EP0862560A1 - Substituted 2-anilinopyrimidines useful as protein kinase inhibitors - Google Patents

Abstract

Compounds of general formula (1) are described wherein R1 is a hydrogen or halogen atom or an optionally substitued straight or branched chain alkyl, alkenyl or alkynyl group or a group selected from hydroxyl (-OH), substituted hydroxyl, thiol (-SH), substituted thiol, amino (-NH¿2?), or substituted amino; R?2 and R3¿, which may be the same or different, is each an optionally substituted straight or branched chain alkyl, alkenyl or alkynyl group; R4 is a hydrogen atom or a straight or branched chain alkyl group; R5 is a hydrogen atom or an optionally substituted straight or branched chain alkyl, alkenyl or alkynyl group; R6 is a hydrogen or halogen atom or an amino (-NH¿2?), substituted amino, nitro, carboxyl (-CO2H) or esterified carboxyl group or a group -X?1-R6a¿ where X1 is a direct bond or a linker atom or group and R6a is an optionally substituted straight or branched chain alkyl, alkenyl or alkynyl group; X is a direct bond or a linker atom or group; R7 is an optionally substituted aliphatic, cycloaliphatic, heteroaliphatic, heterocycloaliphatic, aromatic or heteroaromatic group; and the salts, solvates, hydrates and N-oxides thereof. The compounds are selective protein kinase inhibitors, particularly the kinases p56?lck, p59fyn¿, ZAP-70 and protein kinase C and are of use in the prophylaxis and treatment of immune diseases, hyperproliferative disorders and other diseases in which inappropriate protein kinase action is believed to have a role.

Description

SUBSTITUTED 2-ANILINOPYRIMIDINES USEFUL AS PROTEIN

KINASE INHIBITORS

This invention relates to substituted 2-anilinopyrimidines, to processes for their preparation, to pharmaceutical compositions containing them, and to their use in medicine.

Protein kinases participate in the signalling events which control the activation, growth and differentiation of cells in response to extracellular mediators and to changes in the environment. In general, these kinases fall into two groups; those which preferentially phosphorylate serine and/or threonine residues and those which preferentially phosphorylate tyrosine residues [Hanks, S K, Hunter T, FASEB. J. 9, 576-596 (1995)]. The serine/threonine kinases include for example, protein kinase C isoforms [Newton A C, J. Biol. Chem. 270, 28495-28498 (1995)] and a group of cyclin-dependent kinases such as cdc2 [Pines J, Trends in Biochemical Sciences 18, 195-197 (1995)]. The tyrosine kinases include membrane-spanning growth factor receptors such as the epidermal growth factor receptor [Iwashita S and Kobayashi M. Cellular Signalling 4, 123- 132 (1992)], and cytosolic non-receptor kinases such as p56^lck p59^fyn ZAP-70 and csk kinases [Chan C et al Ann. Rev. Immunol. 12 , 555-592 (1994)].

Inappropriately high protein kinase activity has been implicated in many diseases resulting from abnormal cellular function. This might arise either directly or indirectly, for example by failure of the proper control mechanisms for the kinase, related for example to mutation, overexpression or inappropriate activation of the enzyme; or by over- or underproduction of cytokines or growth factors also participating in the transduction of signal upstream or downstream of the kinase. In all of these instances, selective inhibition of the action of the kinase might be expected to have a beneficial effect.

We have now found a series of substituted 2-anilinopyhmidines which are potent and selective inhibitors of protein kinases, especially the kinases p56^lck, p59^fvn, ZAP-70 and protein kinase C The compounds are thus of use in the prophylaxis and treatment of immune diseases, hyper- prohferative disorders and other diseases in which inappropriate protein kinase action is believed to have a role.

Thus, according to one aspect of the invention, we provide a compound of formula (1 ):

wherein R¹ is a hydrogen or halogen atom or an optionally substituted straight or branched chain alkyl, alkenyl or alkynyl group or a group selected from hydroxyl (-OH), substituted hydroxyl, thiol (-SH), substituted thiol, am o (-NH2), or substituted ammo,

R² and R³, which may be the same or different, is each an optionally substituted straight or branched chain alkyl, alkenyl or alkynyl group,

R⁴ is a hydrogen atom or a straight or branched chain alkyl group;

R⁵ is a hydrogen atom or an optionally substituted straight or branched chain alkyl, alkenyl or alkynyl group,

R⁶ is a hydrogen or halogen atom or an ammo (-NH2), substituted ammo, nitro, carboxyl (-CO2H) or esterified carboxyl group or a group -X¹ -R^6a where X¹ is a direct bond or a linker atom or group and R^6a is an optionally substituted straight or branched chain alkyl, alkenyl or alkynyl group,

X is a direct bond or a linker atom or group,

R⁷ is an optionally substituted aliphatic, cycloaliphatic, heteroaliphatic, heterocycloa phatic, aromatic or heteroaromatic group; and the salts, solvates, hydrates and N-oxides thereof Halogen atoms represented by the group R¹ and/or R⁶ in compounds of formula (1 ) include for example fluorine, chlorine, bromine or iodine atoms

When R¹ , R², R³, R⁵ and/or R^6a is an optionally substituted straight or branched chain alkyl, alkenyl or alkynyl group each of said groups may independently be an optionally substituted straight or branched chain C-ι-6 alkyl, e.g. C₁-3 alkyl, C2-6 alkenyl, e.g. C_2-4 alkenyl, or C -6 alkynyl, e g C2-₄ alkynyl group. Particular examples of such groups include optionally substituted -CH₃, -CH₂CH₃, -(CH₂)₂CH₃, -CH(CH₃)₂, -(CH₂)₃CH₃, -CH(CH₃)CH₂CH₃, -CH₂CH(CH₃)₂, -C(CH₃)_3l -(CH₂)₄CH₃, -(CH₂)₅CH₃, -CHCH₂, -CHCHCH₃, -CH₂CHCH₂, -CHCHCH2CH3, -CH2CHCHCH3, -(CH₂)2CHCH₂, -CCH, -CCCH3, -CH₂CCH, -CCCH₂CH₃, -CH₂CCCH₃, or -(CH₂)₂CCH groups The optional substituents which may be present on these groups include one, two, three or more substituents selected from halogen atoms, e g fluorine, chlorine, bromine or iodine atoms, or hydroxyl, C1-6 alkoxy, e.g. methoxy or ethoxy, thiol, C1-6 alkylthio e.g methylthio or ethylthio, ammo, C1 -6 alkylamino, e.g methylamino or ethylamino, or C1 -6 dialkylamino, e g dimethylamino or diethylamino groups

Substituted hydroxyl groups represented by the group R¹ in compounds of formula (1 ) include -OR⁸ groups where R⁸ is an optionally substituted straight or branched C₁-6 alkyl, e.g. methyl or ethyl, C₂-₆ alkenyl, e g allyl, or C2-₆ alkynyl, e.g. ethynyl group. The optional substituents which may be present on these groups include a phenyl group and/or one, two, three or more of the atoms or groups described above in relation to substituents present on alkyl groups represented by R¹

Substituted thiol groups represented by the group R¹ include -SR⁸ groups, wherein R⁸ is as just defined.

When R¹ and/or R⁶ is a substituted am o group it may be for example a -NHR⁹ or -NR⁹R¹⁰ group where R⁹ and R¹⁰, which may be the same or different, is each a group -R⁸ or -COR⁸ where R⁸ is as just defined Esterified carboxyl groups represented by the group R⁶ include groups of formula -C0₂Alk¹ wherein -Cθ2Alk¹ is as defined hereinafter in connection with esterified carboxyl groups represented by the group R¹³.

Linker atoms represented by X or X¹ in compounds of formula (1 ) include -0- or -S- atoms. When X or X¹ is a linker group it may be for example a -C(O)-, -C(S)-₍ -S(O)-, -S(0)₂-, -N(RH)- [where RU is a hydrogen atom or a C-₁ -6 alkyi, e.g. methyl or ethyl, group], -CON(R^{1 1} )-, -0C(0)N(R¹1 )-, -CSN(RH )-, -N(R^{1 1} )C0-, -N(R^"" ¹ )C(0)0-, -N(RH )CS-, -SON(RH), -S0₂N(R^{1 1} ), -N(R¹1 )S0₂-, -N(RH )C0N(R1¹ )-, -N(R^{1 1} )CSN ( RH)-, -N(R^{1 1} )SON(R^{1 1})- or -N(R¹ )S0₂N(RH) group.

When R⁷ in compounds of formula (1 ) is an optionally substituted aliphatic or cycloaliphatic group it may be an optionally substituted C-ι-₁₀ aliphatic or C3-₁0 cycloaliphatic group. Particular examples include optionally substituted straight or branched chain C-i-e alkyl, C2-6 alkenyl, or C2-6 alkynyl groups or optionally substituted C_3-ιocycloalkyl, C_3-ιocycloalkenyl or C_3-iocycloalkynyl groups.

Heteroaliphatic or heterocycloaliphatic groups represented by R⁷ include the aliphatic or cycloaliphatic groups just described but with each group additionally containing one, two, three or four heteroatoms or heteroatom- containing groups. Particular heteroatoms or groups include atoms or groups -X²- where X² is as defined above for X when X is a linker atom or group.

Aromatic groups represented by R⁷ in compounds of formula (1 ) include for example optionally substituted monocyclic or bicyclic fused ring C6-₁2 aromatic groups, such as optionally substituted phenyl, 1 - or 2-naphthyl, 1 - or 2-tetrahydronaphthyl, indanyl or indenyl groups.

Heteroaromatic groups represented by R⁷ include optionally substituted C-ι-9 heteroaromatic groups containing for example one, two, three or four heteroatoms selected from oxygen, sulphur or nitrogen atoms. In general, the heteroaromatic groups may be for example monocyclic or bicyclic fused ring heteroaromatic groups. Monocyclic heteroaromatic groups include for example five- or six-membered heteroaromatic groups containing one, two, three or four heteroatoms selected from oxygen, sulphur or nitrogen atoms. Bicyclic heteroaromatic groups include for example nine- to thirteen-membered fused-ring heteroaromatic groups containing one, two or more heteroatoms selected from oxygen, sulphur or nitrogen atoms.

In general, when R⁷ is a heteroaliphatic, heterocycloaliphatic or heteroaromatic group it is attached to the remainder of the molecule of formula (1 ) through any available heteroatom or group or, preferably, carbon atom.

Particular examples of R⁷ aliphatic groups include those alkyl, alkenyl or alkynyl groups specifically described above in relation to the groups R¹ , R², R³, R⁵ and R⁶. Each of these groups may be optionally substituted, and/or optionally interrupted by one or two heteroatoms or heteratom- containing groups represented by -X²- [where X² is as previously defined], to yield particular examples of R⁷ optionally substituted aliphatic or heteroaliphatic groups.

Particular examples of R⁷ cycloaliphatic and heterocycloaliphatic groups include optionally substitued cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycioheptyl, 2-cyclobuten-l -yl, 2-cyclopenten-1 -yl, 3- cyclopenten-1 -yl, 2,4-cyclopentadien-1 -yl, 3,5,-cyclohexadien-1 -yl, pyrroline, e.g. 2- or 3-pyrrolinyl, pyrrolidinyl, dioxolanyl, e.g. 1 ,3-dioxolanyl, imidazolinyl, e.g. 2-imidazolinyl, imidazolidiπyl, pyrazolinyl, e.g. 2- pyrazoiinyl, pyrazolidinyl, pyranyl, e.g. 2- or 4-pyranyl, piperidinyl, 1 ,4- dioxanyl, morpholinyl, 1 ,4-dithianyl, thiomorpholinyl, piperazinyl, 1 ,3,5- trithianyl, oxazinyl, e.g. 2H-1.3-, 6H-1.3-, 6H-1 ,2-, 2H-1.2- or 4H-1 .4- oxazinyl, 1 ,2,5-oxathiazinyl, isoxazinyl, e.g. o- or p-isoxazinyl, oxathiazinyl, e.g. 1 ,2,5 or 1 ,2,6-oxathiazinyl, or 1 ,3,5,2-oxadiazinyl groups.

Examples of heteroaromatic groups represented by R⁷ include optionally substituted pyrrolyl, furyl, thienyl, imidazolyl, N-methylimidazolyl, N-ethyl- imidazolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, pyrazolyl,

1 ,2,3-triazolyl, 1 ,2,4-thazolyl, 1 ,2,3-oxadiazolyl, 1 ,2,4-oxadiazolyl, 1 ,2,5-oxadiazolyl, 1 ,3,4-oxadiazolyl, 1 ,3,4-thiadiazole, pyridyl, pyrimidinyl, pyridazinyl, pyrazinyl, 1 ,3,5-triazinyl, 1 ,2,4-triazinyl, 1 ,2,3-triazinyl, benzofuryl, [2,3-dihydro]benzofuryl, isobenzofuryl, benzothienyl, benzotriazolyl, isobenzothienyl, indolyl, isoindolyl, benzimidazolyl, imidazo[1 ,2-a]pyridyl, benzothiazolyl, benzoxazolyl, benzopyranyl, [3,4- dihydro]benzopyranyl, quinazolinyl, naphthyridinyl, pyrido[3,4-b]pyridyl, pyrido[3,2-b]pyridyl, pyrido[4,3-b]pyridyl, quinolinyl, isoquinolinyl, tetrazolyl, 5,6,7,8-tetrahydroquinolinyl, 5,6,7,8-tetrahydroisoquinolinyl, and imidyl, e.g. succinimidyl, phthalimidyl, or naphthalimidyl such as 1 ,8- naphthalimidyl.

Optional substituents which may be present on any of the above R⁷ groups in compounds of formula (1 ) include one, two, three or more substituents, each represented by the group R^{1 2}. The substituent R^{1 2} may be selected from an atom or group R¹³ or -Alk(R ³)_m, where R¹³ is a halogen atom, or an amino (-NH₂), -NHR¹⁴ [where R¹⁴ is an -Alk(R¹³)_m, heterocycloalkyl, -Alk-heterocycloalkyl, aryl or heteroaryl group], -N(R¹ )₂ [where each R¹⁴ group is the same or different], nitro, cyano, hydroxyl (-OH), -OR¹⁴, formyl, carboxyl (-C0₂H), esterified carboxyl, thiol (-SH), -SR^{1 4}, -COR^{1 4}, -CSR^{1 4} , -S0₃H, -S0₂R^{1 4}, -S0₂ NH₂, -S0₂ NHR¹⁴, S0₂N[R¹⁴]₂, -CONH₂ , -CSNH₂, -CONHR¹⁴, -CSNHR¹⁴, -CON[R¹⁴]₂, -CSN[R¹⁴]₂, -NHS0₂ H, -NHS0₂ R^{1 4} , -N[S0₂ R¹⁴]₂, -NHS0₂ NH₂ -NHS0₂NHR¹⁴, -NHS0₂N[R¹ ]₂, -NHCOR¹⁴, -NHCONH₂, -NHCONHR¹⁴, -NHCON[R¹⁴]₂, -NHCSR¹⁴, -NHCSNH₂, -NHCSNHR¹⁴, -NHCSN[R¹⁴]₂, -NHC(0)OR¹⁴, or an optionally substituted cycloalkyl aryl or heteroaryl group; or R¹² may be an optionally substituted heterocycloalkyl or -Alk- heterocycloalkyl group provided that when R¹² is a heterocycloalkyl group X in compounds of the invention is either a linker atom or group, or X is a bond and R⁷ is an optionally substituted aliphatic, cycloaliphatic, heteroaliphatic, heterocycloaliphatic, aromatic or heteroaromatic group other than a pyridyl group; Alk is a straight or branched C_{1 -6} alkylene, C_2-6 alkenylene or C₂-₆ alkynylene chain, optionally interrupted by one, two or three -O- or -S- atoms or S-(O)-, -S(0)₂- or -N(R ¹ )- groups; and m is zero or an integer 1 , 2 or 3. When in the group -Alk(R^{1 3})_m m is an integer 1 , 2 or 3, it is to be understood that the substituent or substituents R¹³ may be present on any suitable carbon atom in -Alk Where more than one R¹³ substituent is present these may be the same or different and may be present on the same or different atom in -Alk or in R⁷ as appropriate Thus for example, R⁷ may represent a -CH(R¹³)₂ group, such as a -CH(OH)Ar group where Ar is an aryl or heteroaryl group as defined below Clearly, when m is zero and no substituent R¹³ is present the alkylene, alkenylene or alkynylene chain represented by Alk becomes an alkyl, alkenyl or alkynyl group.

When R^{1 3} is a halogen atom it may be for example a fluorine, chlorine, bromine, or iodine atom

Esterified carboxyl groups represented by the group R¹³ include groups of formula -C0₂Alk¹ wherein Alk¹ is a straight or branched, optionally substituted C I -Θ alkyl group such as a methyl, ethyl, n-propyl, i-propyl, n-butyl, i-butyl, s-butyl or t-butyl group; a C6-ι₂arylCι-8alkyl group such as an optionally substituted benzyl, phenylethyl, phenylpropyl, 1 -naphthyl- methyl or 2-naphthylmethyl group; a C6-ι₂aryl group such as an optionally substituted phenyl, 1 -naphthyl or 2-naphthyl group; a C6-ι₂aryloxyC-| .₈alkyl group such as an optionally substituted phenyloxymethyl, phenyloxyethyl,

1 -naphthyloxymethyl, or 2-naphthyloxymethyl group, an optionally substituted C-i-ealkanoyloxyC-i-βalkyl group, such as a pivaloyloxymethyl, propionyloxyethyl or propionyloxypropyl group, or a Ce-^aroyloxyC-_i-salkyl group such as an optionally substituted benzoyloxyethyl or benzoyl- oxypropyl group Optional substituents present on the Alk¹ group include

R¹³ substituents described above.

When Alk is present in or as a substituent R ² it may be for example a methylene, ethylene, n-propylene, i-propylene, n-butylene, i-butylene, s-butylene, t-butylene, ethenylene, 2-propenylene, 2-butenylene_, 3-butenylene, ethynylene, 2-propynylene, 2-butynylene or 3-butynylene chain, optionally interrupted by one, two, or three -O- or -S-, atoms or -S(O)-, -S(0)₂- or -N(R ¹)- groups Optionally substituted cycloalkyl groups represented by the group R^{1 3} include optionally substituted C_5-7 cycloalkyl groups such as optionally substituted cyclopentyl or cyclohexyl groups.

Heterocycloalkyl groups represented by the group R¹² or R ⁴ include optionally substituted heteroC_3-6cycloalkyl groups containing one or two oxygen, sulphur or nitrogen atoms. Particular examples of such groups include optionally substituted azetid yl pyrrolidinyl, pipeπdinyl, piperazmyl, homopiperazinyl, morpholinyl or thiomorphol yl groups. The heterocyclo- alkyl group may be attached to the remainder of the molecule through any of its ring carbon atoms, or where present, ring nitrogen atom. Where the group R¹² is an -Alk-heterocycloalkyl group, Alk may be as defined above and the heterocycloalkyl portion may be as just defined, attached to Alk through any of its ring carbon atoms, or where present, ring nitrogen atom.

Optional subsituents which may be present on R¹², R¹³ or R ⁴ cycloalkyl or heterocycloalkyl groups include one or two C_{1 -6} alkyl, e.g. methyl or ethyl, hydroxyl (-OH) hydroxyC-|.₆alkyl, e.g. hydroxymethyl or hydroxyethyl, or C_{1 -6} alkoxy, e.g. methoxy or ethoxy groups. The substιtuent(s) may be present on any available ring carbon or nitrogen atom as appropriate.

Aryl and heteroaryl groups represented by the groups R^{1 3} or R ⁴ include for example optionally substituted monocyclic or bicyclic C_6--|₂ aromatic groups, or C-|_-9 heteroaromatic groups such as those described above in relation to the group R⁷.

