DE69329141D1 - Verfahren zur Spaltung eines Nucleinsäuremoleküls - Google Patents

Verfahren zur Spaltung eines Nucleinsäuremoleküls

Info

Publication number
DE69329141D1
DE69329141D1 DE69329141T DE69329141T DE69329141D1 DE 69329141 D1 DE69329141 D1 DE 69329141D1 DE 69329141 T DE69329141 T DE 69329141T DE 69329141 T DE69329141 T DE 69329141T DE 69329141 D1 DE69329141 D1 DE 69329141D1
Authority
DE
Germany
Prior art keywords
nucleic acid
cleavage
region
target nucleic
annealed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69329141T
Other languages
English (en)
Other versions
DE69329141T2 (de
Inventor
James E Dahlberg
Victor I Lyamichev
Mary Ann D Brow
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wisconsin Alumni Research Foundation
Original Assignee
Wisconsin Alumni Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Research Foundation filed Critical Wisconsin Alumni Research Foundation
Publication of DE69329141D1 publication Critical patent/DE69329141D1/de
Application granted granted Critical
Publication of DE69329141T2 publication Critical patent/DE69329141T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4746—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used p53
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69329141T 1992-12-07 1993-12-07 Verfahren zur Spaltung eines Nucleinsäuremoleküls Expired - Lifetime DE69329141T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/986,330 US5422253A (en) 1992-12-07 1992-12-07 Method of site specific nucleic acid cleavage

Publications (2)

Publication Number Publication Date
DE69329141D1 true DE69329141D1 (de) 2000-09-07
DE69329141T2 DE69329141T2 (de) 2001-01-11

Family

ID=25532311

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69329141T Expired - Lifetime DE69329141T2 (de) 1992-12-07 1993-12-07 Verfahren zur Spaltung eines Nucleinsäuremoleküls

Country Status (9)

Country Link
US (2) US5422253A (de)
EP (1) EP0601834B1 (de)
JP (1) JP3518884B2 (de)
AT (1) ATE195141T1 (de)
DE (1) DE69329141T2 (de)
DK (1) DK0601834T3 (de)
ES (1) ES2148208T3 (de)
GR (1) GR3034654T3 (de)
PT (1) PT601834E (de)

