DE69720473D1 - Verfahren zur reinigung von nukleinsäuren - Google Patents

Verfahren zur reinigung von nukleinsäuren

Info

Publication number
DE69720473D1
DE69720473D1 DE69720473T DE69720473T DE69720473D1 DE 69720473 D1 DE69720473 D1 DE 69720473D1 DE 69720473 T DE69720473 T DE 69720473T DE 69720473 T DE69720473 T DE 69720473T DE 69720473 D1 DE69720473 D1 DE 69720473D1
Authority
DE
Germany
Prior art keywords
nucleic acids
purifying nucleic
producing
avoids
efficient
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Revoked
Application number
DE69720473T
Other languages
English (en)
Other versions
DE69720473T2 (de
Inventor
B Bussey
Robert Adamson
Alan Atchley
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Urigen Pharmaceuticals Inc
Original Assignee
Megabios Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24775118&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69720473(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Megabios Corp filed Critical Megabios Corp
Application granted granted Critical
Publication of DE69720473D1 publication Critical patent/DE69720473D1/de
Publication of DE69720473T2 publication Critical patent/DE69720473T2/de
Anticipated expiration legal-status Critical
Revoked legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
DE69720473T 1996-08-01 1997-07-31 Verfahren zur reinigung von nukleinsäuren Revoked DE69720473T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US691090 1996-08-01
US08/691,090 US6011148A (en) 1996-08-01 1996-08-01 Methods for purifying nucleic acids
PCT/US1997/013493 WO1998005673A1 (en) 1996-08-01 1997-07-31 Methods for purifying nucleic acids

Publications (2)

Publication Number Publication Date
DE69720473D1 true DE69720473D1 (de) 2003-05-08
DE69720473T2 DE69720473T2 (de) 2004-05-06

Family

ID=24775118

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69720473T Revoked DE69720473T2 (de) 1996-08-01 1997-07-31 Verfahren zur reinigung von nukleinsäuren

Country Status (9)

Country Link
US (1) US6011148A (de)
EP (1) EP0923592B1 (de)
JP (1) JP3492702B2 (de)
AT (1) ATE236183T1 (de)
AU (1) AU717136B2 (de)
CA (1) CA2261150C (de)
DE (1) DE69720473T2 (de)
DK (1) DK0923592T3 (de)
WO (1) WO1998005673A1 (de)

Families Citing this family (45)

