DE69725881D1 - Verfahren zur expression von heterologen proteinen in hefe - Google Patents
Verfahren zur expression von heterologen proteinen in hefeInfo
- Publication number
- DE69725881D1 DE69725881D1 DE69725881T DE69725881T DE69725881D1 DE 69725881 D1 DE69725881 D1 DE 69725881D1 DE 69725881 T DE69725881 T DE 69725881T DE 69725881 T DE69725881 T DE 69725881T DE 69725881 D1 DE69725881 D1 DE 69725881D1
- Authority
- DE
- Germany
- Prior art keywords
- isoforms
- proteins
- pdgf
- clipped
- methods
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/18—Growth factors; Growth regulators
- A61K38/1858—Platelet-derived growth factor [PDGF]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/0066—Medicaments; Biocides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/49—Platelet-derived growth factor [PDGF]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/65—Insulin-like growth factors (Somatomedins), e.g. IGF-1, IGF-2
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
- A61L2300/414—Growth factors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US3272096P | 1996-12-13 | 1996-12-13 | |
US32720P | 1996-12-13 | ||
PCT/US1997/022647 WO1998026080A1 (en) | 1996-12-13 | 1997-12-12 | Method for expression of heterologous proteins in yeast |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69725881D1 true DE69725881D1 (de) | 2003-12-11 |
DE69725881T2 DE69725881T2 (de) | 2004-09-09 |
Family
ID=21866458
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69725881T Expired - Lifetime DE69725881T2 (de) | 1996-12-13 | 1997-12-12 | Verfahren zur expression von heterologen proteinen in hefe |
DE69738768T Expired - Lifetime DE69738768D1 (de) | 1996-12-13 | 1997-12-12 | Analyse und separation von pdgf-proteinen |
DE69739319T Expired - Lifetime DE69739319D1 (de) | 1996-12-13 | 1997-12-12 | Verfahren zur Expression von PDGF oder IGR Proteinen in Hefe |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69738768T Expired - Lifetime DE69738768D1 (de) | 1996-12-13 | 1997-12-12 | Analyse und separation von pdgf-proteinen |
DE69739319T Expired - Lifetime DE69739319D1 (de) | 1996-12-13 | 1997-12-12 | Verfahren zur Expression von PDGF oder IGR Proteinen in Hefe |
Country Status (9)
Country | Link |
---|---|
US (10) | US6017731A (de) |
EP (4) | EP1935901A1 (de) |
JP (4) | JP4341859B2 (de) |
AT (3) | ATE398139T1 (de) |
AU (2) | AU5465498A (de) |
DE (3) | DE69725881T2 (de) |
ES (1) | ES2306462T3 (de) |
PT (1) | PT948538E (de) |
WO (2) | WO1998026080A1 (de) |
Families Citing this family (62)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE220101T1 (de) | 1983-04-25 | 2002-07-15 | Chiron Corp | Hybrid-dns-synthesis von reifen insulinähnlichen wachstumsfaktoren |
US5811381A (en) * | 1996-10-10 | 1998-09-22 | Mark A. Emalfarb | Cellulase compositions and methods of use |
US7883872B2 (en) * | 1996-10-10 | 2011-02-08 | Dyadic International (Usa), Inc. | Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose |
US6017731A (en) * | 1996-12-13 | 2000-01-25 | Chiron Corporation | Method for expression of heterologous proteins in yeast |
