US20040192913A1

US20040192913A1 - Process for the racemization of chiral quinazolinones

Info

Publication number: US20040192913A1
Application number: US10/773,602
Authority: US
Inventors: Gustave Bergnes; Bing Yao; David Morgans; Whitney Smith
Original assignee: Cytokinetics Inc
Current assignee: Cytokinetics Inc
Priority date: 2001-11-20
Filing date: 2004-02-06
Publication date: 2004-09-30
Also published as: US6753428B2; AU2002346471A1; WO2003043995A1; US20030166933A1

Abstract

Racemates are obtained from one of the enantiomers, or an enantiomerically enriched mixture, of an optically active quinazolinone derivative by reaction of the compound with an alkali alkoxide of a primary alcohol and isolation of the racemate.

Description

CROSS REFERENCE TO RELATED APPLICATIONS

This application claims priority under 35 U.S.C. §119(e) to U.S. Ser. No. 60/332,148, filed Nov. 20, 2002, which is incorporated by reference in its entirety for all purposes.[0001]

FIELD OF THE INVENTION

This invention relates to a process for racemizing one of the enantiomers, or an enantiomerically enriched mixture, of an optically active compound. This invention more specifically relates to racemization of quinazolinone derivatives.

BACKGROUND OF THE INVENTION

Interest in the medicinal chemistry of quinazoline derivatives was stimulated in the early 1950's with the elucidation of the structure of a quinazoline alkaloid, 3-[β-keto-γ-(3-hydroxy-2-piperidyl)-propyl]-4-quinazolone, from an Asian plant known for its antimalarial properties. In a quest to find additional antimalarial agents, various substituted quinazolines have been synthesized. Of particular import was the synthesis of the derivative 2-methyl-3-o-tolyl-4-(3H)-quinazolinone. This compound, known by the name methaqualone, though ineffective against protozoa, was found to be a potent hypnotic.

Since the introduction of methaqualone and its discovery as a hypnotic, the pharmacological activity of quinazolinones and related compounds has been investigated. Quinazolinones and derivatives thereof are now known to have a wide variety of biological properties including hypnotic, sedative, analgesic, anticonvulsant, antitussive and anti-inflammatory activities.

Quinazolinones are among a growing number of therapeutic agents used to treat cell proliferative disorders, including cancer. For example, PCT WO 01/30768, which is incorporated herein by reference in its entirety, describes a pharmaceutical composition containing quinazolinone derivatives which are inhibitors of the mitotic kinesin KSP and are useful in the treatment of cellular proliferative diseases, for example cancer, hyperplasias, restenosis, cardiac hypertrophy, immune disorders and inflammation. Certain of the compounds described therein have the following formula:

wherein

R ₁is chosen from hydrogen, alkyl, aryl, aralkyl, heteroaryl, heteroaralkyl, substituted alkyl, substituted aryl, substituted aralkyl, substituted heteroaryl, and substituted heteroaralkyl;

R ₂and R₂′ are independently chosen from hydrogen, alkyl, oxaalkyl, aryl, aralkyl, heteroaryl, heteroaralkyl, substituted alkyl, substituted aryl, substituted aralkyl, substituted heteroaryl, and substituted heteroaralkyl; or R₂and R₂′ taken together form a 3- to 7-membered ring;

R ₃is chosen from hydrogen, alkyl, aryl, aralkyl, heteroaryl, heteroaralkyl, substituted alkyl, substituted aryl, substituted aralkyl, substituted heteroaryl, substituted heteroaralkyl, oxaalkyl, oxaaralkyl, substituted oxaaralkyl, R₁₅O— and R₁₅—NH—;

R _3ais chosen from hydrogen, alkyl, aryl, alkylaryl, heteroaryl, alkylheteroaryl, substituted alkyl, substituted aryl, substituted alkylaryl, substituted heteroaryl, substituted alkylheteroaryl and R₁₅—NH—;

R _3bis chosen from alkyl, aryl, alkylaryl, heteroaryl, alkylheteroaryl, substituted alkyl, substituted aryl, substituted alkylaryl, substituted heteroaryl, and substituted alkylheteroaryl;

R ₄is chosen from hydrogen, alkyl, aryl, aralkyl, heteroaryl, heteroaralkyl, substituted alkyl, substituted aryl, substituted aralkyl, substituted heteroaryl, substituted heteroaralkyl, and R₁₆-alkylene-;

R ₅, R₆, R₇and R₈are independently chosen from hydrogen, alkyl, alkoxy, halogen, fluoroalkyl, nitro, dialkylamino, alkylsulfonyl, alkylsulfonamido, sulfonamidoalkyl, sulfonamidoaryl, alkylthio, carboxyalkyl, carboxamido, aminocarbonyl, aryl and heretoaryl;

R ₁₅is chosen from alkyl, aryl, aralkyl, heteroaryl, heteroaralkyl, substituted alkyl, substituted aryl, substituted aralkyl, substituted heteroaryl, and substituted heteroaralkyl; and

R ₁₆is chosen from alkoxy, amino, alkylamino, dialkylamino, N-heterocyclyl and substituted N-heterocyclyl.

