DE69621507D1 - Verfahren zur molekularen Indexierung von Genen unter Verwendung von Restriktionsenzymen - Google Patents

Verfahren zur molekularen Indexierung von Genen unter Verwendung von Restriktionsenzymen

Info

Publication number
DE69621507D1
DE69621507D1 DE69621507T DE69621507T DE69621507D1 DE 69621507 D1 DE69621507 D1 DE 69621507D1 DE 69621507 T DE69621507 T DE 69621507T DE 69621507 T DE69621507 T DE 69621507T DE 69621507 D1 DE69621507 D1 DE 69621507D1
Authority
DE
Germany
Prior art keywords
genes
restriction enzymes
molecular indexing
indexing
molecular
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69621507T
Other languages
English (en)
Other versions
DE69621507T2 (de
Inventor
Kikuya Kato
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japan Science and Technology Agency
Original Assignee
Japan Science and Technology Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP7184006A external-priority patent/JP2763277B2/ja
Priority claimed from JP7234122A external-priority patent/JP2763278B2/ja
Application filed by Japan Science and Technology Corp filed Critical Japan Science and Technology Corp
Publication of DE69621507D1 publication Critical patent/DE69621507D1/de
Application granted granted Critical
Publication of DE69621507T2 publication Critical patent/DE69621507T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855Ligating adaptors
DE69621507T 1995-03-28 1996-03-26 Verfahren zur molekularen Indexierung von Genen unter Verwendung von Restriktionsenzymen Expired - Fee Related DE69621507T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP6969595 1995-03-28
JP7184006A JP2763277B2 (ja) 1995-07-20 1995-07-20 Dna分子索引法
JP7234122A JP2763278B2 (ja) 1995-03-28 1995-09-12 Dna分子索引法

Publications (2)

Publication Number Publication Date
DE69621507D1 true DE69621507D1 (de) 2002-07-11
DE69621507T2 DE69621507T2 (de) 2003-01-09

Family

ID=27300117

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69621507T Expired - Fee Related DE69621507T2 (de) 1995-03-28 1996-03-26 Verfahren zur molekularen Indexierung von Genen unter Verwendung von Restriktionsenzymen

Country Status (4)

Country Link
US (1) US5707807A (de)
EP (1) EP0735144B1 (de)
AU (1) AU692685B2 (de)
DE (1) DE69621507T2 (de)