BRPI9914278B1 (pt) * | 1998-10-06 | 2016-03-08 | Dyadic Internat Usa Inc | cepa recombinante de chrysosporium, e, método para produzir um polipeptídeo de interesse |
US20040146918A1 (en) * | 2000-02-18 | 2004-07-29 | Weiner Michael L. | Hybrid nucleic acid assembly |
US7166712B2 (en) * | 2000-07-12 | 2007-01-23 | Philadelphia, Health And Education Corporation | Mammalian MDM2 binding proteins and uses thereof |
US20080014594A1 (en) * | 2003-01-31 | 2008-01-17 | Kevin Hestir | Lung-Expressed Polypeptides |
BRPI0412279A (pt) | 2003-07-02 | 2006-09-19 | Diversa Corp | glucanases, ácidos nucléicos codificando as mesmas e métodos para preparar e aplicar os mesmos |
DE10342794A1 (de) * | 2003-09-16 | 2005-04-21 | Basf Ag | Sekretion von Proteinen aus Hefen |
JP5236189B2 (ja) | 2003-12-05 | 2013-07-17 | チルドレンズ ホスピタル メディカル センター | オリゴ糖組成物および感染症の治療における該組成物の使用 |
AU2005211362B2 (en) * | 2004-02-02 | 2008-03-13 | Ambrx, Inc. | Modified human interferon polypeptides and their uses |
NZ582684A (en) * | 2004-06-18 | 2011-05-27 | Ambrx Inc | Use of an antibody or binding fragment thereof comprising a non naturally encoded amino acid coupled to a linker |
EP1771573A4 (de) * | 2004-07-21 | 2009-02-18 | Ambrx Inc | Nichtnatürliche kodierte aminosäuren verwendende biosynthetische polypeptide |
US7473678B2 (en) * | 2004-10-14 | 2009-01-06 | Biomimetic Therapeutics, Inc. | Platelet-derived growth factor compositions and methods of use thereof |
JP2008525473A (ja) | 2004-12-22 | 2008-07-17 | アンブレツクス・インコーポレイテツド | 修飾されたヒト成長ホルモン |
JP5425398B2 (ja) | 2004-12-22 | 2014-02-26 | アンブレツクス・インコーポレイテツド | 非天然アミノ酸及びポリペプチドを含む組成物、非天然アミノ酸及びポリペプチドに関連する方法並び非天然アミノ酸及びポリペプチドその使用 |
WO2006071840A2 (en) | 2004-12-22 | 2006-07-06 | Ambrx, Inc. | Formulations of human growth hormone comprising a non-naturally encoded amino acid |
JP4990792B2 (ja) * | 2004-12-22 | 2012-08-01 | アンブレツクス・インコーポレイテツド | アミノアシル−tRNAシンテターゼの組成物およびこの使用 |
MX2007007591A (es) * | 2004-12-22 | 2007-07-25 | Ambrx Inc | Metodos para expresion y purificacion de hormona de crecimiento humano recombinante. |
JP2008541769A (ja) * | 2005-06-03 | 2008-11-27 | アンブレツクス・インコーポレイテツド | 改善されたヒトインターフェロン分子及びそれらの使用 |
US20070048793A1 (en) * | 2005-07-12 | 2007-03-01 | Baynes Brian M | Compositions and methods for biocatalytic engineering |
CN103103238B (zh) | 2005-08-18 | 2016-08-10 | Ambrx公司 | 一种在细胞中制造在特定位置处具有所选氨基酸的抗体或抗体片段多肽的方法 |
FR2891149B1 (fr) | 2005-09-26 | 2007-11-30 | Biodex Sarl | Composition pharmaceutique a action cicatrisante comprenant un derive de dextrane soluble et un facteur de croissance derive des plaquettes. |
ATE500215T1 (de) * | 2005-11-08 | 2011-03-15 | Ambrx Inc | Beschleuniger für die modifikation nichtnatürlicher aminosäuren und nichtnatürlicher aminosäurepolypeptide |
PT2339014E (pt) | 2005-11-16 | 2015-10-13 | Ambrx Inc | Métodos e composições compreendendo aminoácidos não-naturais |
NZ571113A (en) * | 2005-11-17 | 2012-02-24 | Biomimetic Therapeutics Inc | Maxillofacial bone augmentation using rhpdgf-bb and a biocompatible matrix |
CN101448512B (zh) * | 2005-12-14 | 2015-11-25 | Ambrx公司 | 含有非天然氨基酸和多肽的组合物、涉及非天然氨基酸和多肽的方法以及非天然氨基酸和多肽的用途 |