These quinazolinone derivatives have an asymmetric carbon atom (i.e., the stereogenic center to which R ₂and R₂are attached) that may exist as a racemic mixture of these compounds, i.e., a mixture of both the (+) and (−) or dextro and levo rotary forms. These compounds can be produced as racemates and administered in this form. However, it is known that the physiological utility of racemic mixtures often is focused on one enantiomer, the other having either little or no effect or even diminishing the effect of the active enantiomer.

A generic synthetic scheme for the preparation of quinazolinone compounds of Formula I(a)-(d) above is described in PCT WO 01/30768 and is shown in FIGS. 1 and 2. An asymmetric synthesis of the quinazolinone compounds of Formula I using optically active reagents is shown in FIG. 3. Disposal of the undesired enantiomer of an intermediate is not environmentally or economically desirable. Thus an efficient method of converting the inactive or undesirable enantiomer into the other usable, desirable enantiomer is a commercially important objective. This invention makes it possible to achieve this objective.

The references discussed herein are provided solely for their disclosure prior to the filing date of the present application and are each incorporated herein by reference. Nothing herein is to be construed as an admission that the inventors are not entitled to antedate such disclosure by virtue of prior invention.

SUMMARY OF THE INVENTION

The present invention provides a method for racemizing one of the enantiomers, or an enantiomerically enriched mixture, of an optically active compound of the formula:

comprising:

contacting said compound with an alkali alkoxide of a C ₁-C₆primary alcohol; and

isolating the resulting racemic compound,

wherein

R ₂is alkyl, oxaalkyl, aryl, aralkyl, heteroaryl, heteroaralkyl, substituted alkyl, substituted aryl, substituted aralkyl, substituted heteroaryl, and substituted heteroaralkyl; and

R ₅, R₆, R₇and R₈are independently chosen from hydrogen, alkyl, alkoxy, halogen, fluoroalkyl, nitro, dialkylamino, alkylsulfonyl, alkylsulfonamido, sulfonamidoalkyl, sulfonamidoaryl, alkylthio, carboxyalkyl, carboxamido, aminocarbonyl, aryl and heretoaryl.

The process is characterized in that one of the enantiomers, or an enantiomerically enriched mixture, of enantiomers is contacted with an alkali metal alkoxide of a primary alcohol. Preferably, the reaction mixture comprises the primary alcohol from which the alkali metal alkoxide was derived. The racemic compounds can then be isolated by conventional methods.

The alkali metal alkoxide of a primary alcohol will generally be derived from a. primary aliphatic alcohol with 1-6 C-atoms, preferably methanol or ethanol, and more preferably, ethanol. Preferably, the reaction is carried out under reflux temperature.

BRIEF DESCRIPTION OF THE DRAWINGS

This invention will be better understood by reference to the following drawings and description, in which the same reference numerals are used to identify like components. [0029]
FIG. 1 depicts a generic synthetic scheme to make quinazolinone derivatives of Formula I. [0030]
FIG. 2 depicts a synthetic route for the synthesis of quinazolinone derivative [0031]
FIG. 3 depicts a synthetic route to substantially pure single enantiomers. [0032]
FIG. 4 depicts a method for the racemization of quinazolinone derivatives according to one embodiment of the invention.[0033]

