DE69613856D1 - Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität - Google Patents

Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität

Info

Publication number
DE69613856D1
DE69613856D1 DE69613856T DE69613856T DE69613856D1 DE 69613856 D1 DE69613856 D1 DE 69613856D1 DE 69613856 T DE69613856 T DE 69613856T DE 69613856 T DE69613856 T DE 69613856T DE 69613856 D1 DE69613856 D1 DE 69613856D1
Authority
DE
Germany
Prior art keywords
dna polymerase
enzyms
muted
derived
exonuclease activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69613856T
Other languages
English (en)
Other versions
DE69613856T2 (de
Inventor
A Mamone
Maria Davis
Dan Sha
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Global Life Sciences Solutions USA LLC
Original Assignee
Amersham Life Science Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Life Science Inc filed Critical Amersham Life Science Inc
Application granted granted Critical
Publication of DE69613856D1 publication Critical patent/DE69613856D1/de
Publication of DE69613856T2 publication Critical patent/DE69613856T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE69613856T 1995-12-15 1996-12-13 Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität Expired - Lifetime DE69613856T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US868895P 1995-12-15 1995-12-15
PCT/US1996/020225 WO1997021821A1 (en) 1995-12-15 1996-12-13 Thermostable dna polymerase from thermoanaerobacter thermohydrosulfuricus and mutant enzymes thereof with exonuclease activity removed

Publications (2)

Publication Number Publication Date
DE69613856D1 true DE69613856D1 (de) 2001-08-16
DE69613856T2 DE69613856T2 (de) 2002-04-04

Family

ID=21733102

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69613856T Expired - Lifetime DE69613856T2 (de) 1995-12-15 1996-12-13 Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität

Country Status (8)

Country Link
US (1) US5744312A (de)
EP (1) EP0866868B1 (de)
JP (1) JP4073038B2 (de)
AT (1) ATE203056T1 (de)
CA (1) CA2240334C (de)
DE (1) DE69613856T2 (de)
ES (1) ES2162132T3 (de)
WO (1) WO1997021821A1 (de)

