DE69531666D1 - Verfahen zum Ausrichten von Makromolekülen durch Vorbeiführung eines Meniskus und Anwendung dafür - Google Patents

Verfahen zum Ausrichten von Makromolekülen durch Vorbeiführung eines Meniskus und Anwendung dafür

Info

Publication number
DE69531666D1
DE69531666D1 DE69531666T DE69531666T DE69531666D1 DE 69531666 D1 DE69531666 D1 DE 69531666D1 DE 69531666 T DE69531666 T DE 69531666T DE 69531666 T DE69531666 T DE 69531666T DE 69531666 D1 DE69531666 D1 DE 69531666D1
Authority
DE
Germany
Prior art keywords
dna
gene
macromolecule
reagents
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69531666T
Other languages
English (en)
Other versions
DE69531666T2 (de
Inventor
David Bensimon
Aaron Bensimon
Francois Heslot
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Institut Pasteur de Lille
Original Assignee
Centre National de la Recherche Scientifique CNRS
Institut Pasteur de Lille
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26230948&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69531666(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from FR9401574A external-priority patent/FR2716206B1/fr
Application filed by Centre National de la Recherche Scientifique CNRS, Institut Pasteur de Lille filed Critical Centre National de la Recherche Scientifique CNRS
Publication of DE69531666D1 publication Critical patent/DE69531666D1/de
Application granted granted Critical
Publication of DE69531666T2 publication Critical patent/DE69531666T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00387—Applications using probes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/0054—Means for coding or tagging the apparatus or the reagents
    • B01J2219/00572—Chemical means
    • B01J2219/00576—Chemical means fluorophore
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00608—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/0061—The surface being organic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/00626—Covalent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/00628—Ionic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/0063—Other, e.g. van der Waals forces, hydrogen bonding
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00632—Introduction of reactive groups to the surface
    • B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00677—Ex-situ synthesis followed by deposition on the substrate
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/969—Multiple layering of reactants
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/97—Test strip or test slide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
    • Y10S530/811—Peptides or proteins is immobilized on, or in, an inorganic carrier
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/145555—Hetero-N
DE69531666T 1994-02-11 1995-02-10 Verfahen zum Ausrichten von Makromolekülen durch Vorbeiführung eines Meniskus und Anwendung dafür Expired - Lifetime DE69531666T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
FR9401574 1994-02-11
FR9401574A FR2716206B1 (fr) 1994-02-11 1994-02-11 Surfaces hautement spécifiques pour réactions biologiques, procédé pour leur préparation et procédé de dosage d'une molécule utilisant ces surfaces.
FR9407444A FR2716263B1 (fr) 1994-02-11 1994-06-17 Procédé d'alignement de macromolécules par passage d'un ménisque et applications dans un procédé de mise en évidence, séparation et/ou dosage d'une macromolécule dans un échantillon.
FR9407444 1994-06-17
PCT/FR1995/000165 WO1995021939A1 (fr) 1994-02-11 1995-02-10 Procede d'alignement de macromolecules par passage d'un menisque et applications

Publications (2)

Publication Number Publication Date
DE69531666D1 true DE69531666D1 (de) 2003-10-09
DE69531666T2 DE69531666T2 (de) 2004-07-01

Family

ID=26230948

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69531666T Expired - Lifetime DE69531666T2 (de) 1994-02-11 1995-02-10 Verfahen zum Ausrichten von Makromolekülen durch Vorbeiführung eines Meniskus und Anwendung dafür
DE69531667T Expired - Lifetime DE69531667T2 (de) 1994-02-11 1995-02-10 Festphasen, spezifisch für biologische reaktionen, ihre herstellung und anwendung

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69531667T Expired - Lifetime DE69531667T2 (de) 1994-02-11 1995-02-10 Festphasen, spezifisch für biologische reaktionen, ihre herstellung und anwendung

Country Status (15)

Country Link
US (11) US6303296B1 (de)
EP (3) EP0743988B1 (de)
JP (2) JP3741719B2 (de)
KR (2) KR100424939B1 (de)
CN (2) CN1088110C (de)
AT (2) ATE249045T1 (de)
AU (2) AU699136B2 (de)
CA (2) CA2182905C (de)
DE (2) DE69531666T2 (de)
DK (2) DK0744028T3 (de)
ES (2) ES2206493T3 (de)
FR (1) FR2716263B1 (de)
NZ (2) NZ281255A (de)
PT (2) PT743988E (de)
WO (2) WO1995022056A1 (de)

