DE69319596D1 - Molekulargewichtstrennung von biomolekülen durch kapillare elektrophorese unter verwendung einer polymerhaltigen lösung - Google Patents

Molekulargewichtstrennung von biomolekülen durch kapillare elektrophorese unter verwendung einer polymerhaltigen lösung

Info

Publication number
DE69319596D1
DE69319596D1 DE69319596T DE69319596T DE69319596D1 DE 69319596 D1 DE69319596 D1 DE 69319596D1 DE 69319596 T DE69319596 T DE 69319596T DE 69319596 T DE69319596 T DE 69319596T DE 69319596 D1 DE69319596 D1 DE 69319596D1
Authority
DE
Germany
Prior art keywords
molecular weight
polymer
biomolecules
capillary electrophoresis
containing solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69319596T
Other languages
English (en)
Other versions
DE69319596T2 (de
Inventor
David Demorest
William Werner
John Wiktorowicz
H-Michael Wenz
Frank Oaks
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Application granted granted Critical
Publication of DE69319596D1 publication Critical patent/DE69319596D1/de
Publication of DE69319596T2 publication Critical patent/DE69319596T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories
    • G01N27/44752Controlling the zeta potential, e.g. by wall coatings
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories
    • G01N27/44747Composition of gel or of carrier mixture
DE69319596T 1992-05-01 1993-04-30 Molekulargewichtstrennung von biomolekülen durch kapillare elektrophorese unter verwendung einer polymerhaltigen lösung Expired - Lifetime DE69319596T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/877,956 US5264101A (en) 1989-11-06 1992-05-01 Capillary electrophoresis molecular weight separation of biomolecules using a polymer-containing solution
PCT/US1993/004078 WO1993022665A1 (en) 1992-05-01 1993-04-30 Capillary electrophoresis molecular weight separation of biomolecules using a polymer-containing solution

Publications (2)

Publication Number Publication Date
DE69319596D1 true DE69319596D1 (de) 1998-08-13
DE69319596T2 DE69319596T2 (de) 1999-03-04

Family

ID=25371084

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69319596T Expired - Lifetime DE69319596T2 (de) 1992-05-01 1993-04-30 Molekulargewichtstrennung von biomolekülen durch kapillare elektrophorese unter verwendung einer polymerhaltigen lösung

Country Status (6)

Country Link
US (1) US5264101A (de)
EP (1) EP0638168B1 (de)
JP (1) JP3176373B2 (de)
AT (1) ATE168194T1 (de)
DE (1) DE69319596T2 (de)
WO (1) WO1993022665A1 (de)