EP2311505B1 (de) | 2006-02-09 | 2013-11-06 | BioMimetic Therapeutics, LLC | Zusammensetzungen und Verfahren zur Behandlung von Knochen |
US9161967B2 (en) | 2006-06-30 | 2015-10-20 | Biomimetic Therapeutics, Llc | Compositions and methods for treating the vertebral column |
US9642891B2 (en) | 2006-06-30 | 2017-05-09 | Biomimetic Therapeutics, Llc | Compositions and methods for treating rotator cuff injuries |
JP5451390B2 (ja) * | 2006-09-08 | 2014-03-26 | アンブルックス,インコーポレイテッド | 脊椎動物細胞内におけるサプレッサーtrnaの転写 |
MX2009002523A (es) | 2006-09-08 | 2009-03-20 | Ambrx Inc | Polipeptido de plasma humano modificado o andamios fc y sus usos. |
US9133495B2 (en) | 2006-09-08 | 2015-09-15 | Ambrx, Inc. | Hybrid suppressor tRNA for vertebrate cells |
EP3181157B1 (de) | 2006-11-03 | 2019-08-14 | BioMimetic Therapeutics, LLC | Zusammensetzungen und verfahren für behandlungen von arthrodese |
US8680252B2 (en) | 2006-12-10 | 2014-03-25 | Dyadic International (Usa), Inc. | Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi |
US9862956B2 (en) | 2006-12-10 | 2018-01-09 | Danisco Us Inc. | Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi |
WO2008121563A2 (en) | 2007-03-30 | 2008-10-09 | Ambrx, Inc. | Modified fgf-21 polypeptides and their uses |
US8114630B2 (en) | 2007-05-02 | 2012-02-14 | Ambrx, Inc. | Modified interferon beta polypeptides and their uses |
CA2736661A1 (en) * | 2007-09-07 | 2009-03-12 | Dyadic International, Inc. | Novel fungal enzymes |
US8946148B2 (en) | 2007-11-20 | 2015-02-03 | Ambrx, Inc. | Modified insulin polypeptides and their uses |
US7943573B2 (en) | 2008-02-07 | 2011-05-17 | Biomimetic Therapeutics, Inc. | Methods for treatment of distraction osteogenesis using PDGF |
NZ586947A (en) | 2008-02-08 | 2012-11-30 | Ambrx Inc | Modified leptin polypeptides and their uses |
US10138283B2 (en) | 2008-07-23 | 2018-11-27 | Ambrx, Inc. | Modified bovine G-CSF polypeptides and their uses |
BR122020000059B8 (pt) | 2008-09-09 | 2021-06-22 | Biomimetic Therapeutics Inc | composição que compreende uma matriz biocompatível e um fator de crescimento derivado de plaqueta e kit |
CN102224238B (zh) | 2008-09-26 | 2015-06-10 | Ambrx公司 | 非天然氨基酸复制依赖性微生物和疫苗 |
CN102232085A (zh) | 2008-09-26 | 2011-11-02 | Ambrx公司 | 修饰的动物促红细胞生成素多肽和其用途 |
US8183346B2 (en) * | 2008-12-05 | 2012-05-22 | Eli Lilly And Company | Anti-ferroportin 1 monoclonal antibodies and uses thereof |
NZ600361A (en) | 2009-12-21 | 2014-06-27 | Ambrx Inc | Modified bovine somatotropin polypeptides and their uses |
CN107056929A (zh) | 2009-12-21 | 2017-08-18 | Ambrx 公司 | 经过修饰的猪促生长素多肽和其用途 |
JP6144049B2 (ja) | 2010-02-22 | 2017-06-07 | バイオミメティック セラピューティクス,リミテッド ライアビリティ カンパニー | 腱障害を処置するための血小板由来成長因子組成物及び方法 |
US9567386B2 (en) | 2010-08-17 | 2017-02-14 | Ambrx, Inc. | Therapeutic uses of modified relaxin polypeptides |
SG187736A1 (en) | 2010-08-17 | 2013-03-28 | Ambrx Inc | Modified relaxin polypeptides and their uses |
TWI480288B (zh) | 2010-09-23 | 2015-04-11 | Lilly Co Eli | 牛顆粒細胞群落刺激因子及其變體之調配物 |
CN105085652B (zh) * | 2014-05-16 | 2021-06-22 | 中国人民解放军军事医学科学院生物工程研究所 | 血小板衍生生长因子b突变体、其制备方法及用途 |
