DE69528375T2 - Chemilumineszente dialkylsubstituierte 1,2-dioxetanverbindungen, synthesemethode und anwendung - Google Patents

Chemilumineszente dialkylsubstituierte 1,2-dioxetanverbindungen, synthesemethode und anwendung

Info

Publication number
DE69528375T2
DE69528375T2 DE69528375T DE69528375T DE69528375T2 DE 69528375 T2 DE69528375 T2 DE 69528375T2 DE 69528375 T DE69528375 T DE 69528375T DE 69528375 T DE69528375 T DE 69528375T DE 69528375 T2 DE69528375 T2 DE 69528375T2
Authority
DE
Germany
Prior art keywords
chemiluminescent
substituted
dialkyl
dioxetane
application
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69528375T
Other languages
English (en)
Other versions
DE69528375D1 (de
Inventor
Paul Schaap
Hashem Akhavan-Tafti
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lumigen Inc
Original Assignee
Lumigen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lumigen Inc filed Critical Lumigen Inc
Application granted granted Critical
Publication of DE69528375D1 publication Critical patent/DE69528375D1/de
Publication of DE69528375T2 publication Critical patent/DE69528375T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C43/00—Ethers; Compounds having groups, groups or groups
    • C07C43/02—Ethers
    • C07C43/03—Ethers having all ether-oxygen atoms bound to acyclic carbon atoms
    • C07C43/14—Unsaturated ethers
    • C07C43/178—Unsaturated ethers containing hydroxy or O-metal groups
    • C07C43/1782—Unsaturated ethers containing hydroxy or O-metal groups containing six-membered aromatic rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C43/00—Ethers; Compounds having groups, groups or groups
    • C07C43/02—Ethers
    • C07C43/03—Ethers having all ether-oxygen atoms bound to acyclic carbon atoms
    • C07C43/14—Unsaturated ethers
    • C07C43/178—Unsaturated ethers containing hydroxy or O-metal groups
    • C07C43/1787—Unsaturated ethers containing hydroxy or O-metal groups containing six-membered aromatic rings and having unsaturation outside the aromatic rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C69/00—Esters of carboxylic acids; Esters of carbonic or haloformic acids
    • C07C69/017—Esters of hydroxy compounds having the esterified hydroxy group bound to a carbon atom of a six-membered aromatic ring
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D321/00—Heterocyclic compounds containing rings having two oxygen atoms as the only ring hetero atoms, not provided for by groups C07D317/00 - C07D319/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F7/00—Compounds containing elements of Groups 4 or 14 of the Periodic System
    • C07F7/02—Silicon compounds
    • C07F7/08—Compounds having one or more C—Si linkages
    • C07F7/18—Compounds having one or more C—Si linkages as well as one or more C—O—Si linkages
    • C07F7/1804—Compounds having Si-O-C linkages
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/08—Esters of oxyacids of phosphorus
    • C07F9/09—Esters of phosphoric acids
    • C07F9/12—Esters of phosphoric acids with hydroxyaryl compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/655—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms
    • C07F9/6551—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms the oxygen atom being part of a four-membered ring
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/655—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms
    • C07F9/6551—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms the oxygen atom being part of a four-membered ring
    • C07F9/65512—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms the oxygen atom being part of a four-membered ring condensed with carbocyclic rings or carbocyclic ring systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C2601/00—Systems containing only non-condensed rings
    • C07C2601/12—Systems containing only non-condensed rings with a six-membered ring
    • C07C2601/14—The ring being saturated
DE69528375T 1994-11-23 1995-11-02 Chemilumineszente dialkylsubstituierte 1,2-dioxetanverbindungen, synthesemethode und anwendung Expired - Lifetime DE69528375T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/344,124 US5578253A (en) 1994-11-23 1994-11-23 Chemiluminescent dialkyl-substituted 1,2-dioxetane compounds, methods of synthesis and use
PCT/US1995/014193 WO1996016137A1 (en) 1994-11-23 1995-11-02 Chemiluminescent dialkyl-substituted 1,2-dioxetane compounds, methods of synthesis and use

Publications (2)

Publication Number Publication Date
DE69528375D1 DE69528375D1 (de) 2002-10-31
DE69528375T2 true DE69528375T2 (de) 2003-04-30

