DE69739110D1 - Verfahren und Zusammensetzungen zur Bestemmung von zellülaren Response-Profile - Google Patents

Verfahren und Zusammensetzungen zur Bestemmung von zellülaren Response-Profile

Info

Publication number
DE69739110D1
DE69739110D1 DE69739110T DE69739110T DE69739110D1 DE 69739110 D1 DE69739110 D1 DE 69739110D1 DE 69739110 T DE69739110 T DE 69739110T DE 69739110 T DE69739110 T DE 69739110T DE 69739110 D1 DE69739110 D1 DE 69739110D1
Authority
DE
Germany
Prior art keywords
cell
methods
cellular response
compositions
response profiles
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69739110T
Other languages
English (en)
Inventor
Michael A Whitney
Paul A Negulescu
Frank Craig
Lora Mere
Gorden J Foulkes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Vertex Pharmaceuticals San Diego LLC
Original Assignee
Vertex Pharmaceuticals San Diego LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vertex Pharmaceuticals San Diego LLC filed Critical Vertex Pharmaceuticals San Diego LLC
Application granted granted Critical
Publication of DE69739110D1 publication Critical patent/DE69739110D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5041—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects involving analysis of members of signalling pathways
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G01N33/505—Cells of the immune system involving T-cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/978—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • G01N2333/986—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides (3.5.2), e.g. beta-lactamase (penicillinase, 3.5.2.6), creatinine amidohydrolase (creatininase, EC 3.5.2.10), N-methylhydantoinase (3.5.2.6)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells
DE69739110T 1996-09-26 1997-09-26 Verfahren und Zusammensetzungen zur Bestemmung von zellülaren Response-Profile Expired - Lifetime DE69739110D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/719,697 US5928888A (en) 1996-09-26 1996-09-26 Methods and compositions for sensitive and rapid, functional identification of genomic polynucleotides and secondary screening capabilities

Publications (1)

Publication Number Publication Date
DE69739110D1 true DE69739110D1 (de) 2008-12-24

Family

ID=24891025

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69717856T Expired - Lifetime DE69717856T2 (de) 1996-09-26 1997-09-26 Methoden und zusammensetzungen zur empfindlichen und schnellen funktionellen identifizierung genomischer polynukleotide und ihre anwendung in zelltests zum auffinden von medikamenten
DE69739110T Expired - Lifetime DE69739110D1 (de) 1996-09-26 1997-09-26 Verfahren und Zusammensetzungen zur Bestemmung von zellülaren Response-Profile

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE69717856T Expired - Lifetime DE69717856T2 (de) 1996-09-26 1997-09-26 Methoden und zusammensetzungen zur empfindlichen und schnellen funktionellen identifizierung genomischer polynukleotide und ihre anwendung in zelltests zum auffinden von medikamenten

Country Status (10)