DESCRIPTION OF THE PREFERRED EMBODIMENT

General [0034]
Before the present methods are described, it is understood that this invention is not limited to the particular methodology, protocols, and reagents described, as these may vary. It is also to he understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the present invention which will be limited only by the appended claims. [0035]
It must be noted that as used herein and in the appended claims, the singular forms “a”, “an”, and “the” include plural reference unless the context clearly dictates otherwise. [0036]
Unless defined otherwise, all technical and scientific terms used herein have the same meanings as commonly understood by one of ordinary skill in the art to which this invention belongs. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, the preferred methods, devices, and materials are now described. [0037]
Alkyl is intended to include linear, branched, or cyclic hydrocarbon structures and combinations thereof. Lower alkyl refers to alkyl groups of from 1 to 5 carbon atoms. Examples of lower alkyl groups include methyl, ethyl, pro pyl, isopropyl, butyl, s-and t-butyl and the like. Preferred alkyl groups are those of C[0038] ₂₀or below. More preferred alkyl groups are those of C₁₃or below. Cycloalkyl is a subset of alkyl and includes cyclic hydrocarbon groups of from 3 to 13 carbon atoms. Examples of cycloalkyl groups include c-propyl, c-butyl, c-pentyl, norbomyl, and the like. In this application, alkyl refers to alkanyl, alkenyl and alkynyl residues; it is intended to include cyclohexylmethyl, vinyl, allyl, isoprenyl and the like. Alkylene refers to the same residues as alkyl, but having two points of attachment. Examples of alkylene include ethylene (—CH₂CH₂—), propylene (—CH₂CH₂CH₂—), dimethylpropylene (—CH₂C(CH₃)₂CH₂—) and cyclohexylpropylene (—CH₂CH₂CH(C₆H₁₃)—). When an alkyl residue having a specific number of carbons is named, all geometric isomers having that number of carbons are intended to be encompassed; thus, for example, “butyl” is meant to include n-butyl, sec-butyl, isobutyl and t-butyl; “propyl” includes n-propyl and isopropyl.
Alkoxy or alkoxyl refers to groups of from 1 to 8 carbon atoms of a straight, branched, cyclic configuration and combinations thereof attached to the parent structure through an oxygen. Examples include methoxy, ethoxy, propoxy, isopropoxy, cyclopropyloxy, cyclohexyloxy and the like. Lower-alkoxy refers to groups containing one to four carbons. [0039]
Aryl and heteroaryl mean a 5- or 6-membered aromatic or heteroaromatic ring containing 0-3 heteroatoms selected from O, N, or S; a bicyclic 9- or 10-membered aromatic or heteroaromatic ring system containing 0-3 heteroatoms selected from O, N, or S; or a tricyclic 13- or 14-membered aromatic or heteroaromatic ring system containing 0-3 heteroatoms selected from O, N, or S. The aromatic 6- to 14-membered carbocyclic rings include, e.g., benzene, naphthalene, indane, tetralin, and the 5- to 10-membered aromatic heterocyclic rings include, e.g., imidazole, pyridine, indole, thiophene, benzopyranone, thiazole, furan, benzimidazole, quinoline, isoquinoline, quinoxaline, pyrimidine, pyrazine, tetrazole and pyrazole. [0040]
Aralkyl refers to a residue in which an aryl moiety is attached to the parent structure via an alkyl residue. Examples are benzyl, phenethyl, phenylvinyl, phenylallyl and the like. Oxaalkyl and oxaaralkyl refer to alkyl and aralkyl residues in which one or more methylenes have been replaced by oxygen. Examples of oxaalkyl and oxaaralkyl residues are ethoxyethoxyethyl (3,6-dioxaoctyl), benzyloxymethyl and phenoxymethyl; in general, glycol ethers, such as polyethyleneglycol, are intended to be encompassed by this group. [0041]
Heteroaralkyl refers to a residue in which a heteroaryl moiety is attached to the parent structure via an alkyl residue. Examples include furanylmethyl, pyridinylmethyl, pyrimidinylethyl and the like. [0042]
Heterocycle means a cycloalkyl or aryl residue in which one to four of the carbons is replaced by a heteroatom such as oxygen, nitrogen or sulfur. Examples of heterocycles that fall within the scope of the invention include imidazoline, pyrrolidine, pyrazole, pyrrole, indole, quinoline, isoquinoline, tetrahydroisoquinoline, benzofuran, benzodioxan, benzodioxole (commonly referred to as methylenedioxyphenyl, when occurring as a substituent), tetrazole, morpholine, thiazole, pyridine, pyridazine, pyrimidine, thiophene, furan, oxazole, oxazoline, isoxazole, dioxane, tetrahydrofuran and the like. “N-heterocyclyl” refers to a nitrogen-containing heterocycle as a substituent residue. The term heterocyclyl encompasses heteroaryl, which is a subset of heterocyclyl. Examples of N-heterocyclyl residues include 4-morpholinyl, 4-thiomorpholinyl, 1-piperidinyl, 1-pyrrolidinyl, 3-thiazolidinyl, piperazinyl and 4-(3,4-dihydrobenzoxazinyl). Examples of substituted heterocyclyl include 4-methyl-1-piperazinyl and 4-benzyl-1-piperidinyl. [0043]
Substituted alkyl, aryl and heteroaryl refer to alkyl, aryl or heteroaryl wherein H atoms are replaced with alkyl, halogen, hydroxy, alkoxy, alkylenedioxy (e.g. methylenedioxy) fluoroalkyl, carboxy (—COOH), carboalkoxy (i.e. acyloxy RCOO—), carboxyalkyl (—COOR), carboxamido, sulfonamidoalkyl, sulfonamidoaryl, aminocarbonyl, benzyloxycarbonylamino (CBZ-amino), cyano, carbonyl, nitro, dialkylamino, alkylamino, amino, alkylthio, alkylsulfinyl, alkylsulfonyl, alkylsulfonamido, arylthio, arylsulfinyl, arylsulfonyl, amidino, phenyl, benzyl, heteroaryl, heterocyclyl, phenoxy, benzyloxy, or heteroaryloxy. For the purposes of the present invention, substituted alkyl also includes oxaalkyl residues, i.e. alkyl residues in which one or more carbons has been replaced by oxygen. [0044]
Halogen refers to fluorine, chlorine, bromine or iodine. Fluorine, chlorine and bromine are preferred. Dihaloaryl, dihaloalkyl, trihaloaryl etc. refer to aryl and alkyl substituted with a plurality of halogens, but not necessarily a plurality of the same halogen; thus 4-chloro-3-fluorophenyl is within the scope of dihaloaryl. [0045]
Starting Materials [0046]
In a preferred embodiment, an enantiomer, or an enantiomerically enriched mixture, of an optically active compound will have a formula: [0047]
wherein[0048]
R[0049] ₁is chosen from hydrogen, alkyl, aryl, aralkyl, heteroaryl, heteroaralkyl, substituted alkyl, substituted aryl, substituted aralkyl, substituted heteroaryl, and substituted heteroaralkyl;
R[0050] ₂is alkyl, oxaalkyl, aryl, aralkyl, heteroaryl, heteroaralkyl, substituted alkyl, substituted aryl, substituted aralkyl, substituted heteroaryl, and substituted heteroaralkyl; and
