DE69735308D1 - Enzymzusammensetzung für die freisetzung von zellen aus geweben. - Google Patents

Enzymzusammensetzung für die freisetzung von zellen aus geweben.

Info

Publication number
DE69735308D1
DE69735308D1 DE69735308T DE69735308T DE69735308D1 DE 69735308 D1 DE69735308 D1 DE 69735308D1 DE 69735308 T DE69735308 T DE 69735308T DE 69735308 T DE69735308 T DE 69735308T DE 69735308 D1 DE69735308 D1 DE 69735308D1
Authority
DE
Germany
Prior art keywords
cells
release
woven fabrics
enzyme composition
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69735308T
Other languages
English (en)
Other versions
DE69735308T2 (de
Inventor
E Dwulet
E Smith
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics Corp
Original Assignee
Roche Diagnostics Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Corp filed Critical Roche Diagnostics Corp
Application granted granted Critical
Publication of DE69735308D1 publication Critical patent/DE69735308D1/de
Publication of DE69735308T2 publication Critical patent/DE69735308T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
DE69735308T 1996-12-06 1997-12-04 Enzymzusammensetzung für die freisetzung von zellen aus geweben. Expired - Lifetime DE69735308T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/760,893 US5830741A (en) 1996-12-06 1996-12-06 Composition for tissue dissociation containing collagenase I and II from clostridium histolyticum and a neutral protease
US760893 1996-12-06
PCT/US1997/022848 WO1998024889A1 (en) 1996-12-06 1997-12-04 Enzyme composition for tissue dissociation

Publications (2)

Publication Number Publication Date
DE69735308D1 true DE69735308D1 (de) 2006-04-27
DE69735308T2 DE69735308T2 (de) 2006-10-19

Family

ID=25060487

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69735308T Expired - Lifetime DE69735308T2 (de) 1996-12-06 1997-12-04 Enzymzusammensetzung für die freisetzung von zellen aus geweben.

Country Status (8)

Country Link
US (2) US5830741A (de)
EP (1) EP0907723B1 (de)
JP (1) JP3053875B2 (de)
CA (1) CA2241266C (de)
DE (1) DE69735308T2 (de)
ES (1) ES2259194T3 (de)
MX (1) MX9806064A (de)
WO (1) WO1998024889A1 (de)

