Search Images Maps Play YouTube News Gmail Drive More »
Sign in
Screen reader users: click this link for accessible mode. Accessible mode has the same essential features but works better with your reader.

Patents

  1. Advanced Patent Search
Publication numberUS3191476 A
Publication typeGrant
Publication dateJun 29, 1965
Filing dateMar 1, 1962
Priority dateMar 1, 1962
Publication numberUS 3191476 A, US 3191476A, US-A-3191476, US3191476 A, US3191476A
InventorsMccormick James B
Original AssigneeAmes Atomium Inc
Export CitationBiBTeX, EndNote, RefMan
External Links: USPTO, USPTO Assignment, Espacenet
Anti-roll device
US 3191476 A
Abstract  available in
Images(1)
Previous page
Next page
Claims  available in
Description  (OCR text may contain errors)

June 29, 1965 amcoRMlck 3,191,476

ANTI-ROLL DEVICE Filed March 1. 1962 FLUID SUPPLY 1 INVENTOR.

M My 4110 United States Patent 3,191,476 ANTI-ROLL DEVICE James B. McCormick, La Grange, 11]., assignor, by mesne assignments, to Arnes Atornium, Inc., Billerica, Mass, a corporation of Massachusetts Filed Mar. 1, 1962, Ser. No. 176,751 7 Claims. (Cl. 83-469) This invention relates principally to the preparation of tissue sections for microscopic examination and, more particularly, to an improved method of slicing such a section from a tissue specimen.

The invention also relates to a device particularly adapted to carry out the improved method.

In order to microscopically examine an organic specimen such as, for example, a tissue specimen obtained through a biopsy or the like, it is necessary to prepare thin slices of the specimen, these slices being known as sections. The sections have a thickness range from about 2 to 25 microns and are sliced from the specimen by means of a microtome instrument which conventionally includes a movable carrier, on which the specimen is mounted, and a stationary knife blade disposed adjacent the path of movement of the carrier. The carrier is reciprocated past the knife as well as advanced toward the knife, by means of a crank, and a section is sliced from the specimen by the knife during a portion of the movement of the carrier.

As the section is sliced from the specimen and moves away from the cutting edge of the knife, it frequently curls or rolls into a cylinder, a condition which renders it unsuitable for mounting in slides or the like. While a curled section can occasionally be straightened and used, it generally breaks when an attempt is made to uncurl it because of its thinness and consequent low strength. As a general rule, therefore, a section which has curled is unusable.

The curling of a section generally results from improper preparation of the specimen or improper adjustment of the instrument, but both of these factors are so critical that the slicing of a section in which curling does not occur generally requires the services of a technician of considerable experience. For a technician of modest training, the proper slicing of a section is approached on a trial and error basis, with consequent expense of both time and effort. In surgical pathology, however, where a patient is frequently maintained under anesthetic on the operating table pending the results of the microscopic examination, a time-consuming method of preparing the section cannot be tolerated.

One common solution to the rolling of the section concerns the use of a soft brush to assist the section as it leaves the knife. However, this technique requires a very delicate touch on the part of the technician and is generally not at all satisfactory in routine use.

Accordingly, the principal object of the present invention is to provide an improved method of slicing a thin section from a specimen.

An additional object of the invention to provide a device which will slice a section from a specimen without curling of the section.

Other objects and advantages of the invention will become apparent with reference to the following description and accompanying drawings in which:

FIGURE 1 is an isometric view of a microtome provided with means for accomplishing the method of the present invention; 7

FIGURE 2 is a fragmentary elevational view of the specimen carrier of the microtome as viewed in the direction of the arrows 2-2 of FIGURE 1; and

FIGURES 3 and 4 are diagrammatic views showing the carrier of FIGURE 2, partially in section, as it moves the specimen into engagement with the knife of the microtome.