Particularly useful atoms or groups represented by R¹² include fluorine, chlorine, bromine or iodine atoms, or Chalky!, C_{1 -6} alkylamino, d - ₆hydroxyalkyl, Cι_-6alkylthiol, d-₆alkoxy, hydroxyd-ealkoxy, aminod- ₆alkoxy, d-6alkylarr.inod-6a.koxy, Cι_-6dialkylaminoCι_-6alkoxy, optionally substituted C₅- cyclo-alkoxy, optionally substituted C _-7cycloalkyl, optionally substituted C₅-7cycloalkylamino, haloC_{1 -6}alkyl, halod-ealkoxy, Cι _-6alkylamino, amino (-NH₂), aminoCι .₆alkyl, C_{1 -6}dialkylamino, hydroxyCι.6 alkylamino, aminoC_{1 -6}alkylamino, d -θalkylaminod- ₆alkylamino, Cι .₆dialkylamιnoCι -₆alkylamino, C_{1 -6}alkylaminoC_{1 -6} dialkylamino, C-|.6dialkylaminoCι_-6dialkylamιno, nitro, cyano, hydroxyl (- OH), formyl [HC(O)-], carboxyl (-C0₂H), -CH₂C0₂H, -OCH₂C0₂H, -Cθ2Alk¹ [where Alk¹ is as defined above], -CH₂C0₂Alk¹ , - 6alkoxycarbonylCι-6alkoxy, d-6 alkanoyl, optionally substituted phenyl d - ₆alkanoyl, thiol (-SH), thiod-βalkyl, -SC(NH)NH₂, sulphoπyl (-SO3H), d - βalkylsulphonyl, optionally substituted phenylsulphonyl, ammosulphonyl (-SO2NH2), Cι-6alkylamιnosulphonyl, Cι-6dιalkylamιnosulphonyl, optionally substituted phenylamino-sulphonyl, carboxamido (-CONH2), Cι-6alkyl- ammocarbonyl, d -βdialkylammocarbonyl, optionally substituted phenylaminocarbonyl, ammocarbonylmethyl, C-| -6alkylamιnocarbonyl- methyl, optionally substituted benzylammocarbonylmethyl, -NHC(S)NH2 sulphonylammo (-NHSO2H) , d -6alkylsulphonylamιno, Ci -edialkyl- sulphonylamino, optionally substituted pheπylsulphonylamiπo, ammo- sulphonylammo (-NHSθ2NH₂), Cι-6alkylamιnosulphonylamιno, Ci-edialkyl- aminosulphonylammo, optionally substituted phenylaminosulphonylammo, aminocarbonylammo, Cι -6alkylamιnocarbonylamιno d-6dιalkylamιno- carbonylammo, phenylaminocarbonyiamino, d-6alkanoylamino, amιnoCι-6 alkanoylammo, optionally substituted pyridylcarboxyamino, d-6alkanoyl- amιnoCι-6alkyl, Ci -6 alkoxycarbonylamino, optionally substituted heteroC_3- 6cycloalkyl, pipendmyl, piperazmyl, 4-methyl-pιperazιnyl, homopipeprazinyl, or morpholinyl, optionally substituted heteroC3.6cycloalkylCι-6alkyl, pιperιdιnylCι-6alkyl, pιperazιnylCι-₆alkyl or morpholinylC-i βalkyl, optionally substituted heteroC3.6alkylCι-6alkylamιno, optionally substituted heteroC3- 6cycloalkylamιno, tetrazolyl, optionally substituted phenylamino, optionally substituted benzylamino, optionally substituted benzyloxy, or optionally substituted pyndiylmethylamino group

Where desired, two R¹² substituents may be linked together to form a cyclic group such as a cyclic ether, e.g. a d-βalkylenedioxy group such as a methylenedioxy or ethylenedioxy group

Especially useful R¹² substituents include for example fluorine, chlorine, bromine or iodine atoms, or a methylamino, ethylamino, hydroxymethyl, hydroxyethyl, methylthiol, ethylthiol, methoxy, ethoxy, π-propoxy, 2- hydroxyethoxy, 3-hydroxypropoxy, 4-hydroxybutoxy, 2-amιnoethoxy, 3- ammopropoxy, 2-(methylamιno)ethoxy, 2-(dιmethylamιno)ethoxy, 3- (dιmethylamιno)propoxy, cyclopentyloxy, cyclohexyl, cyclohexylammo, 2- hydroxycyclohexylammo, trifluoromethyl, tnfiuoromethoxy, methylamino, ethylamino, ammo (-NH)₂, aminomethyl, aminoethyl, dimethylamino, diethylamino, ethyl(methyl)amιno, propyl(methyl)amιno, 2-hydroxyethyl- amino, 3-hydroxypropylamιno, 4-hydroxybutylamιno, 2-amιnoethylamιno, 3- aminopropylammo, 4-amιnobutylamιno, 2-(methylamιno)ethylamιno, 2- (ethylamιno)ethylamιno, 2-(ι-propylamιno)ethylamιno, 3-(ι-propylamιno)- propylam o, 2-(dιmethylamιno)ethylamιno, 3-(dιmethylamιno)propylamιno, 2-(dιethylamιno)ethylamιno, 3-(dιethylamιno)propylamιno, 2-(methylamιno)- ethyl(methyl)amιno, 3-(methylamιno)propyl(methyl)amιno, 2-(dιmethyl- amιno)ethyl(methyl)amιno, 2-(dιmethylamιno)ethyl(ethyl)amιno, nitro, cyano, hydroxyl (-OH), formyl [HC(O)-], carboxyl (-C0₂H), -CH₂C0₂H - OCH₂C0₂H, -C0₂CH₃, -CO2CH2CH3, -CH₂C0₂CH₃, -CH₂C0₂CH₂CH₃, -CH C0 CH phenyl, t-butoxycarbonylmethoxy, acetyl, phenacetyl, thio (-SH), thiomethyl, thioethyl, -SC(NH)N H₂ , sulphonyl (-S0₂ H), methylsulphonyl, methylammosulphonyl, ethylammosulphonyl, dimethyl- ammosulphonyl, diethylammosulphonyl, carboxamido (-CONH₂), methyl- ammocarbonyl, ethylammocarbonyl, dimethylammocarbonyl, diethylammo- carbonyl, methylammocarboπylmethyl, -NHC(S)NH₂, sulphonylamino (-NHSO2H), methylsulphonylamino ethylsulphonylammo, dimethyl- sulphonylamino, diethylsulphonylammo, sulphonylamino (-HHSO2NH2), methylaminosulphonylammo, ethylaminosulphonylammo, dimethylammo- sulphonylam o, diethylaminosulphonylammo, methylammocarbonylamino_, ethylaminocarbonylamino, dimethylammocarbonylamino diethylammo- carbonylammo, acetylamino, aminomethylcarbonylamino, acetylammo- methyl, methoxycarbonylamino, ethoxycarbonylammo, t-butoxycarbonyl- ammo, pyrrolidinyl, pipendmyl, piperazmyl, 4-methylpιperazιnyl, homopipeprazinyl, morpholinyl, pyrrolιdιnylCι-₆alkyl, pιperιdιnyld-6alkyl, pιperazιnylC_{1 -6}alkyl, morpholιnylC_{1 -6}alkyl, 2-pyrrolιdιnylethylamιno, 2-{1 - methylpyrrolιdιnyl)ethylamιno, 1 -ethylpyrrolidinylmethylammo, pipendinyl- am o, 1 -benzylpιperιdιnyiamιno, 4-(methoxy)phenylamιno, 4-(3-hydroxy- propyl)phenylamιno, benzylammo, benzyloxy, pyridiylmethylamino group

It will be appreciated that where two or more R¹² substituents are present, these need not necessarily be the same atoms and/or groups. The presence of certain substituents in the compounds of formula (1 ) may enable salts of the compounds to be formed. Suitable salts include pharmaceutically acceptable salts, for example acid addition salts derived from inorganic or organic acids, and salts derived from inorganic and organic bases.

Acid addition salts include hydrochlorides, hydrobromides, hydroiodides, alkylsulphonates, e.g. methanesulphonates, ethanesulphoπates, or isethionates, arylsulphonates, e.g. p-toluenesulphonates, besylates or napsylates, phosphates, sulphates, hydrogen sulphates, acetates, tnfluoroacetates, propionates, citrates, maleates, fumarates, malonates, succmates, lactates, oxalates, tartrates and benzoates.

Salts derived from inorganic or organic bases include alkali metal salts such as sodium or potassium salts, alkaline earth metal salts such as magnesium or calcium salts, and organic am e salts such as morpholine, piperidine, dimethylamine or diethylamine salts.

Particularly useful salts of compounds according to the invention include pharmaceutically acceptable salts, especially acid addition pharmaceutically acceptable salts.

It will be appreciated that depending on the nature of the substituents R¹ - R³ and R⁵ - R⁷ the compounds of formula (1) may exist as geometrical isomers and/or may have one or more chiral centres so that enantiomers or diasteromers may exist. It is to be understood that the invention extends to all such isomers of the compounds of formula (1 ), and to mixtures thereof, including racemates.

In one class of compounds of formula (1 ) the groups R , R², R³, R⁴, R5_ R⁷ and X are as defined for formula (1 ), as is the group R⁶ except it is not an am o, substituted am o, nitro, carboxyl or esterified carboxyl group.

One particular class of compounds according to the invention has the formula (1 ) wherein R² and R³ is each an optionally substituted methyl or ethyl group. Particular examples of groups of these types include methyl, ethyl, halomethyl, e.g. -CH₂F, -CH₂CI, -CHF₂, -CHCI₂, -CF₃, -CCI₃, or haloethyl groups. In general however, R² and R³ is each preferably a methyl group. In compounds of this class and in general in compounds of formula (1 ) R¹ may in particular be an optionally substituted methoxy group. Particular examples of such R¹ groups include -OCH₂F, -OCH₂CI, -OCHF2, -OCHCI2, -OCF_3l -OCCI3 or, especially, methoxy groups.

In a further preferred class of compounds of formula (1 ) R⁴ is preferably a hydrogen atom.

The groups R⁵ and R⁶ in compounds of formula (1 ) are each preferably a hydrogen atom.

In yet another preference, X in compounds of formula (1 ) is a direct bond, an oxygen or sulphur atom or a -N(R^{1 1} )- group. Especially useful compounds of this type are those whereinX is a direct bond, a sulphur atom or a -N(R^{1 1} )-, particularly a -NH-, group.

R⁷ in compounds of formula (1) is preferably an optionally substituted aromatic or heteroaromatic group.

A further class of compounds according to the invention has the formula 1 (a):

wherein R⁵ and R⁶ are as defined for formula (1 ), X is a direct bond, an oxygen or sulphur atom, or a group -N(R^{1 1} )- and R⁷ is an optionally substituted aromatic or heteroaromatic group, and the salts, solvates, hydrates and N-oxides thereof.

In these compounds, R⁵ is preferably a hydrogen atom. R⁶ is preferably a group -X R^6a where X¹ is as defined for formula (1 ) and R^{6 a} is an optionally substituted straight or branched chain alkyl group, or R⁶ is especially a hydrogen atom.

X in compounds of formula (1 a) is preferably a direct bond, a sulphur atom, or a -N(R^{1 1})- group, particularly a -NH-group.

The aromatic or heteroaromatic R⁷ group in compounds of formulae (1 ) or 1 (a) in general may be as defined previously for compounds of formula (1 ). In one preference, however, R⁷ is an optionally substituted phenyl, 1 - or 2-naphthyl or heteroaromatic group containing one or two oxygen, sulphur and/or nitrogen atoms. Thus in particular R⁷ may be an optionally substituted phenyl, 1 - or 2-naphthyl, pyrrolyl, furyl, thienyl, indolyl, pyrazolyl, thiazolyl, [2,3-dιhydro]benzofuryl, benzothiazolyl, 2-pyrιdyl, 3- pyridyl or 4-pyπdyl group. Particularly useful groups include optionally substituted phenyl, 2-pyπdyl, 3-pyrιdyl or 4-pyπdyl groups. The aromatic or heteroaromatic group may in particular be attached to the remainder of the compound of formula (1 ) through any available ring carbon atom.

In general, the optional substituents which may be present on aromatic or heteroaromatic R⁷ groups in compounds of formulae (1 ) or (1 a) include one, two, or three R¹² substituents as generally and particularly described above and hereinafter in the Examples. Particularly useful R^{1 2} substituents include -NHR¹⁴, -AlkNH₂, -AlkNHR¹⁴, -OR¹⁴, -AlkC0₂H or -AlkC0₂Alk¹ groups where R ⁴, Alk and Alk¹ are as generally and particularly defined above. Useful members of these substituents include those wherein R¹⁴ is an -Alk, -AlkNH2 or -Alk-heterocycloalkyl group. In t hese, and the other preferred substituents just mentioned, Alk and Alk¹ when present is each preferably a d -6alkyl group.

Particularly useful compounds according to the invention include: N,N'-Bis(3,4,5-Trιmethoxyphenyl)-2,4-pyrιmιdιnedιamιne; 4-(2-(2-Dιmethylamιnoethylamιno)pyrιdιn-5-yl)-N-(3,4,5-trιmethoxyphenyl)- 2-pyπmιdιneamιne,

4-(2-(4-Hydroxybutylamιno)pyπdιn-5-yl)-N-(3,4,5-tπmethoxyphenyl)-2- pyπmidineamine; 4-(3-(3-Ammopropoxy)phenyl)-N-(3,4,5-tπmethoxyphenyl)-2-pyrιmιdιne- amine;

N4-(3,4-Dιmethoxyphenyl)-N2-(3,4,5-trιmethoxyphenyl)-2,4-pyπmιdιnedι- amme;

4-(4-(2-Hydroxyethoxy)phenyl)-N-(3,4,5-tnmethoxyphenyl)-2-pyrιmιdιne- amme;

4-(2-(3-(Morpholιno)propylamιno)pyrιdιn-5-yl)-N-(3,4,5-tπmethoxyphenyl)-

2-pyrιmιdιneamιne;

4-(2-(2-(1-Methylpyrrolιdιn-2-yl)ethylamιno)pyπdιn-5-yl)-N-(3,4,5- trιmethoxyphenyl)-2-pyrιmιdιneamιne, N4-(4-(Ethoxycarbonylmethyl)phenyl)-N2-(3,4,5-trιmethoxyphenyl)-2,4- pyπmidinediamine;

N4-(4-Hydroxyphenyl)-N2-(3,4,5-tπmethoxyphenyl)-2,4-pyπmιdιnedιamιne and

N4-(4-(3-Amιnopropoxy)phenyl)-N2-(3,4,5-trιmethoxyphenyl)-2,4- pyπmidinediamine; and the salts, solvates, hydrates and N-oxides thereof

Compounds according to the invention are potent and selective inhibitors of protein kinases as demonstrated by differential inhibition of enzymes such as EGFr kinase, p56^lck kinase, ZAP-70 kinase, Csk kinase and p59^fyⁿ kinase The ability of the compounds to act in this way may be simply determined by employing tests such as those described in the Examples hereinafter.

The compounds according to the invention are thus of particular use in the prophylaxis and treatment of diseases in which inappropriate protein tyrosine kinase action plays a role, for example in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis, and systemic lupus erythematosus, in transplant rejection, in graft v host disease, in hyperproliferative disorders such as tumours, psoriasis, in pannus formation in rheumatoid arthritis, restenosis following angioplasty and atherosclerosis, and in diseases in which cells receive pro-inflammatory signals such as asthma, inflammatory bowel disease and pancreatitis

For the prophylaxis or treatment of disease the compounds according to the invention may be administered as pharmaceutical compositions, and according to a further aspect of the invention we provide a pharmaceutical composition which comprises a compound of formula (1) together with one or more pharmaceutically acceptable carriers, excipients or diluents

Pharmaceutical compositions according to the invention may take a form suitable for oral, buccal, parenteral, nasal, topical or rectal administration, or a form suitable for administration by inhalation or insufflation

For oral administration, the pharmaceutical compositions may take the form of, for example, tablets, lozenges or capsules prepared by conventional means with pharmaceutically acceptable excipients such as binding agents (e g pregelatinised maize starch, polyvinylpyrrolidone or hydroxypropyl methylcellulose); fillers (e g lactose, microcrystalline cellulose or calcium hydrogen phosphate), lubricants (e g magnesium stearate, talc or silica), disintegrants (e g potato starch or sodium glycollate), or wetting agents (e g sodium lauryl sulphate) The tablets may be coated by methods well known in the art Liquid preparations for oral administration may take the form of, for example, solutions syrups or suspensions, or they may be presented as a dry product for constitution with water or other suitable vehicle before use Such liquid preparations may be prepared by conventional means with pharmaceutically acceptable additives such as suspending agents, emulsifying agents, non-aqueous vehicles and preservatives The preparations may also contain buffer salts, flavouring, colouring and sweetening agents as appropriate

Preparations for oral administration may be suitably formulated to give controlled release of the active compound

For buccal administration the compositions may take the form of tablets or lozenges formulated in conventional manner The compounds for formula (1 ) may be formulated for parenteral administration by injection e.g. by bolus injection or infusion. Formulations for injection may be presented in unit dosage form, e.g. in glass ampoule or multi dose containers, e.g. glass vials. The compositions for injection may take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilising, preserving and/or dispersing agents. Alternatively, the active ingredient may be in powder form for constitution with a suitable vehicle, e.g. sterile pyrogen-free water, before use.

In addition to the formulations described above, the compounds of formula (1 ) may also be formulated as a depot preparation. Such long acting formulations may be administered by implantation or by intramuscular injection.

For nasal administration or administration by inhalation, the compounds for use according to the present invention are conveniently delivered in the form of an aerosol spray presentation for pressurised packs or a nebuliser, with the use of suitable propellant, e.g. dichlorodifluoromethane, trichloro- fluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas or mixture of gases.

The compositions may, if desired, be presented in a pack or dispenser device which may contain one or more unit dosage forms containing the active ingredient. The pack or dispensing device may be accompanied by instructions for administration.

The quantity of a compound of the invention required for the prophylaxis or treatment of a particular condition will vary depending on the compound chosen, and the condition of the patient to be treated. In general, however, daily dosages may range from around 100ng/kg to 100mg/kg e.g. around 0.01 mg/kg to 40mg/kg body weight for oral or buccal administration, from around 10ng/kg to 50mg/kg body weight for parenteral administration and around 0.05mg to around 1000mg e.g. around 0.5mg to around 1000mg for nasal administration or administration by inhalation or insufflation. The compounds of the mvention may be prepared by a number of processes as generally described below and more specifically in the Examples hereinafter. In the following process description, the symbols R¹-R⁷ and X when used in the formulae depicted are to be understood to represent those groups described above in relation to formula (1 ) unless otherwise indicated. In the reactions described below, it may be necessary to protect reactive functional groups, for example hydroxy, amino, thio or carboxy groups, where these are desired in the final product, to avoid their unwanted participation in the reactions. Conventional protecting groups may be used in accordance with standard practice [see, for example, Green, T. W. in "Protective Groups in Organic Synthesis", John Wiley and Sons, 1981 ]. In some instances, deprotection may be the final step in the synthesis of a compound of formula (1 ) and the processes according to the invention described hereinafter are to be understood to extend to such removal of protecting groups.

Thus according to a further aspect of the invention, a compound of formula (1) wherein X is a direct bond may be prepared by reaction of a guanidine of formula (2):

or a salt thereof with an enammone of formula (3):

R⁷C0C(R6)C(R5)N(R¹5)(R16) ₍₃₎

where R^{1 5} and R^{1 6}, which may be the same or different is each a C_{1 -6} alkyl group.

The reaction may be performed in a solvent, for example a protic solvent such as an alcohol, e.g. ethanol, methoxyethanol or propanol, optionally in the presence of a base e.g. an alkali metal base, such as sodium hydroxide or potassium carbonate, at an elevated temperature, e.g. the reflux temperature.

Salts of the compounds of formula (2) include acid salts such as inorganic acid salts e.g. hydrochlorides or nitrates.

Intermediate guanidines of formula (2) may be prepared by reaction of the corresponding aniline of formula (4):

with cyanamide at an elevated temperature.

The reaction may be performed in a solvent such as ethanol at an elevated temperature, e.g. up to the reflux temperature. Where it is desired to obtain a salt of a guanidine of formula (2), the reaction may be performed in the presence of a concentrated acid, e.g. hydrochloric or nitric acid.

The anilines of formula (4) are either known compounds or may be obtained by conventional procedures, for example by hydrogenation of the corresponding nitro derivatives using for example hydrogen in the presence of a metal catalyst in a suitable solvent, for example as more particularly described in the interconversion reactions discussed below or by use of the corresponding nitro derivative and a reducing agent such as a sodium hydrosulphite in a solvent such as ethanol at an elevated temperature such as the reflux temperature. The nitrobenzenes for this particular reaction are either known compounds or may be prepared using similar methods to those used for the preparation of the known compounds, for example by treatment of the corresponding benzene with nitric acid in the prsence of an acid such as acetic acid at around ambient to the reflux temperature

Intermediate enam ones of formula (3) may be prepared by reaction of an acetyl derivative R⁷COCH₂R⁶ with an acetal (R¹⁶)(R ⁵)NCR⁵(OCH₃)₂ at an elevated temperature The starting materials for this reaction are either known compounds of may be prepared by methods analogous to those used for the preparation of the known compounds.

In another process according to the invention, a compound of formula (1 ) where X is a linker atom or group may be prepared by reaction of an intermediate of formula (5)

where L is a leaving atom or group, with a reagent R X²H where X² is a linking atom or group as defined above.

Particular leaving atoms or groups represented by L include for example halogen atoms, e.g. bromine, iodine or chlorine atoms, and sulphonyloxy groups, e.g. alkylsulphonyloxy groups, such as tπfluoromethylsulphonyl- oxy, and aryisulphonyloxy groups, such as p-toluenesulphonyloxy

The reaction may be performed in the presence of a base, for example an organic base such as an organic amme, e.g triethylamine or an inorganic base, for example a hydride such as sodium hydride, an alkoxide such as potassium t-butoxide, or a carbonate such as caesium or potassium carbonate, where necessary in the presence of a dipolar aprotie solvent such as an ether, e.g. a cyclic ether such as tetrahydrofuran, or an amide, e.g. a substituted amide such as dimethylformamide, at a suitable temperature e g from around room temperature to around 90°C

Intermediates of formula (5) may be prepared by reaction of the corresponding pyπmidinones (where -N=C(L)- in formula (5) is -NH-C(O)-) with a halide using standard procedures, for example by reaction with a reagent PO(Hal)3 (where Hal is a halogen atom), e g POCI₃, or RS0 Hal (where R is an optionally substituted alkyl or aryl group) where necessary at an elevated temperature

The starting pyπmidinones may be prepared by reaction of a guanidine of formula (2) or a salt thereof with a β-ketoester [for example a compound CH₃CH₂C (0)OCH(R⁶)C(0)R⁵ (where R5 IS an optionally substituted alkyl, alkenyl or alkynyl group)] or a functional analogue thereof where it is desired to obtain compounds wherein R⁵ is a hydrogen atom, in the presence of a base, for example an alkoxide such as sodium methoxide in a solvent, for example a protic solvent, such as an alcohol, e g methanol at an elevated temperature, e g up to around the reflux temperature

In a further process according to the invention, a compound of formula (1 ) may be prepared by displacement of a leaving atom or group in a pyrimidine of formula (6)

[where L is a leaving atom or group as defined above] with an aniline of formula (4)

The reaction may be performed at an elevated temperature, for example the reflux temperature, where necessary in the presence of a solvent, for example a ketone such as acetone, an alcohol such as ethanol or 2- ethoxyethanol or an aromatic hydrocarbon such as toluene, optionally in the presence of a base, for example an organic amme such as triethylamide or pyridine, or an acid, for example an inorganic acid such as hydrochloric acid.

Intermediate pyrimidines of formula (6) may be prepared by displacement of a leaving group from a pyrimidine of formula (7):

[where L¹ is a leaving atom or group as described above for the group L] using a nucleophilic reagent R⁷XH. The reaction may be performed as just described in relation to the preparation of compounds of formula (1 ) from the intermediate pyrimidines of formula (6).

The pyrimidines of formula (7) and the nucleophilic reagents R⁷XH are either known compounds or may be prepared using methods analogous to those used for the preparation of the known compounds.

In another process according to the invention, a compound of formula (1 ) wherein X is a -C(O)- group may be prepared by treating a carboxamide of formula (8):

[where X³ is a carboxamide group] with a reagent R⁷MHal¹ (where M is a metal atom such as an zinc atom and Hal¹ is a halogen atom such as a bromine atom).

The group X³ in intermediates of formula (8) may be for example a group -CONR¹5R₁₆ or -CON(R¹⁵)(OR¹6).

The reaction may be performed in a solvent such as an ether, e.g. a cyclic ether such as a tetrahydrofuran, at a low temperature, e g around -78°C.

Intermediate carboxamides of formula (8) may be prepared by reaction of the corresponding acid of formula (9):

or a reactive derivative thereof with a reagent R¹⁵R¹⁶NH or R¹⁵R¹60NH in the presence of a base e.g. an organic amme such as tnet hylamine in a solvent such as dichloromethane at a low temperature, e.g. around -20° to 0°C.

Intermediate acids of formula (9) may be prepared by heating the corresponding nitrile in the presnce of a base such as sodium hydroxide in a solvent such as ethanol. The nitrile starting material may be prepared by heating 2-chloro-4-cyano-pyhmidine with the appropriate aniline in the presence of a base such as triethylamine in a solvent such as ethanol at the reflux temperature.

Acids of formula (9) may also be used to generate compounds of formula (1 ) wherein X is a -NHC(0)0- group by reaction with an azide, for example diphenylphosphorylazide, and an alcohol R OH in the presence of a base such as triethylamine at an elevated temperature, e.g. the reflux temperature.