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US5747663A (en) * 1994-02-07 1998-05-05 Qiagen Gmbh Process for the depletion or removal of endotoxins
US7026468B2 (en) * 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) * 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
EP0853123A1 (de) * 1997-01-10 1998-07-15 Roche Diagnostics GmbH Reinigung von DNA durch Cross-Flow-Filtration
GB2337261B (en) * 1998-05-15 2002-09-18 Fsm Technologies Ltd Separation of nucleic acids
US6268492B1 (en) * 1998-08-10 2001-07-31 Chiron Corporation Methods for purifying non-chromosomal nucleic acid molecules from cells
ATE264386T1 (de) * 1999-07-23 2004-04-15 Genentech Inc Verfahren zur rnase- und organische lösemitteln- freier reinigung von plasmid-dns durch tangentialfluss-filtration
DE1272627T1 (de) 2000-04-13 2003-05-28 Millipore Corp Verfahren und vorrichtung zur plasmidgewinnung mit hilfe von ultrafiltration
EP1284287A4 (de) * 2000-05-10 2004-10-13 Mitsubishi Pharma Corp Verfahren zur herstellung eines virusvektors
WO2001094574A2 (en) * 2000-06-07 2001-12-13 Amersham Biosciences Corp Method and apparatus for purifying nucleic acids
GB0028361D0 (en) * 2000-11-21 2001-01-03 Glaxo Group Ltd Method of separating extra chromosomal dna from other cellular components
GB0107634D0 (en) * 2001-03-27 2001-05-16 Fermentas Ab Nucleic acid purification
US6406892B1 (en) 2001-05-23 2002-06-18 Bio-Rad Laboratories, Inc. Acetate-free purification of plasmid DNA on hydroxyapatite
US20030211970A1 (en) * 2001-06-01 2003-11-13 Samuel Nochumson Processing of plasmid-containing fluids
GB0127803D0 (en) * 2001-11-20 2002-01-09 Glaxo Group Ltd Processing nucleic acid
EP1359228B1 (de) * 2002-04-23 2013-11-27 Accenture Global Services Limited Nukleinsäure authentifikation mittels detektion des streulichtes
US7314746B2 (en) 2002-09-13 2008-01-01 Valentis, Inc. Apparatus and method for preparative scale purification of nucleic acids
DE60325773D1 (de) * 2002-12-23 2009-02-26 Vical Inc Reinigungsverfahren für plasmid-dna
DK1628749T3 (da) * 2003-05-30 2019-09-16 Vgxi Inc Anordninger og fremgangsmåder til biomateriel produktion
JP2005095003A (ja) * 2003-09-03 2005-04-14 Fuji Photo Film Co Ltd 核酸の分離精製方法
DE602004021192D1 (de) * 2003-10-09 2009-07-02 Epigenomics Ag Verbesserte bisulfitkonversion von dna
US8298758B2 (en) * 2003-12-26 2012-10-30 Prima Meat Packers, Ltd. Method of multiplex microorganism detection
SE0400490D0 (sv) 2004-02-26 2004-02-26 Amersham Biosciences Ab Plasmid purification
US7531308B2 (en) * 2004-04-23 2009-05-12 Sigma-Aldrich Co. Process for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography
KR20070053268A (ko) 2004-08-16 2007-05-23 네이쳐 테크놀로지 코포레이션 플라스미드 디엔에이 제조에 사용되는 이.콜리의 개량된변종
US20060079150A1 (en) * 2004-10-08 2006-04-13 Miva Filoseta Toy for collecting and dispersing toy vehicles
KR20070093992A (ko) * 2004-12-11 2007-09-19 사이토제닉, 인크. 고품질 핵산의 무세포 생합성 및 그것의 사용
WO2007137226A2 (en) * 2006-05-19 2007-11-29 Ams Research Corporation Method and articles for treatment of stress urinary incontinence
US8546127B2 (en) * 2008-06-30 2013-10-01 General Electric Company Bacteria/RNA extraction device
JP5714291B2 (ja) * 2010-10-15 2015-05-07 独立行政法人農業・食品産業技術総合研究機構 抗酸菌ｄｎａの抽出精製法
AU2012236099A1 (en) 2011-03-31 2013-10-03 Moderna Therapeutics, Inc. Delivery and formulation of engineered nucleic acids
KR20150128687A (ko) * 2013-03-14 2015-11-18 샤이어 휴먼 지네틱 테라피즈 인크. 메신저 rna의 정제 방법
LT2970948T (lt) 2013-03-15 2019-03-25 Glaxosmithkline Biologicals Sa Rna gryninimo būdai
WO2014152027A1 (en) 2013-03-15 2014-09-25 Moderna Therapeutics, Inc. Manufacturing methods for production of rna transcripts
WO2014152030A1 (en) 2013-03-15 2014-09-25 Moderna Therapeutics, Inc. Removal of dna fragments in mrna production process
US11377470B2 (en) 2013-03-15 2022-07-05 Modernatx, Inc. Ribonucleic acid purification
AU2015249312B2 (en) * 2014-04-25 2021-07-29 Translate Bio, Inc. Methods for purification of messenger RNA
DE102014207774B4 (de) * 2014-04-25 2015-12-31 Robert Bosch Gmbh Verfahren und Vorrichtung zur Aufreinigung von biologischen Molekülen
WO2016180430A1 (en) 2015-05-08 2016-11-17 Curevac Ag Method for producing rna
PL4108769T3 (pl) 2015-05-29 2024-02-05 CureVac Manufacturing GmbH Sposób wytwarzania i oczyszczania rna obejmujący co najmniej jeden etap filtracji o przepływie stycznym
GB201600287D0 (en) * 2016-01-07 2016-02-24 Fujifilm Diosynth Biotechnologies Uk Ltd Process
GB201600290D0 (en) 2016-01-07 2016-02-24 Fujifilm Diosynth Biotechnologies Uk Ltd Process
US20200318098A1 (en) * 2016-06-24 2020-10-08 Modernatx, Inc. Methods and apparatus for filtration
WO2020041793A1 (en) 2018-08-24 2020-02-27 Translate Bio, Inc. Methods for purification of messenger rna
EP4329923A1 (de) * 2021-04-30 2024-03-06 Hydranautics Konzentration und diafiltration von oligonukleotiden

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3308932A1 (de) * 1983-03-12 1984-09-13 Hoechst Ag, 6230 Frankfurt Verfahren zur abtrennung von ribonukleinsaeuren aus einer loesung, die desoxyribonukleinsaeuren enthaelt
GB8600582D0 (en) * 1986-01-10 1986-02-19 Ca Minister Nat Defence Purifying biological materials
GB8613476D0 (en) * 1986-06-04 1986-07-09 London Hospital Med Coll Method of sequencing nucleic acids
JP2978518B2 (ja) * 1989-12-06 1999-11-15 東ソー株式会社 ファージｄｎａの精製方法
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA

Also Published As

Publication number Publication date
EP0923592B1 (de) 2003-04-02
DE69720473T2 (de) 2004-05-06
ATE236183T1 (de) 2003-04-15
JP3492702B2 (ja) 2004-02-03
WO1998005673A1 (en) 1998-02-12
WO1998005673A8 (en) 1999-12-09
CA2261150C (en) 2004-04-13
EP0923592A1 (de) 1999-06-23
JP2000500028A (ja) 2000-01-11
CA2261150A1 (en) 1998-02-12
AU717136B2 (en) 2000-03-16
US6011148A (en) 2000-01-04
DK0923592T3 (da) 2003-07-21
AU4049097A (en) 1998-02-25

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Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: VALENTIS INC., BURLINGAME, CALIF., US

8332 No legal effect for de
8370 Indication related to discontinuation of the patent is to be deleted
8363 Opposition against the patent
8331 Complete revocation