. Patented June 29, 1965 While it is recognized that the preparation of the sections, particularly tissue sections, generally involves a detailed procedure which includes such steps as the preparation of the specimen, and the mounting of the specimen on the microtome, etc., the present invention is directed principally to that step in which the section is sliced from the specimen. In accordance with the method of the invention (see FIGS. 3 and 4), a stream of gaseous fluid is directed toward the section as it is formed so as to urge it against the face of the knife and thereby prevent its curling. Preferably, the gaseous fluid is directed across the surface of the specimen toward that edge of the specimen which is the first to be contacted by the knife so that the stream of fluid will engage each portion of the section immediately upon its being formed, the section suitable results. When the knife is slicing the section from the specimen, therefore, the fluid will flow across the outer face of the knife and will flatten the section against the face of the knife.

Also, it is found preferable to use a fluid below ambient temperature, especially when the specimen is frozen, and air from the cold well of a cryostat has been found to be satisfactory for this purpose.

The device provided for carrying out the above-method is illustrated generally in FIGURE 1 and comprises a microtome 11 which includes a generally rectangular base 13, on the rearward portion of which is carried a box-like housing 15 having an elongated vertically disposed opening 17 in its forward wall through which a specimen carrier 19 projects outwardly. The carrier is connected-to an operating mechanism (not shown) enclosed by the housing 15 which raises and lowers the carrier and advances it forwardly when a handle 21, rotatably mounted on a side of the housing, is turned manually.

The carrier 19 comprises a generally cylindrical shaft 23 bored axially into its forward face to provide a cavity 25 within which a specimen 27, embedded in the forward face of a rectangular paraffin block 29, is carried. The block 29 is of such a size that the specimen projects outwardly of the cavity 25 in overhanging relation to a knife blade 31, hereinafter described, and is maintained within the cavity by a clamping plate 33 urged against the upper surface of the paraffin block 29 by an adjustable screw 35.

A knife support 37 is carried in a guideway 39 on the forward portion of the base 13 so as to be movable in the guideway to an out of the way position as, for example, when the knife blade 31 is to be replaced or cleaned. The blade 31 is carried at the upper end of the support 37 and is secured in place by set screws 41 which permit the blade -to be adjusted to various angles relative to the vertical as the occasion may demand.

In order to carry out the method of the present invention, a nozzle 43 is secured to the upper surface of the carrier shaft 23 by means of a clip 45, and is connected by a flexible hose 47 to a supply of gaseous fluid as, for example, a compressor in communication with the cold well of a cryostat. The nozzle 43 is positioned so that it will direct a jet of fluid toward the face of the specimen at an angle of approximately 45 degrees and, to ensure that its disposition will remain relatively fixed, preferably includes a neck 49 formed of a flexible metal tubing.

As can be seen best in FIGURES 3 and 4, the fluid is directed toward the surface of the specimen and, as the carrier is lowered so as to bring the specimen into engagement with the knife blade 31, is deflected by the specimen and flows downwardly along the forward face of the blade. As the section is being formed (FIG. 4), the flow of fluid urges the section against the face of the blade and prevents the section from curling.

While the preferred form of the device for effecting the method has been shown in the form of a microtome having a nozzle 43 mounted on the carrier so as to travel with the carrier as it is raised and lowered, it should be apparent that the method could be effected by mounting the nozzle on other portions of the microtome such as, for example, on the knife support 37. In the latter instance, of course, the nozzle would remain stationary during the operation of the device.

While various features of the invention have been shown and described, it should be apparent that various modifications may be made therein without departing from the scope of the invention as set forth in the following claims.

What is claimed is:

1. The method of providing a thin section of a specimen for use in microscopic examination, which method includes the step of slicing a generally planar uniform thin section from a surface of the specimen by means of a knife while directing a stream of gaseous fluid toward that edge of the specimen which is the first to be contacted by the knife so as to urge the section against the face of the knife as the section is being formed and thereby minimize curling thereof.

2. The method of providing a thin section of a specimen for use in microscopic examination, which method includes the step of slicing the section from a surface of the specimen by means of a knife while directing a stream of gaseous fluid having a temperature less than ambient toward that edge of the specimen which is the first to be contacted by the knife, said fluid being directed at an angle acute to the surfaceof the specimen and in a direction which will urge the section against the face of the knife as the section is being formed so as to thereby minimize curling thereof.