Families Citing this family (98)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69333650T2 (de) * 1992-02-19 2006-01-12 The Public Health Research Institute Of The City Of New York, Inc. Neue anordnungn von oligonukleotiden und ihr nutzen zum sortieren, isolieren, sequenzieren und manipulieren von nukleinsäuren
US6379897B1 (en) 2000-11-09 2002-04-30 Nanogen, Inc. Methods for gene expression monitoring on electronic microarrays
US5710000A (en) * 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
USRE43097E1 (en) 1994-10-13 2012-01-10 Illumina, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US6013445A (en) * 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
DE19518505A1 (de) * 1995-05-19 1996-11-21 Max Planck Gesellschaft Verfahren zur Genexpressionsanalyse
US5972693A (en) * 1995-10-24 1999-10-26 Curagen Corporation Apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing
GB9618544D0 (en) * 1996-09-05 1996-10-16 Brax Genomics Ltd Characterising DNA
IL130885A (en) * 1997-01-15 2005-05-17 Xzillion Gmbh & Co Kg Hybridisation probes comprising a base sequence cleavably linked to a mass label and uses thereof
US6699668B1 (en) 1997-01-15 2004-03-02 Xzillion Gmbh & Co. Mass label linked hybridisation probes
US5994068A (en) * 1997-03-11 1999-11-30 Wisconsin Alumni Research Foundation Nucleic acid indexing
ES2563643T3 (es) 1997-04-01 2016-03-15 Illumina Cambridge Limited Método de secuenciación de ácido nucleico
US6090556A (en) * 1997-04-07 2000-07-18 Japan Science & Technology Corporation Method for quantitatively determining the expression of a gene
GB9707980D0 (en) * 1997-04-21 1997-06-11 Brax Genomics Ltd Characterising DNA
JP2002502237A (ja) * 1997-05-12 2002-01-22 ライフ テクノロジーズ,インコーポレイテッド 核酸分子の生成および精製のための方法
US6670120B1 (en) 1997-07-11 2003-12-30 Xzillion Gmbh & Co. Categorising nucleic acid
GB9714716D0 (en) * 1997-07-11 1997-09-17 Brax Genomics Ltd Characterising nucleic acids
US6399334B1 (en) 1997-09-24 2002-06-04 Invitrogen Corporation Normalized nucleic acid libraries and methods of production thereof
DE19806431C1 (de) * 1998-02-17 1999-10-14 Novartis Ag Neues Verfahren zur Identifikation und Charakterisierung von mRNA-Molekülen
US6218122B1 (en) * 1998-06-19 2001-04-17 Rosetta Inpharmatics, Inc. Methods of monitoring disease states and therapies using gene expression profiles
US6232067B1 (en) 1998-08-17 2001-05-15 The Perkin-Elmer Corporation Adapter directed expression analysis
DE69900949T2 (de) 1998-09-18 2002-10-02 Micromet Ag Dns amplifizierung aus einzelnen zellen
EP1124990B1 (de) * 1998-10-27 2006-01-18 Affymetrix, Inc. Komplexitätsmanagement und analyse genomischer dna
US20020012922A1 (en) * 1998-11-04 2002-01-31 Hilbush Brian S. Simplified method for indexing and determining the relative concentration of expressed messenger RNAs
EP1157131A2 (de) * 1999-02-22 2001-11-28 Lynx Therapeutics, Inc. Polymorfische dns fragmente und dessen benutzung
US7008768B1 (en) 1999-02-26 2006-03-07 The United States Of America As Represented By The Department Of Health And Human Services Method for detecting radiation exposure
JP2002540802A (ja) * 1999-04-06 2002-12-03 イェール ユニバーシティ 配列標識の固定されたアドレス分析
WO2001012855A2 (en) * 1999-08-13 2001-02-22 Yale University Binary encoded sequence tags
AU7712400A (en) * 1999-09-23 2001-04-24 Gene Logic, Inc. Indexing populations
CA2397391A1 (en) 2000-01-14 2001-07-19 Integriderm, L.L.C. Informative nucleic arrays and methods for making same
JP2001204463A (ja) * 2000-01-27 2001-07-31 Toyo Kohan Co Ltd ヌクレオチド固定用担体
US6468749B1 (en) 2000-03-30 2002-10-22 Quark Biotech, Inc. Sequence-dependent gene sorting techniques
CA2413423C (en) * 2000-06-30 2011-01-11 Syngenta Participations Ag Method for identification, separation and quantitative measurement of nucleic acid fragments
AU2001280008A1 (en) * 2000-07-21 2002-02-05 Global Genomics Ab A method and an algorithm for mRNA expression analysis