Families Citing this family (121)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2716263B1 (fr) * 1994-02-11 1997-01-17 Pasteur Institut Procédé d'alignement de macromolécules par passage d'un ménisque et applications dans un procédé de mise en évidence, séparation et/ou dosage d'une macromolécule dans un échantillon.
US8142708B2 (en) * 1995-04-03 2012-03-27 Wisconsin Alumni Research Foundation Micro fluidic system for single molecule imaging
US7775368B2 (en) * 1995-04-03 2010-08-17 Wisconsin Alumni Research Foundation Micro-channel long molecule manipulation system
FR2737574B1 (fr) * 1995-08-03 1997-10-24 Pasteur Institut Appareillage d'alignement parallele de macromolecules et utilisation
WO1997006278A1 (en) * 1995-08-09 1997-02-20 Simon Adam J Molecular combing of macromolecules through the action of a meniscus on hihgly specific surfaces
AU7329096A (en) * 1995-11-13 1997-06-05 Institut Pasteur Ultrahigh resolution comparative nucleic acid hybridization to combed dna fibers
FR2755149B1 (fr) 1996-10-30 1999-01-15 Pasteur Institut Procede de diagnostic de maladies genetiques par peignage moleculaire et coffret de diagnostic
FR2755147B1 (fr) * 1996-10-30 1999-01-15 Pasteur Institut Procede de diagnostic de maladies genetiques par peignage moleculaire et coffret de diagnostic
US5830665A (en) * 1997-03-03 1998-11-03 Exact Laboratories, Inc. Contiguous genomic sequence scanning
WO1998041651A1 (en) * 1997-03-18 1998-09-24 Hsc Research & Development Limited Partnership Method for preparing chromatin
US6893877B2 (en) 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
DE69930726T2 (de) * 1998-01-12 2007-01-25 Massachusetts Institute Of Technology, Cambridge Verfahren und vorrichtung zur mikrotestdurchführung
US6114466A (en) * 1998-02-06 2000-09-05 Renal Tech International Llc Material for purification of physiological liquids of organism
WO2000006770A1 (en) * 1998-07-30 2000-02-10 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US20030022207A1 (en) * 1998-10-16 2003-01-30 Solexa, Ltd. Arrayed polynucleotides and their use in genome analysis
US20040106110A1 (en) * 1998-07-30 2004-06-03 Solexa, Ltd. Preparation of polynucleotide arrays
US20100130368A1 (en) * 1998-07-30 2010-05-27 Shankar Balasubramanian Method and system for sequencing polynucleotides
DE19853640C2 (de) * 1998-11-20 2002-01-31 Molecular Machines & Ind Gmbh Mehrgefäßanordnung mit verbesserter Empfindlichkeit für die optische Analytik, Verfahren zu ihrer Herstellung sowie ihre Verwendung in optischen Analyseverfahren
US6306578B1 (en) * 1999-03-19 2001-10-23 Genencor International, Inc. Multi-through hole testing plate for high throughput screening
US6573369B2 (en) * 1999-05-21 2003-06-03 Bioforce Nanosciences, Inc. Method and apparatus for solid state molecular analysis
US20030186311A1 (en) * 1999-05-21 2003-10-02 Bioforce Nanosciences, Inc. Parallel analysis of molecular interactions
US20020042081A1 (en) * 2000-10-10 2002-04-11 Eric Henderson Evaluating binding affinities by force stratification and force panning
US20030073250A1 (en) * 1999-05-21 2003-04-17 Eric Henderson Method and apparatus for solid state molecular analysis
US6248537B1 (en) 1999-05-28 2001-06-19 Institut Pasteur Use of the combing process for the identification of DNA origins of replication
AU5785400A (en) 1999-07-02 2001-01-22 Symyx Technologies, Inc. Polymer brushes for immobilizing molecules to a surface or substrate, where the polymers have water-soluble or water-dispersible segments and probes bonded thereto
US6762059B2 (en) * 1999-08-13 2004-07-13 U.S. Genomics, Inc. Methods and apparatuses for characterization of single polymers
US6927065B2 (en) 1999-08-13 2005-08-09 U.S. Genomics, Inc. Methods and apparatus for characterization of single polymers
US6696022B1 (en) 1999-08-13 2004-02-24 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