Compounds of formula (1 ) may also be prepared by interconversion of other compounds of formula (1 ) and it is to be understood that the invention extends to such interconversion processes. Thus, for example, standard substitution approaches employing for example alkyiation, arylation, aeylation, thioacylation, sulphonylation, formylation or coupling reactions may be used to add new substitutents to and/or extend existing substituents in compounds of formula (1 ). Altematively existing substituents in compounds of formula (1 ) may be modified by for example oxidation, reduction or cleavage reactions to yield other compounds of formula (1 ).

The following describes in general terms a number of approaches which can be employed to modify existing R¹ , R², R³, R⁴, R5 and/or R⁶ groups in compounds of formula (1 ). It will be appreciated that each of these reactions may only be possible where an appropriate functional group exists in a compound of formula (1 ). Equally, any of the following reactions may be used to generate appropriately substituted intermediates of formulae (2), (4), (5), (6) and (7) for use in the preparat on of compounds of formula (1 ). Thus, for example alkyiation or arylation of a compound of formula (1 ) may be achieved by reaction of the compound with a reagent R⁴L, AlkL or ArL, where R⁴, Alk, Ar and L are as previously defined.

The alkyiation or arylation reaction may be carried out in the presence of a base, e.g. an inorganic base such as a carbonate, e g. caesium or potassium carbonate, an alkoxide, e g. potassium t-butoxide, or a hydride, e.g. sodium hydride, in a dipolar aprotie solvent such as an amide, e.g. a substituted amide such as dimethylformamide or an ether, e g. a cyclic ether such as tetrahydrofuran, at around 0°C to around 40°C

In a variation of this process the leaving group L may be alternatively part of the compound of formula (1 ) and the reaction performed with an appropriate nucleophilic reagent in a solvent such as an alcohol, e g ethanol, at an elevated temperature, e.g the reflux temperature.

In another general example of an interconversion process, a compound of formula (1 ) may be acylated or thioacylated The reaction may be performed for example with an acyl halide or anhydride in the presence of a base, such as a tertiary amme e g triethylamine in a solvent such as a halogenated hydrocarbon, e.g dichloromethane or carbon tetrachloride, or an alcohol, e.g methanol at for example ambient temperature, or by reaction with a thioester in an inert solvent such as tetrahydrofuran at a low temperature such as around 0°C The reaction is particularly suitable for use with compounds of formula (1) containing primary or secondary ammo groups.

In a further general example of an interconversion process, a compound of formula (1 ) may be formylated, for example by reaction of the compound with a mixed anhydride HCOOCOCH3 or with a mixture of formic acid and acetic anhydride

Compounds of formula (1 ) may be prepared in another general interconversion reaction by sulphonylation, for example by reaction of the compound with a reagent AlkS(O)₂L, or ArS(O)2L in the presence of a base, for example an inorganic base such as sodium hydride in a solvent such as an amide, e.g a substituted amide such as dimethylformamide at for example ambient temperature The reaction may in particular be performed with compounds of formula (1 ) possessing a primary or secondary ammo group

In further examples of interconversion reactions according to the invention compounds of formula (1 ) may be prepared from other compounds of formula (1 ) by modification of existing functional groups in the latter

Thus in one example, ester groups -C0₂Alk¹ in compounds of formula (1 ) may be converted to the corresponding acid [-C0₂H] by acid- or base- catalysed hydrolysis or by catalytic hydrogenation depending on the nature of the group Alk¹ Acid- or base-catalysed hydrolysis may be achieved for example by treatment with an organic or inorganic acid_, e g trifluoroacetic acid in an aqueous solvent or a mineral acid such as hydrochloric acid in a solvent such as dioxan or an alkali metal hydroxide, e.g lithium hydroxide in an aqueous alcohol, e g aqueous methanol Catalytic hydrogenation may be carried out using for example hydrogen in the presence of a metal catalyst, for example palladium on a support such as carbon in a solvent such as an ether, e g tetrahydrofuran or an alcohol_, e g methanol Similarly, base-catalysed hydrolysis with for example an alkali metal hydroxide such as sodium hydroxide in a solvent such as an alcohol e g ethanol may be used to convert a >NS0₂Alk or >NS0₂Ar group to a >N-H group

In a second example, -OAlk² [where Alk² represents an alkyl group such as a methyl group] groups in compounds of formula (1 ) may be cleaved to the corresponding alcohol [-OH] by reaction with boron tribromide in a solvent such as a halogenated hydrocarbon, e g dichloromethane at a low temperature, e g around -78°C

Alcohol [-OH] groups may also be obtained by hydrogenation of the corresponding -OCH₂Ar group using for example hydrogen in the presence of a metal catalyst, for example palladium on a support such as carbon in a solvent such as ethanol in the presence of ammonium formate In another example, -OH groups may be generated from the corresponding ester [-Cθ2Alk] by reduction using for example a complex metal hydride such as lithium aluminium hydride.

In a further example, alcohol -OH groups in compounds of formula (1 ) may be converted to a corresponding -OAlk or -OAr group by coupling with a reagent AlkOH or ArOH in a solvent such as tetrahydrofuran in the presence of a phosphine, e.g triphenylphosphine and an activator such as diethyl-, diisopropyl-, or dimethylazodicarboxylate.

In another example of an interconversion reaction, amines of formula (1) may be alkylated using a reductive alkyiation process employing an aldehyde and a borohydride, for example sodium tπacetoxyborohydπde, in a solvent such as dichloromethane, in the presence of an acid such as acetic acid at around ambient temperature

Aminosulphonylammo [-NHSO2NH2] groups in compounds of formula (1 ) may be obtained, in another example, by reaction of a corresponding amme [-NH2] with sulphamide in the presence of an organic base such as pyridine at an elevated temperature, e g the reflux temperature

In a further example, amme [-NH2] groups in compounds of formula (1 ) may be obtained by hydrolysis from a corresponding imide by reaction with hydrazine in a solvent such as an alcohol, e g ethanol at ambient temperature.

In another example, a nitro [-NO2] group may be reduced to an amme [- NH₂], for example by catalytic hydrogenation as just described, or by chemical reduction using for example a metal, e.g. tin or iron, in the presence of an acid such as hydrochloric acid, optionally in a solvent such as an alcohol, e.g. methanol

In a further example of an interconversion process, a tetrazole substituent may be obtained from the corresponding nitrile by treatment of the latter with an azide, e.g. sodium azide, in a solvent such as a substituted amine, e.g. dimethylformamide at an elevated temperature. N-oxides of compounds of formula (1 ) may be prepared for example by oxidation of the corresponding nitrogen base using an oxidising agent such as hydrogen peroxide in the presence of an acid such as acetic acid, at an elevated temperature, for example around 70°C to 80°C, or altematively by reaction with a peracid such as peracetic acid or 3- chloroperoxybenzoic acid in a solvent, e.g. dichloromethane, at ambient temperature.

Where salts of compounds of formula (1 ) are desired, these may be prepared by conventional means, for example by reaction of a compound of formula (1 ) with an appropriate acid or base in a suitable solvent or mixture of solvents, e.g. an organic solvent such as an ether, e.g. diethylether, or an alcohol, e.g. ethanol.

The following Examples illustrated the invention. In the Examples all ¹ Hnmr were run at 300MHz unless specified otherwise. All temperatures are in °C. The following abbrevioations are used: DMSO - dimethylsulphoxide; DMF - dimethylformamide; THF - tetrahydrofuran.

EXAMPLE 1

4-Phenyl-N-(3.4.5-trimethoxyphenyl)-2-pyrimidineamine

To a solution of 3,4,5-(tπmethoxyphenyl)guanιdιne nitrate ( l .89g, 6.57mmol) and 3-dιmethylamιno-1 -phenyl-2-propen-1 -one ( 1 .1 5g , 6.57mmol) in propan-2-ol (20ml) was added powdered sodium hydroxide (289mg, 7.23mmol) and the mixture refluxed for 18h. On cooiing (0°) the resulting precipitate was collected and washed with propan-2-ol and water, and then subjected to column chromatography [silica 1 % methanol- CH2CH2] to afford the title compound (730mg) after recrystallisation from ethyl acetate as a yellow solid m.p. 146°. δπ (CDCI3) 3.85 (3H, s), 3.91 (6H, s), 7.07 (2H, s), 7.17 (1 H, d, J 5.2Hz), 7.18 (1 H, s, NH), 7.46-7.50 (3H, m), 8.10 (2H, m), and 8.46 (1 H, d, J 5.2Hz).

The guanidine starting material was prepared by heating a mixture of

3,4,5-trimethoxyaniline (5.49g, 30. Ommol), cyanamide [Aldrich, 50% solution in water w/v] (3.50ml, 45. Ommol) and concentrated nitric acid

(2.10ml, 300mmol) in ethanol (30ml). The solid which formed on cooling to room temperature was collected by filtration, washed with ethanol and dried in vacuo to give the desired product (4.60g) as a grey solid m.p. 187°. δ_H (d^DMSO) 3.65 (3H, s), 3.77 (6H, s), 6.54 (2H, s), 7.27 (4H, br s), and 9.46 (1 H, s).

The following compounds of Examples 2-35 were prepared in a similar manner using the above guanidine starting material and the appropriate enammone:

EXAMPLE 2

4-ι3-Pyridvπ-N-(3.4.5-trimethoxyphenyπ-2-pyrimidineamine from 3,4,5-(tπmethoxyphenyl)guanιdιne nitrate (1.33g, 4.60mmol), 3- dιmethylamιno-1 -(3-pyrιdy!)-2-propen-1 -one (812mg, 4.60mmol) and sodium hydroxide (203mg, 5.07mmol) to give the title compound (290mg) as a green solid m.p. 155°. 5H (CDCI₃) 3.63 (3H, s), 3.78 (6H, s), 7.28 (2H, s), 7.47 (1 H, d, J 5.1 Hz), 7.56 (1 H, m), 8.49 (1 H, m), 8.58 (1 H, d, J 5.1 Hz), 8.71 (1 H, dd, J 4.8, 1.5Hz), 9.35 (1 H, d, J 2.0Hz) and 9.61 (1 H, s).

EXAMPLE 3 4-(4-Pyridvπ-N-⁽3.4.5-trimethoxyphenyπ-2-pyrimidineamine from 3,4,5-(trιmethoxyphenyl)guanιdιne nitrate (1.44g, 5. Ommol), 3- dιmethylamιno-1 -(4-pyrιdyl)-2-propene-1 -one (880mg, 5. Ommol) and sodium hydroxide (220mg, 5.5mmoi) to give the title compound (765mg) as a green solid m.p. 205°. 6H (d⁶DMSO) 3.63 (3H, s), 3.79 (6H, s), 7.27 (2H, s), 7.49 (1 H, d, J 5.1 Hz), 8.08 (2H, dd, J 4.5, 1.6Hz), 8.64 (1 H, d, J 5.1 Hz), 8.76 (2H, dd, J 4.5, 1.6Hz), and 9.66 (1 H, br s).

EXAMPLE 4 4-(2-Furyl)-N-3.4.5-ttrimethoχy^phenyn-2-pyrimidineamine from 3,4,5-(trimethoxyphenyl)guanidine nitrate (1 .66g, 5.75mmol), 3- dιmethylamino-1 -(2-furyl)-2-propen-1 -one) (950mg, 5.75mmol) and sodium hydroxide (253mg, 6.33 mmol) to give the title compound (350mg) as a yellow solid m.p. 139°. δ_H (CDCI3) 3.83 (3H, s), 3.90 (6H, s), 6.56 (1 H, m), 7.01 (2H, s), 7.06 (1 H, d, J 5.1 Hz), 7.18 (1 H, ), 7.25 (1 H, s), 7.58 (1 H, d, J 2.3 Hz), and 8.42 (1 H, d, J 5.1 Hz). EXAMPLE 5

4-(3.4.5-Trlmethoxyphenyπ-N-(3.4.5-trimethoxyphenylV2-pyrimidine- amine from 3,4,5-(trιmethoxyphenyl)guanιdine nitrate (1 .08g, 3.77mmol), 3-dι- methylamιno-1 -(3,4,5-trιmethoxyphenyl)-2-propen-1 -one (1 .Og, 3.77mmol) and sodium hydroxide (166mg) to give the title compound (67mg) as a colourless solid m.p. 187°. δ_H (CDCI₃) 3.83 (3H, s), 3.88 (6H, s) , 3.92 (3H, s), 3.94 (6H, s), 6.99 (2H, s), 7.09 (1 H, d, J 5.2Hz), 7.23 (1 H, br s), 7.32 (2H, s) and 8.45 (1 H, d, J 5.2Hz).

EXAMPLE 6

4-f5-(2.3-Dihvdrobenzofuryl) -N-(3.4.5-trimethoχyphenvn-2- pyrimidineamine from 3,4,5-(tπmethoxyphenyl)guanιdιne nitrate ( 1 19g, 4 14mmol), 1 -(5- (2,3-dιhydrobenzofuryl)-3-dιmethylamιno-2-propen- 1 -one (900mg , 4.14mmol) and sodium hydroxide (182mg, 4.56mmol) to give the title compound (450mq) as a yellow solid m.p. 160°. δμ (CDCI3) 3.26 (2H, t, J 8.7Hz), 3.83 (3H, s), 3.90 (6H, s), 4.66 (2H, t, J 8.7Hz), 6.86 (1 H, d, J 8.3Hz), 7.06 (3H, m), 7.17 (1 H, s), 7.88 (1 H, d, 8.3Hz), 8.02 (1 H, s), and 8.38 ( 1 H, d, J 5.3Hz).

EXAMPLE 7

4-(1 -Phenylsulphonylindol-3-yl)-N-(3.4,5-trimethoxyphenyh-2- pyrimidineamine from 3,4,5-(trιmethoxyphenyl)guanιdιne nitrate (820mg, 2.84mmol), 3- dιmethylamιno-1 -(1 -phenylsulphonylιndol-3-yl)-2-propen-1 -one (1 .0g, 2.84mmol), and sodium hydroxide (125mg, 3.12mmol) to give the title compound (380mg) as a yellow solid m.p. 202°. δH (d⁶DMSO) 3.30 (3H, s), 3.63 (6H, s), 7.19 (2H, s), 7.33 (1 H, t, J 7.3Hz), 7.44 (2H, m), 7.61 (2H, m), 7.71 (1 H, m), 7.99 (1 H, d, J 8.3Hz), 8.09 (2H, d, J 7.4Hz), 8.49 (1 H, d, J 5.2Hz), 8.60 (1 H, d, J 7.9Hz), 8.74 (1 H, s) and 9.46 (1 H, s).

EXAMPLE 8 4-<2-Thiazolvπ-N-ι3.4.5-trimethoxyphenvn-2-pyrimidineamine from 3,4,5-(trιmethoxyphenyl)guanidιne nitrate (1 .27g, 4.40mmol) , 3- dιmethylamιno-1 -(2-thιazolyl)-2-propen-1 -one (802mg, 4 40mmol), and sodium hydroxide (194mg, 4.84mmol) to give the title compound (520mg) as a green solid m.p. 159°. δH (d⁶DMSO) 3.63 (3H, s), 3.82 (6H, s), 7.24 (2H, s), 7.45 (1 H, d, J 4.9Hz), 8.07 (1 H, d, J 3.0Hz), 8.62 (1 H, d, J_4.8Hz) and 9.70 (1 H, s).

EXAMPLE 9

4-ι3-Thienvπ-N- 3.4.5-Trimetho^χyphenyh-2-^pyrimidineamine from 3,4,5-(trimethoxyphenyl)guanidine nitrate (1.27g, 4.41 mmol), 3- dimethylamino-1 -(3-thienyl)-2-propen-1 -one (0.80g, 4.41 mmol) to give the title compound (365mα. as a vellow solid m.p. 163°. δπ (d⁶DMSO) 3.62 (3H, s), 3.78 (6H, s), 7.26-7.27 (3H, m), 7.68-7.71 (1 H, m), 7.78 (1 H, d, J 4.9Hz), 8.34-8.36 (1 H, m), 8.47 (1 H, d, J 5.0Hz) and 9.44 (1 H, s).

EXAMPLE 10 4-⁽2-Naphthvh-N-⁽3.4.5-trimetho^χy^phenyl⁾-2-pyrimidineamine from 3,4,5-(trimethoxypheny)guanidine nitrate (1.02g, 3.55mmol), 3- dimethylamino-1 -(2-naphthyl)-2-propen-1 -one (800mg, 3.55mmol) and sodium hydroxide (156mg, 3.90mmol) to give the title compound (310mg) as a yellow solid m.p. 156°. δπ (d⁶DMSO) 3.64 (3H, s), 3.82 (6H, s), 7.36 (2H, s), 7.53 (1 H, d, J 6.2Hz), 7.58-7.61 (2H, m), 7.97-8.07 (3H. m), 8.29 (1 H, d, J 8.5Hz), 8.58 (1 H, d, J 5.1 Hz), 8.76 (1 H, s), and 9.58 (1 H. br s).

EXAMPLE 11 4-⁽3-Nitrophenyn-N-(3.4.5-trimethoxyphenvn-2-^pyrimidineamine from 3,4,5-(trimethoxyphenyi)guanidine nitrate (3.93g, 13.63mmol), 3- dimethylamino-1-(3-nitrophenyl)-2-propen-1 -one (3.0g, 13.63mmol) and sodium hydroxide (545mg, 13.63mmol) to give the title compound (250mg) as a yellow solid, m.p. 184-185°. MS m/z 383 (M+H)+.

EXAMPLE 12

4-⁽4-Nitrophenyπ-N-⁽3.4.5-trimethoxy^phenvn-2-pyrimidineamine from 3,4,5-(trimethoxyphenyl)guanidine nitrate (1 .3g, 4.5mmol), 3- dimethylamino-1 -(4-nitrophenyl)-2-propen-1 -one (1.0g, 4.5mmol) and sodium hydroxide (200mg) to give the title compound (550mg) as an orange solid m.p. 196°. MS m/z 383 (M+H)+. EXAMPLE 13 4-(3-Bromophenyπ-N-(3.4.5-trimethoxyphenyl)-2-pyrimidineamine from 3,4,5-(trimethoxyphenyl)guanidine nitrate (1.63g, 5.66mmol), 1 -(3- bromophenyl)-3-dimethylamino-2-propen-1-one (1.5g, 5.66mmol) and sodium hydroxide (250mg, 6.23mmol) to give the title compound (785mg) as a yellow solid m.p. 145°. MS m/z 418 (M+H)⁺.

EXAMPLE 14 4-<^,Pyrrpl-2-vh-N-f3.4.5-trimethoxyphenyπ-2-pyrimidineamine from 3,4,5-(trimethoxyphenyl)guanidine nitrate (1.15g, 4. Ommol), 3- dimethylamino-1 -(pyrrol-2-yl)-2-propen-1 -one (656g, 4. Ommol) and sodium hydroxide (176mg, 4.4mmol) to give the title compound (10mg) as a yellow solid m.p. 150°. MS m/z 327 (M+H)⁺.

EXAMPLE 15

4-(3.4.-Methylenedioxyphenyl)-N-(3,4,5-trimethoxyphenyl)-2- pyrimidineamine from 3,4,5-(trimethoxyphenyl)guanidine nitrate (1.15g, 4. Ommol), 3- dimethylamino-1 -(3,4-methylenedioxyphenyl)-2-propen-1 -one (8.76g, 4. Ommol) and sodium hydroxide (176mg) to give the title compound (160mg) as a yellow solid m.p. 180°. MS m/z 382 (M+H)⁺.

EXAMPLE 16 4-(Pyrazin-2-yπ-N-f3.4.5-trimethoxyphenyl)-2-pyrimidineamine from 3,4,5-(trimethoxyphenyl)guanidine nitrate (1.15g, 4. Ommol), 3- dimethylamino-1 -(pyrazin-2-yl)-2-propen-1 -one (708mg, 4. Ommol) and sodium hydroxide (176mg) to give the title compound (254mg) as a yellow solid, m.p. 181-182°. MS m/z 340 (M+H)⁺.

EXAMPLE 17

4-« fert-Butyπ-N-t3.4.5-trimethoxyphenyπ-2-pyrimidineamine from 3,4,5-(trimethoxyphenyl)guanidine nitrate (1.2g, 4.2mmol), 1 -tert- butyl-3-dimethylamino-2-propen-1-one (650mg, 4.2mmol) and sodium hydroxide (185mg) to give the title compound (90mg) as a white solid m.p. 122°. MS m/z 318 (M+H)⁺. EXAMPLE 18

4-(1 ,2,3.4-Tetrahvdronaphthalen-6-yl)-N- 3.4.5-trimethoxyphenγl)-9- pyrimidineamine from 3,4,5-(trimethoxyphenyl)guanidine nitrate (1 .Og, 435mmol), 3- dimethylamino-1-(1 ,2,3,4-tetrahydronaphthalen-6-yl)-2-propen-1 -one

(801 mg, 3.5mmol) and sodium hydroxide (155mg, 3.85mmol) to give the title compound (120mg) as a yellow solid m.p. 150-151 °. MS m/z 392 (M+H)⁺.

EXAMPLE 19

4-(2.2-Dimethyl-3.4-dihydro-2H-benzorbloxin-fi-vn-N-<3.4,5- trimethoxyphenyh-2-pyrimidineamine from 3,4,5-(trimethoxyphenyl)guanidine nitrate (1 .15g, 4. Ommol) , 3- dimethylamino-1 -(2,2-dimethyl-3,4-dihydro-2H-benzo[b]oxin-6-yl)-2- propen-1 -one (1 .04g, 4. Ommol) and sodium hydroxide (180mg, 4.4mmol) to give the title compound (120mg) as a pale yellow solid m.p. 149-150°. MS m/z 422.3 (M+H)⁺.

EXAMPLE 20 4-rBenzothiazol-2-yn-N-(3.4.5-trimethoxyphenvn-2-pyrimidineamine from 3,4,5-(trimethoxyphenyl)guanidine nitrate (1 .72g, 6. Ommol), 1 - (benzothiazol-2-yl)-3-dimethylamino-2-propen-1 -one (1 .39g, 6. Ommol) and sodium hydroxide (270mg, 6.6mmol) to give the title compound (420mg) as a yellow solid m.p. 182-183°. MS m/z 395 (M+H)+.

EXAMPLE 21

4-(2-Nitrothien-5-vn-N-r3.4.5-trimethoxyphenyl⁾-2- yrimidineamin^p from 3,4,5-(trimethoxyphenyl)guanidine nitrate (1 .27g, 4.4mmol), 3- dimethylamino-1 -(2-nitrothien-5-yl)-2-propen-1 -one (1 .0g, 4.4mmol) and sodium hydroxide (186mg, 4.9mmol) to give the title compound (540mg) as a dark red solid m.p. 182-183°. MS m/z 389 (M+H)+.

