DE69433425T2 - Zusammensetzungen und verfahren zur reinigung von nukleinsäuren - Google Patents

Zusammensetzungen und verfahren zur reinigung von nukleinsäuren Download PDF

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Publication number
DE69433425T2
DE69433425T2 DE69433425T DE69433425T DE69433425T2 DE 69433425 T2 DE69433425 T2 DE 69433425T2 DE 69433425 T DE69433425 T DE 69433425T DE 69433425 T DE69433425 T DE 69433425T DE 69433425 T2 DE69433425 T2 DE 69433425T2
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Prior art keywords
nucleic acid
silica materials
chaotropic salts
mixtures
nucleic acids
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Expired - Lifetime
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DE69433425T
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DE69433425D1 (de
Inventor
V Padhye
Chuck York
Adam Burkiewicz
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Promega Corp
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Promega Corp
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00Stock material or miscellaneous articles
    • Y10T428/29Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
    • Y10T428/2982Particulate matter [e.g., sphere, flake, etc.]
    • Y10T428/2991Coated
    • Y10T428/2993Silicic or refractory material containing [e.g., tungsten oxide, glass, cement, etc.]
    • Y10T428/2996Glass particles or spheres
DE69433425T 1993-08-30 1994-08-30 Zusammensetzungen und verfahren zur reinigung von nukleinsäuren Expired - Lifetime DE69433425T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11550493A 1993-08-30 1993-08-30
PCT/US1994/010014 WO1995006652A1 (en) 1993-08-30 1994-08-30 Nucleic acid purification compositions and methods

Publications (2)

Publication Number Publication Date
DE69433425D1 DE69433425D1 (de) 2004-01-29
DE69433425T2 true DE69433425T2 (de) 2004-10-07

Family

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Application Number Title Priority Date Filing Date
DE69433425T Expired - Lifetime DE69433425T2 (de) 1993-08-30 1994-08-30 Zusammensetzungen und verfahren zur reinigung von nukleinsäuren

Country Status (8)

Country Link
US (2) US5658548C1 (de)
EP (1) EP0723549B1 (de)
JP (2) JP3696238B2 (de)
AT (1) ATE256735T1 (de)
AU (1) AU689815B2 (de)
CA (1) CA2170604C (de)
DE (1) DE69433425T2 (de)
WO (1) WO1995006652A1 (de)

Families Citing this family (180)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19506887C2 (de) * 1995-02-17 1999-10-14 Invitek Gmbh Verfahren zur simultanen Isolierung von genomischer DNS und hochreiner Total RNS
EP1154443A1 (de) * 2000-05-12 2001-11-14 Boehringer Mannheim Gmbh Magnetische Glasteilchen, Herstellungsverfahen und Benützunge
KR100463475B1 (ko) * 1995-06-08 2005-06-22 로셰 디아그노스틱스 게엠베하 자기성피그먼트
DE19520398B4 (de) * 1995-06-08 2009-04-16 Roche Diagnostics Gmbh Magnetisches Pigment
DE69636553T2 (de) * 1995-07-07 2007-05-31 Toyo Boseki K.K. Nukleinsäuren bindender magnetischer Träger und Verfahren für die Nukleinsäureisolierung unter dessen Verwendung
JP2965131B2 (ja) * 1995-07-07 1999-10-18 東洋紡績株式会社 核酸結合用磁性担体およびそれを用いる核酸単離方法
US5783686A (en) * 1995-09-15 1998-07-21 Beckman Instruments, Inc. Method for purifying nucleic acids from heterogenous mixtures
US5620869A (en) * 1995-09-28 1997-04-15 Becton, Dickinson And Company Methods for reducing inhibition of nucleic acid amplification reactions
DE19600362C2 (de) * 1996-01-08 1998-11-12 Wolf Prof Dr Bertling Verfahren zur Erfassung und Quantifizierung von Nucleinsäure-Molekülen
EP0880537B1 (de) * 1996-02-14 2004-12-15 bioMerieux B.V. Isolierung von einzelsträngigen nukleinsäuren
NL1002781C1 (nl) * 1996-04-03 1997-10-06 Amsterdam Support Diagnostics Isolatie en/of amplificatie van hepatitis-C-virus-(HCV) -nucleïnezuren uit monsters waarvan vermoed wordt dat zij HCV bevatten.
