DE69932813D1 - Nukleinsäurebindungsproteine - Google Patents

Nukleinsäurebindungsproteine

Info

Publication number
DE69932813D1
DE69932813D1 DE69932813T DE69932813T DE69932813D1 DE 69932813 D1 DE69932813 D1 DE 69932813D1 DE 69932813 T DE69932813 T DE 69932813T DE 69932813 T DE69932813 T DE 69932813T DE 69932813 D1 DE69932813 D1 DE 69932813D1
Authority
DE
Germany
Prior art keywords
binding protein
nucleic binding
sequence containing
nucleic
base
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69932813T
Other languages
English (en)
Inventor
Yen Choo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gendaq Ltd
Original Assignee
Gendaq Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9805576.7A external-priority patent/GB9805576D0/en
Priority claimed from GBGB9806895.0A external-priority patent/GB9806895D0/en
Priority claimed from GBGB9807246.5A external-priority patent/GB9807246D0/en
Application filed by Gendaq Ltd filed Critical Gendaq Ltd
Application granted granted Critical
Publication of DE69932813D1 publication Critical patent/DE69932813D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
DE69932813T 1998-03-17 1999-03-17 Nukleinsäurebindungsproteine Expired - Lifetime DE69932813D1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB9805576.7A GB9805576D0 (en) 1998-03-17 1998-03-17 Proteins
GBGB9806895.0A GB9806895D0 (en) 1998-03-31 1998-03-31 Nucleic acid binding proteins
GBGB9807246.5A GB9807246D0 (en) 1998-04-03 1998-04-03 Nucleic acid binding proteins
PCT/GB1999/000816 WO1999047656A2 (en) 1998-03-17 1999-03-17 Nucleic acid binding proteins

Publications (1)

Publication Number Publication Date
DE69932813D1 true DE69932813D1 (de) 2006-09-28

Family

ID=27269244

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69932813T Expired - Lifetime DE69932813D1 (de) 1998-03-17 1999-03-17 Nukleinsäurebindungsproteine

Country Status (9)