EXAMPLE 22

4-(3-Methoxvphenvπ-N- 3.4.5-trimeth^Qyγphenvn-2-pyrimidineaminP from 3,4,5-(trimethoxyphenyl)guanidine nitrate (1 .41 g, 4.88mmol), 3- dimethylamino-1 -(3-methoxyphenyl)-2-propen-1 -one (1 .0g, 4.88mmol) and sodium hydroxide (216mg, 5.4mmol) to give the title compound (275mg) as a yellow solid m.p. 155-156°. MS m/z 368 (M+H)⁺.

EXAMPLE 23 4-(2-Pyridyπ-N-.^'3.4.5-trimethoxy^phenyπ-2-pyrimidineamine from 3,4,5-(trimethoxyphenyl)guanidine nitrate (1 .99g, 6.91 mmol), 3- dimethylamino-1 -(2-pyridyl)-2-propen-1 -one (1 .22g, 6.91 mmol) and sodium hydroxide (276mg, 6.9mmol) to give the title compound (158mg) as a yellow solid m.p. 185°. MS m/z 339 (M+H)⁺.

EXAMPLE 24

4- 4-fer -Butoxycarbonylaminophenyl)-N-(3.4.5-trimethoxyphenyn-2- pyrimidineamine from 3,4,5-(trimethoxyphenyl)guanidine nitrate (10.9g, 37.8mmol), 1 -(4- tet?-butoxycarbbonylaminophenyl)-3-dimethylamino-2-propen-1 -one

(10. Og, 34.4mmol) and sodium hydroxide (1.52g, 37.8mmol) to give the title compound (5.4g) as a yellow solid m.p. 158-159°. MS m/z 453 (M+H)⁺.

EXAMPLE 25

4-f3-Hydroxyphenyπ-N- 3.4.5-trimethoxy^phenvπ-2-pyrimidineamine from 3,4,5-(trimethoxyphenyl)guanidine nitrate (4.72g, 16.4mmol), 1 -(3- fe/?-butyldimethylsilyloxyphenyl)-3-dimethylamino-2-propen-1 -one (5.0g, 16.4mmol) and sodium hydroxide (655mg, 16.4mmol) to give the title compound (2.0g) as a light yellow solid m.p. 191 -192°. MS m/z 354 (M+H)+.

EXAMPLE 26 4-(5-Thiazolvπ-N-⁽3.4.5-trimethoxyphenyl)-2-pyrimidineamine from 3,4,5-(trimethoxyphenyl)guanidine nitrate (634mg, 2.2mmol), 3- dimethylamino-1 -(5-thiazolyl)-2-propen-1 -one (330g, 1 .81 mmol) and sodium hydroxide (88mg, 2.2mmol) to give the title compound (42mg) as a yellow solid m.p. 171°. MS m/z 345 (M+H)⁺. EXAMPLE 27 4-f4-Cvano^phenvn-N-r3.4.5-trimetho^χyphenyn-2-pyrimidineamine from 3,4,5-(trimethoxyphenyl)guanidine nitrate (17.0g, 59. Ommol), 1 -(4- cyanophenyl)-3-dimethylamino-2-propen-1 -one (11.82g, 58. Ommol) and sodium hydroxide (2.36mg, 59. Ommol) to give the title compound (11.66g) as a green solid m.p. 183°. MS m/z 363 (M+H)⁺.

EXAMPLE 28

4-(4-Ethoxycarbonylphenyl)-N-⁽3.4.5-trimethoxyphenyl)-2- pyrimidineamine from 3,4,5-(trimethoxyphenyl)guanidine nitrate (1 1.66g, 40.5mmol), 3- dimethylamino-1 -(4-ethoxycarbonyiphenyl)-2-propen- 1 -one ( 10.0g, 40.5mmol) and sodium hydroxide (1.62g, 40.5mmol) to give the title compound (3.21g) as a yellow solid m.p. 181-182°. MS m/z 410 (M+H)⁺.

EXAMPLE 29

4- 1-Naphthyπ-N-⁽3.4.5-trimethoxyphenyπ-2-^pyrimidineamine from 3,4,5-(trimethoxyphenyl)guanidine nitrate (2,94g, 10.22mmol), 3- dimethylamino-1 -(1-naphthyl)-2-propen-1 -one (2.30g, 10.22mmol) and sodium hydroxide (409mg, 10.22mmol) to give the title compound (750mg) as a yellow solid m.p. 130-133°. MS m/z 444 (M+H)⁺.

EXAMPLE 30

4(4.5-Dimethylthiazol-2-yl)-N-⁽3.4.5-trimethoxyphenvn-2- pyrimidineamine from 3,4,5-(trimethoxyphenyl)guanidine nitrate (4.99g, 6.91 mmol), 3- dimethylamino-1-(4,5-dimethylthiazol-2-yl)-2-propen-1 -one (3.64g, 17.3mmol) and sodium hydroxide (720mg, 18mmol) to give the title compound (490mg) as a yellow solid m.p. 207°. MS m/z 373 (M+H)⁺.

EXAMPLE 31

4-(4-Methoxyphenyn-N-t^'3.4.5-trimethoxyphenvπ-2-pyrimidineamine from 3,4,5-(trimethoxyphenyl)guanidine nitrate (8.16g, 28.3mmol), 3- dimethylamino-1 -(4-methoxyphenyl)-2-propen-1 -one (5.5g, 28.3mmol) and sodium hydroxide (1.13g, 28.3mmol) to give the title compound (3.4g) as an orange solid m.p. 148-150°. MS m/z 368 (M+H).⁺ EXAMPLE 32 4-(4-Benzyloxyphenvn-N-(3.4.5-trimethoxyphenvn-2-pyrimidineamine from 3,4,5-(trimethoxyphenyl)guanιdine nitrate (9.0g, 31 .25mmol), 1 -(4- benzyloxyphenyl)-3-dιmethylamιno-2-propen-1 -one (8.88g, 31 .25mmol) and sodium hydroxide (1 .25g, 31 .25mmol) to give the title compound (1 1.Og) as a yellow solid m.p. 171 -172°. MS m/z 444(M+H)+.

EXAMPLE 33

4-(5-t2-Hvdroxyethvn-4-methylthiazol-2-vn-N-ι3.4.5-trimethQxy- phenyO-2-pyrimidineamine from 3,4,5-(trιmethoxyphenyl)guanidιne nitrate (1 .22g, 4.23mmol), 1 -(5-(2- fett-butyldιmethylsilyloxyethyl)-4-methylthιazol-2-yl)-3-dιmethylamιno-2- propen-1 -one (1.50g, 4 23mmol) and sodium hydroxide (200mg, 5. Ommol) to give the title compound (21 0mg) as an orange solid m.p > 1 90° (decomp). MS m/z 403 (M+H)⁺.

EXAMPLE 34 4-(4-Bromophenyl)-N-(3.4.5-trimethoχyphenyn-2-pyrimidineamine from 3,4,5-(tπmethoxyphenyl)guanιdιne nitrate (2.26g, 7.80mmol), 1 -(4- bromophenyl)-3-dιmethylamιno-2-propen-1 -one (2.0g, 7.80mmol) and sodium hydroxide (346mg, 8.60mmol) to give the title compound (1 .0g) as a green solid m.p. 179°. MS m/z 416 (M+H)+.

EXAMPLE 35 4-f2-Chloropyridin-5-vn-N-(3.4.5-trimethoxyphenvn-2-pyrimidine- amine from 3,4,5-(tπmethoxyphenyl)guanidine nitrate (16.42g, 22.3mmol), 1 -(2- chloropyπdιn-5-yl)-3-dimethylamιno-2-propen-1 -one (4.70g, 22.3mmol) and sodium hydroxide (895mg) to give the title compound (1 .43g) as a yellow solid m.p. 191 -192°. MS m/z 373.2 (M+H)+.

EXAMPLE 36 4-(1-H-lndol-3-yn-N-(3.4.5-trimethoxyphenyn-2-pyrimidinf>aminp

The compound of Example 7 (400mg, 0.78mmol) was suspended in ethanol (5ml) and 4M sodium hydroxide (5ml) and heated at reflux for 4h.

On cooling a precipitate formed which was collected by filtration and washed with water to give the title compound (79mg) as a yellow solid m.p. 197°. MS m/z 377 (M+H)⁺.

EXAMPLE 37 N-Methyl-4-<4-pyridyπ-N- 3.4.5-trimethoxyphenyl)-2-pyrimidineamine A solution of the compound of Example 3 (650mg, 1.92mmol) in DMF (5ml) was added dropwise to a stirred suspension of 60% NaH (76mg, 1.92mmoi) in DMF (5ml), cooled to 0°C. After 0.5h lodomethane (0.13ml, 2.11 mmol) was added dropwise and the resulting mixture was left to warm to room temperature over 20h. The reaction was concentrated under reduced pressure and the resulting residue subjected to column chromatography to give the title compound (159mg) as a pale green solid m.p. 139° MS m/z 353 (M+H)⁺

EXAMPLE 38

4-(2-Aminothien-5-yl)-N-(3.4.5-trimethoxyphenyl)-2-pyrimidineamine

A solution of the compound of Example 21 (380mg, 0.98mmol) was treated with ammonium formate (200mg, 6.34mmol) and 10% palladium on carbon (200mg) at 65° for 24h On cooling, the catalyst was filtered off on a pad of celite and the filtrate concentrated under reduced pressure to give the title compound (100mg) as a green solid m.p. 161 -162° MS m/z 389 (M+H)⁺.

The compounds of Examples 39 and 40 were prepared in a similar manner to the compound of Example 38. EXAMPLE 39 4-ι4-Aminophenyπ-N-«3.4.5-trimethoxyphenvπ-2-pyrimidineamine from the compound of Example 12 (800mg, 2.09mmoi), ammonium formate (660mg, 10.5mmol) and 10% palladium on carbon (80mg) to give the title compound (253mg) as a yellow solid m.p. 195°. MS m/z 353 (M+H)⁺. EXAMPLE 40 4-(3-Aminophenyh-N-(3.4.5-trimetho^χyphenyn-2-pyrimidineamine from the compound of Example 1 1 (150mg, 0.39mmol), ammonium formate (150mg, 2.38mmol) and 10% palladium on carbon (100mg) to give the title compound (120mq) as a vellow solid m p. 166-167° MS m/z 353 (M+H)⁺.

EXAMPLE 41 4-⁽3-Acetamidophenvπ-N-⁽3.4.5-trimethoxyphenyl)-2-pyrimidineamine To a solution of the compound of Example 40 (0.25g, 0.71 mmol) in dry CH2CI2 (40ml) and triethylamine (0.1 1 ml) was added acetyl chloride (0.06ml, 0.78mmol) and the resulting mixture stirred at room temperature for 4h After washing with water (100ml) and saturated aqueous NaHCθ3 (100ml), the organic Iayer was dried (MgSθ₄) and concentrated under reduced pressure. The residue was subjected to column chromatography [silica-ethyl acetate] to give after recrystallisation from ethyl acetate- hexane the title compound (163mα) as a vellow solid, m.p 162-164° MS m z 395(M+H)⁺.

The compounds of Examples 42-44 were prepared in a similar manner to the compound of Example 41 : EXAMPLE 42 4-⁽4-Acetamidophenyh-N-^('3.4.5-trimethoxyphenyl)-2-pyrimidineamine from the compound of Example 39 (300mg, 0.85mmol), triethylamine (0.13ml, 0.94mmol) and acetyl chloride (0.07ml, 0.94mmol) to give the title compound (178mg) as a yellow solid m.p. 239°. MS m/z 395 (M+H)⁺.

EXAMPLE 43

N-ι3- 2- 3.4.5-Trimethoxyphenylamino^)pyrimidin-4-ynphenyl}-5- methylisoxazole-3-carboxamide from the compound of Example 40 (150mg, 0.43mmol) , 5- methylisoxazole-3-carbonyl chloride (68mg, 0.47mmol) and triethyiaminie (0.1ml, 0.7mmol) to give the title compound (7mg) as a yellow solid m.p. 173-175°. MS m/z 462 (M+H)⁺. EXAMPLE 44

N-(3- 2-t3.4.5-Trimet oxyp enylaminoιpyrimidin-4-ynphenvnpyridine-

3-carboxamide from the compound of Example 40 (250mg, 0.71 mmol), nicotinoyl chloride hydrochloride (139mg, 0.78mmol), and triethylamine (0.2ml, 2.3mmol) to give the title com^pound (155mg) as a yellow solid m.p. 140-143°. MS m/z 458 (M+H)⁺.

EXAMPLE 45 4-ι3-⁽3-Phthalimidopropoxy⁾phenyl>-N-r3.4.5-trimethoχyphenyl)-2- pyrimidineamine

To a suspension of caesium carbonate (462mg, 1.4mmol) in dry DMF (10ml) under a nitrogen atmosphere were added the compound of Example 25 (500mg, 1.4mmol) and 3-bromopropylphthalimide (380mg, 1.4mmol). The resulting mixture was stirred at room temperature for 4.5h, followed by the addition of water (50ml). The resulting precipitate was collected, washed with diethyl ether and recrystallised from ethyl acetate- hexane to give the title compound (620mg) as an off-white solid m.p. 209°. MS m/z 541 (M+H)⁺.

The following compound was prepared in a similar manner: EXAMPLE 46

4-⁽3-Pro^poχy^phenyπ-N-⁽3.4.5-trimethoxyphenyl⁾-2-^pyrimidineamine from the compound of Example 25 (350mg, 0.99mmol), iodopropane (168mg, 0.99mmol) and caesium carbonate (322mg, 0.99mmol) to give the title compound (187mg) as an off-white solid m.p. 107°. MS m/z 396 (M+H)⁺.

EXAMPLE 47 4-ι3- 2-Hvdroxyethoxy phenvπ-N-f3.4.5-trimethoxyphenyl>-2- pyrimidineamine

To a suspension of 60% NaH (dispersion in oil) (62mg, 1.6mmol) in DMF (15ml) under a nitrogen atmosphere were added the compound of Example 25 (500mg, 1 .4mmol) and ethylene carbonate (137mg, 1.6mmol). The resulting mixture was heated at 130° for 6h. On cooling the reaction was diluted with water (30ml), extracted with CH₂CI₂ (3x25ml), dried (MgSθ4) and concentrated under reduced pressure. The resulting residue was subjected to column chromatography [silica-ethyl acetate] to give the title compound (260mg) as a yellow solid m.p. 70°. MS m/z 398 ( +H)⁺.

EXAMPLE 48

4-(3-(3-Aminopropoxy henvπ-N-(3.4.5-trimethoxyphenvπ-2- pyrimidineamine dihydrochloride

The compound of Example 45 (500mg, 0.9mmol) was suspended in ethanol (30ml) containing hydrazine monohydrate (0.14ml, 2.8mmol) and the resulting mixture heated at reflux for 18h. On cooling the resulting precipitate was filtered off and the filtrate concentrated under reduced pressure. The residue was suspended in CH2CI2 (30ml), extracted with 2N hydrochloric acid (2x30ml) Combined acid layers were taken to pH 1 1 with 6N NaOH and extracted with CH CI (3x30ml), the organic layers were dried (MgS04), and concentrated under reduced pressure. The residue was dissolved in ethanol (10ml) and the solution saturated with HCl (g). The resulting precipitate was collected to give the title compound (137mg) as an orange solid m.p. 236°. δH (d6-DMS)0 9.62 (1 H, s), 8.54 (1 H, d, J 5.2Hz), 8.05 (2H, br s), 7.75 (2H, m), 7.45 (2H, m), 7.29 (2H, s), 7.14 (1 H, d, J 7.3Hz), 4.14 (2H, t, J 6.0Hz), 3.79 (6H, s), 3.62 (3H, s), 2.95 (2H, m) and 2.06 (2H, m). MS m/z 41 1 (M+H)⁺.

EXAMPLE 49 4-(4-Hvdroxyphenvn-N-t3.4.5-trimethoχyphenvπ-2-pyrimidineamine

A solution of the compound of Example 32 (1 .06g, 2.37mmol) in ethanol (100ml) was treated with ammonium formate (750mg, 12. Ommol) and 10% palladium on carbon (100mg) and stirred at room temperature for 18h, followed by heating at reflux for 18h. On cooling the catalyst was removed by filtration through Celite® and the filtrate concentrated under reduced pressure. The resulting residue was recrystallised from ethyl acetate to give the title compound (760mg) as a cream solid m.p. 200-202°. MS m/z 354 (M+H)⁺. EXAMPLE 50

4-f4-t3-Phthalimidopropoxy⁾phenyn-N-⁽3.4.5-trimethoχvphenyn-2- pyrimidineamine

To a supension of 60% NaH (dispersion in oil) (55mg, 1.36mmol) in DMF (10ml) was added the compound of Example 49 (480mg, 1.36mmol) and 3-bromopropylphthalimide (365mg, 1.36mmol). The resulting mixture was stirred at room temperature for 6h. After this time the reaction was concentrated under reduced pressure, the residue dissolved in ethyl acetate (50ml), washed with water (50ml), and dried (MgSθ₄). The solvent was removed under reduced pressued to give the title compound (700mg) as a buff solid. MS m/z 541 (M+H)+.

EXAMPLE 51

4- 4-⁽3-Aminopropoxy)phenvn-N-⁽3.4.5-trimethoχyphenyn-2- pyrimidineamine

The compound was prepared in a manner analogous to the preparation of the compound of Example 48 from the compound of Example 50 (830mg, 1.54mmol) and hydrazine monohydrate (0.23ml, 4.62mmol) to give the title compound (63mα) as a white solid m.p. 161 -162°. MS m/z 41 1 (M+H)⁺.

EXAMPLE 52

4-ι4-⁽2-Hvdroxyethoxy^)phenyn-N-⁽3.4.5-trimethoxyphenyn-2- pyrimidineamine

The compound was prepared in a manner analogous to the preparation of the compound of Example 47 from the compound of Example 49 (250mg, 0.71 mmol), ethylene carbonate (70mg, 6.78mmol) and 60% sodium hydride (dispersion in oil) (30mg, 0.78mmol) to give the title compound (140mg) as a yellow solid m.p 145-146°. MS m/z 398 (M+H)⁺.

EXAMPLE 53

4-⁽4- 2-N.N-Dimethylaminoethoxy⁾Dhenyn-N-ι3.4.5-trimethoxyphenyn- 2-pyrimidineamine

The compound of Example 49 (250mg, 0.71 mmol), N,N-dimethylamιno- ethanol (69mg, 0.78mmol) and triphenylphosphine (205mg, 0.78mmol) were dissolved in dry THF (20ml) under a nitrogen atmosphere and the mixture stirred for 0.5h. Diethylazodicarboxylate (136mg, 0.78mmol) in THF (10ml) was added dropwise and the reaction stirred for 48h, after which time the solvent was removed under reduced pressure. The residue was dissolved in ethyl acetate (40ml), washed with water (2x100ml), dried (MgS04) and the solvent evaporated. The resulting solid was subjected to column chromatography [silica-ethyl acetate] to give the title compound (75mg) as white crystals m.p. 140-147°. MS m/z 425 (M+H)⁺.

The foilowing compound was prepared in a similar manner: EXAMPLE 54

4- 4- 3-N.N-Dimethylaminopropoxy)phenvn-N-(3.4.5-trimethoxy- phenyl)-2-pyrimidineamine from the compound of Example 49 (250mg, 0.71 mmol), N,N- dimethylaminopropanol (88mg, 0.85mmol), triphenylphosphine (223mg, 0.85mmol) and diethylazodicarboxylate (148mg, 0.85mmol) to give the title compound (20mq) as a white solid m.p. 161 -164°. MS m/z 439 (M+H)+.

EXAMPLE 55 4-ι3- 3-Hvdroxypropylamino⁾phenyl⁾-N-⁽3.4.5-trimethoxyphenyπ-2- pyrimidineamine

The compound of Example 40 (250mg, 0.71 mmol) and 3-bromopropanol were heated at 85° in DMF for 72h. The solvent was removed under reduced pressure and the residue subjected to column chromatography [silica-ethyl acetate] to give the title compound (33mg) as a yellow solid m.p. 92-95°. MS m/z 411 (M+H)⁺.

The following compound was prepared in a similar manner: EXAMPLE 56

4-⁽4-t3-Hvdroxypropylamino⁾phenyπ-N-₁3.4.5-trimethoxyphenvn-2- pyrimidineamine from the compound of Example 39 (750mg, 2.13mmol) and 3- bromopropanol (3.55mg, 2.56mmol) to give the title compound (24mg) as a yellow solid m.p. 86-90°. MS m/z 411 (M+H)+. EXAMPLE 57

4-(3-(3-Pyridylmethvhaminophenyl)-N-t3.4.5-trimethoxyphenyl)-2- pyrimidineamine

To a solution of the compound of Example 40 (250mg, 0.71 mmol) in CH2CI2 (20ml) was added 3-pyridinecarboxaldehyde (69mg, 0.65mmol), sodium triacetoxyborohydride (226mg, 1.07mmol) and acetic acid (0.1 ml), and the resulting mixture stirred at ambient temperature for 48h. The reaction was washed with water (2x100ml), dried (MgSθ₄), concentrated under reduced pressure, and the residue columned [silica-ethyl acetate] to give the title com^pound M OOmg. as a yellow solid m p 7R-ftno MS m/z 444 (M+H)+.

The following compound was prepared in a similar manner: EXAMPLE 58 4-⁽4-ι3-Pyridylmethvnaminophenvn-N-t3.4.5-trimethoχphenyn-2- pyrimidineamine from the compound of Example 39 (250mg, 0.71 mmol), 3-pyridine- carboxaldehyde (69mg, 0.65mmol), sodium triacetoxyborohydride (226mg, 1.67mmol) and acetic acid (0.1 ml) to give the title compound (134mg) as a yellow solid m.p. 189-190°. MS m/z 444 (M+H)+.