Family

ID=23349156

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69528375T Expired - Lifetime DE69528375T2 (de) 1994-11-23 1995-11-02 Chemilumineszente dialkylsubstituierte 1,2-dioxetanverbindungen, synthesemethode und anwendung

Country Status (8)

Country Link
US (4) US5578253A (de)
EP (1) EP0794987B1 (de)
JP (1) JPH10509456A (de)
AT (1) ATE224938T1 (de)
AU (1) AU684409B2 (de)
CA (1) CA2203160A1 (de)
DE (1) DE69528375T2 (de)
WO (1) WO1996016137A1 (de)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5004565A (en) * 1986-07-17 1991-04-02 The Board Of Governors Of Wayne State University Method and compositions providing enhanced chemiluminescence from 1,2-dioxetanes
US5853974A (en) * 1995-06-07 1998-12-29 Chiron Corporation Enhancement of alkaline phosphatase with SDS in chemiluminescent substrates
US5777135A (en) * 1995-07-31 1998-07-07 Lumigen, Inc. Di-substituted 1,2-dioxetane compounds having increased water solubility and assay compositions
US6036892A (en) * 1995-07-31 2000-03-14 Lumigen, Inc. Water soluble tri-substituted 1,2-dioxetane compounds having increased storage stability, synthetic processes and intermediates
WO2000014092A1 (en) * 1998-09-08 2000-03-16 Giri Brij P Chemiluminescent 1,2-dioxetanes
US6783948B1 (en) * 1999-07-30 2004-08-31 Bayer Corporation Chemiluminescent acridinium compounds and analogues thereof as substrates of hydrolytic enzymes
EP1342724B1 (de) * 2002-03-08 2016-01-06 Tosoh Corporation 1,2-dioxetan derivate und diese gebrauchende reagenzien
US20040229294A1 (en) 2002-05-21 2004-11-18 Po-Ying Chan-Hui ErbB surface receptor complexes as biomarkers
US7402397B2 (en) 2002-05-21 2008-07-22 Monogram Biosciences, Inc. Detecting and profiling molecular complexes
US20040229380A1 (en) * 2002-05-21 2004-11-18 Po-Ying Chan-Hui ErbB heterodimers as biomarkers
US20040091850A1 (en) * 2002-11-08 2004-05-13 Travis Boone Single cell analysis of membrane molecules
US7390670B2 (en) 2003-02-20 2008-06-24 Lumigen, Inc. Signalling compounds and methods for detecting hydrogen peroxide
US20050019778A1 (en) * 2003-07-17 2005-01-27 Voyta John C. Sequential generation of multiple chemiluminescent signals on solid supports
US20050026151A1 (en) * 2003-07-17 2005-02-03 Voyta John C. Simultaneous generation of multiple chemiluminescent signals on solid supports
EP1681983A4 (de) * 2003-10-14 2008-12-10 Monogram Biosciences Inc Rezeptor-tyrosin-kinase-signalpfad-analyse für diagnose und therapie
US20050106577A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Cleavable solid phases for isolating nucleic acids
US20050106576A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Methods of using cleavable solid phases for isolating nucleic acids
US20060079699A1 (en) * 2004-08-27 2006-04-13 Brooks Edwards Intermediate compounds and methods for synthesizing chemiluminescent dioxetane substrates
WO2006116686A2 (en) * 2005-04-27 2006-11-02 The Trustees Of The University Of Pennsylvania Nanostructure enhanced luminescent devices
US7879575B2 (en) * 2005-04-27 2011-02-01 The Trustees Of The University Of Pennsylvania Nanostructures that provide a modified nanoenvironment for the enhancement of luminescence
WO2006116687A2 (en) * 2005-04-27 2006-11-02 The Trustees Of The University Of Pennsylvania Nanoassays
FR2886292B1 (fr) * 2005-05-27 2009-10-16 Quidd Soc Par Actions Simplifiee Biomarqueur a emission luminescente
KR20100042082A (ko) * 2008-10-15 2010-04-23 삼성전자주식회사 신호물질의 밀도가 증가되어 있는 고체 지지체, 그를 포함하는 키트 및 그를 이용한 표적물질 검출 방법
WO2010101839A2 (en) 2009-03-02 2010-09-10 Life Technologies Corporation Chemiluminescent compositions, methods, assays and kits for oxidative enzymes
CN103772433A (zh) * 2012-10-18 2014-05-07 深圳市美凯特科技有限公司 一种用于免疫分析的化学发光试剂amppd的合成方法
JP6204624B2 (ja) * 2017-04-10 2017-09-27 協立化学産業株式会社 過酸化物を用いた熱ラジカル重合開始剤及び熱硬化性樹脂組成物