R[0051] ₅, R₆, R₇and R₈are independently chosen from hydrogen, alkyl, alkoxy, halogen, fluoroalkyl, nitro, cyano, dialkylamino, alkylsulfonyl, alkylsulfonamido, sulfonamidoalkyl, sulfonamidoaryl, alkylthio, carboxyalkyl, carboxamido, aminocarbonyl, aryl and heretoaryl. See, PCT WO 01/30768.
These compounds contain one or more asymmetric centers (e.g. the carbon to which R[0052] ₂is attached) and may thus give rise to enantiomers, diastereomers, and other stereoisomeric forms that may be defined, in terms of absolute stereochemistry, as (R)— or (S)—. The quinazolinones of Formula II which are enriched in one optical isomer may be used in the present invention irrespective of the degree of enantiomeric excess of a specific optical isomer contained therein. Thus, the starting material of the racemization method described herein may be enriched with the R-configuration or the S-configuration. Preferably, it will be enriched with the S-isomer.
Preferably, R[0053] ₁is selected from hydrogen, alkyl, aryl, substituted alkyl, substituted aryl, heteroaryl, substituted heteroaryl, aralkyl, substituted aralkyl, and substituted heteroaralkyl.
In a more preferred embodiment R[0054] ₁is selected from hydrogen, lower alkyl, substituted lower alkyl, aryl, substituted aryl, aralkyl and substituted aralkyl. In a most preferred embodiment R₁is chosen from hydrogen, ethyl, propyl, methoxyethyl, naphthyl, phenyl, bromophenyl, chlorophenyl, methoxyphenyl, ethoxyphenyl, tolyl, dimethylphenyl, chorofluorophenyl, methylchlorophenyl, ethylphenyl, phenethyl, benzyl, chlorobenzyl, methylbenzyl, methoxybenzyl, tetrahydrofuranylmethyl and (ethoxycarbonyl)ethyl.
In a preferred embodiment R[0055] ₂is alkyl or substituted alkyl. In a most preferred embodiment R₂is chosen from methyl, ethyl, propyl, methylthioethyl, aminobutyl, (CBZ)aminobutyl, cyclohexylmethyl, benzyloxymethyl, methylsulfinylethyl, methylsulfinylmethyl, hydroxymethyl, benzyl and indolylmethyl.
Preferably, R[0056] ₅, R₆, R₇and R₈are independently chosen from hydrogen, halogen, methyl, cyano, and trifluoromethyl. More preferably, R₅is hydrogen or halogen; R₆, is hydrogen, methyl, or halogen; R₇is hydrogen, halogen, lower alkyl, substituted lower alkyl, lower alkoxy, or cyano; and/or R₈is hydrogen or halogen. In another embodiment, embodiment, R₅, R₆, and R₈are hydrogen. More preferably, R₅, R₆, and R₈are hydrogen and R₇is halogen
In a particularly preferred subgenus, R[0057] ₁is chosen from aralkyl or substituted aralkyl; R₂is lower alkyl; R₅is hydrogen; R₆is hydrogen; R₇is halo or cyano; and R₈is hydrogen; or R₁is benzyl or substituted benzyl; R₂is i-propyl; R₅is hydrogen; R₆is hydrogen; R₇is chloro or cyano; and R₈is hydrogen; or R₁is benzyl; R₂is i-propyl; R₅is hydrogen; R₆is hydrogen; R₇is fluoro; and R₈is hydrogen, or R₁is benzyl; R₂is i-propyl; R₅is hydrogen; R₆is hydrogen; R₇is chloro; and R₈is hydrogen; or R₁is benzyl; R₂is i-propyl; R₅is hydrogen; R₆is hydrogen; R₇is cyano; and R₈is hydrogen; or R₁is benzyl or halobenzyl; R₂is chosen from ethyl and propyl; R₅is hydrogen; R₆is hydrogen; R₇is halo; and R₈is hydrogen.
The Racemization Methods [0058]
In the racemization method of the present invention, racemization produces a racemic mixture, which can be separated by conventional means such as column chromatography, optical resolution using an optically active acid and the like. [0059]
The methods of the present invention utilize an alkali alkoxide of a C[0060] ₁-C₆primary alcohol to effect the racemization. Preferably, the alkali alkoxide will comprise a sodium or potassium alkoxide. More preferably, it will comprise sodium ethoxide.
The amount of alkali alkoxide used is not particularly limited, but is usually from about 0.01 to 10 moles, preferably 0.01 to 5 moles, more preferably 0.5 to 2 moles per mole of the quinazolinone derivative. Most preferably, equimolar amounts of the alkali alkoxide and the quinazolinone will be used. [0061]
Preferably, a C[0062] ₁-C₆primary alcohol will be used as the solvent. More preferably, the solvent will comprise the alcohol from which the alkali alkoxide was derived. Most preferably, the primary alcohol will be methanol or ethanol. Preferably, the racemization reaction will be conducted in anhydrous conditions.
The reaction mixture may comprise lesser amounts of other solvent including aromatic compounds such as benzene, toluene, ethyl benzene, xylene, and chlorobenzene; hydrocarbons such as hexane, cyclohexane, heptane and isooctane; ethers such as t-butyl methyl ether, isopropyl ether, tetrahydrofuran and dioxane, and mixtures thereof. [0063]
The concentration of starting material in the racemization reaction may be varied. In general, the reaction will be conducted at a concentration of about 0.1 to about 5 molar, more preferably at about 0.5 to about 2 molar, and most preferably at about 0.5 molar. [0064]
The racemization reaction usually will be conducted at a temperature of less than 200° C.; more preferably, at a temperature of less than 100° C.; and most preferably, at the reflux (or boiling point) temperature of the reaction mixture. [0065]
The product of the racemization reaction (i.e., the “racemates”) will be less enriched in one optical isomer and thus, will have a decreased degree of enantiomeric excess as compared to the starting material. Preferably, the racemates will be present in about a 1:1 ratio. [0066]
The racemized quinazolinone derivative thus obtained by the present invention can be reused in an optical resolution process to produce the desired enantiomer of the optically active quinazolinone derivative after being subjected to a conventional procedure, for example, hydrolysis or neutralization with acidic water or the like, followed by distillation of the solvent, if necessary. [0067]
Synthesis of Compounds of Formula II [0068]
The compounds of Formula II can be prepared by following the procedures described with reference to the [0069] Reaction Scheme 1 below and by the procedures set forth in the figures and PCT WO 01/30768.
Preparation of Formula 103 [0070]
Referring to [0071] Reaction Scheme 1, Step 1, to an optionally substituted benzoic acid (the compound of Formula 101) dissolved in an inert organic solvent (such as THF) in the presence of sodium bicarbonate and a dehydrating agent (such as Na₂SO₄) is added a slight molar excess of an optionally substituted acid chloride (the compound of Formula 102), maintaining about room temperature. Completion of the reaction is monitored, e.g., by TLC. Acetic anhydride is then added to the reaction mixture, which is heated to about 90-100° C., monitoring completion of the reaction (e.g., by TLC) followed by removal of the acetic anhydride under vacuum at about 80-100° C. The reaction mixture is cooled and the corresponding, optionally substituted benzo[d][1,3]oxazin-4-one (the compound of Formula 103) is isolated and purified.