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EP0921196A1 (de) 1997-12-02 1999-06-09 Roche Diagnostics GmbH Modifizierte DNA Polymerase aus Carboxydothermus hydrogenoformans und deren Verwendung für gekoppelte reverse Transkription und Polymerase-kettenreaktion
JP2002506626A (ja) * 1998-03-18 2002-03-05 アマーシャム・ファルマシア・バイオテック・インコーポレーテッド Ｔｈｅｍｏａｎａｅｒｏｂａｃｔｅｒｔｈｅｒｍｏｈｙｄｒｏｓｕｌｆｒｉｃｕｓ由来の熱安定性ＤＮＡポリメラーゼ
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
EP1086209B1 (de) 1998-06-17 2007-08-22 Amersham Biosciences Corp. Fy7 polymerase
US6406891B1 (en) 1998-09-28 2002-06-18 Board Of Regents, The University Of Texas System Dual RT procedure for cDNA synthesis
US6238868B1 (en) * 1999-04-12 2001-05-29 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
CN102586228A (zh) 1999-09-13 2012-07-18 纽亘技术公司 用于多核苷酸序列线性等温扩增的方法及组合物
US6692918B2 (en) * 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
DE60141087D1 (de) 2000-06-26 2010-03-04 Nugen Technologies Inc Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren
US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
KR20000072141A (ko) * 2000-08-04 2000-12-05 김유삼 신규한 호열성 단백질 분해효소의 ｄｎａ 서열 및그로부터 유래된 단백질
US6858413B2 (en) * 2000-12-13 2005-02-22 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US6794141B2 (en) 2000-12-22 2004-09-21 Arcturus Bioscience, Inc. Nucleic acid amplification
BR0205268A (pt) 2001-03-09 2004-11-30 Nugen Technologies Inc Processos e composições para a mplificação de sequências de rna
EP1390537B1 (de) * 2001-03-09 2013-11-13 Nugen Technologies, Inc. Verfahren und zusammensetzungen zur amplifikation von rna-sequenzen
CA2440754A1 (en) 2001-03-12 2002-09-19 Stephen Quake Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
KR20010079080A (ko) * 2001-06-12 2001-08-22 김유삼 신규한 dna 중합효소의 dna 서열 및 그로부터 유래된 단백질
CA2460546A1 (en) * 2001-09-14 2003-03-27 Invitrogen Corporation Dna polymerases and mutants thereof
CN1604907A (zh) * 2001-12-20 2005-04-06 阿默森生物科学有限公司 通过热硫化氢热厌氧杆菌dna聚合酶i变体进行的核酸标记
US20030180741A1 (en) 2001-12-21 2003-09-25 Holly Hogrefe High fidelity DNA polymerase compositions and uses therefor
AU2003220249A1 (en) * 2002-03-15 2003-09-29 Arcturus Bioscience, Inc. Improved nucleic acid amplification
DE10315640A1 (de) * 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
CA2521084A1 (en) 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
US7169560B2 (en) * 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US7981604B2 (en) 2004-02-19 2011-07-19 California Institute Of Technology Methods and kits for analyzing polynucleotide sequences
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US20050260609A1 (en) * 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
JP2008512084A (ja) 2004-05-25 2008-04-24 ヘリコス バイオサイエンシーズ コーポレイション 核酸の配列決定のための方法およびデバイス
GB2416352B (en) * 2004-07-21 2009-01-28 Bioline Ltd A method for performing the hot start of enzymatic reactions
BRPI0515777A (pt) * 2004-12-11 2008-08-05 Cytogenix Inc biossìntese isenta de células de ácido nucléico de alta qualidade e usos dos mesmos
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
WO2007030759A2 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
US8093030B2 (en) * 2005-10-06 2012-01-10 Lucigen Corporation Thermostable viral polymerases and methods of use
WO2007070642A2 (en) * 2005-12-15 2007-06-21 Helicos Biosciences Corporation Methods for increasing accuracy of nucleic acid sequencing
WO2007117331A2 (en) * 2005-12-22 2007-10-18 Ge Healthcare Bio-Sciences Corp. Novel dna polymerase from thermoanaerobacter tengcongenesis
US7397546B2 (en) * 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US20080309926A1 (en) * 2006-03-08 2008-12-18 Aaron Weber Systems and methods for reducing detected intensity non uniformity in a laser beam
US20100003189A1 (en) 2006-07-14 2010-01-07 The Regents Of The University Of California Cancer biomarkers and methods of use thereof
US20090203531A1 (en) * 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
WO2009117698A2 (en) 2008-03-21 2009-09-24 Nugen Technologies, Inc. Methods of rna amplification in the presence of dna
EP3508854A1 (de) 2010-04-27 2019-07-10 The Regents of The University of California Krebsbiomarker und verfahren zur verwendung davon
US9777319B2 (en) 2012-06-29 2017-10-03 General Electric Company Method for isothermal DNA amplification starting from an RNA template
US11543411B2 (en) 2014-12-05 2023-01-03 Prelude Corporation DCIS recurrence and invasive breast cancer
WO2020056338A1 (en) 2018-09-14 2020-03-19 Prelude Corporation Method of selection for treatment of subjects at risk of invasive breast cancer
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EP4083204A1 (de) 2021-04-30 2022-11-02 Wageningen Universiteit Genweite randomisierung
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US5405774A (en) * 1986-08-22 1995-04-11 Hoffmann-La Roche Inc. DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17
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EP0894860B1 (de) * 1990-09-28 2012-12-12 F. Hoffmann-La Roche AG Thermostabile DNA Polymerasen und deren Deletionsmutanten
ATE143057T1 (de) * 1994-10-17 1996-10-15 Harvard College Dns polymerase mit veränderter nukleotid- bindungstelle

Also Published As

Publication number Publication date
EP0866868A1 (de) 1998-09-30
EP0866868B1 (de) 2001-07-11
ATE203056T1 (de) 2001-07-15
DE69613856T2 (de) 2002-04-04
JP4073038B2 (ja) 2008-04-09
WO1997021821A1 (en) 1997-06-19
CA2240334A1 (en) 1997-06-19
ES2162132T3 (es) 2001-12-16
CA2240334C (en) 2008-07-22
US5744312A (en) 1998-04-28
JP2000501616A (ja) 2000-02-15

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Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: AMERSHAM PHARMACIA BIOTECH INC., PISCATAWAY, N.J.,

8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: GE HEALTHCARE BIO-SCIENCES CORP. (N.D.GES.D.STAATE