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Publication numberUS2619448 A
Publication typeGrant
Publication dateNov 25, 1952
Filing dateJan 20, 1950
Priority dateJan 21, 1949
Publication numberUS 2619448 A, US 2619448A, US-A-2619448, US2619448 A, US2619448A
InventorsLarsen Gustav
Original AssigneeLarsen Gustav
Export CitationBiBTeX, EndNote, RefMan
External Links: USPTO, USPTO Assignment, Espacenet
Containers for preservation of bacteria cultures
US 2619448 A
Abstract  available in
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Claims  available in
Description  (OCR text may contain errors)

NOV. 25, 1952 S N 26l9448 CONTAINERS FOR PRESERVATION OF BACTERIA CULTURES Filed Jan. 20, 1950 v INVENTOR. Gasiay [arsen Patented Nov. 25, 1952 CONTAINERS FOR PRESERVATION F BACTERIA CULTURES Gustav Larsen, Haslev, Denmark Application January 20, 1950, Serial No. 139,760 In Denmark January 21, 1949 4.- Claims.

The invention relates to improvements in containers for preservation of bacteria cultures, especially for the destruction of vermin such as rats and mice.

The object of the invention is to provide a container of the kind mentioned in which the bacteria culture may be preserved for a long time and easily be transplanted to a substratum for further development in view of subsequent use.

Another object of the invention is to provide a container which ensures an absolutely sterilized handling of the bacteria culture until it is taken out of the container for use.

A further object of the invention is to provide a container consisting of simple means to be produced at low costs, and which container does not necessitate intricate operations to be done when the bacteria are to be transplanted to the substratum.

These and further objects and advantages of the invention will be apparent from the following detailed specification.

The accompanying drawing illustrates a preferred embodiment of the invention.

In the drawing the container is a bottle I filled with a substratum 2, partly shown in dotted lines in the figure, e. g. a peptone-bouillon solution. Furthermore the bottle contains ampule 3 of a transparent glass having such internal tensions that it will crush to dust on being broken and having an external narrow pass 4. The ampule contains a bacteria culture, e. g. of the Salmonella typhi-murium well known for destruction of rats and mice.

Around the narrow pass 4 there is a thread or wire 5 which while keeping the container sterilized is taken out through the sealing of the container, which consists of a sterilized closing plug 6. In this way the bacteria culture can be preserved for several years, and shortly before its use the ampule 3 is broken by pulling the thread or wire 5 whereby the ends of the ampule first contact the shoulders I of the bottle and then the ampule is broken so that its content is poured out into the substratum 2 where it disperses and starts development without the container having been opened.

One day or two later the whole contents of the bottle are useful for destruction of rats and mice during the following month.

When breaking the ampule the contents of its two halves will quickly disperse in the substratum 2. While ordinary glass may be used, this dispersion will be accelerated if the ampule consists of glass having such internal tensions that it is crushed to dust when broken. This property may be imparted to the glass by quickly cooling the molten glass article as by dipping it in water.

It is to be understood that the form of my invention, herewith shown and described, is to be taken as a preferred example of the same, and

2 that various changes are possible within the scope of the claims, without departing from the spirit Of my invention.

What I claim and desire .to secure by Letters Patent is:

1. A container for the preservation of bacteria culture comprising a bottle having a narrow neck portion and a sterile closure, an ampule within said bottle enclosing a bacteria culture, said ampule having an external narrow pass in the middle thereof, a thread or wire extending through said closure and around said narrow pass in such manner as to hold said ampule in a transverse position near the narrow neck of the bottle so that when said thread is pulled said ampule will break against the neck of said bottle.

2. Container for the preservation of bacteria cultures, said container having a sterile closure, an ampule within said container enclosing a bacteria culture, a thread extending around said ampule and through said sterile closure in such manner that when said thread is pulled said ampule will contact the walls of said container and be broken.

3. Container for the preservation of bacteria cultures, said container having a sterile closure, an ampule within said container enclosing a bacteria culture, a thread or wire extending around said ampule and through said sterile closure in such manner that when said thread is pulled said ampule will contact the walls of said container and be broken whereby the contents of said ampule will be discharged into a substratum within said container, said ampule being composed of glass having such internal tensions that it will crush to dust when broken.

