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Publication numberUS3071316 A
Publication typeGrant
Publication dateJan 1, 1963
Filing dateMay 19, 1959
Priority dateMay 19, 1959
Publication numberUS 3071316 A, US 3071316A, US-A-3071316, US3071316 A, US3071316A
InventorsAlexander F Ippolito, Piemonte William John
Original AssigneeLourdes Instr Corp
Export CitationBiBTeX, EndNote, RefMan
External Links: USPTO, USPTO Assignment, Espacenet
Bottle support and cap assembly for centrifuge
US 3071316 A
Abstract  available in
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Claims  available in
Description  (OCR text may contain errors)

Jan. 1, 1963 W. J. PIEMONTE ETAL 3,071,316 BOTTLE SUPPORT AND CAP ASSEMBLY F' OR CENTRIF'UGE Filed May 19, 1959 INVENTORS wu/4M nu/,v Plinn/vrs ALEXANDER F. /PaL/ra United States Patent fltlce Patented Jan. 1, 1963 3,071,316 BOTTLE SUPPORT AND CAP ASSEMBLY FOR CENTRIFUGE William John Piemonte and Alexander F. Ippolito, Brooklyn, N.Y., assignors, by mesne assignments, to Lourdes Instrument Corporation, Brooklyn, N.Y.

Filed May 19, 1959, Ser. No. $14,305 1 Claim. (Cl. 233-26) This invention relates to bottle supports and more fspecifically to a support in combination With a sealing cap assembly for 4a bottle, as mounted in an angle type centrifuge.

The prior art relating to the support of a fragile bottle with a cap assembly is apparently only found in the type of mounting used for a vacuum bottle, in which the inner bottle is securely retained between the outer shell, a Vacuum about the inner bottle and a cap assembly to retain the inner bottle so that it will not be displaced or break. In utilizing a bottle, whether capped or uncapped, in an angle type centrifuge, because of the angle of the rotor compartments and the high speeds and the resultant centrifugal forces involved, all of the presently available plastic bottles have inadequate strength to withstand the centrifugal force when they are filled and capped. Further, the presently available sealing caps do not offer effective sealing during centrifugation.

It is an object of this invention to provide a cap aS- sembly for a fragile bottle, as mounted in the rotor of an -angle type centrifuge.

lt is a further object of this invention to provide a reinforcing supporting assembly for the cap mounted in a fragile bottle that is to be utilized in the rotor of an angle type centrifuge.

Further objects of this invention shall be apparent by reference to the accompanying detailed description and the drawings, in which:

FIG. l is a cross sectional view of a centrifuge rotor, in which a plurality of bottles are mounted in spaced relation in pockets within the rotor, and

FIG. 2 is a cross sectional View of the bottle and supporting cap assembly.

Referring to the drawings, there is illustrated an angle -type centrifuge rotor that is provided with a plurality of pockets 12 (six compartments or pockets in this instance) in which bottles 15 (of approximately 250 ml. or 500 ml.) are mounted. These bottles are at approximately a 28 angle to the vertic-al axis.

When centrifuging with an uncapped plastic bottle, the bottle cannot be filled, since there would be spillage from the top as the fluids in the bottle build up to a vertical circular Wall as the centrifugal force increases during acceleration. When centrifuging with a capped bottle, it has been found that with a rigid bottle such as made of stainless steel, the bottle will stand up under terrific centrifugal force developed Without breaking. In this invention, when centrifugal forces in excess of 20,000 times gravity at speeds in excess of 11,000 r.p.m. are to be considered, and when a polyethylene bottle is to be used and lled and sealed with a cap, the bottle 15, if it is not supported at its neck will distort and may rupture at the neck of the bottle. of bottle that is rather fragile, there is provided a cap assembly 16 for the bottle 15, and a reinforcing support- Therefore, to protect this typeV ing element 17 which may be threadably secured to retain the bottle neck and cap assembly in their proper relationship and prevent rupturing of said botle. The cap assembly is comprised of a stainless steel circular crown member 18 that covers and rests upon the top aperture or mouth of bottle 15. The crown member 18 is provided with a central aperture 19 and on the inner surface of the crown member there is a reduced portion 20 which provides a shoulder 21. A similar type element 22 of a smaller diameter is provided to be inserted within the mouth of said bottle 15, element 22 is similarly provided with a reduced portion 23 an-d a shoulder 24. Element 22 is not provided with a central aperture, but rather is provided with an extended threaded stud 25. A neoprene gasket v26 of a -fairly good thickness is inserted between elements 18 and 22, and with the stud 25 passing upward through the aperture 19 a nut 27 may be threadably secured to the stud 25 to retain the crown element 18 in juxtaposition to the gasket 26. This assembly may be inserted in the mouth of the bottle 15 and by turning nut 27 to draw element 22 toward element l, there will be a squeezing and distor-tion of the gasket 26 to thus provide a tight sealing cap assembly 16. With bottle 15 properly sealed with the cap assembly 16, the bottle 15 may be mounted in a pocket 12 of the rotor. It is apparent that if the rotor were operated at high speed with the bottle cap assembly so mounted, there would be a terrific distortion across the neck area of the bottle 15. Therefore, a supporting element 17 is mounted to surround the neck of the bottle. Supporting element 17 may be comprised of any rigid reinforcing material, such as aluminum, to provide the necessary strength. Element 17 at its base 29 is formed or cut on its inner periphery to fit the contour of the curved upper portion 15A of bottle 15, and the base portion 29, on its periphery, is circular in form, With a diameter to match the diameter of the rotor compartment 12. Element 17 may be formed on its inner periphery of the upper portion 30 -with a mating contour to mate with and match the outer periphery of the neck of bottle 15. The upper end of portion 30 is also provided with a cut out portion 31 to permit the crown member 1S to fit into portion 31 of element 17.