EP3206670A4 (de) * | 2014-10-14 | 2018-06-20 | Samuel Lynch | Zusammensetzung zur behandlung von wunden |
TW202124419A (zh) | 2014-10-24 | 2021-07-01 | 美商必治妥美雅史谷比公司 | 經修飾之fgf-21多肽及其用途 |
CN106496302B (zh) * | 2015-09-08 | 2021-12-10 | 三生国健药业(上海)股份有限公司 | 一种用离子交换层析纯化蛋白质的方法 |
CN108239648B (zh) * | 2016-12-26 | 2023-04-07 | 丰益(上海)生物技术研发中心有限公司 | 高效表达米黑根毛霉脂肪酶的方法 |
AU2018219283B2 (en) | 2017-02-08 | 2022-05-19 | Bristol-Myers Squibb Company | Modified relaxin polypeptides comprising a pharmacokinetic enhancer and uses thereof |
CN110590930B (zh) * | 2018-06-13 | 2023-03-31 | 四川好医生攀西药业有限责任公司 | 美洲大蠊生长因子pdgf在制备损伤修复药物中的应用 |
EP3884027A1 (de) * | 2018-11-19 | 2021-09-29 | Basf Se | Leadersequenz für hefe |
Family Cites Families (49)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2359180A (en) * | 1942-08-11 | 1944-09-26 | Gen Motors Corp | Dynamic balancer |
NZ201918A (en) | 1981-09-18 | 1987-04-30 | Genentech Inc | N-terminal methionyl analogues of bovine growth hormone |
US5010003A (en) * | 1983-04-25 | 1991-04-23 | Genentech, Inc. | Use of yeast homologous signals to secrete heterologous proteins |
EP0487116B1 (de) * | 1984-10-12 | 1999-12-29 | ZymoGenetics, Inc. | Von Blutplättchen abstammende biologisch aktive Analoga eines Wachstumsfaktors in eukaryotischen Zellen |
US4769328A (en) * | 1984-10-12 | 1988-09-06 | Zymogenetics Inc. | Expression of biologically active PDGF analogs in yeast |
US4766073A (en) * | 1985-02-25 | 1988-08-23 | Zymogenetics Inc. | Expression of biologically active PDGF analogs in eucaryotic cells |
US4889919A (en) * | 1986-08-13 | 1989-12-26 | Zymogenetics, Inc. | Biologically active PDGF derived A-chain homodimers |
US4801542A (en) * | 1984-10-12 | 1989-01-31 | Zymogenetics, Inc. | Expression of biologically active PDGF analogs in eucaryotic cells |
US5187263A (en) * | 1984-10-12 | 1993-02-16 | Zymogenetics, Inc. | Expression of biologically active PDGE analogs in eucaryotic cells |
US5045633A (en) * | 1985-02-25 | 1991-09-03 | Zymogenetics, Inc. | Expression of biologically active PDGF analogs in eucaryotic cells |
US5516896A (en) * | 1985-02-25 | 1996-05-14 | Zymogenetics, Inc. | Biologically active B-chain homodimers |
JPS6236183A (ja) * | 1985-06-20 | 1987-02-17 | ザ・サルク・インステイチユ−ト・バイオテクノロジ−/インダストリアル・アソシエイツ・インコ−ポレ−テツド | サツカロミセス・セレビシエからのポリペプチドの発現および分泌 |
ATE131532T1 (de) * | 1986-08-13 | 1995-12-15 | Zymogenetics Inc | Expression von biologisch-aktiven pdgf-analogen in eukaryotischen zellen |
ATE88899T1 (de) | 1986-11-14 | 1993-05-15 | Inst Molecular Biology Inc | Wundheilung und knochenregeneration. |
US5019559A (en) | 1986-11-14 | 1991-05-28 | President And Fellows Of Harvard College | Wound healing using PDGF and IGF-II |
US4873192A (en) | 1987-02-17 | 1989-10-10 | The United States Of America As Represented By The Department Of Health And Human Services | Process for site specific mutagenesis without phenotypic selection |
US5219759A (en) | 1987-04-22 | 1993-06-15 | Chiron Corporation | Recombinant DNA encoding PDGF A-chain polypeptide and expression vectors |