EXAMPLE 59

4-⁽4-ιAminomethyl^)phenvπ-N-₁3.4.5-trimethoxyphenyl)-2- pyrimidineamine To a suspension of L.AIH4 (628mg, 16.56mmol) in dry THF (100ml) at 0° was added dropwise a solution of the compound of Example 27 in THF (10ml) and when addition was complete the reaction was heated at reflux for 3h. On cooling a saturated solution of aqueous ammonium chloride (25ml) was added and the organic solvent removed under reduced pressure. The aqueous solution was extracted with ethyl acetate (2x200ml) and the combined organic layers dried (MgSθ₄) followed by evaporation under reduced pressure. The residue was subjected to column chromatography [silica-ethyl acetate] to give the title compound (330mg) as a yellow solid m.p. 143-144°. MS m/z 367 (M+H)+. EXAMPLE 60

4./4-π H-1.2.3.4-Tetrazol-5-vnphenvh-N-(3,4.5-trimethoχyphenyl)-2- pyrimidineamine To a solution of the compound of Example 27 (500mg, 1.38mmol) in DMF (15ml) was added sodium azide (897mg, 13.8mmol) and ammonium chloride (738mg, 13 δmmol) and the mixture heated at 130° for 18h On cooling, water (45ml) was added, the resulting precipitate collected and recrystallised from ethyl acetate to give the title compound (333mg) as a yellow solid m.p 246-247° δH 9.60 (1 H, br s), 8 59 (1 H, d, J 5 OHz), 8 39 (2H, d, J 8 4), 8.19 (2H, d, J 8 4Hz), 7 47 (1 H, d, J 5 3Hz), 7 31 (2H, s), 3 80 (6H, s) and 3 62 (3H, s) MS m/z 406 2 (M+H)⁺

EXAMPLE 61 4-(1-Oxopyrid-4-yl)-N-f3.4.5-trimethoxyphenyl)-2-pyrimidineamine

In a manner analogous to the preparation of the compound of Example 1 , from 3,4,5-trιmethoxyphenyiguanιdιne nitrate (2 25g, 7 81 mmol), 3- dιmethylamιno-1 -(1 -oxo-pyπd-4-yl)-2-propen-1 -one (880mg, 7 81 mmol) and sodium hydroxide (344mg, 8.6mmol) to give the title compound (1 2g) as a green solid m p. 220° MS m/z 355 (M+H)⁺

The 3-dιmethylamιno-1 -(1 -oxopyrιd-4-yl)-2-propen-1 -one used as starting material was prepared by heating a solution of 4-acetylpyπdιne-N-oxιde (2.5g, 18.2mmol) in dimethylformamide diethylacetal (30ml) at reflux for 0 5h On cooling the resulting solid was collected and washed with diethyl ether to give the desired product (3.18g) as an orange solid m p 181 °

The 4-acetylpyπdιne-N-oxιde was prepared by treating a solution of 4- acetylpyridme (3.0g, 24 8mmol) in CH₂CI₂ with 3-chloro-peroxybenzoιc acid [Aldrich 57-86%] (8 4g) at room temperature for 12 h The reaction was filtered, the filtrate concentrated under reduced pressure and the resulting residue subjected to column chromatography [silica 10% methanol-ethyl acetate] to give the desired product (3.2g) as a white solid m.p. 101 ° EXAMPLE 62

4-(4-< Hydroxymethy phenvn-N-r3.4.5-trimethoxyphenyl)-2- pyrimidineamine

To a suspension of L.AIH4 (560mg, 14.64mmol) in dry THF (50ml) at 0° was added dropwise to a solution of the compound of Example 28 in THF (100ml) and the reaction heated at reflux for 18h. On cooling an aqueous saturated solution of ammonium chloride (70ml) was added and the organic solvent removed under reduced pressure. The aqueous solution was extracted with CH CI₂ (300ml), and this was dried (MgS04) and evaporated under reduced pressure. The residue was subjected to column chromatography [silica-ethyl acetate] to give title compound (212mg) as a pale yellow solid m.p. 171 -172°. MS m/z 368 (M+H)⁺.

EXAMPLE 63 4-t^'2-f3-Hvdroxypropylamino)pyridin-5-vn-N-^('3.4.5-trimethoxyphenyn- 2-pyrimidineamine

The compound of Example 35 (200mg, 0.54mmol) and 3-amιno-1 - propanol (1 .0ml, 13.05mmol) were heated at 100° for 3.5h. On cooling the reaction was concentrated under reduced pressure, water (15ml) added and the resulting precipitate collected. After washing with water, drying under vacuum and recrystallisaiton from ethanol the title compound (105mg) was obtained as a yellow solid m.p. 168-160°. δ H(d⁶ DMSO) 9.40 (1 H, s), 8.83 (1 H, s), 8.38 (1 H, d, J 5.3Hz), 8.12 (1 H, br dm, J 7.0Hz), 7.26 (2H, s), 7.22 (1 H, d, J 5.2Hz), 7.16 (1 H, br t, J 3.0Hz), 6.54 (1 H, d, J 8.8Hz), 4.53 (1 H, t, J 5.0Hz), 3.76 (6H, s), 3.60 (3H, s), 3.47 (2H, q, J 5.4Hz), 3.32 (2H, m) and 1 .68 (2H, m). MS m/z 412 (M+H)+.

The compounds of Examples 64-87 were prepared in a similar manner to the compound of Example 63 using the compound of Example 35 and the amine shown:

EXAMPLE 64

4-<'2-t^'2-Aminoethylamino)Pyridin-5-yl⁾-N-f3.4.5-trimethoxyphenyl)-2- pyrimidineamine from the compound of Example 35 (200mg, 0.54mmol) and ethylene- diamine (1 ml, 14.96mmoh to give the title compound (105mg) as a yplln solid m.p. 1 17-1 18° δH(d⁶ DMSO) 9.35 (1 H, s), 8.83 (1 H, d, J 2.2Hz), 8.38 (1 H, d, J 5.3Hz), 8.13 (1 H, dd, J 8.9, 2.4Hz), 7.27 (2H, s), 7.22 (1 H, d, J 5.3Hz), 7.1 1 (1 H, br t, J 5.0Hz), 3.77 (6H, s), 3.62(3H, s), 3.29 (2H, q, J 6.3Hz), 2.71 (2H, t, J 6.3Hz) and 1.60 (2H, br s). MS m/z 397 (M+H)+.

EXAMPLE 65

4-_t2-⁽2-Hvdroxyethylamino^)pyridin-5-vn-N-r3.4.5-trimetho^χyphenyn-2- pyrimidineamine from the compound of Example 35 (300mg, 0.81 mmol) and ethanolamine (1.0ml, 16.56mmol) to give the title compound (190mg) as an off-white solid m.p. 123°. δH(d6 DMSO) 9.35 (1 H, s), 8.82 (1 H, d, J 2.2Hz), 8.38 (1 H, d, J 5.3Hz), 8.12 (1 H, dd, J 8.8, 2.4Hz), 7.26 (2H, s), 7.22 (1 H, d, J 6.3Hz), 7.1 1 (1 H, br t, J 5.0Hz), 6.59 (1 H, d, J 8.8Hz), 4.73 (1 H, br s), 3.77 (6H, s), 3.62 (3H, s), 3.57-3.52 (2H, m) and 3.45-3.38 (2H, m). MS m/z 398 (M+H)⁺.

EXAMPLE 66

4-⁽2-(3-Aminopropylamino)pyridin-5-vn-N-⁽3.4.5-trimethoxyphenvn-2- pyrimidineamine from the compound of Example 35(250mg, 0.68mmol) and 1 ,3-dιamιno- propane (1.0ml, 1 1.89mmol) to give the title compound (120mg) as a yellow solid m.p. 152-153° δH(d⁶ DMSO) 9.34 (1 H, s), 8.83 (1 H, d, J 2.2Hz), 8.37 (1 H, d, J 5.3Hz), 8.12 (1 H, dd J 8.8, 2.4Hz), 7.26 (2H, s), 7.21 (1 H, d, J 5.3Hz), 7.14 (1 H, bt, J 5.5Hz), 6.53 (1 H, d, J 8.8Hz), 3.77 (6H, s), 3.62 (3H, s), 3.34 (2H, q, J 7.0Hz), 2.61 (2H, t, J 6.7 Hz), 2.40 (2H, br s) and 1.65-1.56 (2H, m). MS m/z 411 (M+H)+.

EXAMPLE 67

4-(2-f2-Dimethylaminoethylamino^)pyridin-5-yπ-N-(3.4.5-trimethoxy- phenyl)-2-pyrimidineamine from the compound of Example 35(250mg, 0.68mmol) and N,N-dimethyl- ethylenediamine (0.75mol, 14.17mmol) to give the title compound (85mg) as an off-white solid m.p. 110-11 1 °. MS m/z 425.5 (M+H)+. EXAMPLE 68

4-⁽2-⁽4-Hvdroxybutylamino⁾pyridin-5-vπ-N-⁽3.4.5-trimethoxyphenvh-2- pyrimidineamine from the compound of Example 35(250mg, 0.68mmol) and 4-amιno-1 - butanol (0.750mg, 8.43mmol) to give the title compound (255mg) as a yellow solid m.p. 170-171°. MS m/z 4216 (M+H)+.

EXAMPLE 69

4-(2-(2-<'Pyrrolidin-1-vπethylamino⁾pyridin-5-vπ-N-(3.4.5-trimethoxy- phenyl)-2-pyrimidineamine from the compound of Example 35 (250mg, 0.68mmol) and N-(2-amιno- ethyl)pyrrolidιne (0.75ml, 7.31 mmol) to give the title compound (191 mg) as a yellow solid m.p. 165-166°. MS m/z 451 (M+H)⁺.

EXAMPLE 70

4-ι2-f2-Methylamino)ethyl(methvπamino)pyridin-5-yπ-N-ι3.4.5- trimethoxyphenyl)-2-pyrimidineamine from the compound of Example 35 (300mg, 0.81 mmol) and N,N'- dimethylethylenediamme (0.61ml, 5.68mmol) to give the title compound (60mg) as a buff solid m.p. 115-1 16°. MS m/z 425 (M+H)⁺.

EXAMPLE 71

4- 2- 3-lsopropylaminopropylamino⁾pyridin-5-vn-N-<'3.4.5-trimethoxy- phenyl)-2-pyrimidineamine from the compound of Example 35 (300mg, 0.81 mmol) and N-isopropyl- 1 ,3-propanediamιne (0.51ml, 4. Ommol) to give the title compound (208mg) as a pale yellow solid m.p. 124-125°. MS m/z 453 (M+H)⁺.

EXAMPLE 72 4-(2-f1-Benzylpiperid-4-ylamino)pyridin-5-yπ-N-ι3.4.5-trimethoxy- phenyl)-2-pyrimidineamine from the compound of Example 35 (500mg, 1.35mmol) and 4-amιno-1 - benzylpiperidine (514mg, 2.7mmol) to give the title compound (370mg) as a yellow solid m.p. 113-114°. MS m/z 527 (M+H)+. EXAMPLE 73

4-(2-(3- Morpholino propylamino yridin-5-vh-N-(3.4.5- trimethoxyphenyl)-2-pyrimidineamine from the compound of Example 35(300mg, 0.81 mmol) and 3- (aminopropyl)morpholine (0.59ml, 4mmol) to give the title compound (300mg) as a white solid m.p. 165-166°. MS m/z 481 (M+H)⁺.

EXAMPLE 74

4-ι2-ι4-Methoxyphenylamino)pyridin-5-yl)-N-(3.4.5-trimethoxy- phenvπ-2-pγrimidineamine from the compound of Example 35 (300mg, 0.81 mmol) and para-anisidine (200mg, 1.62mmol) to give the title compound (170mg) as a yellow solid m.p. 209-210°. MS m/z 459 (M+H)⁺.

EXAMPLE 75

4-⁽2-^(,3-Methylamino^propyl⁽methvnamino⁾pyridin-5-yl)-N-t3.4.5- trimethoxyphenyh -2-pyrimidineamine

In a manner analogous to Example 63 from the compound of Example 35 (500mg) and N,N'-dimethyl-1 ,3-propanediamine (1 .62ml. 13. Ommol) to give the title com^pound ⁽31 mα⁾ as a vellow solid, m.p. 103°. MS m/z 439 (M+H)⁺.

EXAMPLE 76

4-(2-f2-Hvdroxycvclohexylamino yridin-5-yl⁾-N-(3.4.5-trimethoxy- phenyπ-2-pyrimidineamine from the compound of Example 35 (300mg, 0.81mmol), trans-2-amino- cyclohexanol hydrochloride (614mg, 4.05mmol) and triethylamine (0.56ml, 4.05mmol) to give the title compound (147mg) as a yellow solid m.p. 147- 148°. MS m/z 452 (M+H)+.

EXAMPLE 77

4-r2-ι3-Dimethylamiπo-2.2-dimethylpropylamino^)pyridin-5-vn-N-ι3.4.5- trimethoxyphenyπ-2-Pyrimidineamine from the compound of Example 35 (300mg, 0.81 mmol) and N,N,2,2- tetramethyl-1 , 3-propanediamine (527mg, 4.05mmol) to give the title compound (147mg) as a white solid m.p. 125°. MS m/z 467 (M+H)⁺. EXAMPLE 78

4-f2-(3-Dimethylaminopropylamino^pyridin-5-yπ-N-<'3.4₁5- trimethoxyphenyπ-2-pyrimidine from the compound of Example 35 (300mg, 0.81 mmol) and 3-dιmethyl- aminopropylamine (248mg, 2.43mmol) to give the title compound (201 mg) as a buff solid m.p. 170°. MS m/z 439 (M+H)+.

EXAMPLE 79 4-⁽2-f2-Diethylaminoethyl(methvπamino ^pyridin-5-v0-N-(3.4.5- trimethoxyphenvn-2-pyrimidine from the compound of Example 35 (300mg, 0.81 mmol) and N.N-dιethyl-N'- methylethylenediamme (316mg, 2.43mmol) to give the title compound

(372mg) as a buff solid m.p. 1 11 °. MS m/z 467 (M+H)⁺.

EXAMPLE 80

4-(2-(3-Diethylaminopropylamino)pyridin-5-yh-N-f3,4.5-trimethoxy- phenyl)-2-pyrimidineamine from the compound of Example 35 (300mg, 0.81 mmol) and N,N-dιethyl- ammopropylamine (282mg, 2.43mmol) to give the title compound (146mg) as a yellow solid m.p. 67°. MS m/z 453 (M+H)⁺.

EXAMPLE 81

4- 2-f2-⁽1-Methylpyrrolidin-2-yl ethylamino)^pyridin-5-vn-N-f3.4.5- trimethoxγphenvO-2-pyrimidineamine from the compound of Example 35 (300mg, 0.81 mmol) and 2-(2- aminoethyl)-1 -methylpyrrolidine (512mg, 4. Ommol) to give the title compound (190mg) as a yellow solid m.p. 176-177°. MS M/z 465 (M+H)+

EXAMPLE 82

4-ι2-π-(Ethylpyrrolidin-2-yhmethylamino^pyridin-5-yl)-N-(3.4.5- trimethoxy-phenyh-2-pyrimidineamine from the compound of Example 35 (300mg), 0.81 mmol) and 2-(amιno- methyl)-1 -ethylpyrrolidine (5.3mg, 4. Ommol) to give the title compound (240mg) as a yellow solid m.p. 81-82°. MS m/z 465 (M+H)⁺. EXAMPLE 83

4- 2-f2-Dimethylaminoethylfethyπamino)pyridin-5-yl)-N-(3.4.5- trimethoxyphenyO-2-pyrimidineamine. from the compound of Example 35(300mg, 0.81 mmol) and N,N-dimethyl- N'-ethylethylenediamine (282mg, 2.43mmol) to give the title compound (193mg) as a yellow solid, m.p. 90°. MS m/z 453 (M+H)+.

EXAMPLE 84 4-(2-(4-Aminobutylamino⁾pyridin-5-yl)-N-(3.4.5-trimethoxyphenyl)-2- pyrimidineamine from the compound of Example 35 (300mg, 0.81 mmol) and 1 ,4-butane- diamine (705mg, 8. Ommol) as a yellow solid m.p. 209-201 °. δ H(d⁶ DMSO) 9.35 (1 H, s), 8.82 (1 H, d, J 2.3Hz), 8.37 (1 H, d, J 5.3Hz), 8.1 1 (1 H, dd, J 8.9, 2.3Hz), 7.26 (2H, s), 7.21 (1 H, d, J 5.3Hz), 7.15 (1 H, br t, NH), 6.53 (1 H, d, J 8.9Hz), 3.77 (6H, s), 3.61 (3H, s), 3.28 (2H, q, J 6.0Hz), 2.58 (2H, t, J 6.9Hz), 1.58-1.53 (2H, m) and 1.97-1.39 (2H, m). MS m/z 425 (M+H)⁺.

EXAMPLE 85 4-(2-(2-Diethylaminoethylamino)pyridin-5-yl)-N-(3.4.5-trimethoxy- phenyl)-2-pyrimidineamine from the compound of Example 35 (300mg, 0.81 mmol) and N,N-diethyl- ethylenediamine (282mg, 2.43mmol) to give the title compound (50mg) as a yellow solid m.p. 67°. MS m/z 453 (M+H)+.

EXAMPLE 86

4-⁽2-ι3-ι4-Methylpiperazin-1-yπpropylamino⁾pyridin-5-yl)-N-f3.4.5- trimethoxyphenyD-2-pyrimidineamine from the compound of Example 35 (350mg, 0.94mmol) and 1 -(3- aminopropyl)-4-methylpiperazine (629mg, 4. Ommol) to give the title compound (238mg) as a white solid m.p. 183-184°. MS m/z 494 (M+H)+. Example 87

4-f2-ι2-Dimethylaminoethylιmethyhamino⁾pyridin-5-ylι-N-(3₁4₁.S- trimethoxypheπyl..pyrimidine-2-amine from the compound of Example 35 (30mg, 0.81 mmol) and N.N.N'- trimethylethylenediamine (400mg, 4. Ommol) to give the title compound (31 Og) as a yellow solid m.p. 97-98°. MS m/z 439 (M+H)⁺.

EXAMPLE 88

4-f2-⁽Piperid-4-ylamino⁾pyridin-5-vn-N-(3.4.5-trimethoxyphenyl)-2- pyrimidineamine acetate

To a solution of the compound of Example 72 (90mg, 0.15mmol) as the acetate salt in ethanol (10ml) was added cyclohexadiene (0.095ml, LOmmol) and 10% palladium on charcoal (I 00mg) and the reaction heated at reflux for 1 h. On cooling the reaction was filtered through a pad of Celite® and the filtrate concentrated under reduced pressure to give the title com^pound (20mg) as a yellow solid m.p.87-88°. MS m/z 437 (M+H)⁺.

EXAMPLE 89 N4-Phenyl-N2-⁽3.4.5-trimethoxy^phenvn-2.4-^pyrimidinediamine

A solution of N-phenyl-2-chloro-4-pyrimidineamιne (1.83g, 8.91 mmol) and 3,4,5-trimethoxyaniline (1.83g, 10. Ommol) in acetone (10ml) and water (15ml) containing concentrated hydrochloric acid (0.2ml) was heated at reflux for 10h. On cooling the acetone was removed under reduced pressure, and the reaction taken to pH10 with 4N NaOH and extracted with ethyl acetate (2x75ml). The combined organic layers were dried (MgSθ₄), concentrated under reduced pressure and the residue crystallised from ethyl acetate to give the title compound (2.41 g) as a colourless solid m.p. 191 °. δH (CDCI3) 8.04 (1 H, d, J 5.8Hz), 7.37-7.34 (4H, m), 7.16-7.10 (1 H, m), 7.05 (1 H, br s), 6.85 (2H, s), 6.68 (1 H, br s), 6.16 (1 H, d, J 5.8Hz), 3.82 (3H, s) and 3.81 (6H, s). MS m/z 353 (M+H)+. The pyrimidineamine used as starting material was prepared by heating a solution of 2,4-dichloropyrimidine (4.0g, 26.8mmol), aniline (2.46ml, 27. Ommol) and triethylamine (4.3ml, 30. Ommol) in ethanol (50ml) at reflux for 2h. On cooling a white precipitate was collectd which was washed with cold ethanol (100ml) and recrystallised from ethyl acetate to give the desired product (6.01 g) as a colourless solid m.p. 186°. MS/mz 206 (M+H)⁺.

The compounds of Examples 90-110 were prepared in a similar manner to the compound of Example 89 using the starting material shown. In each case the pyrimidineamine starting material was prepared from the available compounds shown in a similar manner to the pyrimidineamine starting material of Example 89: EXAMPLE 90 N4-(4-(2-Hvdroxyethoxy)phenvn-N2-(3.4.5-trimethoxyphenyn2.4- pyrimidineamine from 2-chloro-N-(4-(2-hydroxyethoxy)phenyl)-4-pyrimidineamine (187mg, 0.70mmol) and 3,4,5-trimethoxyaniline (146mg, O.θmmol) to give the title compound (18mg) as a colourless solid m.p. 212°. δH (d⁶ DMSO) 9.09 (1 H, br s), 8.89 (1 H, br s), 7.94 (1 H, d, J 5.8Hz), 7.53 (2H, d, J 8.6Hz), 7.10 (2H, s), 6.86 (2H, d, J 8.6Hz), 6.10 (1 H, d, J 5.8Hz), 4.83 (1 H, t, J 5.6Hz),. 3.95 (2H, t, J 4.9Hz), 3.71 -3.68 (2H, m), 3.65 (6H, s) and 3.60 (3H,s). MS m/z 413 (M+H)⁺. The pyrimidineamine starting material was prepared from 4-(2-hydroxy- ethoxy)aniline (481 mg, 3.14mmol) 2,4-dichloropyrimidine (468mg, 3.14mmol) and triethylamine (0.46ml, 3.25mmol) to give the desired product as a yellow solid m.p. 169°. MS m/z 266(M+H)+.

EXAMPLE 91 N4-(3.4-Methylenedioxγphenvπ-N2-⁽3.4.5-trimethoχyphenγn-2.4- pyrimidinediamine from 2-chloro-N-(3,4-methylenedioxyphenyl)-4-pyrimidineamine (200mg, 0.85mmol) and 3,4,5-trimethoxyaniline (156mg, 0.85mmol) to give the title compound (187mg) as a buff solid m.p. 199°. MS m/z 397 (M+H)+. The pyrimidineamine starting material was prepared from 3,4- methylenedioxyaniiine (219mg, 1.60mmol) 2,4-dichloropyrimidine (250mg, 1.60mmol) and triethylamine (0.25ml, 1 .80mmol) to give the desired product (215mg) as a buff solid m.p. 148°. MS m/z 250 (M+H)+. EXAMPLE 92

N4-⁽3-Methoxyphenvn-N2-(3.4.5-trimethoxyphenvn-2.4-pyrimidine- diamine from 2-chloro-N4-(3-methoxyphenyl)-4-pyrιmidineamιne (195mg, 0.83mmol) and 3,4,5-tπmethoxyanιiine (152mg, 0.83mmol) to give the title compound (189mg) as a white solid m.p. 78°. MS m/z 383 (M+H)+. The pyrimidineamine starting material was prepared from meta-anisidme (0.18mg, 1 .6mmol), 2,4-dichloropyrιmιdιne (250mg, 1 .6mmol) and triethylamine (0.25mg, 1.8mmol) to give the desired product (200mg) as a white solid m.p. 107°. MS m/z 236 (M+H)⁺.