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US6251604B1 (en) 1999-08-13 2001-06-26 Genopsys, Inc. Random mutagenesis and amplification of nucleic acid
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US7045349B2 (en) * 2001-01-23 2006-05-16 Benedict Daniel J Method of islet isolation using process control
FR2823217A1 (fr) * 2001-04-09 2002-10-11 Oreal Procede d'obtention d'une suspension de cellules individualisees issues d'un equivalent de peau humaine reconstruite et applications
WO2002092794A2 (en) * 2001-05-17 2002-11-21 Isotis N.V. Isolation method of mesenchymal cells
US20030054331A1 (en) * 2001-09-14 2003-03-20 Stemsource, Inc. Preservation of non embryonic cells from non hematopoietic tissues
US20050048036A1 (en) * 2001-12-07 2005-03-03 Hedrick Marc H. Methods of using regenerative cells in the treatment of inherited and acquired disorders of the bone, bone marrow, liver, and other tissues
US7771716B2 (en) * 2001-12-07 2010-08-10 Cytori Therapeutics, Inc. Methods of using regenerative cells in the treatment of musculoskeletal disorders
US20060204556A1 (en) * 2001-12-07 2006-09-14 Cytori Therapeutics, Inc. Cell-loaded prostheses for regenerative intraluminal applications
US8404229B2 (en) * 2001-12-07 2013-03-26 Cytori Therapeutics, Inc. Methods of using adipose derived stem cells to treat acute tubular necrosis
US8105580B2 (en) 2001-12-07 2012-01-31 Cytori Therapeutics, Inc. Methods of using adipose derived stem cells to promote wound healing
EP1921133B1 (de) * 2001-12-07 2015-05-20 Cytori Therapeutics, Inc. System zur Behandlung von Zellen aus Lipoaspirat
US20050095228A1 (en) * 2001-12-07 2005-05-05 Fraser John K. Methods of using regenerative cells in the treatment of peripheral vascular disease and related disorders
US7595043B2 (en) * 2001-12-07 2009-09-29 Cytori Therapeutics, Inc. Method for processing and using adipose-derived stem cells
US20050008626A1 (en) * 2001-12-07 2005-01-13 Fraser John K. Methods of using adipose tissue-derived cells in the treatment of cardiovascular conditions
US7651684B2 (en) * 2001-12-07 2010-01-26 Cytori Therapeutics, Inc. Methods of using adipose tissue-derived cells in augmenting autologous fat transfer
US9597395B2 (en) 2001-12-07 2017-03-21 Cytori Therapeutics, Inc. Methods of using adipose tissue-derived cells in the treatment of cardiovascular conditions
US7514075B2 (en) * 2001-12-07 2009-04-07 Cytori Therapeutics, Inc. Systems and methods for separating and concentrating adipose derived stem cells from tissue
US7585670B2 (en) 2001-12-07 2009-09-08 Cytori Therapeutics, Inc. Automated methods for isolating and using clinically safe adipose derived regenerative cells
WO2003053362A2 (en) * 2001-12-20 2003-07-03 Macropore, Inc. Systems and methods for treating patients with collagen-rich material extracted from adipose tissue
US20030198631A1 (en) * 2002-04-18 2003-10-23 Healthpoint, Ltd. Thermolysin enzymatic wound debrider
WO2004046305A2 (en) * 2002-11-18 2004-06-03 Agency For Science, Technology And Research Method and system for cell and/or nucleic acid molecules isolation
EP2422622B1 (de) 2003-02-20 2017-01-11 Cytori Therapeutics, Inc. Verfahren zur Verwendung von aus Fettgewebe stammenden Zellen bei der Behandlung von Herz-Kreislauf-Leiden
DE10331171A1 (de) * 2003-07-09 2005-01-27 Nordmark Arzneimittel Gmbh & Co. Kg Neutrale Protease (NP) und Produkt unter Verwendung von neutraler Protease für Gewebedissoziation sowie Verfahren zu dessen Herstellung
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US7273756B2 (en) * 2004-10-01 2007-09-25 Isto Technologies, Inc. Method for chondrocyte expansion with phenotype retention
EP1844434A4 (de) * 2005-01-10 2013-11-13 Cytori Therapeutics Inc Einrichtungen und verfahren zum überwachen, verwalten und warten medizinischer einrichtungen
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US20070224184A1 (en) * 2006-02-22 2007-09-27 The Research Foundation Of The State University Of New York Method for treating cellulite
US20070015278A1 (en) * 2005-04-13 2007-01-18 Yuling Li Methods of isolating differentiable cells from placental-associated tissue and uses thereof
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FR2890976B1 (fr) * 2005-09-20 2007-12-14 Univ La Reunion Kit pour preparer une composition comprenant des cellules adipocytaires et procede d'obtention de ladite composition
US7811560B2 (en) * 2006-01-30 2010-10-12 Auxilium Us Holdings, Llc Compositions and methods for treating collagen-mediated diseases
JP2009528841A (ja) * 2006-03-07 2009-08-13 ヒューマサイト 脂肪組織から内皮細胞を抽出する方法
WO2007139551A1 (en) * 2006-05-30 2007-12-06 Cytori Therapeutics, Inc. Systems and methods for manipulation of regenerative cells from adipose tissue