CA1340772C (en) * | 1987-12-30 | 1999-09-28 | Patricia Tekamp-Olson | Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences |
US5094941A (en) * | 1987-12-31 | 1992-03-10 | Zymogenetics, Inc. | Monoclonal antibodies to PDGF |
US5422120A (en) | 1988-05-30 | 1995-06-06 | Depotech Corporation | Heterovesicular liposomes |
DK105489D0 (da) * | 1989-03-03 | 1989-03-03 | Novo Nordisk As | Polypeptid |
US5968778A (en) * | 1989-01-12 | 1999-10-19 | Jurgen Hoppe | PDGF-AB, preparation process and pharmaceuticals containing them |
DE3900770A1 (de) * | 1989-01-12 | 1990-07-26 | Juergen Prof Dr Hoppe | Verfahren zur herstellung von pdgf-a und pdgf-aa |
NZ237464A (en) | 1990-03-21 | 1995-02-24 | Depotech Corp | Liposomes with at least two separate chambers encapsulating two separate biologically active substances |
ES2106031T3 (es) | 1990-04-10 | 1997-11-01 | Inst Molecular Biology Inc | Una composicion comprendiendo un factor de crecimiento, su metodo de obtencion y uso. |
US5962219A (en) * | 1990-06-11 | 1999-10-05 | Nexstar Pharmaceuticals, Inc. | Systematic evolution of ligands by exponential enrichment: chemi-selex |
WO1992001716A1 (en) * | 1990-07-23 | 1992-02-06 | Zymogenetics, Inc. | Protease resistant pdgf and methods of use |
DE552178T1 (de) * | 1990-10-12 | 1994-02-03 | Max Planck Gesellschaft | Abgeänderte ribozyme. |
DK300090D0 (da) * | 1990-12-19 | 1990-12-19 | Novo Nordisk As | Fremgangsmaade til fremstilling af leadersekvenser |
DE4216134A1 (de) * | 1991-06-20 | 1992-12-24 | Europ Lab Molekularbiolog | Synthetische katalytische oligonukleotidstrukturen |
WO1993008825A1 (en) | 1991-11-04 | 1993-05-13 | Zymogenetics, Inc. | Pdgf gel formulation |
ATE312188T1 (de) * | 1993-01-22 | 2005-12-15 | Univ Research Corp | Lokalisierung von therapeutischen mitteln |
WO1994023697A1 (en) | 1993-04-22 | 1994-10-27 | Depotech Corporation | Cyclodextrin liposomes encapsulating pharmacologic compounds and methods for their use |
DK82893D0 (da) * | 1993-07-08 | 1993-07-08 | Novo Nordisk As | Peptid |
US5624803A (en) * | 1993-10-14 | 1997-04-29 | The Regents Of The University Of California | In vivo oligonucleotide generator, and methods of testing the binding affinity of triplex forming oligonucleotides derived therefrom |
BR9408072A (pt) | 1993-11-16 | 1997-08-12 | Depotech Corp | Vesículas com liberação controlada de ativos |
US5902880A (en) * | 1994-08-19 | 1999-05-11 | Ribozyme Pharmaceuticals, Inc. | RNA polymerase III-based expression of therapeutic RNAs |
US5627053A (en) * | 1994-03-29 | 1997-05-06 | Ribozyme Pharmaceuticals, Inc. | 2'deoxy-2'-alkylnucleotide containing nucleic acid |
DE69535703T2 (de) * | 1994-04-13 | 2009-02-19 | The Rockefeller University | AAV-vermittelte Zufuhr von DNA an Zellen des Nervensystems |
US5639642A (en) * | 1994-06-16 | 1997-06-17 | Novo Nordisk A/S | Synthetic leader peptide sequences |
IL114160A (en) * | 1994-06-17 | 2006-12-31 | Novo Nordisk As | Dna constructs encoding heterologous proteins and processes for the heterologous protein production in yeast |
US6146886A (en) * | 1994-08-19 | 2000-11-14 | Ribozyme Pharmaceuticals, Inc. | RNA polymerase III-based expression of therapeutic RNAs |