AU2001285155A1 (en) * 2000-08-21 2002-03-04 Lynx Therapeutics, Inc. Polymorphic dna fragments and uses thereof
EP1334113A4 (de) * 2000-10-20 2007-08-08 Expression Diagnostics Inc Erstellen von leukozytenexpressionsprofilen
US7211414B2 (en) * 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
JP4437171B2 (ja) * 2000-12-12 2010-03-24 独立行政法人放射線医学総合研究所 遺伝子の発現を解析する方法
WO2002059357A2 (en) * 2001-01-24 2002-08-01 Genomic Expression Aps Assay and kit for analyzing gene expression
US7026121B1 (en) * 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
US20030170700A1 (en) * 2002-01-09 2003-09-11 Lynx Therapeutics, Inc. Secreted and cell surface polypeptides affected by cholesterol and uses thereof
US20030166026A1 (en) * 2002-01-09 2003-09-04 Lynx Therapeutics, Inc. Identification of specific biomarkers for breast cancer cells
CA2474860A1 (en) * 2002-01-29 2003-08-07 Sten Linnarsson Methods for identifying polyadenylation sites and genes thereof
US20030190618A1 (en) * 2002-03-06 2003-10-09 Babru Samal Method for generating five prime biased tandem tag libraries of cDNAs
US20040224330A1 (en) * 2003-01-15 2004-11-11 Liyan He Nucleic acid indexing
ES2396245T3 (es) 2003-01-29 2013-02-20 454 Life Sciences Corporation Método de amplificación y secuenciamiento de ácidos nucleicos
FR2853907B1 (fr) * 2003-04-18 2007-08-03 Commissariat Energie Atomique Procede de preparation de fragments d'adn par fragmentation selective d'acides nucleiques et ses applications
US20060263813A1 (en) * 2005-05-11 2006-11-23 Expression Diagnostics, Inc. Methods of monitoring functional status of transplants using gene panels
US7892745B2 (en) * 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US20070248978A1 (en) * 2006-04-07 2007-10-25 Expression Diagnostics, Inc. Steroid responsive nucleic acid expression and prediction of disease activity
US7645575B2 (en) * 2004-09-08 2010-01-12 Xdx, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
GB0422551D0 (en) * 2004-10-11 2004-11-10 Univ Liverpool Labelling and sequencing of nucleic acids
US20060160102A1 (en) 2005-01-18 2006-07-20 Hossein Fakhrai-Rad Identification of rare alleles by enzymatic enrichment of mismatched heteroduplexes
US20070092886A1 (en) * 2005-03-22 2007-04-26 Raymond Tabibiazar Methods and compositions for diagnosis, monitoring and development of therapeutics for treatment of atherosclerotic disease
CN1908189A (zh) * 2005-08-02 2007-02-07 博奥生物有限公司 体外辅助鉴定肠型胃癌及其分化程度的方法与专用试剂盒
US8137936B2 (en) * 2005-11-29 2012-03-20 Macevicz Stephen C Selected amplification of polynucleotides
US7932029B1 (en) * 2006-01-04 2011-04-26 Si Lok Methods for nucleic acid mapping and identification of fine-structural-variations in nucleic acids and utilities
ES2393758T3 (es) 2006-03-15 2012-12-27 Micronics, Inc. Ensayos integrados de ácidos nucleicos
US7993832B2 (en) * 2006-08-14 2011-08-09 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
US20090002703A1 (en) * 2006-08-16 2009-01-01 Craig Edward Parman Methods and systems for quantifying isobaric labels and peptides
WO2008140484A2 (en) * 2006-11-09 2008-11-20 Xdx, Inc. Methods for diagnosing and monitoring the status of systemic lupus erythematosus
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
US9074244B2 (en) 2008-03-11 2015-07-07 Affymetrix, Inc. Array-based translocation and rearrangement assays
KR101851117B1 (ko) 2010-01-29 2018-04-23 마이크로닉스 인코포레이티드. 샘플-투-앤서 마이크로유체 카트리지
EP3578205A1 (de) 2010-08-06 2019-12-11 ModernaTX, Inc. Pharmazeutische zusammensetzungen enthaltenbearbeitete nukleinsäuren und ihre medizinische verwendung
CA2813466A1 (en) 2010-10-01 2012-04-05 Moderna Therapeutics, Inc. Modified nucleosides, nucleotides, and nucleic acids that disrupt major groove binding partner interactions
CA2831613A1 (en) 2011-03-31 2012-10-04 Moderna Therapeutics, Inc. Delivery and formulation of engineered nucleic acids
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
CN110511939A (zh) 2011-10-03 2019-11-29 现代泰克斯公司 修饰的核苷、核苷酸和核酸及其用途
