DE69926057D1 - Didesoxy farbterminatoren - Google Patents

Didesoxy farbterminatoren

Info

Publication number
DE69926057D1
DE69926057D1 DE69926057T DE69926057T DE69926057D1 DE 69926057 D1 DE69926057 D1 DE 69926057D1 DE 69926057 T DE69926057 T DE 69926057T DE 69926057 T DE69926057 T DE 69926057T DE 69926057 D1 DE69926057 D1 DE 69926057D1
Authority
DE
Germany
Prior art keywords
didesoxy
terminators
color
dye
dye terminator
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69926057T
Other languages
English (en)
Other versions
DE69926057T2 (de
Inventor
Shiv Kumar
Satyam Nampalli
F Mcardle
W Fuller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Global Life Sciences Solutions USA LLC
Original Assignee
Amersham Biosciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Biosciences Corp filed Critical Amersham Biosciences Corp
Application granted granted Critical
Publication of DE69926057D1 publication Critical patent/DE69926057D1/de
Publication of DE69926057T2 publication Critical patent/DE69926057T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
DE69926057T 1998-02-04 1999-02-02 Didesoxy farbterminatoren Expired - Lifetime DE69926057T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US1869598A 1998-02-04 1998-02-04
US18695 1998-02-04
PCT/US1999/002104 WO1999040223A1 (en) 1998-02-04 1999-02-02 Dideoxy dye terminators

Publications (2)

Publication Number Publication Date
DE69926057D1 true DE69926057D1 (de) 2005-08-11
DE69926057T2 DE69926057T2 (de) 2006-04-20

Family

ID=21789323

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69926057T Expired - Lifetime DE69926057T2 (de) 1998-02-04 1999-02-02 Didesoxy farbterminatoren

Country Status (8)

Country Link
US (1) US6949635B1 (de)
EP (1) EP1060264B1 (de)
JP (1) JP4306960B2 (de)
AT (1) ATE299190T1 (de)
AU (1) AU2571799A (de)
CA (1) CA2319777C (de)
DE (1) DE69926057T2 (de)
WO (1) WO1999040223A1 (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4306960B2 (ja) * 1998-02-04 2009-08-05 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション ジデオキシ色素ターミネーター
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6358684B1 (en) * 1999-08-27 2002-03-19 Pe Corporation UV excitable fluorescent energy transfer dyes
CA2382063A1 (en) * 1999-09-17 2001-03-22 Shiv Kumar Charge-modified nucleic acid terminators
AU2002213125B2 (en) 2000-10-11 2007-11-22 Applied Biosystems, Llc. Fluorescent nucleobase conjugates having anionic linkers
JP4676654B2 (ja) * 2001-07-19 2011-04-27 富士フイルム株式会社 蛍光ヌクレオチド及びそれを用いた標識法
JP2007524833A (ja) * 2003-07-01 2007-08-30 ザ リージェンツ オブ ザ ユニバーシティ オブ ミシガン 骨組織の状態を診断するための方法および装置
US20050119587A1 (en) * 2003-07-01 2005-06-02 University Of Michigan Method and apparatus for evaluating connective tissue conditions
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
EP2248911A1 (de) 2004-02-19 2010-11-10 Helicos Biosciences Corporation Verfahren und Zusammensetzungen zur Analyse von Polynukleotidsequenzen
US8227621B2 (en) * 2005-06-30 2012-07-24 Li-Cor, Inc. Cyanine dyes and methods of use
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7729749B2 (en) * 2005-09-01 2010-06-01 The Regents Of The University Of Michigan Method and apparatus for evaluating connective tissue conditions
JP2009508571A (ja) * 2005-09-16 2009-03-05 ザ リージェンツ オブ ザ ユニバーシティ オブ ミシガン 検体の表面直下の組成を計測する方法及びシステム
US20090246762A1 (en) * 2008-03-31 2009-10-01 Applera Corporation, Applied Biosystems Group Nucleic acid terminators incorporating a cationic moiety and methods for their use
CN102459469A (zh) * 2009-04-17 2012-05-16 利康股份有限公司 用取代的花青染料进行荧光成像
WO2012054749A1 (en) 2010-10-20 2012-04-26 Li-Cor, Inc. Cyanine dyes and their conjugates
WO2013041117A1 (en) 2011-09-23 2013-03-28 Illumina Cambrigde Ltd. Dyes for labelling molecular ligands
US8809551B1 (en) 2013-03-08 2014-08-19 Illumina Cambridge Limited Polymethine compounds and their use as fluorescent labels
GB201408077D0 (en) 2014-05-07 2014-06-18 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels
GB201508858D0 (en) 2015-05-22 2015-07-01 Illumina Cambridge Ltd Polymethine compounds with long stokes shifts and their use as fluorescent labels
GB201516987D0 (en) 2015-09-25 2015-11-11 Illumina Cambridge Ltd Polymethine compounds and their use as fluorescent labels

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1340806C (en) 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US5242796A (en) 1986-07-02 1993-09-07 E. I. Du Pont De Nemours And Company Method, system and reagents for DNA sequencing
US5795762A (en) * 1986-08-22 1998-08-18 Roche Molecular Systems, Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5366860A (en) 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5187085A (en) 1990-09-28 1993-02-16 Applied Biosystems, Inc. Nucleic acid sequence analysis with nucleoside-5'-o-(1-thiotriphosphates)
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5986086A (en) * 1997-06-20 1999-11-16 Amersham Pharmacia Biotech Inc. Non-sulfonated cyanine dyes for labeling nucleosides and nucleotides
JP4306960B2 (ja) * 1998-02-04 2009-08-05 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション ジデオキシ色素ターミネーター

Also Published As

Publication number Publication date
DE69926057T2 (de) 2006-04-20
US6949635B1 (en) 2005-09-27
WO1999040223A1 (en) 1999-08-12
EP1060264B1 (de) 2005-07-06
AU2571799A (en) 1999-08-23
EP1060264A4 (de) 2001-07-04
CA2319777A1 (en) 1999-08-12
EP1060264A1 (de) 2000-12-20
ATE299190T1 (de) 2005-07-15
CA2319777C (en) 2008-01-08
JP2002505853A (ja) 2002-02-26
JP4306960B2 (ja) 2009-08-05

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Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: GE HEALTHCARE BIO-SCIENCES CORP., PISCATAWAY, N.J.

8364 No opposition during term of opposition
8328 Change in the person/name/address of the agent

Representative=s name: HAMMONDS LLP, LONDON, GB

R082 Change of representative

Ref document number: 1060264

Country of ref document: EP

Representative=s name: J D REYNOLDS & CO., GB