Preparation of Formula 104 [0072]
Referring to [0073] Reaction Scheme 1, Step 2, about 1.5 molar equivalents of a primary amine (such as R₁NH₂) and 1 molar equivalent of a compound of Formula 103 in an inert organic solvent (such as toluene) are heated to reflux. The reaction takes place over a period of 1 to 5 hours. After removal of water, ethylene glycol and sodium hydroxide are added to the reaction mixture and the temperature raised to 110-120° C. Completion of the reaction is monitored, e.g., by TLC. The corresponding, optionally substituted quinazolinone (a compound of Formula 104) is isolated and purified.
Preparation of Formula 105 [0074]
Referring to [0075] Reaction Scheme 1, Step 3, a compound of Formula 104, dissolved in acetic acid and in the presence of sodium acetate, is heated to 30° C., followed by the addition (with agitation) of a slight molar excess of bromine in acetic acid over a period of 2.5 hours. Completion is monitored, e.g., by TLC; if the starting material continues to be present, temperature is increased to 50° C. until completion. The corresponding, optionally substituted quinazolinone of Formula 105 is isolated and purified.
Preparation of Formula 106 [0076]
Referring to [0077] Reaction Scheme 1, Step 4, to 1.5 molar equivalents of sodium azide in an inert organic solvent (such as DMF) is slowly added 1 molar equivalent of a compound of Formula 105. The reaction takes place with agitation at a temperature of 40° C. over a period of 3 to 10 hours. Completion is monitored, e.g., by TLC. The corresponding, optionally substituted quinazolinone azide of Formula 106 is isolated and purified.
Preparation of Formula 107 [0078]
Referring to [0079] Reaction Scheme 1, Step 5, to a solution of triphenylphosphine dissolved in an inert organic solvent (such as THF) is added an azide of Formula 106, portionwise over about 15 minutes. The reaction takes place with agitation, maintaining the temperature at 20° C. over a period of 5 minutes to 1 hour. The reaction mixture is acidified, solvents removed followed by conventional work up to give the hydrochloride salt of the corresponding, optionally substituted quinazolinone of Formula 107, which is isolated and purified in the usual manner.
Preparation of Formula 107a [0080]
In certain compounds of the invention, particular stereoconfiguration can be preferred for the R[0081] ²substituent, such as the (R) isomer, which can be obtained, e.g., as illustrated in optional Step 5a of Reaction Scheme 1. An amine of Formula 107 is dissolved in an inert organic solvent (such as IPA) and warmed to 60° C. In a separate vessel, a resolving agent (such as dibenzoyl-D-tartaric acid) is dissolved, preferably in the same warm solvent, and then quickly added (with agitation) to the warm amine solution. The reaction mixture is left to crystallize by cooling to room temperature over 16 hours under continuing agitation. The desired isomer, e.g., the (R) isomer illustrated as Formula 107a, is isolated and purified in the usual manner.
Synthesis of Compounds of Formula I [0082]
The quinazolinone derivative of Formula II can be converted to compounds of Formula I(a), I(b), I(c), or I(d) as described in the Reaction Schemes below and as shown in the figures. See, also, PCT WO 01/30768. [0083]
Preraration of Formula 203 [0084]
Refering to [0085] Reaction Scheme 2, Step 1, to a solution of a compound of Formula 107 is added successively a slight excess (preferably about 1.2 equivalents) of an aldehyde comprising R₄(i.e., a compound having the formula R₄CHO where R₄CH₂— is equivalent to R₄and R₄is as described above or is a protected precursor to such a substituent, e.g., (3-oxo-propyl)-carbamic acid tert-butyl ester) and a reducing agent such as sodium triacetoxyborohydride. The resulting mixture is stirred for several hours. The product, a compound of Formula 203 is isolated and purified.
Preparation of Formula 205 [0086]
Referring to [0087] Reaction Scheme 2, Step 2, to a solution of a compound of Formula 203 and an amine base such as diisopropylethylamine in a nonpolar, aprotic solvent such as dichloromethane is added an R₃acyl chloride (such as Cl—C(O)—R₃where R₃is as described above). The resulting solution is stirred under nitrogen at room temperature for several hours. The product, a compound of Formula 205 is isolated and purified.
Preparation of Formula 207 [0088]
Optionally, any protecting groups on compounds of Formula 205 are then removed. For example, if R[0089] ₄comprises a protected amine wherein the protecting group is a Boc group, the Boc group can be removed by treatment of the compound of Formula 205 with an acid such as trifluoroacetic acid in a nonpolar, aprotic solvent such as dichloromethane, while maintaining the reaction at about room temperature. The reaction is monitored e.g., by TLC. Upon completion, the product, a compound of Formula 207 is isolated and purified.
Referring to [0090] Reaction Scheme 3, to a solution of a compound of Formula 203 and an amine base such as diisopropylethylamine in a nonpolar, aprotic solvent such as dichloromethane is added a compound having the formula Cl—S(O)₂—R_3aor O—(S(O)₂—R_3a)₂where R_3ais as described above. The resulting solution is stirred under nitrogen at room temperature for several hours. The product, a compound of Formula 303 is isolated and purified.
Referring to [0091] Reaction Scheme 4, to a solution of a compound of Formula 203 and an amine base such as diisopropylethylamine in a nonpolar, aprotic solvent such as dichloromethane is added a compound having the formula Cl—CH₂—R_3bwhere R_3bis as described above. The resulting solution is stirred under nitrogen at room temperature or with heat for several hours. The product, a compound of Formula 403 is isolated and purified.
Referring to [0092] Reaction Scheme 5, a compound of Formula 203 is reacted with a slight excess of a compound of the formula R₁₅O(CO)Cl in the presence of a base such as triethylamine in a nonpolar, aprotic solvent such as dichloromethane. The product, a compound of Formula 503 is isolated and purified.
Referring to [0093] Reaction Scheme 6, a compound of Formula 203 is treated with a slight excess of an isocyanate R₁₅—N═C═O in the presence of a base, such as triethylamine, in a nonpolar, aprotic solvent, such as dichloromethane. The product, a compound of Formula 603, is isolated and purified.