WO2008013863A2 (en) * 2006-07-26 2008-01-31 Cytori Therapeutics, Inc. Generation of adipose tissue and adipocytes
EP1894581A1 (de) * 2006-08-31 2008-03-05 CellCoTec B.V. Regeneration von Knorpelgewebe unter Verwendung eines Chondrozyten enthaltenden Matrixgels
US8323642B2 (en) * 2006-12-13 2012-12-04 Depuy Mitek, Inc. Tissue fusion method using collagenase for repair of soft tissue
US8163549B2 (en) 2006-12-20 2012-04-24 Zimmer Orthobiologics, Inc. Method of obtaining viable small tissue particles and use for tissue repair
US7632340B2 (en) * 2007-03-07 2009-12-15 Hamilton Beach Brands, Inc. Air purifier for removing particles or contaminants from air
CA2684040C (en) 2007-04-12 2016-12-06 Isto Technologies, Inc. Method of forming an implant using a mold that mimics the shape of the tissue defect site and implant formed therefrom
US10071143B1 (en) 2007-05-03 2018-09-11 The Research Foundation For The State University Of New York Methods for non-surgical treatment of carpal tunnel syndrome
US20100159564A1 (en) * 2007-11-30 2010-06-24 Dwulet Francis E Protease resistant recombinant bacterial collagenases
TWI395593B (zh) * 2008-03-06 2013-05-11 Halozyme Inc 可活化的基質降解酵素之活體內暫時性控制
WO2010021993A1 (en) 2008-08-19 2010-02-25 Cytori Therapeutics, Inc. Methods of using adipose tissue-derived cells in the treatment of the lymphatic system and malignant disease
EP2403523A1 (de) * 2009-03-06 2012-01-11 Halozyme, Inc. Temperaturempfindliche mutanten von matrix metalloprotease 1 und ihre verwendungen
WO2010105820A1 (en) * 2009-03-19 2010-09-23 Roche Diagnostics Gmbh Improved blends containing proteases
AU2010242780B2 (en) 2009-05-01 2016-04-21 Puregraft Llc Systems, methods and compositions for optimizing tissue and cell enriched grafts
ES2385239B1 (es) 2010-09-30 2013-06-19 Proteos Biotech S.L.U. Uso de colagenasa g recombinante, colagenasa h recombinante y pz-peptidasa recombinante para el tratamiento de enfermedades que cursan con alteraciones del colágeno.
WO2012124338A1 (ja) 2011-03-16 2012-09-20 国立大学法人東北大学 生体組織分析用プローブ及びその利用法
US10119131B2 (en) 2011-03-16 2018-11-06 Biospecifics Technologies Corp. Compositions and methods for producing clostridial collagenases
CA2970639C (en) 2012-01-12 2022-04-26 Auxilium International Holdings, Inc. Clostridium histolyticum enzymes and methods for the use thereof
BR102012013110A2 (pt) 2012-05-31 2014-05-27 Cristalia Prod Quimicos Farm Meio de cultura para bactérias do gênero clostridium livre de componentes de origem animal e processo para produção de sobrenadante contendo uma ou mais proteases com atividade colagenolítica e gelatinolítica
JP6282270B2 (ja) * 2012-07-09 2018-02-21 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 組み換えにより生成されたパエニバシラス・ポリミキサに由来する中性プロテアーゼ
WO2014066622A2 (en) 2012-10-24 2014-05-01 The Research Foundation For The State University Of New York Use of collagenase to treat glaucoma
US20140178343A1 (en) 2012-12-21 2014-06-26 Jian Q. Yao Supports and methods for promoting integration of cartilage tissue explants
CA3112772A1 (en) * 2013-02-01 2014-08-07 Tohoku University Method for separating liver cells from liver tissue
EP3034091A1 (de) 2013-03-15 2016-06-22 BioSpecifics Technologies Corporation Behandlungsverfahren und produkt für gebärmutterfibrome mit gereinigter kollagenase
ES2641547T3 (es) 2013-09-19 2017-11-10 Cytori Therapeutics, Inc. Métodos de uso de células derivadas de tejido adiposo en el tratamiento del fenómeno de Raynaud asociado con esclerodermia
CN103751102A (zh) 2014-01-15 2014-04-30 上海交通大学 一种胶原酶温敏水凝胶及其制备方法和应用
JPWO2017038473A1 (ja) * 2015-08-28 2018-06-14 天野エンザイム株式会社 エンドトキシン低減サーモリシン
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EP3384287B1 (de) * 2015-12-01 2020-12-23 The Medical Research, Infrastructure, and Health Services Fund of the Tel-Aviv Medical Center Verbesserte zytometrische assays
ITUB20161010A1 (it) * 2016-02-24 2017-08-24 Abiel S R L Metodo di calibrazione di collagenasi
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CN115969318A (zh) 2017-03-01 2023-04-18 恩多风投有限公司 用于评估和治疗橘皮组织的装置和方法
EP3601556A2 (de) * 2017-03-28 2020-02-05 Endo Ventures Limited Verbessertes verfahren zur herstellung von collagenase
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Also Published As

Publication number Publication date
EP0907723A1 (de) 1999-04-14
JPH11504225A (ja) 1999-04-20
CA2241266A1 (en) 1998-06-11
MX9806064A (es) 1998-11-30
EP0907723A4 (de) 2003-02-05
JP3053875B2 (ja) 2000-06-19
EP0907723B1 (de) 2006-02-22
US5830741A (en) 1998-11-03
CA2241266C (en) 2001-11-27
DE69735308T2 (de) 2006-10-19
WO1998024889A1 (en) 1998-06-11
ES2259194T3 (es) 2006-09-16
US5952215A (en) 1999-09-14

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