US5981235A (en) * 1996-07-29 1999-11-09 Promega Corporation Methods for isolating nucleic acids using alkaline protease
WO1998006877A2 (en) * 1996-08-15 1998-02-19 Abbott Laboratories Method for removing amplification inhibitors from test samples
BR9806922A (pt) 1997-01-21 2000-04-18 Grace W R & Co Sìlica absorvente sobre substrato magnético
US20050287583A1 (en) * 1997-01-21 2005-12-29 Promega Corporation Methods and kits for isolating biological target materials using silica magnetic particles
US6027945A (en) 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
DE19702907A1 (de) 1997-01-28 1998-07-30 Boehringer Mannheim Gmbh Verfahren und Vorrichtung zur Reinigung von Nukleinsäuren
US6177559B1 (en) 1998-04-24 2001-01-23 Transgenomic, Inc. Process for separation of polynucleotide fragments
US6492144B1 (en) * 1997-05-30 2002-12-10 Diagen Corporation Methods for detection of nucleic acid sequences in urine
USRE39920E1 (en) 1997-05-30 2007-11-13 Xenomics, Inc. Methods for detection of nucleic acid sequences in urine
JP2002507121A (ja) * 1997-06-25 2002-03-05 プロメガ コーポレイション Rnaの単離方法
DE19743518A1 (de) * 1997-10-01 1999-04-15 Roche Diagnostics Gmbh Automatisierbare universell anwendbare Probenvorbereitungsmethode
DE19746874A1 (de) * 1997-10-23 1999-04-29 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen
JP4025399B2 (ja) * 1997-10-28 2007-12-19 株式会社日立製作所 核酸の回収方法及び装置
US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
AU2509799A (en) * 1997-12-18 1999-07-12 Invitek Gmbh Method for isolating short and long-chain nucleic acids
US6090359A (en) * 1998-02-17 2000-07-18 The Dow Chemical Company Process for hydrogenating aromatic polymers
US6265168B1 (en) * 1998-10-06 2001-07-24 Transgenomic, Inc. Apparatus and method for separating and purifying polynucleotides
JPH11266864A (ja) * 1998-03-19 1999-10-05 Hitachi Ltd 核酸の精製方法および精製用装置
WO1999051734A1 (en) * 1998-04-02 1999-10-14 Kyung Il Lee Glass microfiber column and method for the preparation and purification of plasmid dna using the same
US7078224B1 (en) 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
US6194562B1 (en) * 1998-04-22 2001-02-27 Promega Corporation Endotoxin reduction in nucleic acid purification
US6534262B1 (en) * 1998-05-14 2003-03-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
US6750059B1 (en) 1998-07-16 2004-06-15 Whatman, Inc. Archiving of vectors
JP2000166556A (ja) * 1998-12-10 2000-06-20 Hitachi Ltd 核酸の回収方法及び装置
ATE399215T1 (de) * 1999-03-11 2008-07-15 Whatman Inc Festmedium sowie verfahren zur speicherung und schnellen aufreinigung von nukleinsäuren
EP1621618B1 (de) 1999-05-14 2011-10-26 Promega Corporation Zell Konzentration und lysat Klärung mittels paramagnetischen Partikeln
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
US6270970B1 (en) * 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
DE19937607A1 (de) * 1999-08-09 2001-02-15 Bilatec Ges Zur Entwicklung Bi Reagenzienkit zur Isolierung von Nukleinsäuren
SK287932B6 (sk) * 1999-11-17 2012-05-03 Roche Diagnostics Gmbh Magnetic glass particles, method for their preparation and uses thereof
SE9904539D0 (sv) * 1999-12-10 1999-12-10 Alphahelix Ab Method and device for the handling of samples and reagents
US20020012990A1 (en) * 1999-12-22 2002-01-31 Lander Russel Jackson Process for the scaleable purification of plasmid DNA
DE50113146D1 (de) * 2000-02-04 2007-11-29 Qiagen Gmbh Nukleinsäure-isolierung aus stuhlproben und anderen biologischen materialien, die reich an inhibitoren sind
KR100356044B1 (ko) * 2000-07-07 2002-10-12 주식회사 지노타인 개질된 수정미세섬유막 및 그 이용