EXAMPLES

Abbreviations and Definitions

The following examples are given to enable those skilled in the art to more clearly understand and to practice the present invention. They should not be considered as limiting the scope of the invention, but merely as being illustrative and representative thereof. [0094]
The following abbreviations and terms have the indicated meanings throughout:[0095]
Ac=acetyl [0096]
Boc=t-butyloxy carbonyl [0097]
Bu=butyl [0098]
c-=cyclo [0099]
CBZ=carbobenzoxy=benzyloxycarbonyl [0100]
DBU=diazabicyclo[5.4.0]undec-7-ene [0101]
DCM=dichloromethane=methylene chloride=CH[0102] ₂Cl₂
DCE=dichloroethylene [0103]
DEAD=diethyl azodicarboxylate [0104]
DIC=diisopropylcarbodiimide [0105]
DIEA=N,N-diisopropylethyl amine [0106]
DMAP=4-N,N-dimethylaminopyridine [0107]
DMF=N,N-dimethylformamide [0108]
DMSO=dimethyl sulfoxide [0109]
DVB=1,4-divinylbenzene [0110]
EEDQ=2-ethoxy-1-ethoxycarbonyl-1,2-dihydroquinoline [0111]
Et=ethyl [0112]
Fmoc=9-fluorenylmethoxycarbonyl [0113]
GC=gas chromatography [0114]
HATU=O-(7-Azabenzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate [0115]
HMDS=hexamethyldisilazane [0116]
HOAc=acetic acid [0117]
HOBt=hydroxybenzotriazole [0118]
Me=methyl [0119]
mesyl=methanesulfonyl [0120]
MTBE=methyl t-butyl ether [0121]
NMO=N-methylmorpholine oxide [0122]
PEG=polyethylene glycol [0123]
Ph=phenyl [0124]
PhOH=phenol [0125]
PfP=pentafluorophenol [0126]
PPTS=pyridinium p-toluenesulfonate [0127]
Py=pyridine [0128]
PyBroP=bromo-tris-pyrrolidino-phosphonium hexafluorophosphate [0129]
rt=room temperature [0130]
sat=d=saturated [0131]
s-=secondary [0132]
t-=tertiary [0133]
TBDMS=t-butyldimethylsilyl [0134]
TES=triethylsilane [0135]
TFA=trifluoroacetic acid [0136]
THF=tetrahydrofuran [0137]
TMOF=trimethyl orthoformate [0138]
TMS=trimethylsilyl [0139]
tosyl=p-toluenesulfonyl [0140]
Trt=triphenylmethyl [0141]