It is to be noted that the base portion 29 of the suppor-ting element 17 fits well within the bore of pocket 12 to provide a good solid support when the bottle 15 is mounted within pocket 12. The rest of the bottle is fully supported within the pocket of the rotor. It is apparent that when the rotor 10 is operated anda considerable centrifugal force is developed, this supporting element and cap assembly provides a rigid support for the polyethylene bottle 15 that is being utilized. With this supporting assembly, it is possible to -use a fairly light polyethylene bottle without danger of rupturing the bottle or losing the cap from the bot-tle due to any distortion.

Although a polyethylene bottle has been described as the preferable type of bottle to be used in this instance in an angle type centrifuge, other types of bottles, that is bottles constructed of other material of insufficient strength may be similarly used, without danger of breaking when supported in the manner illustrated herein, and this invention shall be limited only by the appended cl-airn.

What is claimed is:

In an angle type centrifuge, the combination of a central rotor, and a plurality of fragile distortable bottles that are to be mounted in said central rotor, said bottles having a sealing cap and a reduced neck portion, and a plurality of lightweight supporting elements that are cornposed of a rigid reinforcing material, each one to support the neck of a bottle and said sealing cap, said sealing cap tted and mounted within the neck of each bottle to seal said bottle, said rotor provided with a plurality of apertures, said bottles fitted into said apertures in said rotor, the necks of said bottles extending above said rotor, each supporting element tted to the reduced neck portion and extending slightly above said neck portion, and positioned to lit partially into the aperture in said rotor and each sealing cap abutting with the supporting element Where it extends above said neck portion to prevent distortion of said cap within the neck of said bottle.

References Cited in the le of this patent UNITED STATES PATENTS De Laval June 2l, 1887 Kimmel Mar. 21, 1916 Larsen Nov. 19, 1929 Levy Apr. 23, 1940 Grebmeier Aug. 17, 1948 Schoprneyer Nov. 28, 1950 Allen etal Ian. l1, 1955 Whitby Dec. 31, 1957 Szent-Gyorgyi et al May 13, 1958 Pickels et -al Mar. 24, 1959 FOREIGN PATENTS Switzerland Dec. 17, 1934