Country Link
US (1) US5928888A (de)
EP (2) EP1262478B9 (de)
AT (2) ATE414165T1 (de)
AU (1) AU4505797A (de)
CA (2) CA2409901A1 (de)
DE (2) DE69717856T2 (de)
DK (2) DK1262478T3 (de)
ES (2) ES2315327T4 (de)
PT (1) PT1262478E (de)
WO (1) WO1998013353A1 (de)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5955275A (en) 1997-02-14 1999-09-21 Arcaris, Inc. Methods for identifying nucleic acid sequences encoding agents that affect cellular phenotypes
US6207371B1 (en) * 1996-10-04 2001-03-27 Lexicon Genetics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same
US6060240A (en) * 1996-12-13 2000-05-09 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
US6623922B1 (en) 1997-02-14 2003-09-23 Deltagen Proteomics Methods for identifying, characterizing, and evolving cell-type specific CIS regulatory elements
US7853411B2 (en) 1997-02-27 2010-12-14 Cellomics, Inc. System for cell-based screening
US6759206B1 (en) 1997-02-27 2004-07-06 Cellomics, Inc. System for cell-based screening
CA2280997C (en) * 1997-03-11 2013-05-28 Perry B. Hackett Dna-based transposon system for the introduction of nucleic acid into dna of a cell
US6221612B1 (en) 1997-08-01 2001-04-24 Aurora Biosciences Corporation Photon reducing agents for use in fluorescence assays
US6200762B1 (en) * 1997-08-01 2001-03-13 Aurora Biosciences Corporation Photon reducing agents and compositions for fluorescence assays
US6214563B1 (en) * 1997-08-01 2001-04-10 Aurora Biosciences Corporation Photon reducing agents for reducing undesired light emission in assays
US6828099B2 (en) * 1998-02-02 2004-12-07 Odyssey Thera Inc. Protein fragment complementation assay (PCA) for the detection of protein-protein, protein-small molecule and protein nucleic acid interactions based on the E. coli TEM-1 β-Lactamase
US6284461B1 (en) * 1998-04-28 2001-09-04 Aurora Biosciences Corporation Use of inhibitors in reporter assays
US6280934B1 (en) * 1998-05-13 2001-08-28 Millennium Pharmaceuticals, Inc. Assay for agents which alter G-protein coupled receptor activity
US6031094A (en) * 1998-07-23 2000-02-29 The Regents Of The University Of California Beta-lactam substrates and uses thereof
ATE485834T1 (de) 1998-09-08 2010-11-15 Ford Henry Health System Verfahren zum nachweis von bindegewebswachstumsfaktor zur diagnose von nierkrankheiten
US6063578A (en) * 1998-10-22 2000-05-16 Signal Pharmaceuticals, Inc. Dual reporter system and methods of use therefor
US7160682B2 (en) * 1998-11-13 2007-01-09 Regents Of The University Of Minnesota Nucleic acid transfer vector for the introduction of nucleic acid into the DNA of a cell
US6835540B2 (en) * 2001-03-16 2004-12-28 Mendel Biotechnology, Inc. Biosynthetic pathway transcription factors
WO2000063435A2 (en) * 1999-04-15 2000-10-26 Curagen Corporation Method of identifying toxic agents using differential gene expression
AU7133000A (en) * 1999-07-02 2001-01-22 Curagen Corporation Method of identifying toxic agents using differential gene expression
WO2001053481A1 (en) * 2000-01-20 2001-07-26 Cell Therapeutics, Inc. Methods and compositions for identification of genomic polynucleotides which are transcriptionally regulated
AU2001229741A1 (en) 2000-01-24 2001-07-31 The Trustees Of Columbia University In The City Of New York An in vivo screen using chemical inducers of dimerization
US6897020B2 (en) * 2000-03-20 2005-05-24 Newlink Genetics Inc. Methods and compositions for elucidating relative protein expression levels in cells
US20060134629A1 (en) * 2000-03-20 2006-06-22 Link Charles J Methods and compositions for elucidating protein expression profiles in cells
US7029842B2 (en) 2000-04-07 2006-04-18 Novozymes A/S Signal sequence trapping
DE60134400D1 (de) * 2000-04-07 2008-07-24 Novozymes As Einfangen von signalsequenzen
