DE69224231T2 - Verfahren und Zusammensetzungen für Enzymkomplimentationassays unter Verwendung der Omega-Region der Beta-Galactosidase - Google Patents

Verfahren und Zusammensetzungen für Enzymkomplimentationassays unter Verwendung der Omega-Region der Beta-Galactosidase

Info

Publication number
DE69224231T2
DE69224231T2 DE69224231T DE69224231T DE69224231T2 DE 69224231 T2 DE69224231 T2 DE 69224231T2 DE 69224231 T DE69224231 T DE 69224231T DE 69224231 T DE69224231 T DE 69224231T DE 69224231 T2 DE69224231 T2 DE 69224231T2
Authority
DE
Germany
Prior art keywords
beta
galactosidase
omega
compositions
complementation assays
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69224231T
Other languages
English (en)
Other versions
DE69224231D1 (de
Inventor
Mark Krevolin
David Kates
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics Corp
Original Assignee
Boehringer Mannheim Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Corp filed Critical Boehringer Mannheim Corp
Publication of DE69224231D1 publication Critical patent/DE69224231D1/de
Application granted granted Critical
Publication of DE69224231T2 publication Critical patent/DE69224231T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
DE69224231T 1991-05-15 1992-05-14 Verfahren und Zusammensetzungen für Enzymkomplimentationassays unter Verwendung der Omega-Region der Beta-Galactosidase Expired - Fee Related DE69224231T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US70054991A 1991-05-15 1991-05-15

Publications (2)

Publication Number Publication Date
DE69224231D1 DE69224231D1 (de) 1998-03-05
DE69224231T2 true DE69224231T2 (de) 1998-07-23

Family

ID=24813926

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69224231T Expired - Fee Related DE69224231T2 (de) 1991-05-15 1992-05-14 Verfahren und Zusammensetzungen für Enzymkomplimentationassays unter Verwendung der Omega-Region der Beta-Galactosidase

Country Status (8)

Country Link
US (1) US5362625A (de)
EP (1) EP0514173B1 (de)
JP (1) JP2944823B2 (de)
AT (1) ATE162891T1 (de)
AU (1) AU657532B2 (de)
CA (1) CA2068190C (de)
DE (1) DE69224231T2 (de)
ES (1) ES2114548T3 (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5976857A (en) * 1996-01-26 1999-11-02 Boehringer Mannheim Corporation Cross-linked polypeptide fragments of β-galactosidase
US5763196A (en) * 1996-01-26 1998-06-09 Boehringer Mannheim Corporation Assays using cross-linked polypeptide fragments of β-galactosidase
WO1997027203A1 (en) * 1996-01-26 1997-07-31 Boehringer Mannheim Corporation Bis-maleimido cross-linking agents
AU783091B2 (en) * 1997-01-31 2005-09-22 Odyssey Thera, Inc. Protein fragment complementation assays to detect biomolecular interactions
CA2196496A1 (en) 1997-01-31 1998-07-31 Stephen William Watson Michnick Protein fragment complementation assay for the detection of protein-protein interactions
US6342345B1 (en) 1997-04-02 2002-01-29 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by reporter subunit complementation
CA2205888A1 (en) * 1997-07-11 1999-01-11 John J. Priatel Complementation trap
WO1999006537A1 (en) * 1997-07-30 1999-02-11 Boehringer Mannheim Corporation Cross-linked polypeptide assay components
GB2344886B (en) * 1999-03-10 2000-11-01 Medical Res Council Selection of intracellular immunoglobulins
US8148110B2 (en) * 1999-03-15 2012-04-03 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by β-lactamase reporter fragment complementation
US8586294B2 (en) * 2004-05-18 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Detection of protein translocation by beta-galactosidase reporter fragment complementation
AU2006306344A1 (en) * 2005-10-24 2007-05-03 Discoverx Corporation Detection of intracellular enzyme complex
CA2645663C (en) * 2006-03-13 2013-11-05 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions using a reduced affinity enzyme complementation reporter system
US20070254338A1 (en) * 2006-04-24 2007-11-01 Amgen Inc. Method for making recombinant protein using complementation dependent DHFR mutants
US8101373B2 (en) * 2007-10-12 2012-01-24 Discoverx Corporation β-galactosidase donor fragments
EP2665737A1 (de) 2011-01-18 2013-11-27 General Atomics Hydrolaseenzymsubstrate und ihre verwendung
CA2840269C (en) 2011-06-23 2015-08-18 Discoverx Corporation Monitoring protein trafficking using beta-galactosidase reporter fragment complementation
US20150267209A1 (en) * 2012-10-22 2015-09-24 Life Technologies Corporation System and Method for Visualization of Optimized Protein Expression
CN110386986B (zh) * 2018-06-22 2024-01-26 深圳琅技生命科技有限公司 人工改造蛋白质及其构建方法与应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4378428A (en) * 1981-03-30 1983-03-29 Baker Instruments Corporation Method for carrying out non-isotopic immunoassays, labeled analytes and kits for use in such assays
US4708929A (en) * 1984-10-29 1987-11-24 Microgenics Corporation Methods for protein binding enzyme complementation assays
DE3587549T2 (de) * 1984-10-29 1994-02-17 Microgenics Corp Ergänzungstestverfahren von proteine bindenden enzymen.
WO1990013569A1 (en) * 1989-05-05 1990-11-15 Microgenics Corporation Method for protein binding enzyme complementation assays
US5244785A (en) * 1991-02-01 1993-09-14 Microgenics Corporation Determination of high molecular weight analytes using a β-galactosidase complementation assay

Also Published As

Publication number Publication date
JPH05276959A (ja) 1993-10-26
CA2068190C (en) 1996-12-17
JP2944823B2 (ja) 1999-09-06
US5362625A (en) 1994-11-08
CA2068190A1 (en) 1992-11-16
AU657532B2 (en) 1995-03-16
EP0514173A2 (de) 1992-11-19
AU1629192A (en) 1993-03-11
ES2114548T3 (es) 1998-06-01
ATE162891T1 (de) 1998-02-15
DE69224231D1 (de) 1998-03-05
EP0514173A3 (en) 1994-07-06
EP0514173B1 (de) 1998-01-28

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: ROCHE DIAGNOSTICS CORP., INDIANAPOLIS, IND., US

8339 Ceased/non-payment of the annual fee