US6319674B1 (en) * 1999-09-16 2001-11-20 Agilent Technologies, Inc. Methods for attaching substances to surfaces
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
CA2400644C (en) 2000-02-18 2009-07-14 Board Of Trustees Of The Leland Stanford Junior University Apparatus and methods for parallel processing of micro-volume liquid reactions
US6897015B2 (en) * 2000-03-07 2005-05-24 Bioforce Nanosciences, Inc. Device and method of use for detection and characterization of pathogens and biological materials
US7008769B2 (en) * 2000-08-15 2006-03-07 Bioforce Nanosciences, Inc. Nanoscale molecular arrayer
AU2001296645A1 (en) 2000-10-06 2002-04-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding dna and rna
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US7205161B2 (en) * 2001-01-10 2007-04-17 Symyx Technologies, Inc. Polymer brushes for immobilizing molecules to a surface or substrate having improved stability
US6528117B2 (en) 2001-01-19 2003-03-04 Paul Lewis Method for coating a substance on one side of a substrate using a single miniscus
AU2002231934A1 (en) † 2001-01-30 2002-08-12 Solexa Ltd. Arrayed polynucleotides and their use in genome analysis
DK1370690T3 (da) * 2001-03-16 2012-07-09 Kalim Mir Arrays og fremgangsmåder til anvendelse heraf
US10272409B2 (en) * 2001-07-11 2019-04-30 Michael E. Hogan Methods and devices based upon a novel form of nucleic acid duplex on a surface
WO2003006676A2 (en) 2001-07-13 2003-01-23 Nanosphere, Inc. Method for immobilizing molecules onto surfaces
US20050287560A1 (en) * 2001-07-13 2005-12-29 Nanosphere, Inc. Method for preparing substrates having immobilized molecules and substrates
US7297553B2 (en) 2002-05-28 2007-11-20 Nanosphere, Inc. Method for attachment of silylated molecules to glass surfaces
WO2003008941A2 (en) * 2001-07-17 2003-01-30 Bioforce Nanosciences, Inc. Combined molecular blinding detection through force microscopy and mass spectrometry
US20030044798A1 (en) * 2001-08-31 2003-03-06 Lefkowitz Steven M. Methods for generating ligand arrays via deposition of ligands onto olefin displaying substrates, and arrays produced thereby
JP2005504698A (ja) * 2001-09-07 2005-02-17 ボーデン ケミカル インコーポレイテッド 接着促進剤を用いた被覆光ファイバと、その製造方法及び使用方法
US6916541B2 (en) 2001-09-07 2005-07-12 Penn State Research Foundation Modified substrates for the attachment of biomolecules
US7042488B2 (en) 2001-09-27 2006-05-09 Fujinon Corporation Electronic endoscope for highlighting blood vessel
US20050019488A1 (en) * 2001-11-30 2005-01-27 Cambridge Polymer Group, Inc., Boston, Ma Layered aligned polymer structures and methods of making same
US7048963B2 (en) * 2001-11-30 2006-05-23 Cambridge Polymers Group, Inc. Layered aligned polymer structures and methods of making same
US20030162181A1 (en) * 2002-02-28 2003-08-28 Eastman Kodak Company DNA sequencing and gene identification
US7094537B2 (en) * 2002-04-30 2006-08-22 Agilent Technologies, Inc. Micro arrays with structured and unstructured probes
WO2004048514A2 (en) * 2002-05-09 2004-06-10 U.S. Genomics, Inc. Methods for analyzing a nucleic acid
US20050239193A1 (en) * 2002-05-30 2005-10-27 Bioforce Nanosciences, Inc. Device and method of use for detection and characterization of microorganisms and microparticles
US20040031167A1 (en) * 2002-06-13 2004-02-19 Stein Nathan D. Single wafer method and apparatus for drying semiconductor substrates using an inert gas air-knife
US20030232916A1 (en) * 2002-06-14 2003-12-18 Lorah Dennis Paul Nonaqueous compositions
US6952651B2 (en) * 2002-06-17 2005-10-04 Intel Corporation Methods and apparatus for nucleic acid sequencing by signal stretching and data integration
WO2004007692A2 (en) * 2002-07-17 2004-01-22 U.S.Genomics, Inc. Methods and compositions for analyzing polymers using chimeric tags