3. The method of providing a thin section of a specimen for use in microscopic examination, which method includes the step of slicing the section from a surface of the specimen by means of a knife while directing a stream of gaseous fluid having a temperature less than ambient toward that edge of the specimen which is the first to be contacted by the knife, said fluid being directed at an angle of approximately 45 degrees to the surface of the specimen in a direction which will urge the section against the face of the knife as the section is being formed so as to thereby minimize curling thereof.

4. In combination, a microtome including a specimen carrier and a knife adjacent said carrier adapted to slice sections from the surface of the specimen incident to predetermined relative reciprocal movement between said carrier and knife, fluid-dispensing means connected to a source of gaseous fluid under pressure, and means for mounting said fluid-dispensing means so as to cause it to direct a stream of gaseous fluid toward that edge of the specimen which is the first to be contacted by the knife so as to cause the stream to flow over the section and urge the section against the face of the knife as the section is being formed, thereby overcoming the tendency of the section to curl.

5. In combination, a microtome including a specimen carrier and a knife adjacent said carrier adapted to slice sections from the surface of the specimen carried thereby incident to predetermined relative movement between said carrier and knife, fluid-dispensing means, means for mounting said fluid dispensing means so as to direct a stream of gaseous fluid toward that edge of the specimen which is the first to be contacted by the knife, said fluid being directed by said fluid-dispensing means at an angle acute to the surface of the specimen and in a direction which will urge the section against the face of the knife while the section is being formed to thereby minimize curling thereof, and means connecting said nozzle to a supply of gaseous fluid having a temperature less than ambient.

6. In combination, a microtome including a specimen carrier and a knife adjacent said carrier adapted to slice sections from the surface of the specimen carried thereby incident to predetermined relative movement between said carrier and knife, a fluid-dispensing nozzle, means for mounting said nozzle so as to direct a stream of gaseous fluid to 'ard that edge of the surface of the specimen which is the first to be contacted by the knife, said fluid being directed at an angle of approximately 45 degrees to the surface of the specimen and in a direction which will urge the section against the face of the knife while the section is being formed, and means connecting said nozzle to a supply of air having a temperature less than ambient.

7. In combination, a microtome including a specimen carrier and a knife adjacent said carrier adapted to slice sections from the surface of the specimen when said carrier is moved relative to the edge of said knife, a fluiddispensing nozzle, means for mounting said nozzle on said carrier so as to direct a stream of fluid dispensed therefrom toward that edge of the surface of the specimen which is the first to be contacted by the knife, said fluid thereby flowing across the face of the specimen toward said edge while said section is being formed, and means connecting said nozzle to a supply of air under a pressure greater than atmospheric and of a temperature less-than ambient.

References Cited by the Examiner UNITED STATES PATENTS 1,648,938 11/27 Dietrick 83169 2,061,581 11/36 Lippincott 8250 2,288,709 7/42 Hood 146241 2,662,445 12/53 Jacoby 83915.5

ANDREW R. JUHASZ, Primary Examiner.

CARL W. TOMLIN, Examiner.