JP2004504330A (ja) * 2000-07-13 2004-02-12 インヴィトロジェン コーポレーション 溶解マトリクスを用いてタンパク質およびペプチドの迅速な抽出および単離のための方法および組成物
US6855817B2 (en) * 2000-07-21 2005-02-15 Mark B. Lyles Materials and methods for binding nucleic acids to surfaces
AU2001286499A1 (en) 2000-08-16 2002-02-25 Matthew Baker Transfusion medicine leukodepletion filter devices as a source of genetic material for genotyping studies
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7494817B2 (en) * 2000-09-06 2009-02-24 Transnet Yx, Inc. Methods for genotype screening using magnetic particles
US20050239125A1 (en) * 2000-09-06 2005-10-27 Hodge Timothy A Methods for genotype screening
US20050266494A1 (en) * 2000-09-06 2005-12-01 Hodge Timothy A System and method for computer network ordering of biological testing
US7011943B2 (en) 2000-09-06 2006-03-14 Transnetyx, Inc. Method for detecting a designated genetic sequence in murine genomic DNA
US20050272085A1 (en) * 2000-09-06 2005-12-08 Hodge Timothy A Methods for forensic and congenic screening
US20030027169A1 (en) * 2000-10-27 2003-02-06 Sheng Zhang One-well assay for high throughput detection of single nucleotide polymorphisms
EP1349951A4 (de) * 2001-01-09 2005-08-24 Whitehead Biomedical Inst Verfahren und reagenzien zur isolierung von nukleinsäuren
DE10125713A1 (de) * 2001-05-21 2002-11-28 Nimbus Biotechnologie Gmbh Immobilisierte asymmetrische Lipiddoppelschichten
GB0112868D0 (en) * 2001-05-25 2001-07-18 Secr Defence Detection system
US7556733B2 (en) 2001-06-15 2009-07-07 Mds Analytical Technologies (Us) Inc. Low volume filtration column devices and methods of filtering therewith
US7749388B2 (en) * 2001-06-15 2010-07-06 Life Technologies Corporation Low volume filtration column devices and methods of filtering therewith
US7229595B2 (en) * 2001-06-15 2007-06-12 Molecular Devices Corporation Filtration column devices and methods of filtering therewith
KR100454869B1 (ko) * 2001-07-13 2004-11-03 주식회사 인트론바이오테크놀로지 Dna 추출용 세포 용해 버퍼 및 이를 이용한 dna 추출방법
JP2002191351A (ja) * 2001-10-19 2002-07-09 Hitachi Ltd 核酸の精製用装置および核酸捕捉用チップ
ATE449186T1 (de) 2001-11-28 2009-12-15 Applied Biosystems Llc Zusammensetzungen und verfahren zur selektiven nukleinsäureisolierung
US20030203491A1 (en) * 2002-04-26 2003-10-30 Andrevski Zygmunt M. Gravitational flow purification system
DE10222133A1 (de) * 2002-05-17 2003-12-04 Gl Biotech Gmbh Verfahren zur Nukleinsäure-Extraktion und Nukleinsäure-Reinigung
GB0217963D0 (en) * 2002-08-02 2002-09-11 Cyclops Genome Sciences Ltd Purification of nucleic acids
ES2320875T5 (es) * 2002-10-04 2012-10-30 Ge Healthcare Bio-Sciences Corp. Procedimientos y materiales para utilizar compuestos químicos como herramienta para el almacenamiento de ácidos nucleicos sobre medios de sistemas de purificación de ácidos nucleicos
GB0223563D0 (en) * 2002-10-10 2002-11-20 Secr Defence Detection system
AU2003293015A1 (en) * 2002-11-26 2004-06-18 University Of Utah Research Foundation Microporous materials, methods, and articles for localizing and quantifying analytes
US7597936B2 (en) * 2002-11-26 2009-10-06 University Of Utah Research Foundation Method of producing a pigmented composite microporous material
US20040121336A1 (en) * 2002-12-20 2004-06-24 Greenfield I Lawrence Method for generating multiple samples containing a predetermined amount of nucleic acid
US20040197780A1 (en) * 2003-04-02 2004-10-07 Agencourt Bioscience Corporation Method for isolating nucleic acids
CN100395257C (zh) * 2003-05-08 2008-06-18 慈溪市中鼎生物技术有限公司 钾离子酸性水溶液和利用这种溶液的dna提取方法和试剂盒
US7238522B2 (en) * 2003-05-30 2007-07-03 Advisys, Inc. Devices and methods for biomaterial production
