DE76953T1 - Pseudomonas-zusammensetzungen. - Google Patents

Pseudomonas-zusammensetzungen.

Info

Publication number
DE76953T1
DE76953T1 DE198282108857T DE82108857T DE76953T1 DE 76953 T1 DE76953 T1 DE 76953T1 DE 198282108857 T DE198282108857 T DE 198282108857T DE 82108857 T DE82108857 T DE 82108857T DE 76953 T1 DE76953 T1 DE 76953T1
Authority
DE
Germany
Prior art keywords
pseudomonas
bacterium
genetic material
nrrl
halogenated aromatic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE198282108857T
Other languages
English (en)
Inventor
Ronald Henry Ann Arbor Michigan 48104 Olsen
Peter Anthony Sarasota Florida 33577 Vandenbergh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Microlife Genetics Inc
Original Assignee
Microlife Genetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Microlife Genetics Inc filed Critical Microlife Genetics Inc
Publication of DE76953T1 publication Critical patent/DE76953T1/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/26—Processes using, or culture media containing, hydrocarbons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/38—Pseudomonas
    • C12R2001/385—Pseudomonas aeruginosa
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/38—Pseudomonas
    • C12R2001/40—Pseudomonas putida
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S210/00—Liquid purification or separation
    • Y10S210/902—Materials removed
    • Y10S210/908—Organic
    • Y10S210/909—Aromatic compound, e.g. pcb, phenol
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/874—Pseudomonas
    • Y10S435/875—Pseudomonas aeruginosa
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/874—Pseudomonas
    • Y10S435/877—Pseudomonas putida

Claims (2)

Patentansprüche Bakterium der Gattung Pseudomonas, das genetisches Material enthält, das die Nutzung eines halogenierten Kohlenwasserstoffs als einzige Kohlenstoffquelle kontrolliert, wobei das genetische Material aus einem Stamm von Pseudomonas putida oder Pseudomonas sp. NRRL-B-12 - oder NRRL-B-12 539 aus einer Wachstumsumgebung mit langer Assoziierung an eine halogenierte aromatische Verbindung isolierbar ist, indem man die Bakterien zuerst in Minimalmedium mit L-Tryptophan als einziger Kohlenstoffquelle und dann in einem zweiten Minimalmedium in Anwesenheit einer halogenierten aromatischen Verbindung als zweite alleinige Kohlenstoffquelle wachsen läßt. Bakterium nach Anspruch 1, wobei das genetische Material ein Plasmid in Pseudomonas putida NRRL-B-12 537 ist. 3- Ein Bakterium nach Anspruch 1, wobei das genetische Material in Pseudomonas sp. NRRL-B-12 538 enthalten ist. 4. Bakterium nach Anspruch 1, wobei das genetische Material in Pseudomonas sp. NRRL-B-12 539 enthalten ist. 5. Bakterium nach Anspruch 1, wobei das genetische Material in Pseudomonas aeruginosa NRRL-B-12 536 enthalten ist. 6. Bakterium der Gattung Pseudomonas, das genetisches Ma-S terial von Pseudomonas putida enthält und durch das folgende Verfahren isoliert wurde: a) Zur Verfugung stellen einer Probe aus einer Wachstumsumgebung, die halogenierte aromatische Verbindüngen enthält und mit der während eines ausgedehnten Zeitraums Bakterien der Gattung Pseudomonas einschließlich Pseudomonas putida assoziiert waren; b) isolieren von Pseudomonas aus der Probe in einem Minimalmedium, das L-Tryptophan als einzige Kohlenstoffquelle sowie eine Stickstoffquelle und essentielle Minerale enthält; c) reisolieren der L-Tryptohphan-isolierten Stämme von Pseudomonas in einem geschlossenen Raum in Minimalmedium, das. als einzige Kohlenstoffquelle eine halogenierte aromatische Verbindung enthält und die halogenierte aromatische Verbindung als Gas vorliegen kann. γ. Bakterium nach Anspruch 6, wobei der geschlossene Raum im wesentlichen mit dem Gas gesättigt ist. 8. Bakterien nach Anspruch 6, wobei die Wachstumsumgebung der Präparation eine Bodenprobe ist, die halogenierte ^O aromatische Verbindungen enthält., , 9- Bakterium nach Anspruch 6, wobei die Wachstumsumgebung der Präparation eine Bodenprobe aus einem an eine laufende Ölpumpe angrenzenden Bodenbereich ist. 35 10. Bakterium nach Anspruch 6, wobei die halogenierte aromatische Verbindung in der Präparation ein Monochlortoluol oder Dichlortoluol ist. 11. Bakterium nach Anspruch 6, wobei in Pseudomonas ein Plasmid enthalten ist, das für die Verwertung von Chlortoluol codiert.
1.
2. Bakterium nach Anspruch 11, wobei die Pseudomohas-Art Pseudomonas putida ist, die als genetisches Material ein' Plasmid enthält, das für die Verwertung von Chlrotoluol codiert.
13'· Bakterium nach Anspruch 6, wobei die Pseudomonas-Art Pseudomonas aerüginosa ist, die genetisches Material ent hält, das für die Verwertung von Chlortoluol codiert und aus Pseudomonas putida übertragen wurde.
14. Verfahren nach Anspruch 13, wobei die übertragung durch Transformation erfolgt.
15. Bakterium Pseudomonas putida NRRL-B-12 537-16. Bakterium Pseudomonas sp. NRRL-B-12 538.
17- Bakterium Pseudomonas sp. NRRL-B-12 539. 25
18. Bakterium Pseudomonas aerüginosa NRRL-B-12 536.
19- Bakterium der Gattung Pseudomonas, das genetisches Material enthält, das für den Abbau einer halogenxerten aromatischen Verbindung codiert, wobei das genetische Material von einer Pseudomonas-Art abgeleitet wurde, die aus einer Bodenprobe isoliert wurde, die an eine Ölquelle angrenzt und halogenierte aromatische Verbindungen enthält.
DE198282108857T 1981-10-09 1982-09-24 Pseudomonas-zusammensetzungen. Pending DE76953T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/310,090 US4452894A (en) 1981-10-09 1981-10-09 Pseudomonas compositions