US8277753B2 (en) 2002-08-23 2012-10-02 Life Technologies Corporation Microfluidic transfer pin
EP1407816B1 (de) * 2002-09-17 2008-05-21 Kalachev, Alexey Verfahren zum Anordnen einem Polymer-Molekül
CN1682237B (zh) * 2002-09-20 2010-05-26 英特尔公司 用于通过使用编码探针检测生物分子的方法和仪器
US7361821B2 (en) * 2002-09-20 2008-04-22 Intel Corporation Controlled alignment of nanobarcodes encoding specific information for scanning probe microscopy (SPM) reading
US7606403B2 (en) 2002-10-17 2009-10-20 Intel Corporation Model-based fusion of scanning probe microscopic images for detection and identification of molecular structures
CA2521999A1 (en) 2002-12-20 2004-09-02 Biotrove, Inc. Assay apparatus and method using microfluidic arrays
CN1745179A (zh) * 2003-01-02 2006-03-08 拜澳富斯毫微科学有限公司 在小样品体积中分子分析的方法和装置
US6861473B2 (en) 2003-02-28 2005-03-01 Baxter International Inc. Macromolecular ketoaldehydes
US20050123944A1 (en) * 2003-08-01 2005-06-09 U.S. Genomics, Inc. Methods and compositions related to the use of sequence-specific endonucleases for analyzing nucleic acids under non-cleaving conditions
EP1516665A1 (de) * 2003-09-18 2005-03-23 Sony International (Europe) GmbH Verfahren zu der Immobilisierung und dem Spannen einer Nukleinsäure auf ein Substrat
US20050064435A1 (en) * 2003-09-24 2005-03-24 Xing Su Programmable molecular barcodes
US20050136413A1 (en) * 2003-12-22 2005-06-23 Briggs Michael W. Reagent systems for biological assays
DE10361075A1 (de) * 2003-12-22 2005-07-28 Pac Tech - Packaging Technologies Gmbh Verfahren und Vorichtung zur Trocknung von Schaltungssubstraten
US20050147976A1 (en) * 2003-12-29 2005-07-07 Xing Su Methods for determining nucleotide sequence information
US20050151126A1 (en) * 2003-12-31 2005-07-14 Intel Corporation Methods of producing carbon nanotubes using peptide or nucleic acid micropatterning
CA2559171A1 (en) 2004-03-12 2005-09-29 Biotrove, Inc. Nanoliter array loading
EP1725587A2 (de) * 2004-03-19 2006-11-29 U.S. Genomics, Inc. Zusammensetzungen und verfahren zum nachweis von einzelnen molekülen
CN101010436B (zh) 2004-07-14 2012-12-05 Zs遗传学有限公司 分析核酸聚合物的系统和方法以及相关组件
US20060105453A1 (en) 2004-09-09 2006-05-18 Brenan Colin J Coating process for microfluidic sample arrays
US20060134679A1 (en) * 2004-12-17 2006-06-22 U.S. Genomics, Inc. Methods and compositions for acquiring information from unstretched polymer conformations
US7736818B2 (en) 2004-12-27 2010-06-15 Inphase Technologies, Inc. Holographic recording medium and method of making it
US20060223071A1 (en) * 2005-04-01 2006-10-05 Wisniewski Michele E Methods, compositions, and kits for detecting nucleic acids in a single vessel
US7319762B2 (en) * 2005-08-23 2008-01-15 Andrea Electronics Corporation Headset with flashing light emitting diodes
US8566038B2 (en) * 2005-10-21 2013-10-22 The Regents Of The University Of California Compositions and methods for analyzing immobilized nucleic acids
US20070178503A1 (en) * 2005-12-19 2007-08-02 Feng Jiang In-situ genomic DNA chip for detection of cancer
CA2640385C (en) 2005-12-23 2014-07-15 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
EP1963500B1 (de) * 2005-12-23 2013-02-13 Nanostring Technologies, Inc. Zusammensetzungen mit ausgerichteten und immobilisierten makromolekülen sowie verfahren zu ihrer herstellung
JP5081232B2 (ja) * 2006-05-22 2012-11-28 ナノストリング テクノロジーズ， インコーポレイテッド ナノレポーターを分析するためのシステムおよび方法
US7858305B2 (en) * 2006-06-30 2010-12-28 The Invention Science Fund I, Llc Method of combing a nucleic acid
JP5167449B2 (ja) * 2006-11-07 2013-03-21 独立行政法人科学技術振興機構 直鎖状核酸分子懸架支持体、直鎖状核酸分子伸長方法および直鎖状核酸分子標本
US7883869B2 (en) 2006-12-01 2011-02-08 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
CA2687292C (en) * 2007-04-10 2017-07-04 Nanostring Technologies, Inc. Methods and computer systems for identifying target-specific sequences for use in nanoreporters