CN1878866B (zh) * 2003-07-25 2012-08-01 安比恩股份有限公司 用于从固定的样品中制备rna的方法和组合物
US20050059024A1 (en) * 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
US20140142290A1 (en) * 2003-08-01 2014-05-22 Life Technologies Corporation Compositions and methods for preparing short rna molecules and other nucleicacids
US20050054847A1 (en) * 2003-08-01 2005-03-10 Invitrogen Corporation Compositions and methods for preparing short RNA molecules and other nucleic acids
US7846333B2 (en) * 2003-11-24 2010-12-07 Effendorf AG Porous media
US20050181378A1 (en) * 2004-02-18 2005-08-18 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US20060160122A1 (en) * 2004-02-18 2006-07-20 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
RU2006139643A (ru) 2004-04-09 2008-05-20 Ресерч Тинк Тэнк, Инк. (Us) Способ сбора, хранения и транспортировки биологических образцов и устройство для его осуществления
DE102004021822B3 (de) 2004-04-30 2005-11-17 Siemens Ag Verfahren und Anordnung zur DNA-Amplifikation mittels PCR unter Einsatz von Trockenreagenzien
DE102004021780B4 (de) 2004-04-30 2008-10-02 Siemens Ag Verfahren und Anordnung zur DNA-Isolierung mit Trockenreagenzien
JP4476050B2 (ja) * 2004-06-30 2010-06-09 株式会社ニデック 視野計
JP4080462B2 (ja) * 2004-07-09 2008-04-23 株式会社日立製作所 核酸の回収方法
WO2006085987A2 (en) * 2004-07-09 2006-08-17 University Of Iowa Research Foundation Rna interference in respiratory epitheial cells
JP2008510456A (ja) * 2004-07-30 2008-04-10 アジェンコート バイオサイエンス コーポレーション 多官能基コート化固相担体を用いる核酸の単離方法
DE102005002343A1 (de) 2005-01-18 2006-07-27 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren zur spezifischen oder unspezifischen Separation von Zellen und/oder Viren aus flüssigen Medien und dessen Verwendung
US20060166223A1 (en) * 2005-01-26 2006-07-27 Reed Michael W DNA purification and analysis on nanoengineered surfaces
JP2006238822A (ja) * 2005-03-04 2006-09-14 Fuji Photo Film Co Ltd 核酸の分離精製方法
US7270794B2 (en) * 2005-03-30 2007-09-18 Shipley Larry W Process for recovering useful products and energy from siliceous plant matter
US20070015191A1 (en) * 2005-07-01 2007-01-18 Promega Corporation Network of buoyant particles for biomolecule purification and use of buoyant particles or network of buoyant particles for biomolecule purification
US7754873B2 (en) * 2005-07-16 2010-07-13 Zymo Research Corporation Isolation of nucleic acid using colored buffers
US20070129234A1 (en) * 2005-12-02 2007-06-07 Stockwell David M Lower temperature CO promoters for FCC with low NOx
WO2007070381A2 (en) 2005-12-09 2007-06-21 Promega Corporation Nucleic acid purification with a binding matrix
JP4825527B2 (ja) * 2006-02-03 2011-11-30 富士フイルム株式会社 核酸の分離精製方法
US7608399B2 (en) * 2006-06-26 2009-10-27 Blood Cell Storage, Inc. Device and method for extraction and analysis of nucleic acids from biological samples
US20080003585A1 (en) * 2006-06-29 2008-01-03 Bio-Rad Laboratories, Inc., A Corporation Of The State Of Delaware Purification and amplification of nucleic acids in a microfluidic device
US10131935B2 (en) * 2006-07-11 2018-11-20 Aj Innuscreen Gmbh Method for parallel isolation of viral nucleic acids
US7858363B2 (en) * 2006-07-22 2010-12-28 Zymo Research Corporation Plasmid DNA isolation
US7492312B2 (en) * 2006-11-14 2009-02-17 Fam Adly T Multiplicative mismatched filters for optimum range sidelobe suppression in barker code reception
KR100850430B1 (ko) * 2006-12-11 2008-08-05 (주)바이오니아 입자상 물질을 사용하는 핵산 분리 방법 및 핵산 분리용조성물
US8686129B2 (en) * 2007-03-20 2014-04-01 Agilent Technologies, Inc. Methods for the separation of biological molecules using sulfolane