Patent Citations
Cited PatentFiling datePublication dateApplicantTitle
US365120 *Jun 21, 1887 de layal
US1176463 *Nov 30, 1914Mar 21, 1916Joseph P KimmelPipe-testing plug.
US1736350 *Oct 25, 1926Nov 19, 1929Forenede Bryggerier AsClosing device for containers made of aluminum or other soft metals
US2198256 *Apr 11, 1938Apr 23, 1940Henri G LevyCentrifuge tube
US2447330 *May 16, 1946Aug 17, 1948Joseph GrebmeierRotor for ultracentrifuge machines
US2531745 *Nov 18, 1947Nov 28, 1950Clifford S SchopmeyerSqueeze-bottle atomizer for acid liquids
US2699289 *Sep 2, 1950Jan 11, 1955Custom Scient Instr IncHigh-speed centrifuge
US2817970 *Jan 2, 1953Dec 31, 1957Pillsbury Mills IncApparatus for determining the particle size distribution of finely divided materials
US2834541 *Sep 20, 1956May 13, 1958Sorvall Inc IvanCentrifuging apparatus and system
US2878992 *Dec 28, 1956Mar 24, 1959Beckman Instruments IncCentrifuge apparatus and rotor therefor
CH172064A * Title not available
Referenced by
Citing PatentFiling datePublication dateApplicantTitle
US3133882 *Jul 21, 1961May 19, 1964Internat Equipment CompanyCentrifuges with retainers, retainers, and bottle stoppers for use therewith
US3219421 *Jan 18, 1965Nov 23, 1965Jr Robert SchwarzTube for use in diagnostic and therapy control
US3265296 *Mar 6, 1964Aug 9, 1966Internat Equipment CompanyPlastic centrifuge bottles and caps therefor
US3447712 *Dec 13, 1965Jun 3, 1969Beckman Instruments IncCentrifuge test tube cap
US3456876 *Mar 23, 1966Jul 22, 1969Beckman Instruments IncApparatus and articles for increasing the rate of particle separation and removal
US3459369 *Sep 16, 1965Aug 5, 1969Beckman Instruments IncCentrifuge test tube cap
US3713775 *Jun 22, 1970Jan 30, 1973Bio Dynamics IncCentrifuge clinical chemistry analysis system
US3938735 *Mar 13, 1975Feb 17, 1976Beckman Instruments, Inc.Capping assembly for thin all centrifuge tubes
US3998383 *Jul 16, 1975Dec 21, 1976E. I. Du Pont De Nemours And CompanyGradient separation apparatus
US4007013 *Jul 9, 1976Feb 8, 1977Contraves AgHolder device for sample vials or the like for an analysis apparatus
US4372483 *May 29, 1981Feb 8, 1983Beckman Instruments, Inc.Fluid containment annulus for fixed angle rotors
US4787971 *Jan 23, 1987Nov 29, 1988Alan DonaldMiniaturized column chromatography separation apparatus and method of assaying biomolecules employing the same
US4830209 *May 9, 1988May 16, 1989Multi-Technology Inc.Fail safe releasible locks for cappped disposable centrifuge containers
US4874102 *Oct 31, 1988Oct 17, 1989Multi-Technology Inc.Medical fail safe releasible locks and/or seals for capped disposable centrifuge containers, cryogenic vials and the like
US4883644 *Dec 9, 1987Nov 28, 1989Brandeis UniversityMicrotube vortexer adapter and method of its use
US4896780 *Nov 14, 1988Jan 30, 1990Multi-Technology Inc.Fail safe releasible locks for capped disposable centrifuge containers
US4902270 *Oct 3, 1988Feb 20, 1990Nalge CompanyHolding a fluid sample
US4953741 *Jul 24, 1989Sep 4, 1990Multi-Technology Inc.Medical fail safe releasible locks and/or seals for capped disposable centrifuge containers, cryogenic vials and the like
US4956103 *Apr 26, 1989Sep 11, 1990Multi-Technology Inc.Fail safe releasible locks for capped disposable centrifuge containers
US5011663 *Aug 13, 1990Apr 30, 1991S E A C S.R.L.Multitest-tube for clinical chemistry analysis for several simultaneous tests
US5127895 *Mar 30, 1990Jul 7, 1992Beckman Instruments, Inc.Self-seal centrifuge tube
US5318749 *May 13, 1992Jun 7, 1994Firma Andreas HettichSlide or disk for holding biological fluid sample in centrifuge vessel during settling and collection of cells on surface
US5395001 *Apr 2, 1993Mar 7, 1995Beckman Instruments, Inc.Supporting spacer for self-sealing centrifuge tubes
US5901873 *Apr 25, 1997May 11, 1999Beckman Instruments, Inc.Self-seating self-sealing labware adapter
US6170690May 14, 1999Jan 9, 2001Shin-Etsu Polymer Co., Ltd.Air-tightly sealable container with bell jar covering
US6350225 *Jun 29, 2000Feb 26, 2002Kendro Laboratory Products, L.P.Support bridge for preventing centrifugal forces from collapsing a container placed in a centrifuge rotor
US8215508Aug 21, 2008Jul 10, 2012Nalge Nunc International CorporationCentrifuge bottle closure and assembly thereof
US20100331163 *Jun 30, 2010Dec 30, 2010Hitachi Koki Co. Ltd.Centrifugal separator
DE8700742U1 *Jan 16, 1987May 14, 1987Herolab Gmbh Laborgeraete, 6837 St. Leon-Rot, DeTitle not available
EP0021599A1 *May 21, 1980Jan 7, 1981Beckman Instruments, Inc.Seal arrangement for a centrifuge rotor cavity
EP0035829A1 *Feb 4, 1981Sep 16, 1981Beckman Instruments, Inc.Supporting cap and spacer for centrifuge tubes
EP2190581A1 *Aug 21, 2008Jun 2, 2010Nalge Nunc International CorporationCentrifuge bottle closure and assembly thereof
WO1994022584A1 *Apr 1, 1994Oct 13, 1994Beckman Instruments IncSupporting spacer for self-sealing centrifuge tubes
WO2001002255A2 *Jun 30, 2000Jan 11, 2001Kendro Lab Products L PA container assembly having a support bridge
Classifications
U.S. Classification494/16, 215/44, 422/915, 422/918, 215/42, 422/72, 220/640, 220/236, 422/548
International ClassificationB04B5/04, G01N33/483, B01L3/14, G01N35/04, G01N21/07, B04B9/10
Cooperative ClassificationB04B9/10, B04B2007/025, B01L3/5021, B04B5/0421, G01N21/07, B01L3/5453, G01N2035/0436, B04B5/0414
European ClassificationB01L3/5453, B01L3/5021, B04B5/04B2, B04B5/04B2B, B04B9/10, G01N21/07