DE69432543T2 - Selektionmarker-genfreie rekombinante Stämme: Verfahren zur ihrer Herstellung und die Verwendung dieser Stämme - Google Patents

Selektionmarker-genfreie rekombinante Stämme: Verfahren zur ihrer Herstellung und die Verwendung dieser Stämme

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Publication number
DE69432543T2
DE69432543T2 DE69432543T DE69432543T DE69432543T2 DE 69432543 T2 DE69432543 T2 DE 69432543T2 DE 69432543 T DE69432543 T DE 69432543T DE 69432543 T DE69432543 T DE 69432543T DE 69432543 T2 DE69432543 T2 DE 69432543T2
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DE
Germany
Prior art keywords
strains
selection marker
preparation
marker gene
dna
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Expired - Lifetime
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DE69432543T
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English (en)
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DE69432543D1 (de
Inventor
Gerardus Cornelis Maria Selten
Gorcom Robertus Franciscus Van
Bart Willem Swinkels
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DSM IP Assets BV
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DSM NV
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Publication of DE69432543D1 publication Critical patent/DE69432543D1/de
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
DE69432543T 1993-07-23 1994-06-30 Selektionmarker-genfreie rekombinante Stämme: Verfahren zur ihrer Herstellung und die Verwendung dieser Stämme Expired - Lifetime DE69432543T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP93202195 1993-07-23

Publications (2)

Publication Number Publication Date
DE69432543D1 DE69432543D1 (de) 2003-05-28
DE69432543T2 true DE69432543T2 (de) 2003-12-24

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DE69432543T Expired - Lifetime DE69432543T2 (de) 1993-07-23 1994-06-30 Selektionmarker-genfreie rekombinante Stämme: Verfahren zur ihrer Herstellung und die Verwendung dieser Stämme

Country Status (13)

Country Link
US (3) US5876988A (de)
EP (2) EP1321523A3 (de)
JP (2) JP3833725B2 (de)
CN (1) CN100347301C (de)
AT (1) ATE238425T1 (de)
BR (1) BR9402912A (de)
CA (1) CA2128589C (de)
DE (1) DE69432543T2 (de)
DK (1) DK0635574T3 (de)
ES (1) ES2198410T3 (de)
FI (2) FI114481B (de)
IE (1) IE20030875A1 (de)
NZ (2) NZ299139A (de)

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NZ264065A (en) 1998-06-26
CN1098743A (zh) 1995-02-15
IE940591A1 (en) 1995-01-25
FI117388B (fi) 2006-09-29
ES2198410T3 (es) 2004-02-01
JP3833725B2 (ja) 2006-10-18
US5876988A (en) 1999-03-02
FI943485A0 (fi) 1994-07-22
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CA2128589A1 (en) 1995-01-24
CA2128589C (en) 2005-10-11
DE69432543D1 (de) 2003-05-28
ATE238425T1 (de) 2003-05-15
FI114481B (fi) 2004-10-29
AU690909B2 (en) 1998-05-07
DK0635574T3 (da) 2003-08-11
EP1321523A3 (de) 2004-03-03
FI20041124A (fi) 2004-08-27
CN100347301C (zh) 2007-11-07
BR9402912A (pt) 1995-04-11
JP2005296019A (ja) 2005-10-27
AU6862894A (en) 1995-02-02
US6063614A (en) 2000-05-16
EP0635574A1 (de) 1995-01-25
US6955909B1 (en) 2005-10-18
NZ299139A (en) 1998-06-26

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