Families Citing this family (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5332481A (en) 1991-01-29 1994-07-26 Beckman Instruments, Inc. Capillary electrophoresis using replaceable gels
US5384024A (en) * 1992-03-13 1995-01-24 Applied Biosystems, Inc. Capillary electrophoresis
WO1995010771A1 (en) * 1993-10-14 1995-04-20 Bio-Rad Laboratories, Inc. Suppression of electroendosmosis during electrophoresis in gel-free polymer media by use of charged polymers
US5891313A (en) * 1993-11-23 1999-04-06 The Perkin-Elmer Corp. Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks
AU1254295A (en) * 1993-12-17 1995-07-03 Perkin-Elmer Corporation, The Uncharged polymers for separation of biomolecules by capillary electrophoresis
US6358385B1 (en) * 1993-12-17 2002-03-19 The Perkin-Elmer Corporation Polymers for separation of biomolecules by capillary electrophoresis
US5423966A (en) * 1994-01-25 1995-06-13 Perkin-Elmer Corporation On line ion contaminant removal apparatus and method for capillary electrophoresis
US5503722A (en) * 1994-02-28 1996-04-02 Beckman Instruments, Inc. Rehydratable gels for capillary electrophoresis
DE69633962T2 (de) * 1995-01-27 2005-12-01 Northeastern University, Boston Verfahren zum Bilden einer kovalent gebundenen hydrophilen Schicht auf Basis von Polyvinyl-Alkohol für die Kapillarelektrophorese
US5582705A (en) * 1995-05-19 1996-12-10 Iowa State University Research Foundation, Inc. Multiplexed capillary electrophoresis system
AU1375097A (en) * 1995-12-28 1997-07-28 Novartis Ag Capillary electrophoretic separation method using a fillable polymer solution as separating agent
US5942443A (en) 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
US6001232A (en) * 1996-09-04 1999-12-14 The Research Foundation Of State University Of New York Separation medium for capillary electrophoresis
US5989399A (en) * 1996-09-04 1999-11-23 The Research Foundation Of State University Of New York Effective surface treatment for a new separation medium in electrophoresis
US5964995A (en) * 1997-04-04 1999-10-12 Caliper Technologies Corp. Methods and systems for enhanced fluid transport
US5976336A (en) 1997-04-25 1999-11-02 Caliper Technologies Corp. Microfluidic devices incorporating improved channel geometries
US7033474B1 (en) 1997-04-25 2006-04-25 Caliper Life Sciences, Inc. Microfluidic devices incorporating improved channel geometries
AU727083B2 (en) * 1997-04-25 2000-11-30 Caliper Life Sciences, Inc. Microfluidic devices incorporating improved channel geometries
US5958694A (en) 1997-10-16 1999-09-28 Caliper Technologies Corp. Apparatus and methods for sequencing nucleic acids in microfluidic systems
US5948227A (en) * 1997-12-17 1999-09-07 Caliper Technologies Corp. Methods and systems for performing electrophoretic molecular separations
US6013165A (en) * 1998-05-22 2000-01-11 Lynx Therapeutics, Inc. Electrophoresis apparatus and method
EP1084391A4 (de) 1998-06-08 2006-06-14 Caliper Life Sciences Inc Mikrofluidische geräte, systeme und verfahren zur durchführung integrierter reaktionen und trennungen
US6706161B1 (en) * 1998-06-10 2004-03-16 Agilent Technologies, Inc. Methods and compositions for modifying the surface of a polymeric substrate
US6800287B2 (en) * 1998-09-25 2004-10-05 Yeda Research And Development Co., Ltd. Copolymer 1 related polypeptides for use as molecular weight markers and for therapeutic use
US6475364B1 (en) 1999-02-02 2002-11-05 Caliper Technologies Corp. Methods, devices and systems for characterizing proteins
US6599836B1 (en) * 1999-04-09 2003-07-29 Micron Technology, Inc. Planarizing solutions, planarizing machines and methods for mechanical or chemical-mechanical planarization of microelectronic-device substrate assemblies
US6407000B1 (en) * 1999-04-09 2002-06-18 Micron Technology, Inc. Method and apparatuses for making and using bi-modal abrasive slurries for mechanical and chemical-mechanical planarization of microelectronic-device substrate assemblies
US6306273B1 (en) * 1999-04-13 2001-10-23 Aclara Biosciences, Inc. Methods and compositions for conducting processes in microfluidic devices
US6716948B1 (en) * 1999-07-31 2004-04-06 Symyx Technologies, Inc. Controlled-architecture polymers and use thereof as separation media
US6680208B1 (en) * 1999-11-19 2004-01-20 Becton, Dickinson And Company Rapid protein identification using antibody mixtures
US20020012971A1 (en) * 2000-03-20 2002-01-31 Mehta Tammy Burd PCR compatible nucleic acid sieving medium
EP1285260A4 (de) * 2000-04-25 2006-02-01 Spectrumedix Llc Denaturiermittelfreie elektrophorese biologischer moleküle bei hohen temperaturen
FR2810905B1 (fr) * 2000-06-30 2003-05-16 Inst Curie Additif pour minimiser les phenomenes d'adsorption et/ou d'electroosmose
US6706162B1 (en) * 2000-09-25 2004-03-16 Applera Corporation High speed, high resolution compositions, methods, and kits for capillary electrophoresis
US20030085127A1 (en) * 2001-06-13 2003-05-08 Symyx Technologies, Inc. Polymer additive useful in separation media
JP4657524B2 (ja) * 2001-08-29 2011-03-23 嘉信 馬場 マイクロチップ電気泳動用装置及びそれを用いる電気泳動方法
WO2003062810A1 (en) * 2002-01-22 2003-07-31 Board Of Regents, The University Of Texas System Electrophoretic ratchets and cyclic electrophoresis
US20040029143A1 (en) * 2002-02-28 2004-02-12 Jeffrey Van Ness Cationic polyelectrolytes in biomolecule purification and analysis
DE10243930A1 (de) * 2002-03-08 2003-09-25 Conducta Endress & Hauser Vorrichtung zur Messung und Regelung des pH-Wertes eines Mediums
US7381317B2 (en) * 2002-08-12 2008-06-03 Beckman Coulter, Inc. Methods and compositions for capillary electrophoresis (CE)
US7083711B2 (en) * 2003-02-21 2006-08-01 Combisep, Inc. Capillary electrophoresis gel especially for separation made for single stranded nucleic acid separations
US20050025741A1 (en) 2003-05-15 2005-02-03 Lau Aldrich N.K. Poly and copoly(N-vinylamide)s and their use in capillary electrophoresis
WO2004106888A2 (en) * 2003-05-22 2004-12-09 Spectrumedix Llc Electrophoresis capillaries having reduced amounts of -oh
JP4450368B2 (ja) 2004-03-04 2010-04-14 独立行政法人産業技術総合研究所 マイクロ流路チップの製造方法、マイクロ流路チップ、そのマイクロ流路チップを用いる生体分子の分離方法、およびそのマイクロ流路チップを有する電気泳動装置
US7846676B2 (en) 2004-07-19 2010-12-07 Cell Biosciences, Inc. Methods and devices for analyte detection
US7935479B2 (en) * 2004-07-19 2011-05-03 Cell Biosciences, Inc. Methods and devices for analyte detection
KR100647306B1 (ko) * 2004-12-23 2006-11-23 삼성전자주식회사 아미노기와 카르복실기를 포함하고 제1 pH에서 양전하를띠는 물질을 이용하여 핵산을 분리하는 방법
JP4645255B2 (ja) * 2005-03-24 2011-03-09 株式会社島津製作所 分析試料調製用溶媒
EP1919618A2 (de) * 2005-05-21 2008-05-14 Core-Microsolutions, Inc. Minderung von biomoleküladsorption mit hydrophilen polymeradditiven
US20070014699A1 (en) * 2005-06-23 2007-01-18 Beckman Coulter, Inc, Methods and apparatus for improving the sensitivity of capillary zone electrophoresis
AU2005335179B2 (en) * 2005-08-01 2011-03-17 Nard Institute, Ltd. Polyacrylamide gel for electrophoresis, polyacrylamide gel electrophoresis method using the same, method of producing the same and acrylamide compound
WO2007035864A2 (en) 2005-09-20 2007-03-29 Cell Biosciences, Inc. Electrophoresis standards, methods and kits
US10107782B2 (en) 2008-01-25 2018-10-23 ProteinSimple Method to perform limited two dimensional separation of proteins and other biologicals
US9841401B2 (en) 2009-04-14 2017-12-12 National Cheng Kung University Capillary electrophoresis method for analyzing collagen
US20100258439A1 (en) * 2009-04-14 2010-10-14 Huang Lynn Ling-Huei Capillary electrophoresis method for analyzing collagen and an assay kit for the same
JP5917567B2 (ja) 2011-01-31 2016-05-18 キアゲン マンスフィールド インコーポレイテッド アーチファクトが減少した、核酸の変性キャピラリー電気泳動
EP2839276B1 (de) 2012-04-19 2018-06-27 ProteinSimple Isoelektrische fokussierung mit zwei wellenlängen zur bestimmung der wirkstofflast in antikörper-wirkstoff-konjugaten
JP6348258B2 (ja) * 2013-06-20 2018-06-27 大塚電子株式会社 分離分析方法
US9766206B2 (en) 2013-09-27 2017-09-19 ProteinSimple Apparatus, systems, and methods for capillary electrophoresis
WO2018051374A1 (en) * 2016-09-15 2018-03-22 Dianax S.R.L. Microfluidic device, particularly of the lab-on-chip type, for the concentration, purification, and detection of biological and/or medical targets of interest from a biological sample
US11946900B2 (en) * 2018-04-09 2024-04-02 Shimadzu Corporation Electrophoresis flow channel cleaning method and electrophoresis device
EP3757176B1 (de) * 2019-06-27 2022-08-31 Canon Production Printing Holding B.V. Tintenstrahlbeschichtungszusammensetzung
US10985617B1 (en) 2019-12-31 2021-04-20 Energous Corporation System for wirelessly transmitting energy at a near-field distance without using beam-forming control
WO2023164258A1 (en) * 2022-02-28 2023-08-31 Perkinelmer Health Sciences, Inc. Methods for separating and detecting double-stranded and single-stranded ribonucleic acid (rna)