US5716824A (en) * | 1995-04-20 | 1998-02-10 | Ribozyme Pharmaceuticals, Inc. | 2'-O-alkylthioalkyl and 2-C-alkylthioalkyl-containing enzymatic nucleic acids (ribozymes) |
ZA9610456B (en) * | 1995-12-20 | 1997-06-20 | Novo Nordisk As | N-terminally extended proteins expressed in yeast |
US5998203A (en) * | 1996-04-16 | 1999-12-07 | Ribozyme Pharmaceuticals, Inc. | Enzymatic nucleic acids containing 5'-and/or 3'-cap structures |
US6017731A (en) * | 1996-12-13 | 2000-01-25 | Chiron Corporation | Method for expression of heterologous proteins in yeast |
US6248878B1 (en) * | 1996-12-24 | 2001-06-19 | Ribozyme Pharmaceuticals, Inc. | Nucleoside analogs |
US6001311A (en) * | 1997-02-05 | 1999-12-14 | Protogene Laboratories, Inc. | Apparatus for diverse chemical synthesis using two-dimensional array |
US6395713B1 (en) * | 1997-07-23 | 2002-05-28 | Ribozyme Pharmaceuticals, Inc. | Compositions for the delivery of negatively charged molecules |
-
1997
- 1997-12-12 US US08/989,251 patent/US6017731A/en not_active Expired - Fee Related
- 1997-12-12 DE DE69725881T patent/DE69725881T2/de not_active Expired - Lifetime
- 1997-12-12 AT AT97948629T patent/ATE398139T1/de active
- 1997-12-12 US US08/989,250 patent/US6083910A/en not_active Expired - Lifetime
- 1997-12-12 EP EP08006174A patent/EP1935901A1/de not_active Withdrawn
- 1997-12-12 AU AU54654/98A patent/AU5465498A/en not_active Abandoned
- 1997-12-12 EP EP97948629A patent/EP0948538B1/de not_active Expired - Lifetime
- 1997-12-12 ES ES97948629T patent/ES2306462T3/es not_active Expired - Lifetime
- 1997-12-12 AT AT03012465T patent/ATE426030T1/de not_active IP Right Cessation
- 1997-12-12 EP EP97954073A patent/EP0946736B1/de not_active Expired - Lifetime
- 1997-12-12 DE DE69738768T patent/DE69738768D1/de not_active Expired - Lifetime
- 1997-12-12 WO PCT/US1997/022647 patent/WO1998026080A1/en active IP Right Grant
- 1997-12-12 JP JP52692698A patent/JP4341859B2/ja not_active Expired - Fee Related
- 1997-12-12 WO PCT/US1997/022815 patent/WO1998025963A1/en active Application Filing
- 1997-12-12 JP JP52698598A patent/JP4275741B2/ja not_active Expired - Lifetime
- 1997-12-12 AT AT97954073T patent/ATE253120T1/de not_active IP Right Cessation
- 1997-12-12 DE DE69739319T patent/DE69739319D1/de not_active Expired - Lifetime
- 1997-12-12 EP EP03012465A patent/EP1365028B1/de not_active Expired - Lifetime
- 1997-12-12 PT PT97948629T patent/PT948538E/pt unknown
- 1997-12-12 AU AU57945/98A patent/AU5794598A/en not_active Abandoned
-
1999
- 1999-07-01 US US09/340,250 patent/US6083723A/en not_active Expired - Fee Related
-
2000
- 2000-03-17 US US09/528,108 patent/US6312923B1/en not_active Expired - Fee Related
- 2000-04-14 US US09/549,290 patent/US6448382B1/en not_active Expired - Lifetime
-
2001
- 2001-08-02 US US09/921,398 patent/US6706496B2/en not_active Expired - Fee Related
-
2002
- 2002-07-26 US US10/205,693 patent/US7084262B2/en not_active Expired - Fee Related
- 2002-10-25 US US10/280,826 patent/US6897043B2/en not_active Expired - Fee Related
-
2005