WO2009052214A2 (en) * 2007-10-15 2009-04-23 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
EP2725107B1 (de) 2007-10-19 2018-08-29 The Trustees of Columbia University in the City of New York DNA-Sequenzierung mit nichtfluoreszierenden, reversiblen Nukleotidterminatoren und spaltbaren, fluoreszierenden ddNTPs und Nukleinsäuren die Inosine mit reversiblen Terminatorgruppe
EP2940029B1 (de) 2007-10-19 2023-11-29 The Trustees of Columbia University in the City of New York Design und synthese von spaltbaren fluoreszierenden nukleotiden als reversible terminatoren zur dna-sequenzierung durch synthese
JP5578596B2 (ja) * 2008-08-04 2014-08-27 独立行政法人物質・材料研究機構 高分子ナノワイヤの製造方法
EP2175037B1 (de) 2008-09-26 2017-10-11 Genomic Vision Verfahren zur Analyse von D4Z4-Tandemwiederholungsarrays von Nukleinsäure und Kit dafür
WO2010115122A2 (en) * 2009-04-03 2010-10-07 Illumina, Inc. Generation of uniform fragments of nucleic acids using patterned substrates
WO2010144128A2 (en) * 2009-06-08 2010-12-16 Zs Genetics, Inc. Molecular alignment and attachment of nucleic acid molecules
EP2561104B1 (de) 2010-04-23 2015-11-04 Genomic Vision Detektierung von hsv-dna-umlagerungen
ES2587434T3 (es) 2010-09-24 2016-10-24 Genomic Vision Método de detección, cuantificación y cartografía del daño y/o de la reparación de cadenas de ADN
FR2966844A1 (fr) 2010-11-03 2012-05-04 Vivatech Procede d'analyse genomique
WO2012125547A2 (en) * 2011-03-11 2012-09-20 Cornell University Systems and methods for high resolution biomolecular imaging and analysis
EP2714931A1 (de) 2011-06-03 2014-04-09 Genomic Vision Beurteilung des krebsrisikos auf der basis von rnu2-cnv und wechselwirkung zwischen rnu2 und brca1
WO2013087789A1 (en) 2011-12-13 2013-06-20 Glykos Finland Ltd. Antibody isoform arrays and methods thereof
WO2013101896A1 (en) * 2011-12-30 2013-07-04 Quest Diagnostics Investments Incorporated Nucleic acid analysis using emulsion pcr
EP2805281A4 (de) * 2012-01-18 2015-09-09 Singular Bio Inc Verfahren zur abbildung von strichcodierten molekülen zur erkennung von strukturvariationen und sequenzierungen
US9028776B2 (en) 2012-04-18 2015-05-12 Toxic Report Llc Device for stretching a polymer in a fluid sample
WO2014078652A1 (en) 2012-11-16 2014-05-22 Zs Genetics, Inc. Heavy atom labeled nucleosides, nucleotides, and nucleic acid polymers, and uses thereof
CA2900054A1 (en) * 2013-02-05 2014-08-14 Bionano Genomics, Inc. Methods for single-molecule analysis
US10648026B2 (en) 2013-03-15 2020-05-12 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
WO2014140788A1 (en) 2013-03-15 2014-09-18 Genomic Vision Methods for the detection of sequence amplification in the brca1 locus
CN105339506A (zh) 2013-03-15 2016-02-17 基因组影像公司 用于检测重排基因组序列中的断点的方法
EP3008209A2 (de) 2013-06-14 2016-04-20 Nanostring Technologies, Inc Multiplexierbares etikettbasiertes reportersystem
WO2017075179A1 (en) 2015-10-27 2017-05-04 Zs Genetics, Inc. Sequencing by deconvolution
US20180135080A1 (en) 2016-11-15 2018-05-17 Genomic Vision Sa Method for the monitoring of modified nucleases induced-gene editing events by molecular combing
WO2018100431A1 (en) 2016-11-29 2018-06-07 Genomic Vision Method for designing a set of polynucleotide sequences for analysis of specific events in a genetic region of interest
KR102077643B1 (ko) * 2018-02-09 2020-04-07 광운대학교 산학협력단 메니스커스 곡면을 갖는 시료 내 입자 분리 장치 및 방법
CN115997030A (zh) 2020-04-30 2023-04-21 德迈逊科技有限公司 用于大分子操作的装置和方法
EP4158064A1 (de) 2020-06-01 2023-04-05 Dimensiongen Vorrichtungen und verfahren zur genomanalyse
WO2022035729A1 (en) 2020-08-10 2022-02-17 Dimensiongen Devices and methods for multi-dimensional genome analysis
CN113514297A (zh) * 2021-06-21 2021-10-19 福建农林大学 一种用于单分子dna拉伸的磁控分子梳方法
WO2023055776A1 (en) 2021-09-29 2023-04-06 Michael David Austin Devices and methods for interrogating macromolecules