AU2001294120A1 (en) * 2000-09-07 2002-03-22 Owman Invest, Ltd. Chimeric reporter system for cell surface receptor-ligand binding
US20020127557A1 (en) * 2001-03-09 2002-09-12 Ruoying Tan Method for identification of cDNAs encoding signal peptides
CA2441279A1 (en) * 2001-03-16 2002-09-26 Qtl Biosystems, Llc Fluorescent polymer superquenching-based bioassays
JP2004533245A (ja) * 2001-05-04 2004-11-04 ヘルス　リサーチ　インコーポレイテッド 遺伝子発現モディファイヤーを同定するためのハイスループットアッセイ
JP2005500847A (ja) * 2001-08-24 2005-01-13 アドバンスド セル テクノロジー、インコーポレイテッド 分化誘導薬の同定のためのスクリーニング検定及び細胞治療用の分化細胞の調製
WO2003027260A2 (en) * 2001-09-27 2003-04-03 Functional Genetics, Inc. Methods and compositions for generating homozygous mutations
US20030170611A1 (en) * 2002-03-09 2003-09-11 Cardone Michael H. Cell-based screening methods
US6730492B2 (en) * 2002-03-09 2004-05-04 Merrimack Pharmaceuticals, Inc. Cell-based screening methods
US20030170850A1 (en) * 2002-03-09 2003-09-11 Cardone Michael H. Cell-based screening methods
US20050181464A1 (en) * 2002-04-04 2005-08-18 Affinium Pharmaceuticals, Inc. Novel purified polypeptides from bacteria
AU2003231048A1 (en) * 2002-04-22 2003-11-03 Regents Of The University Of Minnesota Transposon system and methods of use
US7083918B2 (en) 2002-04-24 2006-08-01 The Trustees Of Columbia University In The City Of New York Bacterial small-molecule three-hybrid system
EP1581636A4 (de) * 2002-10-16 2006-09-20 Advanced Cell Tech Inc Verfahren zur verwendung von mit dergenfallentechnik (gene trap) behandelten stammzellen zur markierung vonstammzellendifferenzierungswegen sowie herstellung und isolierungdifferenzierter zellen
AU2004206256A1 (en) * 2003-01-16 2004-08-05 Health Research, Inc. Method for comprehensive identification of cell lineage specific genes
US8460864B2 (en) 2003-01-21 2013-06-11 Ptc Therapeutics, Inc. Methods for identifying compounds that modulate untranslated region-dependent gene expression and methods of using same
US9068234B2 (en) * 2003-01-21 2015-06-30 Ptc Therapeutics, Inc. Methods and agents for screening for compounds capable of modulating gene expression
US8426194B2 (en) 2003-01-21 2013-04-23 Ptc Therapeutics, Inc. Methods and agents for screening for compounds capable of modulating VEGF expression
US20040191873A1 (en) * 2003-03-27 2004-09-30 Won-Bin Young Method for high throughput elucidation of transcriptional profiles and genome annotation
SE0302840D0 (sv) * 2003-10-29 2003-10-29 Affibody Ab Detection method
US20070072186A1 (en) * 2003-11-17 2007-03-29 Anuradha Mehta Methods and agents for screening for compounds capable of modulating her2 expression
GB0410105D0 (en) * 2004-05-06 2004-06-09 Amersham Biosciences Uk Ltd Method for characterising compounds
WO2005113791A2 (en) * 2004-05-07 2005-12-01 Johnson & Johnson Product Research And Development Methods of identifying modulators of bacterial type iii protein secretion system
US20060026699A1 (en) * 2004-06-04 2006-02-02 Largaespada David A Methods and compositions for identification of genomic sequences
US20070044343A1 (en) * 2005-08-30 2007-03-01 Harris Rodger M Disposable footwear cover
US8283115B1 (en) 2007-06-20 2012-10-09 Ptc Therapeutics, Inc. Methods of screening for compounds for treating muscular dystrophy using UTRN mRNA translation regulation
US8283116B1 (en) 2007-06-22 2012-10-09 Ptc Therapeutics, Inc. Methods of screening for compounds for treating spinal muscular atrophy using SMN mRNA translation regulation
BR112015013710B1 (pt) * 2012-12-12 2022-08-09 Promega Corporation Métodos in vitro para detectar uma interação entre um agente bioativo e um alvo celular
US20230384290A1 (en) * 2020-10-16 2023-11-30 Biomems Analytics Llc Cellular Response Analysis Method
IL303969A (en) 2021-01-24 2023-08-01 David Forrest Michael ugh