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4405798A (en) * 1981-03-30 1983-09-20 Celanese Corporation Acrylate and methacrylate monoesters of pentaerythritol and pentaerythritol orthoesters and polymers and copolymers derived therefrom
JPS6060548A (ja) * 1983-09-14 1985-04-08 Fuji Photo Film Co Ltd 電気泳動用媒体
JPS6060549A (ja) * 1983-09-14 1985-04-08 Fuji Photo Film Co Ltd ゲル電気泳動用媒体
US4690749A (en) * 1985-12-16 1987-09-01 Universities Space Research Association Polymer-coated surfaces to control surface zeta potential
US4997537A (en) * 1986-10-21 1991-03-05 Northeastern University High performance microcapillary gel electrophoresis
US4931328A (en) * 1988-08-19 1990-06-05 Hewlett-Packard Company Capillary tube with reduced protein interactions and controllable electroosmotic flow
EP0417925A3 (en) * 1989-09-12 1991-09-11 Northeastern University High performance microcapillary gel electrophoresis
DE69007773T2 (de) * 1990-02-13 1994-09-22 Bio Rad Laboratories Elektrophoretische Trennung in einem gelfreien Medium mittels gelösten Polymeren.
US5069766A (en) * 1990-12-20 1991-12-03 Bio-Rad Laboratories, Inc. Suppression of electroendosmosis in capillary electrophoresis

Also Published As

Publication number Publication date
EP0638168A4 (de) 1995-07-19
JP3176373B2 (ja) 2001-06-18
DE69319596T2 (de) 1999-03-04
JPH07506432A (ja) 1995-07-13
ATE168194T1 (de) 1998-07-15
US5264101A (en) 1993-11-23
WO1993022665A1 (en) 1993-11-11
EP0638168B1 (de) 1998-07-08
EP0638168A1 (de) 1995-02-15

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: APPLERA CORP., FOSTER CITY, CALIF., US

8327 Change in the person/name/address of the patent owner

Owner name: APPLIED BIOSYSTEMS, LLC (N. D. GES. D. STAATES, US

8380 Miscellaneous part iii

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8380 Miscellaneous part iii

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