US10100092B2 (en) 2011-11-03 2018-10-16 Vca, Inc. Compositions and methods to detect various infectious organisms
EP2791160B1 (de) 2011-12-16 2022-03-02 ModernaTX, Inc. Modifizierte mrna zusammensetzungen
EP2800820B1 (de) 2011-12-19 2020-04-08 Valley Health System Verfahren und kits zur erkennung von patienten mit bauchspeicheldrüsenkrebs
EP2833923A4 (de) 2012-04-02 2016-02-24 Moderna Therapeutics Inc Modifizierte polynukleotide zur herstellung von proteinen
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
US9878056B2 (en) 2012-04-02 2018-01-30 Modernatx, Inc. Modified polynucleotides for the production of cosmetic proteins and peptides
EP3339861B8 (de) 2012-06-15 2023-11-01 Genesis Theranostix Korlatolt Felelossegu Tarsasag Biomarkertest zur vorhersage oder früherkennung von präeklampsie
EP3182126A3 (de) 2012-06-15 2017-08-02 Wayne State University Biomarkertest zur vorhersage oder früherkennung von präeklampsie und/oder hellp-syndrom
ES2765573T3 (es) 2012-08-13 2020-06-09 Univ Rockefeller Tratamiento y diagnóstico de melanoma
ES2782825T3 (es) 2012-10-31 2020-09-16 Univ Rockefeller Tratamiento de cáncer de colon metastásico
PT2922554T (pt) 2012-11-26 2022-06-28 Modernatx Inc Arn modificado nas porções terminais
US10518262B2 (en) 2012-12-21 2019-12-31 Perkinelmer Health Sciences, Inc. Low elasticity films for microfluidic use
JP2016509206A (ja) 2012-12-21 2016-03-24 マイクロニクス, インコーポレイテッド 携帯型蛍光検出システムおよびマイクロアッセイカートリッジ
JP6498125B2 (ja) 2012-12-21 2019-04-10 マイクロニクス, インコーポレイテッド 流体回路および関連する製造方法
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
WO2014182844A1 (en) 2013-05-07 2014-11-13 Micronics, Inc. Microfluidic devices and methods for performing serum separation and blood cross-matching
EP2994543B1 (de) 2013-05-07 2018-08-15 Micronics, Inc. Vorrichtung zur herstellung und analyse von nukleinsäuren
WO2014182831A1 (en) 2013-05-07 2014-11-13 Micronics, Inc. Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions
JP2016530294A (ja) 2013-09-03 2016-09-29 モデルナ セラピューティクス インコーポレイテッドModerna Therapeutics,Inc. キメラポリヌクレオチド
WO2015048744A2 (en) 2013-09-30 2015-04-02 Moderna Therapeutics, Inc. Polynucleotides encoding immune modulating polypeptides
SG11201602503TA (en) 2013-10-03 2016-04-28 Moderna Therapeutics Inc Polynucleotides encoding low density lipoprotein receptor
WO2015187612A1 (en) 2014-06-02 2015-12-10 Valley Health System Method and systems for lung cancer diagnosis
US20170204152A1 (en) 2014-07-16 2017-07-20 Moderna Therapeutics, Inc. Chimeric polynucleotides
CA3078981A1 (en) 2017-11-21 2019-05-31 Rgenix, Inc. Polymorphs and uses thereof
EP3660172A1 (de) 2018-11-28 2020-06-03 Bioscreening and Diagnostics LLC Verfahren zur diagnose von traumatischen hirnverletzungen
WO2021119397A1 (en) 2019-12-13 2021-06-17 Rgenix, Inc. Metal salts and uses thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2036946C (en) * 1990-04-06 2001-10-16 Kenneth V. Deugau Indexing linkers
AU672760B2 (en) * 1991-09-24 1996-10-17 Keygene N.V. Selective restriction fragment amplification: a general method for DNA fingerprinting
EP0592626B1 (de) * 1992-03-11 2003-01-29 Dana-Farber Cancer Institute, Inc. Methode um mrna zu klonieren
GB9214873D0 (en) * 1992-07-13 1992-08-26 Medical Res Council Process for categorising nucleotide sequence populations
US5436142A (en) * 1992-11-12 1995-07-25 Cold Spring Harbor Laboratory Methods for producing probes capable of distingushing variant genomic sequences
US5459037A (en) * 1993-11-12 1995-10-17 The Scripps Research Institute Method for simultaneous identification of differentially expressed mRNAs and measurement of relative concentrations

Also Published As

Publication number Publication date
EP0735144B1 (de) 2002-06-05
AU692685B2 (en) 1998-06-11
EP0735144A1 (de) 1996-10-02
US5707807A (en) 1998-01-13
AU5031196A (en) 1996-10-10
DE69621507T2 (de) 2003-01-09

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