CA2629589C (en) 2007-04-20 2016-03-29 F.Hoffmann-La Roche Ag Isolation and purification of nucleic acid molecules with a solid phase
WO2008150826A1 (en) * 2007-05-31 2008-12-11 Ge Healthcare Uk Limited Modified spin column for simple and rapid plasmid dna extraction
US20090048438A1 (en) * 2007-08-13 2009-02-19 Taigen Bioscience Corporation. Method for washing a column and method for extracting membrane-bound target molecules
ES2660785T3 (es) * 2007-08-22 2018-03-26 Trovagene, Inc. Métodos de uso de ARNmi para la detección de la muerte de células in vivo
JP4097687B2 (ja) * 2007-09-21 2008-06-11 株式会社日立製作所 核酸の回収方法
WO2009117167A1 (en) * 2008-01-02 2009-09-24 Blood Cell Storage, Inc. Devices and processes for nucleic acid extraction
FR2926560A1 (fr) * 2008-01-21 2009-07-24 Millipore Corp Procede d'extraction et de purification d'acides nucleiques sur membrane
US20110020924A1 (en) * 2008-04-30 2011-01-27 Gradalis, Inc. Highly pure plasmid dna preparations
US8546127B2 (en) * 2008-06-30 2013-10-01 General Electric Company Bacteria/RNA extraction device
US8158405B2 (en) * 2008-06-30 2012-04-17 General Electric Company Process for concentrating and processing fluid samples
CA2742598A1 (en) * 2008-11-04 2010-05-14 Blood Cell Storage, Inc. Nucleic acid extraction on curved glass surfaces
KR101569832B1 (ko) * 2008-11-19 2015-11-18 삼성전자주식회사 시료 중의 게놈 dna 및 플라스미드 dna를 서로 분리하는 방법 및 그를 위한 키트
JP2010158190A (ja) * 2009-01-07 2010-07-22 Hitachi High-Technologies Corp 核酸回収方法及び核酸回収装置
DE102009022512A1 (de) * 2009-05-25 2010-12-02 Qiagen Gmbh Verfahren zur Reaktivierung von Silikaoberflächen zur Isolierung von Nukleinsäuren
US8222397B2 (en) * 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US8697435B2 (en) * 2009-08-31 2014-04-15 Mbio Diagnostics, Inc. Integrated sample preparation and analyte detection
EP2345719A1 (de) * 2010-01-18 2011-07-20 Qiagen GmbH Verfahren zur Isolierung kleiner RNA
AT509622A1 (de) * 2010-04-08 2011-10-15 Greiner Bio One Gmbh Verfahren und kit zur trennung viraler und prokaryontischer von eukaryontischen nukleinsäuren
US20120004132A1 (en) * 2010-07-02 2012-01-05 Affymetrix, Inc. Detection of Nucleic Acids and Proteins
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US10167508B2 (en) 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US8756020B2 (en) 2011-01-25 2014-06-17 Ariosa Diagnostics, Inc. Enhanced risk probabilities using biomolecule estimations
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US8629264B2 (en) 2011-05-19 2014-01-14 Blood Cell Storage, Inc. Gravity flow fluidic device for nucleic acid extraction
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
US9803237B2 (en) 2012-04-24 2017-10-31 California Institute Of Technology Slip-induced compartmentalization
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
WO2014015269A1 (en) 2012-07-19 2014-01-23 Ariosa Diagnostics, Inc. Multiplexed sequential ligation-based detection of genetic variants
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9388465B2 (en) 2013-02-08 2016-07-12 10X Genomics, Inc. Polynucleotide barcode generation
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
RU2015114330A (ru) 2012-09-17 2016-11-10 У.Р. Грейс Энд Ко.-Конн. Хроматографические среды и устройства
CN102994494A (zh) * 2012-11-22 2013-03-27 成都福际生物技术有限公司 硅胶膜处理方法
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10640808B2 (en) 2013-03-13 2020-05-05 Abbott Molecular Inc. Systems and methods for isolating nucleic acids
CN103798227B (zh) * 2014-02-11 2016-03-16 中国农业科学院烟草研究所 一种锦紫苏类病毒离体常温保存方法