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5068339A (en) * 1986-07-17 1991-11-26 Board Of Governors Of Wayne State University Enhanced chemiluminescence from 1,2-dioxetanes through energy transfer to tethered fluorescers
US4962192A (en) * 1986-07-17 1990-10-09 Board Of Governors Of Wayne State University Chemiluminescent 1,2-dioxetane compounds
US5004565A (en) * 1986-07-17 1991-04-02 The Board Of Governors Of Wayne State University Method and compositions providing enhanced chemiluminescence from 1,2-dioxetanes
US4959182A (en) * 1986-07-17 1990-09-25 Board Of Governors Of Wayne State University Method and compositions providing enhanced chemiluminescence from 1,2-dioxetanes
CA1340590C (en) * 1986-07-24 1999-06-08 John C. Voyta Chemiluminescence enhancement
US5220005A (en) * 1986-07-24 1993-06-15 Tropix, Inc. Substituted adamantyl dioxetanes
US5112960A (en) * 1989-07-17 1992-05-12 Bronstein Irena Y Chemiluminescent 3-(substituted adamant-2'-ylidene) 1,2-dioxetanes
DE3788203T2 (de) * 1986-07-24 1994-03-31 Tropix Inc Verfahren zum nachweis einer verbindung unter verwendung des enzymatisch induzierten zerfalls von dioxethanen.
US5625077A (en) * 1987-12-31 1997-04-29 Tropix, Inc. 1,2-dioxetanes
US4952707A (en) * 1988-06-30 1990-08-28 Tropix, Inc. Enzymatically-cleavable chemiluminescent fused polycyclic ring-containing 1,2-dioxetanes
US5132204A (en) * 1989-05-31 1992-07-21 Chiron Corporation Chemiluminescent double-triggered 1, 2-dioxetanes
US5326882A (en) * 1989-07-17 1994-07-05 Tropix, Inc. Chemiluminescent 3-(substituted Adamant-2'-Ylidene) 1,2-dioxetanes
US5547836A (en) * 1990-08-30 1996-08-20 Tropix, Inc. Enhancement of chemiluminescent assays
US5248618A (en) * 1991-06-05 1993-09-28 Life Technologies, Inc. Methods for generating light with chemiluminescent dioxetanes activated by anchimeric assisted cleavage
US5603868A (en) * 1992-10-30 1997-02-18 Abbott Laboratories Chemiluminescent electron-rich aryl-substituted 1,2-dioxetanes
KR0148439B1 (ko) * 1994-03-11 1998-11-02 마사까쓰 마쓰모또 1,2-디옥세탄 유도체
US5721370A (en) * 1995-07-31 1998-02-24 Lumigen Inc. Water soluble tri-substituted 1,2-dioxetane compounds and assay compositions having increased storage stability
US5777135A (en) * 1995-07-31 1998-07-07 Lumigen, Inc. Di-substituted 1,2-dioxetane compounds having increased water solubility and assay compositions

Also Published As

Publication number Publication date
EP0794987A4 (de) 1998-02-25
CA2203160A1 (en) 1996-05-30
ATE224938T1 (de) 2002-10-15
AU684409B2 (en) 1997-12-11
EP0794987B1 (de) 2002-09-25
EP0794987A1 (de) 1997-09-17
US5892064A (en) 1999-04-06
US6284899B1 (en) 2001-09-04
WO1996016137A1 (en) 1996-05-30
JPH10509456A (ja) 1998-09-14
DE69528375D1 (de) 2002-10-31
US5886238A (en) 1999-03-23
AU4141996A (en) 1996-06-17
US5578253A (en) 1996-11-26

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