Families Citing this family (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994069A (en) 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US6872816B1 (en) * 1996-01-24 2005-03-29 Third Wave Technologies, Inc. Nucleic acid detection kits
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5541311A (en) * 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
US5614402A (en) * 1992-12-07 1997-03-25 Third Wave Technologies, Inc. 5' nucleases derived from thermostable DNA polymerase
US5888780A (en) * 1992-12-07 1999-03-30 Third Wave Technologies, Inc. Rapid detection and identification of nucleic acid variants
US6372424B1 (en) * 1995-08-30 2002-04-16 Third Wave Technologies, Inc Rapid detection and identification of pathogens
US6673616B1 (en) 1992-12-07 2004-01-06 Third Wave Technologies, Inc. Methods and compositions for characterizing nucleic acids
US5843654A (en) * 1992-12-07 1998-12-01 Third Wave Technologies, Inc. Rapid detection of mutations in the p53 gene
US7785852B2 (en) 1992-12-07 2010-08-31 Third Wave Technologies, Inc. Cleavage of nucleic acids
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
US20110097791A1 (en) * 1999-04-16 2011-04-28 Engelhardt Dean L Novel process, construct and conjugate for producing multiple nucleic acid copies
US20050123926A1 (en) * 1994-01-13 2005-06-09 Enzo Diagnostics, Inc., In vitro process for producing multiple nucleic acid copies
US6986985B1 (en) 1994-01-13 2006-01-17 Enzo Life Sciences, Inc. Process for producing multiple nucleic acid copies in vivo using a protein-nucleic acid construct
ES2165402T3 (es) * 1994-07-16 2002-03-16 Roche Diagnostics Gmbh Procedimiento para la deteccion sensible de acidos nucleicos.
CA2203627C (en) * 1994-11-09 2000-06-06 James E. Dahlberg Rapid detection and identification of nucleic acid variants and pathogens
EP0842294B1 (de) * 1994-12-23 2002-10-23 Dade Behring Inc. Nachweis von nukleinsäuren durch nuklease-katalysierte produktbildung
US5877280A (en) * 1995-06-06 1999-03-02 The Mount Sinai School Of Medicine Of The City University Of New York Thermostable muts proteins
WO1997015691A1 (en) 1995-10-27 1997-05-01 Ramberg Elliot R Methods and compositions for detection of specific nucleotide sequences
US6875572B2 (en) 1996-01-24 2005-04-05 Third Wave Technologies, Inc. Nucleic acid detection assays
US6706471B1 (en) 1996-01-24 2004-03-16 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US7195871B2 (en) * 1996-01-24 2007-03-27 Third Wave Technologies, Inc Methods and compositions for detecting target sequences
US6913881B1 (en) 1996-01-24 2005-07-05 Third Wave Technologies, Inc. Methods and compositions for detecting target sequences
US7432048B2 (en) * 1996-11-29 2008-10-07 Third Wave Technologies, Inc. Reactions on a solid surface
US20080220425A1 (en) * 1996-01-24 2008-09-11 Third Wave Technologies, Inc. Methods and Compositions for Detecting Target Sequences
US7122364B1 (en) 1998-03-24 2006-10-17 Third Wave Technologies, Inc. FEN endonucleases
US7527928B2 (en) * 1996-11-29 2009-05-05 Third Wave Technologies, Inc. Reactions on a solid surface
EP1634890B1 (de) * 1996-01-24 2008-11-19 Third Wave Technologies, Inc. Invasive Spaltung von Nukleinsäuren
US6090606A (en) * 1996-01-24 2000-07-18 Third Wave Technologies, Inc. Cleavage agents
US5985557A (en) 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US20080160524A1 (en) * 1996-01-24 2008-07-03 Third Wave Technologies, Inc. Methods and Compositions for Detecting Target Sequences
US6140053A (en) * 1996-11-06 2000-10-31 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
CA2273204C (en) 1996-11-29 2008-10-14 Third Wave Technologies, Inc. Fen-1 endonucleases, mixtures and cleavage methods
US6194149B1 (en) 1998-03-03 2001-02-27 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides
US6210880B1 (en) 1997-09-19 2001-04-03 Third Wave Technologies, Inc. Polymorphism analysis by nucleic acid structure probing with structure-bridging oligonucleotides
US7101672B2 (en) 1998-05-05 2006-09-05 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides
EP0983292A4 (de) 1997-05-05 2005-05-18 Third Wave Tech Inc Zielabhängige reaktionen mittels strukturüberbrückender oligonukleotide
US8182991B1 (en) 1997-11-26 2012-05-22 Third Wave Technologies, Inc. FEN-1 endonucleases, mixtures and cleavage methods
US6183958B1 (en) * 1998-05-06 2001-02-06 Variagenics, Inc. Probes for variance detection
WO2000032823A1 (en) * 1998-12-02 2000-06-08 Phylos, Inc. Dna-protein fusions and uses thereof
US6964847B1 (en) 1999-07-14 2005-11-15 Packard Biosciences Company Derivative nucleic acids and uses thereof
US20030207297A1 (en) * 1999-10-13 2003-11-06 Hubert Koster Methods for generating databases and databases for identifying polymorphic genetic markers
EP2088209B1 (de) 1999-10-13 2017-05-31 Sequenom, Inc. Verfahren zur Erzeugung von Datenbanken zur Identifizierung von polymorphen genetischen Markern
US7332275B2 (en) * 1999-10-13 2008-02-19 Sequenom, Inc. Methods for detecting methylated nucleotides
US7118860B2 (en) 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