AU2015243445B2 (en) 2014-04-10 2020-05-28 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
WO2015157650A1 (en) * 2014-04-11 2015-10-15 Wako Pure Chemical Industries, Ltd Nucleic acid purification method
ES2929099T3 (es) 2014-05-02 2022-11-24 Grace W R & Co Material de soporte funcionalizado y métodos de fabricación y uso de material de soporte funcionalizado
EP4053292A1 (de) 2014-06-26 2022-09-07 10X Genomics, Inc. Verfahren zur analyse von nukleinsäuren aus einzelzellen oder zellpopulationen
CA2954576C (en) * 2014-07-09 2020-08-11 Daniel ENDERLE Methods for isolating microvesicles and extracting nucleic acids from biological samples
WO2016052386A1 (ja) * 2014-09-30 2016-04-07 東洋紡株式会社 核酸の分離精製方法および固相担体、デバイス、キット
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
WO2016109799A2 (en) * 2014-12-31 2016-07-07 The Rockefeller University Method of rna isolation from clinical samples
EP3262407B1 (de) 2015-02-24 2023-08-30 10X Genomics, Inc. Partitionsverarbeitungsverfahren und -systeme
US9422596B1 (en) 2015-04-27 2016-08-23 Norgen Biotek Corp. Methods and columns for nucleic acid purification
JP2018517559A (ja) 2015-06-05 2018-07-05 ダブリュー・アール・グレース・アンド・カンパニー−コーンW R Grace & Co−Conn 吸着性バイオプロセス清澄化剤並びにその製造及び使用方法
US11111488B2 (en) 2015-10-28 2021-09-07 Mirnagreen S.R.L. Methods for extracting bioactive small RNAs from plants and mushrooms
KR102336362B1 (ko) * 2016-03-03 2021-12-08 보이저 테라퓨틱스, 인크. 비-바이러스 유전자 전달을 위한 폐쇄형-말단 선형 듀플렉스 dna
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US11015186B2 (en) 2016-09-27 2021-05-25 Abbott Molecular Inc. Maximizing DNA yield of blood specimens collected in rapid clot tubes
WO2019035335A1 (ja) * 2017-08-18 2019-02-21 Agc株式会社 核酸の回収方法、核酸結合用担体および核酸回収キット
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
US11618684B2 (en) 2018-09-05 2023-04-04 Kilt, Llc Method for controlling the properties of biogenic silica
WO2020075186A1 (en) * 2018-10-08 2020-04-16 University Of Delhi South Campus Process for purifying emulsion pcr mixture, and implementations thereof
US11018156B2 (en) 2019-04-08 2021-05-25 Monolithic 3D Inc. 3D memory semiconductor devices and structures
US20220396826A1 (en) * 2019-11-20 2022-12-15 Toray Industries, Inc. Nucleic acid separation method, detection method, nucleic acid purification column and method of producing same
US11857981B2 (en) 2019-12-23 2024-01-02 10X Genomics, Inc. Magnetic separator for an automated single cell sequencing system
US11701668B1 (en) 2020-05-08 2023-07-18 10X Genomics, Inc. Methods and devices for magnetic separation
US11946038B1 (en) 2020-05-29 2024-04-02 10X Genomics, Inc. Methods and systems including flow and magnetic modules
JP7428326B2 (ja) 2021-11-02 2024-02-06 愛知県 検体中の核酸を回収する方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5075430A (en) * 1988-12-12 1991-12-24 Bio-Rad Laboratories, Inc. Process for the purification of DNA on diatomaceous earth
NL8900725A (nl) * 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
CA2067711C (en) * 1991-05-03 2000-08-08 Daniel Lee Woodard Solid phase extraction purification of dna
US5155018A (en) * 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
DE4139664A1 (de) * 1991-12-02 1993-06-03 Diagen Inst Molekularbio Vorrichtung und verfahren zur isolierung und reinigung von nukleinsaeuren

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US5808041A (en) 1998-09-15
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EP0723549B1 (de) 2003-12-17
US5658548A (en) 1997-08-19
WO1995006652A1 (en) 1995-03-09
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ATE256735T1 (de) 2004-01-15
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