EXAMPLE 93

N4- 3-⁽2-Hydroxyethvn^phenvn-N2-f3.4.5-trimethoχyphenyn-2.4- pyrimidinediamine from 2-chloro-N-(4-(2-hydroxyethyl)phenyl)-4-pyπmιdιneamιne (250mg, LOmmol) and 3,4,5-trιmethoxyanιlιne (184mg, LOmmol) to give the title compound (257mg) as a white solid m.p. 72°. MS m/z 397 (M+H)+. The pyrimidineamine starting material was prepared from 2-(4-amιno- phenyl)ethanol (219mg, 1.6mmol) 2,4-dιchloropyrιmιdιne (250mg, 1.6mmol) and triethylamine (0.25ml, 1.8mmol) to give the desired product (289mg) as an orange solid m.p. 163°.

EXAMPLE 94

N4-(4-(Diethylaminoϊphenyπ-N2-(3.4.5-trimethoxγphenvπ-2.4- pyrimidinediamine from 2-chloro-N-(4-(diethylamιno)phenyl)-4-pyπmidineamιne (250mg, 0.9mmol) and 3,4,5-trimethoxyaniiine (166mg, 0.9mmol) to give the title compound (80mg) as a green solid m.p. 87° MS m/z 424.5 (M+H)⁺. The pyrimidineamine starting material was prepared from N,N-dιethyl-1 ,4- phenyldiamine (263mg, 1.6mmol), 2,4-dιchloropyrιmιdιne (250mg, 1.6mmol) and triethylamine (0.25ml, Lθmmol) to give the desired prdocut (410mg) as a green solid m.p. 212°. MS m/z 277 (M+H).+ EXAMPLE 95

N4-(4-ι3-Hvdroxypropoxy)phenyπ-N2-(3.4.5-trimethoχyphenyh-2.4- pyrimidinediamine from 2-chloro-N-(4-(3-hydroxypropoxy)phenyl)-4-pyrimidiπeamine (250mg, 0.89mmol) and trimethoxyaniline (164mg, 0.89mmol) to give the title compound (61 mg) as a white solid m.p. 69°. MS m/z 427 (M+H)⁺. The pyrimidineamine starting material was prepared (from 4-(3-hydroxy- propoxy)aniline (267mg, 1 .60mmol), 2,4-dichloropyrimidine (250mg, LδOmmol) and triethylamine (0.25ml, 1 .80mmol) to give the desired product (383mg) as a pale orange solid m.p. 181 °. MS m/z 280 (M+H)⁺.

EXAMPLE 96

N4-(3,4-Dimethoxyphenyl)-N2-(3,4,5-trimethoxyphenyl)-2.4- pyrimidinediamine from 2-chloro-N-(3,4-dimethoxyphenyl)-4-pyrimidineamine (250mg) and 3,4,5-trimethoxyaniline (189mg, LOmmol) to give the title compound (160mg) as a light pink solid m.p. 86°. MS m/z 413 (M+H)⁺. The pyrimidineamine starting material was prepared from amiπoveratrole (257mg, 1 .60mmol) , 2,4-dichloropyrimidine (2.50mg, 1 .60mmol) and triethylamine (0.25ml, 1 .80mmol) to give the desired product (357mg) as a purple solid m.p. 104° MS m/z 266 (M+H)L

EXAMPLE 97

N4-(4-(4-Methylpiperazin-1-v0phenylVN2-⁽3.4.5-trimethoxyphenyl)- 2.4- pyrimidinediamine from 2-chloro-N-(4-(4-methylpiperazin-1 -yl)phenyl)-4-pyrimidineamine (1 10mg, 0.40mmol) and 3,4,5-trimethoxyaniiine (66mg, 0.40mmol) to give the title compound (80mg) as a white solid m.p. 104°. MS m/z 451 (M+H)+. The pyrimidineamine starting material was prepared from 4-(4- methylpiperazin-1 -yl)aniline (51 1 mg, 2.67mmol), 2,4-dichloropyrimidine (398mg, 2.67 mmol) and triethylamine (I0.4ml, 2.94mmol) to give the desired product (1 10mg) as a white solid m.p. 122°. EXAMPLE 98

N4-(3.5-Dimethoxyphenvn-N2-⁽3.4.5-trimethoxyphenyn-2.4- pyrimidinediamine from 2-chloro-N-(3,5-dimethoxyphenyl)-4-pyrimidineamine ( 150mg, 0.56mmol) and 3,4,5-trimethoxyaniline (104mg, 0.56mmol) to give the title compound (162mg) as a white solid m.p. 180°. MS m/z 413 (M+H)⁺. The pyrimidineamine starting material was prepared from 3,5-dimethoxy- aniline (257mg, 1.60mmol), 2,4-dichloropyrimidine (250mg, 1.60mmol) and triethylamine (0.25ml, LδOmmol) to give the desired product (237mg) as a white solid m.p. 225°. MS m/z 266 (M+H)+.

EXAMPLE 99

N4-(4-Morpholinophenv0-N2-⁽3.4.5-trimetho^χyphenγl)-2.4-pyrimidine- diamine from 2-chloro-N-(4-morpholinophenyl)-4-pyrimidineamine (300mg, 1.03mmol) and 3,4,5-trimethoxyaniline (189mg, 1.03mmol) to give the title compound (230mg) as a white solid m.p. 214°. MS m/z 438 (M+H)⁺. The pyrimidineamine starting material was prepared from 4-morpholino- aniline (285mg, 1.60mmol), 2,4-dichloropyrimidine (205mg, 1.60mmol) and triethylamine (0.25ml, LδOmmol) to give the desired product (322mg) as a white solid m.p. 221°. MS m/z 291 (M+H)⁺.

EXAMPLE 100

N4-⁽3-Trifluoromethoxyphenyn-N2-⁽3.4.5-trimethoxyphenvn-2.4- pyrimidinediamine from 2-chloro-N-(3-trifluoromethoxyphenyl)-4-pyrimidineamine (230mg, 0.79mmol) and 3,4,5-trimethoxyaniline (145mg, 0.79mmol) to give the title compound (220mg) as a white solid m.p. 154-156°. MS m/z 437 (M+H)+. The pyrimidineamine starting material was prepared from 3-trifluoro- methoxyaniline (354mg, 2. Ommol) 2,4-dichloropyrimidine (218mg, 2.0mmol) and triethylamine (0.3ml, 2.20mmol) to give the desired product (240mg) as a white solid m.p. 161 -163°. MS m/z 290 (M+H)⁺. EXAMPLE 101

N4-(2-Methylbenzimidazol-5-vπ-N2-f3.4.5-trimethoxyphenylamino

2.4-pyrimidinediamine from 2-chloro-N-(2-methylbenzimidazol-5-yl)-4-pyrimidineamine (250mg, 0.96mmol) and 3,4,5-trimethoxyaniiine (176mg, 0.96mmol) to give the title compound (36mg) as a white solid m.p. 179°. MS m/z 407 (M+H)⁺. The pyrimidineamine starting material was prepared from 5-amιno-2- methylbenzimidazole (2147mg, 1 .68mmol), 2,4-dichloropyrimidιne (250mg, 1 .68mmol) and triethylamine (0.25ml, LδOmmol) to give the desired product (344mg) as a pale pink solid m.p. >300°. MS m/z 260 (M+H)⁺.

EXAMPLE 102

N4-(4-(Dimethylamino)phenyl)-N2-(3.4.5-trimethoxyphenylamino)-2.4- pyrimidinediamine from 2-chloro-N-(4-(dimethylamino)phenyl)-4-pyhmidineamine (250mg,

LOmmol) and 2,3,4-trimethoxyaπiline (184mg, LOmmol) to give the title compound (127mg) as a grey solid m.p. 215°. MS m/z 396 (M+H)⁺.

The pyrimidineamine starting material was prepared from N,N-dιmethyl- 1 ,4-phenylenediamine (228mg, 1.6δmmol) 2,4-dichloroprιmιdιne (250mg,

1.68mol) and triethylamine (0.25ml, 1.80mmol) to give the desired product

(402mg) as a white solid m.p. 212°.

EXAMPLE 103 N4-⁽4-⁽Ethoxycarbonylmethynphenvn-N2-f3.4.5-trimethoxyphenyl)- 2.4-pyrimidinediamine from 2-chloro-N-(4-ethoxycarbonylmethyl)phenyl)-4-pyrimidineamine (1.74g, 5.97mmol) and 3,4,5-trimethoxyphenylaniline (1.09g, 5.97mmol) to give the title compound (1.01 g) as a white solid m.p. 143-144°. δ H (CDCI₃) 8.04 (1 H, d, J 5.8Hz), 7.32 (2H, d, J 8.6Hz), 7.26 (2H, d, J δ.6Hz), 7.01 (1 H, br s), 6.66 (2H, s), 6.64 (1 H, br s), 6.15 (1 H, d, J 5.δHz), 4.17 (2H, q, J 7.1 Hz), 3.82 (3H, s), 3.61 (6H, s), 3.60 (2H, s) and 1.27 (3H, t, J 7.1 Hz). MS m/z 439 (M+H)⁺ The pyrimidineamine starting material was prepared from ethyl 4-amino- phenylacetate (1 .79g, 10. Ommol) 2,4-dichloropyrimidine (1 .49g, 10. Ommol) and triethylamine (2.6ml, 20mmol) to give the desired product (2.88g) as a white solid, m.p. 151 -152°.

EXAMPLE 104 N4-t4-Benzyloxyphenyh-N2-(3.4.5-trimethoxy^phenvπ-2.4-pyrimidine- diamine from N-(4-benzyloxyphenyl)-2-chloro-4-pyrimidineamine (25. Og, 80. Ommol) and 3,4,5-trimethoxyaniline (14.7g, 80. Ommol) to give the title compound (18.6g) as a white solid m.p. 161 -164°. MS m/z 459 (M+H)⁺. The pyrimidineamine starting material was prepared from 4- benzyloxyaniiine (20. Og, 0.1 mol), 2,4-dichloropyrimidine (15.0g, 0.1 mol) and triethylamine (15.0ml, 0.11 mol) to give the desired product (30. Og) as a cream solid m.p. 198°. MS m/z 312 (M+H)⁺.

EXAMPLE 105

N4-(3-Ethoxyphenyl)-N2-(3.4.5-triemthoxyphenyn-2.4-pyrimidine- diamine from 2-chloro-N-(3-ethoxyphenyl)-4-pyrimidineamine (125mg, 0.5mmol) and 3,4,5-trimethoxyaniline (92mg, 0.5mmol) to give the title compound (157mg) as a white solid m.p. 79°. MS m/z 397 (M+H)⁺.

The pyrimidineamine starting material was prepared from meta- phenetidine (0.15ml, 1 .68mmol), 2,4-dichloropyrimidine (250mg, 1.68mmol) and triethylamine (0.25ml, LδOmmol) to give the desired product (125mg) as a cream solid m.p. 97°.

EXAMPLE 106

N4-(4-Benzyloxycarbonylmethylphenyl -N2-f3.4.5-trimethoxyphenyl)-

2.4-pyrimidinediamine from N-(4-(benzyloxy-carbonylmethyl)phenyl)-2-chloro-4-pyrimidineamine (3.14g, 8.90mmol) and 3,4,5-trimethoxyaniline (1.63g, 8.90mmol) to give the title compound (3,47g) as a white solid m.p. 141 -143°. δH (CDCI₃) 8.04 (1 H, d, J 5.8Hz), 7.36-7.24 (9H, m), 7.15 (1 H, br s), 6.86 (2H, s), 6.74 (1 H, br s), 6.15 (1 H, d, J 5.8Hz), 5.15 (2H, s), 3.82 (3H, s), 3.81 (6H, s) and 3.66 (2H, s). MS m/z 501 (M+H)⁺. The pyrimidineamine starting material was prepared from benzyl 4-amino- phenylacetate (4.82g, 20. Ommol), 2,4-dichloropyrimidine (2.9δg, 20. Ommol) and triethylamine (5.60ml, 40. Ommol) to give the desired product (3.24g) as a white solid m.p. 149° MS m/z 354 (M+H) +

EXAMPLE 107 N4-<4-Methoxyphenyl)-N2-⁽3.4.5-trimethoxyphenyπ-2.4-pyrimidine- diamine from 2-chloro-N-(4-methoxyphenyl)-4-pyπmιdιneamιne (315mg, 1.30mmol) and 3,4,5-tπmethoxyanιiιne (270mg, 1.50mmol) to give the title compound (470mg) as a white solid m.p.210° MS m/z 363 (M+H)+. The pyrimidineamine starting material was prepared from para-anisidme (616mg, 5 Ommol), 2,4-dιchloropyπmιdιne (745mg, 5. Ommol) and triethyl¬ amine (0 77ml, 5.5mmol) to give the desired compound (450mg) as a white solid m p. 250°.

EXAMPLE 108

N4-ι3-Benzyloxyphenyπ-N2-⁽3.4.5-trimethoxyphenyl)-2.4-pyrimidine- amine from N-(3-benzyloxyphenyl)-2-chloro-4-pyπmιdιneamιne (2.0g, 6 4mmol) and 3,4,5-tπmethoxyanιlιne (1.1 δg, 6 4mmol) to give the title compound (2.60g) as a white solid m.p. 1 δ6° MS m/z 459 (M+H)⁺

The pyrimidineamine starting material was prepared from 3-benzyloxy- anilme (5 Og, 25. Ommol), 2,4-dιchloropyrιmιdιne (3.7g, 25 Ommol) and triethylamine (3.δml, 27.5mmol) to give the desired compound (4.95g) as a white solid m.p. 1 19° MS m/z 312 (M+H)⁺

EXAMPLE 109

N4-π-Phenylsulphonylindol-5-yπ-N2-(3.4.5-trimethoχyphenyn-2.4- pyrimidinediamine from 2-chloro-N-(1 -phenylsulphoπylιndol-5-yl)-4-pyrιmιdιneamιπe (1 09g, 2.70mmol) and 3,4,5-tπmethoxyanilιne (496mg, 2.70mmol) to give the title compound (950mg) as a white solid m.p. 127°. MSm/z 532 (M+H)⁺. The pyrimidineamine starting material was prepared from 5-amιno-1- phenylsulphonylmdole (LOg, 3.9mmol), 2,4-dιchloropyπmιdιne (577mg, 3.9mmol) and triethylamine (0.61 ml) to give the desired product (1 20g) as an orange solid m.p 156° MS m/z 335 (M+H)+. EXAMPLE 110 N4-Cyclohexyl-N2-ι3.4.5-trimethoχyphenvn-2.4-pyrimidinediamine from 2-chloro-N-cyclohexylamino-4-pyrimidineamine (200mg, 0.9mmol) and 3,4,5-trimethoxyaniiine (173mg, O.θmmol) to give the title compound (173mg) as a white solid m.p. 160-161 °. δ H (CDCI3) 7.91 (1 H, d, J 5.9Hz), 6.90 (1 H,s ), 6.67 (2H, s), 5.81 (1 H, d, J 5.9Hz), 4.63 (1 H, br s), 3.86 (6H, s), 3.81 (3H, s), 2.00 (2H, m), 1 .70 (4H, m) and 1 .25 (4H, m). MS m/z 359 (M+H)⁺ The pyrimidineamine starting material was prepared from cyclohexylamme (0.4ml, 3.4mmol), 2,4-dichloropyπmidine (500mg, 3.4mmol) and triethyl¬ amine (0.5ml, 3.7mmol) to give the desired product (270mg) as a white solid m.p. 128°. MS m/z 212 (M+H)⁺.

EXAMPLE 111 N4-(1 -Benzylpiperid-4-yh-N2-_l3.4.5-trimethoxyphenvπ-2.4-pyrimidine- diamine from N-(1 -benzylpiperid-4-yl)-2-chloro-4-pyrιmidineamine (390mg, 1 .3mmol) and 3,4,5-trimethoxyaniline (236mg, 1 .3mmol) to give the title compound (126mg) as a white solid m.p. 147°. δH (CDCI3) 7.90 (1 H, d, _ 5.8Hz), 7.28 (5H, m), 6.94 (1 H, s), 6.86 (2H, s), 5.81 (1 H, d, J 5.8Hz). 4 62 (1 H, br s), 3.65 (6H, s), 3.81 (3H, s), 3.53 (2H, s), 2.83 (2H, m), 2.16 (2H, m), 2.01 (2H, m) and 1 .52 (2H, m).

The pyrimidineamine starting material was prepared from 4-amιno- 1 - benzyipiperidine (0.7ml, 3.4mmol), 2,4-dichloropyrimidine (500mg, 3.4mmol) and triethylamine (0.5ml, 3.7mmol) to give the desired product (650mg) as a white solid m.p. 136°. MS m/z 303 (M+H)+.

EXAMPLE 112 N4-Benzyl-N2-ι3.4.5-trimethoxyphenvn-2.4-pyrimidinediamine from N-benzyl-2-chloro-4-pyrimidineamine (1 .5g, 6.83mmol) and 3,4,5- trimethoxyaniline (1 .31 g, 7.17mmol) to give the title compound (2.30g) as a white solid m.p. 167-168°. MS m/z 367 (M+H)+.

The pyrimidineanine starting material was prepared from benzylamine (3.67ml, 33.56mmol), 2,4-dichloropyrimidine (5.0g, 33.56mmol) and triethylamine (5.14ml, 36.9mmol) to give the desired product (4.21 g) as a white solid, m.p. 135-136°. MS m/z 220 (M+H)+. EXAMPLE 113

N2-l'3.4-Dimethoxy-5-(methylthio)phenyπ-N4-(3-methoxyphenyn-2.4- pyrimidinediamine from 2-chloro-N-(3-methoxy-phenyl)-4-pyrimidineamιne (0.59g, 2.51 mmol) [see Example 92] and 3,4-dimethoxy-5-(methylthio)aniline (0.50g, 2.51 mmol) to give the title compound (0.91 g) as a white solid m.p. 68-70°. δH (CDCI₃) 8.02 (1 H, d, J 5.8Hz), 7.40 (1 H, br, s), 7.26 (1 H, m), 7.08 (1 H, d, J 2.2Hz), 6.90 (4H, m), 6.6δ (1 H, dd, J 8.2, 2.2Hz), 6.20 (1 H, d, J 5.8Hz), 3.82 (3H, s), 3.79 (3H, s), 3.77 (3H, s) and 2.35 (3H, s), MS m/z 399 (M+H)⁺.

The aniline starting material was prepared by treating a solution of 1 ,2- dιmethoxy-3-(methylsulphιnyl)-5-nιtrobenzene ( 1 .64g , 6.69mmol) in methanol (20ml) and concentrated hydrochloric acid (20ml) was treated with anhydrous tin (II) chloride (7.15g, 37.7mmol) and the resulting mixture refluxed for 1 .25h. On cooling to room temperature the mixture was poured into excess 1 M NaOH solution and extracted with CH CI₂. The organic extract was dried (MgSθ ) and evaporated to afford the desired product (1 .33g) as an off-white solid m.p. 106-107°. MS m/z 199 (M+H)⁺.

EXAMPLE 114

N2-(3.4-Dimethoxy-5-methylphenyl)-N4-(3-methoxyphenyπ-2.4- pyrimidinediamine from 2-chloro-N-(3-methoxyphenyl)-4-pyrimidineamine ( 1 .93g) [see Example 92] and 3,4-dimethoxy-5-methylaniline ( 1 .50g) to give the title compound (2.53α) as a pale pink solid m.p. 66-68°. δH (CDCI3) 8.02 (1 H, d, J 5.8Hz), 7.39 (1 H, br s), 7.23 (1 H, t, J 8.1 Hz), 7.08 (1 H, m), 7.05 (1 H, br s), 6.97-6.91 (2H, m), 6.86 (1 H, d, J 2.0Hz), 6.69-6.65 (1 H, m), 6.18 (1 H, d, J 5.8Hz), 3.77 (3H, s), 3.75 (6H, s) and 2.22 (3H, s). MS m/z 367 (M+H)⁺.

The aniline used as starting material was prepared according to the methods of I Sanchezet a/ : Tetrahedron 41, 2355 (1965). EXAMPLE 115

N2-f3.4-DimethoxyDhenvn-N4-(3-methoχyphenγl)-9₁4- pyrimidinediamine from 2-chloro-N-(3-methoxyphenyl)-4-pyrimidineamine (0.77g, 3.27mmol) [see Example 92] and 3,4-dimethoxyaniline (0.50g, 3.27mmol) to give the title compound (0.89g) as a beige solid m.p. 135-138°. δH (CDCI₃) δ.03 (1 H, d, J 5.3Hz), 7.22 (2H, m), 7.13 (1 H, br s), 7.02 (1 H, dd, J 8.6, 2.3Hz), 6.97 (1 H, m), 6.92 (1 H, m), 6.82 (1 H, d, J 87.6Hz), 6.77 (1 H, br s), 6.66 (1 H, dd, J 8.2, 2.3Hz), 6.18 (1 H, d, J 5.δHz), 3.86 (3H, s), 3.83 (3H, s) and 3.77 (3H, s). MS m/z 353 (M+H)⁺.

EXAMPLE 116

N2-₁3-Chloro-4.5-dimethoxyphenvn-N4-(3-methoχyphenyl)-2₁4- pyrimidinediamine hydrochloride from 2-chloro-N-(3-methoxyphenyl)-4-pyrimιdineamine (0.50g, 2.13mmol) [see Example 92] and 3-chloro-4,5-dimethoxyaniline (0.40g, 2.13mmol) to give the title compound (0.55g) as a white soid m.p. 204-205°. δH (d⁶ DMSO) 1 1 .19 (1 H, br s), 10.85 (1 H, br s), 6.00 (1 H, d, J 7.1 Hz), 7.23 (4H, m), 7.12 (1 H, d, J 4.4Hz), 6.75 (1 H, m), 6.59 (1 H, d, J 7.1 Hz), 3.74 (3H, s), 3.69 (3H, s) and 3.65 (3H, s). MS m/z 387 (M+H)+.