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4278651A (en) * 1978-09-27 1981-07-14 Becton Dickinson & Company Supported receptor and use thereof in an assay
NL8102178A (nl) * 1981-05-02 1982-12-01 Stichting Centraal Lab Werkwijze voor het aantonen van tegen bepaalde antigenen gerichte antistoffen, alsmede inrichting en reagentia voor het uitvoeren van deze werkwijze.
US4698302A (en) * 1983-05-12 1987-10-06 Advanced Magnetics, Inc. Enzymatic reactions using magnetic particles
US4554088A (en) * 1983-05-12 1985-11-19 Advanced Magnetics Inc. Magnetic particles for use in separations
GB8314523D0 (en) * 1983-05-25 1983-06-29 Lowe C R Diagnostic device
US4539061A (en) * 1983-09-07 1985-09-03 Yeda Research And Development Co., Ltd. Process for the production of built-up films by the stepwise adsorption of individual monolayers
US4724207A (en) * 1984-02-02 1988-02-09 Cuno Incorporated Modified siliceous chromatographic supports
US4794168A (en) * 1984-04-24 1988-12-27 Scripps Clinic And Research Foundation Leukemia-associated virus immunogen, vaccine and assay
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4803170A (en) * 1985-05-09 1989-02-07 Ultra Diagnostics Corporation Competitive immunoassay method, device and test kit
US4722890A (en) * 1985-08-27 1988-02-02 The United States Of America As Represented By The Department Of Health And Human Services Quantitative assay for human terminal complement cascade activation
US5447841A (en) * 1986-01-16 1995-09-05 The Regents Of The Univ. Of California Methods for chromosome-specific staining
US4709037A (en) * 1987-02-17 1987-11-24 Hoechst Celanese Corporation Biotinylating agents
DE3717210C2 (de) * 1987-05-22 1993-11-25 Diagen Inst Molekularbio Verfahren zur Modifizierung von Nukleinsäuren
US5098977A (en) * 1987-09-23 1992-03-24 Board Of Regents, The University Of Texas System Methods and compositions for providing articles having improved biocompatability characteristics
US4921809A (en) * 1987-09-29 1990-05-01 Findley Adhesives, Inc. Polymer coated solid matrices and use in immunoassays
FR2634023B1 (fr) * 1988-07-08 1994-03-25 Bio Merieux Reactif sous forme de support solide permettant de fixer par covalence un ligand biologique amine, sa preparation et son utilisation
US5102798A (en) * 1988-09-08 1992-04-07 Allage Associates Surface functionalized Langmuir-Blodgett films for immobilization of active moieties
US5237016A (en) * 1989-01-05 1993-08-17 Siska Diagnostics, Inc. End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
JP2910929B2 (ja) * 1989-03-23 1999-06-23 浜松ホトニクス株式会社 磁気粒子よりなる粒子の表面改質方法
EP0391674B1 (de) * 1989-04-05 1996-03-20 New York University Verfahren zur Charakterisierung von Teilchen
US5240846A (en) * 1989-08-22 1993-08-31 The Regents Of The University Of Michigan Gene therapy vector for cystic fibrosis
US5232829A (en) * 1989-09-29 1993-08-03 Hoffmann-La Roche Inc. Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes
US5215882A (en) * 1989-11-30 1993-06-01 Ortho Diagnostic Systems, Inc. Method of immobilizing nucleic acid on a solid surface for use in nucleic acid hybridization assays
FR2656319B1 (fr) * 1989-12-27 1992-03-20 Rhone Poulenc Chimie Microspheres composites magnetisables a base d'un polymere organosilicie reticule, leur procede de preparation et leur application en biologie.
US5079169A (en) * 1990-05-22 1992-01-07 The Regents Of The Stanford Leland Junior University Method for optically manipulating polymer filaments
US5200315A (en) * 1990-07-25 1993-04-06 Eastman Kodak Company Particulate biologically active reagent containing polyoxyalkylene side chains, analytical element and methods for use of the reagent
US5244796A (en) * 1990-10-12 1993-09-14 Syracuse University Cloned leuconostoc mesenteroides glucose-6-phosphate dehydrogenase genes and method of making glucose-6-phospate dehydrogenase
US5474796A (en) * 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
EP0640147A1 (de) * 1992-05-07 1995-03-01 Ig Laboratories Inc. Verfahren zur Hochauflösenden Genkartierung
CA2098960C (en) * 1992-07-10 2004-11-02 Richard Barner Bio specifically recognizing surfaces on solid supports and method for their preparation
US5372930A (en) * 1992-09-16 1994-12-13 The United States Of America As Represented By The Secretary Of The Navy Sensor for ultra-low concentration molecular recognition
US5610287A (en) * 1993-12-06 1997-03-11 Molecular Tool, Inc. Method for immobilizing nucleic acid molecules
FR2716263B1 (fr) * 1994-02-11 1997-01-17 Pasteur Institut Procédé d'alignement de macromolécules par passage d'un ménisque et applications dans un procédé de mise en évidence, séparation et/ou dosage d'une macromolécule dans un échantillon.
US5624711A (en) 1995-04-27 1997-04-29 Affymax Technologies, N.V. Derivatization of solid supports and methods for oligomer synthesis
WO1997006278A1 (en) 1995-08-09 1997-02-20 Simon Adam J Molecular combing of macromolecules through the action of a meniscus on hihgly specific surfaces
US5554088A (en) * 1995-09-12 1996-09-10 Zlojutro; Milan Sport training bag and support
US5851769A (en) 1995-09-27 1998-12-22 The Regents Of The University Of California Quantitative DNA fiber mapping