Publications (1)

Publication Number Publication Date
DE76953T1 true DE76953T1 (de) 1983-07-21

Family

ID=23200957

Family Applications (1)

Application Number Title Priority Date Filing Date
DE198282108857T Pending DE76953T1 (de) 1981-10-09 1982-09-24 Pseudomonas-zusammensetzungen.

Country Status (4)

Country Link
US (1) US4452894A (de)
EP (1) EP0076953A3 (de)
DE (1) DE76953T1 (de)
ES (2) ES8400769A1 (de)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4511657A (en) * 1982-05-25 1985-04-16 Occidental Chemical Corporation Treatment of obnoxious chemical wastes
US4479936A (en) * 1982-09-27 1984-10-30 Microlife Technics, Inc. Method for protecting the growth of plants employing mutant siderophore producing strains of Pseudomonas Putida
US4753876A (en) * 1984-03-21 1988-06-28 Monsanto Company Marker genes in pseudomonad bacteria
CA1262339A (en) * 1984-08-14 1989-10-17 Stanley A. Sojka Cloning the halo-organic biodegradable gene
US4664805A (en) * 1985-07-23 1987-05-12 Regents Of The University Of California Analog enrichment decontamination process
US4713343A (en) * 1985-08-29 1987-12-15 The United States Of America As Represented By The Administrator Of The U.S. Environmental Protection Agency Biodegradation of halogenated aliphatic hydrocarbons
WO1987007316A1 (en) * 1986-05-29 1987-12-03 Zapadno-Sibirsky Nauchno-Issledovatelsky Geologora Bacterial composition and method for purifying water and soil of oil pollution
EP0251320B1 (de) * 1986-07-03 1993-03-10 Occidental Chemical Corporation Mikroorganismen für die Zersetzung von giftigem Abfall
US4859594A (en) * 1986-10-14 1989-08-22 Louisana State University Board Of Supervisors, Louisana State University Microorganisms for biodegrading toxic chemicals
US4910143A (en) * 1986-12-08 1990-03-20 Microlife Technics, Inc. Bacterial method and compositions for degrading hydrocarbons
JPH0761261B2 (ja) * 1987-03-05 1995-07-05 新技術事業団 新規微生物
EP0281523B1 (de) * 1987-03-05 1993-07-07 Research Development Corporation Of Japan Pseudomonasstamm
JPH0763357B2 (ja) * 1987-03-05 1995-07-12 新技術事業団 新規微生物
US4959315A (en) * 1987-04-30 1990-09-25 The United States Of America As Represented By The Administrator Of The Environmental Protection Agency Biodegradation of chloroethylene compounds
US5071755A (en) * 1987-04-30 1991-12-10 Ecova Corporation Biodegradation of aliphatic chloroethylene compounds
US4855051A (en) * 1987-05-11 1989-08-08 Polysar Limited Microbial treatment of wastewater to remove tertiary butyl alcohol
US5036012A (en) * 1987-11-03 1991-07-30 Wolfram James H Biodegradation of liquid scintillation cocktails
US4863872A (en) * 1987-11-03 1989-09-05 Wolfram James H Novel pseudomonas microorganism for biodegradation of liquid scintillation cocktails
JPH084516B2 (ja) * 1987-11-09 1996-01-24 出光興産株式会社 有機化合物の製造法
JPH084515B2 (ja) * 1987-11-09 1996-01-24 出光興産株式会社 有機化合物の製造方法
US4872986A (en) * 1988-05-06 1989-10-10 Sybron Chemicals, Inc. Use of bacteria for control of algal bloom in wastewater, lagoons, or ponds
US5057221A (en) * 1988-12-19 1991-10-15 Weyerhaeuser Company Aerobic biological dehalogenation reactor
