US20010004530A1 - Apparatus for measuring a migration ability of ameboidally mobile cells - Google Patents
Apparatus for measuring a migration ability of ameboidally mobile cells Download PDFInfo
- Publication number
- US20010004530A1 US20010004530A1 US09/770,713 US77071301A US2001004530A1 US 20010004530 A1 US20010004530 A1 US 20010004530A1 US 77071301 A US77071301 A US 77071301A US 2001004530 A1 US2001004530 A1 US 2001004530A1
- Authority
- US
- United States
- Prior art keywords
- membrane filter
- active substance
- deposit
- vessels
- vessel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
- G01N33/56972—White blood cells
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0681—Filter
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
Definitions
- the present invention relates to an apparatus for measuring the ability of ameboidally mobile cells to migrate.
- the apparatus comprises a deposit of active substance in the form of a plate, a membrane filter disposed above the deposit, and at least one vessel, which is arranged on top of the filter and has a base-side opening. The base-side opening in the vessel bears against the membrane filter.
- Active substances are understood as meaning all substances which promote or inhibit the migration of ameboidally mobile cells.
- the object of the invention is to provide an apparatus for measuring the ability of ameboidally mobile cells to migrate which overcomes the above-noted deficiencies and disadvantages of the prior art devices and methods of this kind, and wherein, on the one hand, the efficiency of the apparatus and its measurement accuracy are increased and, on the other hand, the measurement method is considerably simplified, and the way in which it is carried out is considerably accelerated, thus making it easier to carry out series of measurements.
- an apparatus for measuring an ability of ameboidally mobile cells to migrate comprising:
- a vessel disposed on the membrane filter the vessel having a base formed with a base-side opening for a liquid containing ameboidally mobile cells, the base-side opening in the vessel bearing against the membrane filter;
- the membrane filter having a surface area at least 1 . 6 times as large as an area of the base-side opening
- adhesive areas disposed to connect the deposit of active substance and the membrane filter the adhesive areas amounting at most to substantially 30% of an area over which the deposit of active substance and the membrane filter bear against one another.
- the area of the membrane filter is at least 1.6 times as great as the area of the base opening of the vessel.
- the deposit of active substance and the membrane filter are joined to one another adhesively such that individual areas of adhesive which extend over their surfaces which bear against one another, preferably arranged in the form of a grid, amount to at most 30% of the area of those surfaces of the deposit of active substance and of the membrane filter which bear against one another.
- An device of this nature ensures close contact between the deposit of active substance and the membrane filter while simultaneously allowing liquids and substances dissolved therein to pass through between the deposit of active substance and the membrane filter.
- the deposit of active substance, the membrane filter and the vessel are placed on top of a support plate made of a transparent or translucent material, and the deposit of active substance and the membrane filter are made from a transparent material or a material which can be changed into a transparent state.
- the membrane filter is preferably designed as an at least approximately rectangular plate, to the underside of which a plurality of deposits of active substance are attached, in particular by adhesive bonding, and on which a plurality of vessels for the cells to be analyzed are arrayed, each vessel being assigned its own deposit of active substance. It is possible for the vessels to be arrayed in adjacent rows, the vessels in one row being connected to one another by webs or the like to form units.
- the vessels may be connected to the membrane filter, once again by adhesive bonding.
- This adhesive bonding is preferably formed in such a way that, after the process of migration has ended, it can easily be detached from the membrane filter without damaging the latter.
- the deposits of active substance, the membrane filter and the vessels situated thereon are placed onto an elongate support plate made from a transparent or translucent material, and the deposits of active substance and the membrane filter are made from a transparent material or from a material which can be changed into a transparent state.
- FIG. 1 is a diagrammatic vertical section of a first embodiment of an apparatus according to the invention
- FIG. 2 is a vertical section taken along the line II-II in FIG. 3, and illustrating a second embodiment of an apparatus according to the invention which represents a measurement unit and makes it easier to carry out large series of measurements;
- FIG. 3 is a plan view of the apparatus of FIG. 2.
- FIG. 1 there is seen a support plate 1 on which there is a deposit of active substance 2 .
- a membrane filter 3 which is joined to the deposit of active substance 2 via a plurality of adhesive bonds 4 .