Example 1

Synthesis of Compounds

The general synthesis is shown in FIGS. 1 and 2 and is described further above. [0142]
Step 1: N-butyryl Anthranilic Acid. [0143]
To a three-necked, 500 mL round-bottom flask equipped with a thermometer, dropping funnel, and an efficient magnetic stir bar, was added anthranilic acid (1) (0.5 mole, 68.5 g) and dimethyl formamide (250 mL). To this solution was added butyryl chloride (0.55 mole, 57.1 mL) dropwise at such a rate that the temperature of the mixture did not rise above 40° C. The suspension was stirred vigorously at room temperature for at least an additional 3 h. The mixture was poured into water (2000 mL) and stirred for another 1 h. The precipitated product was collected by filtration, washed with cold water, and dried under reduced pressure over P[0144] ₂O₅, yielding compound 2 (67.3 g, 65%).
Step 2: 2-Propyl-3,1-[4H]benzoxazin-4-one. [0145]
Compound 2 (51.8 g, 0.25 mole) was dissolved in acetic anhydride (180 mL) in a 500 mL round-bottom flask equipped with a magnetic stir bar, a Claisen-distillation head (with vacuum inlet) and a thermometer. The flask was placed in an oil bath and slowly heated to 170-180° C. with vigorous stirring. The acetic acid produced was slowly distilled off under atmospheric pressure. Monitoring the head temperature of the distillation unit was used to follow the progress of the transformation. The reaction mixture was then cooled to 60° C. and the excess of acetic anhydride removed by distillation under reduced pressure (ca. 20 mm Hg). The residue was afterward cooled and the product crystallized. The product was triturated with n-hexane (75 mL) and isolated by filtration to yield 2-propyl-3,1-[4H]benzoxazin-4-one (3) (29.3 g, 62%). The above procedure gave [0146] compound 3 sufficiently pure to use directly in the next step.
Step 3: 2-Propyl-3-benzylquinazolin4-one. [0147]
Compound 3 (28.4 g, 0.15 mole) and benzylamine (17.5 mL, 0.16 mole) were refluxed in chloroform (50 ml) in a one-neck 250 mL round-bottom flask for 6 h. After complete consumption of [0148] compound 3, the chloroform was evaporated under reduced pressure. Ethylene glycol (100 mL) and NaOH pellets (0.60 g) were added to the residue and the flask equipped with a Claisen-distillation head and a magnetic stir bar. The flask was immersed in an oil bath and reheated to 130-140° C. bath temperature with vigorous stirring and maintained there for 5 h while the water produced was removed by distillation. After completion of the reaction, the clear solution was allowed to cool to room temperature and kept overnight to precipitate the product. The pH of the suspension was adjusted to 7-8 by adding 3% aq. HCl, the crystals were filtered off and washed with cold water, and then recrystallized from isopropanol (or alternatively from acetone) to provide the compound, 2-propyl-3-benzylquinazolin-4-one (compound 4) (28.0 g, 67%).
Step 4: 2-(1′-bromopropyl)-3-benzylquinazolin-4-one. [0149]
To a three-neck 250 mL round-bottom flask equipped with a thermometer, dropping funnel, and efficient magnetic stir bar was added compound 4 (27.8 g, 0. 10 mole), anhydrous sodium acetate (10.0 g) and glacial acetic acid (130 mL). Bromine (16.0 g, 0.10 mole) dissolved in acetic acid (10 mL) was added dropwise to the above solution at 40 ° C. for 1-2 h. After addition was complete, the mixture was poured into water (1500 mL) and stirred for 1-2 h at room temperature. The precipitated product, 2-(1′-bromopropyl)-3-benzylquinazolin-4-one (5) was isolated by filtration, washed with warm water to remove traces of acetic acid, and rinsed with a small amount of isopropanol. Drying yielded compound 5 (33.0 g, 92%). [0150]
Step 5: 2-[1′-(N,N-dimethylethylenediamino)propyl]-3-benzylquinazolin-4-one. [0151]
Compound 5 (10.7 g, 0.03 mole) and N,N-dimethylethylenediamine (6.6 mL, 0.06 mole) were dissolved in abs. ethanol (60 mL) and heated at reflux for 6 h. After completion of the reaction, the solvent was evaporated under reduced pressure. The residue was dissolved in dichloromethane (150 mL) and washed with 3% aq. NaOH solution (ca. 10-20 mL). The organic layer was dried over MgSO[0152] ₄and evaporated to dryness under reduced pressure. The remaining oily product was purified by flash chromatography on a short silica gel pad using an eluent of CHCl₃—MeOH-aq.NH₃, 90:10:0.1, to give the desired compound (5), 2-[1′-(N,N-dimethylethylenediamino)propyl]-3-benzylquinazolin-4-one (6) (6.0 g, 55%).
Step 6: 2-[1′-(N-4-fluorobenzoyl)-(N,N-dimethylethylenediamino)propyl]-3-benzylquinazolin4-one. [0153]
A stock solution of compound 5 (1.822 g, 5.0 mmol) was prepared in HPLC grade CHCl[0154] ₃(0.5 mL). A stock solution of p-fluorobenzoyl chloride (160.2 mg, 1 mmol) in HPLC grade 1,2-dichloroethane (2.0 mL) was prepared in a 2.0 mL volumetric flask. A third solution of triethylamine (2.0 mL of 0.5 M) was prepared in HPLC grade 1,2-dichlorethane. A 100 μL aliquot of each solution was pipetted into a glass reaction vessel using a Beckman Biomet 2000 automated liquid dispenser. The reaction mixture was shaken using a mechanical shaker, sonicated in an ultrasonic water bath, and then incubated overnight at room temperature. The mixture was diluted in CHCl₃(300 μL) and washed with 5% aqueous NaHCO₃and water. The solvent was removed in vacuo to provide compound 6 (65%). The purity of the compound was analyzed by TLC eluted with CH₂Cl₂-ethanol-concentrated aqueous NH₃, 100:10:1.