The aniline starting material was prepared in a similar manner to the analogous aniline of Example 1 13, from 1 -chloro-2,3-dimethoxy-5- nitrobenzene (1 .08g, 5.δ2mmol), to give the desired product (0.85g) as a white solid m.p. 66-68°. MS m/z 188 (M+H).⁺ The 1 -chloro-2,3-dimethoxy-5-nitrobenzene was prepared by heating a solution of 3-chioro-4,5-dimethoxybenzoic acid (3.50g, 16.2mmol) in glacial acetic acid (15ml) and 70% nitric acid (15ml) at 60° for i h. The reaction was poured onto ice-water and the white precipitate which formed was filtered off, washed with water and dried in vacuo and washed thoroughly with hexane. The hexane washings were evaporated and the residue subjected to column chromatography [silica 20% ethyl acetate- hexane] to give the desired product (1 .03g) as a white solid m.p. 104- 105°. MS m/z 217 (M+H)⁺. The acid used as starting material was prepared according to the method of Y. Qhtani et al. Acta. Chem. Scand., Ser B. B36, 613 (1982). EXAMPLE 117

N2-(3-Benzyloxy-4.5-dimethoxyphenγlVN4-(3-methoχyphenγl)-2.4- pyrimidinediamine from 2-chloro-N-(3-methoxyphenyl)-4-pyπmιdιneamιne (162mg, 0.69mmol) [see Example 92] and 3-benzyloxy-4,5-dιmethoxyanιlιne (180mg, 0.69mmol) to afford the title compound (255mg) as an off-white solid m.p. 83-84°. δH (CDCI₃) 8.01 (1 H, d, J 5.8Hz), 7.45-7.22 (6H, m), 6.95 (3H, m), 6.90 (1 H, d, J 7.6Hz), 6.79 (1 H, d, J 2.4Hz), 6.66 (1 H, d, J 7.6Hz), 6.4δ (1 H, s), 6.17 (1 H, d, J 5.δHz), 5.1 1 (2H, s), 3.86 (3H, s), 3.82 (3H, s) and 3.78 (3H, s). MS m/z 369 (M+H)⁺.

The 3-benzyloxy-4,5-dιmethoxyanιlιne was prepared by heating a solution of 1 -benzyloxy-2,3-dιmethoxy-5-nιtrobenzene (1 .80g, 6.23mmol) in ethanol (15ml) with saturated aqueous sodium hydrosulphite (20ml) at reflux for 2h. An additional quantity of sodium hydrosulphite (20ml) was added and reflux continued for a further 4h. The reaction mixture was reduced to a small volume then diluted with water and extracted three times with ethyl acetate. The organic phase was dried (MgSθ₄) and evaporated to give the crude product which was recrystallised from ether- hexane to give the desired product (356mg) as a white crystalline solid m.p. 118-120°. MS m/z 260 (M+H)⁺.

The 1 -benzyloxy-2,3-dιmethoxy-5-nιtrobenzene used as starting material was prepared by treating a solution of 1 -benzoyl-2,3-dιmethoxybenzene (7.25g, 29.66mmol) in glacial acetic acid (15ml) portionwise with 70% nitric acid (2.84ml) at room temperature. After 2h, the reaction mixture was poured into ice-water and extracted with ethyl acetate. The organic phase was washed with 1 M NaOH, then brine, dried (MgSθ₄) and evaporated. The crude product was purified by column chromatography [silica 20% ethyl acetate-hexane] to give, after recrystallisation from CH₂CI₂-hexane, the desired product (1.89g) as a white solid m.p. 101 -102°. MS m/z (relative intensity) 239 [(M+H)⁺, 2%] 91 [100%].

1 -Benzyloxy-2,3-dimethoxybenzene was prepared by the method of F Dietl et al Synthesis, 626 (1965). EXAMPLE 118

N2-t3-Acetamido-4.5-dimethoχy^phenvh-N4-(3-mβthoxyphenyn2₁4- pyrimidinediamine from 2-chloro-N-(3-methoxyphenyl)-4-pyrιmidιneamine (0.81 g, 3.43mmol) [see Example 92] and 3-acetamιdo-4,5-dιmethoxyaniline (0.70g, 3.43mmol) to give the title compound (573mg) as an off-white solid m.p. 195-197°. δH (d6 DMSO) 9.26 (1 H, s) 9.10 (1 H, s), 9.01 (1 H, s), 7.97 (1 H, d, J 5.7Hz), 7.74 (1 H, s), 7.46-7.25 (3H, m), 7.15 (1 H, t, J 8 1 Hz), 6.54 (1 H, d, J 8.1 Hz), 6.18 (1 H, d, J 5.7Hz), 3.67 (9H, m) and 2.06 (3H, s) MS m/z 410 (M+H)L

The 3-acetamιdo-4,5-dιmethoxyanιlιne was prepared in an analogous manner to the compound of Example 38, from 1 -acetamιdo-2,3- dιmethoxy-5-nιtrobenzene (1.65g, 6.δδmmol) to afford the desired product (1.37g) as a brown solid m.p. 156-160° MS m/z 211 (M+H)⁺. The 1 -acetamιdo-2,3-dιmethoxy-5-nιtrobenzene was prepared in an analogous manner to the nitrobenzene of Example 117 from 1 -acetamιdo- 2,3-dιmethoxybenzene (1.91 g, 9.6mmol) to give the desired product (1.70g) as a white solid m.p. 63°. MS m/z 241 (M+H)+. The 1 -acetamιdo-2,3-dιmethoxybenzene was prepared by treating a solution of 2,3-dιmethoxyanιlιne (1.70g, 1 1.1 mmol) in carbon tetrachloride (25ml) with methanol (0.2ml) followed by acetic anhydride (3.67g, 36. Ommol) and heating the resulting mixture at reflux for 1 h The cooled reaction mixture was diluted with CH₂CI₂, washed with water, dried (MgSθ4) and evaporated to give the desired product (2.1 1 g) as a beige solid m.p. 164-165°. MSm/z 196 (M+H)⁺.

EXAMPLE 119 5-Bromo-N2.N4-Bis⁽trimethoxyphenyπ-2.4-^pyrimidinediamine from 5-bromo-2-chloro-N-(3,4,5-trιmethoxyphenyl)-4-pyrιmιdιneamιne (936mg, 2.5mmol) and 3,4,5-tπmethoxyaniiιne (459mg, 2.5mmol) to give the title compound (160mg) as a white solid m.p. 166-191°. δH (CDCI3) 8.16 (1 H, s), 7.45 (1 H, br s), 6.98 (1 H, br s), 6.77 (2H, s), 6.74 (2H, s), 3.83 (3H, s), 3.80 (3H, s), 3.71 (6H, s), 3.66 (6H, s), MS m/z 523 [(M+H)+, 8 Br,100%] 521 [(M+H)⁺, 9Br, 100%]. The pyrimidineamine starting material was prepared from 5-bromo-2,4- dichloropyπmidine (1.14g, 5. Ommol), 3,4,5-trιmethoxyanιlιne (916mg, 5. Ommol) and triethylamine (0.77ml, 5.5mmol) following the method used for the pyrimidineamine starting material in Example 89. This gave the desired compound as a white solid (1 .71 g) m.p. 185-166°. MS m/z 378 [(M+H)⁺, 22%], 376 [(M+H)⁺, 100%], 374 [(M+H)+, 78%].

EXAMPLE 120

N4- 4-Hvdroxyphenvπ-N2-l'3.4.5-trimethoxyphenvn-2.4-pyrimidine- diamine

In a manner analogous to the preparation of the compound of Example 49 from the compound of Example 104 (3.0g, 6.55mmol), ammonium formate (1 .20g, 19.60mmol) and 10% palladium on carbon (300mg) to give the title compound (2.40q) as a white solid m.p. 1 12-1 15°. δH (CDCI3) 7/96 )1 H. d, J 5.9Hz), 7.16 (2H, d, J_8.7Hz), 7.06 (1 H, s), 6.83 (1 H, s), 6.80 (3H, m), 6.68 (1 H, s), 6.01 (1 H, d, J 5.9Hz), 3.79 (3H, s) and 3.78 (6H, s). MS m/z 369 (M+H)⁺.

EXAMPLE 121

N4-ι4-(3-Aminopropoxy)phenv0-N2-^('3.4.5-trimethoxyphenγl)-2.4- pyrimidinediamine dihydrochloride In a manner analogous to the preparation of the compound of Example 48 from N4-(4-(3-phthalιmιdopropoxy)phenyl)-N2-(3,4,5-tπmethoxyphenyl)- 2,4-pyπmιdιnedιamιne (400mg, 0.72mmol) and hydrazine monohydrate (0.1 ml, 2.16mmol) to give the title compound (108mg) as a buff solid m.p. 258°. δH (d6 DMSO) 1 1 .04 (1 H, s), 10.54 (1 H, s), 8.06 (3H, s), 7.91 (1 H, d, J 7.3Hz), 7.55 (2H, m), 6.86 (2H, d, J 6.7Hz), 6.78 (2H, s), 6.49 (1 H, s), 4.03 (2H, m), 3.65 (9H, s), 2.94 (2H, m) and 2.02 (2H, m). MS m/z 426 (M+H)⁺.

The pyπmidinediemine starting material was prepared according to the method of Example 45 from the compound of Example 120 and 3- bromopropylphthalim.de EXAMPLE 122

N4-ι4-(Carboxymethvnphenyn-N2-(3.4.5-trimethoxyphenvn-2.4- pyrimidinediamine The compound of Example 1 03 (250mg, 0.57mmol) in a solution of ethanol (10ml) containing 6N aqueous NaOH (2.0ml) was heated a reflux for 2h. On cooling the ethanol was removed under reduced pressure and the basic solution brought to pH 5 with 2M hydrochloπc acid. The resulting solution was concentrated under reduced pressure, and the residue taken up in hot ethanol (20mi). This solution was filtered and the filtrate evaporated under reduced pressure to give a residue which was crystalised from ethanol to give the title compound (89mg) as a white solid m.p. 144-150°. MS m/z 411 (M+H)+.

EXAMPLE 123 N4-⁽4-⁽ferf-Butoxycarbonylmethoxy⁾phenvh-N2-ι3.4.5-trimethoxy- phenyl)-2.4-pyrimidinediamine

In a manner analogous to the preparation of the compound of Example 50, from the compound of Example 120 (500mg, 1 40mmol), rert-butyl bromoacetate (0.2ml, 1 40mmol) and NaH (60% dispersion in oil) (60ml, 1.50mmol) to give the title compound (380mg) as a green solid m p 139° MS m/z 4δ3 (M+H)+

EXAMPLE 124 N4-(4-(2-Hvdroxyethvn^phenvn-N2-f3.4.5-trimethoχyphenvn-2.4- pyrimidinediamine

In a manner analogous to the preparation of the compound of Example 62 (267mg, 0.5mmol) from the compound of Example 106 and L1AIH₄ (40mg, 1 1 mmol) to give the title compound (10δmg) as a light pink solid m p 172°. MS m/z 397 (M+H)⁺.

EXAMPLE 125

N4-f4-<^,Carboxymethoxy)phenyl⁾-N2-t^'3.4.5-trimethoxyphenvπ-2.4- pyrimidinediamine

A solution of the compound of Example 123 (300mg, 0.62mmol) in CH CI₂ (20ml) was treated with trifluoroacetic acid (3.0ml) and stirred at room temperature for 5h After this time the reaction was concentrated under reduced pressure to give a brown oil, which was subjected to column chromatography [silica CH₂CI /methanol/acetιc acid/water 86.5:10:2.1.5] to give the title compound (123mg) as a white solid m.p. 159° after recrystallisation from methanol. MS m/z 427 (M+H)⁺ EXAMPLE 126 4-Phenoxy-N2-.'3.4.5-trimethoxyphenyn-2-pγrimidineamine

2-Methylsulphonyl-4-phenoxypyrιmιdine (0.76g, 3.04mmol) and 3,4,5- trimethoxyaniiine (0.56g, 4. Ommol) were heated as a melt at 140° for 2h. On cooling the residue was partitioned between ethyl acetate (50ml) and 2M hydrochloric acid (50ml), and the organic Iayer was washed with water (50ml), dried (MgS04) and concentrated under reduced pressure. The residue was subjected to column chromatography [silica 20% ethyl acetate-hexane] to give the title compound (18mg) as a white solid m.p. 149° after recrystallisation from ethyl acetate. δH (CDCI₃) 6.26 (1 H, d, J 5.6Hz), 7.39 (2H, m), 7.26 (1 H,m), 7.16-7.15 (2H, m), 6.73 (2H, s), 6.31 (1 H, d, J 5.6Hz), 3.77 (3H, s) and 3.64 (6H, s) MS m/z 354 (M+H)⁺. The pyrimidine used as starting material was prepared by the addition of 50% 3-chloroperoxybenzoιc acid (15.19g, βδ. Ommol) to a solution of 4- phenoxy-2-thιomethylpyrιmιdιne (5.0g, 22.9mmol) in CH CI₂ (100ml) at 0°. The resulting mixture was allowed to rise to room temperature over 12h, and was then washed with 2M aqueous NaOH (2x100ml), saturated aqueous sodium sulphite (2x100ml), dried (MgSθ4) and concentrated under reduced pressure. The residue was subjected to column chromatography to give the desired product (0.61 g) as a colourless oil δH (CDCI3) 8.49 (1 H, d, J 5.5Hz), 7.45 (2H, t, J 8.0Hz), 7.33-7.26 (1 H, m), 7.19-7.16 (2H, m), 6.85 (1 H, d, J 5.5Hz) and 3.23 (3H, s). The 4-phenoxy-2-thιomethyipyrιmιdιne used as starting material was prepared by heating a solution of 4-fluoro-2-thιomethylpyπdιne [N. Pie et a/., J. Het. Chem. 31, 131 1-1315 (1994)] (9.98g, 69.3mmol) and phenol (6.58g, 70. Ommol) in dry DMF (100ml) in the presence of caesium carbonate (22. δg, 70. Ommol) at 40° for 6h. The reaction was concentrated in vacuo and the residue was partitioned between ethyl acetate (100ml) and water (100ml). The organic Iayer was dried (MgSθ4), concentrated under reduced pressure and the residue subjected to column chromatography [silica 40% ethyl acetate-hexane] to give the desired product (7.01 g) as a colourless oil. δH (CDCI3) 8.33 (1 H, dd, J 4.7, 0.9Hz), 7.40 (2H, dd, 8.0, 1 .1 Hz), 7.26 (1 H, m), 7.23 (2H, m), 6.46 (1 H, dd, J 4.7, 0.9Hz) and 2.37 (3H, s). MS m/z 219 (M+H)L EXAMPLE 127 N.N'-Bisι3.4.5-Trimethoxy^phenvn-2.4-^pyrimidinediamine

A solution of 2,4-dichloropyrimidine ( LOg, 6.7mmol) , 3,4,5- trimethoxyaniline (2.5g, 13.4mmol) and triethylamnine (1.8ml, 12.6mmol) was heated at reflux for 5h. On cooling the resulting precipitate was collected and recrystallised from ethyl acetate to give the title compound (450mg) as a purple solid m.p. 180°. MS m/z 443 (M+H)+.

EXAMPLE 128 4-Benzoyl-N-⁽3.4.5-trimetho^χy^phenvn-2-pγrimidineamine

To a solution of N-methoxy-N-methyl-2-(3,4,5-trimethoxyphenylamino)- pyrimidine-4-carboxamide (0.82g, 2.30mmol) in THF (40ml) cooled to -7δ° under a nitrogen atmosphere was added phenylmagπesium bromide [Aldrich 1 M/THF] (6.90ml, 6.90mmol) dropwise. On completion of addition the reaction was left to warm to room temperature over 3h and aqueous NH₄CI solution (10ml) was then added and the mixture concentrated under reduced pressure. The residue was partitioned between water (20ml) and ethyl acetate (40ml), the organic Iayer dried (MgS0 ) and evaporated. The residue was subjected to column chromatography [silica 50% hexane-ethyl acetate] and after recrystallisation from ethyl acetate- hexane the title compound (123mg) was obtained as a yellow solid m.p 135°. δH (CDCI3) 6.65 (1 H, d, J 4.8Hz), 8.05 (2H, dd, J 5.1 , 2.0Hz), 7.61 (1 H, dd, J 5.3, 2.0Hz), 7.46 (2H, m), 7.24 (1 H, d, J 4.8Hz), 3.79 (3H, s) and 3.63 (6H, s). MS m/z 366 (M+H)L The pyrimidine-4-carboxamide used as starting material was prepared by treating a solution of 4-carboxy-N-(3,4,5-trimethoxyphenyl)-2-pyrimidine- amine hydrochloride (2.0g, 5.86mmol) and triethylamine (2.0ml, 14.0mmol) in CH₂CI₂ (40ml), cooled to -20° under a nitrogen atmosphere, with isobutylchloroformate (1.1 ml, 7.0mmol). The resulting mixture was stirred for 20min. before N.O-dimethylhydroxylamine hydrochloride (683mg, 7.0mmol) and triethylamine (1.0ml, 7.0mmol) were added and the reaction allowed to warm to room temperature over 3h. After washing with 2M hydrochloric acid (1 x50ml) and 2M NaOH solution (1 x50ml), the reaction was dried (MgS04) and concentrated under reduced pressure. The residue was subjected to column chromatography [silica ethyl acetate] to give the desired product (1.02g), as a yellow oil. δH (CDCI3) 8.51 (1 H, d, J 4.9Hz), 7.21 (1 H, br s), 6.92 (3H, br s), 3.86 (6H, s), 3.82 (3H, s), 3.69 (3H, br s) and 3.36 (3H, s). MS m/z 349 (M+H)+

The 4-carboxy-N-(3,4,5-trιmethoxyphenyl)-2-pyrιmιdιneamιne hydro¬ chloride was prepared by heating a solution of 4-cyano-N-(3,4,5- tπmethoxyphenyl)-N-pyπmιdιneamιne (2.86g, 10 49mmol) in aqueous 2N NaOH (60ml) and ethanol (10ml) at reflux for 3h. On cooling the reaction mixtrure was adjusted to pH 3 with 2M hydrochloric acid, and the resulting precipitate collected and dried to give the desired product (2.7g) as an orange solid m.p. 241 °. MS m/z 306 (M+H)⁺. The 4-cyano-N-(3,4,5-tπmethoxyphenyl)-2-pyπmιdιneamιne was prepared by heating a solution of 2-chloro-4-cyanopyπmιdιne [G. Davies et al. J. Het. Chem. 1, 130-133, (1963)] (1 1 . Og, 76.6mmol), 3,4,5-tπmethoxy aniline (14 4g, 79.4mmol) and triethylamine (12.0ml) in ethanol at reflux for 4h. On cooling the resulting precipitate was collected and dried to give the desired product (1 3.70 g) as a yellow solid m p. 1 65° MS m/z 287 (M+H)⁺.

EXAMPLE 129 N-Phenyl-2-f3.4.5-trimethoχyphenylamino^pyrimidine-4-carboxamide In a manner analogous to the preparation of the pyπmιdιne-4-carbox amide intermediate of Example 1 28, from 4-carboxy-N-(3,4,5- tπmethoxyphenyl)-2-pyrιmιdιneamιne hydrochloride ( LOg, 2.92mmol), isobutylchloroformate (0.4ml, 3 07mmol), aniline (0.28ml, 3 07mmol) and triethylamine (1 .22ml, 8.76mmol) to give the title compound (655mg) as a yellow solid m.p 1 58-159°. δH (CDCI₃) 9.66 (1 H, br s), 8.67 (1 H, d, J 4.9Hz), 7.71 -7.67 (2H, m), 7.58 (1 H, d, J 4.9Hz), 7.40-7.34 (3H, m), 7.19- 7.14 (1 H, m), 6.8δ (2H, s), 3.87 (6H, s) and 3.86 (3H, s). MS m/z 381 (M+H)⁺.

EXAMPLE 130

4-Phenylsulphonamido-N2-t3.4.5-trimethoxyphenyl⁾-2-pyrimidine- amine

A solution of 2-chloro-4-phenylsulphonamιdopyrιmιdιne (250mg ,

0.93mmol) and 3,4,5-trιmethoxyanilιne ( 1 87mg , 1 02mmol) in ethoxyethanol (5ml) was heated at 140° for 12h. After this time the solvent was evaporated under reduced pressure and the residue partitioned between ethyl acetate (10ml) and water (10ml). The organic Iayer was washed with water (2x25ml), dried (MgS0₄) and evaporated and the resulting solid subjected to column chromatography [silica-ethyl acetate] to give the title com^pound (187mg) as a buff solid, m.p. 222-223°. δH (CDCI₃) 8.11 (1 H, d, J 5.9Hz), 7.92 (3H, m), 7.57 (1 H, m), 7.50 (3H, m), 6.80 (2H, s), 6.66 (1 H, d, J 5.9Hz), 3.81 (6H, s) and 3.21 (3H, s). MS m/z 417 (M+H)+.

The 2-chloro-4-phenylsulphonamidopyrιmidine was prepared by heating 2,4-dichloropyrimidine (1.42g, 9.5mmol), benzenesulphonamide (4.5g, 29. Ommol) and potassium, carbonate (3.3g, 24.0mmol) in DMA (25ml) for 0.75h). The reaction was cooled to 5°, water (20ml) added, and ad_justed to pH2.5 with 2M HCl. The resulting precipitate was collected to give the desired product (1.8g) as a light yellow solid m.p. 164° MS m/z 270 (M+H)⁺.

EXAMPLE 131

N4-⁽.-erf-Butoxycarbonyπ-N2-t^'3.4.5-trimethoxyphenyπ-2.4-pyrimidine- diamine

To a solution of diphenylphosphorylazide (0.92ml, 4.3mmol) in tert-butyl alcohol (10ml), was added triethylamine (1 ,2ml, 8.5mmol) and 4-carboxy- N-(3,4,5-trimethoxyphenyl)-2-pyrιmidιneamιne (LOg, 2.9mmoi), [see Example 121] and the mixture heated at reflux for 2h. After this time the solvent was evaporated and the residue partitioned between ethyl acetate (100ml) and water (100ml). The organic Iayer was washed with water (100ml), dried (MgSθ4) and concentrated under reduced pressure. Column chromatography [silica 2% methanol CH₂CI₂] gave the title compound (850mg) as a light yellow solid m.p. 124°. δH (CDCI3) δ.27 (1 H, d, J 5.8Hz), 7.45 (1 H, br s), 7.32 (1 H, d, J 5.8Hz), 7.20 (1 H, br s), 6.81 (2H, s), 3.85 (6H, s), 3.81 (3H, s) and 1.53 (9H, s). MS m/z 377 (M+H)+.

EXAMPLE 132

4-Phenylcarboxamido-N-f3.4.5-trimethoxyphenvh-2-pyrimidineamine In a manner analogous to the proparation of the pyrimidine-4-caboxamιde intermediate of Example 128, from benzoic acid (66mg, 0.54mmol), isobutylchloroformate (0.078ml, 0.6mmol) N2-(3,4,5-trimethoxyphenyl)- 2,4-pyrimidιnediamine (150mg, 0.54mmol) to give the title compound (30mg) as a white solid m.p. 88-89°. δH (CDCI₃) β.39 (1 H, d, J 5.6Hz), 8.32 (1 H, br s), 7.89-7.85 (2H, m), 7.74 (1 H, d, J 5.6Hz), 7.64-6.50 (3H,m), 6.99 (1 H, br s), 6.85 (2H, s), 3.88 (6H, s) and 3.84 (3H, s). MS m/z 381 (M+H)⁺. The N2-(3,4,5-trimethoxyphenyl)-2,4-pyrimidinediamine used as starting material was prepared by treating a solution of the compound of Example 131 (600mg, 1 .6mmol) in methanol (10ml) with 2M hydrochloric acid (10ml), which was then heated at reflux for 2h. On cooling the methanol was evaporated and the aqueous solution adjusted to pH 12 with 2N aqueous NaOH. The basic solution was extracted with CH2CI2 (3x30ml), then the combined organic extracts were washed with saturated brine (1 x20ml), dried (MgS04) and concentrated under reduced pressure to give the desired product (340mg) as a light yelllow solid m.p. 89°. MS m/z 277 (M+H)⁺.