US5037551A (en) * 1988-12-19 1991-08-06 Weyerhaeuser Company High-flow rate capacity aerobic biological dehalogenation reactor
US4925802A (en) * 1988-12-21 1990-05-15 Ecova Corporation Method for stimulating biodegradation of halogenated aliphatic hydrocarbons
CA2066378C (en) * 1991-04-24 2000-09-19 David J. Hardman Dehalogenation of organohalogen-containing compounds
US5935843A (en) * 1992-02-13 1999-08-10 Osprey Biotechnics, Inc. Method and apparatus for waste degradation
US6245552B1 (en) 1992-02-13 2001-06-12 Osprey Biotechnics, Inc. Method for waste degradation
US5364789A (en) * 1994-01-14 1994-11-15 Guinn Lloyd J Microbial cleaner
WO1996011071A2 (en) * 1994-09-30 1996-04-18 Chemfree Corporation Parts washing system
US6328045B1 (en) 1994-09-30 2001-12-11 Chemfree Corporation Parts washing system
US5972691A (en) * 1995-06-07 1999-10-26 Hercules Incorporated Dehalogenation of polyamine, neutral curing wet strength resins
DE19647847B4 (de) * 1995-11-20 2008-11-13 Kabushiki Kaisha Toshiba, Kawasaki Verfahren zum Zersetzen organischer Verbindungen, Vorrichtung zum Zersetzen organischer Verbindungen, Verfahren zum Isolieren von Mikroorganismen der Art Komagatella brevis und neuer Mikroorganismus der Art Komagatella brevis
ES2391785T3 (es) * 1999-06-11 2012-11-29 Hercules Incorporated Proceso para preparar resinas de poliamina-epihalohidrina con contenido reducido de subproductos
CN1293122C (zh) * 2000-12-09 2007-01-03 赫尔克里士公司 副产物降低的高固体聚胺－表卤代醇组合物
US20030070783A1 (en) * 2000-12-09 2003-04-17 Riehle Richard James Reduced byproduct high solids polyamine-epihalohydrin compositions
LT5057B (lt) 2001-12-05 2003-09-25 Viešoji Įstaiga "Grunto Valymo Technologijos" Biopreparatas nafta ir jos produktais užterštam gruntui ir vandeniui valyti, jo gavimo būdas ir panaudojimas
US8187862B2 (en) * 2007-03-06 2012-05-29 CL Solutions Bioremediation methods
US8114659B2 (en) * 2008-01-14 2012-02-14 Robert William Rawson Apparatus and method for catalytic treatment of a media
US8609398B1 (en) 2012-08-14 2013-12-17 Osprey Biotechnics, Inc. Selective biodegradation of free fatty acids in fat-containing waste

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS4946917B1 (de) * 1970-12-22 1974-12-12
JPS5119037B2 (de) * 1972-11-16 1976-06-14
US4259444A (en) * 1972-06-07 1981-03-31 General Electric Company Microorganisms having multiple compatible degradative energy-generating plasmids and preparation thereof
US3923603A (en) * 1974-08-01 1975-12-02 Gen Electric Discrete plasmid construction from chromosomal genes in pseudomonas
SU647339A1 (ru) * 1977-05-31 1979-02-15 Институт Биохимии И Физиологии Микроорганизмов Ан Ссср Штамм
US4477570A (en) * 1981-09-24 1984-10-16 Occidental Chemical Corporation Microbial degradation of obnoxious organic wastes into innocucous materials

Also Published As

Publication number Publication date
EP0076953A3 (de) 1984-07-04
ES520046A0 (es) 1984-04-01
ES516354A0 (es) 1983-11-01
ES8403967A1 (es) 1984-04-01
US4452894A (en) 1984-06-05
ES8400769A1 (es) 1983-11-01
EP0076953A2 (de) 1983-04-20

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