nuklein-säure analyse verfahren
US7078224B1 (en) 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
US8337753B2 (en) 1998-05-01 2012-12-25 Gen-Probe Incorporated Temperature-controlled incubator having a receptacle mixing mechanism
EP1614475B1 (de) 1998-05-01 2007-05-30 Gen-Probe Incorporated Rührvorrichtung für den fluiden Inhalt eines Behälters
US6284466B1 (en) 1998-09-18 2001-09-04 The Board Of Regents Of University Of Nebraska Method of detecting genetic polymorphisms using over represented sequences
US6703228B1 (en) 1998-09-25 2004-03-09 Massachusetts Institute Of Technology Methods and products related to genotyping and DNA analysis
EP1001037A3 (de) * 1998-09-28 2003-10-01 Whitehead Institute For Biomedical Research Vorselektion und Isolierung eines Einzelnukleotidpolymorphismus
KR20010090879A (ko) * 1998-12-08 2001-10-19 추후제출 대장균주 오157:에이치7을 타 균주와 구별하는 다형성유전자좌
US6503718B2 (en) 1999-01-10 2003-01-07 Exact Sciences Corporation Methods for detecting mutations using primer extension for detecting disease
US6280947B1 (en) 1999-08-11 2001-08-28 Exact Sciences Corporation Methods for detecting nucleotide insertion or deletion using primer extension
CA2362251C (en) 1999-02-25 2008-06-17 Exact Sciences Corporation Methods for preserving dna integrity
FR2792000B1 (fr) * 1999-04-08 2003-04-11 Centre Nat Rech Scient Procede de cartographie d'une molecule d'adn comprenant une etape d'amplification ad infinitum
AU767983B2 (en) * 1999-04-09 2003-11-27 Esoterix Genetic Laboratories, Llc Methods for detecting nucleic acids indicative of cancer
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US6586177B1 (en) * 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
US6919174B1 (en) * 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
JP2003516138A (ja) * 1999-12-07 2003-05-13 エグザクト サイエンシーズ コーポレイション 結腸上好気性消化新生物の検出
US6242191B1 (en) * 1999-12-30 2001-06-05 The Ohio State University Research Foundation Methods for assessing the beef characteristics of live cattle
NZ521626A (en) * 2000-03-29 2005-09-30 Cambia Methods for genotyping by hybridization analysis
US9709559B2 (en) 2000-06-21 2017-07-18 Bioarray Solutions, Ltd. Multianalyte molecular analysis using application-specific random particle arrays
EP1311839B1 (de) 2000-06-21 2006-03-01 Bioarray Solutions Ltd Multianalytische molekularanalyse durch verwendung anwendungsspezifischer zufallspartikelarrays
US7695901B2 (en) * 2000-07-26 2010-04-13 North Carolina State University Identification of poinsettia cultivars
EP1207209A3 (de) * 2000-11-09 2002-09-11 Agilent Technologies, Inc. (a Delaware corporation) Verfahren zum Nachweis von Einzelnukleotidpolymorphismen auf Arrays
US6428964B1 (en) 2001-03-15 2002-08-06 Exact Sciences Corporation Method for alteration detection
US7262063B2 (en) 2001-06-21 2007-08-28 Bio Array Solutions, Ltd. Directed assembly of functional heterostructures
US20040002073A1 (en) 2001-10-15 2004-01-01 Li Alice Xiang Multiplexed analysis of polymorphic loci by concurrent interrogation and enzyme-mediated detection
AU2002360537A1 (en) * 2001-12-10 2003-06-23 Iowa State University Research Foundation, Inc. Nucleic acids related to plant retroelements
AU2003213107A1 (en) * 2002-02-15 2003-09-09 Exact Sciences Corporation Methods for analysis of molecular events
US7157228B2 (en) * 2002-09-09 2007-01-02 Bioarray Solutions Ltd. Genetic analysis and authentication
US7526114B2 (en) 2002-11-15 2009-04-28 Bioarray Solutions Ltd. Analysis, secure access to, and transmission of array images
US20060068036A1 (en) 2002-12-31 2006-03-30 National Yang-Ming University Extract of Dioscorea sp. and the medical uses thereof
US20040259101A1 (en) * 2003-06-20 2004-12-23 Shuber Anthony P. Methods for disease screening
US7927796B2 (en) 2003-09-18 2011-04-19 Bioarray Solutions, Ltd. Number coding for identification of subtypes of coded types of solid phase carriers