US7824859B2 (en) * 1999-10-29 2010-11-02 Cytyc Corporation Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase
US6893819B1 (en) * 2000-11-21 2005-05-17 Stratagene California Methods for detection of a nucleic acid by sequential amplification
US7838225B2 (en) * 1999-10-29 2010-11-23 Hologic, Inc. Methods for detection of a target nucleic acid by forming a cleavage structure using a reverse transcriptase
WO2001037878A2 (en) * 1999-11-29 2001-05-31 Orchid Biosciences, Inc. Methods of identifying optimal drug combinations and compositions thereof
US8288322B2 (en) 2000-04-17 2012-10-16 Dyax Corp. Methods of constructing libraries comprising displayed and/or expressed members of a diverse family of peptides, polypeptides or proteins and the novel libraries
JP5149476B2 (ja) * 2000-04-17 2013-02-20 ダイアックス コーポレイション ペプチドの多種多様なファミリーのメンバーとしての、遺伝的パッケージの提示ライブラリーを構築する方法
US20090075256A1 (en) * 2000-06-17 2009-03-19 Third Wave Technologies, Inc. Nucleic Acid Accessible Hybridization Site Identification Using Mass Spectrometry
US7060436B2 (en) * 2000-06-17 2006-06-13 Third Wave Technologies, Inc. Nucleic acid accessible hybridization sites
EP1341929A2 (de) * 2000-11-15 2003-09-10 Third Wave Technologies, Inc. Fen endonukleasen
US7678541B2 (en) * 2000-11-21 2010-03-16 Hologic, Inc. Methods and compositions for the detection of a nucleic acid using a non-invasive cleavage reaction
AU2013205033B2 (en) * 2001-04-17 2016-06-09 Takeda Pharmaceutical Company Limited Novel Methods of Constructing Libraries Comprising Displayed and/or Expressed Members of a Diverse Family of Peptides, Polypeptides or Proteins and the Novel Libraries
AU2003208959A1 (en) * 2002-01-30 2003-09-02 Id Biomedical Corporation Methods for detecting vancomycin-resistant microorganisms and compositions therefor
WO2003093296A2 (en) * 2002-05-03 2003-11-13 Sequenom, Inc. Kinase anchor protein muteins, peptides thereof, and related methods
CA2507189C (en) 2002-11-27 2018-06-12 Sequenom, Inc. Fragmentation-based methods and systems for sequence variation detection and discovery
WO2004061131A1 (en) * 2002-12-18 2004-07-22 Aclara Biosciences, Inc. Multiplexed immunohistochemical assays using molecular tags
US20070141570A1 (en) * 2003-03-07 2007-06-21 Sequenom, Inc. Association of polymorphic kinase anchor proteins with cardiac phenotypes and related methods
US20040214176A1 (en) * 2003-04-22 2004-10-28 Osborne James C. Multiplexed DNA assays using structure-specific endonucleases
US9394565B2 (en) 2003-09-05 2016-07-19 Agena Bioscience, Inc. Allele-specific sequence variation analysis
WO2005098050A2 (en) * 2004-03-26 2005-10-20 Sequenom, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
US7608394B2 (en) 2004-03-26 2009-10-27 Sequenom, Inc. Methods and compositions for phenotype identification based on nucleic acid methylation
US20060029954A1 (en) * 2004-06-30 2006-02-09 Applera Corporation Compositions and methods for identifying nucleotides in polynucleotide sequences
KR100652903B1 (ko) * 2005-12-21 2006-12-04 한국과학기술연구원 초흡수성 고분자를 함유한 제습제의 제조 방법 및 그 제조장치
EP2021506B1 (de) * 2006-04-28 2016-03-16 Igor Kutyavin Verwendung von pcr-amplifikationsprodukten mit elementen sekundärer struktur für verbesserten pcr-basierten nukleinsäurenachweis
US9873957B2 (en) 2008-03-13 2018-01-23 Dyax Corp. Libraries of genetic packages comprising novel HC CDR3 designs
KR101419980B1 (ko) * 2008-03-15 2014-07-15 홀로직, 인크. 증폭 반응 도중에 핵산 분자를 분석하기 위한 조성물 및 방법
EP2281078B1 (de) 2008-04-24 2014-10-22 Dyax Corporation Bibliotheken von genetischen packungen, umfassend neue hc-cdr1, -cdr2, und -cdr3, und neue lc-cdr1-, -cdr2- und -cdr3-designs
US20110207132A1 (en) * 2008-08-04 2011-08-25 University Of Maryland Probes and methods for detecting analytes
US9914963B2 (en) 2009-09-28 2018-03-13 Igor Kutyavin Methods and compositions for detection of nucleic acids based on stabilized oligonucleotide probe complexes
BR112013021144A2 (pt) * 2011-02-18 2016-11-22 Nvs Technologies Inc detecção quantitativa altamente multiplexada de ácidos nucléicos
EP2780479A4 (de) * 2011-11-17 2015-04-08 Nvs Technologies Inc Quantitativer hochmultiplexierter nachweis von nukleinsäuren
WO2013187628A1 (en) * 2012-06-11 2013-12-19 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent transcription
CN105247076B (zh) * 2013-03-15 2021-06-18 莱尔·J·阿诺德 使用拼装序列扩增片段化的目标核酸的方法
WO2020028729A1 (en) * 2018-08-01 2020-02-06 Mammoth Biosciences, Inc. Programmable nuclease compositions and methods of use thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935357A (en) * 1986-02-05 1990-06-19 New England Biolabs, Inc. Universal restriction endonuclease
CA1340323C (en) * 1988-09-20 1999-01-19 Arnold E. Hampel Rna catalyst for cleaving specific rna sequences
DD299440A5 (de) * 1989-06-29 1992-04-16 Krueger,Dieter,De Verfahren zur vollstaendigen restriktion von dna-molekuelen
ES2121777T3 (es) * 1989-12-22 1998-12-16 Hoffmann La Roche Vectores de expresion recombinantes y metodos de purificacion de la dna polimerasa de thermus thermophilus.
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
EP0894860B1 (de) * 1990-09-28 2012-12-12 F. Hoffmann-La Roche AG Thermostabile DNA Polymerasen und deren Deletionsmutanten

