DE69333671D1 - Verfahren zur herstellung von polypeptide durch bakterielle fermentation - Google Patents

Verfahren zur herstellung von polypeptide durch bakterielle fermentation

Info

Publication number
DE69333671D1
DE69333671D1 DE69333671T DE69333671T DE69333671D1 DE 69333671 D1 DE69333671 D1 DE 69333671D1 DE 69333671 T DE69333671 T DE 69333671T DE 69333671 T DE69333671 T DE 69333671T DE 69333671 D1 DE69333671 D1 DE 69333671D1
Authority
DE
Germany
Prior art keywords
bacterial fermentation
producing polypeptides
polypeptides
producing
fermentation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69333671T
Other languages
English (en)
Other versions
DE69333671T2 (de
Inventor
R Swartz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of DE69333671D1 publication Critical patent/DE69333671D1/de
Application granted granted Critical
Publication of DE69333671T2 publication Critical patent/DE69333671T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/20—Mixing gases with liquids
    • B01F23/23—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids
    • B01F23/233—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using driven stirrers with completely immersed stirring elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/20—Mixing gases with liquids
    • B01F23/23—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids
    • B01F23/233—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using driven stirrers with completely immersed stirring elements
    • B01F23/2331—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using driven stirrers with completely immersed stirring elements characterised by the introduction of the gas along the axis of the stirrer or along the stirrer elements
    • B01F23/23316—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using driven stirrers with completely immersed stirring elements characterised by the introduction of the gas along the axis of the stirrer or along the stirrer elements through a separate hollow guide substantially parallel with the stirrer element
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/20—Mixing gases with liquids
    • B01F23/23—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids
    • B01F23/237—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids characterised by the physical or chemical properties of gases or vapours introduced in the liquid media
    • B01F23/2376—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids characterised by the physical or chemical properties of gases or vapours introduced in the liquid media characterised by the gas being introduced
    • B01F23/23761—Aerating, i.e. introducing oxygen containing gas in liquids
    • B01F23/237612—Oxygen
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/20—Measuring; Control or regulation
    • B01F35/21—Measuring
    • B01F35/213—Measuring of the properties of the mixtures, e.g. temperature, density or colour
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/20—Measuring; Control or regulation
    • B01F35/21—Measuring
    • B01F35/2132—Concentration, pH, pOH, p(ION) or oxygen-demand
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/20—Measuring; Control or regulation
    • B01F35/22—Control or regulation
    • B01F35/221—Control or regulation of operational parameters, e.g. level of material in the mixer, temperature or pressure
    • B01F35/2211—Amount of delivered fluid during a period
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors (Somatomedins), e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/28—Means for regulation, monitoring, measurement or control, e.g. flow regulation of redox potential
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0053—Oxidoreductases (1.) acting on a heme group of donors (1.9)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/20—Mixing gases with liquids
    • B01F23/23—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids
    • B01F23/233—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using driven stirrers with completely immersed stirring elements
    • B01F23/2336—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using driven stirrers with completely immersed stirring elements characterised by the location of the place of introduction of the gas relative to the stirrer
    • B01F23/23362—Mixing gases with liquids by introducing gases into liquid media, e.g. for producing aerated liquids using driven stirrers with completely immersed stirring elements characterised by the location of the place of introduction of the gas relative to the stirrer the gas being introduced under the stirrer
DE69333671T 1992-11-23 1993-11-19 Verfahren zur herstellung von polypeptide durch bakterielle fermentation Expired - Lifetime DE69333671T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US989844 1992-11-23
US07/989,844 US5342763A (en) 1992-11-23 1992-11-23 Method for producing polypeptide via bacterial fermentation
PCT/US1993/011297 WO1994012630A2 (en) 1992-11-23 1993-11-19 Method for producing polypeptide via bacterial fermentation

Publications (2)