Also Published As

Publication number Publication date
CA2182905C (fr) 2010-08-17
KR100424939B1 (ko) 2004-07-27
US20020031774A1 (en) 2002-03-14
FR2716263B1 (fr) 1997-01-17
EP0743988A1 (de) 1996-11-27
US6265153B1 (en) 2001-07-24
US6548255B2 (en) 2003-04-15
EP1369494A3 (de) 2003-12-17
AU1712095A (en) 1995-08-29
US6130044A (en) 2000-10-10
DE69531667D1 (de) 2003-10-09
US5846724A (en) 1998-12-08
CN1144561A (zh) 1997-03-05
KR100395018B1 (ko) 2003-08-19
AU1814595A (en) 1995-08-29
PT743988E (pt) 2004-01-30
ES2206494T3 (es) 2004-05-16
JP3741718B2 (ja) 2006-02-01
ATE249045T1 (de) 2003-09-15
NZ281255A (en) 1998-05-27
CA2182906C (fr) 2006-08-15
EP1369494A2 (de) 2003-12-10
US6294324B1 (en) 2001-09-25
DK0743988T3 (da) 2004-01-05
CN1144540A (zh) 1997-03-05
US20060257910A1 (en) 2006-11-16
US5677126A (en) 1997-10-14
DE69531666T2 (de) 2004-07-01
WO1995021939A1 (fr) 1995-08-17
US20030175779A1 (en) 2003-09-18
AU699136B2 (en) 1998-11-26
CN1125342C (zh) 2003-10-22
ES2206493T3 (es) 2004-05-16
US7122647B2 (en) 2006-10-17
JPH09509056A (ja) 1997-09-16
PT744028E (pt) 2004-01-30
FR2716263A1 (fr) 1995-08-18
US6303296B1 (en) 2001-10-16
EP0744028B1 (de) 2003-09-03
CA2182905A1 (fr) 1995-08-17
US6054327A (en) 2000-04-25
DK0744028T3 (da) 2003-12-22
EP0743988B1 (de) 2003-09-03
CA2182906A1 (fr) 1995-08-17
ATE248927T1 (de) 2003-09-15
US5840862A (en) 1998-11-24
US7754425B2 (en) 2010-07-13
JPH09509057A (ja) 1997-09-16
NZ279861A (en) 1998-07-28
JP3741719B2 (ja) 2006-02-01
DE69531667T2 (de) 2004-06-24
CN1088110C (zh) 2002-07-24
WO1995022056A1 (fr) 1995-08-17
EP0744028A1 (de) 1996-11-27