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5071773A (en) * 1986-10-24 1991-12-10 The Salk Institute For Biological Studies Hormone receptor-related bioassays
US5378603A (en) * 1987-03-30 1995-01-03 Board Of Regents, The University Of Texas System Method and composition for identifying substances which activate transcription of the LDL receptor gene
US4935363A (en) * 1987-03-30 1990-06-19 Board Of Regents, The University Of Texas System Sterol regulatory elements
EP0291893A1 (de) * 1987-05-19 1988-11-23 The Du Pont Merck Pharmaceutical Company Stabile menschliche Zellinien, die ein Indikatorgenprodukt unter virusspezifischen genetischen Kontrollen exprimieren
US5266464A (en) * 1988-02-10 1993-11-30 Ict Pharmaceuticals, Inc. Method of screening for protein inhibitors and activators
US4980281A (en) * 1988-02-10 1990-12-25 Housey Gerard M Method of screening for protein inhibitors and activators
US5264354A (en) * 1988-09-30 1993-11-23 Eli Lilly And Company Method for isolating transposable elements from streptomyces
JPH04503306A (ja) * 1989-02-01 1992-06-18 ザ・ジェネラル・ホスピタル・コーポレーション 単純ヘルペスウイルス１型発現ベクター
US5580722A (en) * 1989-07-18 1996-12-03 Oncogene Science, Inc. Methods of determining chemicals that modulate transcriptionally expression of genes associated with cardiovascular disease
US5665543A (en) * 1989-07-18 1997-09-09 Oncogene Science, Inc. Method of discovering chemicals capable of functioning as gene expression modulators
DE69034061D1 (de) * 1989-07-18 2003-05-28 Osi Pharm Inc Methode der veränderung der expression von genen bei der transkription und zum nachweis von chemischen substanzen, die wie modulatoren der genexpression wirken
US5464764A (en) * 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US5364783A (en) * 1990-05-14 1994-11-15 Massachusetts Institute Of Technology Retrovirus promoter-trap vectors
US5614396A (en) * 1990-06-14 1997-03-25 Baylor College Of Medicine Methods for the genetic modification of endogenous genes in animal cells by homologous recombination
US5401629A (en) * 1990-08-07 1995-03-28 The Salk Institute Biotechnology/Industrial Associates, Inc. Assay methods and compositions useful for measuring the transduction of an intracellular signal
CA2092000A1 (en) * 1990-09-24 1992-03-25 Roger Brent Screening assays
CA2072581A1 (en) * 1990-10-25 1992-04-26 Clague Hodgson Method of gene transfer using retrotransposons
WO1993004179A1 (de) * 1991-08-16 1993-03-04 GSF-Forschungszentrum für Umwelt und Gesundheit Plasmid und in-vitro-test zur detektion von tumorpromotoren unter verwendung dieses plasmids
US5326691A (en) * 1991-11-21 1994-07-05 John Hozier Micro-libraries and methods of making and manipulating them methods for generating and analyzing micro-libraries
DE4138621A1 (de) * 1991-11-25 1993-06-17 Boehringer Ingelheim Int Verfahren zum screenen von substanzen mit modulierender wirkung auf einen rezeptorabhaengigen zellulaeren signaluebertragungsweg
US5338843A (en) * 1992-01-30 1994-08-16 Becton, Dickinson And Company Fluorogenic and chromogenic β-lactamase substrates
WO1993025712A1 (en) * 1992-06-15 1993-12-23 The Regents Of The University Of California Screening assay for the identification of immunosuppressive drugs
WO1994001137A1 (en) * 1992-07-06 1994-01-20 Hybritech Incorporated Method for delivery of cytotoxic agents and components thereof
US5652128A (en) * 1993-01-05 1997-07-29 Jarvik; Jonathan Wallace Method for producing tagged genes, transcripts, and proteins
JP3509100B2 (ja) * 1993-01-21 2004-03-22 プレジデント アンド フェローズ オブ ハーバード カレッジ 哺乳動物のストレスプロモーターを利用する化合物の毒性測定方法および診断用キット
CA2157774A1 (en) * 1993-04-07 1994-10-13 Christopher John Marshall Methods for screening of substances for therapeutic activity and yeast for use therein
DK0695361T4 (da) * 1993-04-21 2010-03-22 Univ Edinburgh Ekspression af heterologe gener ifølge en målekspressionsprofil
US5704555A (en) * 1993-08-02 1998-01-06 Illinois Institute Of Technology Single-screw extruder for solid state shear extrusion pulverization and method
GB9317193D0 (en) * 1993-08-18 1993-10-06 Zeneca Ltd Method
US5604090A (en) * 1994-06-06 1997-02-18 Fred Hutchinson Cancer Research Center Method for increasing transduction of cells by adeno-associated virus vectors
US6010861A (en) * 1994-08-03 2000-01-04 Dgi Biotechnologies, Llc Target specific screens and their use for discovering small organic molecular pharmacophores
WO1996005511A1 (en) * 1994-08-12 1996-02-22 Enzymed, Inc. Biocatalytic methods for synthesizing and identifying biologically active compounds
GB9423367D0 (en) * 1994-11-18 1995-01-11 Wellcome Found Enzyme prodrug therapy
US5789653A (en) * 1995-01-10 1998-08-04 University Of Edinburgh Secretory gene trap
US5741657A (en) * 1995-03-20 1998-04-21 The Regents Of The University Of California Fluorogenic substrates for β-lactamase and methods of use
US5569588A (en) * 1995-08-09 1996-10-29 The Regents Of The University Of California Methods for drug screening
US5777888A (en) * 1995-08-09 1998-07-07 Regents Of The University Of California Systems for generating and analyzing stimulus-response output signal matrices
GB9523703D0 (en) * 1995-11-20 1996-01-24 Wellcome Found Enzyme prodrug thearapy

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CA2409901A1 (en) 1998-04-02
DK1262478T3 (da) 2009-02-09
WO1998013353A1 (en) 1998-04-02
EP1262478A2 (de) 2002-12-04
EP0952976A4 (de) 1999-12-01
CA2266978A1 (en) 1998-04-02
EP0952976A1 (de) 1999-11-03
CA2266978C (en) 2005-06-07
ATE414165T1 (de) 2008-11-15
ES2190802T3 (es) 2003-08-16
ATE229508T1 (de) 2002-12-15
ES2315327T3 (es) 2009-04-01
ES2315327T4 (es) 2009-10-21
PT1262478E (pt) 2009-01-20
EP0952976B1 (de) 2002-12-11
DK0952976T3 (da) 2003-04-07
US5928888A (en) 1999-07-27
EP1262478B1 (de) 2008-11-12
DE69717856T2 (de) 2004-02-19
EP1262478A3 (de) 2003-03-26
EP1262478B9 (de) 2009-04-15
AU4505797A (en) 1998-04-17
DE69717856D1 (de) 2003-01-23

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