Patent Citations
Cited PatentFiling datePublication dateApplicantTitle
US1648938 *Nov 4, 1926Nov 15, 1927Greenville Steel CarMethod and apparatus for cooling friction saws
US2061581 *Dec 5, 1934Nov 24, 1936Ideal Roller & Mfg CompanyApparatus for trimming cylinders
US2288709 *Apr 13, 1938Jul 7, 1942Hood George JDermatome and method of excising skin
US2662445 *Aug 17, 1950Dec 15, 1953Jacoby Jr George WMicrotome knife
Referenced by
Citing PatentFiling datePublication dateApplicantTitle
US3293966 *Sep 29, 1964Dec 27, 1966Miles LabMicrotome anti-roll device
US3667330 *Dec 16, 1970Jun 6, 1972Devco IncMicrotome assembly
US3732768 *Apr 6, 1971May 15, 1973Nat Res DevMicrotome knife mount
US5713255 *Jul 5, 1993Feb 3, 1998Microm Laborgerate GmbhProcess and apparatus for the production of thin sections by means of a microtome
US5865081 *Mar 27, 1995Feb 2, 1999Sakura Finetek U.S.A., Inc.Anti-roll rake
US5988029 *Sep 29, 1997Nov 23, 1999Microm Laborgerate GmbhHeight-adjustable table for a microtome
US6058824 *Sep 2, 1997May 9, 2000Leica Instruments GmbhCutter holder for accommodating wedge-shaped microtome cutters
US7802507 *Jan 10, 2007Sep 28, 2010Seiko Instruments Inc.Automatic sliced piece fabricating apparatus and automatic sliced piece sample fabricating apparatus
US8048206Jan 6, 2009Nov 1, 2011Leica Biosystems Nussloch GmbhApparatus and method for filtering sectioning wastes of a microtome that are present in an air stream
US8152906Aug 30, 2011Apr 10, 2012Leica Biosystems Nussloch GmbhApparatus and method for filtering sectioning wastes of a microtome that are present in an air stream
US8172927Aug 30, 2011May 8, 2012Leica Biosystems Nussloch GmbhApparatus and method for filtering sectioning wastes of a microtome that are present in an air stream
US8728799Jan 6, 2009May 20, 2014Leica Biosystems Nussloch GmbhApparatus and method for isolating histological sections produced with a microtome
US8765401Dec 22, 2008Jul 1, 2014Leica Biosystems Nussloch GmbhMethod and apparatus for producing histological sections with a microtome
US8850848Jun 8, 2012Oct 7, 2014Leica Biosystems Nussloch GmbhApparatus for sectioning histological samples having a spiral-shaped capillary tube
CN1036944C *Jul 9, 1993Jan 7, 1998迈克罗姆实验室用具有限公司Method and apparatus for producing thin plate by use of cutter blade
DE10325945A1 *Jun 7, 2003Jan 5, 2005Leica Mikrosysteme GmbhErgonomische Vorrichtung zum Schneiden von Präparaten
DE19815696A1 *Apr 8, 1998Oct 21, 1999Gsf Forschungszentrum UmweltTemperaturgeregelte Überströmapparatur für biologische Proben
DE19815696C2 *Apr 8, 1998Jun 29, 2000Gsf Forschungszentrum UmweltTemperaturgeregelte Überströmapparatur für biologische Proben
DE102008000035A1 *Jan 10, 2008Jul 23, 2009Leica Biosystems Nussloch GmbhVorrichtung und Verfahren zur Vereinzelung von mit einem Mikrotom angefertigten histologischen Schnitten
DE102008000035B4 *Jan 10, 2008Feb 24, 2011Leica Biosystems Nussloch GmbhVorrichtung zur Vereinzelung von mit einem Mikrotom angefertigten histologischen Schnitten
DE102008047415A1 *Sep 16, 2008Apr 15, 2010Leica Biosystems Nussloch GmbhMikrotom zum Herstellen von Schnitten eines Objekts
DE102008047415B4 *Sep 16, 2008Dec 2, 2010Leica Biosystems Nussloch GmbhMikrotom zum Herstellen von Schnitten eines Objekts
DE102011051097A1 *Jun 16, 2011Dec 20, 2012Leica Biosystems Nussloch GmbhVorrichtung zum Schneiden von histologischen Proben mit wendelförmigem Kapillarrohr
DE102011051097B4 *Jun 16, 2011Aug 8, 2013Leica Biosystems Nussloch GmbhMikrotom zum Schneiden von histologischen Proben mit wendelförmigem Kapillarrohr
WO1994001751A1 *Jul 5, 1993Jan 20, 1994Ctl Diehm IntProcess and device for producing thin sections by means of a microtome
WO1994028390A1 *Jun 2, 1994Dec 8, 1994Herve TaboneSuction microtome, especially for histological research and the like
Classifications
U.S. Classification83/169, 83/915.5, D24/216
International ClassificationG01N1/04, G01N1/06
Cooperative ClassificationG01N1/06
European ClassificationG01N1/06