- a tubular vessel 5 Above the membrane filter 3 there is a tubular vessel 5 , into which a liquid 6 has been introduced which contains cells 7 whose ability to migrate is to be measured.
- the membrane filter 3 has an area which is equal to at least 1.6 times the size of the base opening of the vessel 5 .
- the membrane filter 3 exerts suction on the liquid 6 together with the cells 7 contained therein.
- liquid also passes into the deposit of active substance 2 which is arranged beneath the membrane filter 3 , during which process portions of the active substance are dissolved and subsequently diffuse into the membrane filter 3 and the liquid above it.
- the cells 7 are acted on in such a way that their migration into the membrane filter 3 is influenced.
- the area of the membrane filter 3 is at least 1.6 times as large as the base opening in the vessel 5 leads to a significantly stronger suction being exerted on the liquid 6 , together with the cells 7 contained therein, situated in the vessel 5 than would be the case if the membrane filter is of approximately the same size as the base opening of the vessel 5 .
- the cells 7 are brought into contact with the membrane filter 3 more quickly and can penetrate into this filter more rapidly, so that the time required for the measurement is reduced.
- the deposit of active substance 2 is joined to the membrane filter 3 by adhesive bonding so as to bear tightly against it ensures that the active substance is dissolved and then diffuses into the membrane filter 3 and onward into the liquid 6 inside the vessel 5 in a controlled, uniform manner, which is one of the preconditions for the reproducibility and standardization of migration measurements.
- the total area of the bonded surfaces 4 should cover no more than 30% of the surfaces bearing against one another, since otherwise the diffusion of the liquid into the deposit of active substance 2 and, in addition, the diffusion of the dissolved active substance out of the deposit of active substance 2 into the membrane filter 3 would be considerably restricted.
- the individual bonded areas 4 may be arrayed in a grid-like pattern.
- the cells which have migrated into the membrane filter 3 are made visible, for example by staining, and then their number, distribution, and shape are determined by means of a microscopic assessment method. If the components of the apparatus are transparent or can be made transparent, an illumination method by transmitted light, i.e., transillumination, can be employed for this purpose.
- FIGS. 2 and 3 illustrate an apparatus of this type which can be used to facilitate series of measurements.
- a plurality of the components illustrated in FIG. 1 are combined to form one measurement unit, allowing a simple, rapid and clear migration measurement to be carried out.
- the second embodiment of the apparatus comprises a rectangular support plate 11 which is preferably made from a transparent or translucent material.
- a mat-like membrane filter 13 which covers a plurality of deposits of active substance 12 which are spaced apart from one another, is situated on top of this support plate 11 .
- the deposits of active substance 12 are joined to the membrane filter 13 by means of a plurality of adhesive bonds.
- the deposits of active substance 12 and those parts of the membrane filter 13 which do not cover the deposits of active substance 12 are also joined to the support plate 11 by adhesive bonds.
- the individual vessels 15 and 15 a in the two rows are connected to one another by means of webs 18 and 18 a to form units.
- webs 18 and 18 a to form units.
- the deposits of active substance 12 situated beneath the three vessels 15 are laden with an active substance, whereas the deposits of active substance situated beneath the vessels 15 a arranged parallel to the vessels 15 do not contain any active substance, since they are used to measure the unstimulated, spontaneous cell migration.
- the measurement which is carried out in triplicate simultaneously in the exemplary embodiment is used to increase the measurement accuracy.
- the deposits of active substance 12 beneath the vessels 15 and 15 a are each laden with different active substances, so that it is possible to compare the different effects of these substances on the migration of the cells which are to be analyzed.
- the cells which have migrated into the membrane filter 13 are fixed and stained as a result of suitable substances being added to the vessels 15 and 15 a . After preparation has been finished, the vessels 15 and 15 a are detached from the membrane filter 13 . The cells which have migrated into the membrane filter 13 are then accessible for microscopic analysis.
- the vessels 15 and 15 a have an internal diameter of approximately 7 mm and a height of approximately 9 mm.
- the membrane filter 13 and the deposits of active substance 12 are each 140 ⁇ m thick.
- the vessels 15 and 15 a and the support plate 11 can be made of plastic or of glass. The only required criterion is that they be inert with respect to the media used and the cells to be analyzed.
