DE69733247D1 - Humanisiertes grünes-fluoreszenzprotein und methoden - Google Patents

Humanisiertes grünes-fluoreszenzprotein und methoden

Info

Publication number
DE69733247D1
DE69733247D1 DE69733247T DE69733247T DE69733247D1 DE 69733247 D1 DE69733247 D1 DE 69733247D1 DE 69733247 T DE69733247 T DE 69733247T DE 69733247 T DE69733247 T DE 69733247T DE 69733247 D1 DE69733247 D1 DE 69733247D1
Authority
DE
Germany
Prior art keywords
humanized
mammalian cells
methods
green fluorescence
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69733247T
Other languages
English (en)
Other versions
DE69733247T2 (de
Inventor
Sergei Zolotukhin
Nicholas Muzyczka
W Hauswirth
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Florida
University of Florida Research Foundation Inc
Original Assignee
University of Florida
University of Florida Research Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Florida, University of Florida Research Foundation Inc filed Critical University of Florida
Publication of DE69733247D1 publication Critical patent/DE69733247D1/de
Application granted granted Critical
Publication of DE69733247T2 publication Critical patent/DE69733247T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/025—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a parvovirus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus
DE69733247T 1996-01-18 1997-01-17 Humanisiertes grünes-fluoreszenzprotein und methoden Expired - Lifetime DE69733247T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US588201 1996-01-18
US08/588,201 US5874304A (en) 1996-01-18 1996-01-18 Humanized green fluorescent protein genes and methods
PCT/US1997/000755 WO1997026333A1 (en) 1996-01-18 1997-01-17 Humanized green fluorescent protein genes and methods

Publications (2)

Publication Number Publication Date
DE69733247D1 true DE69733247D1 (de) 2005-06-16
DE69733247T2 DE69733247T2 (de) 2006-01-19

Family

ID=24352898

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69733247T Expired - Lifetime DE69733247T2 (de) 1996-01-18 1997-01-17 Humanisiertes grünes-fluoreszenzprotein und methoden

Country Status (9)

Country Link
US (2) US5874304A (de)
EP (1) EP0874903B1 (de)
JP (2) JP4229469B2 (de)
AT (1) ATE295416T1 (de)
AU (1) AU730842B2 (de)
CA (1) CA2243088C (de)
DE (1) DE69733247T2 (de)
NZ (1) NZ330957A (de)
WO (1) WO1997026333A1 (de)

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US5968750A (en) 1999-10-19
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US5874304A (en) 1999-02-23
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