WO2005031305A2 (en) 2003-09-22 2005-04-07 Bioarray Solutions, Ltd. Surface immobilized polyelectrolyte with multiple functional groups capable of covalently bonding to biomolecules
US7563569B2 (en) 2003-10-28 2009-07-21 Michael Seul Optimization of gene expression analysis using immobilized capture probes
JP2007509629A (ja) 2003-10-29 2007-04-19 バイオアレイ ソリューションズ リミテッド 二本鎖ｄｎａの切断による複合核酸分析
WO2005111244A2 (en) * 2004-05-10 2005-11-24 Exact Sciences Corporation Methods for detecting a mutant nucleic acid
WO2005113769A1 (en) * 2004-05-14 2005-12-01 Exact Sciences Corporation Method for stabilizing biological samples for nucleic acid analysis
US7848889B2 (en) 2004-08-02 2010-12-07 Bioarray Solutions, Ltd. Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification
EP1623996A1 (de) * 2004-08-06 2006-02-08 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Verbessertes Verfahren zur Auswahl eines Proteins von einer Bibliothek
WO2006026654A2 (en) 2004-08-27 2006-03-09 Exact Sciences Corporation Method for detecting a recombinant event
WO2006047787A2 (en) 2004-10-27 2006-05-04 Exact Sciences Corporation Method for monitoring disease progression or recurrence
US9777314B2 (en) * 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples
US8486629B2 (en) 2005-06-01 2013-07-16 Bioarray Solutions, Ltd. Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation
JP2009500019A (ja) * 2005-07-01 2009-01-08 プロメガ・コーポレーション 生体分子の精製のための浮遊性粒子のネットワーク、及び生体分子の精製のための浮遊性粒子又は浮遊性粒子のネットワークの使用
US8030034B2 (en) * 2005-12-09 2011-10-04 Promega Corporation Nucleic acid purification with a binding matrix
EP2004849A2 (de) * 2006-03-10 2008-12-24 Council of Scientific & Industrial Research Verfahren zum screening von bakterienisolaten
US8481267B2 (en) * 2009-08-21 2013-07-09 E. I. Du Pont De Nemours And Company Genetic fingerprinting and identification method
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US8222397B2 (en) * 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
JP2014522659A (ja) 2011-07-25 2014-09-08 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 保存生成物および任意生成物の蛍光標識フラグメントを生成するための核酸の増幅方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4851331A (en) * 1986-05-16 1989-07-25 Allied Corporation Method and kit for polynucleotide assay including primer-dependant DNA polymerase
EP0317239A3 (de) * 1987-11-13 1990-01-17 Native Plants Incorporated Verfahren und Vorrichtung zum Nachweis von Restriktionsfragmentlängenpolymorphien
WO1990001065A1 (en) * 1988-07-26 1990-02-08 Genelabs Incorporated Rna and dna amplification techniques
US4879214A (en) * 1988-11-15 1989-11-07 E. I. Du Pont De Nemours And Company Differentiation of nucleic acid segments on the basis of nucleotide differences
WO1990009455A1 (en) * 1989-02-13 1990-08-23 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
US5043272A (en) * 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers

Also Published As

Publication number Publication date
AU7782091A (en) 1991-10-10
AU639565B2 (en) 1993-07-29
DE69105959T2 (de) 1995-05-18
JPH05505528A (ja) 1993-08-19
ATE115634T1 (de) 1994-12-15
NO923544L (no) 1992-09-11
EP0520039A1 (de) 1992-12-30
BR9106151A (pt) 1993-03-09
DK0520039T3 (da) 1995-02-20
IL97503A0 (en) 1992-06-21
JP2818486B2 (ja) 1998-10-30
PL166895B1 (pl) 1995-06-30
PL293188A1 (en) 1992-11-02
FI924049A (fi) 1992-09-10
NO304655B1 (no) 1999-01-25
WO1991014001A2 (en) 1991-09-19
ES2065684T3 (es) 1995-02-16
CA2078132A1 (en) 1991-09-15
IL97503A (en) 1995-05-26
US5126239A (en) 1992-06-30
NZ237396A (en) 1993-08-26
CA2078132C (en) 1999-08-31
EP0520039B1 (de) 1994-12-14
NO923544D0 (no) 1992-09-11
WO1991014001A3 (en) 1991-10-31
ZA911891B (en) 1992-11-25
FI924049A0 (fi) 1992-09-10

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