Similar Documents

Publication Publication Date Title
DE69531666D1 (de) Verfahen zum Ausrichten von Makromolekülen durch Vorbeiführung eines Meniskus und Anwendung dafür
Angelis et al. Single cell gel electrophoresis: detection of DNA damage at different levels of sensitivity
ES2534139T3 (es) Métodos y ácidos nucleicos para el análisis de la expresión génica asociada con el pronóstico de trastornos proliferativos de células de próstata
ES8507177A1 (es) Un metodo para detectar bacterias en una muestra a ensayar
ES2180207T3 (es) Deteccion e identificacion del papilomavirus humano por medio de pcr y de hibridacion inversa de tipo especifico.
DE60128908D1 (de) Verfahren zum Nachweis und zur Lokalisierung von Genen in situ durch Hybridisierung einer verzweigten DNA
US10392646B2 (en) Device and methods of using device for detection of aminoacidopathies
ES8605862A1 (es) Perfeccionamientos en las disposiciones para la deteccion desecuencias determinadas de nucleotidos, procedimiento de deteccion y de caracterizacion de una secuencia o de un fragmento de acido nucleico determinado y sonda clonad
US20170198329A1 (en) Device and methods of using device for detection of aminoacidopathies
RU2270254C2 (ru) Способ идентификации трансгенных последовательностей днк в растительном материале и продуктах на его основе, набор олигонуклеотидов и биочип для осуществления этого способа
CN104673934A (zh) 锦鲤疱疹病毒的rt-lamp检测引物组、试剂盒及其检测方法
DE60044294D1 (de) Diagnostische tests auf prokaryotische und eukaryotische organismen, basierend auf nukleinsäuresonden
CN101195842B (zh) 一种检测牛x、y精子分离纯度的引物
CN104152448A (zh) 黑麦染色体寡核苷酸探针及其制备方法
WO1998016632B1 (en) MATERIALS AND METHODS FOR DETECTION OF $i(OXALOBACTER)
CN101240338A (zh) 检测基因突变的方法
CN108411030A (zh) 引物对及包含其的试剂盒、用途和检测蒺藜苜蓿生态型a17和r108的方法
CN109234432A (zh) 一种基于重组酶聚合酶扩增方法检测大豆猝倒病的引物、探针和试剂盒
CN103898221A (zh) 恶臭假单胞菌的pcr检测用引物及检测方法
US7588921B1 (en) Messenger RNA profiling: body fluid identification using multiplex real time-polymerase chain reaction (q-PCR)
WO2006121277A1 (en) Fret probes for fluorescent detection of the epsps gene
CN105177123A (zh) 锌铜超氧化物歧化酶sod1表达量的分子检测方法及引物
Vrba et al. Interspecies variation of tetrazolium oxidase electrophoretic patterns in mycobacteria
KR100470960B1 (ko) 유전자 손상 및 산화적 손상을 동시에 탐지할 수 있는재조합 박테리아
CN117286288A (zh) 一种h5n1亚型禽流感病毒rpa的检测引物组、试剂盒及应用

Legal Events

Date Code Title Description
8364 No opposition during term of opposition