Publication Number Publication Date
DE69333671D1 true DE69333671D1 (de) 2004-11-25
DE69333671T2 DE69333671T2 (de) 2006-01-19

Family

ID=25535522

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69334286T Expired - Lifetime DE69334286D1 (de) 1992-11-23 1993-11-19 Verfahren zur Bestimmung der Neigung von Bakterien zum gelöstem Sauerstofinstabilität
DE69333671T Expired - Lifetime DE69333671T2 (de) 1992-11-23 1993-11-19 Verfahren zur herstellung von polypeptide durch bakterielle fermentation

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE69334286T Expired - Lifetime DE69334286D1 (de) 1992-11-23 1993-11-19 Verfahren zur Bestimmung der Neigung von Bakterien zum gelöstem Sauerstofinstabilität

Country Status (12)

Country Link
US (3) US5342763A (de)
EP (2) EP1524316B1 (de)
JP (2) JP3513875B2 (de)
AT (2) ATE432344T1 (de)
AU (2) AU669414B2 (de)
CA (1) CA2147468C (de)
DE (2) DE69334286D1 (de)
DK (2) DK0668906T3 (de)
ES (2) ES2326802T3 (de)
IL (1) IL107712A (de)
PT (2) PT668906E (de)
WO (1) WO1994012630A2 (de)

Families Citing this family (31)

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US5342763A (en) * 1992-11-23 1994-08-30 Genentech, Inc. Method for producing polypeptide via bacterial fermentation
US6274369B1 (en) * 1996-02-02 2001-08-14 Invitrogen Corporation Method capable of increasing competency of bacterial cell transformation
AU2660397A (en) * 1996-04-05 1997-10-29 Board Of Regents, The University Of Texas System Methods for producing soluble, biologically-active disulfide bond-containing eukaryotic proteins in bacterial cells
US6114137A (en) * 1996-10-31 2000-09-05 Genentech Inc Assay using marked microbial host cell strains
ZA979496B (en) * 1996-10-31 1999-04-23 Genentech Inc Assay using marked microbial host cell strains
JP2001503274A (ja) * 1996-11-08 2001-03-13 サイテル コーポレイション 改良された発現ベクター
US6083715A (en) * 1997-06-09 2000-07-04 Board Of Regents, The University Of Texas System Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells
IL143866A0 (en) * 1999-01-06 2002-04-21 Genentech Inc Insulin-like growth factor (igf) i mutant variants
IL143834A0 (en) * 1999-01-06 2002-04-21 Genentech Inc Insulin-like growth factor (igf) i mutant variants
AU2001263215A1 (en) 2000-05-16 2001-11-26 Genentech Inc. Method for treating cartilage disorders
SE0003958D0 (sv) 2000-10-31 2000-10-31 Biogaia Fermentation Ab Method for growth of microorganisms
PT1332222E (pt) 2000-11-03 2009-06-30 Genentech Inc Desvios de taxas metabólicas em fermentações expressando proteínas recombinantes
US7071300B2 (en) * 2001-03-14 2006-07-04 Genentech, Inc. IGF antagonist peptides
WO2004065574A2 (en) * 2003-01-16 2004-08-05 Bloom Fredric R Bacteria for high efficiency cloning
US7249880B2 (en) 2003-10-14 2007-07-31 Advanced Technology Materials, Inc. Flexible mixing bag for mixing solids, liquids and gases
CA2946323A1 (en) * 2004-03-11 2005-09-22 Genentech, Inc. Process for producing polypeptides
US7078201B2 (en) * 2004-12-01 2006-07-18 Burmaster Brian M Ethanol fermentation using oxidation reduction potential
AU2005318999B2 (en) 2004-12-22 2012-07-19 Genentech, Inc. Methods for producing soluble multi-membrane-spanning proteins
EP1877636B1 (de) * 2005-04-15 2012-12-26 BROWN, Garrett W. Ausgleichsstützvorrichtung
JP4358785B2 (ja) * 2005-05-27 2009-11-04 麒麟麦酒株式会社 密封容器内の酸素量の測定方法及びこれに用いる密封容器のピアス装置
JP2009513140A (ja) * 2005-10-26 2009-04-02 リーブテック，インコーポレイテッド ミキサーとスパージャーを備えたバイオリアクター
BRPI0621124A2 (pt) 2005-12-22 2011-11-29 Genentech Inc métodos para recuperação de uma proteìna de ligação à heparina
DE102006001623B4 (de) * 2006-01-11 2009-05-07 Sartorius Stedim Biotech Gmbh Behälter und Verfahren zum Mischen von Medien
US8366311B2 (en) 2006-04-21 2013-02-05 Atmi Bvba Systems and devices for mixing substances and methods of making same
WO2007134267A2 (en) * 2006-05-13 2007-11-22 Advanced Technology Materials, Inc. Disposable bioreactor
JP2009072133A (ja) * 2007-09-21 2009-04-09 Hitachi Plant Technologies Ltd 培養槽装置、細胞培養方法及び物質生産方法
EP2216395A1 (de) 2009-02-09 2010-08-11 Lonza Biologics plc. Bioreaktor zur Kultivierung von Säugetierzellen
HUE025488T2 (en) 2009-06-05 2016-04-28 Hoffmann La Roche Equipment for cell culture
BRPI1010068A2 (pt) * 2009-07-24 2016-03-15 Hoffmann La Roche "sistema agitador, dispositivo, método para cultivo de células animais, método para produzir um polipeptídeo, uso de um sistema agitador e dispositivos para cultivar células animais"
US20140024090A1 (en) * 2011-12-19 2014-01-23 Shell Oil Company Method and apparatus for converting a carbon source into a hydrocarbon or hydrocarbon precursor
CA3159562A1 (en) * 2019-10-30 2021-05-06 Genomatica, Inc. Microorganisms and methods for reducing by-products