EXAMPLE 133

N2-(3.4-Dimethoxy-5-hydroxy -N4-f3-methoxyphenvh-2.4-pyrimidine- diamine

In a manner analogous to the preparation of the compound of Example 49, from the compound of Example 1 17 (200mg, 0.44mmol) to give the title compound (109mq) as a white solid m.p. 94-97°. δH (d⁶ DMSO) 9.24 (1 H, s), 8.86 (1 H, s), 8.84 (1 H, s), 7.97 (1 H, d, J 6.0Hz), 7.32 (1 H, d, J 9.2Hz), 7.26 (1 H, s), 7.17 (1 H, t, J 8.1 Hz), 6.89 (2H, m), 6.54 (1 H, d, J 8.3Hz), 6.17 (1 H, d, J 5.7Hz), 5.73 (3H, s), 5.69 (3H, s) and 3.63 (3H, s).

The compound of Examples 134-137 were prepared in a similar manner to the compound of Example 1 from the starting materials shown: EXAMPLE 134 5-Methoxy-4-phenyl-N-(3.4.5-trimethoχyphenvπ-2-pyrimidineamine from 3,4,5-trimethoxyphenylguanidine nitrate (1 .43g, 5. Ommol), 3- dimethylamino-2-methoxy-1 -phenyl-2-propen-1 -one (1 .03g, 5. Ommol) and NaOH (220mg) to give the title compound (200mg) as straw coloured crystals m.p. 166-170°. δH (d<5 DMSO) 9.36 (1 H, br s), 8.47 (1 H, s), 8.09 (2H, m), 7.48 (3H, s), 7.26 (2H, s), 3.88 (3H, s), 3.75 (6H, s) and 3.59 (3H, s). MS m/z 368 (M+H)⁺. The 3-dimethylamino-2-methoxy-1 -phenyl-2-propen-1 -one was prepared in a similar manner to the analogous starting material of Example 61 , from 2-methoxyacetophenone (4.51 g, 30. Ommol) to give the desired product (5.30g) as an orange oil. MS m/z 206 (M+H)⁺.

EXAMPLE 135

4-Phenyl-5-thiomethyl-N-f3.4.5-trimethoxyphenyπ-2-pyrimidineamine from 3,4,5-trimethoxyphenylguanidine nitrate ( 1 .43g, 5. Ommol), 3- dimethylamino-1 -phenyl-2-thiomethyl-2-propen-1 -one (1 .1 1 g, 5. Ommol) and NaOH (220mg, 5.5mmol) to give the title compound (79.8mg) as light yellow crystals m.p. 143°. δH (CDCI₃) 8.52 (1 H, s), 7.88-7.85 (2H, m), 7.49 (3H, m), 7.16 (1 H, br s), 7.02 (2H, s), 3.86 (6H, s), 3.82 (3H, s) and 2.26 (3H, s). MS m/z 384 (M+H)+. The 3-dimethylamino-1 -phenyl-2-thiomethyl-2-propen-1 -one was prepared in a similar manner to the analogous starting material of Example 61 , from 2-thiomethylacetophenone (4.28g, 25.7mmol) to give the desired product (3.41 g) as an orange oil. δH (CDCI3) 7.49-7.47 (2H, m), 7.46 (1 H, s) , 7.41 -7.34 (3H, s), 3.27 (6H, s) and 2.12 (3H, s) MS m/z 222 (M+H)+.

EXAMPLE 136

5-Nitro-4-phenyl-N-ι3.4.5-trimethoxyphenvπ-2-pyrimidineamine from 3,4,5-trimethoxyphenylguanidine nitrate (1 .14g, 4. Ommol) , 3- dimethylamino-2-nitro-1 -phenyl-2-propen-1 -one (0.δ8g, 4. Ommol) and NaOH (176mg, 4.4mmol) to give the title compound (1 .33g) as an orange powder m.p. 186-169°. δH (d^ DMSO) 10.60 (1 H, br s), 9.16 (1 H, s), 7.61 (2H, m), 7.56-7.46 (3H, m), 7.25 (2H, br s), 3.74 (6H, s) and 3.63 (3H, s). MS m/z 383 (M+H)⁺.

The 3-dimethylamino-2-nitro-1 -phenyl-2-propen-1 -one was prepared in a similar manner to the analogous starting material of Example 61 from benzoylnitromethane (4.12g, 25. Ommol) to give the desired product (1 .27g) as yellow crystals m.p. 103-105°. MS m/z 243 [(M+Na)⁺, 70%] 221 [(M+H)+, 100%]. EXAMPLE 137

5-Ethoxycarbonyl-4-phenyl-N-(^'3.4.5-trimethoxypheny0-2-pyrimidine- amine from 3,4,5-tπmethoxyphenylguanιdιne nitrate (2.86g, 1 0. Ommol), 3- dιmethylamιno-2-ethoxycarbonyl- 1 -phenyl-2-propen- 1 -one (2.47g, 10. Ommol) and NaOH (440mg, 1 LOmmol) to give the title compound (3.10g) as straw coloured crystals m.p 160-161 °. δH (d⁶ DMSO) 10.12 (1 H, br s), 8.67 (1 H, s), 7.59 (2H, m), 7.50-7 45 (3H, m), 7.28 (2H, s), 4 1 1 (2H, q J 7.1 Hz), 3.74 (6H, s), 3.61 (3H, s) and 1 06 (3H, t, J 7 1 Hz). The 3-dιmethylamιno-2-ethoxycarbonyl-1 -phenyl-2-propen-1 -oπe was prepared in a similar manner to the analogous starting material of Example 61 , from ethylbenzoylacetate (5 77g, 30. Ommol) to give the desired product (6.01 g) as a yellow solid m p. 64-65° MS m/z 248 (M+H)⁺

EXAMPLE 138

5-Amino-4-phenyl-N-ι3.4.5-trimethoχyphenv0-2-pyrimidineamine In a manner analogous to the preparation of the compound of Example 38, from the compound of Example 136 (500mg, 1 32mmol) to give the title compound (260mg) as a yellow solid m.p 149-150°. δH (CDCI3) 8.09 (1 H, s), 7.87-7.64 (2H, m), 7.51 -7.47 (3H, m), 7 00 (2H, s), 6.87 (1 H, br s), 3.86 (6H, s), 3.81 (3H, s) and 3.52 (2H, br s) MS m/z 353 (M+H)+

EXAMPLE 139 4-Phenylsulphanyl-N-(3.4.5-trimethoxyphenvn-2-pyrimidineamine

In a manner analogous to the preparation of the compound of Example 89, from 2-chloro-4-phenyl-sulphanylpyπmιdιne (1 .41 g, 6.35mmol) and 3,4,5- tπmethoxyphenylaniline (1 .16g, 6.35mmol) to give the title compound (1.43g) as an off-white solid m.p. 1 10°. δH (CDCI3) 8.04 (1 H, d, J 5 4Hz), 7.59 (2H,m), 7.47 (3H, m), 7.22 (1 H, br s), 6.88 (2H, s), 6.18 (1 H, d, J 5.4Hz), 3.83 (6H, s) and 3.81 (3H, s). MS m/z 370 (M+H)+. The 2-chloro-4-phenylsulphanylpyπmιdιne was prepared by treating a solution of thiophenol (4.7ml, 45.4mmoi) in THF (30ml) at 0°, with a 1 .0M solution of sodium bιs(tπmethylsilyl)amιde in THF (45.4ml) and the mixture stirred for 0.5h After a thick white precipitate had formed, 2,4- dichloropyπmidine (6.83g, 45.4mmol) and DMA (70ml) were added and the mixture heated at 120° for 2h. The reaction was then concentrated under reduced pressure and the residue partitioned between wat er and ethyl acetate. The aqueous Iayer was further extracted with ethyl acetate and the combined organic layers were dried (MgS0₄) and evaporated. The residue was subjected to column chromatography (silica 20% ethyl acetate-hexane) to give the desired product (2.66g) as a white solid δH 8.17 (1 H, d, J 5.5Hz), 7.59 (2H, m), 7.53 (3H, m) and 6.62 (1 H, d, J 5.5Hz). MS m/z 223 (M+H)+.

EXAMPLE 140

N-(4-(2-(3.4.5-Trimethoxyphenylamino)pyrimidin-4-vn-2-amin^Q- acetamide

A solution of N-(4-(2-(3,4,5-trιmethoxyphenylamιno)pyπmιdιn-4-yl)phenyl)- 2-N-(9-fluorenylmethoxycarbonyl)amιnoacetamιde (210mg, 0 33mmol) and piperidine (0.5ml, 5mmol) in DMF (2.5ml) was stirred at room temperature for 0.5h. The sovlent was evaporated and the residue triturated with hot ethyl acetate to give the title compound (25mg) as an off-white solid m.p. 213°. δH (d⁶ DMSO) 9.47 (1 H, s), 8 49 (1 H, d, J 5.4Hz), 8.1 1 (2H, d, J 8.3Hz), 7.75 (2H, d, J 8 2Hz), 7.29 (3H,m), 3 80 (6H, s) and 3.61 (3H, s). MS m/z 410 (M+H)⁺.

The starting material for the above process was obtained by treating a solution of the compound of Example 39 (250mg, 0.71 mmol) in CH₂CI₂ (10ml) with N-FMOCglycyl chloride (337mg, 1 07mmol) and 5% aqueous Na₂C0₃ solution (8ml). After the mixture had been stirred for 1 h at room temperature, the aqueous phase was discarded and the organic Iayer dried (MgS04) and evaporated. The residue was subjected to column chromatography [silica 10% methanol-CH₂CI₂] to give the desired product (235mg) as an off-white solid, m.p. 102° (decomp.). δH (d⁶ DMSO) 10.23 (1 H, s), 9.48 (1 H, s), 8.4δ (1 H, d, J 5.1 Hz), 8.15 (2H, d, J 8.3Hz), 7.68 (2H, d, J 7.3Hz), 7.73 (4H, m), 7.64 (1 H, m), 7.43-7.30 (7H, m), 4.34- 4.20 (3H, m), 3.79 (8H, m) and 3.62 (3H, s).

EXAMPLE 141

6-Methyl-4-phenoxy-N- 3.4.5-trimethoxyphenyl)pyri idine-2-aminP 4-Chloro-6-methyl-N-(3,4,5-tπmethoxyphenyl)pyrιmιdιne-2-amιne (0 30g, 0.97mmol) was added to a solution of sodium phenoxide (0.97mmol) [prepared from phenol (0.091 g, 0.97mmol) and NaH (60% in mineral oil; 0.039g, 0.97mmol)], and heated to 80° for 17h with stirring. Water was added and the mixture was extracted with CH₂CH (2x30ml), the organic phases were dried (MgSθ4) and evaporated to a yellow oil. The residue was subjected to chromatography (silica, 2% methanol/CH₂CH₂ ) to give the title compound (0.046g). A portion was recrystallised from methanol to give an off white crystalline solid m.p. 175°. δ\-\ (CDCI₃) 2.38 (3H, s), 3.64 (6H, s), 3.77 (3H, s), 6.19 (1 H, s), 6.78 (2H, s), 6.95 (1 H, s), 7.15 (2H, d, J 7.5Hz), 7.23 (1 H, m) and 7.39 (2H, t, J 7.5Hz).

The am e starting material for this reaction was prepared from the following: 4-Chloro-6-methyl-N-ι3.4.5-trimethoxyphenyhpyrimidine-2-amine (b)

6-Methyl-2-(3,4,5-tπmethoxyphenylamιno)-4(3H)pyrιmιdιnone ( LOg , 3.63mmol) was dissolved in phosphoryl chloride and heated to 100° for 2 h The mixture was cooled to room temperature and evaporated in vacuo to a viscous oil The oil was dissolved in CH2CH2 (50ml) and washed twice with saturated aqueous NaHCθ3, the organic phases dried (MgSθ4) and concentrated in vacuo The residue was subjected to column chromatography (silica, 2% methanol/CH CH2) to afford the title c o m p o u n d as an off white solid ( 1 .03g) A small amount was recrystallised from ethyl acetate to give fine off-white needles m.p 1 95- 196° δ_H (CDCI3) 2.40 (3H, s), 3.82 (3H, s), 3.68 (6H, s), 6.64 (1 H, s), 6.95 (2H, s), and 7.07 (1 H, br s).

The pyπmidinone starting material for this reaction was prepared as follows:

6-Methyl-2-(3.4.5-trimethoxyphenylamino)-4-(3H)pyrimidinone

To a solution of sodium methoxide (0.96g, 1 .79mmol) in methanol was added ethyl-3-oxo-butanoate (0.23g, 1 .79mmol) and 3,4,5-trιmethoxy- phenylguanidinium nitrate (0.5g, 1 .79mmol). The mixture was stirred at reflux for 17h The reaction mixture was cooled to room temperature and evaporated in vacuo to give a dark grey solid. Recrystallisation from ethanol gave the title compound (0.25g, 49%) as a light grey solid, m.p. 228-230° (dec) δ_H (d^DMSO) 2.12 (3H, s), 3.61 (3H, s), 3.74 (6H, s), 5.69 (1 H, s), 7.02 (2H, s), 8.70 (1 H, br s) and 10 47 (1 H, br s). BIOLOGICAL ACTIVITY

The following assays were used to demonstrate the activity and selectivity of compounds according to the invention:

p56l£k kinase assay

The tyrosine kinase activity of p56^lck was determined using a RR-src peptide (RRLIEDNEYTARG) and [γ-33p]ATP as substrates. Quantitation of the ³³P-phosphorylated peptide formed by the action of p56^lck was achieved using an adaption of the method of Geissler et al (J. Biol. Chem.

(1990) 265. 22255-22261 ).

All assays were performed in 20mM HEPES pH 7.5 containing 10mM MgCI₂, 10mM MnCI₂, 0.05% Brij, 1μM ATP (0.5μCi[γ-33p]ATP) and 0.8mg/ml RR-src. Inhibitors in dimethylsulphoxide (DMSO) were added such that the final concentration of DMSO did not exceed 1%, and enzyme [human p56^ick] such that the consumption of ATP was less than 10%. After incubation at 30°C for 15min, the reaction was terminated by the addition of one-third volume of stop reagent (0.25mM EDTA and 33mM ATP in dH2θ). A 15μl aliquot was removed, spotted onto a P-30 filtermat (Wallac, Milton Keynes, UK), and washed sequentially with 1% acetic acid and dH₂0 to remove ATP. The bound 33p.RR._{src was} quantitated by scintillation counting of the filtermat in a Betaplate scintillation counter (Wallac, Milton Keynes, UK) after addition of Meltilex scintillant (Wallac, Milton Keynes, UK). The dpm obtained, being directly proportional to the amount of 33p.RR-src produced by p56^lck, were used to determine the IC50 for each compound.

p59i¥0 kinase assay Compounds of the invention were assayed for pδθ^ inhibitory activity in a similar manner to the p56^{i k} assay, using human pSθty^1"1.

EGFr kinase assay

The tyrosine kinase activity of the EGF receptor (EGFr) was determined using a similar methodology to the p56^lck kinase assay, except that the

RR-src peptide was replaced by a peptide substrate for EGFr obtained from Amersham International pic (Little Chalfont, UK) and used at the manufacturers recommended concentration IC50 values for each test inhibitor were determined as described previously in the p56^lck assay.

ZAP-70 kinase assay

The tyrosine kinase activity of ZAP-70 was determined using a capture assay based on that employed above for p56^lck The RR-src peptide was replaced with polyGlu-Tyr (Sigma, Poole, UK) at a final concentration of 17 μg/ml. After addition of the stopped reaction to the filtermat, trichloroacetic acid 10% (w/v) was employed as the wash reagent instead of acetic acid and a final wash in absolute ethanol was also performed before scintillation counting IC50 values for each test inhibitor were determined as descπbed above in the p56^lck assay

Csk kinase assay

Compounds of the invention were assayed for csk kinase inhibitory activity in a similar manner to the ZAP-70 assay using human csk kinase

Protein kinase C assay Inhibitor activity against protein kinase C (PKC) was determined using PKC obtained from Sigma Chemical Company (Poole, UK) and a commercially available assay system (Amersham International pic, Little Chalfont, UK). Briefly, PKC catalyses the transfer of the γ-phosphate (32p) of ATP to the threonine group on a peptide specific for PKC Phosphorylated peptide is bound to phosphocellulose paper, subsequently quantified by scintillation counting and IC50 values determined as before

In the above assays, compounds according to the invention, including the compounds of the Examples inhibit the protein kinase p56^lck, p59^fyⁿ, ZAP- 70 or protein kinase C at concentrations at which they have little or no effect on the remaining kinases, including EGFr kinase and Csk kinase Thus, for example the compounds of Example 96 and 103 have IC50 values in the p56^lck assay of 215nM and 40nM respectively The compounds are also active against p59^fyⁿ but have IC50 values at least 10x greater against the remaining kinases described above. In another example the compounds of Example 64 and 66 have IC50 values in the ZAP-70 assay of 124nM and 68nM respectively. The compounds are also active in the protein kinase C assay, but have IC₅₀ values at least 10x greater against the remaining kinases. In a further example, the compounds of Examples 48 and 81 have IC₅₀ values in the protein kinase C assay of 22nM and 92nM respectively, but have IC₅₀ values at least 10x greater against the remaining kinases.

Claims

1. A compound of formula (1):

wherein R¹ is a hydrogen or halogen atom or an optionally substituted straight or branched chain alkyl, alkenyl or alkynyl group or a group selected from hydroxyl (-OH), substituted hydroxyl, thiol

(-SH), substituted thiol, amino (-NH2), or substituted amino;

R² and R³, which may be the same or different, is each an optionally substituted straight or branched chain alkyl, alkenyl or alkynyl group;

R⁴ is a hydrogen atom or a straight or branched chain alkyl group;

R⁵ is a hydrogen atom or an optionally substituted straight or branched chain alkyl, alkenyl or alkynyl group;

R⁶ is a hydrogen or halogen atom or an amino (-NH₂), substituted amino, nitro, carboxyl (-CO2H) or esterified carboxyl group or a group

-X¹ -R6a where X¹ is a direct bond or a linker atom or group and R^6a is an optionally substituted straight or branched chain alkyl, alkenyl or alkynyl group;

X is a direct bond or a linker atom or group;

R⁷ is an optionally substituted aliphatic, cycloaliphatic, heteroaliphatic, heterocycloaliphatic, aromatic or heteroaromatic group; and the salts, solvates, hydrates and N-oxides thereof.

2. A compound according to Claim 1 wherein R⁴ is a hydrogen atom.

3. A compound according to Claim 1 or Claim 2 wherein R⁵ and R⁶ is each a hydrogen atom.

4. A compound according to any one of Claims 1 to 3 wherein R is an optionally substituted methoxy group and R² and R³ is each an optionally substituted methyl or ethyl group.

5. A compound according to Claim 4 wherein R¹ is a methoxy group and R² and R³ is each a methyl group.

6. A compound according to any one of the proceding Claims wherein X is a direct bond, a sulphur atom or a -NH- group.

7. A compound according to any one of Claims 1 to 6 wherein R⁷ is an optionally substituted aromatic or heteroaromatic group.

8. A compound according to Claim 7 wherein R⁷ is an optionally substituted phenyl, 2-pyridyl, 3-pyridyl or 4-pyridyl group.

9. A compound which is

N,N'-Bis(3,4,5-Trimethoxyphenyl)-2,4-pyrimidinediamine;

4-(2-(2-Dimethylaminoethylamino)pyridin-5-yl)-N-(3,4,5- trimethoxyphenyl)-2-pyrimidineamine;

4-(2-(4-Hydroxybutylamino)pyridin-5-yl)-N-(3,4,5-trimethoxyphenyl)-2- pyrimidineamine;

4-(3-(3-Aminopropoxy)phenyl)-N-(3,4,5-trimethoxyphenyl)-2- pyrimidineamine;

N4-(3,4-Dimethoxyphenyl)-N2-(3,4,5-trimethoxyphenyl)-2,4- pyrimidinediamine; 4-(4-(2-Hydroxyethoxy)phenyl)-N-(3,4,5-trimethoxyphenyl)-2- pyrimidineamine;

4-(2-(3-(Morpholino)propylamino)pyridin-5-yl)-N-(3,4,5-trimethoxy- phenyl)-2-pyrimidineamine;

4-(2-(2-(1-Methylpyrrolidin-2-yl)ethylamino)pyridin-5-yl)-N-(3,4,5- trimethoxyphenyl)-2-pyrimidineamine; N4-(4-(Ethoxycarbonylmethyl)phenyl)-N2-(3,4,5-tπmethoxyphenyl)-

2,4-pyπmidinediamine;

N4-(4-Hydroxyphenyl)-N2-(3,4,5-trimethoxyphenyl)-2,4-pyπmιdιne- diamine and

N4-(4-(3-Aminopropoxy)phenyl)-N2-(3,4,5-trιmethoxyphenyl)-2,4- pyrimidinediamine; and the salts, solvates, hydrates and N-oxides thereof.

10. A pharmaceutical composition comprising a compound of formula 1

wherein R¹ is a hydrogen or halogen atom or an optionally substituted straight or branched chain alkyl, alkenyl or alkynyl group or a group selected from hydroxyl (-OH), substituted hydroxyl, thiol

(-SH), substituted thiol, ammo (-NH₂), or substituted ammo;

R² and R³, which may be the same or different, is each an optionally substituted straight or branched chain alkyl, alkenyl or alkynyl group;

R⁴ is a hydrogen atom or a straight or branched chain alkyl group;

R⁵ is a hydrogen atom or an optionally substituted straight or branched chain alkyl, alkenyl or alkynyl group;

R⁶ is a hydrogen or halogen atom or an amino (-NH₂), substituted am o, nitro, carboxyl (-C0₂H) or esterified carboxyl group or a group

.χi .R6a _Where X¹ is a direct bond or a linker atom or group and R^6£* is an optionally substituted straight or branched chain alkyl, alkenyl or alkynyl group;

X is a direct bond or a linker atom or group; R⁷ is an optionally substituted aliphatic, cycloaliphatic, heteroaliphatic, heterocycloaliphatic, aromatic or heteroaromatic group; and the salts, solvates, hydrates and N-oxides thereof; together with one or more pharmaceutically acceptable carriers, excipients or diluents.