Also Published As

Publication number Publication date
PT601834E (pt) 2001-01-31
ATE195141T1 (de) 2000-08-15
ES2148208T3 (es) 2000-10-16
JP3518884B2 (ja) 2004-04-12
US5422253A (en) 1995-06-06
DK0601834T3 (da) 2000-09-04
GR3034654T3 (en) 2001-01-31
US5871911A (en) 1999-02-16
JPH06303975A (ja) 1994-11-01
EP0601834B1 (de) 2000-08-02
EP0601834A1 (de) 1994-06-15
DE69329141T2 (de) 2001-01-11

Similar Documents

Publication Publication Date Title
DE69329141D1 (de) Verfahren zur Spaltung eines Nucleinsäuremoleküls
DE3751923D1 (de) Verfahren zur Klonierung von Restriktions-Modifikationssystemen
KR860005772A (ko) 하이드로포르밀화 공정을 이용한 알데하이드의 제조방법
ATA44185A (de) Verfahren zur herstellung von nucleinsaeure-reagenzien
DE69325389D1 (de) Verfahren zur stabile transformation von weizen
DE69433010D1 (de) Verfahren zur darstellung von oligonukleotiden
ATE85088T1 (de) Verfahren der beschleunigten wiederassoziation der nukleinsaeure.
DE3773791D1 (de) Verfahren zur partialoxydation eines kohlenwasserstoffbrennstoffs.
DE3579430D1 (de) Verfahren zur gewinnung von methacrylsaeure.
DE69403210T2 (de) Verfahren zur herstellung von n-acetylneuraminsäure
ATE55403T1 (de) Verfahren zur spaltung von kohlenwasserstoffeinsaetzen.
DE3686132D1 (de) Verfahren zur herstellung von selbstalignierten halbleiterstrukturen.
DE3677042D1 (de) Verfahren zur herstellung von 2-amino-3-nitro-6-(4-fluor-benzyl-amino)-pyridin sowie 2-amino-3-carbethoxyamino-6-(4-fluor-benzyl-amino)-pyridin.
ATE194329T1 (de) Verfahren zur stereospezifischen hydrolyse von piperidin-dion derivaten
DE59007810D1 (de) Verfahren zur enzymatischen Spaltung von 2-Arylpropionsäurevinylestern.
DE69006489D1 (de) Verfahren zur kontinuierlichen wiedergewinnung von fluorwasserstoffgas.
DE3874565D1 (de) Verfahren zur herstellung von hydroxybiphenylen.
DE3677838D1 (de) Verfahren zur herstellung von alkylestern von 3,4-epoxybuttersaeure.
DE3575163D1 (de) Verfahren zur kultivierung transformierter hefe.
DE3866135D1 (de) Verfahren zur herstellung von mineraldispersionen.
DE3574966D1 (de) Verfahren zur herstellung von 1,2-aminoalkoholen.
ATE4782T1 (de) Verfahren zur herstellung von maenteln fuer auspufftoepfe mit einer gefalzten laengsnaht und vorrichtung zur durchfuehrung dieses verfahrens.
DE3765575D1 (de) Verfahren zur herstellung von bromierten bisphenolen.
AT369758B (de) Verfahren zur herstellung von alkenungesaettigten oligourethanen
BR8402084A (pt) Processo e sistema de correcao de perfis de produtividade e/ou injetividade de pocos de petroleo

Legal Events

Date Code Title Description
8364 No opposition during term of opposition