Abstract
An apparatus for measuring the ability of ameboidally mobile cells to migrate has a deposit of active substance in the form of a plate, a membrane filter disposed on the deposit and one or more vessels on the filter. The vessel or vessels have a base opening, for a liquid which contains ameboidally mobile cells. The base opening in the vessel bears against the membrane filter. A surface area of the membrane filter is at least 1.6 times as large as the area of the base opening in the vessel.
Description
- This is a continuation of copending International Application PCT/AT99/00165, filed Jun. 24, 1999, which designated the United States.
- 1. Field of the Invention
- The present invention relates to an apparatus for measuring the ability of ameboidally mobile cells to migrate. The apparatus comprises a deposit of active substance in the form of a plate, a membrane filter disposed above the deposit, and at least one vessel, which is arranged on top of the filter and has a base-side opening. The base-side opening in the vessel bears against the membrane filter.
- Active substances are understood as meaning all substances which promote or inhibit the migration of ameboidally mobile cells.
- Since the ability of ameboidally mobile cells to migrate is an essential characteristic of such cells, it is of considerable interest to theoretical and applied medicine. With regard to the importance of measuring the ability of ameboidally mobile cells to migrate, its application in human medicine and with regard to an apparatus according to the prior art, reference is had to the disclosure in my prior Austrian patent No. AT 394455 B, which is herewith incorporated by reference.
- The object of the invention is to provide an apparatus for measuring the ability of ameboidally mobile cells to migrate which overcomes the above-noted deficiencies and disadvantages of the prior art devices and methods of this kind, and wherein, on the one hand, the efficiency of the apparatus and its measurement accuracy are increased and, on the other hand, the measurement method is considerably simplified, and the way in which it is carried out is considerably accelerated, thus making it easier to carry out series of measurements.
- With the above and other objects in view there is provided, in accordance with the invention, an apparatus for measuring an ability of ameboidally mobile cells to migrate, comprising:
- a plate-shaped deposit of active substance;
- a membrane filter disposed on the deposit;
- a vessel disposed on the membrane filter, the vessel having a base formed with a base-side opening for a liquid containing ameboidally mobile cells, the base-side opening in the vessel bearing against the membrane filter;
- the membrane filter having a surface area at least1.6 times as large as an area of the base-side opening;
- adhesive areas disposed to connect the deposit of active substance and the membrane filter, the adhesive areas amounting at most to substantially 30% of an area over which the deposit of active substance and the membrane filter bear against one another.
- In other words, the area of the membrane filter is at least 1.6 times as great as the area of the base opening of the vessel. Preferably, the deposit of active substance and the membrane filter are joined to one another adhesively such that individual areas of adhesive which extend over their surfaces which bear against one another, preferably arranged in the form of a grid, amount to at most 30% of the area of those surfaces of the deposit of active substance and of the membrane filter which bear against one another. An device of this nature ensures close contact between the deposit of active substance and the membrane filter while simultaneously allowing liquids and substances dissolved therein to pass through between the deposit of active substance and the membrane filter.
- In accordance with an added feature of the invention, the deposit of active substance, the membrane filter and the vessel are placed on top of a support plate made of a transparent or translucent material, and the deposit of active substance and the membrane filter are made from a transparent material or a material which can be changed into a transparent state. This makes it possible to evaluate the ability of the cells to migrate using a microscopic transillumination method, i.e., by transmitted light.
- In order to further facilitate large series of measurements, it is possible for individual measurement devices which, for example, contain different active substances to be combined to form a measurement unit, in which case a multiplicity of the measurement devices may be provided, in order to increase accuracy. For this purpose, the membrane filter is preferably designed as an at least approximately rectangular plate, to the underside of which a plurality of deposits of active substance are attached, in particular by adhesive bonding, and on which a plurality of vessels for the cells to be analyzed are arrayed, each vessel being assigned its own deposit of active substance. It is possible for the vessels to be arrayed in adjacent rows, the vessels in one row being connected to one another by webs or the like to form units.
- In accordance with a concomitant feature of the invention, the vessels may be connected to the membrane filter, once again by adhesive bonding. This adhesive bonding is preferably formed in such a way that, after the process of migration has ended, it can easily be detached from the membrane filter without damaging the latter. Preferably, the deposits of active substance, the membrane filter and the vessels situated thereon are placed onto an elongate support plate made from a transparent or translucent material, and the deposits of active substance and the membrane filter are made from a transparent material or from a material which can be changed into a transparent state.