Country Link
US (1) US6977154B1 (de)
EP (1) EP1064369B1 (de)
JP (1) JP2002506640A (de)
AT (1) ATE336578T1 (de)
AU (1) AU751487B2 (de)
CA (1) CA2323064C (de)
DE (1) DE69932813D1 (de)
NZ (1) NZ506987A (de)
WO (1) WO1999047656A2 (de)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9824544D0 (en) * 1998-11-09 1999-01-06 Medical Res Council Screening system
US7285416B2 (en) 2000-01-24 2007-10-23 Gendaq Limited Regulated gene expression in plants
EP2022856B1 (de) 1994-08-20 2011-09-14 Gendaq Limited Verbesserungen bei oder im Zusammenhang mit Bindeproteinen zur Erkennung von DNA
US7262055B2 (en) 1998-08-25 2007-08-28 Gendaq Limited Regulated gene expression in plants
GB9710809D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
US6410248B1 (en) 1998-01-30 2002-06-25 Massachusetts Institute Of Technology General strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites
ATE466952T1 (de) 1998-03-02 2010-05-15 Massachusetts Inst Technology Poly-zinkfinger-proteine mit verbesserten linkern
AU751487B2 (en) 1998-03-17 2002-08-15 Gendaq Limited Nucleic acid binding proteins
US6599692B1 (en) 1999-09-14 2003-07-29 Sangamo Bioscience, Inc. Functional genomics using zinc finger proteins
US6453242B1 (en) 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
US7013219B2 (en) 1999-01-12 2006-03-14 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US7070934B2 (en) 1999-01-12 2006-07-04 Sangamo Biosciences, Inc. Ligand-controlled regulation of endogenous gene expression
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US6794136B1 (en) 2000-11-20 2004-09-21 Sangamo Biosciences, Inc. Iterative optimization in the design of binding proteins
US20030104526A1 (en) * 1999-03-24 2003-06-05 Qiang Liu Position dependent recognition of GNN nucleotide triplets by zinc fingers
US7030215B2 (en) 1999-03-24 2006-04-18 Sangamo Biosciences, Inc. Position dependent recognition of GNN nucleotide triplets by zinc fingers
DE60023936T2 (de) 1999-12-06 2006-05-24 Sangamo Biosciences Inc., Richmond Methoden zur verwendung von randomisierten zinkfingerprotein-bibliotheken zur identifizierung von genfunktionen
AU2001226935B2 (en) 2000-01-24 2006-06-22 Gendaq Limited Nucleic acid binding polypeptides characterized by flexible linkers connected nucleic acid binding modules
CN1307027A (zh) * 2000-01-26 2001-08-08 上海博道基因技术有限公司 一种新的多肽——人含tfiis特征性序列片段的锌指蛋白43和编码这种多肽的多核苷酸
EP1254369B1 (de) * 2000-02-08 2010-10-06 Sangamo BioSciences, Inc. Zellen zur entdeckung von medikamenten
ATE374249T1 (de) 2000-04-28 2007-10-15 Sangamo Biosciences Inc Datenbanken von regulatorischen sequenzen, methoden zu ihrer herstellung und verwendung
US7923542B2 (en) 2000-04-28 2011-04-12 Sangamo Biosciences, Inc. Libraries of regulatory sequences, methods of making and using same
WO2001084148A2 (en) 2000-04-28 2001-11-08 Sangamo Biosciences, Inc. Pharmacogenomics and identification of drug targets by reconstruction of signal transduction pathways based on sequences of accessible regions
US6511808B2 (en) 2000-04-28 2003-01-28 Sangamo Biosciences, Inc. Methods for designing exogenous regulatory molecules
US7011972B2 (en) 2000-07-18 2006-03-14 The Scripps Research Institute Fusion polypeptide comprising two ligand binding domains
EP1303606B1 (de) * 2000-07-18 2008-01-02 Novartis AG Regulation der genexpression unter verwendung von einkettigen, monomeren, liganden-abhängigen polypeptidschaltern
IL154059A0 (en) * 2000-07-21 2003-07-31 Syngenta Participations Ag Zinc finger domain recognition code and uses thereof
US7067317B2 (en) 2000-12-07 2006-06-27 Sangamo Biosciences, Inc. Regulation of angiogenesis with zinc finger proteins
AU2884102A (en) 2000-12-07 2002-06-18 Sangamo Biosciences Inc Regulation of angiogenesis with zinc finger proteins
DK1353941T3 (da) 2001-01-22 2013-06-17 Sangamo Biosciences Inc Modificerede zinkfingerbindingsproteiner
WO2002057308A2 (en) * 2001-01-22 2002-07-25 Sangamo Biosciences, Inc. Zinc finger polypeptides and their use
DE10146129A1 (de) * 2001-09-19 2003-04-03 Ina Schaeffler Kg Schaltelement für einen Ventiltrieb einer Brennkraftmaschine
US7262054B2 (en) 2002-01-22 2007-08-28 Sangamo Biosciences, Inc. Zinc finger proteins for DNA binding and gene regulation in plants
US7361635B2 (en) 2002-08-29 2008-04-22 Sangamo Biosciences, Inc. Simultaneous modulation of multiple genes
US8399218B2 (en) * 2007-09-27 2013-03-19 Dow Agrosciences, Llc Engineered zinc finger proteins targeting 5-enolpyruvyl shikimate-3-phosphate synthase genes
DE102008057830A1 (de) 2007-11-21 2009-05-28 Schaeffler Kg Abschaltbarer Stößel
EP2130836B1 (de) * 2008-06-03 2013-02-13 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Mittel und Verfahren zur Herstellung von Zinkfingern und Konkatemere daraus
MY160435A (en) * 2008-06-12 2017-03-15 Univ Putra Malaysia A novel antiviral peptide against avian influenza virus h9n2
WO2011046214A1 (ja) * 2009-10-16 2011-04-21 独立行政法人理化学研究所 メチル化dna結合ペプチド
EP3917571A4 (de) 2019-01-31 2022-10-12 Agency For Science, Technology And Research Cnx/erp57-inhibitor zur verwendung bei der behandlung oder prävention von krebs