- 2005-01-28 US US11/046,099 patent/US7166446B2/en not_active Expired - Fee Related
-
2006
- 2006-07-07 US US11/483,033 patent/US20060252695A1/en not_active Abandoned
-
2007
- 2007-08-09 JP JP2007208669A patent/JP2008031173A/ja not_active Withdrawn
- 2007-12-27 JP JP2007338286A patent/JP2008119008A/ja not_active Withdrawn
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE69725881D1 (de) | Verfahren zur expression von heterologen proteinen in hefe | |
RU95115239A (ru) | Аналог эритропоэтина | |
DE69532492D1 (de) | Verfahren zur Reinigung von rekombinantem menschlichem Serumalbumin | |
DE69331507T2 (de) | Verfahren zur Reinigung von menschlichem, rekombinantem Serumalbumin | |
DE69513751D1 (de) | Reinigung von Alpha-l-proteinaseinhibitor mittels neuen chromatographischen Trennungsbedingungen | |
DK0409472T3 (da) | Knoglemorfogenetisk protein | |
IT8547835A0 (it) | Procedimento di purificazione di proteine ossee | |
BR9406716A (pt) | Molécula de DNA isolada célula hospedeira vetor método para produzir uma proteina (BMP-10) polipeptideo de proteina-10 morfogenética de osso purificada (BMP-10) e molécula de DNA quimérica | |
DE173233T1 (de) | Methode und mittel fuer chromatografie. | |
DE3177260D1 (de) | Fuer plasminogen-aktivator kodierende rekombinante desoxyribonukleinsaeure und verfahren zur herstellung von plasminogen-aktivator-protein daraus. | |
NO920671L (no) | Fremgangsmaate for aktivering av rekombinante proteiner | |
DE3884356D1 (de) | Verfahren zur Herstellung von Fremdprotein in Hefe, Rekombinant DNS, Transformant. | |
DE69326559T2 (de) | Rekombinante methode und wirt zur herstellung von xylitol | |
EE200000152A (et) | Aminoterminaalselt kärbitud MCP-2 vormid, DNA molekulid, ekspressioonivektor, peremeesrakk, rekombinantne meetod proteiini valmistamiseks, proteiini kasutamine ning farmatseutiline kompositsioon | |
FI862152A0 (fi) | Anordning foer bestaemning av graden av konsistens hos en egenskap foer ett omraode i en diskreta bildelement uppdelad bild. | |
DE59712000D1 (de) | Herstellung von Carbonsäurefluoriden | |
DE3761373D1 (de) | Verfahren zur herstellung von fluor enthaltenden alpha,beta-ungesaettigten carbonsaeuren. | |
ES2193349T3 (es) | Metodo para la produccion de rdspa alfa1. | |
DE69533905D1 (de) | Interferon alpha/beta bindendes Protein, seine Herstellung und Anwendung | |
DE68910036T2 (de) | Luciferase-Gen und neue rekombinante DNA sowie Verfahren zur Herstellung von Luciferase. | |
DE69101425T2 (de) | Methode zur Reinigung von rohem glycidyl (Meth)-Acrylat. | |
DE69503956T2 (de) | Verfahren zur Herstellung von optisch aktiver alpha-(Hydroxyphenoxy)-alkancarbonsäure und ihren Derivaten | |
NO970081D0 (no) | Fremgangsmåte for folding av proteiner som rekombinant hirudin eller epidermisk vekstfaktor | |
DE69716106D1 (de) | Trennung von proteinen durch kapillarelektrophorese mit aminosäure enthaltenden puffern | |
KR970070017A (ko) | 재조합 인 과립구 콜로니 자극인자(rhG-CSF)의 정제방법 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8327 | Change in the person/name/address of the patent owner |
Owner name: NOVARTIS VACCINES AND DIAGNOSTICS,INC.(N.D.GES, US |