Example 2

The following two compounds were synthesized as single enantiomers by the route shown in FIG. 3. [0155]

Example 3

Racemization Method

The S-isomer of the compound shown in FIG. 4, wherein R[0156] ₁is benzyl; R₂is isopropyl; R₅, R₆, and R₈are hydrogen; and R₇is chloro, (490 mg, 1.44 mmol) and sodium ethoxide (0.535 mL of a 21% by weight solution in denatured alcohol containing 5% toluene; 1.64 mmol) were dissolved in abs. ethanol (2.5 mL) and heated at reflux for 36 h. After completion of the reaction, the solvent was evaporated under reduced pressure. The residue was dissolved in dichloromethane and washed with 2N aq. HCl solution which was added dropwise until the solution had a pH of about 7. The organic layer was dried over Na₂SO₄and evaporated to dryness under reduced pressure. The remaining product (478 mg, 86% pure by NMR) comprised a 1:1.1 mixture of the R- and S-isomers as shown by chiral liquid chromatography.
While the present invention has been described with reference to the specific embodiments thereof, it should be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the true spirit and scope of the invention. In addition, many modifications may be made to adapt a particular situation, material, composition of matter, process, process step or steps, to the objective, spirit and scope of the present invention. All such modifications are intended to be within the scope of the claims appended hereto. All patents and publications cited above are hereby incorporated by reference. [0157]

Claims

1-13. (canceled)

14. A method for making an enantiomer, or an enantiomerically enriched mixture of a compound of the formula

wherein said method comprises the steps of:

contacting an enantiomer, or an enantiomerically enriched mixture of a starting compound of formula

with an alkali alkoxide in a C₁-C₆primary alcohol at a selected racemization reaction temperature whereby said enantiomer or enantiomerically enriched mixture is racemized to yield a racemic mixture;

isolating the racemic mixture;

subjecting said racemic mixture to an optical resolution process whereby the racemic mixture is separated into its corresponding stereoisomers; and

converting desired stereoisomer to said compound using an acid chloride of the formula R₃—C(O)—Cl

wherein

R₁is selected from the group consisting of hydrogen, alkyl, aryl, alkylaryl, heteroaryl, alkylheteroaryl, substituted alkyl, substituted aryl, substituted alkylaryl, substituted heteroaryl, and substituted alkylheteroaryl;

R₂and R_2′ are independently selected from the group consisting of alkyl, oxaalkyl, aryl, alkylaryl, heteroaryl, alkylheteroaryl, substituted alkyl, substituted aryl, substituted alkylaryl, substituted heteroaryl, and substituted alkylheteroaryl;

R₃is selected from the group consisting of hydrogen, alkyl, aryl, aralkyl, heteroaryl, heteroaralkyl, substituted alkyl, substituted aryl, substituted aralkyl, substituted heteroaryl, substituted heteroaralkyl, oxaalkyl, oxaaralkyl, substituted oxaaralkyl, R₁₅O— and R₁₅—NH—;

R₄is selected from the group consisting of hydrogen, alkyl, aryl, aralkyl, heteroaryl, heteroaralkyl, substituted alkyl, substituted aryl, substituted aralkyl, substituted heteroaryl, substituted heteroaralkyl, and R₁₆-alkylene-;

R₅, R₆, R₇, R₈are independently selected from the group consisting of hydrogen, alkyl, alkoxy, halogen, fluoralkyl, nitro, dialkylamino, alkylsulfonyl, alkylsulfonamido, sulfonamidoalkyl, sulfonamidoaryl, alkylthio, carboxyalkyl, carboxamido, aminocarbonyl, aryl and heteroaryl;

R₁₅is selected from the group consisting of alkyl, aryl, aralkyl, heteroaryl, heteroaralkyl, substituted alkyl, substituted aryl, substituted aralkyl, substituted heteroaryl, and substituted heteroaralkyl; and

R₁₆is selected from the group consisting of alkoxy, amino, alkylamino, dialkylamino, N-heterocyclyl and substituted heterocyclyl.

15. The method of claim 14, wherein the C₁-C₆primary alcohol is ethanol.

16. The method of claim 14, wherein the alkali alkoxide is a sodium or potassium alkoxide.

17. The method of claim 14, wherein the alkali alkoxide is sodium ethoxide.

18. The method of claim 14, wherein the racemization reaction temperature is less than 200° C.

19. The method of claim 14, wherein the racemization reaction temperature is less than 100° C.

20. The method of claim 14, wherein the racemization reaction temperature is at the boiling point of the reaction mixture.

21. The method of claim 14, wherein the enantiomer has an R-configuration.

22. The method of claim 14, wherein the enantiomer has an S-configuration.

23. The method of claim 14, wherein the alkali alkoxide is derived from an alkali metal and a C₁-C₆primary alcohol.

24. The method of claim 23, wherein the C₁-C₆primary alcohol is methanol or ethanol.