- Other features which are considered as characteristic for the invention are set forth in the appended claims.
- Although the invention is illustrated and described herein as embodied in an apparatus for measuring the ability of ameboidally mobile cells to migrate, it is nevertheless not intended to be limited to the details shown, since various modifications and structural changes may be made therein without departing from the spirit of the invention and within the scope and range of equivalents of the claims.
- The construction and method of operation of the invention, however, together with additional objects and advantages thereof will be best understood from the following description of specific embodiments when read in connection with the accompanying drawings.
- FIG. 1 is a diagrammatic vertical section of a first embodiment of an apparatus according to the invention;
- FIG. 2 is a vertical section taken along the line II-II in FIG. 3, and illustrating a second embodiment of an apparatus according to the invention which represents a measurement unit and makes it easier to carry out large series of measurements; and
- FIG. 3 is a plan view of the apparatus of FIG. 2.
- Referring now to the figures of the drawing in detail and first, particularly, to FIG. 1 thereof, there is seen a
support plate 1 on which there is a deposit ofactive substance 2. Above the deposit ofactive substance 2 there is amembrane filter 3 which is joined to the deposit ofactive substance 2 via a plurality of adhesive bonds 4. Above themembrane filter 3 there is atubular vessel 5, into which a liquid 6 has been introduced which contains cells 7 whose ability to migrate is to be measured. Themembrane filter 3 has an area which is equal to at least 1.6 times the size of the base opening of thevessel 5. - The
membrane filter 3 exerts suction on the liquid 6 together with the cells 7 contained therein. As a result, liquid also passes into the deposit ofactive substance 2 which is arranged beneath themembrane filter 3, during which process portions of the active substance are dissolved and subsequently diffuse into themembrane filter 3 and the liquid above it. As a result, the cells 7 are acted on in such a way that their migration into themembrane filter 3 is influenced. - The fact that the area of the
membrane filter 3 is at least 1.6 times as large as the base opening in thevessel 5 leads to a significantly stronger suction being exerted on the liquid 6, together with the cells 7 contained therein, situated in thevessel 5 than would be the case if the membrane filter is of approximately the same size as the base opening of thevessel 5. As a result, the cells 7 are brought into contact with themembrane filter 3 more quickly and can penetrate into this filter more rapidly, so that the time required for the measurement is reduced. - Since the ability of some types of cell to migrate may change rapidly outside the organism, a reduced measurement time leads to an improved determination of the diagnostically significant original readiness of the cells to migrate. Therefore, the increased suction provided by the enlarged membrane filter results in significantly more accurate measurement results as compared to the known prior art.
- The fact that the deposit of
active substance 2 is joined to themembrane filter 3 by adhesive bonding so as to bear tightly against it ensures that the active substance is dissolved and then diffuses into themembrane filter 3 and onward into the liquid 6 inside thevessel 5 in a controlled, uniform manner, which is one of the preconditions for the reproducibility and standardization of migration measurements. However, the total area of the bonded surfaces 4 should cover no more than 30% of the surfaces bearing against one another, since otherwise the diffusion of the liquid into the deposit ofactive substance 2 and, in addition, the diffusion of the dissolved active substance out of the deposit ofactive substance 2 into themembrane filter 3 would be considerably restricted. The individual bonded areas 4 may be arrayed in a grid-like pattern. - After the migration process has ended, the cells which have migrated into the
membrane filter 3 are made visible, for example by staining, and then their number, distribution, and shape are determined by means of a microscopic assessment method. If the components of the apparatus are transparent or can be made transparent, an illumination method by transmitted light, i.e., transillumination, can be employed for this purpose. - The basic structure and fundamental function of an apparatus of this type have been described with reference to FIG. 1. By contrast, FIGS. 2 and 3 illustrate an apparatus of this type which can be used to facilitate series of measurements. In this apparatus, a plurality of the components illustrated in FIG. 1 are combined to form one measurement unit, allowing a simple, rapid and clear migration measurement to be carried out.