Family Cites Families (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3410650A1 (de) 1984-03-23 1985-10-03 Kernforschungsanlage Jülich GmbH, 5170 Jülich Mit mikroorganismen bewachsene poroese anorganische traeger, verfahren zur immobilisierung von mikroorganismen und dafuer geeignete traegerkoerper
US5455170A (en) 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
US5317090A (en) 1987-12-16 1994-05-31 Institut Pasteur Steroid/thyroid hormone receptor-related gene, which is inappropriately expressed in human hepatocellular carcinoma, and which is a retinoic acid receptor
US5324819A (en) 1988-04-08 1994-06-28 Stryker Corporation Osteogenic proteins
US5340739A (en) 1988-07-13 1994-08-23 Brigham & Women's Hospital Hematopoietic cell specific transcriptional regulatory elements of serglycin and uses thereof
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
JPH04504654A (ja) 1988-12-23 1992-08-20 ザ　ソーク　インスティテュート　フォア　バイオロジカル　スタディーズ レセプターの転写抑制活性組成物および方法
US5096815A (en) 1989-01-06 1992-03-17 Protein Engineering Corporation Generation and selection of novel dna-binding proteins and polypeptides
US5198346A (en) 1989-01-06 1993-03-30 Protein Engineering Corp. Generation and selection of novel DNA-binding proteins and polypeptides
US4990607A (en) 1989-03-14 1991-02-05 The Rockefeller University Alteration of gene expression in plants
AU655912B2 (en) 1989-03-17 1995-01-19 Salk Institute For Biological Studies, The Hormone response element compositions and assay
WO1991007509A1 (en) 1989-11-13 1991-05-30 Massachusetts Institute Of Technology Localization and characterization of the wilms's tumor gene
US5578482A (en) 1990-05-25 1996-11-26 Georgetown University Ligand growth factors that bind to the erbB-2 receptor protein and induce cellular responses
DK0552202T3 (da) 1990-09-21 1996-06-03 Salk Inst For Biological Studi Fremgangsmåder medieret af det protoonkogene proteinkompleks AP-1
US5348864A (en) 1991-01-25 1994-09-20 E. R. Squibb & Sons, Inc. Mouse vav proto-oncogene DNA and protein sequences
US5871907A (en) 1991-05-15 1999-02-16 Medical Research Council Methods for producing members of specific binding pairs
US5324818A (en) 1991-08-21 1994-06-28 The Regents Of The University Of Michigan Proteins useful in the regulation of κB-containing genes
US5243041A (en) 1991-08-22 1993-09-07 Fernandez Pol Jose A DNA vector with isolated CDNA gene encoding metallopanstimulin
US5302519A (en) 1991-09-09 1994-04-12 Fred Hutchinson Cancer Research Center Method of producing a Mad polypeptide
US5270170A (en) 1991-10-16 1993-12-14 Affymax Technologies N.V. Peptide library and screening method
US5487994A (en) 1992-04-03 1996-01-30 The Johns Hopkins University Insertion and deletion mutants of FokI restriction endonuclease
US5792640A (en) 1992-04-03 1998-08-11 The Johns Hopkins University General method to clone hybrid restriction endonucleases using lig gene
US5436150A (en) 1992-04-03 1995-07-25 The Johns Hopkins University Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
US5356802A (en) 1992-04-03 1994-10-18 The Johns Hopkins University Functional domains in flavobacterium okeanokoites (FokI) restriction endonuclease
US5916794A (en) 1992-04-03 1999-06-29 Johns Hopkins University Methods for inactivating target DNA and for detecting conformational change in a nucleic acid
US5324638A (en) 1992-05-13 1994-06-28 Sloan-Kettering Institute For Cancer Research Brain transcription factor, nucleic acids encoding same and uses thereof
US6140466A (en) 1994-01-18 2000-10-31 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
WO1995019431A1 (en) 1994-01-18 1995-07-20 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
WO1996006110A1 (en) 1994-08-18 1996-02-29 Gilman, Michael, Z. Composite dna-binding proteins and materials and methods relating thereto
GB9824544D0 (en) 1998-11-09 1999-01-06 Medical Res Council Screening system
EP2022856B1 (de) 1994-08-20 2011-09-14 Gendaq Limited Verbesserungen bei oder im Zusammenhang mit Bindeproteinen zur Erkennung von DNA
DE4435919C1 (de) 1994-10-07 1995-12-07 Deutsches Krebsforsch Zinkfinger-DNA, -Protein und ihre Verwendung
US5871902A (en) 1994-12-09 1999-02-16 The Gene Pool, Inc. Sequence-specific detection of nucleic acid hybrids using a DNA-binding molecule or assembly capable of discriminating perfect hybrids from non-perfect hybrids
US5702914A (en) 1994-12-21 1997-12-30 The Salk Institute For Biological Studies Use of reporter genes for retinoid receptor screening assays having novel retinoid-associated response elements
CA2209183A1 (en) 1994-12-29 1996-07-11 Joel L. Pomerantz Chimeric dna-binding proteins
US5789538A (en) 1995-02-03 1998-08-04 Massachusetts Institute Of Technology Zinc finger proteins with high affinity new DNA binding specificities
AU5445296A (en) 1995-04-12 1996-10-30 Cheng Cheng Methods for preparing dna-binding proteins
EP1295952A3 (de) 1996-01-23 2003-07-09 The Board of Trustees of The Leland Stanford Junior University Verfahren zum screenen für transdominate Effectorpeptiden und RNS molekülen
FR2752734B1 (fr) 1996-09-02 1998-11-06 Cird Galderma Utilisation de retinoides pour la preparation d'un medicament destine a traiter les affections liees a une surexpression de vegf
US5939538A (en) 1996-10-25 1999-08-17 Immusol Incorporated Methods and compositions for inhibiting HIV infection of cells by cleaving HIV co-receptor RNA
GB9710809D0 (en) * 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
GB9710807D0 (en) * 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
JP2002508971A (ja) 1998-01-15 2002-03-26 アリアド・ジーン・セラピューティクス・インコーポレーテッド 多量体キメラ蛋白質を使用する生物学的イベントの調節
US5972615A (en) 1998-01-21 1999-10-26 Urocor, Inc. Biomarkers and targets for diagnosis, prognosis and management of prostate disease
US6410248B1 (en) 1998-01-30 2002-06-25 Massachusetts Institute Of Technology General strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites
DE69933856D1 (de) 1998-02-13 2006-12-14 Koester Hubert Verwendung von ribozymen zur bestimmung der funktion von genen
WO1999042474A2 (en) 1998-02-20 1999-08-26 Genome Dynamics, Inc. Method for designing dna-binding proteins of the zinc-finger class
ATE466952T1 (de) 1998-03-02 2010-05-15 Massachusetts Inst Technology Poly-zinkfinger-proteine mit verbesserten linkern
AU751487B2 (en) 1998-03-17 2002-08-15 Gendaq Limited Nucleic acid binding proteins
US6140081A (en) 1998-10-16 2000-10-31 The Scripps Research Institute Zinc finger binding domains for GNN