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US3384553A (en) * 1965-04-08 1968-05-21 Ceskoslovenska Akademie Ved Method and equipment for aerobic fermentation on liquid culture mediums
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US5182195A (en) * 1987-11-13 1993-01-26 Takeda Chemical Industries, Ltd. Method for increasing gene expression using protease deficient yeasts
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US5342763A (en) * 1992-11-23 1994-08-30 Genentech, Inc. Method for producing polypeptide via bacterial fermentation

Also Published As

Publication number Publication date
AU5946196A (en) 1996-09-05
WO1994012630A2 (en) 1994-06-09
AU679880B2 (en) 1997-07-10
PT668906E (pt) 2005-02-28
AU669414B2 (en) 1996-06-06
EP1524316A3 (de) 2005-04-27
JP3674705B2 (ja) 2005-07-20
EP1524316A2 (de) 2005-04-20
JP3513875B2 (ja) 2004-03-31
DE69334286D1 (de) 2009-07-09
ATE280223T1 (de) 2004-11-15
AU5615094A (en) 1994-06-22
DE69333671T2 (de) 2006-01-19
PT1524316E (pt) 2009-08-13
WO1994012630A3 (en) 1994-10-13
IL107712A (en) 2002-09-12
DK1524316T3 (da) 2009-09-07
EP0668906A1 (de) 1995-08-30
CA2147468C (en) 2006-05-16
ES2326802T3 (es) 2009-10-20
ES2231771T3 (es) 2005-05-16
EP0668906B1 (de) 2004-10-20
JPH08503611A (ja) 1996-04-23
US5342763A (en) 1994-08-30
EP1524316B1 (de) 2009-05-27
IL107712A0 (en) 1994-02-27
DK0668906T3 (da) 2005-02-07
ATE432344T1 (de) 2009-06-15
US5487980A (en) 1996-01-30
JP2004121256A (ja) 2004-04-22
CA2147468A1 (en) 1994-06-09
US5633165A (en) 1997-05-27

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