- The second embodiment of the apparatus comprises a
rectangular support plate 11 which is preferably made from a transparent or translucent material. A mat-like membrane filter 13, which covers a plurality of deposits ofactive substance 12 which are spaced apart from one another, is situated on top of thissupport plate 11. The deposits ofactive substance 12 are joined to themembrane filter 13 by means of a plurality of adhesive bonds. The deposits ofactive substance 12 and those parts of themembrane filter 13 which do not cover the deposits ofactive substance 12 are also joined to thesupport plate 11 by adhesive bonds. - In this exemplary embodiment, two rows of three
vessels liquid 16 containing thecells 17 to be analyzed has been introduced, are situated above themembrane filter 13. Theindividual vessels webs membrane filter 13 together and then adhesively bonded to the filter. In addition, they can be detached from themembrane filter 13 together after the migration process and the preparation of the cells which have migrated into themembrane filter 13. The deposits ofactive substance 12 situated beneath the threevessels 15 are laden with an active substance, whereas the deposits of active substance situated beneath thevessels 15 a arranged parallel to thevessels 15 do not contain any active substance, since they are used to measure the unstimulated, spontaneous cell migration. The measurement which is carried out in triplicate simultaneously in the exemplary embodiment is used to increase the measurement accuracy. - According to another exemplary embodiment, the deposits of
active substance 12 beneath thevessels - According to another method, after the migration process has concluded, the cells which have migrated into the
membrane filter 13 are fixed and stained as a result of suitable substances being added to thevessels vessels membrane filter 13. The cells which have migrated into themembrane filter 13 are then accessible for microscopic analysis. - In one exemplary embodiment, the
vessels membrane filter 13 and the deposits ofactive substance 12 are each 140 μm thick. Thevessels support plate 11 can be made of plastic or of glass. The only required criterion is that they be inert with respect to the media used and the cells to be analyzed.
Claims (12)
1. An apparatus for measuring an ability of ameboidally mobile cells to migrate, comprising:
a plate-shaped deposit of active substance;
a membrane filter disposed on said deposit;
a vessel disposed on said membrane filter, said vessel having a base formed with a base-side opening for a liquid containing ameboidally mobile cells, said base-side opening in said vessel bearing against said membrane filter;
said membrane filter having a surface area at least 1.6 times as large as an area of said base-side opening;
adhesive areas disposed to connect said deposit of active substance and said membrane filter, said adhesive areas amounting at most to substantially 30% of an area over which said deposit of active substance and said membrane filter bear against one another.
2. The apparatus according to , wherein said adhesive areas are a plurality of adhesive areas arranged in form of a grid.
claim 1
3. The apparatus according to , wherein the area of said deposit of active substance is approximately equal to a base area of said vessel.
claim 1
4. The apparatus according to , which comprises a support plate of translucent material carrying said deposit of active substance, said membrane filter, and said vessel, and wherein said deposit of active substance and said membrane filter are made of a transparent material or a material capable of changing into a transparent state.
claim 1
5. The apparatus according to , wherein said support plate is formed of transparent material.
claim 4
6. The apparatus according to , wherein said membrane filter is an at least substantially rectangular plate having an underside with plurality of said deposits of active substance attached thereto, and a top carrying a plurality of vessels arranged in groups of mutually interconnected vessels.
claim 1
7. The apparatus according to , which comprises webs interconnecting said vessels to form units.
claim 6
8. The apparatus according to , wherein said deposits of active substance are adhesively bonded to said underside of said plate.
claim 6
9. The apparatus according to , wherein said vessels are arranged in mutually adjacent rows, and said vessels of each row are connected to one another to form units.
claim 6
10. The apparatus according to , wherein said vessels are adhesively bonded to said membrane filter.
claim 6
11. The apparatus according to , which comprises an elongate support plate of translucent material carrying said deposits of active substance, said membrane filter, and said vessels, and wherein said deposits of active material and said membrane filter are made of a transparent material or a material capable of changing into a transparent state.