Also Published As

Publication number Publication date
JP2002506640A (ja) 2002-03-05
WO1999047656A2 (en) 1999-09-23
WO1999047656A3 (en) 1999-11-25
AU2944999A (en) 1999-10-11
AU751487B2 (en) 2002-08-15
EP1064369A2 (de) 2001-01-03
CA2323064A1 (en) 1999-09-23
CA2323064C (en) 2011-05-31
NZ506987A (en) 2003-01-31
US6977154B1 (en) 2005-12-20
ATE336578T1 (de) 2006-09-15
EP1064369B1 (de) 2006-08-16

Similar Documents

Publication Publication Date Title
DE69932813D1 (de) Nukleinsäurebindungsproteine
DE60023936D1 (de) Methoden zur verwendung von randomisierten zinkfingerprotein-bibliotheken zur identifizierung von genfunktionen
IL188094A0 (en) Il-1?? binding antibodies and fragments thereof
DE69939820D1 (de) Internalisierende erbb2 antikörper
WO2003087129A3 (en) Immunogenic peptides, and method of identifying same
DE60139542D1 (de) Methoden zur herstellung humaner monoklonaler antikörper
ATE555202T1 (de) Verfahren zur lieferung von rna-interferenz und verwendungen damit
CY1110877T1 (el) Αλλαγη των συγγενειων συνδεσης me fcrn ή των ημιζωων ορου αντισωματων με μεταλλαξιγενεση
AU2002217010A1 (en) Peptides, the production and use thereof for binding immunoglobulins
WO2004033631A8 (en) Nucleic acid and polypeptide sequences from lawsonia intracellularis and methods of using
DE69535802D1 (de) Genetische veränderungen, die mit lungencarzinomen korrelieren
IL176479A0 (en) Methods and compositions for identifying rna-binding proteins
ATE473292T1 (de) Sicherheitssystem
ATE300619T1 (de) Humane checkpointkinase, hcds1, zusammensetzungen und verfahren
ATE311443T1 (de) Methoden zur durchforstung (screenen) von proteinen
DE69033128D1 (de) Für leberzellkarzinom spezifisches onkogen
ATE223491T1 (de) Verfahren zur reinigung von biomolekül- oder proteinkomplexen
ATE285474T1 (de) Haemophilus adhäsionsproteinen
DK1208213T3 (da) Fremgangsmåde til fremstilling af proteiner i værtsceller under anvendelse af chaperoniner
ES2178203T3 (es) Metodos y reactivos para modular la apoptosis.
DK1123396T3 (da) Minimale promotorer og anvendelser deraf
WO2001040461A3 (de) cDNA-SEQUENZEN VON ZWEI INTERAKTOREN FANCIP4 UND FANCIP5 DER FANCONI-ANÄMIE-PROTEINE DER KOMPLEMENTATIONSGRUPPEN A UND C
ATE408816T1 (de) Verfahren zur bestimmung sekundärer molekülmodifikationen unter verwendung von arrays
WO2002038765A3 (en) Dna encoding bovine immunoglobulin a inducing protein and uses therefor
SE9302856D0 (sv) Method and means for producing a plasmapeotein-binding protein

Legal Events

Date Code Title Description
8332 No legal effect for de