4. Container for the preservation of bacteria cultures, said container having a narrow mouth forming a shoulder and a sterile closure, an ampule within said container enclosing a bacteria culture, a thread or wire extending around said ampule .and through said sterile closure, the length of said ampule exceeding the internal width of the mouth of said container, the thread being of such length as to suspend the ampule in contact with the shoulders of said container so that when said thread is pulled said ampule will break against the shoulders of said container.

GUSTAV LARSEN.

REFERENCES CITED The following references are of record in the file of this patent:

UNITED STATES PATENTS Number Name Date 418,271 Buckmaster Dec. 31, 1889 1,332,985 Jarrett Mar. 9, 1920 1,991,938 Houghton Feb. 19, 1935 2,034,986 Mislowitzer Mar. 24, 1936 2,275,567 Smith Mar. 10, 1942

Patent Citations
Cited PatentFiling datePublication dateApplicantTitle
US418271 *May 20, 1889Dec 31, 1889 Ligature-holder
US1332985 *Sep 12, 1919Mar 9, 1920Edwin S JarrettMixing-container
US1991938 *Apr 5, 1926Feb 19, 1935Safety Fumigant CompanyVolatile fumigant package
US2034986 *Dec 29, 1932Mar 24, 1936Mislowitzer ErnstDevice for dispatching swabs, blood samples, and the like for bacteriological and diagnostic purposes
US2275567 *May 22, 1939Mar 10, 1942Arthur E SmithContainer closure
Referenced by
Citing PatentFiling datePublication dateApplicantTitle
US2717226 *Apr 3, 1951Sep 6, 1955Skovsted Aage ThorsenProcess for the manufacture of a rat exterminating compound containing desiccated, storable salmonella bacteria in their virulent condition
US2731965 *Sep 26, 1952Jan 24, 1956 haralson
US2752036 *Mar 29, 1954Jun 26, 1956Parkhurst Jr John CContainer for chemicals
US2776242 *May 21, 1953Jan 1, 1957Asta Werke Ag Chem FabApparatus for providing fresh cultures of living micro-organisms
US2971576 *Sep 30, 1957Feb 14, 1961Anker Willard BMultiple tank assembly
US3068154 *Nov 4, 1959Dec 11, 1962Hill Top Res Inst IncApparatus for preparing a fresh culture of microorganisms
US3616896 *Jul 15, 1969Nov 2, 1971Barton Frederic DCooling system package
US3964643 *Dec 23, 1974Jun 22, 1976L'orealUnpressurized container for holding a plurality of products separately and dispensing them simultaneously
US4304869 *May 27, 1980Dec 8, 1981American Sterilizer CompanyApparatus for rupturing a sealed, frangible container
US4387803 *Nov 21, 1980Jun 14, 1983Mercil Robert AAbsorbent device for containers for sugar, salt, etc.
US4563186 *Apr 5, 1984Jan 7, 1986Purex CorporationMulti-functional laundry product and employment of same during fabric laundering
US4618444 *Sep 17, 1984Oct 21, 1986Purex CorporationHousehold laundry detergent with dual strength bleach
US5710041 *Jun 3, 1996Jan 20, 1998Remel L.P.In situ reconstitutable lyophylized bacteria ampoule package
US5733777 *Jun 6, 1996Mar 31, 1998Dudney; Ralph A.Packaging system for extended shelf life of microbial system
US6818438Sep 30, 2003Nov 16, 2004Becton, Dickinson And CompanyCulture flask
DE2461617A1 *Dec 27, 1974Jul 10, 1975OrealDruckloser behaelter zum voneinander getrennten aufbewahren und gleichzeitigen abgeben wenigstens zweier medien
Classifications
U.S. Classification435/304.2, 435/879, 206/219, 435/260, 215/6, 604/416
International ClassificationB65D83/00, B65D81/32
Cooperative ClassificationY10S435/879, B65D83/0055, B65D81/3222
European ClassificationB65D83/00B, B65D81/32C1