claim 6
12. The apparatus according to , wherein said support plate is formed of transparent material.
claim 11
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ATA1584/98 | 1998-09-22 | ||
AT1854/98 | 1998-09-22 | ||
AT0158498A AT406310B (en) | 1998-09-22 | 1998-09-22 | DEVICE FOR MEASURING THE MIGRATION CAPABILITY OF AMÖBOID MOVABLE CELLS |
PCT/AT1999/000165 WO2000017652A1 (en) | 1998-09-22 | 1999-06-24 | Device for measuring migration capability of amoeboid moving cells |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/AT1999/000165 Continuation WO2000017652A1 (en) | 1998-09-22 | 1999-06-24 | Device for measuring migration capability of amoeboid moving cells |
Publications (2)
Publication Number | Publication Date |
---|---|
US20010004530A1 true US20010004530A1 (en) | 2001-06-21 |
US6350610B2 US6350610B2 (en) | 2002-02-26 |
Family
ID=3516584
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US09/770,713 Expired - Fee Related US6350610B2 (en) | 1998-09-22 | 2001-01-25 | Apparatus for measuring a migration ability of ameboidally mobile cells |
Country Status (8)
Country | Link |
---|---|
US (1) | US6350610B2 (en) |
EP (1) | EP1116035A1 (en) |
JP (1) | JP2002525604A (en) |
AT (1) | AT406310B (en) |
AU (1) | AU748439B2 (en) |
CA (1) | CA2336402A1 (en) |
WO (1) | WO2000017652A1 (en) |
ZA (1) | ZA200100179B (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2017024328A1 (en) | 2015-08-12 | 2017-02-16 | Meon Medical Solutions Gmbh & Co Kg | Device and method for determining the migratory ability of cells capable of amoeboid movement |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AT406310B (en) * | 1998-09-22 | 2000-04-25 | Gerd Dr Egger | DEVICE FOR MEASURING THE MIGRATION CAPABILITY OF AMÖBOID MOVABLE CELLS |
FR2792333B1 (en) * | 1999-04-14 | 2003-01-24 | Labonord | DEVICE FOR DEPOSITING CELLS ON AN ANALYSIS PLATE |
US6706520B2 (en) * | 2001-06-13 | 2004-03-16 | Kehan Han | Assessment of invasive potential of tumor cells |
JP6131595B2 (en) * | 2012-12-28 | 2017-05-24 | 株式会社ニコン | Measuring method |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2923669A (en) * | 1954-11-22 | 1960-02-02 | Millipore Filter Corp | Method of bacterial analysis |
US3783105A (en) * | 1971-01-27 | 1974-01-01 | Geomet | Apparatus for assaying enzyme activity |
DE3119269A1 (en) * | 1981-05-14 | 1982-12-02 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen | Method for determining cell-mediated reactivity |
US4693834A (en) * | 1986-05-05 | 1987-09-15 | Murex Corporation | Transverse flow diagnostic kit |
US5081017A (en) * | 1986-02-18 | 1992-01-14 | Texas Bioresource Corporation | Method and apparatus for detection and quantitation of bacteria |
ES2053550T3 (en) * | 1986-08-28 | 1994-08-01 | Unilever Nv | APPARATUS AND METHODS FOR THE CULTIVATION AND TESTING OF MICROORGANISMS. |
EP0334015B1 (en) * | 1988-03-23 | 1994-05-11 | Becton, Dickinson and Company | A device for delivering a fluid sample to a diagnostic device at a controlled rate |
US5135872A (en) * | 1989-04-28 | 1992-08-04 | Sangstat Medical Corporation | Matrix controlled method of delayed fluid delivery for assays |
AT394455B (en) * | 1990-10-30 | 1992-04-10 | Gerd Dr Egger | A membrane filter system for measuring cell migration |
US5141718A (en) * | 1990-10-30 | 1992-08-25 | Millipore Corporation | Test plate apparatus |
AT406310B (en) * | 1998-09-22 | 2000-04-25 | Gerd Dr Egger | DEVICE FOR MEASURING THE MIGRATION CAPABILITY OF AMÖBOID MOVABLE CELLS |
-
1998
- 1998-09-22 AT AT0158498A patent/AT406310B/en not_active IP Right Cessation
-
1999
- 1999-06-24 AU AU44894/99A patent/AU748439B2/en not_active Ceased
- 1999-06-24 JP JP2000571262A patent/JP2002525604A/en active Pending
- 1999-06-24 CA CA002336402A patent/CA2336402A1/en not_active Abandoned
- 1999-06-24 WO PCT/AT1999/000165 patent/WO2000017652A1/en not_active Application Discontinuation
- 1999-06-24 EP EP99927575A patent/EP1116035A1/en not_active Withdrawn
-
2001
- 2001-01-08 ZA ZA200100179A patent/ZA200100179B/en unknown
- 2001-01-25 US US09/770,713 patent/US6350610B2/en not_active Expired - Fee Related
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2017024328A1 (en) | 2015-08-12 | 2017-02-16 | Meon Medical Solutions Gmbh & Co Kg | Device and method for determining the migratory ability of cells capable of amoeboid movement |
US20180229238A1 (en) * | 2015-08-12 | 2018-08-16 | Meon Medical Solutions Gmbh & Co. Kg | Device and Method for Determining the Migration Ability of Amoeboidally Mobile Cells |
US11130133B2 (en) * | 2015-08-12 | 2021-09-28 | Meon Medical Solutions Gmbh & Co. Kg | Device and method for determining the migration ability of amoeboidally mobile cells |
Also Published As
Publication number | Publication date |
---|---|
AT406310B (en) | 2000-04-25 |
ZA200100179B (en) | 2002-07-01 |
ATA158498A (en) | 1999-08-15 |
JP2002525604A (en) | 2002-08-13 |
AU4489499A (en) | 2000-04-10 |
US6350610B2 (en) | 2002-02-26 |
AU748439B2 (en) | 2002-06-06 |
EP1116035A1 (en) | 2001-07-18 |
WO2000017652A1 (en) | 2000-03-30 |
CA2336402A1 (en) | 2000-03-30 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US5650125A (en) | Method and apparatus for conducting tests | |
CA1202870A (en) | System and methods for cell selection | |
EP1444500B1 (en) | Microwell biochip | |
EP0679195B1 (en) | Multiple-site chemotactic test apparatus and method | |
AU744543B2 (en) | Biosensor array comprising cell populations confined to microcavities | |
US4699884A (en) | Process and apparatus for the simultaneous application of a multiplicity of liquid samples to an object stage | |
JPH01308965A (en) | Analyzer and method of causing reaction of sample with object to be analyzed | |
JPH0361857A (en) | Method of obtaining aliquot of matter in distributed form in gelatinous body | |
EP0757921B1 (en) | Liquid holding device | |
US6350610B2 (en) | Apparatus for measuring a migration ability of ameboidally mobile cells | |
EP0574243A2 (en) | Analytical transfer device | |
JPS63502139A (en) | biological diagnostic equipment | |
US9410952B2 (en) | Method and device for the determination of several analytes with simultaneous internal verification in a graphical combination | |
EP1226228A1 (en) | A cell migration and chemotaxis chamber | |
JPH04506899A (en) | Microbial test equipment | |
US20050226784A1 (en) | Instruments for forming an immobilized sample on a porous membrane, and methods for quantifying target substances in immobilized samples | |
DE4120139A1 (en) | Time-saving method for fixed immunoassays in diagnostics - comprises covering pin-surfaces with the substrate to be measured and immersing the pins in plate with complementary cavities | |
US7005008B2 (en) | Reaction vessel | |
DE69920874D1 (en) | METHOD AND DEVICE FOR "HIGH-DENSITY FORMAT SCREENING" FOR FINDING BIOACTIVE COMPOUNDS | |
KR20210086461A (en) | Skin chip, method for manufacturing skin chip and cell culture method using skin chip | |
CN110794128A (en) | Dry chemical method multi-layer membrane reagent for in vitro biochemical diagnosis | |
CN214439214U (en) | ELISA assay enzyme-labeled coating plate placing device | |
JP4113735B2 (en) | Protein solid phase chip and solid phase sample preparation device | |
JPWO2001044814A1 (en) | Void-forming member holding carrier | |
JPH0511502Y2 (en) |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
REMI | Maintenance fee reminder mailed | ||
LAPS | Lapse for failure to pay maintenance fees | ||
LAPS | Lapse for failure to pay maintenance fees |
Free format text: PATENT EXPIRED FOR FAILURE TO PAY MAINTENANCE FEES (ORIGINAL EVENT CODE: EXP.); ENTITY STATUS OF PATENT OWNER: SMALL ENTITY |
|
STCH | Information on status: patent discontinuation |
Free format text: PATENT EXPIRED DUE TO NONPAYMENT OF MAINTENANCE FEES UNDER 37 CFR 1.362 |
|
FP | Lapsed due to failure to pay maintenance fee |
Effective date: 20060226 |